Dennd4b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dennd4b
Entrez Gene ID	229541
Full Name	DENN/MADD domain containing 4B
General protein information	Preferred Names DENN/MADD domain containing 4B Names DENN domain-containing protein 4B brain specific protein 4 brain-specific gene 4 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_201407.4	NP_958809.3	DENN domain-containing protein 4B isoform 1
NM_001356483.1	NP_001343412.1	DENN domain-containing protein 4B isoform 2
XM_006501355.4	XP_006501418.1	DENN domain-containing protein 4B isoform X2
XM_006501356.3	XP_006501419.1	DENN domain-containing protein 4B isoform X2
XM_006501355.3	XP_006501418.1	DENN domain-containing protein 4B isoform X2
XM_006501357.1	XP_006501420.1	DENN domain-containing protein 4B isoform X3
XM_017319543.1	XP_017175032.1	DENN domain-containing protein 4B isoform X1
XM_017319543.2	XP_017175032.1	DENN domain-containing protein 4B isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DENND4B	XP_002686064.2
Danio rerio (zebrafish)	LOC798528	XP_002665116.3
Homo sapiens (human)	DENND4B	NP_055671.2
Pan troglodytes (chimpanzee)	DENND4B	XP_513830.3
Mus musculus (house mouse)	Dennd4b	NP_958809.3
Rattus norvegicus (Norway rat)	Dennd4b	XP_342288.5
Macaca mulatta (Rhesus monkey)	LOC718490	XP_002808284.1
Canis lupus familiaris (dog)	DENND4B	XP_537262.4

Gene Ontology
Bibliography

Related articles in PubMed

DENN domain proteins: regulators of Rab GTPases.
Marat AL, Dokainish H, McPherson PS
The Journal of biological chemistry286(16)13791-800(2011 Apr)

Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors.
Yoshimura S, Gerondopoulos A, Linford A, Rigden DJ, Barr FA
The Journal of cell biology191(2)367-81(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dennd4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dennd4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31830	NM_001356483.1 Latest version!	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu31830	XM_006501356.3 Latest version!	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu31830	XM_006501355.4 Latest version!	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu31831	XM_006501357.1 Latest version!	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu23536	NM_201407.4 Latest version!	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu80598	XM_017319543.2 Latest version!	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OMu31830	XM_006501355.3	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu80598	XM_017319543.1	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dennd4b ( NM_001356483.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343412.1 Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant 2, mRNA.

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CloneID	OMu31830
Clone ID Related Accession (Same CDS sequence)	NM_001356483.1 , XM_006501355.4 , XM_006501356.3 , XM_006501355.3
Accession Version	NM_001356483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571846400000
Organism	Mus musculus(house mouse)
Product	DENN domain-containing protein 4B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119825.11. On Oct 5, 2017 this sequence version replaced XM_006501356.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.145287.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTTG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCGC CTATACCTGA GGAGAAGTGG GTTCCCGAGC CTACCGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT TACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCGGGTG GCCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTATATGAA GGGAAGGAAC GACCCAAGCT TGGGTTCCAA 
GTGCTTGACA CGACACCGTA TAGCCACTCA GCCAATCTGG CCCCTCCAGG CCCCGGGCAC 
CCCCGCACCT ACCTCATGTA TCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGG GTACTCCTCA TACTTACTGC 
CGGCTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTAGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCC GAGTCAGTCC CTGTCTTCTG TCTGCCCATG 
GGGGCCACTA TTGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCTGT CTTCTCCACC 
TTTGTGCTCA CAGGCGCTGC TGGTGATAAG GTGTATGGTG CTGCTTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT GATGAGTGCC 
GTGGAACGGG GCCGGGCGCT GGGAGGCCGA GCTGTGCGCA GCCGACGAGC CATAGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGATAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCATTCC CATCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTCA GTGGTGCTAG CTTTTTGCAG 
CTGCTGCAGA ACCTGGGCCC AGAACTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTGTG CGAGGCCCTT 
GTCTCAATGA TCTTCCCTCT GCATTGGCAG TGTCCCTACA TCCCATTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGATACCAA CACGCTCTTC 
CAAAAGGAGG AAAAGAAGCC CCTCTCCGCT AGGACCCTGC CCCGAAGACC ATACAAGCTT 
TTATTGGCCA CCCTGACAAG TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CTGTGTGTGG CCGCAGGACT 
CGCCTGGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGT 
TACCGGAACT TCCTCCGTCC TCTCACGCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAC 
AACCTTTTTT ACCTTCAGGG TTTCCTCAAG TCCCGGGAAC GCTCAAGCCA CAAGCTGTAC 
TCTCAGCTGC TGCACACACA GATGTTCTCA CAGTTCATCG AGGAATGCTC TTTTGGCTCC 
GCCCGGCACG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCCG AACCAACACC CTTAGTGGAG CTGGAGGAAC TATCAGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCACCA GTACTGGAAG GCAGTGAGTC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTAAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCCC TCCCTGTGCC AGGCCCATCC CGGAGTGCTC CCAGCAGTCC TGCACCTCGC 
CGAACCAAAC AGGAGATGAA GGTTGCACAG CGAATGGCAC AGAAGTCAGC CACTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTACGGAC TGTGGTTCTT ATGCCTGCCC 
GCCTACGTGC GTTCCGTACC CTCCCGGGTG CGTGCATTAC ATACGGCCTA CCACGTGTTA 
CGGGAAATGG AGAACCGCAA GGTGGTGCTC CCTGATGAGG TGTGTTACCG CGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCCGTT CTGTCTGTGC GAGTCATGCT AGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACT TACGGTTACT ACAACAAGGC AGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GAAGCAACAG GTGGCCGAGC AGCAAAAGTC GGGCAGCTCT 
CAGACAGAGC CCTATCTGGA GCGTCCCTCC CCAACGCGCC CTCTTCAGCG CCAGACTACT 
TGGGCTGGGC GAAGTCTACG GGAACCATCT TCACCCATGG GGCGCCTGGT CAAGAGTGGT 
AGCCTAGGCA GTGCCCGTGG GACACAGCCC ACGGTGGAGG CTGGTGTGGC TCACATGATA 
GAGGCCTTGG GGGTCCTGGA ACCCCGCGGA TCACCTGTGC CTTGGCAGGA CGGAAGCCTC 
TCAGACCTGA GTCTGACAGG GGAAGAAATG GCACCTGGAG GCAGCCCTGG GGGCTCAGGC 
TCCGCCCTGA GTGCCCAGTC CACTGAGGCC CTAGAAGGGA TAAGTGGGCG GGGTTCCAAA 
ACTAGTGGAT GTCAGGAGGA GGTGGGCACT CCCAGAAAAG GGCTGGGTGC CCGCCTCCAA 
CAGCTGCTCA CCCCTTCCCG CCGCGCCTCT GCTTCCCGCA TCCCCCCACC TGAGTTGCCC 
TCTGACCTGC CTCCTGCGGC CCGCCGAAGT CCCATGGATA GCCTTCTGTG GCCCCGAGAA 
CGCCCCGGAT CCACTGCTTC TGAGAGCTCT GCTTCTCTGG GCAGTGAGTG GGATATCTCA 
GAGTCTTCCC TCAGCAGCCT GAGTCTCCGC CGTTCCTCAG AGCGTCTCAG TGACACCCCT 
GGGGCTTTCC AGCCACCCTC TCTGGAAATT CTGATGTCTA GCTGTTCCTT GTGTCATGCC 
TGTGATTCCC TGGTGTACGA TGAGGAAATC ATGGCTGGCT GGGCACCGGA TGACTCTAAC 
CTTAATACAA CTTGTCCCTT CTGCGCCTGT CACTTTGTGC CCCTGCTCAG TGTCCAGACT 
CTTGATTCTC GACCCAGTGC ACCTAGTCCA AAGTCTTCCC TTGCTGGCGC CAGTGGCTGC 
AAAGATGCTC CTGCCCCTGG GGGCCCTGGC CCTGTGCTCA GTGACCGGAG GTTCTGCCTT 
GCCCTGGATC AGCCCCAGCT CTGCAATGGG CATATGGGGA GTGCTTCCCG GCGAGTTGAG 
AATGGGGCAT GGGCGTACCT GAGCCCTCTG GTGCTGCGTA AGGAACTGGA GTCCCTGGTA 
GAGAATGAGG GCAGCGAGGT GCTGGCGTTA CCTGAACTGC CTGCTGCCCA CCCTATCATC 
TTCTGGAACC TCCTGTGGTA TTTCCAACGG CTACGCCTGC CTAGTGTTCT ACCAGGCCTG 
GTGTTGGCCT CCTGTAATGG GCCCCCACCC AGCCAGCTCT CCCAGGGACC ATCTCCATGG 
CTAACCCCTG ATCCAGCTTC TGTGCATGTG CATCTGCTGT GGGATGTCCT GACCCCTGAT 
CCCAACAGCT GCCCACCTCT CTACGTGCTC TGGAGGGTCC ACAGCCAGAT CCCACAGCGG 
GTGGTGTGGC CAGGCCCAGT ACCCTCATGT CTTAGCTTGG CATTACTGGA GTCAGTGCTG 
CGCCACGTCG GACTCAACGA GGTTCATAAA GCTGTTGGGC TTCTGCTGGA GACGCTAGGG 
CCCCCTCCCA CCGGCCTGCA CCTACAGAGG GGAATCTACC GTGAGATCTT ATTCCTGACC 
ATGGCTGCTC TGGGCAAGGA CCACGTGGAT ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAAC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CCAAGGCCAT CGACTGCCGA AAGTGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343412.1
CDS	351..4850
Translation	MAEERPPRLVDYFVVAGLAGNGAPIPEEKWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLMSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQKEEKKPLSARTLPRRPYKLLLATLTSLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSVPSRVRALHTAYHVLREMENRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKDRRQQQQQQQQQQQQKQQVAEQQKSGSSQTEPYLERPSPTRPLQRQTTWAGRSLREPSSPMGRLVKSGSLGSARGTQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEEMAPGGSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEVGTPRKGLGARLQQLLTPSRRASASRIPPPELPSDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACHFVPLLSVQTLDSRPSAPSPKSSLAGASGCKDAPAPGGPGPVLSDRRFCLALDQPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSVLPGLVLASCNGPPPSQLSQGPSPWLTPDPASVHVHLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	NM_001356483.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356483.1

