Emc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Emc1
Entrez Gene ID	362643
Full Name	ER membrane protein complex subunit 1
Synonyms	RGD1310427
General protein information	Preferred Names ER membrane protein complex subunit 1 Names ER membrane protein complex subunit 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108690.1	NP_001102160.1	ER membrane protein complex subunit 1 precursor
XM_006239245.3	XP_006239307.1	ER membrane protein complex subunit 1 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	EMC1	XP_001091700.1
Canis lupus familiaris (dog)	EMC1	XP_005617944.1
Drosophila melanogaster (fruit fly)	CG2943	NP_649747.1
Mus musculus (house mouse)	Emc1	NP_666269.2
Arabidopsis thaliana (thale cress)	AT5G11560	NP_196717.3
Oryza sativa (rice)	Os05g0230600	NP_001054975.1
Bos taurus (cattle)	EMC1	XP_005203411.1
Danio rerio (zebrafish)	emc1	XP_693770.3
Caenorhabditis elegans (roundworm)	H17B01.4	NP_493980.1
Gallus gallus (chicken)	EMC1	NP_001012856.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003647	XP_562511.3
Homo sapiens (human)	EMC1	NP_055862.1
Pan troglodytes (chimpanzee)	EMC1	XP_001162981.2
Rattus norvegicus (Norway rat)	Emc1	NP_001102160.1
Xenopus tropicalis (tropical clawed frog)	emc1	XP_002943252.2

Gene Ontology
Bibliography

Related articles in PubMed

ZMPSTE24 defends against influenza and other pathogenic viruses.
Fu B, Wang L, Li S, Dorf ME
The Journal of experimental medicine214(4)919-929(2017 04)

Contribution of calumin to embryogenesis through participation in the endoplasmic reticulum-associated degradation activity.
Yamamoto S, Yamazaki T, Komazaki S, Yamashita T, Osaki M, Matsubayashi M, Kidoya H, Takakura N, Yamazaki D, Kakizawa S
Developmental biology393(1)33-43(2014 Sep)

Defining human ERAD networks through an integrative mapping strategy.
Christianson JC, Olzmann JA, Shaler TA, Sowa ME, Bennett EJ, Richter CM, Tyler RE, Greenblatt EJ, Harper JW, Kopito RR
Nature cell biology14(1)93-105(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Emc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Emc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08137	NM_001108690.1 Latest version!	Rattus norvegicus ER membrane protein complex subunit 1 (Emc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa28812	XM_006239245.3 Latest version!	Rattus norvegicus ER membrane protein complex subunit 1 (Emc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Emc1 ( NM_001108690.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102160.1 Rattus norvegicus ER membrane protein complex subunit 1 (Emc1), mRNA.

