Bcat2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bcat2
Entrez Gene ID	64203
Full Name	branched chain amino acid transaminase 2
Synonyms	BCT2,Bcatm,mBcat
General protein information	Preferred Names branched chain amino acid transaminase 2 Names branched-chain-amino-acid aminotransferase, mitochondrial BCAT(m) branched chain amino acid transaminase 2, mitochondrial branched chain aminotransferase 2, mitochondrial heart branched chain aminotransferase precursor encoding mitochondrial protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	mitochondrial branched-chain-amino-acid transaminase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589657.1	XP_017445146.1	branched-chain-amino-acid aminotransferase, mitochondrial isoform X2
XM_008759413.1	XP_008757635.1	branched-chain-amino-acid aminotransferase, mitochondrial isoform X1
XM_006229149.1	XP_006229211.1	branched-chain-amino-acid aminotransferase, mitochondrial isoform X2
NM_022400.1	NP_071795.1	branched-chain-amino-acid aminotransferase, mitochondrial precursor

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A10307g	XP_451451.1
Schizosaccharomyces pombe (fission yeast)	eca39	NP_595180.2
Magnaporthe oryzae (rice blast fungus)	MGG_02489	XP_003709286.1
Neurospora crassa	NCU04754	XP_960287.1
Pan troglodytes (chimpanzee)	BCAT2	XP_001171838.2
Canis lupus familiaris (dog)	BCAT2	XP_541509.3
Bos taurus (cattle)	BCAT2	NP_001013611.2
Mus musculus (house mouse)	Bcat2	NP_033867.1
Oryza sativa (rice)	Os03g0231600	NP_001049461.1
Rattus norvegicus (Norway rat)	Bcat2	NP_071795.1
Saccharomyces cerevisiae (baker's yeast)	BAT1	NP_012078.3
Xenopus tropicalis (tropical clawed frog)	bcat2	NP_001006740.1
Homo sapiens (human)	BCAT2	NP_001181.2
Macaca mulatta (Rhesus monkey)	BCAT2	XP_001114272.1
Danio rerio (zebrafish)	bcat2	NP_001002676.1
Arabidopsis thaliana (thale cress)	BCAT-2	NP_001031015.1

Gene Ontology
Bibliography

Related articles in PubMed

Ketoisocaproic acid, a metabolite of leucine, suppresses insulin-stimulated glucose transport in skeletal muscle cells in a BCAT2-dependent manner.
Moghei M, Tavajohi-Fini P, Beatty B, Adegoke OA
American journal of physiology. Cell physiology311(3)C518-27(2016 09)

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)

Alcohol-induced IGF-I resistance is ameliorated in mice deficient for mitochondrial branched-chain aminotransferase.
Lang CH, Lynch CJ, Vary TC
The Journal of nutrition140(5)932-8(2010 May)

Branched-chain amino acid metabolon: interaction of glutamate dehydrogenase with the mitochondrial branched-chain aminotransferase (BCATm).
Islam MM, Nautiyal M, Wynn RM, Mobley JA, Chuang DT, Hutson SM
The Journal of biological chemistry285(1)265-76(2010 Jan)

Disruption of BCATm in mice leads to increased energy expenditure associated with the activation of a futile protein turnover cycle.
She P, Reid TM, Bronson SK, Vary TC, Hajnal A, Lynch CJ, Hutson SM
Cell metabolism6(3)181-94(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bcat2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bcat2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13079	NM_022400.1 Latest version!	Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa22780	XM_017589657.1 Latest version!	Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa22780	XM_006229149.1 Latest version!	Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa43571	XM_008759413.1 Latest version!	Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bcat2 ( NM_022400.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071795.1 Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), mRNA.