ORF Insert Sequence:

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTTG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCGC CTATACCTGA GGAGAAGTGG GTTCCCGAGC CTACCGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT TACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCGGGTG GCCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTATATGAA GGGAAGGAAC GACCCAAGCT TGGGTTCCAA 
GTGCTTGACA CGACACCGTA TAGCCACTCA GCCAATCTGG CCCCTCCAGG CCCCGGGCAC 
CCCCGCACCT ACCTCATGTA TCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGG GTACTCCTCA TACTTACTGC 
CGGCTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTAGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCC GAGTCAGTCC CTGTCTTCTG TCTGCCCATG 
GGGGCCACTA TTGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCTGT CTTCTCCACC 
TTTGTGCTCA CAGGCGCTGC TGGTGATAAG GTGTATGGTG CTGCTTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT GATGAGTGCC 
GTGGAACGGG GCCGGGCGCT GGGAGGCCGA GCTGTGCGCA GCCGACGAGC CATAGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGATAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCATTCC CATCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTCA GTGGTGCTAG CTTTTTGCAG 
CTGCTGCAGA ACCTGGGCCC AGAACTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTGTG CGAGGCCCTT 
GTCTCAATGA TCTTCCCTCT GCATTGGCAG TGTCCCTACA TCCCATTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGATACCAA CACGCTCTTC 
CAAAAGGAGG AAAAGAAGCC CCTCTCCGCT AGGACCCTGC CCCGAAGACC ATACAAGCTT 
TTATTGGCCA CCCTGACAAG TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CTGTGTGTGG CCGCAGGACT 
CGCCTGGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGT 
TACCGGAACT TCCTCCGTCC TCTCACGCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAC 
AACCTTTTTT ACCTTCAGGG TTTCCTCAAG TCCCGGGAAC GCTCAAGCCA CAAGCTGTAC 
TCTCAGCTGC TGCACACACA GATGTTCTCA CAGTTCATCG AGGAATGCTC TTTTGGCTCC 
GCCCGGCACG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCCG AACCAACACC CTTAGTGGAG CTGGAGGAAC TATCAGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCACCA GTACTGGAAG GCAGTGAGTC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTAAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCCC TCCCTGTGCC AGGCCCATCC CGGAGTGCTC CCAGCAGTCC TGCACCTCGC 
CGAACCAAAC AGGAGATGAA GGTTGCACAG CGAATGGCAC AGAAGTCAGC CACTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTACGGAC TGTGGTTCTT ATGCCTGCCC 
GCCTACGTGC GTTCCGTACC CTCCCGGGTG CGTGCATTAC ATACGGCCTA CCACGTGTTA 
CGGGAAATGG AGAACCGCAA GGTGGTGCTC CCTGATGAGG TGTGTTACCG CGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCCGTT CTGTCTGTGC GAGTCATGCT AGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACT TACGGTTACT ACAACAAGGC AGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GAAGCAACAG GTGGCCGAGC AGCAAAAGTC GGGCAGCTCT 
CAGACAGAGC CCTATCTGGA GCGTCCCTCC CCAACGCGCC CTCTTCAGCG CCAGACTACT 
TGGGCTGGGC GAAGTCTACG GGAACCATCT TCACCCATGG GGCGCCTGGT CAAGAGTGGT 
AGCCTAGGCA GTGCCCGTGG GACACAGCCC ACGGTGGAGG CTGGTGTGGC TCACATGATA 
GAGGCCTTGG GGGTCCTGGA ACCCCGCGGA TCACCTGTGC CTTGGCAGGA CGGAAGCCTC 
TCAGACCTGA GTCTGACAGG GGAAGAAATG GCACCTGGAG GCAGCCCTGG GGGCTCAGGC 
TCCGCCCTGA GTGCCCAGTC CACTGAGGCC CTAGAAGGGA TAAGTGGGCG GGGTTCCAAA 
ACTAGTGGAT GTCAGGAGGA GGTGGGCACT CCCAGAAAAG GGCTGGGTGC CCGCCTCCAA 
CAGCTGCTCA CCCCTTCCCG CCGCGCCTCT GCTTCCCGCA TCCCCCCACC TGAGTTGCCC 
TCTGACCTGC CTCCTGCGGC CCGCCGAAGT CCCATGGATA GCCTTCTGTG GCCCCGAGAA 
CGCCCCGGAT CCACTGCTTC TGAGAGCTCT GCTTCTCTGG GCAGTGAGTG GGATATCTCA 
GAGTCTTCCC TCAGCAGCCT GAGTCTCCGC CGTTCCTCAG AGCGTCTCAG TGACACCCCT 
GGGGCTTTCC AGCCACCCTC TCTGGAAATT CTGATGTCTA GCTGTTCCTT GTGTCATGCC 
TGTGATTCCC TGGTGTACGA TGAGGAAATC ATGGCTGGCT GGGCACCGGA TGACTCTAAC 
CTTAATACAA CTTGTCCCTT CTGCGCCTGT CACTTTGTGC CCCTGCTCAG TGTCCAGACT 
CTTGATTCTC GACCCAGTGC ACCTAGTCCA AAGTCTTCCC TTGCTGGCGC CAGTGGCTGC 
AAAGATGCTC CTGCCCCTGG GGGCCCTGGC CCTGTGCTCA GTGACCGGAG GTTCTGCCTT 
GCCCTGGATC AGCCCCAGCT CTGCAATGGG CATATGGGGA GTGCTTCCCG GCGAGTTGAG 
AATGGGGCAT GGGCGTACCT GAGCCCTCTG GTGCTGCGTA AGGAACTGGA GTCCCTGGTA 
GAGAATGAGG GCAGCGAGGT GCTGGCGTTA CCTGAACTGC CTGCTGCCCA CCCTATCATC 
TTCTGGAACC TCCTGTGGTA TTTCCAACGG CTACGCCTGC CTAGTGTTCT ACCAGGCCTG 
GTGTTGGCCT CCTGTAATGG GCCCCCACCC AGCCAGCTCT CCCAGGGACC ATCTCCATGG 
CTAACCCCTG ATCCAGCTTC TGTGCATGTG CATCTGCTGT GGGATGTCCT GACCCCTGAT 
CCCAACAGCT GCCCACCTCT CTACGTGCTC TGGAGGGTCC ACAGCCAGAT CCCACAGCGG 
GTGGTGTGGC CAGGCCCAGT ACCCTCATGT CTTAGCTTGG CATTACTGGA GTCAGTGCTG 
CGCCACGTCG GACTCAACGA GGTTCATAAA GCTGTTGGGC TTCTGCTGGA GACGCTAGGG 
CCCCCTCCCA CCGGCCTGCA CCTACAGAGG GGAATCTACC GTGAGATCTT ATTCCTGACC 
ATGGCTGCTC TGGGCAAGGA CCACGTGGAT ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAAC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CCAAGGCCAT CGACTGCCGA AAGTGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_006501355.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501418.1 Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31830
Clone ID Related Accession (Same CDS sequence)	NM_001356483.1 , XM_006501355.4 , XM_006501356.3 , XM_006501355.3
Accession Version	XM_006501355.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501355.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTTG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCGC CTATACCTGA GGAGAAGTGG GTTCCCGAGC CTACCGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT TACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCGGGTG GCCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTATATGAA GGGAAGGAAC GACCCAAGCT TGGGTTCCAA 
GTGCTTGACA CGACACCGTA TAGCCACTCA GCCAATCTGG CCCCTCCAGG CCCCGGGCAC 
CCCCGCACCT ACCTCATGTA TCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGG GTACTCCTCA TACTTACTGC 
CGGCTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTAGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCC GAGTCAGTCC CTGTCTTCTG TCTGCCCATG 
GGGGCCACTA TTGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCTGT CTTCTCCACC 
TTTGTGCTCA CAGGCGCTGC TGGTGATAAG GTGTATGGTG CTGCTTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT GATGAGTGCC 
GTGGAACGGG GCCGGGCGCT GGGAGGCCGA GCTGTGCGCA GCCGACGAGC CATAGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGATAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCATTCC CATCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTCA GTGGTGCTAG CTTTTTGCAG 
CTGCTGCAGA ACCTGGGCCC AGAACTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTGTG CGAGGCCCTT 
GTCTCAATGA TCTTCCCTCT GCATTGGCAG TGTCCCTACA TCCCATTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGATACCAA CACGCTCTTC 
CAAAAGGAGG AAAAGAAGCC CCTCTCCGCT AGGACCCTGC CCCGAAGACC ATACAAGCTT 
TTATTGGCCA CCCTGACAAG TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CTGTGTGTGG CCGCAGGACT 
CGCCTGGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGT 
TACCGGAACT TCCTCCGTCC TCTCACGCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAC 
AACCTTTTTT ACCTTCAGGG TTTCCTCAAG TCCCGGGAAC GCTCAAGCCA CAAGCTGTAC 
TCTCAGCTGC TGCACACACA GATGTTCTCA CAGTTCATCG AGGAATGCTC TTTTGGCTCC 
GCCCGGCACG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCCG AACCAACACC CTTAGTGGAG CTGGAGGAAC TATCAGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCACCA GTACTGGAAG GCAGTGAGTC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTAAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCCC TCCCTGTGCC AGGCCCATCC CGGAGTGCTC CCAGCAGTCC TGCACCTCGC 
CGAACCAAAC AGGAGATGAA GGTTGCACAG CGAATGGCAC AGAAGTCAGC CACTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTACGGAC TGTGGTTCTT ATGCCTGCCC 
GCCTACGTGC GTTCCGTACC CTCCCGGGTG CGTGCATTAC ATACGGCCTA CCACGTGTTA 
CGGGAAATGG AGAACCGCAA GGTGGTGCTC CCTGATGAGG TGTGTTACCG CGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCCGTT CTGTCTGTGC GAGTCATGCT AGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACT TACGGTTACT ACAACAAGGC AGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GAAGCAACAG GTGGCCGAGC AGCAAAAGTC GGGCAGCTCT 
CAGACAGAGC CCTATCTGGA GCGTCCCTCC CCAACGCGCC CTCTTCAGCG CCAGACTACT 
TGGGCTGGGC GAAGTCTACG GGAACCATCT TCACCCATGG GGCGCCTGGT CAAGAGTGGT 
AGCCTAGGCA GTGCCCGTGG GACACAGCCC ACGGTGGAGG CTGGTGTGGC TCACATGATA 
GAGGCCTTGG GGGTCCTGGA ACCCCGCGGA TCACCTGTGC CTTGGCAGGA CGGAAGCCTC 
TCAGACCTGA GTCTGACAGG GGAAGAAATG GCACCTGGAG GCAGCCCTGG GGGCTCAGGC 
TCCGCCCTGA GTGCCCAGTC CACTGAGGCC CTAGAAGGGA TAAGTGGGCG GGGTTCCAAA 
ACTAGTGGAT GTCAGGAGGA GGTGGGCACT CCCAGAAAAG GGCTGGGTGC CCGCCTCCAA 
CAGCTGCTCA CCCCTTCCCG CCGCGCCTCT GCTTCCCGCA TCCCCCCACC TGAGTTGCCC 
TCTGACCTGC CTCCTGCGGC CCGCCGAAGT CCCATGGATA GCCTTCTGTG GCCCCGAGAA 
CGCCCCGGAT CCACTGCTTC TGAGAGCTCT GCTTCTCTGG GCAGTGAGTG GGATATCTCA 
GAGTCTTCCC TCAGCAGCCT GAGTCTCCGC CGTTCCTCAG AGCGTCTCAG TGACACCCCT 
GGGGCTTTCC AGCCACCCTC TCTGGAAATT CTGATGTCTA GCTGTTCCTT GTGTCATGCC 
TGTGATTCCC TGGTGTACGA TGAGGAAATC ATGGCTGGCT GGGCACCGGA TGACTCTAAC 
CTTAATACAA CTTGTCCCTT CTGCGCCTGT CACTTTGTGC CCCTGCTCAG TGTCCAGACT 
CTTGATTCTC GACCCAGTGC ACCTAGTCCA AAGTCTTCCC TTGCTGGCGC CAGTGGCTGC 
AAAGATGCTC CTGCCCCTGG GGGCCCTGGC CCTGTGCTCA GTGACCGGAG GTTCTGCCTT 
GCCCTGGATC AGCCCCAGCT CTGCAATGGG CATATGGGGA GTGCTTCCCG GCGAGTTGAG 
AATGGGGCAT GGGCGTACCT GAGCCCTCTG GTGCTGCGTA AGGAACTGGA GTCCCTGGTA 
GAGAATGAGG GCAGCGAGGT GCTGGCGTTA CCTGAACTGC CTGCTGCCCA CCCTATCATC 
TTCTGGAACC TCCTGTGGTA TTTCCAACGG CTACGCCTGC CTAGTGTTCT ACCAGGCCTG 
GTGTTGGCCT CCTGTAATGG GCCCCCACCC AGCCAGCTCT CCCAGGGACC ATCTCCATGG 
CTAACCCCTG ATCCAGCTTC TGTGCATGTG CATCTGCTGT GGGATGTCCT GACCCCTGAT 
CCCAACAGCT GCCCACCTCT CTACGTGCTC TGGAGGGTCC ACAGCCAGAT CCCACAGCGG 
GTGGTGTGGC CAGGCCCAGT ACCCTCATGT CTTAGCTTGG CATTACTGGA GTCAGTGCTG 
CGCCACGTCG GACTCAACGA GGTTCATAAA GCTGTTGGGC TTCTGCTGGA GACGCTAGGG 
CCCCCTCCCA CCGGCCTGCA CCTACAGAGG GGAATCTACC GTGAGATCTT ATTCCTGACC 
ATGGCTGCTC TGGGCAAGGA CCACGTGGAT ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAAC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CCAAGGCCAT CGACTGCCGA AAGTGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501418.1
CDS	43..4542
Translation	MAEERPPRLVDYFVVAGLAGNGAPIPEEKWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLMSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQKEEKKPLSARTLPRRPYKLLLATLTSLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSVPSRVRALHTAYHVLREMENRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKDRRQQQQQQQQQQQQKQQVAEQQKSGSSQTEPYLERPSPTRPLQRQTTWAGRSLREPSSPMGRLVKSGSLGSARGTQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEEMAPGGSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEVGTPRKGLGARLQQLLTPSRRASASRIPPPELPSDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACHFVPLLSVQTLDSRPSAPSPKSSLAGASGCKDAPAPGGPGPVLSDRRFCLALDQPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSVLPGLVLASCNGPPPSQLSQGPSPWLTPDPASVHVHLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_006501355.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501355.4

