Unc5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Unc5a
Entrez Gene ID	60629
Full Name	unc-5 netrin receptor A
Synonyms	Unc5h1
General protein information	Preferred Names unc-5 netrin receptor A Names netrin receptor UNC5A protein unc-5 homolog 1 protein unc-5 homolog A transmembrane receptor Unc5H1 unc-5 homolog 1 unc-5 homolog A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	a netrin receptor involved in neuronal migration [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022206.1	NP_071542.1	netrin receptor UNC5A precursor
XM_008771505.1	XP_008769727.1	netrin receptor UNC5A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	UNC5A	NP_588610.2
Canis lupus familiaris (dog)	UNC5A	XP_005619233.1
Danio rerio (zebrafish)	unc5a	NP_001107937.1
Xenopus tropicalis (tropical clawed frog)	unc5a	NP_001093674.2
Pan troglodytes (chimpanzee)	UNC5A	XP_518123.3
Bos taurus (cattle)	UNC5A	XP_005209218.1
Mus musculus (house mouse)	Unc5a	NP_694771.1
Rattus norvegicus (Norway rat)	Unc5a	NP_071542.1
Gallus gallus (chicken)	UNC5A	XP_424067.4

Gene Ontology
Bibliography

Related articles in PubMed

Schwann cells direct peripheral nerve regeneration through the Netrin-1 receptors, DCC and Unc5H2.
Webber CA, Christie KJ, Cheng C, Martinez JA, Singh B, Singh V, Thomas D, Zochodne DW
Glia59(10)1503-17(2011 Oct)

Peri-pubertal emergence of UNC-5 homologue expression by dopamine neurons in rodents.
Manitt C, Labelle-Dumais C, Eng C, Grant A, Mimee A, Stroh T, Flores C
PloS one5(7)e11463(2010 Jul)

The ratio of 'deleted in colorectal cancer' to 'uncoordinated-5A' netrin-1 receptors on the growth cone regulates mossy fibre directionality.
Muramatsu R, Nakahara S, Ichikawa J, Watanabe K, Matsuki N, Koyama R
Brain : a journal of neurology133(Pt 1)60-75(2010 Jan)

Spatial and temporal activation of the small GTPases RhoA and Rac1 by the netrin-1 receptor UNC5a during neurite outgrowth.
Picard M, Petrie RJ, Antoine-Bertrand J, Saint-Cyr-Proulx E, Villemure JF, Lamarche-Vane N
Cellular signalling21(12)1961-73(2009 Dec)

Topography of thalamic projections requires attractive and repulsive functions of Netrin-1 in the ventral telencephalon.
Powell AW, Sassa T, Wu Y, Tessier-Lavigne M, Polleux F
PLoS biology6(5)e116(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Unc5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Unc5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05429	NM_022206.1 Latest version!	Rattus norvegicus unc-5 netrin receptor A (Unc5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa43532	XM_008771505.1 Latest version!	Rattus norvegicus unc-5 netrin receptor A (Unc5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Unc5a ( NM_022206.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071542.1 Rattus norvegicus unc-5 netrin receptor A (Unc5a), mRNA.

