Emc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Emc1
Entrez Gene ID	230866
Full Name	ER membrane protein complex subunit 1
Synonyms	2700016F22Rik
General protein information	Preferred Names ER membrane protein complex subunit 1 Names ER membrane protein complex subunit 1 novel DUF1620 domain containing protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_146157.4	NP_666269.2	ER membrane protein complex subunit 1 isoform 1 precursor
NM_001039200.2	NP_001034289.1	ER membrane protein complex subunit 1 isoform 2 precursor

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	EMC1	XP_001091700.1
Canis lupus familiaris (dog)	EMC1	XP_005617944.1
Drosophila melanogaster (fruit fly)	CG2943	NP_649747.1
Mus musculus (house mouse)	Emc1	NP_666269.2
Arabidopsis thaliana (thale cress)	AT5G11560	NP_196717.3
Oryza sativa (rice)	Os05g0230600	NP_001054975.1
Bos taurus (cattle)	EMC1	XP_005203411.1
Danio rerio (zebrafish)	emc1	XP_693770.3
Caenorhabditis elegans (roundworm)	H17B01.4	NP_493980.1
Gallus gallus (chicken)	EMC1	NP_001012856.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003647	XP_562511.3
Homo sapiens (human)	EMC1	NP_055862.1
Pan troglodytes (chimpanzee)	EMC1	XP_001162981.2
Rattus norvegicus (Norway rat)	Emc1	NP_001102160.1
Xenopus tropicalis (tropical clawed frog)	emc1	XP_002943252.2

Gene Ontology
Bibliography

Related articles in PubMed

EMC3 coordinates surfactant protein and lipid homeostasis required for respiration.
Tang X, Snowball JM, Xu Y, Na CL, Weaver TE, Clair G, Kyle JE, Zink EM, Ansong C, Wei W, Huang M, Lin X, Whitsett JA
The Journal of clinical investigation127(12)4314-4325(2017 12)

ZMPSTE24 defends against influenza and other pathogenic viruses.
Fu B, Wang L, Li S, Dorf ME
The Journal of experimental medicine214(4)919-929(2017 04)

Contribution of calumin to embryogenesis through participation in the endoplasmic reticulum-associated degradation activity.
Yamamoto S, Yamazaki T, Komazaki S, Yamashita T, Osaki M, Matsubayashi M, Kidoya H, Takakura N, Yamazaki D, Kakizawa S
Developmental biology393(1)33-43(2014 Sep)