ORF Insert Sequence:

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTTG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCGC CTATACCTGA GGAGAAGTGG GTTCCCGAGC CTACCGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT TACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCGGGTG GCCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTATATGAA GGGAAGGAAC GACCCAAGCT TGGGTTCCAA 
GTGCTTGACA CGACACCGTA TAGCCACTCA GCCAATCTGG CCCCTCCAGG CCCCGGGCAC 
CCCCGCACCT ACCTCATGTA TCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGG GTACTCCTCA TACTTACTGC 
CGGCTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTAGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCC GAGTCAGTCC CTGTCTTCTG TCTGCCCATG 
GGGGCCACTA TTGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCTGT CTTCTCCACC 
TTTGTGCTCA CAGGCGCTGC TGGTGATAAG GTGTATGGTG CTGCTTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT GATGAGTGCC 
GTGGAACGGG GCCGGGCGCT GGGAGGCCGA GCTGTGCGCA GCCGACGAGC CATAGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGATAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCATTCC CATCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTCA GTGGTGCTAG CTTTTTGCAG 
CTGCTGCAGA ACCTGGGCCC AGAACTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTGTG CGAGGCCCTT 
GTCTCAATGA TCTTCCCTCT GCATTGGCAG TGTCCCTACA TCCCATTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGATACCAA CACGCTCTTC 
CAAAAGGAGG AAAAGAAGCC CCTCTCCGCT AGGACCCTGC CCCGAAGACC ATACAAGCTT 
TTATTGGCCA CCCTGACAAG TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CTGTGTGTGG CCGCAGGACT 
CGCCTGGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGT 
TACCGGAACT TCCTCCGTCC TCTCACGCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAC 
AACCTTTTTT ACCTTCAGGG TTTCCTCAAG TCCCGGGAAC GCTCAAGCCA CAAGCTGTAC 
TCTCAGCTGC TGCACACACA GATGTTCTCA CAGTTCATCG AGGAATGCTC TTTTGGCTCC 
GCCCGGCACG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCCG AACCAACACC CTTAGTGGAG CTGGAGGAAC TATCAGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCACCA GTACTGGAAG GCAGTGAGTC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTAAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCCC TCCCTGTGCC AGGCCCATCC CGGAGTGCTC CCAGCAGTCC TGCACCTCGC 
CGAACCAAAC AGGAGATGAA GGTTGCACAG CGAATGGCAC AGAAGTCAGC CACTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTACGGAC TGTGGTTCTT ATGCCTGCCC 
GCCTACGTGC GTTCCGTACC CTCCCGGGTG CGTGCATTAC ATACGGCCTA CCACGTGTTA 
CGGGAAATGG AGAACCGCAA GGTGGTGCTC CCTGATGAGG TGTGTTACCG CGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCCGTT CTGTCTGTGC GAGTCATGCT AGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACT TACGGTTACT ACAACAAGGC AGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GAAGCAACAG GTGGCCGAGC AGCAAAAGTC GGGCAGCTCT 
CAGACAGAGC CCTATCTGGA GCGTCCCTCC CCAACGCGCC CTCTTCAGCG CCAGACTACT 
TGGGCTGGGC GAAGTCTACG GGAACCATCT TCACCCATGG GGCGCCTGGT CAAGAGTGGT 
AGCCTAGGCA GTGCCCGTGG GACACAGCCC ACGGTGGAGG CTGGTGTGGC TCACATGATA 
GAGGCCTTGG GGGTCCTGGA ACCCCGCGGA TCACCTGTGC CTTGGCAGGA CGGAAGCCTC 
TCAGACCTGA GTCTGACAGG GGAAGAAATG GCACCTGGAG GCAGCCCTGG GGGCTCAGGC 
TCCGCCCTGA GTGCCCAGTC CACTGAGGCC CTAGAAGGGA TAAGTGGGCG GGGTTCCAAA 
ACTAGTGGAT GTCAGGAGGA GGTGGGCACT CCCAGAAAAG GGCTGGGTGC CCGCCTCCAA 
CAGCTGCTCA CCCCTTCCCG CCGCGCCTCT GCTTCCCGCA TCCCCCCACC TGAGTTGCCC 
TCTGACCTGC CTCCTGCGGC CCGCCGAAGT CCCATGGATA GCCTTCTGTG GCCCCGAGAA 
CGCCCCGGAT CCACTGCTTC TGAGAGCTCT GCTTCTCTGG GCAGTGAGTG GGATATCTCA 
GAGTCTTCCC TCAGCAGCCT GAGTCTCCGC CGTTCCTCAG AGCGTCTCAG TGACACCCCT 
GGGGCTTTCC AGCCACCCTC TCTGGAAATT CTGATGTCTA GCTGTTCCTT GTGTCATGCC 
TGTGATTCCC TGGTGTACGA TGAGGAAATC ATGGCTGGCT GGGCACCGGA TGACTCTAAC 
CTTAATACAA CTTGTCCCTT CTGCGCCTGT CACTTTGTGC CCCTGCTCAG TGTCCAGACT 
CTTGATTCTC GACCCAGTGC ACCTAGTCCA AAGTCTTCCC TTGCTGGCGC CAGTGGCTGC 
AAAGATGCTC CTGCCCCTGG GGGCCCTGGC CCTGTGCTCA GTGACCGGAG GTTCTGCCTT 
GCCCTGGATC AGCCCCAGCT CTGCAATGGG CATATGGGGA GTGCTTCCCG GCGAGTTGAG 
AATGGGGCAT GGGCGTACCT GAGCCCTCTG GTGCTGCGTA AGGAACTGGA GTCCCTGGTA 
GAGAATGAGG GCAGCGAGGT GCTGGCGTTA CCTGAACTGC CTGCTGCCCA CCCTATCATC 
TTCTGGAACC TCCTGTGGTA TTTCCAACGG CTACGCCTGC CTAGTGTTCT ACCAGGCCTG 
GTGTTGGCCT CCTGTAATGG GCCCCCACCC AGCCAGCTCT CCCAGGGACC ATCTCCATGG 
CTAACCCCTG ATCCAGCTTC TGTGCATGTG CATCTGCTGT GGGATGTCCT GACCCCTGAT 
CCCAACAGCT GCCCACCTCT CTACGTGCTC TGGAGGGTCC ACAGCCAGAT CCCACAGCGG 
GTGGTGTGGC CAGGCCCAGT ACCCTCATGT CTTAGCTTGG CATTACTGGA GTCAGTGCTG 
CGCCACGTCG GACTCAACGA GGTTCATAAA GCTGTTGGGC TTCTGCTGGA GACGCTAGGG 
CCCCCTCCCA CCGGCCTGCA CCTACAGAGG GGAATCTACC GTGAGATCTT ATTCCTGACC 
ATGGCTGCTC TGGGCAAGGA CCACGTGGAT ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAAC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CCAAGGCCAT CGACTGCCGA AAGTGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_006501356.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501419.1 Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X3, mRNA.

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CloneID	OMu31830
Clone ID Related Accession (Same CDS sequence)	NM_001356483.1 , XM_006501355.4 , XM_006501356.3 , XM_006501355.3
Accession Version	XM_006501356.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(House mouse)
Product	DENN domain-containing protein 4B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTTG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCGC CTATACCTGA GGAGAAGTGG GTTCCCGAGC CTACCGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT TACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCGGGTG GCCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTATATGAA GGGAAGGAAC GACCCAAGCT TGGGTTCCAA 