Price & Availability↑

CloneID	ORa13079
Clone ID Related Accession (Same CDS sequence)	NM_022400.1
Accession Version	NM_022400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	branched-chain-amino-acid aminotransferase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U68417.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U68417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCTGCAG CTATACTAGG ACAGGTCTGG ACCCGGAAGC TTCTGCCTAT TCCCTGGCGT 
CTATGTGTGC CCGGAAGATG TGTGTCCTCC AACTTCAAGG CGGCAGACCT CCAGGTTCAG 
GTGACCAGAG AGCCACAGAA GAAGCCAGCC CCTAGCCAGC CTCTGCTGTT TGGGAAGACA 
TTCACGGACC ACATGCTGAT GGTGGAGTGG AACAGCAAGA CAGGCTGGGG CCCTCCAAGG 
ATCCAGCCCT TCCAGAACCT CACACTGCAC CCAGCCTGCT CCGGCCTGCA CTACTCATTG 
CAGCTCTTTG AAGGCTTGAA AGCTTATAAA GGCAGAGACA AGCAGGTACG CCTCTTCCGG 
CCCTGGCTCA ACATGGACAG GATGCTACGT TCTGCAAGGC GCCTCTGCCT GCCAGACTTT 
GACAAGCAGG AACTGCTGGA GTGCATCCGC CAGCTCATTG AAGTAGACAA AGACTGGGTT 
CCCGATGGCA ATGGAACAAG CCTCTATGTG CGGCCGGTGC TTATTGGAAA TGAGCCCTCC 
CTGGGTGTTG GAATGGTCAC ACAAGCCCTC CTGTTCGTCA TTCTGTGCCC TGTGGGCTCC 
TACTTCCCCG GAGACTCCAT GACACCTGTC TCCCTCCTGG CGGACCCCTC ATTCGTCAGA 
GCCTGGATTG GTGGGGTCGG TGACTGCAAG TTGGGGGGGA ATTACGGCCC CACTGTGGCT 
GTGCAACAAG AAGCCCAGAA GAAAGGCTGC GAGCAGGTCC TCTGGCTGTA CGGGCCGGAC 
CACCAGCTCA CCGAGGTGGG AACCATGAAC ATCTTTGTCT ACTGGACTCA TGAAGACGGA 
GAACTGGAGC TGGCAACCCC CCCACTGGAT GGCATCATCC TCCCCGGAGT GGTTCGACAA 
AGCCTGCTGG ATCTGGCCAG GACTTGGGGT GAGTTCCGGG TGGCAGAACG CAAGGTCACT 
ATGAAGGAAT TGAAGCGGGC ACTGGAGGAG GGCCGGGTGC GAGAAGTCTT CGGCTCAGGC 
ACTGCTTGTC AGGTCTGCCC AGTGCACCAA ATCCTATATG AAGGCAAGCA ACTCCACATT 
CCTACCATGG AAAATGGGCC GGAACTCATC CTGCGCTTCC AGAAGGAGTT GAAGGCTATT 
CAGTACGGAA CCAGTGCCCA CGATTGGATG CTTCGTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071795.1
CDS	8..1189
Translation	MSAAILGQVWTRKLLPIPWRLCVPGRCVSSNFKAADLQVQVTREPQKKPAPSQPLLFGKTFTDHMLMVEWNSKTGWGPPRIQPFQNLTLHPACSGLHYSLQLFEGLKAYKGRDKQVRLFRPWLNMDRMLRSARRLCLPDFDKQELLECIRQLIEVDKDWVPDGNGTSLYVRPVLIGNEPSLGVGMVTQALLFVILCPVGSYFPGDSMTPVSLLADPSFVRAWIGGVGDCKLGGNYGPTVAVQQEAQKKGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGELELATPPLDGIILPGVVRQSLLDLARTWGEFRVAERKVTMKELKRALEEGRVREVFGSGTACQVCPVHQILYEGKQLHIPTMENGPELILRFQKELKAIQYGTSAHDWMLRV

Target ORF information:

RefSeq Version	NM_022400.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022400.1

ORF Insert Sequence:

ATGTCTGCAG CTATACTAGG ACAGGTCTGG ACCCGGAAGC TTCTGCCTAT TCCCTGGCGT 
CTATGTGTGC CCGGAAGATG TGTGTCCTCC AACTTCAAGG CGGCAGACCT CCAGGTTCAG 
GTGACCAGAG AGCCACAGAA GAAGCCAGCC CCTAGCCAGC CTCTGCTGTT TGGGAAGACA 
TTCACGGACC ACATGCTGAT GGTGGAGTGG AACAGCAAGA CAGGCTGGGG CCCTCCAAGG 
ATCCAGCCCT TCCAGAACCT CACACTGCAC CCAGCCTGCT CCGGCCTGCA CTACTCATTG 
CAGCTCTTTG AAGGCTTGAA AGCTTATAAA GGCAGAGACA AGCAGGTACG CCTCTTCCGG 
CCCTGGCTCA ACATGGACAG GATGCTACGT TCTGCAAGGC GCCTCTGCCT GCCAGACTTT 
GACAAGCAGG AACTGCTGGA GTGCATCCGC CAGCTCATTG AAGTAGACAA AGACTGGGTT 
CCCGATGGCA ATGGAACAAG CCTCTATGTG CGGCCGGTGC TTATTGGAAA TGAGCCCTCC 
CTGGGTGTTG GAATGGTCAC ACAAGCCCTC CTGTTCGTCA TTCTGTGCCC TGTGGGCTCC 
TACTTCCCCG GAGACTCCAT GACACCTGTC TCCCTCCTGG CGGACCCCTC ATTCGTCAGA 
GCCTGGATTG GTGGGGTCGG TGACTGCAAG TTGGGGGGGA ATTACGGCCC CACTGTGGCT 
GTGCAACAAG AAGCCCAGAA GAAAGGCTGC GAGCAGGTCC TCTGGCTGTA CGGGCCGGAC 
CACCAGCTCA CCGAGGTGGG AACCATGAAC ATCTTTGTCT ACTGGACTCA TGAAGACGGA 
GAACTGGAGC TGGCAACCCC CCCACTGGAT GGCATCATCC TCCCCGGAGT GGTTCGACAA 
AGCCTGCTGG ATCTGGCCAG GACTTGGGGT GAGTTCCGGG TGGCAGAACG CAAGGTCACT 
ATGAAGGAAT TGAAGCGGGC ACTGGAGGAG GGCCGGGTGC GAGAAGTCTT CGGCTCAGGC 
ACTGCTTGTC AGGTCTGCCC AGTGCACCAA ATCCTATATG AAGGCAAGCA ACTCCACATT 
CCTACCATGG AAAATGGGCC GGAACTCATC CTGCGCTTCC AGAAGGAGTT GAAGGCTATT 
CAGTACGGAA CCAGTGCCCA CGATTGGATG CTTCGTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcat2 ( XM_017589657.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445146.1 Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa22780
Clone ID Related Accession (Same CDS sequence)	XM_017589657.1 , XM_006229149.1
Accession Version	XM_017589657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGG TGGAGTGGAA CAGCAAGACA GGCTGGGGCC CTCCAAGGAT CCAGCCCTTC 
CAGAACCTCA CACTGCACCC AGCCTGCTCC GGCCTGCACT ACTCATTGCA GCTCTTTGAA 
GGCTTGAAAG CTTATAAAGG CAGAGACAAG CAGGTACGCC TCTTCCGGCC CTGGCTCAAC 
ATGGACAGGA TGCTACGTTC TGCAAGGCGC CTCTGCCTGC CAGACTTTGA CAAGCAGGAA 
CTGCTGGAGT GCATCCGCCA GCTCATTGAA GTAGACAAAG ACTGGGTTCC CGATGGCAAT 
GGAACAAGCC TCTATGTGCG GCCGGTGCTT ATTGGAAATG AGCCCTCCCT GGGTGTTGGA 
ATGGTCACAC AAGCCCTCCT GTTCGTCATT CTGTGCCCTG TGGGCTCCTA CTTCCCCGGA 
GACTCCATGA CACCTGTCTC CCTCCTGGCG GACCCCTCAT TCGTCAGAGC CTGGATTGGT 
GGGGTCGGTG ACTGCAAGTT GGGGGGGAAT TACGGCCCCA CTGTGGCTGT GCAACAAGAA 
GCCCAGAAGA AAGGCTGCGA GCAGGTCCTC TGGCTGTACG GGCCGGACCA CCAGCTCACC 
GAGGTGGGAA CCATGAACAT CTTTGTCTAC TGGACTCATG AAGACGGAGA ACTGGAGCTG 
GCAACCCCCC CACTGGATGG CATCATCCTC CCCGGAGTGG TTCGACAAAG CCTGCTGGAT 
CTGGCCAGGA CTTGGGGTGA GTTCCGGGTG GCAGAACGCA AGGTCACTAT GAAGGAATTG 
AAGCGGGCAC TGGAGGAGGG CCGGGTGCGA GAAGTCTTCG GCTCAGGCAC TGCTTGTCAG 
GTCTGCCCAG TGCACCAAAT CCTATATGAA GGCAAGCAAC TCCACATTCC TACCATGGAA 
AATGGGCCGG AACTCATCCT GCGCTTCCAG AAGGAGTTGA AGGCTATTCA GTACGGAACC 
AGTGCCCACG ATTGGATGCT TCGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445146.1
CDS	397..1386
Translation	MLMVEWNSKTGWGPPRIQPFQNLTLHPACSGLHYSLQLFEGLKAYKGRDKQVRLFRPWLNMDRMLRSARRLCLPDFDKQELLECIRQLIEVDKDWVPDGNGTSLYVRPVLIGNEPSLGVGMVTQALLFVILCPVGSYFPGDSMTPVSLLADPSFVRAWIGGVGDCKLGGNYGPTVAVQQEAQKKGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGELELATPPLDGIILPGVVRQSLLDLARTWGEFRVAERKVTMKELKRALEEGRVREVFGSGTACQVCPVHQILYEGKQLHIPTMENGPELILRFQKELKAIQYGTSAHDWMLRV

