Adcy4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adcy4
Entrez Gene ID	54223
Full Name	adenylate cyclase 4
General protein information	Preferred Names adenylate cyclase 4 Names adenylate cyclase type 4 ATP pyrophosphate-lyase 4 adenylate cyclase type IV adenylyl cyclase 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13
Summary	plays a role in Gs alpha and G-protein beta gamma mediated signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599787.1	XP_017455276.1	adenylate cyclase type 4 isoform X1
NM_019285.2	NP_062158.2	adenylate cyclase type 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Adcy4	NP_062158.2
Xenopus tropicalis (tropical clawed frog)	adcy4	XP_004910799.1
Macaca mulatta (Rhesus monkey)	LOC714684	XP_001104563.2
Homo sapiens (human)	ADCY4	NP_001185497.1
Pan troglodytes (chimpanzee)	ADCY4	XP_509874.2
Bos taurus (cattle)	ADCY4	NP_001092678.1
Canis lupus familiaris (dog)	ADCY4	XP_005623331.1
Mus musculus (house mouse)	Adcy4	NP_536683.1

Gene Ontology
Bibliography

Related articles in PubMed

Distribution of adenylyl cyclases in the rat ovary by immunofluorescence microscopy.
Bagavandoss P, Grimshaw S
Anatomical record (Hoboken, N.J. : 2007)295(10)1717-26(2012 Oct)

Physiological roles for G protein-regulated adenylyl cyclase isoforms: insights from knockout and overexpression studies.
Sadana R, Dessauer CW
Neuro-Signals17(1)5-22(2009)

Role of protein kinase C-dependent A-kinase anchoring proteins in lysophosphatidic acid-induced cAMP signaling in human diploid fibroblasts.
Rhim JH, Jang IS, Yeo EJ, Song KY, Park SC
Aging cell5(6)451-61(2006 Dec)

Gbetagamma that interacts with adenylyl cyclase in opioid tolerance originates from a Gs protein.
Wang HY, Burns LH
Journal of neurobiology66(12)1302-10(2006 Oct)

Angiotensin II enhances adenylyl cyclase signaling via Ca2+/calmodulin. Gq-Gs cross-talk regulates collagen production in cardiac fibroblasts.
Ostrom RS, Naugle JE, Hase M, Gregorian C, Swaney JS, Insel PA, Brunton LL, Meszaros JG
The Journal of biological chemistry278(27)24461-8(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Adcy4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adcy4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04278	NM_019285.2 Latest version!	Rattus norvegicus adenylate cyclase 4 (Adcy4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa04278	XM_017599787.1 Latest version!	Rattus norvegicus adenylate cyclase 4 (Adcy4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adcy4 ( NM_019285.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062158.2 Rattus norvegicus adenylate cyclase 4 (Adcy4), mRNA.

