Dennd4b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dennd4b
Entrez Gene ID	361987
Full Name	DENN domain containing 4B
Synonyms	RGD1560320
General protein information	Preferred Names DENN domain containing 4B Names DENN domain-containing protein 4B LOW QUALITY PROTEIN: DENN domain-containing protein 4B DENN/MADD domain containing 4B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_008761250.2	XP_008759472.1	DENN domain-containing protein 4B isoform X4
XM_008761249.2	XP_008759471.1	DENN domain-containing protein 4B isoform X3
XM_008775188.2	XP_008773410.1	LOW QUALITY PROTEIN: DENN domain-containing protein 4B isoform X5
XM_008761248.2	XP_008759470.1	DENN domain-containing protein 4B isoform X1
XM_342287.9	XP_342288.6	DENN domain-containing protein 4B isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DENND4B	XP_002686064.2
Danio rerio (zebrafish)	LOC798528	XP_002665116.3
Homo sapiens (human)	DENND4B	NP_055671.2
Pan troglodytes (chimpanzee)	DENND4B	XP_513830.3
Mus musculus (house mouse)	Dennd4b	NP_958809.3
Rattus norvegicus (Norway rat)	Dennd4b	XP_342288.5
Macaca mulatta (Rhesus monkey)	LOC718490	XP_002808284.1
Canis lupus familiaris (dog)	DENND4B	XP_537262.4

Gene Ontology

The following Dennd4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dennd4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa48090	XM_008775188.2 Latest version!	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa48096	XM_008761250.2 Latest version!	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa48093	XM_008761248.2 Latest version!	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa48094	XM_342287.9 Latest version!	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa48095	XM_008761249.2 Latest version!	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dennd4b ( XM_008775188.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008773410.1 Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa48090
Clone ID Related Accession (Same CDS sequence)	XM_008775188.2
Accession Version	XM_008775188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: DENN domain-containing protein 4B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000070.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGGCAG ATGCAGTGAG TGAAGGGGGG GCCATGGCAG AGGAGCGGCC TCCCCGGTTG 
GTGGATTACT TCGTGGTGGC TGGGCTTGCA GGAAATGGAG CACCTATACC TGAGGAGACG 
TGGGTTCCTG AGCCTACTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAACC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAA GAGGTGCCCC AGGGCTATAC ATGCATTCAG 
ACTTCTGCTG GTGGTCACCC CTTGGAGCTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGCAGGGG GCGTGACAAG CCCCCCCTTG TTGAGCTGGG GGTATTATAT 
GACGGGAAAG AACGACCTAA GCCTGGGTTC CAAGTGCTTG ACACGACGCC ATATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAT GTACCGGCGT 
GCAGCAGAAG GGGCAGGTCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTTCTGCCC 
AGCAAGGGTG AGAGCACTCC TCATACTTAC TGCCGGTTAC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTT CCTGTGCTAC AAGGTGGGCC TGGCGAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT AGGCCGCTAC CCAGAGGAAG ACAATGAGGC GTTTCCGCTG 
CCTGAGTCAG TGCCAGTCTT CTGCCTGCCC ATGGGAGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCAGTACC CGTCTTTTCC ACCTTTGTGC TCACGGGAGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCGTT GCAGTTCTAT GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTCCTGAGT GCCGTGGAAC GGGGACGGGC CCTGGGAGGC 
CGAGCAGTGC GCAGTCGGAG AGCCATTGCT GTGCTGTCCC GCTGGCCCGC CTTCCCGGCC 
TTCCGAGCCT TCCTCACCTT CCTTTACCGC TACTCCGTCT CAGGCCCCCA TCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCGT TCCCTTCCCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGTCAACC TGTCTCCTCA 
CCCCTGCCCC TGAGTGGTGC TAGCTTTTTA CAGCTGCTGC AGAACCTGGG CCCAGAATTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCTCTGCGG 
CCAGATCTGC TTACCAGTGT CTGCGAGGCC CTTGTCTCAA TGATTTTCCC TCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTA GTGCTGGCGG ATGTGCTGAG TGCCCCTGTG 
CCCTTCATTG TGGGTATCCA CTCCAGTTAT TTCGATCTGC ATGACCCACC TGCTGACGTC 
ATCTGTGTTG ATCTTGACAC CAACACGCTC TTTCAAACGG AGGAAAAGAA GCCCCTCTCT 
GCTAGGACCC TGCCCCGAAG ACCTTACAAG CTTCTGCTGG CCACACTGAC GAATCTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTATG AGGCAGTGTG TGGCCGCAGG ACTCGCCTAG AACGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGA ACTTCCTTCG ACCTCTCACC 
CAGGCCCCCT CTGAGGGGTC TCGAGATGTT GAGAACCTTT TCTACCTTCA GGGTTTCCTT 
AAGTCCCGGG AACGCTCCAG TCACAAGCTG TACTCTCAGC TCCTACACAC GCAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTCGGC TCTACCCGGC ATGCAGCCCT GGAATTCTTT 
GACTCTTGTG TTGACAAGGT TCACCCAGAG CAGGAGAAGC CTGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AACTATCGGG AAGTGAGCTC ACTGTCTTTA TCACACCCCC AGAGGAACCG 
CCAGTACTGG AAGGCAGTGA ATCTACTCCT CAGTACTGTT ATGATGGGTT TCCAGAGCTG 
AAGGCTGAGC TATTTGAGTC TCCTCAGGAA CAACAGGGGG CACTCCCTGT GCCAGGCCCA 
TCCCGTAGTG CCCCCAGCAG TCCTGCACCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGCGTGG CACAGAAGTC AGCCACTGTA CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT 
CACTGCTATG GACTGTGGTT CTTATGCCTA CCTGCCTATG TGCGTTCTGC ACCTTCCCGG 
GTGCGAGCAT TACACACAGC CTACCATGTA TTACGTGAGA TGGAGAGCCG CAAGGTGGTG 
CTCCCTGATG AGGTCTGTTA CCGGGTACTG ATGCAGCTCT GCTCAAACTA TGGGCAACCC 
GTGTTGTCTG TGCGAGTCAT GCTGGAGATG CGACGGGCAG GCATTGTGCC CAACACCATC 
ACCTATGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAAT GGCCATCTGG TACTCCGGGT 
GGACGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCTGGG GGGCGGCTCA GTTCCGCCAG 
CCCTTAAAGG ACCGAAGGCG GCTGCAGCAG GAGCAGCAGG AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGGTGGC CGAGCAGCAA AAGTCGGGCA GCTCTCAGAC AGAGCCCTGT 
CTGGAGCGTC CTTCCCCAAC CCGCCCTCTT CAGCGCCAGA CTACTTGGGC TGGACGCAGT 
CTACGGGAGC CATCTTCACC CATCGGGCGC CTGGTCAAGA GCGGCAGCCT ANGCAGCGCC 
CGCGGGGCAC AGCCCACTGT GGAGGCTGGC GTGGCTCACA GTGAGTACCC TTATCTGACC 
TCCTCCCTCT TCTCACTGGG CTTCAGTGAC GAGGGAGGAC TAGAAAAAGG GCTGGGTGTC 
CGCCTCCCAA ACCAGCTGCT TACCCCTTCC CGCCGCTCCT CTGCTTCCCG GATCTCCGTA 
CCTGAGTTGC CCCCTGACCT GCCTCCTGCG GCTCGCCGGA GTCCCATGGA TAGCCTTCTG 
TGGCCCCGAG AACGCCCTGG ATCCACTGCT TCTGAGAGTT CTGCTTCTCT GGGCAGTGAG 
TGGGATATCT CAGAGTCTTC TCTCAGCAGC CTGAGTCTTC GACGTTCCTC AGAGCGTCTC 
AGCGACACCC CTGGAGCTTT CCAACCACCC TCCCTAGAAA TTCTGATGTC TAGCTGCTCC 
CTGTGCCATG CCTGTGATTC CCTGGTGTAT GATGAGGAAA TCATGGCCGG CTGGGCACCG 
GATGACTCTA ACCTCAATAC GACTTGTCCC TTCTGCGCCT GTCCCTTCGT GCCCCTGCTT 
AGTGTCCAGA CTCTTGATTC TCGACCCAGT GCGCCTAGTC CAAAGTCTGC CCTTGCTGGT 
GCCGGTGGCT GCAAAGATGC TCCTGTCCCT GGGGGTCCTG GCCCTGTGCT CAGTGACCGG 
AGGCTGTGCC TTGCCCTGGA TGAGCCCCAG CTCTGCAATG GGCATATGGG GAGTGCTTCC 
CGGCGAGTTG AGAATGGGGC ATGGGCGTAC CTCAGCCCTC TGGTGCTGCG TAAAGAACTA 
GAGTCCCTGG TAGAGAATGA GGGCATCGAG GTGTTGGCGT TGCCTGAACT GCCTGCTGCC 
CACCCTATCA TCTTCTGGAA CCTCCTGTGG TATTTCCAAC GGCTACGCCT GCCTAGTATT 
CTGCCAGGCC TGGTGTTGGC CTCCTGTAAT GGGCCGCCAC CCAGCCAGCT CTCCCAGGGA 
CCATCTTCAT GGCTCACCCC TGATCCAGCT TCTGTGCATG TGCGTCTGCT GTGGGACGTC 
CTGATCCCTG ACCCCAATAG CTGCCCGCCT CTCTACGTGC TCTGGAGGGT CCACAGCCAG 
ATCCCACAGC GGGTGGTGTG GCCAGGTCCA GTACCCTCCT GTCTTAGCTT GGCATTACTG 
GAGTCAGTGC TGCGCCATTT CGGACTCAAC GAGGTTCATA AAGCTGTTGG ACTTCTGTTG 
GAGACTCTAG GGCCCCCTCC CACTGGCCTG CACCTGCAGA GGGGAATCTA CCGTGAGATC 
TTATTCCTGA CGATGGCTGC TCTGGGCAAG GACCACGTGG ATATAGTGGC CTTCGACAAG 
AAGTACAAGT CTGCCTTTAA CAAGCTGGCC AGCAGCATGG GCAAGGAAGA GCTGAGGCAG 
CGGCGGGCAC AGATGCCTTC CCCCAAAGCC ATCGACTGCC GAAAGTGTTT TGGAGCACCT 
CTGGAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008773410.1
CDS	1052..5443