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
Nakano Y, Jahan I, Bonde G, Sun X, Hildebrand MS, Engelhardt JF, Smith RJ, Cornell RA, Fritzsch B, Bánfi B
PLoS genetics8(10)e1002966(2012)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Emc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Emc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00538	NM_001039200.2 Latest version!	Mus musculus ER membrane protein complex subunit 1 (Emc1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu12270	NM_146157.4 Latest version!	Mus musculus ER membrane protein complex subunit 1 (Emc1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Emc1 ( NM_001039200.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034289.1 Mus musculus ER membrane protein complex subunit 1 (Emc1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00538
Clone ID Related Accession (Same CDS sequence)	NM_001039200.2
Accession Version	NM_001039200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Mus musculus(house mouse)
Product	ER membrane protein complex subunit 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY281473.1, AK089289.1, AK049061.1 and AL807833.5. On Sep 25, 2013 this sequence version replaced NM_001039200.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCG CGGTGGCGTC TGGGTTCTGG ATCTGGGCTG CTGTGCTGCT CGTCCCTGCG 
GCCGCGGTCT ACGAAGACCA AGTGGGCAAG TTTGACTGGA GACAGCAGTA TGTTGGGAAG 
ATCAAGTTTG CCTCCTTGGA GTTTTCCCCT GGATCCAAGA AGTTGGTTGT GGCCACAGAG 
AAGAATGTGA TTGCGGCGTT AAATTCTCGG ACTGGAGAGA TCTTGTGGCG CCATGTTGAC 
AAAGGCACAG CAGAAGGAGC TGTGGATGCC ATGCTGGTCC ATGGACAAGA TGCAATCACT 
GTATCCAACG GAGGGCGCCT CATGCGTTCC TGGGAGACCA ACATCGGGGG CCTGAACTGG 
GAGATAACTC TGGACACTGG CAGTTTCCAG GCACTTGGGC TAGTGGGCCT TCAGGAGTCA 
GTGAGGTACA TTGCAGTTCT GAAGAAGACG ACCCTCACCC TCCACCACCT CTCCAGTGGG 
CACCTGAAGT GGGTGGAGCA TCTGCCAGAA AGTGACAGCA TCCTCTACCA GATGGTGTAT 
TCCTACGGCT CCGGGGTGGT GTGGGCCCTT GGCATCGTTC CCTTCAGCCA CGTGAACATC 
GTCAAGTTTA ACGTGGAGGA TGGAGAGATT GTTCAGCAGG TCAGGGTTTG GACTCCATGG 
CTGCAGCACC TCACTGGGGC CTGTGGTGTG GTAGATGAGG CTGTCCTGGT ATGCCCCGAC 
CCCAGCTCAC ACTCACTCCA CACTTTGGCC CTGGAGACGG AGTGGGAACT GCGACAGATC 
CCCCTGCAGT CTCCTGACTT AGAATTTGGA AGTGGATTCC AGCCCCAAGT GCTGCCCACA 
CAACCCAGCC CAGTGGCTCC TTCTCGAGCC CAGTTCTTCC TGCAGTTGTC CCCAAGCCAC 
TACGCTCTAC TGCACTACCA CCATGGTGCC GTGACTCTGC TTAAGAACTT CCCCCAGGCC 
ACGCTCGTCA GCTTCGCCAC CACAGGGGAG AAGACAGTGG CTGCAGTCAT GACCTGTCGC 
ACTGAAGTGC AAAAACCTGT CAGTGCTGGA GATGGGTCTG TGGCAAGCTT TCCTGAGACA 
TCTGGTGCAC AGGACTCTCT GGCTTGCTTC AACCAGACCT ACACCATTAA CCTGTACTTA 
GTGGAGACAG GTCGGCGGCT CCTAGACACT TCCATTTCCT TCAGCCTGGA GCAGAAGGGC 
ACGCGACCTG AGCAGCTGTA CATCCAGGTG TTCTTGAAGA AGGACGACTC GGTGGGCTAC 
CGGGCCTTGG TGCAGACACA GGACCATCTG CAGCTGTTCC TGCAGCAGCT TGCTGGGAAG 
GTGGTGTTGT GGAGCCGAGA GGAGTCCCTG GCAGAAGTCG TGTGCCTGGA GATGGTGGAT 
CTCCCCCTGA CTGGAGCGCA GGCTGAGTTG GAAGGAGAAT TTGGCAAGAA GGCAGATGGC 