Price & Availability↑

CloneID	ORa04278
Clone ID Related Accession (Same CDS sequence)	XM_017599787.1 , NM_019285.2
Accession Version	NM_019285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	adenylate cyclase type 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC081813.1. On Mar 8, 2007 this sequence version replaced NM_019285.1. ##Evidence-Data-START## Transcript exon combination :: BC081813.1, M80633.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCCGCC TCTTCAGTCC CCGGCCTCCT CCCAGCGAAG ATCTCTTCTA CGAGACTTAC 
TACAGCCTGA GCCAACAGTA TCCACTGCTA ATTCTGCTTC TGGTCATTGT GCTCTGCGCG 
ATCGTGGCAC TGCCCGCCGT CGCCTGGGCC AGCGGCAGGG AGCTGACCTC AGACCCAAGC 
TTCCTGACAA CTGTGCTGTG TGCTTTGGGT GGCTTCTCCC TGCTTTTGGG GCTCGCTTCC 
CGGGAGCAGC AACTGCAGCG ATGGACACGA CCTCTTTCTG GCCTCATATG GGCTGCATTG 
CTGGCTCTGG GCTATGGGTT TCTATTCACT GGGGGTGTGG TGAGCGCCTG GGATCAGGTG 
TCCTTTTTCC TCTTCATCAT TTTCACCGTG TACGCCATGC TGCCCTTGGG CATGCGGGAT 
GCTGCTGCCG CGGGGGTCAT CTCATCCCTC TCACACCTGC TGGTCCTTGG ACTGTACCTT 
GGGTGGCGGC CTGAGTCGCA GAGGGATCTG CTACCACAGT TGGCAGCGAA TGCGGTGTTG 
TTCCTGTGTG GGAACGTGGT GGGAGCATAC CACAAGGCCC TGATGGAGCG AGCATTGCGC 
GCCACGTTCC GGGAGGCTCT TAGCTCCCTG CATTCACGCC GGAGGTTGGA CACTGAGAAA 
AAGCACCAGG AGCACCTCCT CTTGTCTATC CTTCCTGCCT ACCTGGCCCG AGAGATGAAG 
GCAGAGATCA TGGCTCGGCT GCAGGCTGGA CAGAGCTCAC GGCCAGAGAA CACAAACAAC 
TTTCACAGCC TGTATGTCAA GAGGCACCAA GGAGTGAGTG TGCTGTATGC TGACATCGTG 
GGCTTCACAC GGCTGGCCAG TGAGTGTTCC CCTAAGGAGC TGGTGCTAAT GCTCAATGAA 
CTCTTCGGCA AATTCGACCA AATTGCAAAG GAGCACGAAT GCATGCGGAT CAAGATCCTG 
GGAGACTGTT ACTACTGTGT ATCCGGGCTG CCCCTCTCTC TGCCTGACCA CGCCATCAAT 
TGCGTGCGCA TGGGACTGGA CATGTGCCGG GCCATCAGGA AACTTCGGGT AGCCACCGGT 
GTGGATATCA ACATGCGTGT CGGTGTGCAC TCAGGCAGCG TCCTCTGTGG AGTCATCGGG 
CTACAGAAGT GGCAGTATGA TGTCTGGTCC CATGATGTCA CATTGGCCAA CCATATGGAA 
GCAGGCGGCG TTCCAGGACG AGTGCACATC ACAGGGGCCA CGCTGGCCCT GCTGGCAGGA 
GCTTATGCTG TGGAGAGAGC AGACATGGAG CATCGAGACC CATACCTTCG GGAGCTAGGG 
GAACCCACAT ACCTGGTCAT TGATCCTTGG GCTGAGGAGG AAGACGAGAA GGGCACCGAG 
AGAGGATTGC TGTCTTCTCT AGAGGGGCAC ACGATGCGTC CGTCACTACT GATGACTCGT 
TATCTGGAGT CTTGGGGTGC AGCCAAGCCT TTCGCCCACC TAAGCCACGT CGACAGTCCT 
GCATCCACAT CCACTCCACT CCCGGAGAAA GCCTTCAGCC CCCAGTGGAG CCTGGACCGG 
AGTCGCACCC CCCGCGGGCT ACACGATGAA CTGGACACTG GAGATGCCAA GTTCTTCCAG 
GTCATCGAAC AACTCAACTC TCAAAAACAG TGGAAACAGT CGAAGGACTT CAACCTCCTG 
ACGCTCTACT TCAGAGAGAA GGAGATGGAG AAACAGTATC GGCTGTCTGC GCTCCCCGCC 
TTCAAATACT ACGCAGCCTG CACCTTCCTG GTTTTTCTGT CCAACTTCAC AATCCAAATG 
CTGGTGACAA CCAGGCCCCC AGCTCTGGCC ACCACCTACA GCATCACCTT CCTTCTCTTC 
CTTCTCCTCC TCTTCGTCTG CTTCTCAGAG CACCTGACGA AGTGCGTCCA GAAAGGCCCC 