Target ORF information:

RefSeq Version	XM_017589657.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589657.1

ORF Insert Sequence:

ATGCTGATGG TGGAGTGGAA CAGCAAGACA GGCTGGGGCC CTCCAAGGAT CCAGCCCTTC 
CAGAACCTCA CACTGCACCC AGCCTGCTCC GGCCTGCACT ACTCATTGCA GCTCTTTGAA 
GGCTTGAAAG CTTATAAAGG CAGAGACAAG CAGGTACGCC TCTTCCGGCC CTGGCTCAAC 
ATGGACAGGA TGCTACGTTC TGCAAGGCGC CTCTGCCTGC CAGACTTTGA CAAGCAGGAA 
CTGCTGGAGT GCATCCGCCA GCTCATTGAA GTAGACAAAG ACTGGGTTCC CGATGGCAAT 
GGAACAAGCC TCTATGTGCG GCCGGTGCTT ATTGGAAATG AGCCCTCCCT GGGTGTTGGA 
ATGGTCACAC AAGCCCTCCT GTTCGTCATT CTGTGCCCTG TGGGCTCCTA CTTCCCCGGA 
GACTCCATGA CACCTGTCTC CCTCCTGGCG GACCCCTCAT TCGTCAGAGC CTGGATTGGT 
GGGGTCGGTG ACTGCAAGTT GGGGGGGAAT TACGGCCCCA CTGTGGCTGT GCAACAAGAA 
GCCCAGAAGA AAGGCTGCGA GCAGGTCCTC TGGCTGTACG GGCCGGACCA CCAGCTCACC 
GAGGTGGGAA CCATGAACAT CTTTGTCTAC TGGACTCATG AAGACGGAGA ACTGGAGCTG 
GCAACCCCCC CACTGGATGG CATCATCCTC CCCGGAGTGG TTCGACAAAG CCTGCTGGAT 
CTGGCCAGGA CTTGGGGTGA GTTCCGGGTG GCAGAACGCA AGGTCACTAT GAAGGAATTG 
AAGCGGGCAC TGGAGGAGGG CCGGGTGCGA GAAGTCTTCG GCTCAGGCAC TGCTTGTCAG 
GTCTGCCCAG TGCACCAAAT CCTATATGAA GGCAAGCAAC TCCACATTCC TACCATGGAA 
AATGGGCCGG AACTCATCCT GCGCTTCCAG AAGGAGTTGA AGGCTATTCA GTACGGAACC 
AGTGCCCACG ATTGGATGCT TCGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcat2 ( XM_006229149.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229211.1 Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22780
Clone ID Related Accession (Same CDS sequence)	XM_017589657.1 , XM_006229149.1
Accession Version	XM_006229149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGG TGGAGTGGAA CAGCAAGACA GGCTGGGGCC CTCCAAGGAT CCAGCCCTTC 
CAGAACCTCA CACTGCACCC AGCCTGCTCC GGCCTGCACT ACTCATTGCA GCTCTTTGAA 
GGCTTGAAAG CTTATAAAGG CAGAGACAAG CAGGTACGCC TCTTCCGGCC CTGGCTCAAC 
ATGGACAGGA TGCTACGTTC TGCAAGGCGC CTCTGCCTGC CAGACTTTGA CAAGCAGGAA 
CTGCTGGAGT GCATCCGCCA GCTCATTGAA GTAGACAAAG ACTGGGTTCC CGATGGCAAT 
GGAACAAGCC TCTATGTGCG GCCGGTGCTT ATTGGAAATG AGCCCTCCCT GGGTGTTGGA 
ATGGTCACAC AAGCCCTCCT GTTCGTCATT CTGTGCCCTG TGGGCTCCTA CTTCCCCGGA 
GACTCCATGA CACCTGTCTC CCTCCTGGCG GACCCCTCAT TCGTCAGAGC CTGGATTGGT 
GGGGTCGGTG ACTGCAAGTT GGGGGGGAAT TACGGCCCCA CTGTGGCTGT GCAACAAGAA 
GCCCAGAAGA AAGGCTGCGA GCAGGTCCTC TGGCTGTACG GGCCGGACCA CCAGCTCACC 
GAGGTGGGAA CCATGAACAT CTTTGTCTAC TGGACTCATG AAGACGGAGA ACTGGAGCTG 
GCAACCCCCC CACTGGATGG CATCATCCTC CCCGGAGTGG TTCGACAAAG CCTGCTGGAT 
CTGGCCAGGA CTTGGGGTGA GTTCCGGGTG GCAGAACGCA AGGTCACTAT GAAGGAATTG 
AAGCGGGCAC TGGAGGAGGG CCGGGTGCGA GAAGTCTTCG GCTCAGGCAC TGCTTGTCAG 
GTCTGCCCAG TGCACCAAAT CCTATATGAA GGCAAGCAAC TCCACATTCC TACCATGGAA 
AATGGGCCGG AACTCATCCT GCGCTTCCAG AAGGAGTTGA AGGCTATTCA GTACGGAACC 
AGTGCCCACG ATTGGATGCT TCGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229211.1
CDS	377..1366
Translation	MLMVEWNSKTGWGPPRIQPFQNLTLHPACSGLHYSLQLFEGLKAYKGRDKQVRLFRPWLNMDRMLRSARRLCLPDFDKQELLECIRQLIEVDKDWVPDGNGTSLYVRPVLIGNEPSLGVGMVTQALLFVILCPVGSYFPGDSMTPVSLLADPSFVRAWIGGVGDCKLGGNYGPTVAVQQEAQKKGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGELELATPPLDGIILPGVVRQSLLDLARTWGEFRVAERKVTMKELKRALEEGRVREVFGSGTACQVCPVHQILYEGKQLHIPTMENGPELILRFQKELKAIQYGTSAHDWMLRV