TTGCTGGGGA TGTTCCTGAA ACGCCTGTCG TCCCAGCTCA TCCTGCTCCA GGCCTGGACT 
TCCCACCTCT GGAAAATGTT CTACGATGCT CGGAAGCCCC GAAGCCAGAT TAAGAATGAG 
ATCAACATCG ACACACTGGC TAGAGATGAG TTCAACCTGC AGAAAATGAT GGTGACGGTA 
ACAGCCTCAG GCAAGCTCTT TGGCATCGAG AGCAGCTCTG GAACCATTCT GTGGAAGCAG 
TATCTGCCCA ATGTTAAGCC AGACTCCTCC TTTAAGCTGA TGGTGCAGAG GACTACTGCC 
CATTTCCCCC ATCCACCCCA GTGCACGCTG CTGGTGAAGG ACAAGGAGAC AGGAATGAGC 
TCTCTGTTTG TCTTCAACCC CATTTTTGGC AAGTGGAGCC AGGTAGCTCC CCCAGTGCTG 
AAGCGCCCCA TCTTGCAGTC ACTGCTTCTC CCAGTCATGG ATCAAGACTA TGCCAAGGTG 
TTGCTGTTGG TGGATGACGA ATACAAGGTC ACAGCTTTCC CAGCTACGCG GAATGTCTTG 
CGGCAGCTGC ATGAACTTGC CCCCTCCATC TTCTTCTACT TGGTGGATGC AGAGCAGGGG 
CGGCTCAGTG GGTATCAGCT TCGAAAGGAT CTCACTACAG AACTGAGTTG GGAGCTGACC 
ATCCCCCCAG AAGTGCAGCG CGTCGTCAAG GTCAAGGGGA AGCGCAGCAG CGAGCACGTA 
CACTCCCAGG GCCGGGTGAT GGGGGATCGA AGTGTGCTCT ACAAGAGTCT GAACCCCAAC 
TTGCTGGCCG TGGTGACAGA GAGCACGGAT GTCCACCACG AGCGCACCTT TATTGGCATC 
TTCCTCATTG ATGGTGTCAC TGGCCGCATC ATCCACTCCT CCGTGCAGAA GAAGGCCAGG 
GGCCCTGTCC ACCTTGTGCA TTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATTCTAAG 
GCTCGGCGCA ATGAGCTGAC GGCCCTGGAG CTCTACGAGG GCACTGAACA GTACAATGCC 
ACTGCCTTCA GCTCTCTAGA CCGACCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTACATC 
TTCCCCTCCT CCATCAGTGC CATGGAAGCT ACCATCACCG AGAGGGGCAT CACCAGCCGG 
CACTTGCTCA TTGGGCTGCC TTCTGGAGCA ATCCTTTCCC TCCCCAAGGC CTTGCTGGAT 
CCCCGGCGCC CAGAGATCCC AACAGAGCAA AGCAGAGAAG AGAACCTGAT CCCATATTCT 
CCAGATGTTC AGGTCCATGC AGAGCGATTC ATCAACTATA ACCAGACAGT CTCTCGAATG 
CGAGGCATCT ATACAGCGCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTACGGT 
TTGGATATTT ACCAAACTCG AGTTTACCCG TCCAAGCAGT TTGATGTCCT GAAAGATGAC 
TACGACTATG TGCTCATCAG CAGTGTCCTT TTTGGCCTGG TTTTTGCCAC CATGATCACA 
AAGAGGCTGG CACAGGTGAA ACTCCTCAAT CGAGCCTGGC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034289.1
CDS	34..3018
Translation	MAVAVASGFWIWAAVLLVPAAAVYEDQVGKFDWRQQYVGKIKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLVHGQDAITVSNGGRLMRSWETNIGGLNWEITLDTGSFQALGLVGLQESVRYIAVLKKTTLTLHHLSSGHLKWVEHLPESDSILYQMVYSYGSGVVWALGIVPFSHVNIVKFNVEDGEIVQQVRVWTPWLQHLTGACGVVDEAVLVCPDPSSHSLHTLALETEWELRQIPLQSPDLEFGSGFQPQVLPTQPSPVAPSRAQFFLQLSPSHYALLHYHHGAVTLLKNFPQATLVSFATTGEKTVAAVMTCRTEVQKPVSAGDGSVASFPETSGAQDSLACFNQTYTINLYLVETGRRLLDTSISFSLEQKGTRPEQLYIQVFLKKDDSVGYRALVQTQDHLQLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVTVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKETGMSSLFVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLVDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLSGYQLRKDLTTELSWELTIPPEVQRVVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGIFLIDGVTGRIIHSSVQKKARGPVHLVHSENWVVYQYWNSKARRNELTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQVHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001039200.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ER membrane protein complex subunit 1 (Emc1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039200.2