AAGATGTTGC ACTGGCTGCC TGCGCTGTCT GTCCTGGTGG CCACACGGCC GGGATTGCGA 
GTAGCCCTGG GCACAGCCAC CATCCTCCTG GTGTTCACTA TGGCCGTCGT CAGCCTGCTC 
TTCTTACCAG TGTCGTCAGA CTGCCCTTTC CTGGCTCCCA ATGTGTCGTC AGTGGCTTTT 
AATACTTCCT GGGAGCTGCC AGCATCCCTG CCTCTCATCA GCATCCCATA CTCCATGCAT 
TGTTGCGTGC TGGGTTTCCT TTCCTGCTCC CTTTTTCTGC ACATGAGCTT CGAACTGAAG 
CTGCTTCTGC TTCTGCTGTG GCTGGTGGCA TCTTGTTCCC TATTTCTGCA CTCCCACGCC 
TGGCTGTCCG ACTGCCTCAT TGCCCGCCTT TATCAAGGCT CATTGGGCTC CAGGCCGGGG 
GTACTGAAGG AACCGAAACT GATGGGAGCT ATCTACTTCT TCATCTTCTT CTTCACACTC 
CTCGTCCTGG CTCGGCAGAA TGAGTATTAC TGTCGCCTGG ACTTCCTATG GAAAAAGAAA 
CTGAGGCAGG AGCGAGAGGA AACTGAGACA ATGGAGAACC TGACCCGGCT CCTCCTGGAG 
AATGTACTCC CTGCACACGT GGCGCCCCAG TTCATCGGCC AGAACCGGCG CAATGAGGAC 
CTCTACCATC AGTCGTACGA ATGTGTTTGT GTCCTCTTTG CATCCATCCC AGACTTTAAG 
GAATTCTACT CGGAATCCAA CATCAACCAT GAGGGGCTAG AGTGTCTGCG GCTGCTCAAT 
GAGATCATTG CCGACTTTGA TGAGCTGCTC TCCAAGCCAA AGTTCAGTGG AGTAGAGAAG 
ATCAAAACTA TCGGCAGCAC CTACATGGCG GCCACAGGCC TAAATGCCAC CCCTGGGCAG 
GACACACAAC AGGACGCTGA GAGAAGCTGC AGCCATCTGG GCACCATGGT GGAATTTGCA 
GTGGCCCTGG GGTCTAAGCT GGGTGTCATC AATAAGCACT CGTTCAACAA CTTCCGCCTG 
CGTGTGGGGT TAAACCATGG ACCAGTCGTA GCAGGGGTGA TTGGGGCGCA GAAGCCACAA 
TATGACATCT GGGGGAATAC AGTGAACGTG GCCAGCCGCA TGGAGAGCAC AGGAGTTCTC 
GGCAAGATCC AAGTGACCGA GGAGACAGCT AGGGCTCTGC AATCCCTGGG TTACACATGC 
TACAGCCGAG GTGTCATCAA GGTCAAGGGC AAAGGGCAAC TCTGTACCTA CTTCCTGAAC 
ACAGACCTGA CACGAACCGG ATCTCCCTCA GCATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062158.2
CDS	144..3362
Translation	MARLFSPRPPPSEDLFYETYYSLSQQYPLLILLLVIVLCAIVALPAVAWASGRELTSDPSFLTTVLCALGGFSLLLGLASREQQLQRWTRPLSGLIWAALLALGYGFLFTGGVVSAWDQVSFFLFIIFTVYAMLPLGMRDAAAAGVISSLSHLLVLGLYLGWRPESQRDLLPQLAANAVLFLCGNVVGAYHKALMERALRATFREALSSLHSRRRLDTEKKHQEHLLLSILPAYLAREMKAEIMARLQAGQSSRPENTNNFHSLYVKRHQGVSVLYADIVGFTRLASECSPKELVLMLNELFGKFDQIAKEHECMRIKILGDCYYCVSGLPLSLPDHAINCVRMGLDMCRAIRKLRVATGVDINMRVGVHSGSVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHITGATLALLAGAYAVERADMEHRDPYLRELGEPTYLVIDPWAEEEDEKGTERGLLSSLEGHTMRPSLLMTRYLESWGAAKPFAHLSHVDSPASTSTPLPEKAFSPQWSLDRSRTPRGLHDELDTGDAKFFQVIEQLNSQKQWKQSKDFNLLTLYFREKEMEKQYRLSALPAFKYYAACTFLVFLSNFTIQMLVTTRPPALATTYSITFLLFLLLLFVCFSEHLTKCVQKGPKMLHWLPALSVLVATRPGLRVALGTATILLVFTMAVVSLLFLPVSSDCPFLAPNVSSVAFNTSWELPASLPLISIPYSMHCCVLGFLSCSLFLHMSFELKLLLLLLWLVASCSLFLHSHAWLSDCLIARLYQGSLGSRPGVLKEPKLMGAIYFFIFFFTLLVLARQNEYYCRLDFLWKKKLRQEREETETMENLTRLLLENVLPAHVAPQFIGQNRRNEDLYHQSYECVCVLFASIPDFKEFYSESNINHEGLECLRLLNEIIADFDELLSKPKFSGVEKIKTIGSTYMAATGLNATPGQDTQQDAERSCSHLGTMVEFAVALGSKLGVINKHSFNNFRLRVGLNHGPVVAGVIGAQKPQYDIWGNTVNVASRMESTGVLGKIQVTEETARALQSLGYTCYSRGVIKVKGKGQLCTYFLNTDLTRTGSPSAS

