Cps1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cps1
Entrez Gene ID	497840
Full Name	carbamoyl-phosphate synthase 1
Synonyms	cps,cps-1
General protein information	Preferred Names carbamoyl-phosphate synthase 1 Names carbamoyl-phosphate synthase [ammonia], mitochondrial CPSase I carbamoyl-phosphate synthetase 1, mitochondrial carbamoyl-phosphate synthetase I carbamyl phosphate synthetase I carbamyl-phosphate synthetase carboamyl-phosphate synthetase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q32
Summary	catalyzes synthesis of carbamyl phosphate from NH3, HCO3, and 2ATP [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017072.2	NP_058768.1	carbamoyl-phosphate synthase [ammonia], mitochondrial precursor
XM_017596592.1	XP_017452081.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CPS1	NP_001179187.1
Xenopus tropicalis (tropical clawed frog)	cps1	XP_004917905.1
Homo sapiens (human)	CPS1	NP_001116105.1
Rattus norvegicus (Norway rat)	Cps1	NP_058768.1
Pan troglodytes (chimpanzee)	CPS1	XP_001146604.2
Canis lupus familiaris (dog)	CPS1	XP_545627.2
Mus musculus (house mouse)	Cps1	NP_001074278.1
Danio rerio (zebrafish)	cps1	XP_683282.4
Oryza sativa (rice)	Os01g0570700	NP_001043373.1
Macaca mulatta (Rhesus monkey)	CPS1	XP_001110375.1
Gallus gallus (chicken)	CPS1	NP_001039306.1
Arabidopsis thaliana (thale cress)	CARB	NP_564338.1

Gene Ontology
Bibliography

Related articles in PubMed

Conditional disruption of hepatic carbamoyl phosphate synthetase 1 in mice results in hyperammonemia without orotic aciduria and can be corrected by liver-directed gene therapy.
Khoja S, Nitzahn M, Hermann K, Truong B, Borzone R, Willis B, Rudd M, Palmer DJ, Ng P, Brunetti-Pierri N, Lipshutz GS
Molecular genetics and metabolism124(4)243-253(2018 08)

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
Martin J, Balmer ML, Rajendran S, Maurhofer O, Dufour JF, St-Pierre MV
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 04)

Isoaspartate, carbamoyl phosphate synthase-1, and carbonic anhydrase-III as biomarkers of liver injury.
Carter WG, Vigneswara V, Newlaczyl A, Wayne D, Ahmed B, Saddington S, Brewer C, Raut N, Gerdes HK, Erdozain AM, Tooth D, Bolt EL, Osna NA, Tuma DJ, Kharbanda KK
Biochemical and biophysical research communications458(3)626-631(2015 Mar)

Protein tyrosine nitration of mitochondrial carbamoyl phosphate synthetase 1 and its functional consequences.
Takakusa H, Mohar I, Kavanagh TJ, Kelly EJ, Kaspera R, Nelson SD
Biochemical and biophysical research communications420(1)54-60(2012 Mar)

Molecular defects in human carbamoy phosphate synthetase I: mutational spectrum, diagnostic and protein structure considerations.
Häberle J, Shchelochkov OA, Wang J, Katsonis P, Hall L, Reiss S, Eeds A, Willis A, Yadav M, Summar S, , Lichtarge O, Rubio V, Wong LJ, Summar M
Human mutation32(6)579-89(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cps1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cps1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13342	NM_017072.2 Latest version!	Rattus norvegicus carbamoyl-phosphate synthase 1 (Cps1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa13342	XM_017596592.1 Latest version!	Rattus norvegicus carbamoyl-phosphate synthase 1 (Cps1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cps1 ( NM_017072.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058768.1 Rattus norvegicus carbamoyl-phosphate synthase 1 (Cps1), mRNA.