Target ORF information:

RefSeq Version	XM_006229149.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229149.1

ORF Insert Sequence:

ATGCTGATGG TGGAGTGGAA CAGCAAGACA GGCTGGGGCC CTCCAAGGAT CCAGCCCTTC 
CAGAACCTCA CACTGCACCC AGCCTGCTCC GGCCTGCACT ACTCATTGCA GCTCTTTGAA 
GGCTTGAAAG CTTATAAAGG CAGAGACAAG CAGGTACGCC TCTTCCGGCC CTGGCTCAAC 
ATGGACAGGA TGCTACGTTC TGCAAGGCGC CTCTGCCTGC CAGACTTTGA CAAGCAGGAA 
CTGCTGGAGT GCATCCGCCA GCTCATTGAA GTAGACAAAG ACTGGGTTCC CGATGGCAAT 
GGAACAAGCC TCTATGTGCG GCCGGTGCTT ATTGGAAATG AGCCCTCCCT GGGTGTTGGA 
ATGGTCACAC AAGCCCTCCT GTTCGTCATT CTGTGCCCTG TGGGCTCCTA CTTCCCCGGA 
GACTCCATGA CACCTGTCTC CCTCCTGGCG GACCCCTCAT TCGTCAGAGC CTGGATTGGT 
GGGGTCGGTG ACTGCAAGTT GGGGGGGAAT TACGGCCCCA CTGTGGCTGT GCAACAAGAA 
GCCCAGAAGA AAGGCTGCGA GCAGGTCCTC TGGCTGTACG GGCCGGACCA CCAGCTCACC 
GAGGTGGGAA CCATGAACAT CTTTGTCTAC TGGACTCATG AAGACGGAGA ACTGGAGCTG 
GCAACCCCCC CACTGGATGG CATCATCCTC CCCGGAGTGG TTCGACAAAG CCTGCTGGAT 
CTGGCCAGGA CTTGGGGTGA GTTCCGGGTG GCAGAACGCA AGGTCACTAT GAAGGAATTG 
AAGCGGGCAC TGGAGGAGGG CCGGGTGCGA GAAGTCTTCG GCTCAGGCAC TGCTTGTCAG 
GTCTGCCCAG TGCACCAAAT CCTATATGAA GGCAAGCAAC TCCACATTCC TACCATGGAA 
AATGGGCCGG AACTCATCCT GCGCTTCCAG AAGGAGTTGA AGGCTATTCA GTACGGAACC 
AGTGCCCACG ATTGGATGCT TCGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcat2 ( XM_008759413.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757635.1 Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43571
Clone ID Related Accession (Same CDS sequence)	XM_008759413.1
Accession Version	XM_008759413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CTATACTAGG ACAGGTCTGG GCCCGGAAGC TTCTGCCTAT TCCCTGGCGT 
CTATGTGTGC CCGGAAGATG TGTGTCCTCC AACTTCAAGG CGGCAGACCT CCAGGTTCAG 