Price & Availability↑

CloneID	ORa05429
Clone ID Related Accession (Same CDS sequence)	NM_022206.1
Accession Version	NM_022206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Rattus norvegicus(Norway rat)
Product	netrin receptor UNC5A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U87305.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U87305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGTCC GGCCCGGCCT GTGGCCAGTG CTCCTGGGCA TAGTCCTCGC CGCCTGGCTT 
CGTGGTTCGG GTGCCCAGCA GAGTGCCACG GTGGCCAATC CAGTGCCCGG TGCCAACCCC 
GACCTGCTGC CCCACTTCCT GGTAGAGCCT GAGGACGTGT ACATTGTCAA GAACAAGCCG 
GTGTTGTTGG TGTGCAAGGC TGTGCCTGCC ACCCAGATCT TCTTCAAGTG CAATGGGGAA 
TGGGTCCGCC AGGTCGATCA CGTAATTGAA CGCAGCACCG ACAGCAGCAG CGGATTGCCA 
ACCATGGAGG TCCGTATCAA CGTATCGAGG CAGCAGGTAG AGAAAGTGTT TGGGCTGGAG 
GAATACTGGT GCCAGTGTGT GGCATGGAGC TCCTCGGGTA CCACCAAAAG TCAGAAGGCC 
TACATCCGGA TTGCCTATTT GCGCAAGAAC TTTGAGCAGG AGCCACTGGC CAAGGAAGTG 
TCACTGGAGC AAGGCATTGT ACTACCTTGT CGCCCCCCAG AAGGAATCCC CCCAGCTGAG 
GTGGAGTGGC TTCGAAATGA GGACCTCGTG GACCCCTCCC TCGATCCCAA TGTGTACATC 
ACGCGGGAGC ACAGCCTAGT CGTGCGTCAG GCCCGCCTGG CCGACACGGC CAACTACACC 
TGTGTGGCCA AGAACATCGT AGCCCGTCGC CGAAGCACCT CTGCAGCGGT CATTGTTTAT 
GTGAACGGTG GGTGGTCGAC GTGGACTGAG TGGTCCGTCT GCAGCGCCAG CTGTGGGCGT 
GGCTGGCAGA AACGGAGCCG GAGCTGCACC AACCCGGCAC CTCTCAACGG GGGCGCCTTC 
TGTGAGGGGC AGAATGTCCA GAAAACAGCC TGCGCCACTC TGTGCCCAGT GGATGGGAGC 
TGGAGTTCGT GGAGTAAGTG GTCAGCCTGT GGGCTTGACT GCACCCACTG GCGGAGCCGC 
GAGTGCTCTG ACCCAGCACC CCGCAATGGA GGTGAGGAGT GTCGGGGTGC TGACCTGGAC 
ACCCGCAACT GTACCAGTGA CCTCTGCCTG CACACCGCTT CTTGCCCCGA GGACGTGGCT 
CTCTACATCG GCCTTGTCGC TGTGGCTGTG TGCCTCTTCT TGCTGTTGCT GGCCCTTGGA 
CTCATTTACT GTCGCAAGAA GGAAGGGCTG GACTCCGATG TGGCCGACTC GTCCATCCTC 
ACCTCGGGCT TCCAGCCTGT CAGCATCAAG CCCAGCAAAG CAGACAACCC CCACCTGCTC 
ACCATCCAGC CAGACCTCAG CACCACCACT ACCACCTACC AGGGCAGTCT ATGTTCGAGG 
CAGGATGGAC CCAGCCCCAA GTTCCAGCTC TCTAATGGTC ACCTGCTCAG CCCACTGGGG 
AGTGGCCGCC ATACGTTGCA CCACAGCTCA CCCACCTCTG AGGCTGAGGA CTTCGTCTCC 
CGCCTCTCCA CCCAAAACTA CTTTCGTTCC CTGCCCCGCG GCACCAGCAA CATGGCCTAC 
GGGACCTTCA ACTTCCTCGG GGGCCGGCTG ATGATCCCTA ATACGGGGAT CAGCCTCCTC 