Price & Availability↑

CloneID	ORa13342
Clone ID Related Accession (Same CDS sequence)	NM_017072.2 , XM_017596592.1
Accession Version	NM_017072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07067956.1. On Apr 15, 2016 this sequence version replaced NM_017072.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906348, SAMN01906354 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGACGAGGA TTTTGACAGC ATGCAAAGTG GTGAAGACAC TGAAGAGTGG CTTTGGTTTG 
GCCAATGTGA CTTCGAAGCG ACAGTGGGAC TTCTCTAGAC CTGGCATCAG GCTCCTTTCT 
GTGAAGGCAC AGACAGCACA CATTGTTCTG GAAGATGGAA CTAAGATGAA GGGCTACTCC 
TTTGGCCATC CCTCCTCGGT TGCTGGCGAA GTGGTTTTTA ATACTGGCTT AGGAGGGTAC 
TCGGAAGCAC TTACTGATCC TGCCTACAAG GGGCAGATCC TCACCATGGC CAACCCTATC 
ATTGGGAATG GTGGGGCCCC TGATACAACG GCACGTGATG AACTGGGACT GAATAAGTAC 
ATGGAGTCTG ATGGAATCAA GGTGGCGGGT CTGCTGGTGC TGAATTACAG TCATGACTAC 
AACCACTGGC TGGCCACCAA GAGTCTGGGT CAGTGGCTGC AGGAGGAGAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT 
GGGAAGATTG AGTTTGAGGG CCAGTCTGTG GACTTTGTGG ATCCTAATAA GCAGAATTTG 
ATTGCCGAGG TTTCAACCAA GGATGTCAAG GTGTTTGGCA AAGGAAACCC CACGAAAGTG 
GTAGCCGTGG ACTGTGGGAT AAAAAACAAT GTCATCCGCC TGCTAGTTAA GCGAGGAGCG 
GAAGTGCATT TGGTCCCCTG GAATCATGAC TTCACCCAGA TGGACTATGA CGGACTTCTG 
ATCGCTGGAG GACCTGGGAA CCCAGCTCTG GCACAGCCAC TAATTCAGAA CGTGAAGAAG 
ATTTTGGAGA GTGACCGCAA AGAGCCGTTG TTTGGAATCA GTACAGGAAA CATAATAACA 
GGATTGGCTG CTGGCGCCAA ATCCTACAAG ATGTCCATGG CCAACAGAGG ACAGAACCAA 
CCTGTTTTGA ATATCACAAA CAGACAGGCT TTCATAACTG CTCAGAATCA TGGCTATGCT 
CTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTGTTTG TCAATGTCAA TGACCAAACA 
AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC 
AGCCCGGGGC CAACAGACAC TGAGTACCTA TTTGATTCCT TCTTCTCGCT GATAAAGAAG 
GGCAAAGGCA CCACCATTAC CTCAGTTCTG CCCAAGCCAG CATTGGTGGC ATCTCGAGTC 
GAGGTTTCCA AGGTCCTTAT CCTAGGATCA GGAGGCCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CCGGATCTCA GGCTGTAAAA GCCATGAAGG AAGAAAACGT CAAAACAGTC 
CTGATGAACC CGAACATTGC ATCCGTGCAG ACCAACGAGG TGGGATTGAA GCAGGCAGAT 
GCAGTCTACT TTCTCCCTAT CACCCCCCAG TTTGTCACAG AAGTTATCAA GGCTGAACGG 
CCCGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA 
TTCAAGAGGG GTGTGCTCAA GGAATATGGT GTGAAGGTCT TGGGAACATC GGTCGAGTCC 
ATTATGGCCA CAGAAGACAG GCAGCTTTTC TCAGACAAGC TGAATGAGAT CAACGAGAAG 
ATTGCTCCTA GCTTTGCAGT GGAATCAATG GAGGATGCCT TGAAGGCAGC AGACACCATT 
GGCTACCCTG TGATGATTCG GTCTGCGTAT GCTCTGGGTG GGTTAGGCTC CGGCATCTGT 
CCCAACAAGG AGACCTTAAT GGATCTTGGC ACAAAGGCAT TTGCTATGAC CAACCAGATC 
CTGGTGGAGC GGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTTGT CCGAGATGCG 
GATGATAACT GTGTCACAGT CTGTAACATG GAGAATGTTG ACGCCATGGG TGTTCACACA 
GGTGATTCAG TTGTTGTGGC CCCGGCCCAG ACCCTCTCCA ATGCAGAGTT CCAGATGTTG 
AGACGCACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG 
TTTGCTCTTC ACCCCACTTC CATGGAATAC TGTATCATTG AAGTGAATGC CAGGCTCTCC 
AGGAGTTCTG CCCTGGCCTC CAAGGCCACT GGCTACCCAC TGGCGTTCAT CGCAGCAAAG 
ATCGCTCTAG GAATCCCACT TCCAGAAATC AAGAATGTTG TGTCTGGGAA GACCTCAGCC 
TGCTTCGAAC CTAGCCTGGA TTACATGGTG ACCAAGATTC CTCGCTGGGA CCTTGACCGT 
TTTCATGGAA CATCCAGCCG AATTGGTAGC TCAATGAAAA GTGTAGGCGA GGTCATGGCC 
ATTGGTCGCA CCTTTGAGGA AAGTTTCCAG AAGGCTCTGA GGATGTGCCA TCCATCTGTG 
GATGGGTTCA CTCCCCGTCT CCCAATGAAT AAGGAATGGC CAGCAAACCT GGATCTGAGG 
AAGGAGCTGT CTGAACCCTC CAGCACCCGC ATCTATGCCA TTGCTAAGGC CTTGGAAAAC 