Target ORF information:

RefSeq Version	NM_019285.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adenylate cyclase 4 (Adcy4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019285.2

ORF Insert Sequence:

ATGGCCCGCC TCTTCAGTCC CCGGCCTCCT CCCAGCGAAG ATCTCTTCTA CGAGACTTAC 
TACAGCCTGA GCCAACAGTA TCCACTGCTA ATTCTGCTTC TGGTCATTGT GCTCTGCGCG 
ATCGTGGCAC TGCCCGCCGT CGCCTGGGCC AGCGGCAGGG AGCTGACCTC AGACCCAAGC 
TTCCTGACAA CTGTGCTGTG TGCTTTGGGT GGCTTCTCCC TGCTTTTGGG GCTCGCTTCC 
CGGGAGCAGC AACTGCAGCG ATGGACACGA CCTCTTTCTG GCCTCATATG GGCTGCATTG 
CTGGCTCTGG GCTATGGGTT TCTATTCACT GGGGGTGTGG TGAGCGCCTG GGATCAGGTG 
TCCTTTTTCC TCTTCATCAT TTTCACCGTG TACGCCATGC TGCCCTTGGG CATGCGGGAT 
GCTGCTGCCG CGGGGGTCAT CTCATCCCTC TCACACCTGC TGGTCCTTGG ACTGTACCTT 
GGGTGGCGGC CTGAGTCGCA GAGGGATCTG CTACCACAGT TGGCAGCGAA TGCGGTGTTG 
TTCCTGTGTG GGAACGTGGT GGGAGCATAC CACAAGGCCC TGATGGAGCG AGCATTGCGC 
GCCACGTTCC GGGAGGCTCT TAGCTCCCTG CATTCACGCC GGAGGTTGGA CACTGAGAAA 
AAGCACCAGG AGCACCTCCT CTTGTCTATC CTTCCTGCCT ACCTGGCCCG AGAGATGAAG 
GCAGAGATCA TGGCTCGGCT GCAGGCTGGA CAGAGCTCAC GGCCAGAGAA CACAAACAAC 
TTTCACAGCC TGTATGTCAA GAGGCACCAA GGAGTGAGTG TGCTGTATGC TGACATCGTG 
GGCTTCACAC GGCTGGCCAG TGAGTGTTCC CCTAAGGAGC TGGTGCTAAT GCTCAATGAA 
CTCTTCGGCA AATTCGACCA AATTGCAAAG GAGCACGAAT GCATGCGGAT CAAGATCCTG 
GGAGACTGTT ACTACTGTGT ATCCGGGCTG CCCCTCTCTC TGCCTGACCA CGCCATCAAT 
TGCGTGCGCA TGGGACTGGA CATGTGCCGG GCCATCAGGA AACTTCGGGT AGCCACCGGT 
GTGGATATCA ACATGCGTGT CGGTGTGCAC TCAGGCAGCG TCCTCTGTGG AGTCATCGGG 
CTACAGAAGT GGCAGTATGA TGTCTGGTCC CATGATGTCA CATTGGCCAA CCATATGGAA 
GCAGGCGGCG TTCCAGGACG AGTGCACATC ACAGGGGCCA CGCTGGCCCT GCTGGCAGGA 
GCTTATGCTG TGGAGAGAGC AGACATGGAG CATCGAGACC CATACCTTCG GGAGCTAGGG 
GAACCCACAT ACCTGGTCAT TGATCCTTGG GCTGAGGAGG AAGACGAGAA GGGCACCGAG 
AGAGGATTGC TGTCTTCTCT AGAGGGGCAC ACGATGCGTC CGTCACTACT GATGACTCGT 
TATCTGGAGT CTTGGGGTGC AGCCAAGCCT TTCGCCCACC TAAGCCACGT CGACAGTCCT 
GCATCCACAT CCACTCCACT CCCGGAGAAA GCCTTCAGCC CCCAGTGGAG CCTGGACCGG 
AGTCGCACCC CCCGCGGGCT ACACGATGAA CTGGACACTG GAGATGCCAA GTTCTTCCAG 
GTCATCGAAC AACTCAACTC TCAAAAACAG TGGAAACAGT CGAAGGACTT CAACCTCCTG 
ACGCTCTACT TCAGAGAGAA GGAGATGGAG AAACAGTATC GGCTGTCTGC GCTCCCCGCC 
TTCAAATACT ACGCAGCCTG CACCTTCCTG GTTTTTCTGT CCAACTTCAC AATCCAAATG 
CTGGTGACAA CCAGGCCCCC AGCTCTGGCC ACCACCTACA GCATCACCTT CCTTCTCTTC 