Price & Availability↑

CloneID	ORa08137
Clone ID Related Accession (Same CDS sequence)	NM_001108690.1
Accession Version	NM_001108690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	ER membrane protein complex subunit 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473968.1. On or before Oct 4, 2007 this sequence version replaced XM_342959.3, XM_001071619.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG CTGTGGAGTC CTGGTTCTGC CTCTGTGCTG TGCTACTTGT CCCTGCGGCC 
GCAGTCTACG AAGACCAAGT GGGCAAGTTT GACTGGAGAC AGCAGTACGT TGGGAAGCTC 
AAGTTTGCCT CCTTGGAGTT CTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCATTGAA TTCTCGGACT GGAGAGATTT TGTGGCGCCA TGTTGACAAG 
GGTACAGCAG AAGGGGCTGT GGATGCCATG CTGGTCCATG GGCAAGATGC AATCACTGTA 
TCCAATGGAG GGCGCATCAT GCGTTCTTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACTCTGG ACAGTGGCAG TTTCCAGGCA CTCGGGCTGG TGGGCCTCCA GGAGACAGTG 
AGGTACGTTG CGGTTCTGAA GAAGACGACC CTCACTCTCC ATCACCTCTC TAGTGGGCAC 
CTGAAGTGGG TGGAGCATCT GCCAGAAAGT GACAGCATCC TCTACCAGAT GGTGTATTCC 
TACGGCTCCG GGGTGGTGTG GGCCCTTGGC ATCGTTCCCT TCAGCCATGT GAACATCGTC 
AAGTTTAACG TGGAGGATGG AGAGATTGTT CAGCAGGTCA GGGTTTGGAC CCCCTGGCTG 
CAGCACCTGA CTGGAGCCTG TGGTGTTGTA GACGAGGCTG TCCTGGTATG CCCAGACCCC 
AGCTCACATT CGCTCCACAC TTTGGCCCTG GAGACGGAGT GGGAACTGCG GCAGATCCCC 
CTGCAGTCTC CTGACTTAGA ATTTGGAAGT GGATTCCAGC CCCAAGTGCT GCCCACGCAG 
CCCAGCCCAG TGGCTCCTTC TCGGGCCCAG TTCTTCCTGC AGTTGTCCCC GGGCCACTAT 
GCTCTACTGC ACTACCACCA CGGCGCCGTG ACTCTGCTCA AAAACTTTCC CCAGGCCACA 
CTTGTCAGCT TTGCTACCAC AGGGGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGCACT 
GAAGTGCAAA AACCTGGCAG TGCTGGAGAT GGGTCTGTGG CGAGCTTCCC TGAGACATCT 
GGTGCACAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT GTACTTAGTA 
GAGACAGGTC GGCGGCTACT AGACACTTCG ATTTCCTTCA GCTTGGAGCA GAAGGGCACT 
CGACCTGAGC AGCTGTACGT CCAGGTGTTC TTGAAGAAGG ATGACTCGGT GGGCTACCGG 
GCCTTGGTGC AGACACAGGA CCATCTGCAG CTGTTCCTGC AGCAGCTTGC CGGGAAGGTG 
GTGTTGTGGA GCCGAGAGGA GTCCCTGGCA GAAGTCGTGT GCCTGGAGAT GGTGGATCTC 
CCCCTGACTG GGGCACAGGC TGAGCTGGAA GGAGAATTTG GCAAGAAGGC AGATGGCTTG 
CTGGGGATGT TCCTGAAACG CCTGTCTTCC CAGCTCATCC TGCTCCAGGC CTGGACTTCC 
CACCTCTGGA AAATGTTCTA CGACGCTCGG AAGCCCCGAA GCCAGATTAA GAATGAGATC 
AACATCGACA CCTTGGCTAG AGATGAGTTC AACCTGCAGA AAATGATGGT GATGGTAACA 
GCCTCAGGCA AGCTCTTTGG CATCGAGAGC AGCTCTGGCA CCATTCTGTG GAAACAGTAT 
CTGCCCAATG TTAAGCCGGA CTCCTCCTTT AAGCTGATGG TCCAGAGGAC TACTGCCCAT 