AACATGTCCC TTGACGAAAT CGTGAAGCTC ACATCCATTG ACAAGTGGTT TTTGTATAAG 
ATGCGTGACA TTTTAAACAT GGATAAGACA CTGAAAGGGC TTAACAGTGA GTCTGTTACA 
GAAGAAACTC TGAGACAGGC CAAAGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGT 
TTAGGACTGA CCGAGGCTCA GACAAGAGAG CTGAGATTGA AGAAAAACAT CCACCCTTGG 
GTTAAACAGA TTGATACATT GGCTGCAGAA TACCCATCAG TGACAAATTA CCTGTATGTT 
ACCTACAATG GCCAGGAGCA CGACATCAAA TTTGATGAAC ATGGAATAAT GGTGCTGGGC 
TGTGGCCCAT ACCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACACTGC GCCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGTAACCC GGAGACTGTG 
AGCACTGACT TTGATGAGTG TGACAAACTC TACTTTGAAG AGCTGTCTTT GGAGAGAATC 
CTAGATATCT ACCACCAGGA GGCATGTAAT GGCTGCATCA TATCAGTCGG GGGCCAGATT 
CCAAACAACT TGGCGGTTCC GCTATACAAG AACGGTGTCA AGATCATGGG TACCAGTCCT 
CTGCAGATCG ATAGGGCTGA GGATCGCTCC ATCTTCTCGG CTGTCTTAGA TGAGCTGAAG 
GTGGCCCAGG CTCCTTGGAA AGCTGTTAAC ACTTTGAACG AGGCGCTGGA ATTTGCAAAC 
TCTGTGGGCT ATCCCTGCTT ACTGAGACCT TCCTATGTTT TGAGTGGGTC TGCCATGAAC 
GTGGTATTCT CTGAGGATGA GATGAAAAGG TTCCTTGAGG AGGCCACTCG AGTCTCTCAG 
GAACACCCAG TGGTGCTGAC CAAGTTTATT GAGGGGGCTC GGGAAGTGGA GATGGACGCT 
GTTGGCAAAG AAGGACGGGT CATCTCCCAT GCCATCTCTG AACATGTTGA AGATGCAGGT 
GTCCACTCAG GGGATGCCAC ACTGATGCTA CCTACGCAGA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCCAC ACGAAAGATT GCAAAGGCTT TTGCCATCTC TGGGCCATTC 
AATGTTCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGCAA TCTGAGAGCC 
TCTCGATCCT TCCCCTTTGT CTCCAAGACT CTTGGGGTGG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TCGGAGAGAG TGTTGATGAG AAGCATCTAC CCACACTGGA ACAACCCATC 
ATCCCCTCTG ACTATGTTGC CATTAAGGCT CCCATGTTTT CCTGGCCCCG ACTGAGGGAT 
GCTGATCCTA TTCTGAGATG TGAGATGGCT TCTACTGGAG AGGTGGCGTG TTTTGGTGAG 
GGCATTCATA CAGCCTTCCT AAAGGCAATG CTGTCCACAG GGTTTAAGAT ACCTCAGAAG 
GGCATTCTGA TTGGCATCCA GCAATCATTC CGTCCAAGAT TCCTTGGTGT TGCTGAGCAG 
TTACACAATG AAGGTTTCAA GCTTTTTGCC ACAGAAGCCA CATCAGACTG GCTCAACGCC 
AACAATGTTC CTGCCACCCC AGTGGCTTGG CCATCTCAGG AAGGACAGAA TCCCAGCCTC 
TCTTCCATCA GAAAGTTGAT AAGAGACGGA AGCATTGACC TAGTGATTAA CCTCCCCAAT 
AACAACACCA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT GGACAGTGGA 
ATTGCTCTGC TCACCAATTT CCAGGTGACC AAACTTTTTG CTGAGGCTGT GCAGAAAGCT 
CGTACTGTGG ACTCCAAGAG TCTGTTCCAC TACAGGCAGT ACAGCGCTGG AAAGGCAGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058768.1
CDS	336..4838
Translation	MTRILTACKVVKTLKSGFGLANVTSKRQWDFSRPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYSEALTDPAYKGQILTMANPIIGNGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSHDYNHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVKVFGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMDYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFDSFFSLIKKGKGTTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLMDLGTKAFAMTNQILVERSVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLRKELSEPSSTRIYAIAKALENNMSLDEIVKLTSIDKWFLYKMRDILNMDKTLKGLNSESVTEETLRQAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFDEHGIMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACNGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFANSVGYPCLLRPSYVLSGSAMNVVFSEDEMKRFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKEGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGVDFIDVATKVMIGESVDEKHLPTLEQPIIPSDYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVQKARTVDSKSLFHYRQYSAGKAA