GTGCTTGACA CGACACCGTA TAGCCACTCA GCCAATCTGG CCCCTCCAGG CCCCGGGCAC 
CCCCGCACCT ACCTCATGTA TCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGG GTACTCCTCA TACTTACTGC 
CGGCTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTAGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCC GAGTCAGTCC CTGTCTTCTG TCTGCCCATG 
GGGGCCACTA TTGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCTGT CTTCTCCACC 
TTTGTGCTCA CAGGCGCTGC TGGTGATAAG GTGTATGGTG CTGCTTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT GATGAGTGCC 
GTGGAACGGG GCCGGGCGCT GGGAGGCCGA GCTGTGCGCA GCCGACGAGC CATAGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGATAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCATTCC CATCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTCA GTGGTGCTAG CTTTTTGCAG 
CTGCTGCAGA ACCTGGGCCC AGAACTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTGTG CGAGGCCCTT 
GTCTCAATGA TCTTCCCTCT GCATTGGCAG TGTCCCTACA TCCCATTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGATACCAA CACGCTCTTC 
CAAAAGGAGG AAAAGAAGCC CCTCTCCGCT AGGACCCTGC CCCGAAGACC ATACAAGCTT 
TTATTGGCCA CCCTGACAAG TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CTGTGTGTGG CCGCAGGACT 
CGCCTGGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGT 
TACCGGAACT TCCTCCGTCC TCTCACGCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAC 
AACCTTTTTT ACCTTCAGGG TTTCCTCAAG TCCCGGGAAC GCTCAAGCCA CAAGCTGTAC 
TCTCAGCTGC TGCACACACA GATGTTCTCA CAGTTCATCG AGGAATGCTC TTTTGGCTCC 
GCCCGGCACG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCCG AACCAACACC CTTAGTGGAG CTGGAGGAAC TATCAGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCACCA GTACTGGAAG GCAGTGAGTC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTAAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCCC TCCCTGTGCC AGGCCCATCC CGGAGTGCTC CCAGCAGTCC TGCACCTCGC 
CGAACCAAAC AGGAGATGAA GGTTGCACAG CGAATGGCAC AGAAGTCAGC CACTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTACGGAC TGTGGTTCTT ATGCCTGCCC 
GCCTACGTGC GTTCCGTACC CTCCCGGGTG CGTGCATTAC ATACGGCCTA CCACGTGTTA 
CGGGAAATGG AGAACCGCAA GGTGGTGCTC CCTGATGAGG TGTGTTACCG CGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCCGTT CTGTCTGTGC GAGTCATGCT AGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACT TACGGTTACT ACAACAAGGC AGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GAAGCAACAG GTGGCCGAGC AGCAAAAGTC GGGCAGCTCT 
CAGACAGAGC CCTATCTGGA GCGTCCCTCC CCAACGCGCC CTCTTCAGCG CCAGACTACT 
TGGGCTGGGC GAAGTCTACG GGAACCATCT TCACCCATGG GGCGCCTGGT CAAGAGTGGT 
AGCCTAGGCA GTGCCCGTGG GACACAGCCC ACGGTGGAGG CTGGTGTGGC TCACATGATA 
GAGGCCTTGG GGGTCCTGGA ACCCCGCGGA TCACCTGTGC CTTGGCAGGA CGGAAGCCTC 
TCAGACCTGA GTCTGACAGG GGAAGAAATG GCACCTGGAG GCAGCCCTGG GGGCTCAGGC 
TCCGCCCTGA GTGCCCAGTC CACTGAGGCC CTAGAAGGGA TAAGTGGGCG GGGTTCCAAA 
ACTAGTGGAT GTCAGGAGGA GGTGGGCACT CCCAGAAAAG GGCTGGGTGC CCGCCTCCAA 
CAGCTGCTCA CCCCTTCCCG CCGCGCCTCT GCTTCCCGCA TCCCCCCACC TGAGTTGCCC 
TCTGACCTGC CTCCTGCGGC CCGCCGAAGT CCCATGGATA GCCTTCTGTG GCCCCGAGAA 
CGCCCCGGAT CCACTGCTTC TGAGAGCTCT GCTTCTCTGG GCAGTGAGTG GGATATCTCA 
GAGTCTTCCC TCAGCAGCCT GAGTCTCCGC CGTTCCTCAG AGCGTCTCAG TGACACCCCT 
GGGGCTTTCC AGCCACCCTC TCTGGAAATT CTGATGTCTA GCTGTTCCTT GTGTCATGCC 
TGTGATTCCC TGGTGTACGA TGAGGAAATC ATGGCTGGCT GGGCACCGGA TGACTCTAAC 
CTTAATACAA CTTGTCCCTT CTGCGCCTGT CACTTTGTGC CCCTGCTCAG TGTCCAGACT 
CTTGATTCTC GACCCAGTGC ACCTAGTCCA AAGTCTTCCC TTGCTGGCGC CAGTGGCTGC 
AAAGATGCTC CTGCCCCTGG GGGCCCTGGC CCTGTGCTCA GTGACCGGAG GTTCTGCCTT 
GCCCTGGATC AGCCCCAGCT CTGCAATGGG CATATGGGGA GTGCTTCCCG GCGAGTTGAG 
AATGGGGCAT GGGCGTACCT GAGCCCTCTG GTGCTGCGTA AGGAACTGGA GTCCCTGGTA 
GAGAATGAGG GCAGCGAGGT GCTGGCGTTA CCTGAACTGC CTGCTGCCCA CCCTATCATC 
TTCTGGAACC TCCTGTGGTA TTTCCAACGG CTACGCCTGC CTAGTGTTCT ACCAGGCCTG 
GTGTTGGCCT CCTGTAATGG GCCCCCACCC AGCCAGCTCT CCCAGGGACC ATCTCCATGG 
CTAACCCCTG ATCCAGCTTC TGTGCATGTG CATCTGCTGT GGGATGTCCT GACCCCTGAT 
CCCAACAGCT GCCCACCTCT CTACGTGCTC TGGAGGGTCC ACAGCCAGAT CCCACAGCGG 
GTGGTGTGGC CAGGCCCAGT ACCCTCATGT CTTAGCTTGG CATTACTGGA GTCAGTGCTG 
CGCCACGTCG GACTCAACGA GGTTCATAAA GCTGTTGGGC TTCTGCTGGA GACGCTAGGG 
CCCCCTCCCA CCGGCCTGCA CCTACAGAGG GGAATCTACC GTGAGATCTT ATTCCTGACC 
ATGGCTGCTC TGGGCAAGGA CCACGTGGAT ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAAC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CCAAGGCCAT CGACTGCCGA AAGTGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501419.1
CDS	395..4894
Translation	MAEERPPRLVDYFVVAGLAGNGAPIPEEKWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLMSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQKEEKKPLSARTLPRRPYKLLLATLTSLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSVPSRVRALHTAYHVLREMENRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKDRRQQQQQQQQQQQQKQQVAEQQKSGSSQTEPYLERPSPTRPLQRQTTWAGRSLREPSSPMGRLVKSGSLGSARGTQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEEMAPGGSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEVGTPRKGLGARLQQLLTPSRRASASRIPPPELPSDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACHFVPLLSVQTLDSRPSAPSPKSSLAGASGCKDAPAPGGPGPVLSDRRFCLALDQPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSVLPGLVLASCNGPPPSQLSQGPSPWLTPDPASVHVHLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_006501356.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501356.3