CTTCTCCTCC TCTTCGTCTG CTTCTCAGAG CACCTGACGA AGTGCGTCCA GAAAGGCCCC 
AAGATGTTGC ACTGGCTGCC TGCGCTGTCT GTCCTGGTGG CCACACGGCC GGGATTGCGA 
GTAGCCCTGG GCACAGCCAC CATCCTCCTG GTGTTCACTA TGGCCGTCGT CAGCCTGCTC 
TTCTTACCAG TGTCGTCAGA CTGCCCTTTC CTGGCTCCCA ATGTGTCGTC AGTGGCTTTT 
AATACTTCCT GGGAGCTGCC AGCATCCCTG CCTCTCATCA GCATCCCATA CTCCATGCAT 
TGTTGCGTGC TGGGTTTCCT TTCCTGCTCC CTTTTTCTGC ACATGAGCTT CGAACTGAAG 
CTGCTTCTGC TTCTGCTGTG GCTGGTGGCA TCTTGTTCCC TATTTCTGCA CTCCCACGCC 
TGGCTGTCCG ACTGCCTCAT TGCCCGCCTT TATCAAGGCT CATTGGGCTC CAGGCCGGGG 
GTACTGAAGG AACCGAAACT GATGGGAGCT ATCTACTTCT TCATCTTCTT CTTCACACTC 
CTCGTCCTGG CTCGGCAGAA TGAGTATTAC TGTCGCCTGG ACTTCCTATG GAAAAAGAAA 
CTGAGGCAGG AGCGAGAGGA AACTGAGACA ATGGAGAACC TGACCCGGCT CCTCCTGGAG 
AATGTACTCC CTGCACACGT GGCGCCCCAG TTCATCGGCC AGAACCGGCG CAATGAGGAC 
CTCTACCATC AGTCGTACGA ATGTGTTTGT GTCCTCTTTG CATCCATCCC AGACTTTAAG 
GAATTCTACT CGGAATCCAA CATCAACCAT GAGGGGCTAG AGTGTCTGCG GCTGCTCAAT 
GAGATCATTG CCGACTTTGA TGAGCTGCTC TCCAAGCCAA AGTTCAGTGG AGTAGAGAAG 
ATCAAAACTA TCGGCAGCAC CTACATGGCG GCCACAGGCC TAAATGCCAC CCCTGGGCAG 
GACACACAAC AGGACGCTGA GAGAAGCTGC AGCCATCTGG GCACCATGGT GGAATTTGCA 
GTGGCCCTGG GGTCTAAGCT GGGTGTCATC AATAAGCACT CGTTCAACAA CTTCCGCCTG 
CGTGTGGGGT TAAACCATGG ACCAGTCGTA GCAGGGGTGA TTGGGGCGCA GAAGCCACAA 
TATGACATCT GGGGGAATAC AGTGAACGTG GCCAGCCGCA TGGAGAGCAC AGGAGTTCTC 
GGCAAGATCC AAGTGACCGA GGAGACAGCT AGGGCTCTGC AATCCCTGGG TTACACATGC 
TACAGCCGAG GTGTCATCAA GGTCAAGGGC AAAGGGCAAC TCTGTACCTA CTTCCTGAAC 
ACAGACCTGA CACGAACCGG ATCTCCCTCA GCATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adcy4 ( XM_017599787.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455276.1 Rattus norvegicus adenylate cyclase 4 (Adcy4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04278
Clone ID Related Accession (Same CDS sequence)	XM_017599787.1 , NM_019285.2
Accession Version	XM_017599787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	adenylate cyclase type 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGCC TCTTCAGTCC CCGGCCTCCT CCCAGCGAAG ATCTCTTCTA CGAGACTTAC 
TACAGCCTGA GCCAACAGTA TCCACTGCTA ATTCTGCTTC TGGTCATTGT GCTCTGCGCG 
ATCGTGGCAC TGCCCGCCGT CGCCTGGGCC AGCGGCAGGG AGCTGACCTC AGACCCAAGC 
TTCCTGACAA CTGTGCTGTG TGCTTTGGGT GGCTTCTCCC TGCTTTTGGG GCTCGCTTCC 