Target ORF information:

RefSeq Version	NM_017072.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carbamoyl-phosphate synthase 1 (Cps1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017072.2

ORF Insert Sequence:

ATGACGAGGA TTTTGACAGC ATGCAAAGTG GTGAAGACAC TGAAGAGTGG CTTTGGTTTG 
GCCAATGTGA CTTCGAAGCG ACAGTGGGAC TTCTCTAGAC CTGGCATCAG GCTCCTTTCT 
GTGAAGGCAC AGACAGCACA CATTGTTCTG GAAGATGGAA CTAAGATGAA GGGCTACTCC 
TTTGGCCATC CCTCCTCGGT TGCTGGCGAA GTGGTTTTTA ATACTGGCTT AGGAGGGTAC 
TCGGAAGCAC TTACTGATCC TGCCTACAAG GGGCAGATCC TCACCATGGC CAACCCTATC 
ATTGGGAATG GTGGGGCCCC TGATACAACG GCACGTGATG AACTGGGACT GAATAAGTAC 
ATGGAGTCTG ATGGAATCAA GGTGGCGGGT CTGCTGGTGC TGAATTACAG TCATGACTAC 
AACCACTGGC TGGCCACCAA GAGTCTGGGT CAGTGGCTGC AGGAGGAGAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT 
GGGAAGATTG AGTTTGAGGG CCAGTCTGTG GACTTTGTGG ATCCTAATAA GCAGAATTTG 
ATTGCCGAGG TTTCAACCAA GGATGTCAAG GTGTTTGGCA AAGGAAACCC CACGAAAGTG 
GTAGCCGTGG ACTGTGGGAT AAAAAACAAT GTCATCCGCC TGCTAGTTAA GCGAGGAGCG 
GAAGTGCATT TGGTCCCCTG GAATCATGAC TTCACCCAGA TGGACTATGA CGGACTTCTG 
ATCGCTGGAG GACCTGGGAA CCCAGCTCTG GCACAGCCAC TAATTCAGAA CGTGAAGAAG 
ATTTTGGAGA GTGACCGCAA AGAGCCGTTG TTTGGAATCA GTACAGGAAA CATAATAACA 
GGATTGGCTG CTGGCGCCAA ATCCTACAAG ATGTCCATGG CCAACAGAGG ACAGAACCAA 
CCTGTTTTGA ATATCACAAA CAGACAGGCT TTCATAACTG CTCAGAATCA TGGCTATGCT 
CTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTGTTTG TCAATGTCAA TGACCAAACA 
AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC 
AGCCCGGGGC CAACAGACAC TGAGTACCTA TTTGATTCCT TCTTCTCGCT GATAAAGAAG 
GGCAAAGGCA CCACCATTAC CTCAGTTCTG CCCAAGCCAG CATTGGTGGC ATCTCGAGTC 
GAGGTTTCCA AGGTCCTTAT CCTAGGATCA GGAGGCCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CCGGATCTCA GGCTGTAAAA GCCATGAAGG AAGAAAACGT CAAAACAGTC 
CTGATGAACC CGAACATTGC ATCCGTGCAG ACCAACGAGG TGGGATTGAA GCAGGCAGAT 
GCAGTCTACT TTCTCCCTAT CACCCCCCAG TTTGTCACAG AAGTTATCAA GGCTGAACGG 
CCCGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA 
TTCAAGAGGG GTGTGCTCAA GGAATATGGT GTGAAGGTCT TGGGAACATC GGTCGAGTCC 
ATTATGGCCA CAGAAGACAG GCAGCTTTTC TCAGACAAGC TGAATGAGAT CAACGAGAAG 
ATTGCTCCTA GCTTTGCAGT GGAATCAATG GAGGATGCCT TGAAGGCAGC AGACACCATT 
GGCTACCCTG TGATGATTCG GTCTGCGTAT GCTCTGGGTG GGTTAGGCTC CGGCATCTGT 
CCCAACAAGG AGACCTTAAT GGATCTTGGC ACAAAGGCAT TTGCTATGAC CAACCAGATC 
CTGGTGGAGC GGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTTGT CCGAGATGCG 
GATGATAACT GTGTCACAGT CTGTAACATG GAGAATGTTG ACGCCATGGG TGTTCACACA 
GGTGATTCAG TTGTTGTGGC CCCGGCCCAG ACCCTCTCCA ATGCAGAGTT CCAGATGTTG 
AGACGCACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG 
TTTGCTCTTC ACCCCACTTC CATGGAATAC TGTATCATTG AAGTGAATGC CAGGCTCTCC 
AGGAGTTCTG CCCTGGCCTC CAAGGCCACT GGCTACCCAC TGGCGTTCAT CGCAGCAAAG 
ATCGCTCTAG GAATCCCACT TCCAGAAATC AAGAATGTTG TGTCTGGGAA GACCTCAGCC 
TGCTTCGAAC CTAGCCTGGA TTACATGGTG ACCAAGATTC CTCGCTGGGA CCTTGACCGT 
TTTCATGGAA CATCCAGCCG AATTGGTAGC TCAATGAAAA GTGTAGGCGA GGTCATGGCC 
ATTGGTCGCA CCTTTGAGGA AAGTTTCCAG AAGGCTCTGA GGATGTGCCA TCCATCTGTG 
GATGGGTTCA CTCCCCGTCT CCCAATGAAT AAGGAATGGC CAGCAAACCT GGATCTGAGG 
AAGGAGCTGT CTGAACCCTC CAGCACCCGC ATCTATGCCA TTGCTAAGGC CTTGGAAAAC 
AACATGTCCC TTGACGAAAT CGTGAAGCTC ACATCCATTG ACAAGTGGTT TTTGTATAAG 
ATGCGTGACA TTTTAAACAT GGATAAGACA CTGAAAGGGC TTAACAGTGA GTCTGTTACA 
GAAGAAACTC TGAGACAGGC CAAAGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGT 
TTAGGACTGA CCGAGGCTCA GACAAGAGAG CTGAGATTGA AGAAAAACAT CCACCCTTGG 
GTTAAACAGA TTGATACATT GGCTGCAGAA TACCCATCAG TGACAAATTA CCTGTATGTT 
ACCTACAATG GCCAGGAGCA CGACATCAAA TTTGATGAAC ATGGAATAAT GGTGCTGGGC 
TGTGGCCCAT ACCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACACTGC GCCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGTAACCC GGAGACTGTG 
AGCACTGACT TTGATGAGTG TGACAAACTC TACTTTGAAG AGCTGTCTTT GGAGAGAATC 
CTAGATATCT ACCACCAGGA GGCATGTAAT GGCTGCATCA TATCAGTCGG GGGCCAGATT 
CCAAACAACT TGGCGGTTCC GCTATACAAG AACGGTGTCA AGATCATGGG TACCAGTCCT 
CTGCAGATCG ATAGGGCTGA GGATCGCTCC ATCTTCTCGG CTGTCTTAGA TGAGCTGAAG 