ATACCCCCGG ATGCCATCCC CCGAGGAAAG ATCTACGAGA TCTACCTCAC ACTGCACAAG 
CCAGAAGACG TGAGGTTGCC CCTAGCTGGC TGTCAGACCC TGCTGAGTCC AGTCGTTAGC 
TGTGGGCCCC CAGGAGTCCT GCTCACCCGG CCAGTCATCC TTGCAATGGA CCACTGTGGA 
GAGCCCAGCC CTGACAGCTG GAGTCTGCGC CTCAAAAAGC AGTCCTGCGA GGGCAGTTGG 
GAGGATGTGC TGCACCTTGG TGAGGAGTCA CCTTCCCACC TCTACTACTG CCAGCTGGAG 
GCCGGGGCCT GCTATGTCTT CACGGAGCAG CTGGGCCGCT TTGCCCTGGT AGGAGAGGCC 
CTCAGCGTGG CTGCCACCAA GCGCCTCAGG CTCCTTCTGT TTGCTCCCGT GGCCTGTACG 
TCCCTTGAGT ACAACATCCG AGTGTACTGC CTACACGACA CCCACGACGC TCTCAAGGAG 
GTGGTGCAGC TGGAGAAGCA GCTAGGTGGA CAGCTGATCC AGGAGCCTCG CGTCCTGCAC 
TTCAAAGACA GTTACCACAA CCTACGTCTC TCCATCCACG ACGTGCCCAG CTCCCTGTGG 
AAGAGCAAGC TACTTGTCAG CTACCAGGAG ATCCCTTTTT ACCACATCTG GAACGGCACC 
CAGCAGTATC TGCACTGCAC CTTCACCCTG GAGCGCATCA ACGCCAGCAC CAGCGACCTG 
GCCTGCAAGG TGTGGGTGTG GCAGGTGGAG GGAGATGGGC AGAGCTTCAA CATCAACTTC 
AACATCACTA AGGACACAAG GTTTGCTGAA TTGTTGGCTC TGGAGAGTGA AGGGGGGGTC 
CCAGCCCTGG TGGGCCCCAG TGCCTTCAAG ATCCCCTTCC TCATTCGGCA AAAGATCATC 
GCCAGTCTGG ACCCACCCTG CAGCCGGGGC GCCGACTGGA GAACTCTAGC CCAGAAACTT 
CACCTGGACA GCCATCTTAG CTTCTTTGCC TCCAAGCCCA GCCCTACAGC CATGATCCTC 
AACCTATGGG AGGCACGGCA CTTCCCCAAC GGCAACCTCG GCCAGCTGGC AGCAGCTGTG 
GCCGGACTGG GCCAACCAGA TGCTGGCCTC TTCACGGTGT CGGAGGCCGA GTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071542.1
CDS	1..2697
Translation	MAVRPGLWPVLLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDSSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIAYLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSTSAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVQKTACATLCPVDGSWSSWSKWSACGLDCTHWRSRECSDPAPRNGGEECRGADLDTRNCTSDLCLHTASCPEDVALYIGLVAVAVCLFLLLLALGLIYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTTYQGSLCSRQDGPSPKFQLSNGHLLSPLGSGRHTLHHSSPTSEAEDFVSRLSTQNYFRSLPRGTSNMAYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPVVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEDVLHLGEESPSHLYYCQLEAGACYVFTEQLGRFALVGEALSVAATKRLRLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQQYLHCTFTLERINASTSDLACKVWVWQVEGDGQSFNINFNITKDTRFAELLALESEGGVPALVGPSAFKIPFLIRQKIIASLDPPCSRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLGQLAAAVAGLGQPDAGLFTVSEAEC