TTCCCCCACC CGCCCCAGTG CACGCTGCTG GTGAAGGACA AGGAAACAGG AATGAGCTCT 
CTGTTTGTCT TCAACCCCAT TTTTGGCAAG TGGAGTCAGG TAGCTCCCCC AGTGTTGAAG 
CGCCCCATCC TGCAGTCCTT GCTTCTCCCG GTCATGGATC AAGACTATGC CAAGGTGTTG 
CTGTTGGTGG ATGATGAATA CAAGGTCACA GCTTTCCCAG CTACTCGGAA TGTTTTGCGA 
CAGCTACATG AACTTGCCCC CTCCATCTTC TTCTACTTGG TGGATGCCGA GCAGGGGCGG 
CTCAGCGGGT ATCGGCTTCG CAAGGATCTC ACTACAGAAC TGAGTTGGGA GCTGATCATC 
CCCCCAGAAG TGCAACGGGT CGTCCAGGTG AAGGGGAAGC GCAGCAGTGA GCACGTGCAC 
TCCCAGGGCC GGGTGATGGG GGATCGAAGC GTGCTCTACA AGAGTCTGAA CCCCAACTTG 
CTGGCTGTGG TGACGGAGAG CACGGATGTC CATCACGAGC GCACCTTTAT TGGCATCTTC 
CTCATTGACG GTGTCACTGG CCGCATTATC CACTCCTCTG TGCAGAAGAA GGCTAGGGGC 
CCTGTCCACC TTGTGCATTC AGAGAACTGG GTGGTGTACC AGTACTGGAA CTCTAAGGCT 
CGGCGCAATG AGCTGACGGC CCTGGAGCTC TACGAGGGCA CTGAGCAGTA CAATGCCACT 
GCCTTCAGCT CCCTAGACCG ACCCCAGCTG CCCCAGGTCC TCCAGCAGTC CTACATCTTC 
CCTTCCTCCA TCAGTGCCAT GGAGGCTACC ATCACCGAGC GGGGCATCAC CAGCCGGCAC 
TTGCTCATTG GGCTGCCTTC TGGAGCAATC CTTTCCCTCC CCAAAGCCTT GCTGGATCCC 
CGGCGCCCAG AGATCCCAAC AGAACAAAGC AGAGAAGAGA ACCTGATCCC GTATTCCCCA 
GATGTCCAGG TCCATGCAGA GCGATTCATC AACTATAACC AGACAGTCTC TCGAATGCGA 
GGCATCTATA CAGCGCCCTC AGGCCTGGAG TCCACATGTT TGGTTGTGGC CTATGGTCTG 
GATATTTACC AAACTCGAGT TTACCCATCC AAGCAGTTCG ATGTCCTGAA AGATGACTAT 
GACTATGTGC TCATCAGCAG TGTCCTTTTT GGCCTGGTTT TTGCCACCAT GATCACGAAG 
AGGCTGGCAC AGGTGAAACT CCTCAATCGA GCCTGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102160.1
CDS	26..3007
Translation	MAAAVESWFCLCAVLLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLVHGQDAITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQETVRYVAVLKKTTLTLHHLSSGHLKWVEHLPESDSILYQMVYSYGSGVVWALGIVPFSHVNIVKFNVEDGEIVQQVRVWTPWLQHLTGACGVVDEAVLVCPDPSSHSLHTLALETEWELRQIPLQSPDLEFGSGFQPQVLPTQPSPVAPSRAQFFLQLSPGHYALLHYHHGAVTLLKNFPQATLVSFATTGEKTVAAVMTCRTEVQKPGSAGDGSVASFPETSGAQDSLACFNQTYTINLYLVETGRRLLDTSISFSLEQKGTRPEQLYVQVFLKKDDSVGYRALVQTQDHLQLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKETGMSSLFVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLVDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLSGYRLRKDLTTELSWELIIPPEVQRVVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGIFLIDGVTGRIIHSSVQKKARGPVHLVHSENWVVYQYWNSKARRNELTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQVHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001108690.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ER membrane protein complex subunit 1 (Emc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108690.1

ORF Insert Sequence:

ATGGCGGCGG CTGTGGAGTC CTGGTTCTGC CTCTGTGCTG TGCTACTTGT CCCTGCGGCC 
GCAGTCTACG AAGACCAAGT GGGCAAGTTT GACTGGAGAC AGCAGTACGT TGGGAAGCTC 
AAGTTTGCCT CCTTGGAGTT CTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCATTGAA TTCTCGGACT GGAGAGATTT TGTGGCGCCA TGTTGACAAG 
GGTACAGCAG AAGGGGCTGT GGATGCCATG CTGGTCCATG GGCAAGATGC AATCACTGTA 
TCCAATGGAG GGCGCATCAT GCGTTCTTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACTCTGG ACAGTGGCAG TTTCCAGGCA CTCGGGCTGG TGGGCCTCCA GGAGACAGTG 
AGGTACGTTG CGGTTCTGAA GAAGACGACC CTCACTCTCC ATCACCTCTC TAGTGGGCAC 
CTGAAGTGGG TGGAGCATCT GCCAGAAAGT GACAGCATCC TCTACCAGAT GGTGTATTCC 
TACGGCTCCG GGGTGGTGTG GGCCCTTGGC ATCGTTCCCT TCAGCCATGT GAACATCGTC 
AAGTTTAACG TGGAGGATGG AGAGATTGTT CAGCAGGTCA GGGTTTGGAC CCCCTGGCTG 
CAGCACCTGA CTGGAGCCTG TGGTGTTGTA GACGAGGCTG TCCTGGTATG CCCAGACCCC 
AGCTCACATT CGCTCCACAC TTTGGCCCTG GAGACGGAGT GGGAACTGCG GCAGATCCCC 
CTGCAGTCTC CTGACTTAGA ATTTGGAAGT GGATTCCAGC CCCAAGTGCT GCCCACGCAG 
CCCAGCCCAG TGGCTCCTTC TCGGGCCCAG TTCTTCCTGC AGTTGTCCCC GGGCCACTAT 
GCTCTACTGC ACTACCACCA CGGCGCCGTG ACTCTGCTCA AAAACTTTCC CCAGGCCACA 
CTTGTCAGCT TTGCTACCAC AGGGGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGCACT 
GAAGTGCAAA AACCTGGCAG TGCTGGAGAT GGGTCTGTGG CGAGCTTCCC TGAGACATCT 
GGTGCACAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT GTACTTAGTA 
GAGACAGGTC GGCGGCTACT AGACACTTCG ATTTCCTTCA GCTTGGAGCA GAAGGGCACT 
CGACCTGAGC AGCTGTACGT CCAGGTGTTC TTGAAGAAGG ATGACTCGGT GGGCTACCGG 
GCCTTGGTGC AGACACAGGA CCATCTGCAG CTGTTCCTGC AGCAGCTTGC CGGGAAGGTG 
GTGTTGTGGA GCCGAGAGGA GTCCCTGGCA GAAGTCGTGT GCCTGGAGAT GGTGGATCTC 
CCCCTGACTG GGGCACAGGC TGAGCTGGAA GGAGAATTTG GCAAGAAGGC AGATGGCTTG 
CTGGGGATGT TCCTGAAACG CCTGTCTTCC CAGCTCATCC TGCTCCAGGC CTGGACTTCC 
CACCTCTGGA AAATGTTCTA CGACGCTCGG AAGCCCCGAA GCCAGATTAA GAATGAGATC 
AACATCGACA CCTTGGCTAG AGATGAGTTC AACCTGCAGA AAATGATGGT GATGGTAACA 
GCCTCAGGCA AGCTCTTTGG CATCGAGAGC AGCTCTGGCA CCATTCTGTG GAAACAGTAT 
CTGCCCAATG TTAAGCCGGA CTCCTCCTTT AAGCTGATGG TCCAGAGGAC TACTGCCCAT 
TTCCCCCACC CGCCCCAGTG CACGCTGCTG GTGAAGGACA AGGAAACAGG AATGAGCTCT 
CTGTTTGTCT TCAACCCCAT TTTTGGCAAG TGGAGTCAGG TAGCTCCCCC AGTGTTGAAG 
CGCCCCATCC TGCAGTCCTT GCTTCTCCCG GTCATGGATC AAGACTATGC CAAGGTGTTG 
CTGTTGGTGG ATGATGAATA CAAGGTCACA GCTTTCCCAG CTACTCGGAA TGTTTTGCGA 
CAGCTACATG AACTTGCCCC CTCCATCTTC TTCTACTTGG TGGATGCCGA GCAGGGGCGG 
CTCAGCGGGT ATCGGCTTCG CAAGGATCTC ACTACAGAAC TGAGTTGGGA GCTGATCATC 
CCCCCAGAAG TGCAACGGGT CGTCCAGGTG AAGGGGAAGC GCAGCAGTGA GCACGTGCAC 
TCCCAGGGCC GGGTGATGGG GGATCGAAGC GTGCTCTACA AGAGTCTGAA CCCCAACTTG 