GTGGCCCAGG CTCCTTGGAA AGCTGTTAAC ACTTTGAACG AGGCGCTGGA ATTTGCAAAC 
TCTGTGGGCT ATCCCTGCTT ACTGAGACCT TCCTATGTTT TGAGTGGGTC TGCCATGAAC 
GTGGTATTCT CTGAGGATGA GATGAAAAGG TTCCTTGAGG AGGCCACTCG AGTCTCTCAG 
GAACACCCAG TGGTGCTGAC CAAGTTTATT GAGGGGGCTC GGGAAGTGGA GATGGACGCT 
GTTGGCAAAG AAGGACGGGT CATCTCCCAT GCCATCTCTG AACATGTTGA AGATGCAGGT 
GTCCACTCAG GGGATGCCAC ACTGATGCTA CCTACGCAGA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCCAC ACGAAAGATT GCAAAGGCTT TTGCCATCTC TGGGCCATTC 
AATGTTCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGCAA TCTGAGAGCC 
TCTCGATCCT TCCCCTTTGT CTCCAAGACT CTTGGGGTGG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TCGGAGAGAG TGTTGATGAG AAGCATCTAC CCACACTGGA ACAACCCATC 
ATCCCCTCTG ACTATGTTGC CATTAAGGCT CCCATGTTTT CCTGGCCCCG ACTGAGGGAT 
GCTGATCCTA TTCTGAGATG TGAGATGGCT TCTACTGGAG AGGTGGCGTG TTTTGGTGAG 
GGCATTCATA CAGCCTTCCT AAAGGCAATG CTGTCCACAG GGTTTAAGAT ACCTCAGAAG 
GGCATTCTGA TTGGCATCCA GCAATCATTC CGTCCAAGAT TCCTTGGTGT TGCTGAGCAG 
TTACACAATG AAGGTTTCAA GCTTTTTGCC ACAGAAGCCA CATCAGACTG GCTCAACGCC 
AACAATGTTC CTGCCACCCC AGTGGCTTGG CCATCTCAGG AAGGACAGAA TCCCAGCCTC 
TCTTCCATCA GAAAGTTGAT AAGAGACGGA AGCATTGACC TAGTGATTAA CCTCCCCAAT 
AACAACACCA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT GGACAGTGGA 
ATTGCTCTGC TCACCAATTT CCAGGTGACC AAACTTTTTG CTGAGGCTGT GCAGAAAGCT 
CGTACTGTGG ACTCCAAGAG TCTGTTCCAC TACAGGCAGT ACAGCGCTGG AAAGGCAGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cps1 ( XM_017596592.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452081.1 Rattus norvegicus carbamoyl-phosphate synthase 1 (Cps1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13342
Clone ID Related Accession (Same CDS sequence)	NM_017072.2 , XM_017596592.1
Accession Version	XM_017596592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGGA TTTTGACAGC ATGCAAAGTG GTGAAGACAC TGAAGAGTGG CTTTGGTTTG 
GCCAATGTGA CTTCGAAGCG ACAGTGGGAC TTCTCTAGAC CTGGCATCAG GCTCCTTTCT 
GTGAAGGCAC AGACAGCACA CATTGTTCTG GAAGATGGAA CTAAGATGAA GGGCTACTCC 
TTTGGCCATC CCTCCTCGGT TGCTGGCGAA GTGGTTTTTA ATACTGGCTT AGGAGGGTAC 
TCGGAAGCAC TTACTGATCC TGCCTACAAG GGGCAGATCC TCACCATGGC CAACCCTATC 
ATTGGGAATG GTGGGGCCCC TGATACAACG GCACGTGATG AACTGGGACT GAATAAGTAC 
ATGGAGTCTG ATGGAATCAA GGTGGCGGGT CTGCTGGTGC TGAATTACAG TCATGACTAC 
AACCACTGGC TGGCCACCAA GAGTCTGGGT CAGTGGCTGC AGGAGGAGAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT 
GGGAAGATTG AGTTTGAGGG CCAGTCTGTG GACTTTGTGG ATCCTAATAA GCAGAATTTG 
ATTGCCGAGG TTTCAACCAA GGATGTCAAG GTGTTTGGCA AAGGAAACCC CACGAAAGTG 
GTAGCCGTGG ACTGTGGGAT AAAAAACAAT GTCATCCGCC TGCTAGTTAA GCGAGGAGCG 
GAAGTGCATT TGGTCCCCTG GAATCATGAC TTCACCCAGA TGGACTATGA CGGACTTCTG 
ATCGCTGGAG GACCTGGGAA CCCAGCTCTG GCACAGCCAC TAATTCAGAA CGTGAAGAAG 
ATTTTGGAGA GTGACCGCAA AGAGCCGTTG TTTGGAATCA GTACAGGAAA CATAATAACA 
GGATTGGCTG CTGGCGCCAA ATCCTACAAG ATGTCCATGG CCAACAGAGG ACAGAACCAA 
CCTGTTTTGA ATATCACAAA CAGACAGGCT TTCATAACTG CTCAGAATCA TGGCTATGCT 
CTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTGTTTG TCAATGTCAA TGACCAAACA 
AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC 
AGCCCGGGGC CAACAGACAC TGAGTACCTA TTTGATTCCT TCTTCTCGCT GATAAAGAAG 
GGCAAAGGCA CCACCATTAC CTCAGTTCTG CCCAAGCCAG CATTGGTGGC ATCTCGAGTC 
GAGGTTTCCA AGGTCCTTAT CCTAGGATCA GGAGGCCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CCGGATCTCA GGCTGTAAAA GCCATGAAGG AAGAAAACGT CAAAACAGTC 
CTGATGAACC CGAACATTGC ATCCGTGCAG ACCAACGAGG TGGGATTGAA GCAGGCAGAT 
GCAGTCTACT TTCTCCCTAT CACCCCCCAG TTTGTCACAG AAGTTATCAA GGCTGAACGG 
CCCGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA 
TTCAAGAGGG GTGTGCTCAA GGAATATGGT GTGAAGGTCT TGGGAACATC GGTCGAGTCC 
ATTATGGCCA CAGAAGACAG GCAGCTTTTC TCAGACAAGC TGAATGAGAT CAACGAGAAG 
ATTGCTCCTA GCTTTGCAGT GGAATCAATG GAGGATGCCT TGAAGGCAGC AGACACCATT 
GGCTACCCTG TGATGATTCG GTCTGCGTAT GCTCTGGGTG GGTTAGGCTC CGGCATCTGT 
CCCAACAAGG AGACCTTAAT GGATCTTGGC ACAAAGGCAT TTGCTATGAC CAACCAGATC 
CTGGTGGAGC GGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTTGT CCGAGATGCG 
GATGATAACT GTGTCACAGT CTGTAACATG GAGAATGTTG ACGCCATGGG TGTTCACACA 