Translation	MEADAVSEGGAMAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYDGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGESTPHTYCRLPRNLNPGMWGPAVFLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVENLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSTRHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRVAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSNYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVWGAAQFRQPLKDRRRLQQEQQEQQQQQQQQQQQVAEQQKSGSSQTEPCLERPSPTRPLQRQTTWAGRSLREPSSPIGRLVKSGSLnullSARGAQPTVEAGVAHSEYPYLTSSLFSLGFSDEGGLEKGLGVRLPNQLLTPSRRSSASRISVPELPPDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKSALAGAGGCKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGIEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASCNGPPPSQLSQGPSSWLTPDPASVHVRLLWDVLIPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHFGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPSPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_008775188.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008775188.2

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAAGGGGGG GCCATGGCAG AGGAGCGGCC TCCCCGGTTG 
GTGGATTACT TCGTGGTGGC TGGGCTTGCA GGAAATGGAG CACCTATACC TGAGGAGACG 
TGGGTTCCTG AGCCTACTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAACC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAA GAGGTGCCCC AGGGCTATAC ATGCATTCAG 
ACTTCTGCTG GTGGTCACCC CTTGGAGCTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGCAGGGG GCGTGACAAG CCCCCCCTTG TTGAGCTGGG GGTATTATAT 
GACGGGAAAG AACGACCTAA GCCTGGGTTC CAAGTGCTTG ACACGACGCC ATATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAT GTACCGGCGT 
GCAGCAGAAG GGGCAGGTCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTTCTGCCC 
AGCAAGGGTG AGAGCACTCC TCATACTTAC TGCCGGTTAC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTT CCTGTGCTAC AAGGTGGGCC TGGCGAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT AGGCCGCTAC CCAGAGGAAG ACAATGAGGC GTTTCCGCTG 
CCTGAGTCAG TGCCAGTCTT CTGCCTGCCC ATGGGAGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCAGTACC CGTCTTTTCC ACCTTTGTGC TCACGGGAGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCGTT GCAGTTCTAT GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTCCTGAGT GCCGTGGAAC GGGGACGGGC CCTGGGAGGC 
CGAGCAGTGC GCAGTCGGAG AGCCATTGCT GTGCTGTCCC GCTGGCCCGC CTTCCCGGCC 
TTCCGAGCCT TCCTCACCTT CCTTTACCGC TACTCCGTCT CAGGCCCCCA TCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCGT TCCCTTCCCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGTCAACC TGTCTCCTCA 
CCCCTGCCCC TGAGTGGTGC TAGCTTTTTA CAGCTGCTGC AGAACCTGGG CCCAGAATTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCTCTGCGG 
CCAGATCTGC TTACCAGTGT CTGCGAGGCC CTTGTCTCAA TGATTTTCCC TCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTA GTGCTGGCGG ATGTGCTGAG TGCCCCTGTG 
CCCTTCATTG TGGGTATCCA CTCCAGTTAT TTCGATCTGC ATGACCCACC TGCTGACGTC 
ATCTGTGTTG ATCTTGACAC CAACACGCTC TTTCAAACGG AGGAAAAGAA GCCCCTCTCT 
GCTAGGACCC TGCCCCGAAG ACCTTACAAG CTTCTGCTGG CCACACTGAC GAATCTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTATG AGGCAGTGTG TGGCCGCAGG ACTCGCCTAG AACGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGA ACTTCCTTCG ACCTCTCACC 
CAGGCCCCCT CTGAGGGGTC TCGAGATGTT GAGAACCTTT TCTACCTTCA GGGTTTCCTT 
AAGTCCCGGG AACGCTCCAG TCACAAGCTG TACTCTCAGC TCCTACACAC GCAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTCGGC TCTACCCGGC ATGCAGCCCT GGAATTCTTT 
GACTCTTGTG TTGACAAGGT TCACCCAGAG CAGGAGAAGC CTGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AACTATCGGG AAGTGAGCTC ACTGTCTTTA TCACACCCCC AGAGGAACCG 
CCAGTACTGG AAGGCAGTGA ATCTACTCCT CAGTACTGTT ATGATGGGTT TCCAGAGCTG 
AAGGCTGAGC TATTTGAGTC TCCTCAGGAA CAACAGGGGG CACTCCCTGT GCCAGGCCCA 
TCCCGTAGTG CCCCCAGCAG TCCTGCACCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGCGTGG CACAGAAGTC AGCCACTGTA CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT 
CACTGCTATG GACTGTGGTT CTTATGCCTA CCTGCCTATG TGCGTTCTGC ACCTTCCCGG 
GTGCGAGCAT TACACACAGC CTACCATGTA TTACGTGAGA TGGAGAGCCG CAAGGTGGTG 
CTCCCTGATG AGGTCTGTTA CCGGGTACTG ATGCAGCTCT GCTCAAACTA TGGGCAACCC 
GTGTTGTCTG TGCGAGTCAT GCTGGAGATG CGACGGGCAG GCATTGTGCC CAACACCATC 
ACCTATGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAAT GGCCATCTGG TACTCCGGGT 
GGACGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCTGGG GGGCGGCTCA GTTCCGCCAG 
CCCTTAAAGG ACCGAAGGCG GCTGCAGCAG GAGCAGCAGG AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGGTGGC CGAGCAGCAA AAGTCGGGCA GCTCTCAGAC AGAGCCCTGT 
CTGGAGCGTC CTTCCCCAAC CCGCCCTCTT CAGCGCCAGA CTACTTGGGC TGGACGCAGT 
CTACGGGAGC CATCTTCACC CATCGGGCGC CTGGTCAAGA GCGGCAGCCT ANGCAGCGCC 
CGCGGGGCAC AGCCCACTGT GGAGGCTGGC GTGGCTCACA GTGAGTACCC TTATCTGACC 
TCCTCCCTCT TCTCACTGGG CTTCAGTGAC GAGGGAGGAC TAGAAAAAGG GCTGGGTGTC 
CGCCTCCCAA ACCAGCTGCT TACCCCTTCC CGCCGCTCCT CTGCTTCCCG GATCTCCGTA 
CCTGAGTTGC CCCCTGACCT GCCTCCTGCG GCTCGCCGGA GTCCCATGGA TAGCCTTCTG 
TGGCCCCGAG AACGCCCTGG ATCCACTGCT TCTGAGAGTT CTGCTTCTCT GGGCAGTGAG 
TGGGATATCT CAGAGTCTTC TCTCAGCAGC CTGAGTCTTC GACGTTCCTC AGAGCGTCTC 
AGCGACACCC CTGGAGCTTT CCAACCACCC TCCCTAGAAA TTCTGATGTC TAGCTGCTCC 
CTGTGCCATG CCTGTGATTC CCTGGTGTAT GATGAGGAAA TCATGGCCGG CTGGGCACCG 
GATGACTCTA ACCTCAATAC GACTTGTCCC TTCTGCGCCT GTCCCTTCGT GCCCCTGCTT 
AGTGTCCAGA CTCTTGATTC TCGACCCAGT GCGCCTAGTC CAAAGTCTGC CCTTGCTGGT 
GCCGGTGGCT GCAAAGATGC TCCTGTCCCT GGGGGTCCTG GCCCTGTGCT CAGTGACCGG 
AGGCTGTGCC TTGCCCTGGA TGAGCCCCAG CTCTGCAATG GGCATATGGG GAGTGCTTCC 
CGGCGAGTTG AGAATGGGGC ATGGGCGTAC CTCAGCCCTC TGGTGCTGCG TAAAGAACTA 
GAGTCCCTGG TAGAGAATGA GGGCATCGAG GTGTTGGCGT TGCCTGAACT GCCTGCTGCC 
CACCCTATCA TCTTCTGGAA CCTCCTGTGG TATTTCCAAC GGCTACGCCT GCCTAGTATT 
CTGCCAGGCC TGGTGTTGGC CTCCTGTAAT GGGCCGCCAC CCAGCCAGCT CTCCCAGGGA 
CCATCTTCAT GGCTCACCCC TGATCCAGCT TCTGTGCATG TGCGTCTGCT GTGGGACGTC 
CTGATCCCTG ACCCCAATAG CTGCCCGCCT CTCTACGTGC TCTGGAGGGT CCACAGCCAG 
ATCCCACAGC GGGTGGTGTG GCCAGGTCCA GTACCCTCCT GTCTTAGCTT GGCATTACTG 
GAGTCAGTGC TGCGCCATTT CGGACTCAAC GAGGTTCATA AAGCTGTTGG ACTTCTGTTG 
GAGACTCTAG GGCCCCCTCC CACTGGCCTG CACCTGCAGA GGGGAATCTA CCGTGAGATC 
TTATTCCTGA CGATGGCTGC TCTGGGCAAG GACCACGTGG ATATAGTGGC CTTCGACAAG 
AAGTACAAGT CTGCCTTTAA CAAGCTGGCC AGCAGCATGG GCAAGGAAGA GCTGAGGCAG 
CGGCGGGCAC AGATGCCTTC CCCCAAAGCC ATCGACTGCC GAAAGTGTTT TGGAGCACCT 
CTGGAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_008761250.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759472.1 Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa48096
Clone ID Related Accession (Same CDS sequence)	XM_008761250.2
Accession Version	XM_008761250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DENN domain-containing protein 4B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACC TATACCTGAG GAGACGTGGG TTCCTGAGCC TACTGGGCCC CTGCGCCCTC 
CCCGGCCAGC TGAACCTATC ACAGATGTGG CAGTCATCGC TAGGGCACTG GGCGAAGAGG 
TGCCCCAGGG CTATACATGC ATTCAGACTT CTGCTGGTGG TCACCCCTTG GAGCTCAGTG 
CTGGGCTCCT GGGTGGAACT CAACCAGTCA TCTGCTACCG CAGGGGGCGT GACAAGCCCC 
CCCTTGTTGA GCTGGGGGTA TTATATGACG GGAAAGAACG ACCTAAGCCT GGGTTCCAAG 
TGCTTGACAC GACGCCATAT AGCCACTCAG CCAACCTGGC CCCTCCAGGC CCTGGGCACC 
CCCGCACCTA CCTCATGTAC CGGCGTGCAG CAGAAGGGGC AGGTCTGCAT GCCCTGGGCA 
TCACTGACCT CTGCCTGGTT CTGCCCAGCA AGGTGGGGCC CAGCAGTGTT CCTGTGCTAC 
AAGGTGGGCC TGGCGAAGGC CAACACACTG GTGTATGAGG CAGAGCTGCT AGGCCGCTAC 
CCAGAGGAAG ACAATGAGGC GTTTCCGCTG CCTGAGTCAG TGCCAGTCTT CTGCCTGCCC 
ATGGGAGCCA CTATCGAGTG CTGGCCTGCC CAGACCAAGT ACCCAGTACC CGTCTTTTCC 
ACCTTTGTGC TCACGGGAGC TGCTGGTGAT AAGGTGTATG GTGCCGCGTT GCAGTTCTAT 
GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG AGGCAGGCAC GGGCACTGGG CCTCCTGAGT 
GCCGTGGAAC GGGGACGGGC CCTGGGAGGC CGAGCAGTGC GCAGTCGGAG AGCCATTGCT 
GTGCTGTCCC GCTGGCCCGC CTTCCCGGCC TTCCGAGCCT TCCTCACCTT CCTTTACCGC 