ORF Insert Sequence:

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTTG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCGC CTATACCTGA GGAGAAGTGG GTTCCCGAGC CTACCGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT TACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCGGGTG GCCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTATATGAA GGGAAGGAAC GACCCAAGCT TGGGTTCCAA 
GTGCTTGACA CGACACCGTA TAGCCACTCA GCCAATCTGG CCCCTCCAGG CCCCGGGCAC 
CCCCGCACCT ACCTCATGTA TCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGG GTACTCCTCA TACTTACTGC 
CGGCTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTAGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCC GAGTCAGTCC CTGTCTTCTG TCTGCCCATG 
GGGGCCACTA TTGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCTGT CTTCTCCACC 
TTTGTGCTCA CAGGCGCTGC TGGTGATAAG GTGTATGGTG CTGCTTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT GATGAGTGCC 
GTGGAACGGG GCCGGGCGCT GGGAGGCCGA GCTGTGCGCA GCCGACGAGC CATAGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGATAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCATTCC CATCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTCA GTGGTGCTAG CTTTTTGCAG 
CTGCTGCAGA ACCTGGGCCC AGAACTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTGTG CGAGGCCCTT 
GTCTCAATGA TCTTCCCTCT GCATTGGCAG TGTCCCTACA TCCCATTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGATACCAA CACGCTCTTC 
CAAAAGGAGG AAAAGAAGCC CCTCTCCGCT AGGACCCTGC CCCGAAGACC ATACAAGCTT 
TTATTGGCCA CCCTGACAAG TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CTGTGTGTGG CCGCAGGACT 
CGCCTGGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGT 
TACCGGAACT TCCTCCGTCC TCTCACGCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAC 
AACCTTTTTT ACCTTCAGGG TTTCCTCAAG TCCCGGGAAC GCTCAAGCCA CAAGCTGTAC 
TCTCAGCTGC TGCACACACA GATGTTCTCA CAGTTCATCG AGGAATGCTC TTTTGGCTCC 
GCCCGGCACG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCCG AACCAACACC CTTAGTGGAG CTGGAGGAAC TATCAGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCACCA GTACTGGAAG GCAGTGAGTC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTAAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCCC TCCCTGTGCC AGGCCCATCC CGGAGTGCTC CCAGCAGTCC TGCACCTCGC 
CGAACCAAAC AGGAGATGAA GGTTGCACAG CGAATGGCAC AGAAGTCAGC CACTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTACGGAC TGTGGTTCTT ATGCCTGCCC 
GCCTACGTGC GTTCCGTACC CTCCCGGGTG CGTGCATTAC ATACGGCCTA CCACGTGTTA 
CGGGAAATGG AGAACCGCAA GGTGGTGCTC CCTGATGAGG TGTGTTACCG CGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCCGTT CTGTCTGTGC GAGTCATGCT AGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACT TACGGTTACT ACAACAAGGC AGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GAAGCAACAG GTGGCCGAGC AGCAAAAGTC GGGCAGCTCT 
CAGACAGAGC CCTATCTGGA GCGTCCCTCC CCAACGCGCC CTCTTCAGCG CCAGACTACT 
TGGGCTGGGC GAAGTCTACG GGAACCATCT TCACCCATGG GGCGCCTGGT CAAGAGTGGT 
AGCCTAGGCA GTGCCCGTGG GACACAGCCC ACGGTGGAGG CTGGTGTGGC TCACATGATA 
GAGGCCTTGG GGGTCCTGGA ACCCCGCGGA TCACCTGTGC CTTGGCAGGA CGGAAGCCTC 
TCAGACCTGA GTCTGACAGG GGAAGAAATG GCACCTGGAG GCAGCCCTGG GGGCTCAGGC 
TCCGCCCTGA GTGCCCAGTC CACTGAGGCC CTAGAAGGGA TAAGTGGGCG GGGTTCCAAA 
ACTAGTGGAT GTCAGGAGGA GGTGGGCACT CCCAGAAAAG GGCTGGGTGC CCGCCTCCAA 
CAGCTGCTCA CCCCTTCCCG CCGCGCCTCT GCTTCCCGCA TCCCCCCACC TGAGTTGCCC 
TCTGACCTGC CTCCTGCGGC CCGCCGAAGT CCCATGGATA GCCTTCTGTG GCCCCGAGAA 
CGCCCCGGAT CCACTGCTTC TGAGAGCTCT GCTTCTCTGG GCAGTGAGTG GGATATCTCA 
GAGTCTTCCC TCAGCAGCCT GAGTCTCCGC CGTTCCTCAG AGCGTCTCAG TGACACCCCT 
GGGGCTTTCC AGCCACCCTC TCTGGAAATT CTGATGTCTA GCTGTTCCTT GTGTCATGCC 
TGTGATTCCC TGGTGTACGA TGAGGAAATC ATGGCTGGCT GGGCACCGGA TGACTCTAAC 
CTTAATACAA CTTGTCCCTT CTGCGCCTGT CACTTTGTGC CCCTGCTCAG TGTCCAGACT 
CTTGATTCTC GACCCAGTGC ACCTAGTCCA AAGTCTTCCC TTGCTGGCGC CAGTGGCTGC 
AAAGATGCTC CTGCCCCTGG GGGCCCTGGC CCTGTGCTCA GTGACCGGAG GTTCTGCCTT 
GCCCTGGATC AGCCCCAGCT CTGCAATGGG CATATGGGGA GTGCTTCCCG GCGAGTTGAG 
AATGGGGCAT GGGCGTACCT GAGCCCTCTG GTGCTGCGTA AGGAACTGGA GTCCCTGGTA 
GAGAATGAGG GCAGCGAGGT GCTGGCGTTA CCTGAACTGC CTGCTGCCCA CCCTATCATC 
TTCTGGAACC TCCTGTGGTA TTTCCAACGG CTACGCCTGC CTAGTGTTCT ACCAGGCCTG 
GTGTTGGCCT CCTGTAATGG GCCCCCACCC AGCCAGCTCT CCCAGGGACC ATCTCCATGG 
CTAACCCCTG ATCCAGCTTC TGTGCATGTG CATCTGCTGT GGGATGTCCT GACCCCTGAT 
CCCAACAGCT GCCCACCTCT CTACGTGCTC TGGAGGGTCC ACAGCCAGAT CCCACAGCGG 
GTGGTGTGGC CAGGCCCAGT ACCCTCATGT CTTAGCTTGG CATTACTGGA GTCAGTGCTG 
CGCCACGTCG GACTCAACGA GGTTCATAAA GCTGTTGGGC TTCTGCTGGA GACGCTAGGG 
CCCCCTCCCA CCGGCCTGCA CCTACAGAGG GGAATCTACC GTGAGATCTT ATTCCTGACC 
ATGGCTGCTC TGGGCAAGGA CCACGTGGAT ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAAC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CCAAGGCCAT CGACTGCCGA AAGTGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_006501355.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501418.1 Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X2, mRNA.