ORF Insert Sequence:

ATGGCGGTCG CGGTGGCGTC TGGGTTCTGG ATCTGGGCTG CTGTGCTGCT CGTCCCTGCG 
GCCGCGGTCT ACGAAGACCA AGTGGGCAAG TTTGACTGGA GACAGCAGTA TGTTGGGAAG 
ATCAAGTTTG CCTCCTTGGA GTTTTCCCCT GGATCCAAGA AGTTGGTTGT GGCCACAGAG 
AAGAATGTGA TTGCGGCGTT AAATTCTCGG ACTGGAGAGA TCTTGTGGCG CCATGTTGAC 
AAAGGCACAG CAGAAGGAGC TGTGGATGCC ATGCTGGTCC ATGGACAAGA TGCAATCACT 
GTATCCAACG GAGGGCGCCT CATGCGTTCC TGGGAGACCA ACATCGGGGG CCTGAACTGG 
GAGATAACTC TGGACACTGG CAGTTTCCAG GCACTTGGGC TAGTGGGCCT TCAGGAGTCA 
GTGAGGTACA TTGCAGTTCT GAAGAAGACG ACCCTCACCC TCCACCACCT CTCCAGTGGG 
CACCTGAAGT GGGTGGAGCA TCTGCCAGAA AGTGACAGCA TCCTCTACCA GATGGTGTAT 
TCCTACGGCT CCGGGGTGGT GTGGGCCCTT GGCATCGTTC CCTTCAGCCA CGTGAACATC 
GTCAAGTTTA ACGTGGAGGA TGGAGAGATT GTTCAGCAGG TCAGGGTTTG GACTCCATGG 
CTGCAGCACC TCACTGGGGC CTGTGGTGTG GTAGATGAGG CTGTCCTGGT ATGCCCCGAC 
CCCAGCTCAC ACTCACTCCA CACTTTGGCC CTGGAGACGG AGTGGGAACT GCGACAGATC 
CCCCTGCAGT CTCCTGACTT AGAATTTGGA AGTGGATTCC AGCCCCAAGT GCTGCCCACA 
CAACCCAGCC CAGTGGCTCC TTCTCGAGCC CAGTTCTTCC TGCAGTTGTC CCCAAGCCAC 
TACGCTCTAC TGCACTACCA CCATGGTGCC GTGACTCTGC TTAAGAACTT CCCCCAGGCC 
ACGCTCGTCA GCTTCGCCAC CACAGGGGAG AAGACAGTGG CTGCAGTCAT GACCTGTCGC 
ACTGAAGTGC AAAAACCTGT CAGTGCTGGA GATGGGTCTG TGGCAAGCTT TCCTGAGACA 
TCTGGTGCAC AGGACTCTCT GGCTTGCTTC AACCAGACCT ACACCATTAA CCTGTACTTA 
GTGGAGACAG GTCGGCGGCT CCTAGACACT TCCATTTCCT TCAGCCTGGA GCAGAAGGGC 
ACGCGACCTG AGCAGCTGTA CATCCAGGTG TTCTTGAAGA AGGACGACTC GGTGGGCTAC 
CGGGCCTTGG TGCAGACACA GGACCATCTG CAGCTGTTCC TGCAGCAGCT TGCTGGGAAG 
GTGGTGTTGT GGAGCCGAGA GGAGTCCCTG GCAGAAGTCG TGTGCCTGGA GATGGTGGAT 
CTCCCCCTGA CTGGAGCGCA GGCTGAGTTG GAAGGAGAAT TTGGCAAGAA GGCAGATGGC 
TTGCTGGGGA TGTTCCTGAA ACGCCTGTCG TCCCAGCTCA TCCTGCTCCA GGCCTGGACT 
TCCCACCTCT GGAAAATGTT CTACGATGCT CGGAAGCCCC GAAGCCAGAT TAAGAATGAG 
ATCAACATCG ACACACTGGC TAGAGATGAG TTCAACCTGC AGAAAATGAT GGTGACGGTA 
ACAGCCTCAG GCAAGCTCTT TGGCATCGAG AGCAGCTCTG GAACCATTCT GTGGAAGCAG 
TATCTGCCCA ATGTTAAGCC AGACTCCTCC TTTAAGCTGA TGGTGCAGAG GACTACTGCC 
CATTTCCCCC ATCCACCCCA GTGCACGCTG CTGGTGAAGG ACAAGGAGAC AGGAATGAGC 
TCTCTGTTTG TCTTCAACCC CATTTTTGGC AAGTGGAGCC AGGTAGCTCC CCCAGTGCTG 
AAGCGCCCCA TCTTGCAGTC ACTGCTTCTC CCAGTCATGG ATCAAGACTA TGCCAAGGTG 
TTGCTGTTGG TGGATGACGA ATACAAGGTC ACAGCTTTCC CAGCTACGCG GAATGTCTTG 
CGGCAGCTGC ATGAACTTGC CCCCTCCATC TTCTTCTACT TGGTGGATGC AGAGCAGGGG 
CGGCTCAGTG GGTATCAGCT TCGAAAGGAT CTCACTACAG AACTGAGTTG GGAGCTGACC 
ATCCCCCCAG AAGTGCAGCG CGTCGTCAAG GTCAAGGGGA AGCGCAGCAG CGAGCACGTA 
CACTCCCAGG GCCGGGTGAT GGGGGATCGA AGTGTGCTCT ACAAGAGTCT GAACCCCAAC 
TTGCTGGCCG TGGTGACAGA GAGCACGGAT GTCCACCACG AGCGCACCTT TATTGGCATC 
TTCCTCATTG ATGGTGTCAC TGGCCGCATC ATCCACTCCT CCGTGCAGAA GAAGGCCAGG 
GGCCCTGTCC ACCTTGTGCA TTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATTCTAAG 
GCTCGGCGCA ATGAGCTGAC GGCCCTGGAG CTCTACGAGG GCACTGAACA GTACAATGCC 
ACTGCCTTCA GCTCTCTAGA CCGACCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTACATC 