Target ORF information:

RefSeq Version	NM_022206.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus unc-5 netrin receptor A (Unc5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022206.1

ORF Insert Sequence:

ATGGCCGTCC GGCCCGGCCT GTGGCCAGTG CTCCTGGGCA TAGTCCTCGC CGCCTGGCTT 
CGTGGTTCGG GTGCCCAGCA GAGTGCCACG GTGGCCAATC CAGTGCCCGG TGCCAACCCC 
GACCTGCTGC CCCACTTCCT GGTAGAGCCT GAGGACGTGT ACATTGTCAA GAACAAGCCG 
GTGTTGTTGG TGTGCAAGGC TGTGCCTGCC ACCCAGATCT TCTTCAAGTG CAATGGGGAA 
TGGGTCCGCC AGGTCGATCA CGTAATTGAA CGCAGCACCG ACAGCAGCAG CGGATTGCCA 
ACCATGGAGG TCCGTATCAA CGTATCGAGG CAGCAGGTAG AGAAAGTGTT TGGGCTGGAG 
GAATACTGGT GCCAGTGTGT GGCATGGAGC TCCTCGGGTA CCACCAAAAG TCAGAAGGCC 
TACATCCGGA TTGCCTATTT GCGCAAGAAC TTTGAGCAGG AGCCACTGGC CAAGGAAGTG 
TCACTGGAGC AAGGCATTGT ACTACCTTGT CGCCCCCCAG AAGGAATCCC CCCAGCTGAG 
GTGGAGTGGC TTCGAAATGA GGACCTCGTG GACCCCTCCC TCGATCCCAA TGTGTACATC 
ACGCGGGAGC ACAGCCTAGT CGTGCGTCAG GCCCGCCTGG CCGACACGGC CAACTACACC 
TGTGTGGCCA AGAACATCGT AGCCCGTCGC CGAAGCACCT CTGCAGCGGT CATTGTTTAT 
GTGAACGGTG GGTGGTCGAC GTGGACTGAG TGGTCCGTCT GCAGCGCCAG CTGTGGGCGT 
GGCTGGCAGA AACGGAGCCG GAGCTGCACC AACCCGGCAC CTCTCAACGG GGGCGCCTTC 
TGTGAGGGGC AGAATGTCCA GAAAACAGCC TGCGCCACTC TGTGCCCAGT GGATGGGAGC 
TGGAGTTCGT GGAGTAAGTG GTCAGCCTGT GGGCTTGACT GCACCCACTG GCGGAGCCGC 
GAGTGCTCTG ACCCAGCACC CCGCAATGGA GGTGAGGAGT GTCGGGGTGC TGACCTGGAC 
ACCCGCAACT GTACCAGTGA CCTCTGCCTG CACACCGCTT CTTGCCCCGA GGACGTGGCT 
CTCTACATCG GCCTTGTCGC TGTGGCTGTG TGCCTCTTCT TGCTGTTGCT GGCCCTTGGA 
CTCATTTACT GTCGCAAGAA GGAAGGGCTG GACTCCGATG TGGCCGACTC GTCCATCCTC 
ACCTCGGGCT TCCAGCCTGT CAGCATCAAG CCCAGCAAAG CAGACAACCC CCACCTGCTC 
ACCATCCAGC CAGACCTCAG CACCACCACT ACCACCTACC AGGGCAGTCT ATGTTCGAGG 
CAGGATGGAC CCAGCCCCAA GTTCCAGCTC TCTAATGGTC ACCTGCTCAG CCCACTGGGG 
AGTGGCCGCC ATACGTTGCA CCACAGCTCA CCCACCTCTG AGGCTGAGGA CTTCGTCTCC 
CGCCTCTCCA CCCAAAACTA CTTTCGTTCC CTGCCCCGCG GCACCAGCAA CATGGCCTAC 
GGGACCTTCA ACTTCCTCGG GGGCCGGCTG ATGATCCCTA ATACGGGGAT CAGCCTCCTC 
ATACCCCCGG ATGCCATCCC CCGAGGAAAG ATCTACGAGA TCTACCTCAC ACTGCACAAG 
CCAGAAGACG TGAGGTTGCC CCTAGCTGGC TGTCAGACCC TGCTGAGTCC AGTCGTTAGC 
TGTGGGCCCC CAGGAGTCCT GCTCACCCGG CCAGTCATCC TTGCAATGGA CCACTGTGGA 
GAGCCCAGCC CTGACAGCTG GAGTCTGCGC CTCAAAAAGC AGTCCTGCGA GGGCAGTTGG 
GAGGATGTGC TGCACCTTGG TGAGGAGTCA CCTTCCCACC TCTACTACTG CCAGCTGGAG 
GCCGGGGCCT GCTATGTCTT CACGGAGCAG CTGGGCCGCT TTGCCCTGGT AGGAGAGGCC 
CTCAGCGTGG CTGCCACCAA GCGCCTCAGG CTCCTTCTGT TTGCTCCCGT GGCCTGTACG 