CGGGAGCAGC AACTGCAGCG ATGGACACGA CCTCTTTCTG GCCTCATATG GGCTGCATTG 
CTGGCTCTGG GCTATGGGTT TCTATTCACT GGGGGTGTGG TGAGCGCCTG GGATCAGGTG 
TCCTTTTTCC TCTTCATCAT TTTCACCGTG TACGCCATGC TGCCCTTGGG CATGCGGGAT 
GCTGCTGCCG CGGGGGTCAT CTCATCCCTC TCACACCTGC TGGTCCTTGG ACTGTACCTT 
GGGTGGCGGC CTGAGTCGCA GAGGGATCTG CTACCACAGT TGGCAGCGAA TGCGGTGTTG 
TTCCTGTGTG GGAACGTGGT GGGAGCATAC CACAAGGCCC TGATGGAGCG AGCATTGCGC 
GCCACGTTCC GGGAGGCTCT TAGCTCCCTG CATTCACGCC GGAGGTTGGA CACTGAGAAA 
AAGCACCAGG AGCACCTCCT CTTGTCTATC CTTCCTGCCT ACCTGGCCCG AGAGATGAAG 
GCAGAGATCA TGGCTCGGCT GCAGGCTGGA CAGAGCTCAC GGCCAGAGAA CACAAACAAC 
TTTCACAGCC TGTATGTCAA GAGGCACCAA GGAGTGAGTG TGCTGTATGC TGACATCGTG 
GGCTTCACAC GGCTGGCCAG TGAGTGTTCC CCTAAGGAGC TGGTGCTAAT GCTCAATGAA 
CTCTTCGGCA AATTCGACCA AATTGCAAAG GAGCACGAAT GCATGCGGAT CAAGATCCTG 
GGAGACTGTT ACTACTGTGT ATCCGGGCTG CCCCTCTCTC TGCCTGACCA CGCCATCAAT 
TGCGTGCGCA TGGGACTGGA CATGTGCCGG GCCATCAGGA AACTTCGGGT AGCCACCGGT 
GTGGATATCA ACATGCGTGT CGGTGTGCAC TCAGGCAGCG TCCTCTGTGG AGTCATCGGG 
CTACAGAAGT GGCAGTATGA TGTCTGGTCC CATGATGTCA CATTGGCCAA CCATATGGAA 
GCAGGCGGCG TTCCAGGACG AGTGCACATC ACAGGGGCCA CGCTGGCCCT GCTGGCAGGA 
GCTTATGCTG TGGAGAGAGC AGACATGGAG CATCGAGACC CATACCTTCG GGAGCTAGGG 
GAACCCACAT ACCTGGTCAT TGATCCTTGG GCTGAGGAGG AAGACGAGAA GGGCACCGAG 
AGAGGATTGC TGTCTTCTCT AGAGGGGCAC ACGATGCGTC CGTCACTACT GATGACTCGT 
TATCTGGAGT CTTGGGGTGC AGCCAAGCCT TTCGCCCACC TAAGCCACGT CGACAGTCCT 
GCATCCACAT CCACTCCACT CCCGGAGAAA GCCTTCAGCC CCCAGTGGAG CCTGGACCGG 
AGTCGCACCC CCCGCGGGCT ACACGATGAA CTGGACACTG GAGATGCCAA GTTCTTCCAG 
GTCATCGAAC AACTCAACTC TCAAAAACAG TGGAAACAGT CGAAGGACTT CAACCTCCTG 
ACGCTCTACT TCAGAGAGAA GGAGATGGAG AAACAGTATC GGCTGTCTGC GCTCCCCGCC 
TTCAAATACT ACGCAGCCTG CACCTTCCTG GTTTTTCTGT CCAACTTCAC AATCCAAATG 
CTGGTGACAA CCAGGCCCCC AGCTCTGGCC ACCACCTACA GCATCACCTT CCTTCTCTTC 
CTTCTCCTCC TCTTCGTCTG CTTCTCAGAG CACCTGACGA AGTGCGTCCA GAAAGGCCCC 
AAGATGTTGC ACTGGCTGCC TGCGCTGTCT GTCCTGGTGG CCACACGGCC GGGATTGCGA 
GTAGCCCTGG GCACAGCCAC CATCCTCCTG GTGTTCACTA TGGCCGTCGT CAGCCTGCTC 
TTCTTACCAG TGTCGTCAGA CTGCCCTTTC CTGGCTCCCA ATGTGTCGTC AGTGGCTTTT 
AATACTTCCT GGGAGCTGCC AGCATCCCTG CCTCTCATCA GCATCCCATA CTCCATGCAT 
TGTTGCGTGC TGGGTTTCCT TTCCTGCTCC CTTTTTCTGC ACATGAGCTT CGAACTGAAG 