TTCCCCTCCT CCATCAGTGC CATGGAAGCT ACCATCACCG AGAGGGGCAT CACCAGCCGG 
CACTTGCTCA TTGGGCTGCC TTCTGGAGCA ATCCTTTCCC TCCCCAAGGC CTTGCTGGAT 
CCCCGGCGCC CAGAGATCCC AACAGAGCAA AGCAGAGAAG AGAACCTGAT CCCATATTCT 
CCAGATGTTC AGGTCCATGC AGAGCGATTC ATCAACTATA ACCAGACAGT CTCTCGAATG 
CGAGGCATCT ATACAGCGCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTACGGT 
TTGGATATTT ACCAAACTCG AGTTTACCCG TCCAAGCAGT TTGATGTCCT GAAAGATGAC 
TACGACTATG TGCTCATCAG CAGTGTCCTT TTTGGCCTGG TTTTTGCCAC CATGATCACA 
AAGAGGCTGG CACAGGTGAA ACTCCTCAAT CGAGCCTGGC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emc1 ( NM_146157.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666269.2 Mus musculus ER membrane protein complex subunit 1 (Emc1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12270
Clone ID Related Accession (Same CDS sequence)	NM_146157.4
Accession Version	NM_146157.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Mus musculus(house mouse)
Product	ER membrane protein complex subunit 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY281473.1, AK049061.1 and AL807833.5. On Sep 25, 2013 this sequence version replaced NM_146157.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCG CGGTGGCGTC TGGGTTCTGG ATCTGGGCTG CTGTGCTGCT CGTCCCTGCG 
GCCGCGGTCT ACGAAGACCA AGTGGGCAAG TTTGACTGGA GACAGCAGTA TGTTGGGAAG 
ATCAAGTTTG CCTCCTTGGA GTTTTCCCCT GGATCCAAGA AGTTGGTTGT GGCCACAGAG 
AAGAATGTGA TTGCGGCGTT AAATTCTCGG ACTGGAGAGA TCTTGTGGCG CCATGTTGAC 
AAAGGCACAG CAGAAGGAGC TGTGGATGCC ATGCTGGTCC ATGGACAAGA TGCAATCACT 
GTATCCAACG GAGGGCGCCT CATGCGTTCC TGGGAGACCA ACATCGGGGG CCTGAACTGG 
GAGATAACTC TGGACACTGG CAGTTTCCAG GCACTTGGGC TAGTGGGCCT TCAGGAGTCA 
GTGAGGTACA TTGCAGTTCT GAAGAAGACG ACCCTCACCC TCCACCACCT CTCCAGTGGG 
CACCTGAAGT GGGTGGAGCA TCTGCCAGAA AGTGACAGCA TCCTCTACCA GATGGTGTAT 
TCCTACGGCT CCGGGGTGGT GTGGGCCCTT GGCATCGTTC CCTTCAGCCA CGTGAACATC 
GTCAAGTTTA ACGTGGAGGA TGGAGAGATT GTTCAGCAGG TCAGGGTTTG GACTCCATGG 
CTGCAGCACC TCACTGGGGC CTGTGGTGTG GTAGATGAGG CTGTCCTGGT ATGCCCCGAC 
CCCAGCTCAC ACTCACTCCA CACTTTGGCC CTGGAGACGG AGTGGGAACT GCGACAGATC 
CCCCTGCAGT CTCCTGACTT AGAATTTGGA AGTGGATTCC AGCCCCAAGT GCTGCCCACA 
CAACCCAGCC CAGTGGCTCC TTCTCGAGCC CAGTTCTTCC TGCAGTTGTC CCCAAGCCAC 
TACGCTCTAC TGCACTACCA CCATGGTGCC GTGACTCTGC TTAAGAACTT CCCCCAGGCC 
ACGCTCGTCA GCTTCGCCAC CACAGGGGAG AAGACAGTGG CTGCAGTCAT GACCTGTCGC 
ACTGAAGTGC AAAAACCTGT CAGTGCTGGA GATGGGTCTG TGGCAAGCTT TCCTGAGACA 
TCTGGTGCAC AGGACTCTCT GGCTTGCTTC AACCAGACCT ACACCATTAA CCTGTACTTA 
GTGGAGACAG GTCGGCGGCT CCTAGACACT TCCATTTCCT TCAGCCTGGA GCAGAAGGGC 
ACGCGACCTG AGCAGCTGTA CATCCAGGTG TTCTTGAAGA AGGACGACTC GGTGGGCTAC 
CGGGCCTTGG TGCAGACACA GGACCATCTG CAGCTGTTCC TGCAGCAGCT TGCTGGGAAG 
GTGGTGTTGT GGAGCCGAGA GGAGTCCCTG GCAGAAGTCG TGTGCCTGGA GATGGTGGAT 
CTCCCCCTGA CTGGAGCGCA GGCTGAGTTG GAAGGAGAAT TTGGCAAGAA GGCAGCGATT 
CAAGATGGCT TGCTGGGGAT GTTCCTGAAA CGCCTGTCGT CCCAGCTCAT CCTGCTCCAG 