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CloneID	OMu31830
Clone ID Related Accession (Same CDS sequence)	NM_001356483.1 , XM_006501355.4 , XM_006501356.3 , XM_006501355.3
Accession Version	XM_006501355.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTTG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCGC CTATACCTGA GGAGAAGTGG GTTCCCGAGC CTACCGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT TACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCGGGTG GCCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTATATGAA GGGAAGGAAC GACCCAAGCT TGGGTTCCAA 
GTGCTTGACA CGACACCGTA TAGCCACTCA GCCAATCTGG CCCCTCCAGG CCCCGGGCAC 
CCCCGCACCT ACCTCATGTA TCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGG GTACTCCTCA TACTTACTGC 
CGGCTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTAGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCC GAGTCAGTCC CTGTCTTCTG TCTGCCCATG 
GGGGCCACTA TTGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCTGT CTTCTCCACC 
TTTGTGCTCA CAGGCGCTGC TGGTGATAAG GTGTATGGTG CTGCTTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT GATGAGTGCC 
GTGGAACGGG GCCGGGCGCT GGGAGGCCGA GCTGTGCGCA GCCGACGAGC CATAGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGATAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCATTCC CATCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTCA GTGGTGCTAG CTTTTTGCAG 
CTGCTGCAGA ACCTGGGCCC AGAACTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTGTG CGAGGCCCTT 
GTCTCAATGA TCTTCCCTCT GCATTGGCAG TGTCCCTACA TCCCATTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGATACCAA CACGCTCTTC 
CAAAAGGAGG AAAAGAAGCC CCTCTCCGCT AGGACCCTGC CCCGAAGACC ATACAAGCTT 
TTATTGGCCA CCCTGACAAG TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CTGTGTGTGG CCGCAGGACT 
CGCCTGGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGT 
TACCGGAACT TCCTCCGTCC TCTCACGCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAC 
AACCTTTTTT ACCTTCAGGG TTTCCTCAAG TCCCGGGAAC GCTCAAGCCA CAAGCTGTAC 
TCTCAGCTGC TGCACACACA GATGTTCTCA CAGTTCATCG AGGAATGCTC TTTTGGCTCC 
GCCCGGCACG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCCG AACCAACACC CTTAGTGGAG CTGGAGGAAC TATCAGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCACCA GTACTGGAAG GCAGTGAGTC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTAAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCCC TCCCTGTGCC AGGCCCATCC CGGAGTGCTC CCAGCAGTCC TGCACCTCGC 
CGAACCAAAC AGGAGATGAA GGTTGCACAG CGAATGGCAC AGAAGTCAGC CACTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTACGGAC TGTGGTTCTT ATGCCTGCCC 
GCCTACGTGC GTTCCGTACC CTCCCGGGTG CGTGCATTAC ATACGGCCTA CCACGTGTTA 
CGGGAAATGG AGAACCGCAA GGTGGTGCTC CCTGATGAGG TGTGTTACCG CGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCCGTT CTGTCTGTGC GAGTCATGCT AGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACT TACGGTTACT ACAACAAGGC AGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GAAGCAACAG GTGGCCGAGC AGCAAAAGTC GGGCAGCTCT 
CAGACAGAGC CCTATCTGGA GCGTCCCTCC CCAACGCGCC CTCTTCAGCG CCAGACTACT 
TGGGCTGGGC GAAGTCTACG GGAACCATCT TCACCCATGG GGCGCCTGGT CAAGAGTGGT 
AGCCTAGGCA GTGCCCGTGG GACACAGCCC ACGGTGGAGG CTGGTGTGGC TCACATGATA 
GAGGCCTTGG GGGTCCTGGA ACCCCGCGGA TCACCTGTGC CTTGGCAGGA CGGAAGCCTC 
TCAGACCTGA GTCTGACAGG GGAAGAAATG GCACCTGGAG GCAGCCCTGG GGGCTCAGGC 
TCCGCCCTGA GTGCCCAGTC CACTGAGGCC CTAGAAGGGA TAAGTGGGCG GGGTTCCAAA 
ACTAGTGGAT GTCAGGAGGA GGTGGGCACT CCCAGAAAAG GGCTGGGTGC CCGCCTCCAA 
CAGCTGCTCA CCCCTTCCCG CCGCGCCTCT GCTTCCCGCA TCCCCCCACC TGAGTTGCCC 
TCTGACCTGC CTCCTGCGGC CCGCCGAAGT CCCATGGATA GCCTTCTGTG GCCCCGAGAA 
CGCCCCGGAT CCACTGCTTC TGAGAGCTCT GCTTCTCTGG GCAGTGAGTG GGATATCTCA 
GAGTCTTCCC TCAGCAGCCT GAGTCTCCGC CGTTCCTCAG AGCGTCTCAG TGACACCCCT 
GGGGCTTTCC AGCCACCCTC TCTGGAAATT CTGATGTCTA GCTGTTCCTT GTGTCATGCC 
TGTGATTCCC TGGTGTACGA TGAGGAAATC ATGGCTGGCT GGGCACCGGA TGACTCTAAC 
CTTAATACAA CTTGTCCCTT CTGCGCCTGT CACTTTGTGC CCCTGCTCAG TGTCCAGACT 
CTTGATTCTC GACCCAGTGC ACCTAGTCCA AAGTCTTCCC TTGCTGGCGC CAGTGGCTGC 
AAAGATGCTC CTGCCCCTGG GGGCCCTGGC CCTGTGCTCA GTGACCGGAG GTTCTGCCTT 
GCCCTGGATC AGCCCCAGCT CTGCAATGGG CATATGGGGA GTGCTTCCCG GCGAGTTGAG 
AATGGGGCAT GGGCGTACCT GAGCCCTCTG GTGCTGCGTA AGGAACTGGA GTCCCTGGTA 
GAGAATGAGG GCAGCGAGGT GCTGGCGTTA CCTGAACTGC CTGCTGCCCA CCCTATCATC 
TTCTGGAACC TCCTGTGGTA TTTCCAACGG CTACGCCTGC CTAGTGTTCT ACCAGGCCTG 
GTGTTGGCCT CCTGTAATGG GCCCCCACCC AGCCAGCTCT CCCAGGGACC ATCTCCATGG 
CTAACCCCTG ATCCAGCTTC TGTGCATGTG CATCTGCTGT GGGATGTCCT GACCCCTGAT 
CCCAACAGCT GCCCACCTCT CTACGTGCTC TGGAGGGTCC ACAGCCAGAT CCCACAGCGG 
GTGGTGTGGC CAGGCCCAGT ACCCTCATGT CTTAGCTTGG CATTACTGGA GTCAGTGCTG 
CGCCACGTCG GACTCAACGA GGTTCATAAA GCTGTTGGGC TTCTGCTGGA GACGCTAGGG 
CCCCCTCCCA CCGGCCTGCA CCTACAGAGG GGAATCTACC GTGAGATCTT ATTCCTGACC 
ATGGCTGCTC TGGGCAAGGA CCACGTGGAT ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAAC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CCAAGGCCAT CGACTGCCGA AAGTGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501418.1
CDS	140..4639
Translation	MAEERPPRLVDYFVVAGLAGNGAPIPEEKWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLMSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQKEEKKPLSARTLPRRPYKLLLATLTSLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSVPSRVRALHTAYHVLREMENRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKDRRQQQQQQQQQQQQKQQVAEQQKSGSSQTEPYLERPSPTRPLQRQTTWAGRSLREPSSPMGRLVKSGSLGSARGTQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEEMAPGGSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEVGTPRKGLGARLQQLLTPSRRASASRIPPPELPSDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACHFVPLLSVQTLDSRPSAPSPKSSLAGASGCKDAPAPGGPGPVLSDRRFCLALDQPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSVLPGLVLASCNGPPPSQLSQGPSPWLTPDPASVHVHLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_006501355.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501355.3

ORF Insert Sequence:

ATGGCAGAGG AGCGGCCTCC CCGGTTGGTG GATTACTTTG TGGTGGCTGG GCTTGCAGGA 
AATGGAGCGC CTATACCTGA GGAGAAGTGG GTTCCCGAGC CTACCGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAACCTAT TACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATTCAGACT TCTGCGGGTG GCCACCCCTT GGAGCTCAGT 
GCTGGGCTCC TGGGTGGAAC TCAACCAGTC ATCTGCTACC GCAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTATATGAA GGGAAGGAAC GACCCAAGCT TGGGTTCCAA 
GTGCTTGACA CGACACCGTA TAGCCACTCA GCCAATCTGG CCCCTCCAGG CCCCGGGCAC 
CCCCGCACCT ACCTCATGTA TCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT TCTGCCCAGC AAGGGTGAGG GTACTCCTCA TACTTACTGC 
CGGCTACCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTAGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAAGACA ATGAGGCGTT TCCGCTGCCC GAGTCAGTCC CTGTCTTCTG TCTGCCCATG 
GGGGCCACTA TTGAGTGCTG GCCTGCCCAG ACCAAGTACC CAGTACCTGT CTTCTCCACC 
TTTGTGCTCA CAGGCGCTGC TGGTGATAAG GTGTATGGTG CTGCTTTGCA GTTCTATGAA 
GCGTTCCCGA GGGCCAGGCT GTCAGAGAGG CAGGCACGGG CACTGGGCCT GATGAGTGCC 
GTGGAACGGG GCCGGGCGCT GGGAGGCCGA GCTGTGCGCA GCCGACGAGC CATAGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGAGCCTTCC TCACCTTCCT TTACCGATAC 
TCCGTCTCAG GCCCCCATCG TCTGCCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCATTCC CATCCCCACA GAGACCACGC ATCCTAGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GTCAACCTGT CTCCTCACCC CTGCCCCTCA GTGGTGCTAG CTTTTTGCAG 
CTGCTGCAGA ACCTGGGCCC AGAACTGGCT ATTACATTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TGGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTGTG CGAGGCCCTT 
GTCTCAATGA TCTTCCCTCT GCATTGGCAG TGTCCCTACA TCCCATTGTG CCCGCTAGTG 
CTGGCGGATG TGCTGAGTGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGTTATTTC 
GATCTGCATG ACCCACCTGC TGACGTCATC TGTGTTGATC TTGATACCAA CACGCTCTTC 
CAAAAGGAGG AAAAGAAGCC CCTCTCCGCT AGGACCCTGC CCCGAAGACC ATACAAGCTT 
TTATTGGCCA CCCTGACAAG TCTGTACCAG CAGCTGGACC AGACTTACAC TGGACCAGAG 
GAAGAGGCCT CCCTGGAATT CCTGCTGACA GACTATGAGG CTGTGTGTGG CCGCAGGACT 
CGCCTGGAAC GGGAAGTCCA GGGAGCCTTC CTGCGCTTCA TGGCCTGTCT GCTCAAAGGT 
TACCGGAACT TCCTCCGTCC TCTCACGCAG GCCCCCTCTG AGGGGTCTCG AGATGTTGAC 
AACCTTTTTT ACCTTCAGGG TTTCCTCAAG TCCCGGGAAC GCTCAAGCCA CAAGCTGTAC 
TCTCAGCTGC TGCACACACA GATGTTCTCA CAGTTCATCG AGGAATGCTC TTTTGGCTCC 
GCCCGGCACG CAGCCCTGGA ATTCTTTGAC TCTTGTGTTG ACAAGGTTCA CCCAGAGCAG 
GAGAAGCCCG AACCAACACC CTTAGTGGAG CTGGAGGAAC TATCAGGAAG TGAGCTCACT 
GTCTTTATCA CACCCCCAGA GGAACCACCA GTACTGGAAG GCAGTGAGTC TACTCCTCAG 
TACTGTTATG ATGGGTTTCC AGAGCTAAAG GCTGAGCTAT TTGAGTCTCC TCAGGAACAA 
CAGGGGGCCC TCCCTGTGCC AGGCCCATCC CGGAGTGCTC CCAGCAGTCC TGCACCTCGC 
CGAACCAAAC AGGAGATGAA GGTTGCACAG CGAATGGCAC AGAAGTCAGC CACTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGTCAC TGCTACGGAC TGTGGTTCTT ATGCCTGCCC 
GCCTACGTGC GTTCCGTACC CTCCCGGGTG CGTGCATTAC ATACGGCCTA CCACGTGTTA 
CGGGAAATGG AGAACCGCAA GGTGGTGCTC CCTGATGAGG TGTGTTACCG CGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCCGTT CTGTCTGTGC GAGTCATGCT AGAGATGCGA 
CGGGCAGGCA TTGTGCCCAA CACCATCACT TACGGTTACT ACAACAAGGC AGTGCTGGAA 
AGCAAATGGC CATCTGGTAC TCCGGGTGGA CGCCTACGCT GGGCCAAGCT CCGGAATGTT 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTAAAGGACC GAAGGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GAAGCAACAG GTGGCCGAGC AGCAAAAGTC GGGCAGCTCT 
CAGACAGAGC CCTATCTGGA GCGTCCCTCC CCAACGCGCC CTCTTCAGCG CCAGACTACT 
TGGGCTGGGC GAAGTCTACG GGAACCATCT TCACCCATGG GGCGCCTGGT CAAGAGTGGT 
AGCCTAGGCA GTGCCCGTGG GACACAGCCC ACGGTGGAGG CTGGTGTGGC TCACATGATA 
GAGGCCTTGG GGGTCCTGGA ACCCCGCGGA TCACCTGTGC CTTGGCAGGA CGGAAGCCTC 
TCAGACCTGA GTCTGACAGG GGAAGAAATG GCACCTGGAG GCAGCCCTGG GGGCTCAGGC 
TCCGCCCTGA GTGCCCAGTC CACTGAGGCC CTAGAAGGGA TAAGTGGGCG GGGTTCCAAA 
ACTAGTGGAT GTCAGGAGGA GGTGGGCACT CCCAGAAAAG GGCTGGGTGC CCGCCTCCAA 
CAGCTGCTCA CCCCTTCCCG CCGCGCCTCT GCTTCCCGCA TCCCCCCACC TGAGTTGCCC 
TCTGACCTGC CTCCTGCGGC CCGCCGAAGT CCCATGGATA GCCTTCTGTG GCCCCGAGAA 
CGCCCCGGAT CCACTGCTTC TGAGAGCTCT GCTTCTCTGG GCAGTGAGTG GGATATCTCA 
GAGTCTTCCC TCAGCAGCCT GAGTCTCCGC CGTTCCTCAG AGCGTCTCAG TGACACCCCT 
GGGGCTTTCC AGCCACCCTC TCTGGAAATT CTGATGTCTA GCTGTTCCTT GTGTCATGCC 
TGTGATTCCC TGGTGTACGA TGAGGAAATC ATGGCTGGCT GGGCACCGGA TGACTCTAAC 
CTTAATACAA CTTGTCCCTT CTGCGCCTGT CACTTTGTGC CCCTGCTCAG TGTCCAGACT 
CTTGATTCTC GACCCAGTGC ACCTAGTCCA AAGTCTTCCC TTGCTGGCGC CAGTGGCTGC 
AAAGATGCTC CTGCCCCTGG GGGCCCTGGC CCTGTGCTCA GTGACCGGAG GTTCTGCCTT 
GCCCTGGATC AGCCCCAGCT CTGCAATGGG CATATGGGGA GTGCTTCCCG GCGAGTTGAG 
AATGGGGCAT GGGCGTACCT GAGCCCTCTG GTGCTGCGTA AGGAACTGGA GTCCCTGGTA 
GAGAATGAGG GCAGCGAGGT GCTGGCGTTA CCTGAACTGC CTGCTGCCCA CCCTATCATC 
TTCTGGAACC TCCTGTGGTA TTTCCAACGG CTACGCCTGC CTAGTGTTCT ACCAGGCCTG 
GTGTTGGCCT CCTGTAATGG GCCCCCACCC AGCCAGCTCT CCCAGGGACC ATCTCCATGG 
CTAACCCCTG ATCCAGCTTC TGTGCATGTG CATCTGCTGT GGGATGTCCT GACCCCTGAT 
CCCAACAGCT GCCCACCTCT CTACGTGCTC TGGAGGGTCC ACAGCCAGAT CCCACAGCGG 
GTGGTGTGGC CAGGCCCAGT ACCCTCATGT CTTAGCTTGG CATTACTGGA GTCAGTGCTG 
CGCCACGTCG GACTCAACGA GGTTCATAAA GCTGTTGGGC TTCTGCTGGA GACGCTAGGG 
CCCCCTCCCA CCGGCCTGCA CCTACAGAGG GGAATCTACC GTGAGATCTT ATTCCTGACC 
ATGGCTGCTC TGGGCAAGGA CCACGTGGAT ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAAC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CCAAGGCCAT CGACTGCCGA AAGTGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_006501357.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501420.1 Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X5, mRNA.