CTGCTTCTGC TTCTGCTGTG GCTGGTGGCA TCTTGTTCCC TATTTCTGCA CTCCCACGCC 
TGGCTGTCCG ACTGCCTCAT TGCCCGCCTT TATCAAGGCT CATTGGGCTC CAGGCCGGGG 
GTACTGAAGG AACCGAAACT GATGGGAGCT ATCTACTTCT TCATCTTCTT CTTCACACTC 
CTCGTCCTGG CTCGGCAGAA TGAGTATTAC TGTCGCCTGG ACTTCCTATG GAAAAAGAAA 
CTGAGGCAGG AGCGAGAGGA AACTGAGACA ATGGAGAACC TGACCCGGCT CCTCCTGGAG 
AATGTACTCC CTGCACACGT GGCGCCCCAG TTCATCGGCC AGAACCGGCG CAATGAGGAC 
CTCTACCATC AGTCGTACGA ATGTGTTTGT GTCCTCTTTG CATCCATCCC AGACTTTAAG 
GAATTCTACT CGGAATCCAA CATCAACCAT GAGGGGCTAG AGTGTCTGCG GCTGCTCAAT 
GAGATCATTG CCGACTTTGA TGAGCTGCTC TCCAAGCCAA AGTTCAGTGG AGTAGAGAAG 
ATCAAAACTA TCGGCAGCAC CTACATGGCG GCCACAGGCC TAAATGCCAC CCCTGGGCAG 
GACACACAAC AGGACGCTGA GAGAAGCTGC AGCCATCTGG GCACCATGGT GGAATTTGCA 
GTGGCCCTGG GGTCTAAGCT GGGTGTCATC AATAAGCACT CGTTCAACAA CTTCCGCCTG 
CGTGTGGGGT TAAACCATGG ACCAGTCGTA GCAGGGGTGA TTGGGGCGCA GAAGCCACAA 
TATGACATCT GGGGGAATAC AGTGAACGTG GCCAGCCGCA TGGAGAGCAC AGGAGTTCTC 
GGCAAGATCC AAGTGACCGA GGAGACAGCT AGGGCTCTGC AATCCCTGGG TTACACATGC 
TACAGCCGAG GTGTCATCAA GGTCAAGGGC AAAGGGCAAC TCTGTACCTA CTTCCTGAAC 
ACAGACCTGA CACGAACCGG ATCTCCCTCA GCATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455276.1
CDS	34..3252
Translation	MARLFSPRPPPSEDLFYETYYSLSQQYPLLILLLVIVLCAIVALPAVAWASGRELTSDPSFLTTVLCALGGFSLLLGLASREQQLQRWTRPLSGLIWAALLALGYGFLFTGGVVSAWDQVSFFLFIIFTVYAMLPLGMRDAAAAGVISSLSHLLVLGLYLGWRPESQRDLLPQLAANAVLFLCGNVVGAYHKALMERALRATFREALSSLHSRRRLDTEKKHQEHLLLSILPAYLAREMKAEIMARLQAGQSSRPENTNNFHSLYVKRHQGVSVLYADIVGFTRLASECSPKELVLMLNELFGKFDQIAKEHECMRIKILGDCYYCVSGLPLSLPDHAINCVRMGLDMCRAIRKLRVATGVDINMRVGVHSGSVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHITGATLALLAGAYAVERADMEHRDPYLRELGEPTYLVIDPWAEEEDEKGTERGLLSSLEGHTMRPSLLMTRYLESWGAAKPFAHLSHVDSPASTSTPLPEKAFSPQWSLDRSRTPRGLHDELDTGDAKFFQVIEQLNSQKQWKQSKDFNLLTLYFREKEMEKQYRLSALPAFKYYAACTFLVFLSNFTIQMLVTTRPPALATTYSITFLLFLLLLFVCFSEHLTKCVQKGPKMLHWLPALSVLVATRPGLRVALGTATILLVFTMAVVSLLFLPVSSDCPFLAPNVSSVAFNTSWELPASLPLISIPYSMHCCVLGFLSCSLFLHMSFELKLLLLLLWLVASCSLFLHSHAWLSDCLIARLYQGSLGSRPGVLKEPKLMGAIYFFIFFFTLLVLARQNEYYCRLDFLWKKKLRQEREETETMENLTRLLLENVLPAHVAPQFIGQNRRNEDLYHQSYECVCVLFASIPDFKEFYSESNINHEGLECLRLLNEIIADFDELLSKPKFSGVEKIKTIGSTYMAATGLNATPGQDTQQDAERSCSHLGTMVEFAVALGSKLGVINKHSFNNFRLRVGLNHGPVVAGVIGAQKPQYDIWGNTVNVASRMESTGVLGKIQVTEETARALQSLGYTCYSRGVIKVKGKGQLCTYFLNTDLTRTGSPSAS