GCCTGGACTT CCCACCTCTG GAAAATGTTC TACGATGCTC GGAAGCCCCG AAGCCAGATT 
AAGAATGAGA TCAACATCGA CACACTGGCT AGAGATGAGT TCAACCTGCA GAAAATGATG 
GTGACGGTAA CAGCCTCAGG CAAGCTCTTT GGCATCGAGA GCAGCTCTGG AACCATTCTG 
TGGAAGCAGT ATCTGCCCAA TGTTAAGCCA GACTCCTCCT TTAAGCTGAT GGTGCAGAGG 
ACTACTGCCC ATTTCCCCCA TCCACCCCAG TGCACGCTGC TGGTGAAGGA CAAGGAGACA 
GGAATGAGCT CTCTGTTTGT CTTCAACCCC ATTTTTGGCA AGTGGAGCCA GGTAGCTCCC 
CCAGTGCTGA AGCGCCCCAT CTTGCAGTCA CTGCTTCTCC CAGTCATGGA TCAAGACTAT 
GCCAAGGTGT TGCTGTTGGT GGATGACGAA TACAAGGTCA CAGCTTTCCC AGCTACGCGG 
AATGTCTTGC GGCAGCTGCA TGAACTTGCC CCCTCCATCT TCTTCTACTT GGTGGATGCA 
GAGCAGGGGC GGCTCAGTGG GTATCAGCTT CGAAAGGATC TCACTACAGA ACTGAGTTGG 
GAGCTGACCA TCCCCCCAGA AGTGCAGCGC GTCGTCAAGG TCAAGGGGAA GCGCAGCAGC 
GAGCACGTAC ACTCCCAGGG CCGGGTGATG GGGGATCGAA GTGTGCTCTA CAAGAGTCTG 
AACCCCAACT TGCTGGCCGT GGTGACAGAG AGCACGGATG TCCACCACGA GCGCACCTTT 
ATTGGCATCT TCCTCATTGA TGGTGTCACT GGCCGCATCA TCCACTCCTC CGTGCAGAAG 
AAGGCCAGGG GCCCTGTCCA CCTTGTGCAT TCAGAGAACT GGGTGGTGTA CCAGTACTGG 
AATTCTAAGG CTCGGCGCAA TGAGCTGACG GCCCTGGAGC TCTACGAGGG CACTGAACAG 
TACAATGCCA CTGCCTTCAG CTCTCTAGAC CGACCCCAGC TGCCCCAGGT CCTCCAGCAG 
TCCTACATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCTA CCATCACCGA GAGGGGCATC 
ACCAGCCGGC ACTTGCTCAT TGGGCTGCCT TCTGGAGCAA TCCTTTCCCT CCCCAAGGCC 
TTGCTGGATC CCCGGCGCCC AGAGATCCCA ACAGAGCAAA GCAGAGAAGA GAACCTGATC 
CCATATTCTC CAGATGTTCA GGTCCATGCA GAGCGATTCA TCAACTATAA CCAGACAGTC 
TCTCGAATGC GAGGCATCTA TACAGCGCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG 
GCCTACGGTT TGGATATTTA CCAAACTCGA GTTTACCCGT CCAAGCAGTT TGATGTCCTG 
AAAGATGACT ACGACTATGT GCTCATCAGC AGTGTCCTTT TTGGCCTGGT TTTTGCCACC 
ATGATCACAA AGAGGCTGGC ACAGGTGAAA CTCCTCAATC GAGCCTGGCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666269.2
CDS	34..3027
Translation	MAVAVASGFWIWAAVLLVPAAAVYEDQVGKFDWRQQYVGKIKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLVHGQDAITVSNGGRLMRSWETNIGGLNWEITLDTGSFQALGLVGLQESVRYIAVLKKTTLTLHHLSSGHLKWVEHLPESDSILYQMVYSYGSGVVWALGIVPFSHVNIVKFNVEDGEIVQQVRVWTPWLQHLTGACGVVDEAVLVCPDPSSHSLHTLALETEWELRQIPLQSPDLEFGSGFQPQVLPTQPSPVAPSRAQFFLQLSPSHYALLHYHHGAVTLLKNFPQATLVSFATTGEKTVAAVMTCRTEVQKPVSAGDGSVASFPETSGAQDSLACFNQTYTINLYLVETGRRLLDTSISFSLEQKGTRPEQLYIQVFLKKDDSVGYRALVQTQDHLQLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVTVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKETGMSSLFVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLVDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLSGYQLRKDLTTELSWELTIPPEVQRVVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGIFLIDGVTGRIIHSSVQKKARGPVHLVHSENWVVYQYWNSKARRNELTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQVHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_146157.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ER membrane protein complex subunit 1 (Emc1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146157.4