TCCCTTGAGT ACAACATCCG AGTGTACTGC CTACACGACA CCCACGACGC TCTCAAGGAG 
GTGGTGCAGC TGGAGAAGCA GCTAGGTGGA CAGCTGATCC AGGAGCCTCG CGTCCTGCAC 
TTCAAAGACA GTTACCACAA CCTACGTCTC TCCATCCACG ACGTGCCCAG CTCCCTGTGG 
AAGAGCAAGC TACTTGTCAG CTACCAGGAG ATCCCTTTTT ACCACATCTG GAACGGCACC 
CAGCAGTATC TGCACTGCAC CTTCACCCTG GAGCGCATCA ACGCCAGCAC CAGCGACCTG 
GCCTGCAAGG TGTGGGTGTG GCAGGTGGAG GGAGATGGGC AGAGCTTCAA CATCAACTTC 
AACATCACTA AGGACACAAG GTTTGCTGAA TTGTTGGCTC TGGAGAGTGA AGGGGGGGTC 
CCAGCCCTGG TGGGCCCCAG TGCCTTCAAG ATCCCCTTCC TCATTCGGCA AAAGATCATC 
GCCAGTCTGG ACCCACCCTG CAGCCGGGGC GCCGACTGGA GAACTCTAGC CCAGAAACTT 
CACCTGGACA GCCATCTTAG CTTCTTTGCC TCCAAGCCCA GCCCTACAGC CATGATCCTC 
AACCTATGGG AGGCACGGCA CTTCCCCAAC GGCAACCTCG GCCAGCTGGC AGCAGCTGTG 
GCCGGACTGG GCCAACCAGA TGCTGGCCTC TTCACGGTGT CGGAGGCCGA GTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc5a ( XM_008771505.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769727.1 Rattus norvegicus unc-5 netrin receptor A (Unc5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43532
Clone ID Related Accession (Same CDS sequence)	XM_008771505.1
Accession Version	XM_008771505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	netrin receptor UNC5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTCC GGCCCGGCCT GTGGCCAGTG CTCCTGGGCA TAGTCCTCGC CGCCTGGCTT 
CGTGGTTCGG GTGCCCAGCA GAGTGCCACG GTGGCCAATC CAGTGCCCGG TGCCAACCCC 
GACCTGCTGC CCCACTTCCT GGTAGAGCCT GAGGACGTGT ACATTGTCAA GAACAAGCCG 
GTGTTGTTGG TGTGCAAGGC TGTGCCTGCC ACCCAGATCT TCTTCAAGTG CAATGGGGAA 
TGGGTCCGCC AGGTCGATCA CGTAATTGAA CGCAGCACCG ACAGCAGCAG CGGATTGCCA 
ACCATGGAGG TCCGTATCAA CGTATCGAGG CAGCAGGTAG AGAAAGTGTT TGGGCTGGAG 
GAATACTGGT GCCAGTGTGT GGCATGGAGC TCCTCGGGTA CCACCAAAAG TCAGAAGGCC 
TACATCCGGA TTGCCTATTT GCGCAAGAAC TTTGAGCAGG AGCCACTGGC CAAGGAAGTG 
TCACTGGAGC AAGGCATTGT ACTACCTTGT CGCCCCCCAG AAGGAATCCC CCCAGCTGAG 
GTGGAGTGGC TTCGAAATGA GGACCTCGTG GACCCCTCCC TCGATCCCAA TGTGTACATC 
ACGCGGGAGC ACAGCCTAGT CGTGCGTCAG GCCCGCCTGG CCGACACGGC CAACTACACC 
TGTGTGGCCA AGAACATCGT AGCCCGTCGC CGAAGCACCT CTGCAGCGGT CATTGTTTAT 
GTGGATGGGA GCTGGAGTTC GTGGAGTAAG TGGTCAGCCT GTGGGCTTGA CTGCACCCAC 
TGGCGGAGCC GCGAGTGCTC TGACCCAGCA CCCCGCAATG GAGGTGAGGA GTGTCGGGGT 
GCTGACCTGG ACACCCGCAA CTGTACCAGT GACCTCTGCC TGCACACCGC TTCTGGCCCC 
GAGGACGTGG CTCTCTACAT CGGCCTTGTC GCTGTGGCTG TGTGCCTCTT CTTGCTGTTG 
CTGGCCCTTG GCCTCATTTA CTGTCGCAAG AAGGAAGGGC TGGACTCCGA TGTGGCCGAC 
TCGTCCATCC TCACCTCGGG CTTCCAGCCT GTCAGCATCA AGCCCAGCAA AGCAGACAAC 