CTGGCTGTGG TGACGGAGAG CACGGATGTC CATCACGAGC GCACCTTTAT TGGCATCTTC 
CTCATTGACG GTGTCACTGG CCGCATTATC CACTCCTCTG TGCAGAAGAA GGCTAGGGGC 
CCTGTCCACC TTGTGCATTC AGAGAACTGG GTGGTGTACC AGTACTGGAA CTCTAAGGCT 
CGGCGCAATG AGCTGACGGC CCTGGAGCTC TACGAGGGCA CTGAGCAGTA CAATGCCACT 
GCCTTCAGCT CCCTAGACCG ACCCCAGCTG CCCCAGGTCC TCCAGCAGTC CTACATCTTC 
CCTTCCTCCA TCAGTGCCAT GGAGGCTACC ATCACCGAGC GGGGCATCAC CAGCCGGCAC 
TTGCTCATTG GGCTGCCTTC TGGAGCAATC CTTTCCCTCC CCAAAGCCTT GCTGGATCCC 
CGGCGCCCAG AGATCCCAAC AGAACAAAGC AGAGAAGAGA ACCTGATCCC GTATTCCCCA 
GATGTCCAGG TCCATGCAGA GCGATTCATC AACTATAACC AGACAGTCTC TCGAATGCGA 
GGCATCTATA CAGCGCCCTC AGGCCTGGAG TCCACATGTT TGGTTGTGGC CTATGGTCTG 
GATATTTACC AAACTCGAGT TTACCCATCC AAGCAGTTCG ATGTCCTGAA AGATGACTAT 
GACTATGTGC TCATCAGCAG TGTCCTTTTT GGCCTGGTTT TTGCCACCAT GATCACGAAG 
AGGCTGGCAC AGGTGAAACT CCTCAATCGA GCCTGGCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emc1 ( XM_006239245.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239307.1 Rattus norvegicus ER membrane protein complex subunit 1 (Emc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28812
Clone ID Related Accession (Same CDS sequence)	XM_006239245.3
Accession Version	XM_006239245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CTGTGGAGTC CTGGTTCTGC CTCTGTGCTG TGCTACTTGT CCCTGCGGCC 
GCAGTCTACG AAGACCAAGT GGGCAAGTTT GACTGGAGAC AGCAGTACGT TGGGAAGCTC 
AAGTTTGCCT CCTTGGAGTT CTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCATTGAA TTCTCGGACT GGAGAGATTT TGTGGCGCCA TGTTGACAAG 
GGTACAGCAG AAGGGGCTGT GGATGCCATG CTGGTCCATG GGCAAGATGC AATCACTGTA 
TCCAATGGAG GGCGCATCAT GCGTTCTTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACTCTGG ACAGTGGCAG TTTCCAGGCA CTCGGGCTGG TGGGCCTCCA GGAGACAGTG 
AGGTACGTTG CGGTTCTGAA GAAGACGACC CTCACTCTCC ATCACCTCTC TAGTGGGCAC 
CTGAAGTGGG TGGAGCATCT GCCAGAAAGT GACAGCATCC TCTACCAGAT GGTGTATTCC 
TACGGCTCCG GGGTGGTGTG GGCCCTTGGC ATCGTTCCCT TCAGCCATGT GAACATCGTC 
AAGTTTAACG TGGAGGATGG AGAGATTGTT CAGCAGGTCA GGGTTTGGAC CCCCTGGCTG 
CAGCACCTGA CTGGAGCCTG TGGTGTTGTA GACGAGGCTG TCCTGGTATG CCCAGACCCC 
AGCTCACATT CGCTCCACAC TTTGGCCCTG GAGACGGAGT GGGAACTGCG GCAGATCCCC 
CTGCAGTCTC CTGACTTAGA ATTTGGAAGT GGATTCCAGC CCCAAGTGCT GCCCACGCAG 
CCCAGCCCAG TGGCTCCTTC TCGGGCCCAG TTCTTCCTGC AGTTGTCCCC GGGCCACTAT 
GCTCTACTGC ACTACCACCA CGGCGCCGTG ACTCTGCTCA AAAACTTTCC CCAGGCCACA 
CTTGTCAGCT TTGCTACCAC AGGGGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGCACT 
GAAGTGCAAA AACCTGGCAG TGCTGGAGAT GGGTCTGTGG CGAGCTTCCC TGAGACATCT 
GGTGCACAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT GTACTTAGTA 
GAGACAGGTC GGCGGCTACT AGACACTTCG ATTTCCTTCA GCTTGGAGCA GAAGGGCACT 
CGACCTGAGC AGCTGTACGT CCAGGTGTTC TTGAAGAAGG ATGACTCGGT GGGCTACCGG 
GCCTTGGTGC AGACACAGGA CCATCTGCAG CTGTTCCTGC AGCAGCTTGC CGGGAAGGTG 
GTGTTGTGGA GCCGAGAGGA GTCCCTGGCA GAAGTCGTGT GCCTGGAGAT GGTGGATCTC 
CCCCTGACTG GGGCACAGGC TGAGCTGGAA GGAGAATTTG GCAAGAAGGC AGCGATTCAA 
GATGGCTTGC TGGGGATGTT CCTGAAACGC CTGTCTTCCC AGCTCATCCT GCTCCAGGCC 
TGGACTTCCC ACCTCTGGAA AATGTTCTAC GACGCTCGGA AGCCCCGAAG CCAGATTAAG 
AATGAGATCA ACATCGACAC CTTGGCTAGA GATGAGTTCA ACCTGCAGAA AATGATGGTG 
ATGGTAACAG CCTCAGGCAA GCTCTTTGGC ATCGAGAGCA GCTCTGGCAC CATTCTGTGG 
AAACAGTATC TGCCCAATGT TAAGCCGGAC TCCTCCTTTA AGCTGATGGT CCAGAGGACT 
ACTGCCCATT TCCCCCACCC GCCCCAGTGC ACGCTGCTGG TGAAGGACAA GGAAACAGGA 
ATGAGCTCTC TGTTTGTCTT CAACCCCATT TTTGGCAAGT GGAGTCAGGT AGCTCCCCCA 
GTGTTGAAGC GCCCCATCCT GCAGTCCTTG CTTCTCCCGG TCATGGATCA AGACTATGCC 
AAGGTGTTGC TGTTGGTGGA TGATGAATAC AAGGTCACAG CTTTCCCAGC TACTCGGAAT 
GTTTTGCGAC AGCTACATGA ACTTGCCCCC TCCATCTTCT TCTACTTGGT GGATGCCGAG 
CAGGGGCGGC TCAGCGGGTA TCGGCTTCGC AAGGATCTCA CTACAGAACT GAGTTGGGAG 
CTGATCATCC CCCCAGAAGT GCAACGGGTC GTCCAGGTGA AGGGGAAGCG CAGCAGTGAG 
CACGTGCACT CCCAGGGCCG GGTGATGGGG GATCGAAGCG TGCTCTACAA GAGTCTGAAC 
CCCAACTTGC TGGCTGTGGT GACGGAGAGC ACGGATGTCC ATCACGAGCG CACCTTTATT 
GGCATCTTCC TCATTGACGG TGTCACTGGC CGCATTATCC ACTCCTCTGT GCAGAAGAAG 
GCTAGGGGCC CTGTCCACCT TGTGCATTCA GAGAACTGGG TGGTGTACCA GTACTGGAAC 
TCTAAGGCTC GGCGCAATGA GCTGACGGCC CTGGAGCTCT ACGAGGGCAC TGAGCAGTAC 
AATGCCACTG CCTTCAGCTC CCTAGACCGA CCCCAGCTGC CCCAGGTCCT CCAGCAGTCC 
TACATCTTCC CTTCCTCCAT CAGTGCCATG GAGGCTACCA TCACCGAGCG GGGCATCACC 
AGCCGGCACT TGCTCATTGG GCTGCCTTCT GGAGCAATCC TTTCCCTCCC CAAAGCCTTG 
CTGGATCCCC GGCGCCCAGA GATCCCAACA GAACAAAGCA GAGAAGAGAA CCTGATCCCG 
TATTCCCCAG ATGTCCAGGT CCATGCAGAG CGATTCATCA ACTATAACCA GACAGTCTCT 
CGAATGCGAG GCATCTATAC AGCGCCCTCA GGCCTGGAGT CCACATGTTT GGTTGTGGCC 
TATGGTCTGG ATATTTACCA AACTCGAGTT TACCCATCCA AGCAGTTCGA TGTCCTGAAA 
GATGACTATG ACTATGTGCT CATCAGCAGT GTCCTTTTTG GCCTGGTTTT TGCCACCATG 
ATCACGAAGA GGCTGGCACA GGTGAAACTC CTCAATCGAG CCTGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239307.1
CDS	20..3010
Translation	MAAAVESWFCLCAVLLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLVHGQDAITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQETVRYVAVLKKTTLTLHHLSSGHLKWVEHLPESDSILYQMVYSYGSGVVWALGIVPFSHVNIVKFNVEDGEIVQQVRVWTPWLQHLTGACGVVDEAVLVCPDPSSHSLHTLALETEWELRQIPLQSPDLEFGSGFQPQVLPTQPSPVAPSRAQFFLQLSPGHYALLHYHHGAVTLLKNFPQATLVSFATTGEKTVAAVMTCRTEVQKPGSAGDGSVASFPETSGAQDSLACFNQTYTINLYLVETGRRLLDTSISFSLEQKGTRPEQLYVQVFLKKDDSVGYRALVQTQDHLQLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKETGMSSLFVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLVDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLSGYRLRKDLTTELSWELIIPPEVQRVVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGIFLIDGVTGRIIHSSVQKKARGPVHLVHSENWVVYQYWNSKARRNELTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQVHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_006239245.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ER membrane protein complex subunit 1 (Emc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239245.3

ORF Insert Sequence:

ATGGCGGCGG CTGTGGAGTC CTGGTTCTGC CTCTGTGCTG TGCTACTTGT CCCTGCGGCC 
GCAGTCTACG AAGACCAAGT GGGCAAGTTT GACTGGAGAC AGCAGTACGT TGGGAAGCTC 
AAGTTTGCCT CCTTGGAGTT CTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCATTGAA TTCTCGGACT GGAGAGATTT TGTGGCGCCA TGTTGACAAG 
GGTACAGCAG AAGGGGCTGT GGATGCCATG CTGGTCCATG GGCAAGATGC AATCACTGTA 
TCCAATGGAG GGCGCATCAT GCGTTCTTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACTCTGG ACAGTGGCAG TTTCCAGGCA CTCGGGCTGG TGGGCCTCCA GGAGACAGTG 
AGGTACGTTG CGGTTCTGAA GAAGACGACC CTCACTCTCC ATCACCTCTC TAGTGGGCAC 
CTGAAGTGGG TGGAGCATCT GCCAGAAAGT GACAGCATCC TCTACCAGAT GGTGTATTCC 
TACGGCTCCG GGGTGGTGTG GGCCCTTGGC ATCGTTCCCT TCAGCCATGT GAACATCGTC 
AAGTTTAACG TGGAGGATGG AGAGATTGTT CAGCAGGTCA GGGTTTGGAC CCCCTGGCTG 
CAGCACCTGA CTGGAGCCTG TGGTGTTGTA GACGAGGCTG TCCTGGTATG CCCAGACCCC 
AGCTCACATT CGCTCCACAC TTTGGCCCTG GAGACGGAGT GGGAACTGCG GCAGATCCCC 
CTGCAGTCTC CTGACTTAGA ATTTGGAAGT GGATTCCAGC CCCAAGTGCT GCCCACGCAG 
CCCAGCCCAG TGGCTCCTTC TCGGGCCCAG TTCTTCCTGC AGTTGTCCCC GGGCCACTAT 
GCTCTACTGC ACTACCACCA CGGCGCCGTG ACTCTGCTCA AAAACTTTCC CCAGGCCACA 
CTTGTCAGCT TTGCTACCAC AGGGGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGCACT 
GAAGTGCAAA AACCTGGCAG TGCTGGAGAT GGGTCTGTGG CGAGCTTCCC TGAGACATCT 
GGTGCACAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT GTACTTAGTA 
GAGACAGGTC GGCGGCTACT AGACACTTCG ATTTCCTTCA GCTTGGAGCA GAAGGGCACT 
CGACCTGAGC AGCTGTACGT CCAGGTGTTC TTGAAGAAGG ATGACTCGGT GGGCTACCGG 
GCCTTGGTGC AGACACAGGA CCATCTGCAG CTGTTCCTGC AGCAGCTTGC CGGGAAGGTG 
GTGTTGTGGA GCCGAGAGGA GTCCCTGGCA GAAGTCGTGT GCCTGGAGAT GGTGGATCTC 
CCCCTGACTG GGGCACAGGC TGAGCTGGAA GGAGAATTTG GCAAGAAGGC AGCGATTCAA 
GATGGCTTGC TGGGGATGTT CCTGAAACGC CTGTCTTCCC AGCTCATCCT GCTCCAGGCC 