TACTCCGTCT CAGGCCCCCA TCGTCTGCCC CTGGAAGCGC ACATCTCCCA CTTCATTCAC 
AACGTCCCGT TCCCTTCCCC ACAGAGACCA CGCATCCTAG TGCAGATGTC TCCCTATGAC 
AACCTGCTCC TCTGTCAACC TGTCTCCTCA CCCCTGCCCC TGAGTGGTGC TAGCTTTTTA 
CAGCTGCTGC AGAACCTGGG CCCAGAATTG GCTATTACAT TGCTGCTGGC TGTGCTCACA 
GAGCACAAAC TGCTGGTCCA CTCTCTGCGG CCAGATCTGC TTACCAGTGT CTGCGAGGCC 
CTTGTCTCAA TGATTTTCCC TCTGCACTGG CAGTGCCCCT ACATTCCGCT GTGCCCGCTA 
GTGCTGGCGG ATGTGCTGAG TGCCCCTGTG CCCTTCATTG TGGGTATCCA CTCCAGTTAT 
TTCGATCTGC ATGACCCACC TGCTGACGTC ATCTGTGTTG ATCTTGACAC CAACACGCTC 
TTTCAAACGG AGGAAAAGAA GCCCCTCTCT GCTAGGACCC TGCCCCGAAG ACCTTACAAG 
CTTCTGCTGG CCACACTGAC GAATCTGTAC CAGCAGCTGG ACCAGACTTA CACTGGACCA 
GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG ACAGACTATG AGGCAGTGTG TGGCCGCAGG 
ACTCGCCTAG AACGGGAAGT CCAGGGAGCC TTCCTGCGCT TCATGGCCTG TCTGCTCAAA 
GGCTACCGGA ACTTCCTTCG ACCTCTCACC CAGGCCCCCT CTGAGGGGTC TCGAGATGTT 
GAGAACCTTT TCTACCTTCA GGGTTTCCTT AAGTCCCGGG AACGCTCCAG TCACAAGCTG 
TACTCTCAGC TCCTACACAC GCAGATGTTC TCCCAGTTCA TCGAGGAATG CTCTTTCGGC 
TCTACCCGGC ATGCAGCCCT GGAATTCTTT GACTCTTGTG TTGACAAGGT TCACCCAGAG 
CAGGAGAAGC CTGAGCCAAC ACCCTTAGTG GAGCTGGAGG AACTATCGGG AAGTGAGCTC 
ACTGTCTTTA TCACACCCCC AGAGGAACCG CCAGTACTGG AAGGCAGTGA ATCTACTCCT 
CAGTACTGTT ATGATGGGTT TCCAGAGCTG AAGGCTGAGC TATTTGAGTC TCCTCAGGAA 
CAACAGGGGG CACTCCCTGT GCCAGGCCCA TCCCGTAGTG CCCCCAGCAG TCCTGCACCT 
CGCCGTACCA AACAGGAGAT GAAGGTTGCA CAGCGCGTGG CACAGAAGTC AGCCACTGTA 
CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT CACTGCTATG GACTGTGGTT CTTATGCCTA 
CCTGCCTATG TGCGTTCTGC ACCTTCCCGG GTGCGAGCAT TACACACAGC CTACCATGTA 
TTACGTGAGA TGGAGAGCCG CAAGGTGGTG CTCCCTGATG AGGTCTGTTA CCGGGTACTG 
ATGCAGCTCT GCTCAAACTA TGGGCAACCC GTGTTGTCTG TGCGAGTCAT GCTGGAGATG 
CGACGGGCAG GCATTGTGCC CAACACCATC ACCTATGGCT ACTACAACAA GGCTGTGCTG 
GAAAGCAAAT GGCCATCTGG TACTCCGGGT GGACGCCTAC GCTGGGCCAA GCTCCGGAAT 
GTTGTCTGGG GGGCGGCTCA GTTCCGCCAG CCCTTAAAGG ACCGAAGGCG GCTGCAGCAG 
GAGCAGCAGG AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AACAGGTGGC CGAGCAGCAA 
AAGTCGGGCA GCTCTCAGAC AGAGCCCTGT CTGGAGCGTC CTTCCCCAAC CCGCCCTCTT 
CAGCGCCAGA CTACTTGGGC TGGACGCAGT CTACGGGAGC CATCTTCACC CATCGGGCGC 
CTGGTCAAGA GCGGCAGCCT AGGCAGCGCC CGCGGGGCAC AGCCCACTGT GGAGGCTGGC 
GTGGCTCACA TGATAGAGGC CTTGGGGGTC CTTGAACCCC GTGGATCACC TGTGCCTTGG 
CAGGATGGAA GTCTCTCAGA CCTGAGTCTG ACAGGGGAAG AACTGGCACC TGGAAGCAGC 
CCAGGGGGCT CAGGCTCAGC CCTGAGTGCC CAGTCCACTG AGGCCCTAGA AGGGATCAGT 
GGGCGGGGTT CCAAAACTAG TGGATGTCAG GAGGAGGCGG GCACCCCCAG AAAAGGGCTG 
GGTGCCCGCC TCCAACAGCT GCTTACCCCT TCCCGCCGCT CCTCTGCTTC CCGGATCTCC 
GTACCTGAGT TGCCCCCTGA CCTGCCTCCT GCGGCTCGCC GGAGTCCCAT GGATAGCCTT 
CTGTGGCCCC GAGAACGCCC TGGATCCACT GCTTCTGAGG TAAGTTCTGC TTCTCTGGGC 
AGTGAGTGGG ATATCTCAGA GTCTTCTCTC AGCAGCCTGA GTCTTCGACG TTCCTCAGAG 
CGTCTCAGCG ACACCCCTGG AGCTTTCCAA CCACCCTCCC TAGAAATTCT GATGTCTAGC 
TGCTCCCTGT GCCATGCCTG TGATTCCCTG GTGTATGATG AGGAAATCAT GGCCGGCTGG 
GCACCGGATG ACTCTAACCT CAATACGACT TGTCCCTTCT GCGCCTGTCC CTTCGTGCCC 
CTGCTTAGTG TCCAGACTCT TGATTCTCGA CCCAGTGCGC CTAGTCCAAA GTCTGCCCTT 
GCTGGTGCCG GTGGCTGCAA AGATGCTCCT GTCCCTGGGG GTCCTGGCCC TGTGCTCAGT 
GACCGGAGGC TGTGCCTTGC CCTGGATGAG CCCCAGCTCT GCAATGGGCA TATGGGGAGT 
GCTTCCCGGC GAGTTGAGAA TGGGGCATGG GCGTACCTCA GCCCTCTGGT GCTGCGTAAA 
GAACTAGAGT CCCTGGTAGA GAATGAGGGC ATCGAGGTGT TGGCGTTGCC TGAACTGCCT 
GCTGCCCACC CTATCATCTT CTGGAACCTC CTGTGGTATT TCCAACGGCT ACGCCTGCCT 
AGTATTCTGC CAGGCCTGGT GTTGGCCTCC TGTAATGGGC CGCCACCCAG CCAGCTCTCC 
CAGGGACCAT CTTCATGGCT CACCCCTGAT CCAGCTTCTG TGCATGTGCG TCTGCTGTGG 
GACGTCCTGA TCCCTGACCC CAATAGCTGC CCGCCTCTCT ACGTGCTCTG GAGGGTCCAC 
AGCCAGATCC CACAGCGGGT GGTGTGGCCA GGTCCAGTAC CCTCCTGTCT TAGCTTGGCA 
TTACTGGAGT CAGTGCTGCG CCATTTCGGA CTCAACGAGG TTCATAAAGC TGTTGGACTT 
CTGTTGGAGA CTCTAGGGCC CCCTCCCACT GGCCTGCACC TGCAGAGGGG AATCTACCGT 
GAGATCTTAT TCCTGACGAT GGCTGCTCTG GGCAAGGACC ACGTGGATAT AGTGGCCTTC 
GACAAGAAGT ACAAGTCTGC CTTTAACAAG CTGGCCAGCA GCATGGGCAA GGAAGAGCTG 
AGGCAGCGGC GGGCACAGAT GCCTTCCCCC AAAGCCATCG ACTGCCGAAA GTGTTTTGGA 
GCACCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759472.1
CDS	589..4986
Translation	MEHLYLRRRGFLSLLGPCALPGQLNLSQMWQSSLGHWAKRCPRAIHAFRLLLVVTPWSSVLGSWVELNQSSATAGGVTSPPLLSWGYYMTGKNDLSLGSKCLTRRHIATQPTWPLQALGTPAPTSCTGVQQKGQVCMPWASLTSAWFCPARWGPAVFLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVENLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSTRHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRVAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSNYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVWGAAQFRQPLKDRRRLQQEQQEQQQQQQQQQQQVAEQQKSGSSQTEPCLERPSPTRPLQRQTTWAGRSLREPSSPIGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELAPGSSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEAGTPRKGLGARLQQLLTPSRRSSASRISVPELPPDLPPAARRSPMDSLLWPRERPGSTASEVSSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKSALAGAGGCKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGIEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASCNGPPPSQLSQGPSSWLTPDPASVHVRLLWDVLIPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHFGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPSPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_008761250.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761250.2

ORF Insert Sequence:

ATGGAGCACC TATACCTGAG GAGACGTGGG TTCCTGAGCC TACTGGGCCC CTGCGCCCTC 
CCCGGCCAGC TGAACCTATC ACAGATGTGG CAGTCATCGC TAGGGCACTG GGCGAAGAGG 
TGCCCCAGGG CTATACATGC ATTCAGACTT CTGCTGGTGG TCACCCCTTG GAGCTCAGTG 
CTGGGCTCCT GGGTGGAACT CAACCAGTCA TCTGCTACCG CAGGGGGCGT GACAAGCCCC 
CCCTTGTTGA GCTGGGGGTA TTATATGACG GGAAAGAACG ACCTAAGCCT GGGTTCCAAG 
TGCTTGACAC GACGCCATAT AGCCACTCAG CCAACCTGGC CCCTCCAGGC CCTGGGCACC 
CCCGCACCTA CCTCATGTAC CGGCGTGCAG CAGAAGGGGC AGGTCTGCAT GCCCTGGGCA 
TCACTGACCT CTGCCTGGTT CTGCCCAGCA AGGTGGGGCC CAGCAGTGTT CCTGTGCTAC 
AAGGTGGGCC TGGCGAAGGC CAACACACTG GTGTATGAGG CAGAGCTGCT AGGCCGCTAC 
CCAGAGGAAG ACAATGAGGC GTTTCCGCTG CCTGAGTCAG TGCCAGTCTT CTGCCTGCCC 
ATGGGAGCCA CTATCGAGTG CTGGCCTGCC CAGACCAAGT ACCCAGTACC CGTCTTTTCC 
ACCTTTGTGC TCACGGGAGC TGCTGGTGAT AAGGTGTATG GTGCCGCGTT GCAGTTCTAT 
GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG AGGCAGGCAC GGGCACTGGG CCTCCTGAGT 
GCCGTGGAAC GGGGACGGGC CCTGGGAGGC CGAGCAGTGC GCAGTCGGAG AGCCATTGCT 
GTGCTGTCCC GCTGGCCCGC CTTCCCGGCC TTCCGAGCCT TCCTCACCTT CCTTTACCGC 
TACTCCGTCT CAGGCCCCCA TCGTCTGCCC CTGGAAGCGC ACATCTCCCA CTTCATTCAC 
AACGTCCCGT TCCCTTCCCC ACAGAGACCA CGCATCCTAG TGCAGATGTC TCCCTATGAC 
AACCTGCTCC TCTGTCAACC TGTCTCCTCA CCCCTGCCCC TGAGTGGTGC TAGCTTTTTA 
CAGCTGCTGC AGAACCTGGG CCCAGAATTG GCTATTACAT TGCTGCTGGC TGTGCTCACA 
GAGCACAAAC TGCTGGTCCA CTCTCTGCGG CCAGATCTGC TTACCAGTGT CTGCGAGGCC 
CTTGTCTCAA TGATTTTCCC TCTGCACTGG CAGTGCCCCT ACATTCCGCT GTGCCCGCTA 
GTGCTGGCGG ATGTGCTGAG TGCCCCTGTG CCCTTCATTG TGGGTATCCA CTCCAGTTAT 
TTCGATCTGC ATGACCCACC TGCTGACGTC ATCTGTGTTG ATCTTGACAC CAACACGCTC 
TTTCAAACGG AGGAAAAGAA GCCCCTCTCT GCTAGGACCC TGCCCCGAAG ACCTTACAAG 
CTTCTGCTGG CCACACTGAC GAATCTGTAC CAGCAGCTGG ACCAGACTTA CACTGGACCA 
GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG ACAGACTATG AGGCAGTGTG TGGCCGCAGG 
ACTCGCCTAG AACGGGAAGT CCAGGGAGCC TTCCTGCGCT TCATGGCCTG TCTGCTCAAA 
GGCTACCGGA ACTTCCTTCG ACCTCTCACC CAGGCCCCCT CTGAGGGGTC TCGAGATGTT 
GAGAACCTTT TCTACCTTCA GGGTTTCCTT AAGTCCCGGG AACGCTCCAG TCACAAGCTG 
TACTCTCAGC TCCTACACAC GCAGATGTTC TCCCAGTTCA TCGAGGAATG CTCTTTCGGC 
TCTACCCGGC ATGCAGCCCT GGAATTCTTT GACTCTTGTG TTGACAAGGT TCACCCAGAG 
CAGGAGAAGC CTGAGCCAAC ACCCTTAGTG GAGCTGGAGG AACTATCGGG AAGTGAGCTC 
ACTGTCTTTA TCACACCCCC AGAGGAACCG CCAGTACTGG AAGGCAGTGA ATCTACTCCT 
CAGTACTGTT ATGATGGGTT TCCAGAGCTG AAGGCTGAGC TATTTGAGTC TCCTCAGGAA 