CCCCACCTGC TCACCATCCA GCCAGACCTC AGCACCACCA CTACCACCTA CCAGGGCAGT 
CTATGTCCGA GGCAGGATGG ACCCAGCCCC AAGTTCCAGC TCTCTAATGG TCACCTGCTC 
AGCCCACTGG GGAGTGGCCG CCATACGTTG CACCACAGCT CACCCACCTC TGAGGCTGAG 
GACTTCGTCT CCCGCCTCTC CACCCAAAAC TACTTTCGTT CCCTGCCCCG CGGCACCAGC 
AACATGGCCT ACGGGACCTT CAACTTCCTC GGGGGCCGGC TGATGATCCC TAATACGGGG 
ATCAGCCTCC TCATACCCCC GGATGCCATC CCCCGAGGAA AGATCTACGA GATCTACCTC 
ACACTGCACA AGCCAGAAGA CGTGAGGTTG CCCCTAGCTG GCTGTCAGAC CCTGCTGAGT 
CCAGTCGTTA GCTGTGGGCC CCCAGGAGTC CTGCTCACCC GGCCAGTCAT CCTTGCAATG 
GACCACTGTG GAGAGCCCAG CCCTGACAGC TGGAGTCTGC GCCTCAAAAA GCAGTCCTGC 
GAGGGCAGTT GGGAGGATGT GCTGCACCTT GGTGAGGAGT CACCTTCCCA CCTCTACTAC 
TGCCAGCTGG AGGCCGGGGC CTGCTATGTC TTCACGGAGC AGCTGGGCCG CTTTGCCCTG 
GTAGGAGAGG CCCTCAGCGT GGCTGCCACC AAGCGCCTCA GGCTCCTTCT GTTTGCTCCC 
GTGGCCTGTA CGTCCCTTGA GTACAACATC CGAGTGTACT GCCTACACGA CACCCACGAC 
GCTCTCAAGG AGGTGGTGCA GCTGGAGAAG CAGCTAGGTG GACAGCTGAT CCAGGAGCCT 
CGCGTCCTGC ACTTCAAAGA CAGTTACCAC AACCTACGTC TCTCCATCCA CGACGTGCCC 
AGCTCCCTGT GGAAGAGCAA GCTACTTGTC AGCTACCAGG AGATCCCTTT TTACCACATC 
TGGAACGGCA CCCAGCAGTA TCTGCACTGC ACCTTCACCC TGGAGCGCAT CAACGCCAGC 
ACCAGCGACC TGGCCTGCAA GGTGTGGGTG TGGCAGGTGG AGGGAGATGG GCAGAGCTTC 
AACATCAACT TCAACATCAC TAAGGACACA AGGTTTGCTG AATTGTTGGC TCTGGAGAGT 
GAAGGGGGGG TCCCAGCCCT GGTGGGCCCC AGTGCCTTCA AGATCCCCTT CCTCATTCGG 
CAAAAGATCA TCGCCAGTCT GGACCCACCC TGCAGCCGGG GCGCCGACTG GAGAACTCTA 
GCCCAGAAAC TTCACCTGGA CAGCCATCTT AGCTTCTTTG CCTCCAAGCC CAGCCCTACA 
GCCATGATCC TCAACCTATG GGAGGCACGG CACTTCCCCA ACGGCAACCT CGGCCAGCTG 
GCAGCAGCTG TGGCCGGACT GGGCCAACCA GATGCTGGCC TCTTCACGGT GTCGGAGGCC 
GAGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769727.1
CDS	240..2768
Translation	MAVRPGLWPVLLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDSSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIAYLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSTSAAVIVYVDGSWSSWSKWSACGLDCTHWRSRECSDPAPRNGGEECRGADLDTRNCTSDLCLHTASGPEDVALYIGLVAVAVCLFLLLLALGLIYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTTYQGSLCPRQDGPSPKFQLSNGHLLSPLGSGRHTLHHSSPTSEAEDFVSRLSTQNYFRSLPRGTSNMAYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPVVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEDVLHLGEESPSHLYYCQLEAGACYVFTEQLGRFALVGEALSVAATKRLRLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQQYLHCTFTLERINASTSDLACKVWVWQVEGDGQSFNINFNITKDTRFAELLALESEGGVPALVGPSAFKIPFLIRQKIIASLDPPCSRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLGQLAAAVAGLGQPDAGLFTVSEAEC