Target ORF information:

RefSeq Version	XM_017599787.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adenylate cyclase 4 (Adcy4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599787.1

ORF Insert Sequence:

ATGGCCCGCC TCTTCAGTCC CCGGCCTCCT CCCAGCGAAG ATCTCTTCTA CGAGACTTAC 
TACAGCCTGA GCCAACAGTA TCCACTGCTA ATTCTGCTTC TGGTCATTGT GCTCTGCGCG 
ATCGTGGCAC TGCCCGCCGT CGCCTGGGCC AGCGGCAGGG AGCTGACCTC AGACCCAAGC 
TTCCTGACAA CTGTGCTGTG TGCTTTGGGT GGCTTCTCCC TGCTTTTGGG GCTCGCTTCC 
CGGGAGCAGC AACTGCAGCG ATGGACACGA CCTCTTTCTG GCCTCATATG GGCTGCATTG 
CTGGCTCTGG GCTATGGGTT TCTATTCACT GGGGGTGTGG TGAGCGCCTG GGATCAGGTG 
TCCTTTTTCC TCTTCATCAT TTTCACCGTG TACGCCATGC TGCCCTTGGG CATGCGGGAT 
GCTGCTGCCG CGGGGGTCAT CTCATCCCTC TCACACCTGC TGGTCCTTGG ACTGTACCTT 
GGGTGGCGGC CTGAGTCGCA GAGGGATCTG CTACCACAGT TGGCAGCGAA TGCGGTGTTG 
TTCCTGTGTG GGAACGTGGT GGGAGCATAC CACAAGGCCC TGATGGAGCG AGCATTGCGC 
GCCACGTTCC GGGAGGCTCT TAGCTCCCTG CATTCACGCC GGAGGTTGGA CACTGAGAAA 
AAGCACCAGG AGCACCTCCT CTTGTCTATC CTTCCTGCCT ACCTGGCCCG AGAGATGAAG 
GCAGAGATCA TGGCTCGGCT GCAGGCTGGA CAGAGCTCAC GGCCAGAGAA CACAAACAAC 
TTTCACAGCC TGTATGTCAA GAGGCACCAA GGAGTGAGTG TGCTGTATGC TGACATCGTG 
GGCTTCACAC GGCTGGCCAG TGAGTGTTCC CCTAAGGAGC TGGTGCTAAT GCTCAATGAA 
CTCTTCGGCA AATTCGACCA AATTGCAAAG GAGCACGAAT GCATGCGGAT CAAGATCCTG 
GGAGACTGTT ACTACTGTGT ATCCGGGCTG CCCCTCTCTC TGCCTGACCA CGCCATCAAT 
TGCGTGCGCA TGGGACTGGA CATGTGCCGG GCCATCAGGA AACTTCGGGT AGCCACCGGT 
GTGGATATCA ACATGCGTGT CGGTGTGCAC TCAGGCAGCG TCCTCTGTGG AGTCATCGGG 
CTACAGAAGT GGCAGTATGA TGTCTGGTCC CATGATGTCA CATTGGCCAA CCATATGGAA 
GCAGGCGGCG TTCCAGGACG AGTGCACATC ACAGGGGCCA CGCTGGCCCT GCTGGCAGGA 
GCTTATGCTG TGGAGAGAGC AGACATGGAG CATCGAGACC CATACCTTCG GGAGCTAGGG 
GAACCCACAT ACCTGGTCAT TGATCCTTGG GCTGAGGAGG AAGACGAGAA GGGCACCGAG 
AGAGGATTGC TGTCTTCTCT AGAGGGGCAC ACGATGCGTC CGTCACTACT GATGACTCGT 
TATCTGGAGT CTTGGGGTGC AGCCAAGCCT TTCGCCCACC TAAGCCACGT CGACAGTCCT 
GCATCCACAT CCACTCCACT CCCGGAGAAA GCCTTCAGCC CCCAGTGGAG CCTGGACCGG 
AGTCGCACCC CCCGCGGGCT ACACGATGAA CTGGACACTG GAGATGCCAA GTTCTTCCAG 
GTCATCGAAC AACTCAACTC TCAAAAACAG TGGAAACAGT CGAAGGACTT CAACCTCCTG 
ACGCTCTACT TCAGAGAGAA GGAGATGGAG AAACAGTATC GGCTGTCTGC GCTCCCCGCC 
TTCAAATACT ACGCAGCCTG CACCTTCCTG GTTTTTCTGT CCAACTTCAC AATCCAAATG 
CTGGTGACAA CCAGGCCCCC AGCTCTGGCC ACCACCTACA GCATCACCTT CCTTCTCTTC 
CTTCTCCTCC TCTTCGTCTG CTTCTCAGAG CACCTGACGA AGTGCGTCCA GAAAGGCCCC 
AAGATGTTGC ACTGGCTGCC TGCGCTGTCT GTCCTGGTGG CCACACGGCC GGGATTGCGA 
GTAGCCCTGG GCACAGCCAC CATCCTCCTG GTGTTCACTA TGGCCGTCGT CAGCCTGCTC 
TTCTTACCAG TGTCGTCAGA CTGCCCTTTC CTGGCTCCCA ATGTGTCGTC AGTGGCTTTT 
AATACTTCCT GGGAGCTGCC AGCATCCCTG CCTCTCATCA GCATCCCATA CTCCATGCAT 
TGTTGCGTGC TGGGTTTCCT TTCCTGCTCC CTTTTTCTGC ACATGAGCTT CGAACTGAAG 
CTGCTTCTGC TTCTGCTGTG GCTGGTGGCA TCTTGTTCCC TATTTCTGCA CTCCCACGCC 
TGGCTGTCCG ACTGCCTCAT TGCCCGCCTT TATCAAGGCT CATTGGGCTC CAGGCCGGGG 
GTACTGAAGG AACCGAAACT GATGGGAGCT ATCTACTTCT TCATCTTCTT CTTCACACTC 
CTCGTCCTGG CTCGGCAGAA TGAGTATTAC TGTCGCCTGG ACTTCCTATG GAAAAAGAAA 
CTGAGGCAGG AGCGAGAGGA AACTGAGACA ATGGAGAACC TGACCCGGCT CCTCCTGGAG 
AATGTACTCC CTGCACACGT GGCGCCCCAG TTCATCGGCC AGAACCGGCG CAATGAGGAC 
CTCTACCATC AGTCGTACGA ATGTGTTTGT GTCCTCTTTG CATCCATCCC AGACTTTAAG 
GAATTCTACT CGGAATCCAA CATCAACCAT GAGGGGCTAG AGTGTCTGCG GCTGCTCAAT 
GAGATCATTG CCGACTTTGA TGAGCTGCTC TCCAAGCCAA AGTTCAGTGG AGTAGAGAAG 
ATCAAAACTA TCGGCAGCAC CTACATGGCG GCCACAGGCC TAAATGCCAC CCCTGGGCAG 
GACACACAAC AGGACGCTGA GAGAAGCTGC AGCCATCTGG GCACCATGGT GGAATTTGCA 
GTGGCCCTGG GGTCTAAGCT GGGTGTCATC AATAAGCACT CGTTCAACAA CTTCCGCCTG 
CGTGTGGGGT TAAACCATGG ACCAGTCGTA GCAGGGGTGA TTGGGGCGCA GAAGCCACAA 
TATGACATCT GGGGGAATAC AGTGAACGTG GCCAGCCGCA TGGAGAGCAC AGGAGTTCTC 
GGCAAGATCC AAGTGACCGA GGAGACAGCT AGGGCTCTGC AATCCCTGGG TTACACATGC 
TACAGCCGAG GTGTCATCAA GGTCAAGGGC AAAGGGCAAC TCTGTACCTA CTTCCTGAAC 
ACAGACCTGA CACGAACCGG ATCTCCCTCA GCATCCTAG