CAACAGGGGG CACTCCCTGT GCCAGGCCCA TCCCGTAGTG CCCCCAGCAG TCCTGCACCT 
CGCCGTACCA AACAGGAGAT GAAGGTTGCA CAGCGCGTGG CACAGAAGTC AGCCACTGTA 
CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT CACTGCTATG GACTGTGGTT CTTATGCCTA 
CCTGCCTATG TGCGTTCTGC ACCTTCCCGG GTGCGAGCAT TACACACAGC CTACCATGTA 
TTACGTGAGA TGGAGAGCCG CAAGGTGGTG CTCCCTGATG AGGTCTGTTA CCGGGTACTG 
ATGCAGCTCT GCTCAAACTA TGGGCAACCC GTGTTGTCTG TGCGAGTCAT GCTGGAGATG 
CGACGGGCAG GCATTGTGCC CAACACCATC ACCTATGGCT ACTACAACAA GGCTGTGCTG 
GAAAGCAAAT GGCCATCTGG TACTCCGGGT GGACGCCTAC GCTGGGCCAA GCTCCGGAAT 
GTTGTCTGGG GGGCGGCTCA GTTCCGCCAG CCCTTAAAGG ACCGAAGGCG GCTGCAGCAG 
GAGCAGCAGG AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AACAGGTGGC CGAGCAGCAA 
AAGTCGGGCA GCTCTCAGAC AGAGCCCTGT CTGGAGCGTC CTTCCCCAAC CCGCCCTCTT 
CAGCGCCAGA CTACTTGGGC TGGACGCAGT CTACGGGAGC CATCTTCACC CATCGGGCGC 
CTGGTCAAGA GCGGCAGCCT AGGCAGCGCC CGCGGGGCAC AGCCCACTGT GGAGGCTGGC 
GTGGCTCACA TGATAGAGGC CTTGGGGGTC CTTGAACCCC GTGGATCACC TGTGCCTTGG 
CAGGATGGAA GTCTCTCAGA CCTGAGTCTG ACAGGGGAAG AACTGGCACC TGGAAGCAGC 
CCAGGGGGCT CAGGCTCAGC CCTGAGTGCC CAGTCCACTG AGGCCCTAGA AGGGATCAGT 
GGGCGGGGTT CCAAAACTAG TGGATGTCAG GAGGAGGCGG GCACCCCCAG AAAAGGGCTG 
GGTGCCCGCC TCCAACAGCT GCTTACCCCT TCCCGCCGCT CCTCTGCTTC CCGGATCTCC 
GTACCTGAGT TGCCCCCTGA CCTGCCTCCT GCGGCTCGCC GGAGTCCCAT GGATAGCCTT 
CTGTGGCCCC GAGAACGCCC TGGATCCACT GCTTCTGAGG TAAGTTCTGC TTCTCTGGGC 
AGTGAGTGGG ATATCTCAGA GTCTTCTCTC AGCAGCCTGA GTCTTCGACG TTCCTCAGAG 
CGTCTCAGCG ACACCCCTGG AGCTTTCCAA CCACCCTCCC TAGAAATTCT GATGTCTAGC 
TGCTCCCTGT GCCATGCCTG TGATTCCCTG GTGTATGATG AGGAAATCAT GGCCGGCTGG 
GCACCGGATG ACTCTAACCT CAATACGACT TGTCCCTTCT GCGCCTGTCC CTTCGTGCCC 
CTGCTTAGTG TCCAGACTCT TGATTCTCGA CCCAGTGCGC CTAGTCCAAA GTCTGCCCTT 
GCTGGTGCCG GTGGCTGCAA AGATGCTCCT GTCCCTGGGG GTCCTGGCCC TGTGCTCAGT 
GACCGGAGGC TGTGCCTTGC CCTGGATGAG CCCCAGCTCT GCAATGGGCA TATGGGGAGT 
GCTTCCCGGC GAGTTGAGAA TGGGGCATGG GCGTACCTCA GCCCTCTGGT GCTGCGTAAA 
GAACTAGAGT CCCTGGTAGA GAATGAGGGC ATCGAGGTGT TGGCGTTGCC TGAACTGCCT 
GCTGCCCACC CTATCATCTT CTGGAACCTC CTGTGGTATT TCCAACGGCT ACGCCTGCCT 
AGTATTCTGC CAGGCCTGGT GTTGGCCTCC TGTAATGGGC CGCCACCCAG CCAGCTCTCC 
CAGGGACCAT CTTCATGGCT CACCCCTGAT CCAGCTTCTG TGCATGTGCG TCTGCTGTGG 
GACGTCCTGA TCCCTGACCC CAATAGCTGC CCGCCTCTCT ACGTGCTCTG GAGGGTCCAC 
AGCCAGATCC CACAGCGGGT GGTGTGGCCA GGTCCAGTAC CCTCCTGTCT TAGCTTGGCA 
TTACTGGAGT CAGTGCTGCG CCATTTCGGA CTCAACGAGG TTCATAAAGC TGTTGGACTT 
CTGTTGGAGA CTCTAGGGCC CCCTCCCACT GGCCTGCACC TGCAGAGGGG AATCTACCGT 
GAGATCTTAT TCCTGACGAT GGCTGCTCTG GGCAAGGACC ACGTGGATAT AGTGGCCTTC 
GACAAGAAGT ACAAGTCTGC CTTTAACAAG CTGGCCAGCA GCATGGGCAA GGAAGAGCTG 
AGGCAGCGGC GGGCACAGAT GCCTTCCCCC AAAGCCATCG ACTGCCGAAA GTGTTTTGGA 
GCACCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_008761248.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759470.1 Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.

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CloneID	ORa48093
Clone ID Related Accession (Same CDS sequence)	XM_008761248.2
Accession Version	XM_008761248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAAGGGGGG GCCATGGCAG AGGAGCGGCC TCCCCGGTTG 
GTGGATTACT TCGTGGTGGC TGGGCTTGCA GGAAATGGAG CACCTATACC TGAGGAGACG 
TGGGTTCCTG AGCCTACTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAACC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAA GAGGTGCCCC AGGGCTATAC ATGCATTCAG 
ACTTCTGCTG GTGGTCACCC CTTGGAGCTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGCAGGGG GCGTGACAAG CCCCCCCTTG TTGAGCTGGG GGTATTATAT 
GACGGGAAAG AACGACCTAA GCCTGGGTTC CAAGTGCTTG ACACGACGCC ATATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAT GTACCGGCGT 
GCAGCAGAAG GGGCAGGTCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTTCTGCCC 
AGCAAGGGTG AGAGCACTCC TCATACTTAC TGCCGGTTAC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTT CCTGTGCTAC AAGGTGGGCC TGGCGAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT AGGCCGCTAC CCAGAGGAAG ACAATGAGGC GTTTCCGCTG 
CCTGAGTCAG TGCCAGTCTT CTGCCTGCCC ATGGGAGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCAGTACC CGTCTTTTCC ACCTTTGTGC TCACGGGAGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCGTT GCAGTTCTAT GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTCCTGAGT GCCGTGGAAC GGGGACGGGC CCTGGGAGGC 
CGAGCAGTGC GCAGTCGGAG AGCCATTGCT GTGCTGTCCC GCTGGCCCGC CTTCCCGGCC 
TTCCGAGCCT TCCTCACCTT CCTTTACCGC TACTCCGTCT CAGGCCCCCA TCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCGT TCCCTTCCCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGTCAACC TGTCTCCTCA 
CCCCTGCCCC TGAGTGGTGC TAGCTTTTTA CAGCTGCTGC AGAACCTGGG CCCAGAATTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCTCTGCGG 
CCAGATCTGC TTACCAGTGT CTGCGAGGCC CTTGTCTCAA TGATTTTCCC TCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTA GTGCTGGCGG ATGTGCTGAG TGCCCCTGTG 
CCCTTCATTG TGGGTATCCA CTCCAGTTAT TTCGATCTGC ATGACCCACC TGCTGACGTC 
ATCTGTGTTG ATCTTGACAC CAACACGCTC TTTCAAACGG AGGAAAAGAA GCCCCTCTCT 
GCTAGGACCC TGCCCCGAAG ACCTTACAAG CTTCTGCTGG CCACACTGAC GAATCTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTATG AGGCAGTGTG TGGCCGCAGG ACTCGCCTAG AACGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGA ACTTCCTTCG ACCTCTCACC 
CAGGCCCCCT CTGAGGGGTC TCGAGATGTT GAGAACCTTT TCTACCTTCA GGGTTTCCTT 
AAGTCCCGGG AACGCTCCAG TCACAAGCTG TACTCTCAGC TCCTACACAC GCAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTCGGC TCTACCCGGC ATGCAGCCCT GGAATTCTTT 
GACTCTTGTG TTGACAAGGT TCACCCAGAG CAGGAGAAGC CTGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AACTATCGGG AAGTGAGCTC ACTGTCTTTA TCACACCCCC AGAGGAACCG 
CCAGTACTGG AAGGCAGTGA ATCTACTCCT CAGTACTGTT ATGATGGGTT TCCAGAGCTG 
AAGGCTGAGC TATTTGAGTC TCCTCAGGAA CAACAGGGGG CACTCCCTGT GCCAGGCCCA 
TCCCGTAGTG CCCCCAGCAG TCCTGCACCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGCGTGG CACAGAAGTC AGCCACTGTA CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT 
CACTGCTATG GACTGTGGTT CTTATGCCTA CCTGCCTATG TGCGTTCTGC ACCTTCCCGG 
GTGCGAGCAT TACACACAGC CTACCATGTA TTACGTGAGA TGGAGAGCCG CAAGGTGGTG 
CTCCCTGATG AGGTCTGTTA CCGGGTACTG ATGCAGCTCT GCTCAAACTA TGGGCAACCC 
GTGTTGTCTG TGCGAGTCAT GCTGGAGATG CGACGGGCAG GCATTGTGCC CAACACCATC 
ACCTATGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAAT GGCCATCTGG TACTCCGGGT 
GGACGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCTGGG GGGCGGCTCA GTTCCGCCAG 
CCCTTAAAGG ACCGAAGGCG GCTGCAGCAG GAGCAGCAGG AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGGTGGC CGAGCAGCAA AAGTCGGGCA GCTCTCAGAC AGAGCCCTGT 
CTGGAGCGTC CTTCCCCAAC CCGCCCTCTT CAGCGCCAGA CTACTTGGGC TGGACGCAGT 
CTACGGGAGC CATCTTCACC CATCGGGCGC CTGGTCAAGA GCGGCAGCCT AGGCAGCGCC 
CGCGGGGCAC AGCCCACTGT GGAGGCTGGC GTGGCTCACA TGATAGAGGC CTTGGGGGTC 
CTTGAACCCC GTGGATCACC TGTGCCTTGG CAGGATGGAA GTCTCTCAGA CCTGAGTCTG 
ACAGGGGAAG AACTGGCACC TGGAAGCAGC CCAGGGGGCT CAGGCTCAGC CCTGAGTGCC 
CAGTCCACTG AGGCCCTAGA AGGGATCAGT GGGCGGGGTT CCAAAACTAG TGGATGTCAG 
GAGGAGGCGG GCACCCCCAG AAAAGGGCTG GGTGCCCGCC TCCAACAGCT GCTTACCCCT 
TCCCGCCGCT CCTCTGCTTC CCGGATCTCC GTACCTGAGT TGCCCCCTGA CCTGCCTCCT 
GCGGCTCGCC GGAGTCCCAT GGATAGCCTT CTGTGGCCCC GAGAACGCCC TGGATCCACT 
GCTTCTGAGG TAAGTTCTGC TTCTCTGGGC AGTGAGTGGG ATATCTCAGA GTCTTCTCTC 
AGCAGCCTGA GTCTTCGACG TTCCTCAGAG CGTCTCAGCG ACACCCCTGG AGCTTTCCAA 
CCACCCTCCC TAGAAATTCT GATGTCTAGC TGCTCCCTGT GCCATGCCTG TGATTCCCTG 
GTGTATGATG AGGAAATCAT GGCCGGCTGG GCACCGGATG ACTCTAACCT CAATACGACT 
TGTCCCTTCT GCGCCTGTCC CTTCGTGCCC CTGCTTAGTG TCCAGACTCT TGATTCTCGA 
CCCAGTGCGC CTAGTCCAAA GTCTGCCCTT GCTGGTGCCG GTGGCTGCAA AGATGCTCCT 
GTCCCTGGGG GTCCTGGCCC TGTGCTCAGT GACCGGAGGC TGTGCCTTGC CCTGGATGAG 