Target ORF information:

RefSeq Version	XM_008771505.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus unc-5 netrin receptor A (Unc5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771505.1

ORF Insert Sequence:

ATGGCCGTCC GGCCCGGCCT GTGGCCAGTG CTCCTGGGCA TAGTCCTCGC CGCCTGGCTT 
CGTGGTTCGG GTGCCCAGCA GAGTGCCACG GTGGCCAATC CAGTGCCCGG TGCCAACCCC 
GACCTGCTGC CCCACTTCCT GGTAGAGCCT GAGGACGTGT ACATTGTCAA GAACAAGCCG 
GTGTTGTTGG TGTGCAAGGC TGTGCCTGCC ACCCAGATCT TCTTCAAGTG CAATGGGGAA 
TGGGTCCGCC AGGTCGATCA CGTAATTGAA CGCAGCACCG ACAGCAGCAG CGGATTGCCA 
ACCATGGAGG TCCGTATCAA CGTATCGAGG CAGCAGGTAG AGAAAGTGTT TGGGCTGGAG 
GAATACTGGT GCCAGTGTGT GGCATGGAGC TCCTCGGGTA CCACCAAAAG TCAGAAGGCC 
TACATCCGGA TTGCCTATTT GCGCAAGAAC TTTGAGCAGG AGCCACTGGC CAAGGAAGTG 
TCACTGGAGC AAGGCATTGT ACTACCTTGT CGCCCCCCAG AAGGAATCCC CCCAGCTGAG 
GTGGAGTGGC TTCGAAATGA GGACCTCGTG GACCCCTCCC TCGATCCCAA TGTGTACATC 
ACGCGGGAGC ACAGCCTAGT CGTGCGTCAG GCCCGCCTGG CCGACACGGC CAACTACACC 
TGTGTGGCCA AGAACATCGT AGCCCGTCGC CGAAGCACCT CTGCAGCGGT CATTGTTTAT 
GTGGATGGGA GCTGGAGTTC GTGGAGTAAG TGGTCAGCCT GTGGGCTTGA CTGCACCCAC 
TGGCGGAGCC GCGAGTGCTC TGACCCAGCA CCCCGCAATG GAGGTGAGGA GTGTCGGGGT 
GCTGACCTGG ACACCCGCAA CTGTACCAGT GACCTCTGCC TGCACACCGC TTCTGGCCCC 
GAGGACGTGG CTCTCTACAT CGGCCTTGTC GCTGTGGCTG TGTGCCTCTT CTTGCTGTTG 
CTGGCCCTTG GCCTCATTTA CTGTCGCAAG AAGGAAGGGC TGGACTCCGA TGTGGCCGAC 
TCGTCCATCC TCACCTCGGG CTTCCAGCCT GTCAGCATCA AGCCCAGCAA AGCAGACAAC 
CCCCACCTGC TCACCATCCA GCCAGACCTC AGCACCACCA CTACCACCTA CCAGGGCAGT 
CTATGTCCGA GGCAGGATGG ACCCAGCCCC AAGTTCCAGC TCTCTAATGG TCACCTGCTC 
AGCCCACTGG GGAGTGGCCG CCATACGTTG CACCACAGCT CACCCACCTC TGAGGCTGAG 
GACTTCGTCT CCCGCCTCTC CACCCAAAAC TACTTTCGTT CCCTGCCCCG CGGCACCAGC 
AACATGGCCT ACGGGACCTT CAACTTCCTC GGGGGCCGGC TGATGATCCC TAATACGGGG 
ATCAGCCTCC TCATACCCCC GGATGCCATC CCCCGAGGAA AGATCTACGA GATCTACCTC 
ACACTGCACA AGCCAGAAGA CGTGAGGTTG CCCCTAGCTG GCTGTCAGAC CCTGCTGAGT 
CCAGTCGTTA GCTGTGGGCC CCCAGGAGTC CTGCTCACCC GGCCAGTCAT CCTTGCAATG 
GACCACTGTG GAGAGCCCAG CCCTGACAGC TGGAGTCTGC GCCTCAAAAA GCAGTCCTGC 
GAGGGCAGTT GGGAGGATGT GCTGCACCTT GGTGAGGAGT CACCTTCCCA CCTCTACTAC 
TGCCAGCTGG AGGCCGGGGC CTGCTATGTC TTCACGGAGC AGCTGGGCCG CTTTGCCCTG 