TGGACTTCCC ACCTCTGGAA AATGTTCTAC GACGCTCGGA AGCCCCGAAG CCAGATTAAG 
AATGAGATCA ACATCGACAC CTTGGCTAGA GATGAGTTCA ACCTGCAGAA AATGATGGTG 
ATGGTAACAG CCTCAGGCAA GCTCTTTGGC ATCGAGAGCA GCTCTGGCAC CATTCTGTGG 
AAACAGTATC TGCCCAATGT TAAGCCGGAC TCCTCCTTTA AGCTGATGGT CCAGAGGACT 
ACTGCCCATT TCCCCCACCC GCCCCAGTGC ACGCTGCTGG TGAAGGACAA GGAAACAGGA 
ATGAGCTCTC TGTTTGTCTT CAACCCCATT TTTGGCAAGT GGAGTCAGGT AGCTCCCCCA 
GTGTTGAAGC GCCCCATCCT GCAGTCCTTG CTTCTCCCGG TCATGGATCA AGACTATGCC 
AAGGTGTTGC TGTTGGTGGA TGATGAATAC AAGGTCACAG CTTTCCCAGC TACTCGGAAT 
GTTTTGCGAC AGCTACATGA ACTTGCCCCC TCCATCTTCT TCTACTTGGT GGATGCCGAG 
CAGGGGCGGC TCAGCGGGTA TCGGCTTCGC AAGGATCTCA CTACAGAACT GAGTTGGGAG 
CTGATCATCC CCCCAGAAGT GCAACGGGTC GTCCAGGTGA AGGGGAAGCG CAGCAGTGAG 
CACGTGCACT CCCAGGGCCG GGTGATGGGG GATCGAAGCG TGCTCTACAA GAGTCTGAAC 
CCCAACTTGC TGGCTGTGGT GACGGAGAGC ACGGATGTCC ATCACGAGCG CACCTTTATT 
GGCATCTTCC TCATTGACGG TGTCACTGGC CGCATTATCC ACTCCTCTGT GCAGAAGAAG 
GCTAGGGGCC CTGTCCACCT TGTGCATTCA GAGAACTGGG TGGTGTACCA GTACTGGAAC 
TCTAAGGCTC GGCGCAATGA GCTGACGGCC CTGGAGCTCT ACGAGGGCAC TGAGCAGTAC 
AATGCCACTG CCTTCAGCTC CCTAGACCGA CCCCAGCTGC CCCAGGTCCT CCAGCAGTCC 
TACATCTTCC CTTCCTCCAT CAGTGCCATG GAGGCTACCA TCACCGAGCG GGGCATCACC 
AGCCGGCACT TGCTCATTGG GCTGCCTTCT GGAGCAATCC TTTCCCTCCC CAAAGCCTTG 
CTGGATCCCC GGCGCCCAGA GATCCCAACA GAACAAAGCA GAGAAGAGAA CCTGATCCCG 
TATTCCCCAG ATGTCCAGGT CCATGCAGAG CGATTCATCA ACTATAACCA GACAGTCTCT 
CGAATGCGAG GCATCTATAC AGCGCCCTCA GGCCTGGAGT CCACATGTTT GGTTGTGGCC 
TATGGTCTGG ATATTTACCA AACTCGAGTT TACCCATCCA AGCAGTTCGA TGTCCTGAAA 
GATGACTATG ACTATGTGCT CATCAGCAGT GTCCTTTTTG GCCTGGTTTT TGCCACCATG 
ATCACGAAGA GGCTGGCACA GGTGAAACTC CTCAATCGAG CCTGGCGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZMPSTE24 defends against influenza and other pathogenic viruses.
The Journal of experimental medicine214(4)919-929(2017 04)
Fu B,Wang L,Li S,Dorf ME

Contribution of calumin to embryogenesis through participation in the endoplasmic reticulum-associated degradation activity.
Developmental biology393(1)33-43(2014 Sep)
Yamamoto S,Yamazaki T,Komazaki S,Yamashita T,Osaki M,Matsubayashi M,Kidoya H,Takakura N,Yamazaki D,Kakizawa S

Defining human ERAD networks through an integrative mapping strategy.
Nature cell biology14(1)93-105(2011 Nov)
Christianson JC,Olzmann JA,Shaler TA,Sowa ME,Bennett EJ,Richter CM,Tyler RE,Greenblatt EJ,Harper JW,Kopito RR

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Emc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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