CCCCAGCTCT GCAATGGGCA TATGGGGAGT GCTTCCCGGC GAGTTGAGAA TGGGGCATGG 
GCGTACCTCA GCCCTCTGGT GCTGCGTAAA GAACTAGAGT CCCTGGTAGA GAATGAGGGC 
ATCGAGGTGT TGGCGTTGCC TGAACTGCCT GCTGCCCACC CTATCATCTT CTGGAACCTC 
CTGTGGTATT TCCAACGGCT ACGCCTGCCT AGTATTCTGC CAGGCCTGGT GTTGGCCTCC 
TGTAATGGGC CGCCACCCAG CCAGCTCTCC CAGGGACCAT CTTCATGGCT CACCCCTGAT 
CCAGCTTCTG TGCATGTGCG TCTGCTGTGG GACGTCCTGA TCCCTGACCC CAATAGCTGC 
CCGCCTCTCT ACGTGCTCTG GAGGGTCCAC AGCCAGATCC CACAGCGGGT GGTGTGGCCA 
GGTCCAGTAC CCTCCTGTCT TAGCTTGGCA TTACTGGAGT CAGTGCTGCG CCATTTCGGA 
CTCAACGAGG TTCATAAAGC TGTTGGACTT CTGTTGGAGA CTCTAGGGCC CCCTCCCACT 
GGCCTGCACC TGCAGAGGGG AATCTACCGT GAGATCTTAT TCCTGACGAT GGCTGCTCTG 
GGCAAGGACC ACGTGGATAT AGTGGCCTTC GACAAGAAGT ACAAGTCTGC CTTTAACAAG 
CTGGCCAGCA GCATGGGCAA GGAAGAGCTG AGGCAGCGGC GGGCACAGAT GCCTTCCCCC 
AAAGCCATCG ACTGCCGAAA GTGTTTTGGA GCACCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759470.1
CDS	1052..5599
Translation	MEADAVSEGGAMAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYDGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGESTPHTYCRLPRNLNPGMWGPAVFLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVENLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSTRHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRVAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSNYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVWGAAQFRQPLKDRRRLQQEQQEQQQQQQQQQQQVAEQQKSGSSQTEPCLERPSPTRPLQRQTTWAGRSLREPSSPIGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELAPGSSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEAGTPRKGLGARLQQLLTPSRRSSASRISVPELPPDLPPAARRSPMDSLLWPRERPGSTASEVSSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKSALAGAGGCKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGIEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASCNGPPPSQLSQGPSSWLTPDPASVHVRLLWDVLIPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHFGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPSPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_008761248.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761248.2

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAAGGGGGG GCCATGGCAG AGGAGCGGCC TCCCCGGTTG 
GTGGATTACT TCGTGGTGGC TGGGCTTGCA GGAAATGGAG CACCTATACC TGAGGAGACG 
TGGGTTCCTG AGCCTACTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAACC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAA GAGGTGCCCC AGGGCTATAC ATGCATTCAG 
ACTTCTGCTG GTGGTCACCC CTTGGAGCTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGCAGGGG GCGTGACAAG CCCCCCCTTG TTGAGCTGGG GGTATTATAT 
GACGGGAAAG AACGACCTAA GCCTGGGTTC CAAGTGCTTG ACACGACGCC ATATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAT GTACCGGCGT 
GCAGCAGAAG GGGCAGGTCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTTCTGCCC 
AGCAAGGGTG AGAGCACTCC TCATACTTAC TGCCGGTTAC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTT CCTGTGCTAC AAGGTGGGCC TGGCGAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT AGGCCGCTAC CCAGAGGAAG ACAATGAGGC GTTTCCGCTG 
CCTGAGTCAG TGCCAGTCTT CTGCCTGCCC ATGGGAGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCAGTACC CGTCTTTTCC ACCTTTGTGC TCACGGGAGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCGTT GCAGTTCTAT GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTCCTGAGT GCCGTGGAAC GGGGACGGGC CCTGGGAGGC 
CGAGCAGTGC GCAGTCGGAG AGCCATTGCT GTGCTGTCCC GCTGGCCCGC CTTCCCGGCC 
TTCCGAGCCT TCCTCACCTT CCTTTACCGC TACTCCGTCT CAGGCCCCCA TCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCGT TCCCTTCCCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGTCAACC TGTCTCCTCA 
CCCCTGCCCC TGAGTGGTGC TAGCTTTTTA CAGCTGCTGC AGAACCTGGG CCCAGAATTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCTCTGCGG 
CCAGATCTGC TTACCAGTGT CTGCGAGGCC CTTGTCTCAA TGATTTTCCC TCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTA GTGCTGGCGG ATGTGCTGAG TGCCCCTGTG 
CCCTTCATTG TGGGTATCCA CTCCAGTTAT TTCGATCTGC ATGACCCACC TGCTGACGTC 
ATCTGTGTTG ATCTTGACAC CAACACGCTC TTTCAAACGG AGGAAAAGAA GCCCCTCTCT 
GCTAGGACCC TGCCCCGAAG ACCTTACAAG CTTCTGCTGG CCACACTGAC GAATCTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTATG AGGCAGTGTG TGGCCGCAGG ACTCGCCTAG AACGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGA ACTTCCTTCG ACCTCTCACC 
CAGGCCCCCT CTGAGGGGTC TCGAGATGTT GAGAACCTTT TCTACCTTCA GGGTTTCCTT 
AAGTCCCGGG AACGCTCCAG TCACAAGCTG TACTCTCAGC TCCTACACAC GCAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTCGGC TCTACCCGGC ATGCAGCCCT GGAATTCTTT 
GACTCTTGTG TTGACAAGGT TCACCCAGAG CAGGAGAAGC CTGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AACTATCGGG AAGTGAGCTC ACTGTCTTTA TCACACCCCC AGAGGAACCG 
CCAGTACTGG AAGGCAGTGA ATCTACTCCT CAGTACTGTT ATGATGGGTT TCCAGAGCTG 
AAGGCTGAGC TATTTGAGTC TCCTCAGGAA CAACAGGGGG CACTCCCTGT GCCAGGCCCA 
TCCCGTAGTG CCCCCAGCAG TCCTGCACCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGCGTGG CACAGAAGTC AGCCACTGTA CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT 
CACTGCTATG GACTGTGGTT CTTATGCCTA CCTGCCTATG TGCGTTCTGC ACCTTCCCGG 
GTGCGAGCAT TACACACAGC CTACCATGTA TTACGTGAGA TGGAGAGCCG CAAGGTGGTG 
CTCCCTGATG AGGTCTGTTA CCGGGTACTG ATGCAGCTCT GCTCAAACTA TGGGCAACCC 
GTGTTGTCTG TGCGAGTCAT GCTGGAGATG CGACGGGCAG GCATTGTGCC CAACACCATC 
ACCTATGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAAT GGCCATCTGG TACTCCGGGT 
GGACGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCTGGG GGGCGGCTCA GTTCCGCCAG 
CCCTTAAAGG ACCGAAGGCG GCTGCAGCAG GAGCAGCAGG AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGGTGGC CGAGCAGCAA AAGTCGGGCA GCTCTCAGAC AGAGCCCTGT 
CTGGAGCGTC CTTCCCCAAC CCGCCCTCTT CAGCGCCAGA CTACTTGGGC TGGACGCAGT 
CTACGGGAGC CATCTTCACC CATCGGGCGC CTGGTCAAGA GCGGCAGCCT AGGCAGCGCC 
CGCGGGGCAC AGCCCACTGT GGAGGCTGGC GTGGCTCACA TGATAGAGGC CTTGGGGGTC 
CTTGAACCCC GTGGATCACC TGTGCCTTGG CAGGATGGAA GTCTCTCAGA CCTGAGTCTG 
ACAGGGGAAG AACTGGCACC TGGAAGCAGC CCAGGGGGCT CAGGCTCAGC CCTGAGTGCC 
CAGTCCACTG AGGCCCTAGA AGGGATCAGT GGGCGGGGTT CCAAAACTAG TGGATGTCAG 
GAGGAGGCGG GCACCCCCAG AAAAGGGCTG GGTGCCCGCC TCCAACAGCT GCTTACCCCT 
TCCCGCCGCT CCTCTGCTTC CCGGATCTCC GTACCTGAGT TGCCCCCTGA CCTGCCTCCT 
GCGGCTCGCC GGAGTCCCAT GGATAGCCTT CTGTGGCCCC GAGAACGCCC TGGATCCACT 
GCTTCTGAGG TAAGTTCTGC TTCTCTGGGC AGTGAGTGGG ATATCTCAGA GTCTTCTCTC 
AGCAGCCTGA GTCTTCGACG TTCCTCAGAG CGTCTCAGCG ACACCCCTGG AGCTTTCCAA 
CCACCCTCCC TAGAAATTCT GATGTCTAGC TGCTCCCTGT GCCATGCCTG TGATTCCCTG 
GTGTATGATG AGGAAATCAT GGCCGGCTGG GCACCGGATG ACTCTAACCT CAATACGACT 
TGTCCCTTCT GCGCCTGTCC CTTCGTGCCC CTGCTTAGTG TCCAGACTCT TGATTCTCGA 
CCCAGTGCGC CTAGTCCAAA GTCTGCCCTT GCTGGTGCCG GTGGCTGCAA AGATGCTCCT 
GTCCCTGGGG GTCCTGGCCC TGTGCTCAGT GACCGGAGGC TGTGCCTTGC CCTGGATGAG 
CCCCAGCTCT GCAATGGGCA TATGGGGAGT GCTTCCCGGC GAGTTGAGAA TGGGGCATGG 
GCGTACCTCA GCCCTCTGGT GCTGCGTAAA GAACTAGAGT CCCTGGTAGA GAATGAGGGC 
ATCGAGGTGT TGGCGTTGCC TGAACTGCCT GCTGCCCACC CTATCATCTT CTGGAACCTC 
CTGTGGTATT TCCAACGGCT ACGCCTGCCT AGTATTCTGC CAGGCCTGGT GTTGGCCTCC 
TGTAATGGGC CGCCACCCAG CCAGCTCTCC CAGGGACCAT CTTCATGGCT CACCCCTGAT 
CCAGCTTCTG TGCATGTGCG TCTGCTGTGG GACGTCCTGA TCCCTGACCC CAATAGCTGC 
CCGCCTCTCT ACGTGCTCTG GAGGGTCCAC AGCCAGATCC CACAGCGGGT GGTGTGGCCA 
GGTCCAGTAC CCTCCTGTCT TAGCTTGGCA TTACTGGAGT CAGTGCTGCG CCATTTCGGA 
CTCAACGAGG TTCATAAAGC TGTTGGACTT CTGTTGGAGA CTCTAGGGCC CCCTCCCACT 
GGCCTGCACC TGCAGAGGGG AATCTACCGT GAGATCTTAT TCCTGACGAT GGCTGCTCTG 
GGCAAGGACC ACGTGGATAT AGTGGCCTTC GACAAGAAGT ACAAGTCTGC CTTTAACAAG 
CTGGCCAGCA GCATGGGCAA GGAAGAGCTG AGGCAGCGGC GGGCACAGAT GCCTTCCCCC 
AAAGCCATCG ACTGCCGAAA GTGTTTTGGA GCACCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_342287.9 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_342288.6 Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.