GTAGGAGAGG CCCTCAGCGT GGCTGCCACC AAGCGCCTCA GGCTCCTTCT GTTTGCTCCC 
GTGGCCTGTA CGTCCCTTGA GTACAACATC CGAGTGTACT GCCTACACGA CACCCACGAC 
GCTCTCAAGG AGGTGGTGCA GCTGGAGAAG CAGCTAGGTG GACAGCTGAT CCAGGAGCCT 
CGCGTCCTGC ACTTCAAAGA CAGTTACCAC AACCTACGTC TCTCCATCCA CGACGTGCCC 
AGCTCCCTGT GGAAGAGCAA GCTACTTGTC AGCTACCAGG AGATCCCTTT TTACCACATC 
TGGAACGGCA CCCAGCAGTA TCTGCACTGC ACCTTCACCC TGGAGCGCAT CAACGCCAGC 
ACCAGCGACC TGGCCTGCAA GGTGTGGGTG TGGCAGGTGG AGGGAGATGG GCAGAGCTTC 
AACATCAACT TCAACATCAC TAAGGACACA AGGTTTGCTG AATTGTTGGC TCTGGAGAGT 
GAAGGGGGGG TCCCAGCCCT GGTGGGCCCC AGTGCCTTCA AGATCCCCTT CCTCATTCGG 
CAAAAGATCA TCGCCAGTCT GGACCCACCC TGCAGCCGGG GCGCCGACTG GAGAACTCTA 
GCCCAGAAAC TTCACCTGGA CAGCCATCTT AGCTTCTTTG CCTCCAAGCC CAGCCCTACA 
GCCATGATCC TCAACCTATG GGAGGCACGG CACTTCCCCA ACGGCAACCT CGGCCAGCTG 
GCAGCAGCTG TGGCCGGACT GGGCCAACCA GATGCTGGCC TCTTCACGGT GTCGGAGGCC 
GAGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Schwann cells direct peripheral nerve regeneration through the Netrin-1 receptors, DCC and Unc5H2.
Glia59(10)1503-17(2011 Oct)
Webber CA,Christie KJ,Cheng C,Martinez JA,Singh B,Singh V,Thomas D,Zochodne DW

Peri-pubertal emergence of UNC-5 homologue expression by dopamine neurons in rodents.
PloS one5(7)e11463(2010 Jul)
Manitt C,Labelle-Dumais C,Eng C,Grant A,Mimee A,Stroh T,Flores C

The ratio of 'deleted in colorectal cancer' to 'uncoordinated-5A' netrin-1 receptors on the growth cone regulates mossy fibre directionality.
Brain : a journal of neurology133(Pt 1)60-75(2010 Jan)
Muramatsu R,Nakahara S,Ichikawa J,Watanabe K,Matsuki N,Koyama R

Spatial and temporal activation of the small GTPases RhoA and Rac1 by the netrin-1 receptor UNC5a during neurite outgrowth.
Cellular signalling21(12)1961-73(2009 Dec)
Picard M,Petrie RJ,Antoine-Bertrand J,Saint-Cyr-Proulx E,Villemure JF,Lamarche-Vane N

Topography of thalamic projections requires attractive and repulsive functions of Netrin-1 in the ventral telencephalon.
PLoS biology6(5)e116(2008 May)
Powell AW,Sassa T,Wu Y,Tessier-Lavigne M,Polleux F

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Unc5a cDNA ORF clone, Rattus norvegicus(Norway rat)

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