GTGACCAGAG AGCCACAGAA GAAGCCAGCC CCTAGCCAGC CTCTGCTGTT TGGGAAGACA 
TTCACGGACC ACATGCTGAT GGTGGAGTGG AACAGCAAGA CAGGCTGGGG CCCTCCAAGG 
ATCCAGCCCT TCCAGAACCT CACACTGCAC CCAGCCTGCT CCGGCCTGCA CTACTCATTG 
CAGCTCTTTG AAGGCTTGAA AGCTTATAAA GGCAGAGACA AGCAGGTACG CCTCTTCCGG 
CCCTGGCTCA ACATGGACAG GATGCTACGT TCTGCAAGGC GCCTCTGCCT GCCAGAACTG 
CTGGAGTGCA TCCGCCAGCT CATTGAAGTA GACAAAGACT GGGTTCCCGA TGGCAATGGA 
ACAAGCCTCT ATGTGCGGCC GGTGCTTATT GGAAATGAGC CCTCCCTGGG TGTTGGAATG 
GTCACACAAG CCCTCCTGTT CGTCATTCTG TGCCCTGTGG GCTCCTACTT CCCCGGAGAC 
TCCATGACAC CTGTCTCCCT CCTGGCGGAC CCCTCATTCG TCAGAGCCTG GATTGGTGGG 
GTCGGTGACT GCAAGTTGGG GGGGAATTAC GGCCCCACTG TGGCTGTGCA ACAAGAAGCC 
CAGAAGAAAG GCTGCGAGCA GGTCCTCTGG CTGTACGGGC CGGACCACCA GCTCACCGAG 
GTGGGAACCA TGAACATCTT TGTCTACTGG ACTCATGAAG ACGGAGAACT GGAGCTGGCA 
ACCCCCCCAC TGGATGGCAT CATCCTCCCC GGAGTGGTTC GACAAAGCCT GCTGGATCTG 
GCCAGGACTT GGGGTGAGTT CCGGGTGGCA GAACGCAAGG TCACTATGAA GGAATTGAAG 
CGGGCACTGG AGGAGGGCCG GGTGCGAGAA GTCTTCGGCT CAGGCACTGC TTGTCAGGTC 
TGCCCAGTGC ACCAAATCCT ATATGAAGGC AAGCAACTCC ACATTCCTAC CATGGAAAAT 
GGGCCGGAAC TCATCCTGCG CTTCCAGAAG GAGTTGAAGG CTATTCAGTA CGGAACCAGT 
GCCCACGATT GGATGCTTCG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757635.1
CDS	750..1916
Translation	MAAAILGQVWARKLLPIPWRLCVPGRCVSSNFKAADLQVQVTREPQKKPAPSQPLLFGKTFTDHMLMVEWNSKTGWGPPRIQPFQNLTLHPACSGLHYSLQLFEGLKAYKGRDKQVRLFRPWLNMDRMLRSARRLCLPELLECIRQLIEVDKDWVPDGNGTSLYVRPVLIGNEPSLGVGMVTQALLFVILCPVGSYFPGDSMTPVSLLADPSFVRAWIGGVGDCKLGGNYGPTVAVQQEAQKKGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGELELATPPLDGIILPGVVRQSLLDLARTWGEFRVAERKVTMKELKRALEEGRVREVFGSGTACQVCPVHQILYEGKQLHIPTMENGPELILRFQKELKAIQYGTSAHDWMLRV