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CloneID	ORa48094
Clone ID Related Accession (Same CDS sequence)	XM_342287.9
Accession Version	XM_342287.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_342287.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAAGGGGGG GCCATGGCAG AGGAGCGGCC TCCCCGGTTG 
GTGGATTACT TCGTGGTGGC TGGGCTTGCA GGAAATGGAG CACCTATACC TGAGGAGACG 
TGGGTTCCTG AGCCTACTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAACC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAA GAGGTGCCCC AGGGCTATAC ATGCATTCAG 
ACTTCTGCTG GTGGTCACCC CTTGGAGCTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGCAGGGG GCGTGACAAG CCCCCCCTTG TTGAGCTGGG GGTATTATAT 
GACGGGAAAG AACGACCTAA GCCTGGGTTC CAAGTGCTTG ACACGACGCC ATATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAT GTACCGGCGT 
GCAGCAGAAG GGGCAGGTCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTTCTGCCC 
AGCAAGGGTG AGAGCACTCC TCATACTTAC TGCCGGTTAC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTT CCTGTGCTAC AAGGTGGGCC TGGCGAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT AGGCCGCTAC CCAGAGGAAG ACAATGAGGC GTTTCCGCTG 
CCTGAGTCAG TGCCAGTCTT CTGCCTGCCC ATGGGAGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCAGTACC CGTCTTTTCC ACCTTTGTGC TCACGGGAGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCGTT GCAGTTCTAT GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTCCTGAGT GCCGTGGAAC GGGGACGGGC CCTGGGAGGC 
CGAGCAGTGC GCAGTCGGAG AGCCATTGCT GTGCTGTCCC GCTGGCCCGC CTTCCCGGCC 
TTCCGAGCCT TCCTCACCTT CCTTTACCGC TACTCCGTCT CAGGCCCCCA TCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCGT TCCCTTCCCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGTCAACC TGTCTCCTCA 
CCCCTGCCCC TGAGTGGTGC TAGCTTTTTA CAGCTGCTGC AGAACCTGGG CCCAGAATTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCTCTGCGG 
CCAGATCTGC TTACCAGTGT CTGCGAGGCC CTTGTCTCAA TGATTTTCCC TCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTA GTGCTGGCGG ATGTGCTGAG TGCCCCTGTG 
CCCTTCATTG TGGGTATCCA CTCCAGTTAT TTCGATCTGC ATGACCCACC TGCTGACGTC 
ATCTGTGTTG ATCTTGACAC CAACACGCTC TTTCAAACGG AGGAAAAGAA GCCCCTCTCT 
GCTAGGACCC TGCCCCGAAG ACCTTACAAG CTTCTGCTGG CCACACTGAC GAATCTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTATG AGGCAGTGTG TGGCCGCAGG ACTCGCCTAG AACGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGA ACTTCCTTCG ACCTCTCACC 
CAGGCCCCCT CTGAGGGGTC TCGAGATGTT GAGAACCTTT TCTACCTTCA GGGTTTCCTT 
AAGTCCCGGG AACGCTCCAG TCACAAGCTG TACTCTCAGC TCCTACACAC GCAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTCGGC TCTACCCGGC ATGCAGCCCT GGAATTCTTT 
GACTCTTGTG TTGACAAGGT TCACCCAGAG CAGGAGAAGC CTGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AACTATCGGG AAGTGAGCTC ACTGTCTTTA TCACACCCCC AGAGGAACCG 
CCAGTACTGG AAGGCAGTGA ATCTACTCCT CAGTACTGTT ATGATGGGTT TCCAGAGCTG 
AAGGCTGAGC TATTTGAGTC TCCTCAGGAA CAACAGGGGG CACTCCCTGT GCCAGGCCCA 
TCCCGTAGTG CCCCCAGCAG TCCTGCACCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGCGTGG CACAGAAGTC AGCCACTGTA CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT 
CACTGCTATG GACTGTGGTT CTTATGCCTA CCTGCCTATG TGCGTTCTGC ACCTTCCCGG 
GTGCGAGCAT TACACACAGC CTACCATGTA TTACGTGAGA TGGAGAGCCG CAAGGTGGTG 
CTCCCTGATG AGGTCTGTTA CCGGGTACTG ATGCAGCTCT GCTCAAACTA TGGGCAACCC 
GTGTTGTCTG TGCGAGTCAT GCTGGAGATG CGACGGGCAG GCATTGTGCC CAACACCATC 
ACCTATGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAAT GGCCATCTGG TACTCCGGGT 
GGACGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCTGGG GGGCGGCTCA GTTCCGCCAG 
CCCTTAAAGG ACCGAAGGCG GCTGCAGCAG GAGCAGCAGG AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGGTGGC CGAGCAGCAA AAGTCGGGCA GCTCTCAGAC AGAGCCCTGT 
CTGGAGCGTC CTTCCCCAAC CCGCCCTCTT CAGCGCCAGA CTACTTGGGC TGGACGCAGT 
CTACGGGAGC CATCTTCACC CATCGGGCGC CTGGTCAAGA GCGGCAGCCT AGGCAGCGCC 
CGCGGGGCAC AGCCCACTGT GGAGGCTGGC GTGGCTCACA TGATAGAGGC CTTGGGGGTC 
CTTGAACCCC GTGGATCACC TGTGCCTTGG CAGGATGGAA GTCTCTCAGA CCTGAGTCTG 
ACAGGGGAAG AACTGGCACC TGGAAGCAGC CCAGGGGGCT CAGGCTCAGC CCTGAGTGCC 
CAGTCCACTG AGGCCCTAGA AGGGATCAGT GGGCGGGGTT CCAAAACTAG TGGATGTCAG 
GAGGAGGCGG GCACCCCCAG AAAAGGGCTG GGTGCCCGCC TCCAACAGCT GCTTACCCCT 
TCCCGCCGCT CCTCTGCTTC CCGGATCTCC GTACCTGAGT TGCCCCCTGA CCTGCCTCCT 
GCGGCTCGCC GGAGTCCCAT GGATAGCCTT CTGTGGCCCC GAGAACGCCC TGGATCCACT 
GCTTCTGAGA GTTCTGCTTC TCTGGGCAGT GAGTGGGATA TCTCAGAGTC TTCTCTCAGC 
AGCCTGAGTC TTCGACGTTC CTCAGAGCGT CTCAGCGACA CCCCTGGAGC TTTCCAACCA 
CCCTCCCTAG AAATTCTGAT GTCTAGCTGC TCCCTGTGCC ATGCCTGTGA TTCCCTGGTG 
TATGATGAGG AAATCATGGC CGGCTGGGCA CCGGATGACT CTAACCTCAA TACGACTTGT 
CCCTTCTGCG CCTGTCCCTT CGTGCCCCTG CTTAGTGTCC AGACTCTTGA TTCTCGACCC 
AGTGCGCCTA GTCCAAAGTC TGCCCTTGCT GGTGCCGGTG GCTGCAAAGA TGCTCCTGTC 
CCTGGGGGTC CTGGCCCTGT GCTCAGTGAC CGGAGGCTGT GCCTTGCCCT GGATGAGCCC 
CAGCTCTGCA ATGGGCATAT GGGGAGTGCT TCCCGGCGAG TTGAGAATGG GGCATGGGCG 
TACCTCAGCC CTCTGGTGCT GCGTAAAGAA CTAGAGTCCC TGGTAGAGAA TGAGGGCATC 
GAGGTGTTGG CGTTGCCTGA ACTGCCTGCT GCCCACCCTA TCATCTTCTG GAACCTCCTG 
TGGTATTTCC AACGGCTACG CCTGCCTAGT ATTCTGCCAG GCCTGGTGTT GGCCTCCTGT 
AATGGGCCGC CACCCAGCCA GCTCTCCCAG GGACCATCTT CATGGCTCAC CCCTGATCCA 
GCTTCTGTGC ATGTGCGTCT GCTGTGGGAC GTCCTGATCC CTGACCCCAA TAGCTGCCCG 
CCTCTCTACG TGCTCTGGAG GGTCCACAGC CAGATCCCAC AGCGGGTGGT GTGGCCAGGT 
CCAGTACCCT CCTGTCTTAG CTTGGCATTA CTGGAGTCAG TGCTGCGCCA TTTCGGACTC 
AACGAGGTTC ATAAAGCTGT TGGACTTCTG TTGGAGACTC TAGGGCCCCC TCCCACTGGC 
CTGCACCTGC AGAGGGGAAT CTACCGTGAG ATCTTATTCC TGACGATGGC TGCTCTGGGC 
AAGGACCACG TGGATATAGT GGCCTTCGAC AAGAAGTACA AGTCTGCCTT TAACAAGCTG 
GCCAGCAGCA TGGGCAAGGA AGAGCTGAGG CAGCGGCGGG CACAGATGCC TTCCCCCAAA 
GCCATCGACT GCCGAAAGTG TTTTGGAGCA CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_342288.6
CDS	1052..5596
Translation	MEADAVSEGGAMAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYDGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGESTPHTYCRLPRNLNPGMWGPAVFLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVENLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSTRHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRVAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSNYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVWGAAQFRQPLKDRRRLQQEQQEQQQQQQQQQQQVAEQQKSGSSQTEPCLERPSPTRPLQRQTTWAGRSLREPSSPIGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELAPGSSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEAGTPRKGLGARLQQLLTPSRRSSASRISVPELPPDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKSALAGAGGCKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGIEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASCNGPPPSQLSQGPSSWLTPDPASVHVRLLWDVLIPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHFGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPSPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_342287.9
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_342287.9

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAAGGGGGG GCCATGGCAG AGGAGCGGCC TCCCCGGTTG 
GTGGATTACT TCGTGGTGGC TGGGCTTGCA GGAAATGGAG CACCTATACC TGAGGAGACG 
TGGGTTCCTG AGCCTACTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAACC TATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAA GAGGTGCCCC AGGGCTATAC ATGCATTCAG 
ACTTCTGCTG GTGGTCACCC CTTGGAGCTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGCAGGGG GCGTGACAAG CCCCCCCTTG TTGAGCTGGG GGTATTATAT 
GACGGGAAAG AACGACCTAA GCCTGGGTTC CAAGTGCTTG ACACGACGCC ATATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAT GTACCGGCGT 
GCAGCAGAAG GGGCAGGTCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTTCTGCCC 
AGCAAGGGTG AGAGCACTCC TCATACTTAC TGCCGGTTAC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTT CCTGTGCTAC AAGGTGGGCC TGGCGAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT AGGCCGCTAC CCAGAGGAAG ACAATGAGGC GTTTCCGCTG 
CCTGAGTCAG TGCCAGTCTT CTGCCTGCCC ATGGGAGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCAGTACC CGTCTTTTCC ACCTTTGTGC TCACGGGAGC TGCTGGTGAT 
AAGGTGTATG GTGCCGCGTT GCAGTTCTAT GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTCCTGAGT GCCGTGGAAC GGGGACGGGC CCTGGGAGGC 
CGAGCAGTGC GCAGTCGGAG AGCCATTGCT GTGCTGTCCC GCTGGCCCGC CTTCCCGGCC 
