Btc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Btc
Entrez Gene ID	64022
Full Name	betacellulin
General protein information	Preferred Names betacellulin Names probetacellulin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	may have mitogenic activity; mouse homolog induces differentiation of pancreatic beta-cells and promotes regeneration of beta-cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022256.2	NP_071592.1	probetacellulin precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC482682	XP_005629452.1
Rattus norvegicus (Norway rat)	Btc	NP_071592.1
Danio rerio (zebrafish)	zgc:153253	NP_001154295.1
Homo sapiens (human)	BTC	NP_001720.1
Pan troglodytes (chimpanzee)	BTC	XP_517223.2
Macaca mulatta (Rhesus monkey)	BTC	XP_001101880.1
Mus musculus (house mouse)	Btc	NP_031594.1
Danio rerio (zebrafish)	btc	NP_001038229.2
Gallus gallus (chicken)	BTC	NP_001004769.1
Bos taurus (cattle)	BTC	NP_776321.2
Xenopus tropicalis (tropical clawed frog)	LOC100497610	XP_002941813.1

Gene Ontology
Bibliography

Related articles in PubMed

Nerve growth factor neutralization suppresses β-cell proliferation through activin A and betacellulin.
Gezginci-Oktayoglu S, Karatug A, Bolkent S
Pancreas44(2)243-9(2015 Mar)

Gene therapy with neurogenin 3 and betacellulin reverses major metabolic problems in insulin-deficient diabetic mice.
Yechoor V, Liu V, Paul A, Lee J, Buras E, Ozer K, Samson S, Chan L
Endocrinology150(11)4863-73(2009 Nov)

Betacellulin stimulates growth of the mouse intestinal epithelium and increases adenoma multiplicity in Apc+/Min mice.
Dahlhoff M, Horst D, Gerhard M, Kolligs FT, Wolf E, Schneider MR
FEBS letters582(19)2911-5(2008 Aug)

Coexpression of Pdx1 and betacellulin in mesenchymal stem cells could promote the differentiation of nestin-positive epithelium-like progenitors and pancreatic islet-like spheroids.
Li L, Li F, Qi H, Feng G, Yuan K, Deng H, Zhou H
Stem cells and development17(4)815-23(2008 Aug)

Remission of diabetes by beta-cell regeneration in diabetic mice treated with a recombinant adenovirus expressing betacellulin.
Shin S, Li N, Kobayashi N, Yoon JW, Jun HS
Molecular therapy : the journal of the American Society of Gene Therapy16(5)854-61(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Btc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Btc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05559	NM_022256.2 Latest version!	Rattus norvegicus betacellulin (Btc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Btc ( NM_022256.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071592.1 Rattus norvegicus betacellulin (Btc), mRNA.

Price & Availability↑

CloneID	ORa05559
Clone ID Related Accession (Same CDS sequence)	NM_022256.2
Accession Version	NM_022256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527264000000
Organism	Rattus norvegicus(Norway rat)
Product	probetacellulin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB028862.1. On Feb 21, 2013 this sequence version replaced NM_022256.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB028862.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCGA CTGCCCCAGG CAGCGGTGTC AGCTCCCTGC CGCTGCTCCT GGCCCTTGTC 
CTGGGTCTTG TGATTCTCCA GTGCGTGGTG GCAGATGGGA ACACAACCAG AACCCCTGAA 
ACCAATGGCT CTCTTTGTGG AGCTCCTGGG GAAAACTGCA CAGGTACCAC CCCTAGACAG 
AAATCGAAAA CCCACTTCTC TCGGTGCCCC AAGCAATACA AGCATTACTG CATCCATGGG 
AGATGCCGCT TCGTGATGGA CGAACAAACT CCCTCCTGCA TCTGTGAGAA AGGCTACTTT 
GGGGCCCGGT GTGAGCAGGT GGACCTGTTT TATCTCCAGC AGGACAGGGG GCAGATCCTG 
GTGGTCTGCT TGATAGGCGT CATGGTGCTG TTCATCATTT TAGTCATTGG CGTCTGCACC 
TGCTGTCATC CTCTTCGGAA ACATCGCAAA AAGAAGAAGG AAGAGAAAAT GGAAACTTTG 
AGTAAAGATA AAACTCCCAT AAGTGAAGAT ATTCAAGAGA CCAATATTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071592.1
CDS	49..582
Translation	MDSTAPGSGVSSLPLLLALVLGLVILQCVVADGNTTRTPETNGSLCGAPGENCTGTTPRQKSKTHFSRCPKQYKHYCIHGRCRFVMDEQTPSCICEKGYFGARCEQVDLFYLQQDRGQILVVCLIGVMVLFIILVIGVCTCCHPLRKHRKKKKEEKMETLSKDKTPISEDIQETNIA

Target ORF information:

RefSeq Version	NM_022256.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus betacellulin (Btc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022256.2

ORF Insert Sequence:

ATGGACTCGA CTGCCCCAGG CAGCGGTGTC AGCTCCCTGC CGCTGCTCCT GGCCCTTGTC 
CTGGGTCTTG TGATTCTCCA GTGCGTGGTG GCAGATGGGA ACACAACCAG AACCCCTGAA 
ACCAATGGCT CTCTTTGTGG AGCTCCTGGG GAAAACTGCA CAGGTACCAC CCCTAGACAG 
AAATCGAAAA CCCACTTCTC TCGGTGCCCC AAGCAATACA AGCATTACTG CATCCATGGG 
AGATGCCGCT TCGTGATGGA CGAACAAACT CCCTCCTGCA TCTGTGAGAA AGGCTACTTT 
GGGGCCCGGT GTGAGCAGGT GGACCTGTTT TATCTCCAGC AGGACAGGGG GCAGATCCTG 
GTGGTCTGCT TGATAGGCGT CATGGTGCTG TTCATCATTT TAGTCATTGG CGTCTGCACC 
TGCTGTCATC CTCTTCGGAA ACATCGCAAA AAGAAGAAGG AAGAGAAAAT GGAAACTTTG 
AGTAAAGATA AAACTCCCAT AAGTGAAGAT ATTCAAGAGA CCAATATTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nerve growth factor neutralization suppresses β-cell proliferation through activin A and betacellulin.
Pancreas44(2)243-9(2015 Mar)
Gezginci-Oktayoglu S,Karatug A,Bolkent S

Gene therapy with neurogenin 3 and betacellulin reverses major metabolic problems in insulin-deficient diabetic mice.
Endocrinology150(11)4863-73(2009 Nov)
Yechoor V,Liu V,Paul A,Lee J,Buras E,Ozer K,Samson S,Chan L

Betacellulin stimulates growth of the mouse intestinal epithelium and increases adenoma multiplicity in Apc+/Min mice.
FEBS letters582(19)2911-5(2008 Aug)
Dahlhoff M,Horst D,Gerhard M,Kolligs FT,Wolf E,Schneider MR

Coexpression of Pdx1 and betacellulin in mesenchymal stem cells could promote the differentiation of nestin-positive epithelium-like progenitors and pancreatic islet-like spheroids.
Stem cells and development17(4)815-23(2008 Aug)
Li L,Li F,Qi H,Feng G,Yuan K,Deng H,Zhou H

Remission of diabetes by beta-cell regeneration in diabetic mice treated with a recombinant adenovirus expressing betacellulin.
Molecular therapy : the journal of the American Society of Gene Therapy16(5)854-61(2008 May)
Shin S,Li N,Kobayashi N,Yoon JW,Jun HS