GGTGATTCAG TTGTTGTGGC CCCGGCCCAG ACCCTCTCCA ATGCAGAGTT CCAGATGTTG 
AGACGCACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG 
TTTGCTCTTC ACCCCACTTC CATGGAATAC TGTATCATTG AAGTGAATGC CAGGCTCTCC 
AGGAGTTCTG CCCTGGCCTC CAAGGCCACT GGCTACCCAC TGGCGTTCAT CGCAGCAAAG 
ATCGCTCTAG GAATCCCACT TCCAGAAATC AAGAATGTTG TGTCTGGGAA GACCTCAGCC 
TGCTTCGAAC CTAGCCTGGA TTACATGGTG ACCAAGATTC CTCGCTGGGA CCTTGACCGT 
TTTCATGGAA CATCCAGCCG AATTGGTAGC TCAATGAAAA GTGTAGGCGA GGTCATGGCC 
ATTGGTCGCA CCTTTGAGGA AAGTTTCCAG AAGGCTCTGA GGATGTGCCA TCCATCTGTG 
GATGGGTTCA CTCCCCGTCT CCCAATGAAT AAGGAATGGC CAGCAAACCT GGATCTGAGG 
AAGGAGCTGT CTGAACCCTC CAGCACCCGC ATCTATGCCA TTGCTAAGGC CTTGGAAAAC 
AACATGTCCC TTGACGAAAT CGTGAAGCTC ACATCCATTG ACAAGTGGTT TTTGTATAAG 
ATGCGTGACA TTTTAAACAT GGATAAGACA CTGAAAGGGC TTAACAGTGA GTCTGTTACA 
GAAGAAACTC TGAGACAGGC CAAAGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGT 
TTAGGACTGA CCGAGGCTCA GACAAGAGAG CTGAGATTGA AGAAAAACAT CCACCCTTGG 
GTTAAACAGA TTGATACATT GGCTGCAGAA TACCCATCAG TGACAAATTA CCTGTATGTT 
ACCTACAATG GCCAGGAGCA CGACATCAAA TTTGATGAAC ATGGAATAAT GGTGCTGGGC 
TGTGGCCCAT ACCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACACTGC GCCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGTAACCC GGAGACTGTG 
AGCACTGACT TTGATGAGTG TGACAAACTC TACTTTGAAG AGCTGTCTTT GGAGAGAATC 
CTAGATATCT ACCACCAGGA GGCATGTAAT GGCTGCATCA TATCAGTCGG GGGCCAGATT 
CCAAACAACT TGGCGGTTCC GCTATACAAG AACGGTGTCA AGATCATGGG TACCAGTCCT 
CTGCAGATCG ATAGGGCTGA GGATCGCTCC ATCTTCTCGG CTGTCTTAGA TGAGCTGAAG 
GTGGCCCAGG CTCCTTGGAA AGCTGTTAAC ACTTTGAACG AGGCGCTGGA ATTTGCAAAC 
TCTGTGGGCT ATCCCTGCTT ACTGAGACCT TCCTATGTTT TGAGTGGGTC TGCCATGAAC 
GTGGTATTCT CTGAGGATGA GATGAAAAGG TTCCTTGAGG AGGCCACTCG AGTCTCTCAG 
GAACACCCAG TGGTGCTGAC CAAGTTTATT GAGGGGGCTC GGGAAGTGGA GATGGACGCT 
GTTGGCAAAG AAGGACGGGT CATCTCCCAT GCCATCTCTG AACATGTTGA AGATGCAGGT 
GTCCACTCAG GGGATGCCAC ACTGATGCTA CCTACGCAGA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCCAC ACGAAAGATT GCAAAGGCTT TTGCCATCTC TGGGCCATTC 
AATGTTCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGCAA TCTGAGAGCC 
TCTCGATCCT TCCCCTTTGT CTCCAAGACT CTTGGGGTGG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TCGGAGAGAG TGTTGATGAG AAGCATCTAC CCACACTGGA ACAACCCATC 
ATCCCCTCTG ACTATGTTGC CATTAAGGCT CCCATGTTTT CCTGGCCCCG ACTGAGGGAT 
GCTGATCCTA TTCTGAGATG TGAGATGGCT TCTACTGGAG AGGTGGCGTG TTTTGGTGAG 
GGCATTCATA CAGCCTTCCT AAAGGCAATG CTGTCCACAG GGTTTAAGAT ACCTCAGAAG 
GGCATTCTGA TTGGCATCCA GCAATCATTC CGTCCAAGAT TCCTTGGTGT TGCTGAGCAG 
TTACACAATG AAGGTTTCAA GCTTTTTGCC ACAGAAGCCA CATCAGACTG GCTCAACGCC 
AACAATGTTC CTGCCACCCC AGTGGCTTGG CCATCTCAGG AAGGACAGAA TCCCAGCCTC 
TCTTCCATCA GAAAGTTGAT AAGAGACGGA AGCATTGACC TAGTGATTAA CCTCCCCAAT 
AACAACACCA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT GGACAGTGGA 
ATTGCTCTGC TCACCAATTT CCAGGTGACC AAACTTTTTG CTGAGGCTGT GCAGAAAGCT 
CGTACTGTGG ACTCCAAGAG TCTGTTCCAC TACAGGCAGT ACAGCGCTGG AAAGGCAGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452081.1
CDS	162..4664
Translation	MTRILTACKVVKTLKSGFGLANVTSKRQWDFSRPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYSEALTDPAYKGQILTMANPIIGNGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSHDYNHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVKVFGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMDYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFDSFFSLIKKGKGTTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLMDLGTKAFAMTNQILVERSVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLRKELSEPSSTRIYAIAKALENNMSLDEIVKLTSIDKWFLYKMRDILNMDKTLKGLNSESVTEETLRQAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFDEHGIMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACNGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFANSVGYPCLLRPSYVLSGSAMNVVFSEDEMKRFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKEGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGVDFIDVATKVMIGESVDEKHLPTLEQPIIPSDYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVQKARTVDSKSLFHYRQYSAGKAA