TTCCGAGCCT TCCTCACCTT CCTTTACCGC TACTCCGTCT CAGGCCCCCA TCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCGT TCCCTTCCCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGTCAACC TGTCTCCTCA 
CCCCTGCCCC TGAGTGGTGC TAGCTTTTTA CAGCTGCTGC AGAACCTGGG CCCAGAATTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCTCTGCGG 
CCAGATCTGC TTACCAGTGT CTGCGAGGCC CTTGTCTCAA TGATTTTCCC TCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCGCTA GTGCTGGCGG ATGTGCTGAG TGCCCCTGTG 
CCCTTCATTG TGGGTATCCA CTCCAGTTAT TTCGATCTGC ATGACCCACC TGCTGACGTC 
ATCTGTGTTG ATCTTGACAC CAACACGCTC TTTCAAACGG AGGAAAAGAA GCCCCTCTCT 
GCTAGGACCC TGCCCCGAAG ACCTTACAAG CTTCTGCTGG CCACACTGAC GAATCTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTATG AGGCAGTGTG TGGCCGCAGG ACTCGCCTAG AACGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGCTACCGGA ACTTCCTTCG ACCTCTCACC 
CAGGCCCCCT CTGAGGGGTC TCGAGATGTT GAGAACCTTT TCTACCTTCA GGGTTTCCTT 
AAGTCCCGGG AACGCTCCAG TCACAAGCTG TACTCTCAGC TCCTACACAC GCAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTCGGC TCTACCCGGC ATGCAGCCCT GGAATTCTTT 
GACTCTTGTG TTGACAAGGT TCACCCAGAG CAGGAGAAGC CTGAGCCAAC ACCCTTAGTG 
GAGCTGGAGG AACTATCGGG AAGTGAGCTC ACTGTCTTTA TCACACCCCC AGAGGAACCG 
CCAGTACTGG AAGGCAGTGA ATCTACTCCT CAGTACTGTT ATGATGGGTT TCCAGAGCTG 
AAGGCTGAGC TATTTGAGTC TCCTCAGGAA CAACAGGGGG CACTCCCTGT GCCAGGCCCA 
TCCCGTAGTG CCCCCAGCAG TCCTGCACCT CGCCGTACCA AACAGGAGAT GAAGGTTGCA 
CAGCGCGTGG CACAGAAGTC AGCCACTGTA CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT 
CACTGCTATG GACTGTGGTT CTTATGCCTA CCTGCCTATG TGCGTTCTGC ACCTTCCCGG 
GTGCGAGCAT TACACACAGC CTACCATGTA TTACGTGAGA TGGAGAGCCG CAAGGTGGTG 
CTCCCTGATG AGGTCTGTTA CCGGGTACTG ATGCAGCTCT GCTCAAACTA TGGGCAACCC 
GTGTTGTCTG TGCGAGTCAT GCTGGAGATG CGACGGGCAG GCATTGTGCC CAACACCATC 
ACCTATGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAAT GGCCATCTGG TACTCCGGGT 
GGACGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCTGGG GGGCGGCTCA GTTCCGCCAG 
CCCTTAAAGG ACCGAAGGCG GCTGCAGCAG GAGCAGCAGG AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAGGTGGC CGAGCAGCAA AAGTCGGGCA GCTCTCAGAC AGAGCCCTGT 
CTGGAGCGTC CTTCCCCAAC CCGCCCTCTT CAGCGCCAGA CTACTTGGGC TGGACGCAGT 
CTACGGGAGC CATCTTCACC CATCGGGCGC CTGGTCAAGA GCGGCAGCCT AGGCAGCGCC 
CGCGGGGCAC AGCCCACTGT GGAGGCTGGC GTGGCTCACA TGATAGAGGC CTTGGGGGTC 
CTTGAACCCC GTGGATCACC TGTGCCTTGG CAGGATGGAA GTCTCTCAGA CCTGAGTCTG 
ACAGGGGAAG AACTGGCACC TGGAAGCAGC CCAGGGGGCT CAGGCTCAGC CCTGAGTGCC 
CAGTCCACTG AGGCCCTAGA AGGGATCAGT GGGCGGGGTT CCAAAACTAG TGGATGTCAG 
GAGGAGGCGG GCACCCCCAG AAAAGGGCTG GGTGCCCGCC TCCAACAGCT GCTTACCCCT 
TCCCGCCGCT CCTCTGCTTC CCGGATCTCC GTACCTGAGT TGCCCCCTGA CCTGCCTCCT 
GCGGCTCGCC GGAGTCCCAT GGATAGCCTT CTGTGGCCCC GAGAACGCCC TGGATCCACT 
GCTTCTGAGA GTTCTGCTTC TCTGGGCAGT GAGTGGGATA TCTCAGAGTC TTCTCTCAGC 
AGCCTGAGTC TTCGACGTTC CTCAGAGCGT CTCAGCGACA CCCCTGGAGC TTTCCAACCA 
CCCTCCCTAG AAATTCTGAT GTCTAGCTGC TCCCTGTGCC ATGCCTGTGA TTCCCTGGTG 
TATGATGAGG AAATCATGGC CGGCTGGGCA CCGGATGACT CTAACCTCAA TACGACTTGT 
CCCTTCTGCG CCTGTCCCTT CGTGCCCCTG CTTAGTGTCC AGACTCTTGA TTCTCGACCC 
AGTGCGCCTA GTCCAAAGTC TGCCCTTGCT GGTGCCGGTG GCTGCAAAGA TGCTCCTGTC 
CCTGGGGGTC CTGGCCCTGT GCTCAGTGAC CGGAGGCTGT GCCTTGCCCT GGATGAGCCC 
CAGCTCTGCA ATGGGCATAT GGGGAGTGCT TCCCGGCGAG TTGAGAATGG GGCATGGGCG 
TACCTCAGCC CTCTGGTGCT GCGTAAAGAA CTAGAGTCCC TGGTAGAGAA TGAGGGCATC 
GAGGTGTTGG CGTTGCCTGA ACTGCCTGCT GCCCACCCTA TCATCTTCTG GAACCTCCTG 
TGGTATTTCC AACGGCTACG CCTGCCTAGT ATTCTGCCAG GCCTGGTGTT GGCCTCCTGT 
AATGGGCCGC CACCCAGCCA GCTCTCCCAG GGACCATCTT CATGGCTCAC CCCTGATCCA 
GCTTCTGTGC ATGTGCGTCT GCTGTGGGAC GTCCTGATCC CTGACCCCAA TAGCTGCCCG 
CCTCTCTACG TGCTCTGGAG GGTCCACAGC CAGATCCCAC AGCGGGTGGT GTGGCCAGGT 
CCAGTACCCT CCTGTCTTAG CTTGGCATTA CTGGAGTCAG TGCTGCGCCA TTTCGGACTC 
AACGAGGTTC ATAAAGCTGT TGGACTTCTG TTGGAGACTC TAGGGCCCCC TCCCACTGGC 
CTGCACCTGC AGAGGGGAAT CTACCGTGAG ATCTTATTCC TGACGATGGC TGCTCTGGGC 
AAGGACCACG TGGATATAGT GGCCTTCGAC AAGAAGTACA AGTCTGCCTT TAACAAGCTG 
GCCAGCAGCA TGGGCAAGGA AGAGCTGAGG CAGCGGCGGG CACAGATGCC TTCCCCCAAA 
GCCATCGACT GCCGAAAGTG TTTTGGAGCA CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_008761249.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759471.1 Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.

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CloneID	ORa48095
Clone ID Related Accession (Same CDS sequence)	XM_008761249.2
Accession Version	XM_008761249.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTCG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCAC CTATACCTGA GGAGACGTGG GTTCCTGAGC CTACTGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT CACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAAGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCTGGTG GTCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTTGTTG AGCTGGGGGT ATTATATGAC GGGAAAGAAC GACCTAAGCC TGGGTTCCAA 
GTGCTTGACA CGACGCCATA TAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCATGTA CCGGCGTGCA GCAGAAGGGG CAGGTCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGA GCACTCCTCA TACTTACTGC 
CGGTTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTTCCT GTGCTACAAG 
GTGGGCCTGG CGAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTAGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCT GAGTCAGTGC CAGTCTTCTG CCTGCCCATG 
GGAGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCCGT CTTTTCCACC 
TTTGTGCTCA CGGGAGCTGC TGGTGATAAG GTGTATGGTG CCGCGTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT CCTGAGTGCC 
GTGGAACGGG GACGGGCCCT GGGAGGCCGA GCAGTGCGCA GTCGGAGAGC CATTGCTGTG 
CTGTCCCGCT GGCCCGCCTT CCCGGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGCTAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCGTTCC CTTCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTGA GTGGTGCTAG CTTTTTACAG 
CTGCTGCAGA ACCTGGGCCC AGAATTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC TCTGCGGCCA GATCTGCTTA CCAGTGTCTG CGAGGCCCTT 
GTCTCAATGA TTTTCCCTCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCTGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGACACCAA CACGCTCTTT 
CAAACGGAGG AAAAGAAGCC CCTCTCTGCT AGGACCCTGC CCCGAAGACC TTACAAGCTT 
CTGCTGGCCA CACTGACGAA TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CAGTGTGTGG CCGCAGGACT 
CGCCTAGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGC 
TACCGGAACT TCCTTCGACC TCTCACCCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAG 
AACCTTTTCT ACCTTCAGGG TTTCCTTAAG TCCCGGGAAC GCTCCAGTCA CAAGCTGTAC 
TCTCAGCTCC TACACACGCA GATGTTCTCC CAGTTCATCG AGGAATGCTC TTTCGGCTCT 
ACCCGGCATG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCTG AGCCAACACC CTTAGTGGAG CTGGAGGAAC TATCGGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCGCCA GTACTGGAAG GCAGTGAATC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTGAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCAC TCCCTGTGCC AGGCCCATCC CGTAGTGCCC CCAGCAGTCC TGCACCTCGC 
CGTACCAAAC AGGAGATGAA GGTTGCACAG CGCGTGGCAC AGAAGTCAGC CACTGTACCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTATGGAC TGTGGTTCTT ATGCCTACCT 
GCCTATGTGC GTTCTGCACC TTCCCGGGTG CGAGCATTAC ACACAGCCTA CCATGTATTA 
CGTGAGATGG AGAGCCGCAA GGTGGTGCTC CCTGATGAGG TCTGTTACCG GGTACTGATG 
CAGCTCTGCT CAAACTATGG GCAACCCGTG TTGTCTGTGC GAGTCATGCT GGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACC TATGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCTGGGGGG CGGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCGGCT GCAGCAGGAG 
CAGCAGGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAAC AGGTGGCCGA GCAGCAAAAG 
TCGGGCAGCT CTCAGACAGA GCCCTGTCTG GAGCGTCCTT CCCCAACCCG CCCTCTTCAG 
CGCCAGACTA CTTGGGCTGG ACGCAGTCTA CGGGAGCCAT CTTCACCCAT CGGGCGCCTG 
GTCAAGAGCG GCAGCCTAGG CAGCGCCCGC GGGGCACAGC CCACTGTGGA GGCTGGCGTG 
GCTCACATGA TAGAGGCCTT GGGGGTCCTT GAACCCCGTG GATCACCTGT GCCTTGGCAG 
GATGGAAGTC TCTCAGACCT GAGTCTGACA GGGGAAGAAC TGGCACCTGG AAGCAGCCCA 
GGGGGCTCAG GCTCAGCCCT GAGTGCCCAG TCCACTGAGG CCCTAGAAGG GATCAGTGGG 
CGGGGTTCCA AAACTAGTGG ATGTCAGGAG GAGGCGGGCA CCCCCAGAAA AGGGCTGGGT 
GCCCGCCTCC AACAGCTGCT TACCCCTTCC CGCCGCTCCT CTGCTTCCCG GATCTCCGTA 