ORF Insert Sequence:

ATGGCGGTCG CGGTGGCGTC TGGGTTCTGG ATCTGGGCTG CTGTGCTGCT CGTCCCTGCG 
GCCGCGGTCT ACGAAGACCA AGTGGGCAAG TTTGACTGGA GACAGCAGTA TGTTGGGAAG 
ATCAAGTTTG CCTCCTTGGA GTTTTCCCCT GGATCCAAGA AGTTGGTTGT GGCCACAGAG 
AAGAATGTGA TTGCGGCGTT AAATTCTCGG ACTGGAGAGA TCTTGTGGCG CCATGTTGAC 
AAAGGCACAG CAGAAGGAGC TGTGGATGCC ATGCTGGTCC ATGGACAAGA TGCAATCACT 
GTATCCAACG GAGGGCGCCT CATGCGTTCC TGGGAGACCA ACATCGGGGG CCTGAACTGG 
GAGATAACTC TGGACACTGG CAGTTTCCAG GCACTTGGGC TAGTGGGCCT TCAGGAGTCA 
GTGAGGTACA TTGCAGTTCT GAAGAAGACG ACCCTCACCC TCCACCACCT CTCCAGTGGG 
CACCTGAAGT GGGTGGAGCA TCTGCCAGAA AGTGACAGCA TCCTCTACCA GATGGTGTAT 
TCCTACGGCT CCGGGGTGGT GTGGGCCCTT GGCATCGTTC CCTTCAGCCA CGTGAACATC 
GTCAAGTTTA ACGTGGAGGA TGGAGAGATT GTTCAGCAGG TCAGGGTTTG GACTCCATGG 
CTGCAGCACC TCACTGGGGC CTGTGGTGTG GTAGATGAGG CTGTCCTGGT ATGCCCCGAC 
CCCAGCTCAC ACTCACTCCA CACTTTGGCC CTGGAGACGG AGTGGGAACT GCGACAGATC 
CCCCTGCAGT CTCCTGACTT AGAATTTGGA AGTGGATTCC AGCCCCAAGT GCTGCCCACA 
CAACCCAGCC CAGTGGCTCC TTCTCGAGCC CAGTTCTTCC TGCAGTTGTC CCCAAGCCAC 
TACGCTCTAC TGCACTACCA CCATGGTGCC GTGACTCTGC TTAAGAACTT CCCCCAGGCC 
ACGCTCGTCA GCTTCGCCAC CACAGGGGAG AAGACAGTGG CTGCAGTCAT GACCTGTCGC 
ACTGAAGTGC AAAAACCTGT CAGTGCTGGA GATGGGTCTG TGGCAAGCTT TCCTGAGACA 
TCTGGTGCAC AGGACTCTCT GGCTTGCTTC AACCAGACCT ACACCATTAA CCTGTACTTA 
GTGGAGACAG GTCGGCGGCT CCTAGACACT TCCATTTCCT TCAGCCTGGA GCAGAAGGGC 
ACGCGACCTG AGCAGCTGTA CATCCAGGTG TTCTTGAAGA AGGACGACTC GGTGGGCTAC 
CGGGCCTTGG TGCAGACACA GGACCATCTG CAGCTGTTCC TGCAGCAGCT TGCTGGGAAG 
GTGGTGTTGT GGAGCCGAGA GGAGTCCCTG GCAGAAGTCG TGTGCCTGGA GATGGTGGAT 
CTCCCCCTGA CTGGAGCGCA GGCTGAGTTG GAAGGAGAAT TTGGCAAGAA GGCAGCGATT 
CAAGATGGCT TGCTGGGGAT GTTCCTGAAA CGCCTGTCGT CCCAGCTCAT CCTGCTCCAG 
GCCTGGACTT CCCACCTCTG GAAAATGTTC TACGATGCTC GGAAGCCCCG AAGCCAGATT 
AAGAATGAGA TCAACATCGA CACACTGGCT AGAGATGAGT TCAACCTGCA GAAAATGATG 
GTGACGGTAA CAGCCTCAGG CAAGCTCTTT GGCATCGAGA GCAGCTCTGG AACCATTCTG 
TGGAAGCAGT ATCTGCCCAA TGTTAAGCCA GACTCCTCCT TTAAGCTGAT GGTGCAGAGG 
ACTACTGCCC ATTTCCCCCA TCCACCCCAG TGCACGCTGC TGGTGAAGGA CAAGGAGACA 
GGAATGAGCT CTCTGTTTGT CTTCAACCCC ATTTTTGGCA AGTGGAGCCA GGTAGCTCCC 
CCAGTGCTGA AGCGCCCCAT CTTGCAGTCA CTGCTTCTCC CAGTCATGGA TCAAGACTAT 