Target ORF information:

RefSeq Version	XM_017596592.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carbamoyl-phosphate synthase 1 (Cps1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596592.1

ORF Insert Sequence:

ATGACGAGGA TTTTGACAGC ATGCAAAGTG GTGAAGACAC TGAAGAGTGG CTTTGGTTTG 
GCCAATGTGA CTTCGAAGCG ACAGTGGGAC TTCTCTAGAC CTGGCATCAG GCTCCTTTCT 
GTGAAGGCAC AGACAGCACA CATTGTTCTG GAAGATGGAA CTAAGATGAA GGGCTACTCC 
TTTGGCCATC CCTCCTCGGT TGCTGGCGAA GTGGTTTTTA ATACTGGCTT AGGAGGGTAC 
TCGGAAGCAC TTACTGATCC TGCCTACAAG GGGCAGATCC TCACCATGGC CAACCCTATC 
ATTGGGAATG GTGGGGCCCC TGATACAACG GCACGTGATG AACTGGGACT GAATAAGTAC 
ATGGAGTCTG ATGGAATCAA GGTGGCGGGT CTGCTGGTGC TGAATTACAG TCATGACTAC 
AACCACTGGC TGGCCACCAA GAGTCTGGGT CAGTGGCTGC AGGAGGAGAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT 
GGGAAGATTG AGTTTGAGGG CCAGTCTGTG GACTTTGTGG ATCCTAATAA GCAGAATTTG 
ATTGCCGAGG TTTCAACCAA GGATGTCAAG GTGTTTGGCA AAGGAAACCC CACGAAAGTG 
GTAGCCGTGG ACTGTGGGAT AAAAAACAAT GTCATCCGCC TGCTAGTTAA GCGAGGAGCG 
GAAGTGCATT TGGTCCCCTG GAATCATGAC TTCACCCAGA TGGACTATGA CGGACTTCTG 
ATCGCTGGAG GACCTGGGAA CCCAGCTCTG GCACAGCCAC TAATTCAGAA CGTGAAGAAG 
ATTTTGGAGA GTGACCGCAA AGAGCCGTTG TTTGGAATCA GTACAGGAAA CATAATAACA 
GGATTGGCTG CTGGCGCCAA ATCCTACAAG ATGTCCATGG CCAACAGAGG ACAGAACCAA 
CCTGTTTTGA ATATCACAAA CAGACAGGCT TTCATAACTG CTCAGAATCA TGGCTATGCT 
CTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTGTTTG TCAATGTCAA TGACCAAACA 
AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC 
AGCCCGGGGC CAACAGACAC TGAGTACCTA TTTGATTCCT TCTTCTCGCT GATAAAGAAG 
GGCAAAGGCA CCACCATTAC CTCAGTTCTG CCCAAGCCAG CATTGGTGGC ATCTCGAGTC 
GAGGTTTCCA AGGTCCTTAT CCTAGGATCA GGAGGCCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CCGGATCTCA GGCTGTAAAA GCCATGAAGG AAGAAAACGT CAAAACAGTC 
CTGATGAACC CGAACATTGC ATCCGTGCAG ACCAACGAGG TGGGATTGAA GCAGGCAGAT 
GCAGTCTACT TTCTCCCTAT CACCCCCCAG TTTGTCACAG AAGTTATCAA GGCTGAACGG 
CCCGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA 
TTCAAGAGGG GTGTGCTCAA GGAATATGGT GTGAAGGTCT TGGGAACATC GGTCGAGTCC 
ATTATGGCCA CAGAAGACAG GCAGCTTTTC TCAGACAAGC TGAATGAGAT CAACGAGAAG 
ATTGCTCCTA GCTTTGCAGT GGAATCAATG GAGGATGCCT TGAAGGCAGC AGACACCATT 
GGCTACCCTG TGATGATTCG GTCTGCGTAT GCTCTGGGTG GGTTAGGCTC CGGCATCTGT 
CCCAACAAGG AGACCTTAAT GGATCTTGGC ACAAAGGCAT TTGCTATGAC CAACCAGATC 
CTGGTGGAGC GGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTTGT CCGAGATGCG 
GATGATAACT GTGTCACAGT CTGTAACATG GAGAATGTTG ACGCCATGGG TGTTCACACA 
GGTGATTCAG TTGTTGTGGC CCCGGCCCAG ACCCTCTCCA ATGCAGAGTT CCAGATGTTG 
AGACGCACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG 
TTTGCTCTTC ACCCCACTTC CATGGAATAC TGTATCATTG AAGTGAATGC CAGGCTCTCC 
AGGAGTTCTG CCCTGGCCTC CAAGGCCACT GGCTACCCAC TGGCGTTCAT CGCAGCAAAG 
ATCGCTCTAG GAATCCCACT TCCAGAAATC AAGAATGTTG TGTCTGGGAA GACCTCAGCC 
TGCTTCGAAC CTAGCCTGGA TTACATGGTG ACCAAGATTC CTCGCTGGGA CCTTGACCGT 
TTTCATGGAA CATCCAGCCG AATTGGTAGC TCAATGAAAA GTGTAGGCGA GGTCATGGCC 
ATTGGTCGCA CCTTTGAGGA AAGTTTCCAG AAGGCTCTGA GGATGTGCCA TCCATCTGTG 
GATGGGTTCA CTCCCCGTCT CCCAATGAAT AAGGAATGGC CAGCAAACCT GGATCTGAGG 
AAGGAGCTGT CTGAACCCTC CAGCACCCGC ATCTATGCCA TTGCTAAGGC CTTGGAAAAC 
AACATGTCCC TTGACGAAAT CGTGAAGCTC ACATCCATTG ACAAGTGGTT TTTGTATAAG 
ATGCGTGACA TTTTAAACAT GGATAAGACA CTGAAAGGGC TTAACAGTGA GTCTGTTACA 
GAAGAAACTC TGAGACAGGC CAAAGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGT 
TTAGGACTGA CCGAGGCTCA GACAAGAGAG CTGAGATTGA AGAAAAACAT CCACCCTTGG 
GTTAAACAGA TTGATACATT GGCTGCAGAA TACCCATCAG TGACAAATTA CCTGTATGTT 