CCTGAGTTGC CCCCTGACCT GCCTCCTGCG GCTCGCCGGA GTCCCATGGA TAGCCTTCTG 
TGGCCCCGAG AACGCCCTGG ATCCACTGCT TCTGAGGTAA GTTCTGCTTC TCTGGGCAGT 
GAGTGGGATA TCTCAGAGTC TTCTCTCAGC AGCCTGAGTC TTCGACGTTC CTCAGAGCGT 
CTCAGCGACA CCCCTGGAGC TTTCCAACCA CCCTCCCTAG AAATTCTGAT GTCTAGCTGC 
TCCCTGTGCC ATGCCTGTGA TTCCCTGGTG TATGATGAGG AAATCATGGC CGGCTGGGCA 
CCGGATGACT CTAACCTCAA TACGACTTGT CCCTTCTGCG CCTGTCCCTT CGTGCCCCTG 
CTTAGTGTCC AGACTCTTGA TTCTCGACCC AGTGCGCCTA GTCCAAAGTC TGCCCTTGCT 
GGTGCCGGTG GCTGCAAAGA TGCTCCTGTC CCTGGGGGTC CTGGCCCTGT GCTCAGTGAC 
CGGAGGCTGT GCCTTGCCCT GGATGAGCCC CAGCTCTGCA ATGGGCATAT GGGGAGTGCT 
TCCCGGCGAG TTGAGAATGG GGCATGGGCG TACCTCAGCC CTCTGGTGCT GCGTAAAGAA 
CTAGAGTCCC TGGTAGAGAA TGAGGGCATC GAGGTGTTGG CGTTGCCTGA ACTGCCTGCT 
GCCCACCCTA TCATCTTCTG GAACCTCCTG TGGTATTTCC AACGGCTACG CCTGCCTAGT 
ATTCTGCCAG GCCTGGTGTT GGCCTCCTGT AATGGGCCGC CACCCAGCCA GCTCTCCCAG 
GGACCATCTT CATGGCTCAC CCCTGATCCA GCTTCTGTGC ATGTGCGTCT GCTGTGGGAC 
GTCCTGATCC CTGACCCCAA TAGCTGCCCG CCTCTCTACG TGCTCTGGAG GGTCCACAGC 
CAGATCCCAC AGCGGGTGGT GTGGCCAGGT CCAGTACCCT CCTGTCTTAG CTTGGCATTA 
CTGGAGTCAG TGCTGCGCCA TTTCGGACTC AACGAGGTTC ATAAAGCTGT TGGACTTCTG 
TTGGAGACTC TAGGGCCCCC TCCCACTGGC CTGCACCTGC AGAGGGGAAT CTACCGTGAG 
ATCTTATTCC TGACGATGGC TGCTCTGGGC AAGGACCACG TGGATATAGT GGCCTTCGAC 
AAGAAGTACA AGTCTGCCTT TAACAAGCTG GCCAGCAGCA TGGGCAAGGA AGAGCTGAGG 
CAGCGGCGGG CACAGATGCC TTCCCCCAAA GCCATCGACT GCCGAAAGTG TTTTGGAGCA 
CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759471.1
CDS	528..5042
Translation	MAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYDGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGESTPHTYCRLPRNLNPGMWGPAVFLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVENLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSTRHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRVAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYHVLREMESRKVVLPDEVCYRVLMQLCSNYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVWGAAQFRQPLKDRRRLQQEQQEQQQQQQQQQQQVAEQQKSGSSQTEPCLERPSPTRPLQRQTTWAGRSLREPSSPIGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEELAPGSSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEAGTPRKGLGARLQQLLTPSRRSSASRISVPELPPDLPPAARRSPMDSLLWPRERPGSTASEVSSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKSALAGAGGCKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGIEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPGLVLASCNGPPPSQLSQGPSSWLTPDPASVHVRLLWDVLIPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHFGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPSPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_008761249.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DENN domain containing 4B (Dennd4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761249.2

ORF Insert Sequence:

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTCG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCAC CTATACCTGA GGAGACGTGG GTTCCTGAGC CTACTGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT CACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAAGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCTGGTG GTCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTTGTTG AGCTGGGGGT ATTATATGAC GGGAAAGAAC GACCTAAGCC TGGGTTCCAA 
GTGCTTGACA CGACGCCATA TAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCATGTA CCGGCGTGCA GCAGAAGGGG CAGGTCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGA GCACTCCTCA TACTTACTGC 
CGGTTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTTCCT GTGCTACAAG 
GTGGGCCTGG CGAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTAGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCT GAGTCAGTGC CAGTCTTCTG CCTGCCCATG 
GGAGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCCGT CTTTTCCACC 
TTTGTGCTCA CGGGAGCTGC TGGTGATAAG GTGTATGGTG CCGCGTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT CCTGAGTGCC 
GTGGAACGGG GACGGGCCCT GGGAGGCCGA GCAGTGCGCA GTCGGAGAGC CATTGCTGTG 
CTGTCCCGCT GGCCCGCCTT CCCGGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGCTAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCGTTCC CTTCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTGA GTGGTGCTAG CTTTTTACAG 
CTGCTGCAGA ACCTGGGCCC AGAATTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC TCTGCGGCCA GATCTGCTTA CCAGTGTCTG CGAGGCCCTT 
GTCTCAATGA TTTTCCCTCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCTGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGACACCAA CACGCTCTTT 
CAAACGGAGG AAAAGAAGCC CCTCTCTGCT AGGACCCTGC CCCGAAGACC TTACAAGCTT 
CTGCTGGCCA CACTGACGAA TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CAGTGTGTGG CCGCAGGACT 
CGCCTAGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGC 
TACCGGAACT TCCTTCGACC TCTCACCCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAG 
AACCTTTTCT ACCTTCAGGG TTTCCTTAAG TCCCGGGAAC GCTCCAGTCA CAAGCTGTAC 
TCTCAGCTCC TACACACGCA GATGTTCTCC CAGTTCATCG AGGAATGCTC TTTCGGCTCT 
ACCCGGCATG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCTG AGCCAACACC CTTAGTGGAG CTGGAGGAAC TATCGGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCGCCA GTACTGGAAG GCAGTGAATC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTGAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCAC TCCCTGTGCC AGGCCCATCC CGTAGTGCCC CCAGCAGTCC TGCACCTCGC 
CGTACCAAAC AGGAGATGAA GGTTGCACAG CGCGTGGCAC AGAAGTCAGC CACTGTACCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTATGGAC TGTGGTTCTT ATGCCTACCT 
GCCTATGTGC GTTCTGCACC TTCCCGGGTG CGAGCATTAC ACACAGCCTA CCATGTATTA 
CGTGAGATGG AGAGCCGCAA GGTGGTGCTC CCTGATGAGG TCTGTTACCG GGTACTGATG 
CAGCTCTGCT CAAACTATGG GCAACCCGTG TTGTCTGTGC GAGTCATGCT GGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACC TATGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCTGGGGGG CGGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCGGCT GCAGCAGGAG 
CAGCAGGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAAC AGGTGGCCGA GCAGCAAAAG 
TCGGGCAGCT CTCAGACAGA GCCCTGTCTG GAGCGTCCTT CCCCAACCCG CCCTCTTCAG 
CGCCAGACTA CTTGGGCTGG ACGCAGTCTA CGGGAGCCAT CTTCACCCAT CGGGCGCCTG 
GTCAAGAGCG GCAGCCTAGG CAGCGCCCGC GGGGCACAGC CCACTGTGGA GGCTGGCGTG 
GCTCACATGA TAGAGGCCTT GGGGGTCCTT GAACCCCGTG GATCACCTGT GCCTTGGCAG 
GATGGAAGTC TCTCAGACCT GAGTCTGACA GGGGAAGAAC TGGCACCTGG AAGCAGCCCA 
GGGGGCTCAG GCTCAGCCCT GAGTGCCCAG TCCACTGAGG CCCTAGAAGG GATCAGTGGG 
CGGGGTTCCA AAACTAGTGG ATGTCAGGAG GAGGCGGGCA CCCCCAGAAA AGGGCTGGGT 
GCCCGCCTCC AACAGCTGCT TACCCCTTCC CGCCGCTCCT CTGCTTCCCG GATCTCCGTA 
CCTGAGTTGC CCCCTGACCT GCCTCCTGCG GCTCGCCGGA GTCCCATGGA TAGCCTTCTG 
TGGCCCCGAG AACGCCCTGG ATCCACTGCT TCTGAGGTAA GTTCTGCTTC TCTGGGCAGT 
GAGTGGGATA TCTCAGAGTC TTCTCTCAGC AGCCTGAGTC TTCGACGTTC CTCAGAGCGT 
CTCAGCGACA CCCCTGGAGC TTTCCAACCA CCCTCCCTAG AAATTCTGAT GTCTAGCTGC 
TCCCTGTGCC ATGCCTGTGA TTCCCTGGTG TATGATGAGG AAATCATGGC CGGCTGGGCA 
CCGGATGACT CTAACCTCAA TACGACTTGT CCCTTCTGCG CCTGTCCCTT CGTGCCCCTG 
CTTAGTGTCC AGACTCTTGA TTCTCGACCC AGTGCGCCTA GTCCAAAGTC TGCCCTTGCT 
GGTGCCGGTG GCTGCAAAGA TGCTCCTGTC CCTGGGGGTC CTGGCCCTGT GCTCAGTGAC 
CGGAGGCTGT GCCTTGCCCT GGATGAGCCC CAGCTCTGCA ATGGGCATAT GGGGAGTGCT 
TCCCGGCGAG TTGAGAATGG GGCATGGGCG TACCTCAGCC CTCTGGTGCT GCGTAAAGAA 
CTAGAGTCCC TGGTAGAGAA TGAGGGCATC GAGGTGTTGG CGTTGCCTGA ACTGCCTGCT 
GCCCACCCTA TCATCTTCTG GAACCTCCTG TGGTATTTCC AACGGCTACG CCTGCCTAGT 
ATTCTGCCAG GCCTGGTGTT GGCCTCCTGT AATGGGCCGC CACCCAGCCA GCTCTCCCAG 
GGACCATCTT CATGGCTCAC CCCTGATCCA GCTTCTGTGC ATGTGCGTCT GCTGTGGGAC 
GTCCTGATCC CTGACCCCAA TAGCTGCCCG CCTCTCTACG TGCTCTGGAG GGTCCACAGC 
CAGATCCCAC AGCGGGTGGT GTGGCCAGGT CCAGTACCCT CCTGTCTTAG CTTGGCATTA 
CTGGAGTCAG TGCTGCGCCA TTTCGGACTC AACGAGGTTC ATAAAGCTGT TGGACTTCTG 
TTGGAGACTC TAGGGCCCCC TCCCACTGGC CTGCACCTGC AGAGGGGAAT CTACCGTGAG 
ATCTTATTCC TGACGATGGC TGCTCTGGGC AAGGACCACG TGGATATAGT GGCCTTCGAC 
AAGAAGTACA AGTCTGCCTT TAACAAGCTG GCCAGCAGCA TGGGCAAGGA AGAGCTGAGG 
CAGCGGCGGG CACAGATGCC TTCCCCCAAA GCCATCGACT GCCGAAAGTG TTTTGGAGCA 
CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Dennd4b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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