GCCAAGGTGT TGCTGTTGGT GGATGACGAA TACAAGGTCA CAGCTTTCCC AGCTACGCGG 
AATGTCTTGC GGCAGCTGCA TGAACTTGCC CCCTCCATCT TCTTCTACTT GGTGGATGCA 
GAGCAGGGGC GGCTCAGTGG GTATCAGCTT CGAAAGGATC TCACTACAGA ACTGAGTTGG 
GAGCTGACCA TCCCCCCAGA AGTGCAGCGC GTCGTCAAGG TCAAGGGGAA GCGCAGCAGC 
GAGCACGTAC ACTCCCAGGG CCGGGTGATG GGGGATCGAA GTGTGCTCTA CAAGAGTCTG 
AACCCCAACT TGCTGGCCGT GGTGACAGAG AGCACGGATG TCCACCACGA GCGCACCTTT 
ATTGGCATCT TCCTCATTGA TGGTGTCACT GGCCGCATCA TCCACTCCTC CGTGCAGAAG 
AAGGCCAGGG GCCCTGTCCA CCTTGTGCAT TCAGAGAACT GGGTGGTGTA CCAGTACTGG 
AATTCTAAGG CTCGGCGCAA TGAGCTGACG GCCCTGGAGC TCTACGAGGG CACTGAACAG 
TACAATGCCA CTGCCTTCAG CTCTCTAGAC CGACCCCAGC TGCCCCAGGT CCTCCAGCAG 
TCCTACATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCTA CCATCACCGA GAGGGGCATC 
ACCAGCCGGC ACTTGCTCAT TGGGCTGCCT TCTGGAGCAA TCCTTTCCCT CCCCAAGGCC 
TTGCTGGATC CCCGGCGCCC AGAGATCCCA ACAGAGCAAA GCAGAGAAGA GAACCTGATC 
CCATATTCTC CAGATGTTCA GGTCCATGCA GAGCGATTCA TCAACTATAA CCAGACAGTC 
TCTCGAATGC GAGGCATCTA TACAGCGCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG 
GCCTACGGTT TGGATATTTA CCAAACTCGA GTTTACCCGT CCAAGCAGTT TGATGTCCTG 
AAAGATGACT ACGACTATGT GCTCATCAGC AGTGTCCTTT TTGGCCTGGT TTTTGCCACC 
ATGATCACAA AGAGGCTGGC ACAGGTGAAA CTCCTCAATC GAGCCTGGCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ZMPSTE24 defends against influenza and other pathogenic viruses.
The Journal of experimental medicine214(4)919-929(2017 04)
Fu B,Wang L,Li S,Dorf ME

Contribution of calumin to embryogenesis through participation in the endoplasmic reticulum-associated degradation activity.
Developmental biology393(1)33-43(2014 Sep)
Yamamoto S,Yamazaki T,Komazaki S,Yamashita T,Osaki M,Matsubayashi M,Kidoya H,Takakura N,Yamazaki D,Kakizawa S

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
PLoS genetics8(10)e1002966(2012)
Nakano Y,Jahan I,Bonde G,Sun X,Hildebrand MS,Engelhardt JF,Smith RJ,Cornell RA,Fritzsch B,Bánfi B

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

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Emc1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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