ACCTACAATG GCCAGGAGCA CGACATCAAA TTTGATGAAC ATGGAATAAT GGTGCTGGGC 
TGTGGCCCAT ACCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGCACACTGC GCCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGTAACCC GGAGACTGTG 
AGCACTGACT TTGATGAGTG TGACAAACTC TACTTTGAAG AGCTGTCTTT GGAGAGAATC 
CTAGATATCT ACCACCAGGA GGCATGTAAT GGCTGCATCA TATCAGTCGG GGGCCAGATT 
CCAAACAACT TGGCGGTTCC GCTATACAAG AACGGTGTCA AGATCATGGG TACCAGTCCT 
CTGCAGATCG ATAGGGCTGA GGATCGCTCC ATCTTCTCGG CTGTCTTAGA TGAGCTGAAG 
GTGGCCCAGG CTCCTTGGAA AGCTGTTAAC ACTTTGAACG AGGCGCTGGA ATTTGCAAAC 
TCTGTGGGCT ATCCCTGCTT ACTGAGACCT TCCTATGTTT TGAGTGGGTC TGCCATGAAC 
GTGGTATTCT CTGAGGATGA GATGAAAAGG TTCCTTGAGG AGGCCACTCG AGTCTCTCAG 
GAACACCCAG TGGTGCTGAC CAAGTTTATT GAGGGGGCTC GGGAAGTGGA GATGGACGCT 
GTTGGCAAAG AAGGACGGGT CATCTCCCAT GCCATCTCTG AACATGTTGA AGATGCAGGT 
GTCCACTCAG GGGATGCCAC ACTGATGCTA CCTACGCAGA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCCAC ACGAAAGATT GCAAAGGCTT TTGCCATCTC TGGGCCATTC 
AATGTTCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGCAA TCTGAGAGCC 
TCTCGATCCT TCCCCTTTGT CTCCAAGACT CTTGGGGTGG ACTTCATTGA TGTGGCCACC 
AAGGTGATGA TCGGAGAGAG TGTTGATGAG AAGCATCTAC CCACACTGGA ACAACCCATC 
ATCCCCTCTG ACTATGTTGC CATTAAGGCT CCCATGTTTT CCTGGCCCCG ACTGAGGGAT 
GCTGATCCTA TTCTGAGATG TGAGATGGCT TCTACTGGAG AGGTGGCGTG TTTTGGTGAG 
GGCATTCATA CAGCCTTCCT AAAGGCAATG CTGTCCACAG GGTTTAAGAT ACCTCAGAAG 
GGCATTCTGA TTGGCATCCA GCAATCATTC CGTCCAAGAT TCCTTGGTGT TGCTGAGCAG 
TTACACAATG AAGGTTTCAA GCTTTTTGCC ACAGAAGCCA CATCAGACTG GCTCAACGCC 
AACAATGTTC CTGCCACCCC AGTGGCTTGG CCATCTCAGG AAGGACAGAA TCCCAGCCTC 
TCTTCCATCA GAAAGTTGAT AAGAGACGGA AGCATTGACC TAGTGATTAA CCTCCCCAAT 
AACAACACCA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT GGACAGTGGA 
ATTGCTCTGC TCACCAATTT CCAGGTGACC AAACTTTTTG CTGAGGCTGT GCAGAAAGCT 
CGTACTGTGG ACTCCAAGAG TCTGTTCCAC TACAGGCAGT ACAGCGCTGG AAAGGCAGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conditional disruption of hepatic carbamoyl phosphate synthetase 1 in mice results in hyperammonemia without orotic aciduria and can be corrected by liver-directed gene therapy.
Molecular genetics and metabolism124(4)243-253(2018 08)
Khoja S,Nitzahn M,Hermann K,Truong B,Borzone R,Willis B,Rudd M,Palmer DJ,Ng P,Brunetti-Pierri N,Lipshutz GS

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 04)
Martin J,Balmer ML,Rajendran S,Maurhofer O,Dufour JF,St-Pierre MV

Isoaspartate, carbamoyl phosphate synthase-1, and carbonic anhydrase-III as biomarkers of liver injury.
Biochemical and biophysical research communications458(3)626-631(2015 Mar)
Carter WG,Vigneswara V,Newlaczyl A,Wayne D,Ahmed B,Saddington S,Brewer C,Raut N,Gerdes HK,Erdozain AM,Tooth D,Bolt EL,Osna NA,Tuma DJ,Kharbanda KK

Protein tyrosine nitration of mitochondrial carbamoyl phosphate synthetase 1 and its functional consequences.
Biochemical and biophysical research communications420(1)54-60(2012 Mar)
Takakusa H,Mohar I,Kavanagh TJ,Kelly EJ,Kaspera R,Nelson SD

Molecular defects in human carbamoy phosphate synthetase I: mutational spectrum, diagnostic and protein structure considerations.
Human mutation32(6)579-89(2011 Jun)
Häberle J,Shchelochkov OA,Wang J,Katsonis P,Hall L,Reiss S,Eeds A,Willis A,Yadav M,Summar S,,Lichtarge O,Rubio V,Wong LJ,Summar M

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Cps1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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