Target ORF information:

RefSeq Version	XM_008759413.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus branched chain amino acid transaminase 2 (Bcat2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759413.1

ORF Insert Sequence:

ATGGCTGCAG CTATACTAGG ACAGGTCTGG GCCCGGAAGC TTCTGCCTAT TCCCTGGCGT 
CTATGTGTGC CCGGAAGATG TGTGTCCTCC AACTTCAAGG CGGCAGACCT CCAGGTTCAG 
GTGACCAGAG AGCCACAGAA GAAGCCAGCC CCTAGCCAGC CTCTGCTGTT TGGGAAGACA 
TTCACGGACC ACATGCTGAT GGTGGAGTGG AACAGCAAGA CAGGCTGGGG CCCTCCAAGG 
ATCCAGCCCT TCCAGAACCT CACACTGCAC CCAGCCTGCT CCGGCCTGCA CTACTCATTG 
CAGCTCTTTG AAGGCTTGAA AGCTTATAAA GGCAGAGACA AGCAGGTACG CCTCTTCCGG 
CCCTGGCTCA ACATGGACAG GATGCTACGT TCTGCAAGGC GCCTCTGCCT GCCAGAACTG 
CTGGAGTGCA TCCGCCAGCT CATTGAAGTA GACAAAGACT GGGTTCCCGA TGGCAATGGA 
ACAAGCCTCT ATGTGCGGCC GGTGCTTATT GGAAATGAGC CCTCCCTGGG TGTTGGAATG 
GTCACACAAG CCCTCCTGTT CGTCATTCTG TGCCCTGTGG GCTCCTACTT CCCCGGAGAC 
TCCATGACAC CTGTCTCCCT CCTGGCGGAC CCCTCATTCG TCAGAGCCTG GATTGGTGGG 
GTCGGTGACT GCAAGTTGGG GGGGAATTAC GGCCCCACTG TGGCTGTGCA ACAAGAAGCC 
CAGAAGAAAG GCTGCGAGCA GGTCCTCTGG CTGTACGGGC CGGACCACCA GCTCACCGAG 
GTGGGAACCA TGAACATCTT TGTCTACTGG ACTCATGAAG ACGGAGAACT GGAGCTGGCA 
ACCCCCCCAC TGGATGGCAT CATCCTCCCC GGAGTGGTTC GACAAAGCCT GCTGGATCTG 
GCCAGGACTT GGGGTGAGTT CCGGGTGGCA GAACGCAAGG TCACTATGAA GGAATTGAAG 
CGGGCACTGG AGGAGGGCCG GGTGCGAGAA GTCTTCGGCT CAGGCACTGC TTGTCAGGTC 
TGCCCAGTGC ACCAAATCCT ATATGAAGGC AAGCAACTCC ACATTCCTAC CATGGAAAAT 
GGGCCGGAAC TCATCCTGCG CTTCCAGAAG GAGTTGAAGG CTATTCAGTA CGGAACCAGT 
GCCCACGATT GGATGCTTCG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ketoisocaproic acid, a metabolite of leucine, suppresses insulin-stimulated glucose transport in skeletal muscle cells in a BCAT2-dependent manner.
American journal of physiology. Cell physiology311(3)C518-27(2016 09)
Moghei M,Tavajohi-Fini P,Beatty B,Adegoke OA

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)
Tarantino C,Paolella G,Cozzuto L,Minopoli G,Pastore L,Parisi S,Russo T

Alcohol-induced IGF-I resistance is ameliorated in mice deficient for mitochondrial branched-chain aminotransferase.
The Journal of nutrition140(5)932-8(2010 May)
Lang CH,Lynch CJ,Vary TC

Branched-chain amino acid metabolon: interaction of glutamate dehydrogenase with the mitochondrial branched-chain aminotransferase (BCATm).
The Journal of biological chemistry285(1)265-76(2010 Jan)
Islam MM,Nautiyal M,Wynn RM,Mobley JA,Chuang DT,Hutson SM

Disruption of BCATm in mice leads to increased energy expenditure associated with the activation of a futile protein turnover cycle.
Cell metabolism6(3)181-94(2007 Sep)
She P,Reid TM,Bronson SK,Vary TC,Hajnal A,Lynch CJ,Hutson SM

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Bcat2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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