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CloneID	OMu31831
Clone ID Related Accession (Same CDS sequence)	XM_006501357.1
Accession Version	XM_006501357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCCT ATGACAACCT GCTCCTCTGT CAACCTGTCT CCTCACCCCT GCCCCTCAGT 
GGTGCTAGCT TTTTGCAGCT GCTGCAGAAC CTGGGCCCAG AACTGGCTAT TACATTGCTG 
CTGGCTGTGC TCACAGAGCA CAAACTGCTG GTCCACTCGC TGCGGCCAGA TCTGCTCACC 
AGCGTGTGCG AGGCCCTTGT CTCAATGATC TTCCCTCTGC ATTGGCAGTG TCCCTACATC 
CCATTGTGCC CGCTAGTGCT GGCGGATGTG CTGAGTGCCC CCGTGCCCTT CATTGTGGGT 
ATCCACTCCA GTTATTTCGA TCTGCATGAC CCACCTGCTG ACGTCATCTG TGTTGATCTT 
GATACCAACA CGCTCTTCCA AAAGGAGGAA AAGAAGCCCC TCTCCGCTAG GACCCTGCCC 
CGAAGACCAT ACAAGCTTTT ATTGGCCACC CTGACAAGTC TGTACCAGCA GCTGGACCAG 
ACTTACACTG GACCAGAGGA AGAGGCCTCC CTGGAATTCC TGCTGACAGA CTATGAGGCT 
GTGTGTGGCC GCAGGACTCG CCTGGAACGG GAAGTCCAGG GAGCCTTCCT GCGCTTCATG 
GCCTGTCTGC TCAAAGGTTA CCGGAACTTC CTCCGTCCTC TCACGCAGGC CCCCTCTGAG 
GGGTCTCGAG ATGTTGACAA CCTTTTTTAC CTTCAGGGTT TCCTCAAGTC CCGGGAACGC 
TCAAGCCACA AGCTGTACTC TCAGCTGCTG CACACACAGA TGTTCTCACA GTTCATCGAG 
GAATGCTCTT TTGGCTCCGC CCGGCACGCA GCCCTGGAAT TCTTTGACTC TTGTGTTGAC 
AAGGTTCACC CAGAGCAGGA GAAGCCCGAA CCAACACCCT TAGTGGAGCT GGAGGAACTA 
TCAGGAAGTG AGCTCACTGT CTTTATCACA CCCCCAGAGG AACCACCAGT ACTGGAAGGC 
AGTGAGTCTA CTCCTCAGTA CTGTTATGAT GGGTTTCCAG AGCTAAAGGC TGAGCTATTT 
GAGTCTCCTC AGGAACAACA GGGGGCCCTC CCTGTGCCAG GCCCATCCCG GAGTGCTCCC 
AGCAGTCCTG CACCTCGCCG AACCAAACAG GAGATGAAGG TTGCACAGCG AATGGCACAG 
AAGTCAGCCA CTGTGCCTGA GCTGTGGGCC CGGTGCCTGC TGGGTCACTG CTACGGACTG 
TGGTTCTTAT GCCTGCCCGC CTACGTGCGT TCCGTACCCT CCCGGGTGCG TGCATTACAT 
ACGGCCTACC ACGTGTTACG GGAAATGGAG AACCGCAAGG TGGTGCTCCC TGATGAGGTG 
TGTTACCGCG TGCTGATGCA GCTCTGCTCA CACTATGGGC AGCCCGTTCT GTCTGTGCGA 
GTCATGCTAG AGATGCGACG GGCAGGCATT GTGCCCAACA CCATCACTTA CGGTTACTAC 
AACAAGGCAG TGCTGGAAAG CAAATGGCCA TCTGGTACTC CGGGTGGACG CCTACGCTGG 
GCCAAGCTCC GGAATGTTGT CCTGGGGGCT GCTCAGTTCC GCCAGCCCTT AAAGGACCGA 
AGGCAGCAAC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGA AGCAACAGGT GGCCGAGCAG 
CAAAAGTCGG GCAGCTCTCA GACAGAGCCC TATCTGGAGC GTCCCTCCCC AACGCGCCCT 
CTTCAGCGCC AGACTACTTG GGCTGGGCGA AGTCTACGGG AACCATCTTC ACCCATGGGG 
CGCCTGGTCA AGAGTGGTAG CCTAGGCAGT GCCCGTGGGA CACAGCCCAC GGTGGAGGCT 
GGTGTGGCTC ACATGATAGA GGCCTTGGGG GTCCTGGAAC CCCGCGGATC ACCTGTGCCT 
TGGCAGGACG GAAGCCTCTC AGACCTGAGT CTGACAGGGG AAGAAATGGC ACCTGGAGGC 
AGCCCTGGGG GCTCAGGCTC CGCCCTGAGT GCCCAGTCCA CTGAGGCCCT AGAAGGGATA 
AGTGGGCGGG GTTCCAAAAC TAGTGGATGT CAGGAGGAGG TGGGCACTCC CAGAAAAGGG 
CTGGGTGCCC GCCTCCAACA GCTGCTCACC CCTTCCCGCC GCGCCTCTGC TTCCCGCATC 
CCCCCACCTG AGTTGCCCTC TGACCTGCCT CCTGCGGCCC GCCGAAGTCC CATGGATAGC 
CTTCTGTGGC CCCGAGAACG CCCCGGATCC ACTGCTTCTG AGAGCTCTGC TTCTCTGGGC 
AGTGAGTGGG ATATCTCAGA GTCTTCCCTC AGCAGCCTGA GTCTCCGCCG TTCCTCAGAG 
CGTCTCAGTG ACACCCCTGG GGCTTTCCAG CCACCCTCTC TGGAAATTCT GATGTCTAGC 
TGTTCCTTGT GTCATGCCTG TGATTCCCTG GTGTACGATG AGGAAATCAT GGCTGGCTGG 
GCACCGGATG ACTCTAACCT TAATACAACT TGTCCCTTCT GCGCCTGTCA CTTTGTGCCC 
CTGCTCAGTG TCCAGACTCT TGATTCTCGA CCCAGTGCAC CTAGTCCAAA GTCTTCCCTT 
GCTGGCGCCA GTGGCTGCAA AGATGCTCCT GCCCCTGGGG GCCCTGGCCC TGTGCTCAGT 
GACCGGAGGT TCTGCCTTGC CCTGGATCAG CCCCAGCTCT GCAATGGGCA TATGGGGAGT 
GCTTCCCGGC GAGTTGAGAA TGGGGCATGG GCGTACCTGA GCCCTCTGGT GCTGCGTAAG 
GAACTGGAGT CCCTGGTAGA GAATGAGGGC AGCGAGGTGC TGGCGTTACC TGAACTGCCT 
GCTGCCCACC CTATCATCTT CTGGAACCTC CTGTGGTATT TCCAACGGCT ACGCCTGCCT 
AGTGTTCTAC CAGGCCTGGT GTTGGCCTCC TGTAATGGGC CCCCACCCAG CCAGCTCTCC 
CAGGGACCAT CTCCATGGCT AACCCCTGAT CCAGCTTCTG TGCATGTGCA TCTGCTGTGG 
GATGTCCTGA CCCCTGATCC CAACAGCTGC CCACCTCTCT ACGTGCTCTG GAGGGTCCAC 
AGCCAGATCC CACAGCGGGT GGTGTGGCCA GGCCCAGTAC CCTCATGTCT TAGCTTGGCA 
TTACTGGAGT CAGTGCTGCG CCACGTCGGA CTCAACGAGG TTCATAAAGC TGTTGGGCTT 
CTGCTGGAGA CGCTAGGGCC CCCTCCCACC GGCCTGCACC TACAGAGGGG AATCTACCGT 
GAGATCTTAT TCCTGACCAT GGCTGCTCTG GGCAAGGACC ACGTGGATAT AGTGGCCTTC 
GACAAGAAGT ACAAGTCTGC CTTTAACAAG CTGGCCAGCA GCATGGGCAA GGAGGAACTG 
AGGCAGCGGC GGGCACAGAT GCCCACTCCC AAGGCCATCG ACTGCCGAAA GTGTTTTGGA 
GCACCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501420.1
CDS	462..3839
Translation	MSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQKEEKKPLSARTLPRRPYKLLLATLTSLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSVPSRVRALHTAYHVLREMENRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKDRRQQQQQQQQQQQQKQQVAEQQKSGSSQTEPYLERPSPTRPLQRQTTWAGRSLREPSSPMGRLVKSGSLGSARGTQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEEMAPGGSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEVGTPRKGLGARLQQLLTPSRRASASRIPPPELPSDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACHFVPLLSVQTLDSRPSAPSPKSSLAGASGCKDAPAPGGPGPVLSDRRFCLALDQPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSVLPGLVLASCNGPPPSQLSQGPSPWLTPDPASVHVHLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_006501357.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501357.1

ORF Insert Sequence:

ATGTCTCCCT ATGACAACCT GCTCCTCTGT CAACCTGTCT CCTCACCCCT GCCCCTCAGT 
GGTGCTAGCT TTTTGCAGCT GCTGCAGAAC CTGGGCCCAG AACTGGCTAT TACATTGCTG 
CTGGCTGTGC TCACAGAGCA CAAACTGCTG GTCCACTCGC TGCGGCCAGA TCTGCTCACC 
AGCGTGTGCG AGGCCCTTGT CTCAATGATC TTCCCTCTGC ATTGGCAGTG TCCCTACATC 
CCATTGTGCC CGCTAGTGCT GGCGGATGTG CTGAGTGCCC CCGTGCCCTT CATTGTGGGT 
ATCCACTCCA GTTATTTCGA TCTGCATGAC CCACCTGCTG ACGTCATCTG TGTTGATCTT 
GATACCAACA CGCTCTTCCA AAAGGAGGAA AAGAAGCCCC TCTCCGCTAG GACCCTGCCC 
CGAAGACCAT ACAAGCTTTT ATTGGCCACC CTGACAAGTC TGTACCAGCA GCTGGACCAG 
ACTTACACTG GACCAGAGGA AGAGGCCTCC CTGGAATTCC TGCTGACAGA CTATGAGGCT 
GTGTGTGGCC GCAGGACTCG CCTGGAACGG GAAGTCCAGG GAGCCTTCCT GCGCTTCATG 
GCCTGTCTGC TCAAAGGTTA CCGGAACTTC CTCCGTCCTC TCACGCAGGC CCCCTCTGAG 
GGGTCTCGAG ATGTTGACAA CCTTTTTTAC CTTCAGGGTT TCCTCAAGTC CCGGGAACGC 
TCAAGCCACA AGCTGTACTC TCAGCTGCTG CACACACAGA TGTTCTCACA GTTCATCGAG 
GAATGCTCTT TTGGCTCCGC CCGGCACGCA GCCCTGGAAT TCTTTGACTC TTGTGTTGAC 
AAGGTTCACC CAGAGCAGGA GAAGCCCGAA CCAACACCCT TAGTGGAGCT GGAGGAACTA 
TCAGGAAGTG AGCTCACTGT CTTTATCACA CCCCCAGAGG AACCACCAGT ACTGGAAGGC 
AGTGAGTCTA CTCCTCAGTA CTGTTATGAT GGGTTTCCAG AGCTAAAGGC TGAGCTATTT 
GAGTCTCCTC AGGAACAACA GGGGGCCCTC CCTGTGCCAG GCCCATCCCG GAGTGCTCCC 
AGCAGTCCTG CACCTCGCCG AACCAAACAG GAGATGAAGG TTGCACAGCG AATGGCACAG 
AAGTCAGCCA CTGTGCCTGA GCTGTGGGCC CGGTGCCTGC TGGGTCACTG CTACGGACTG 
TGGTTCTTAT GCCTGCCCGC CTACGTGCGT TCCGTACCCT CCCGGGTGCG TGCATTACAT 
ACGGCCTACC ACGTGTTACG GGAAATGGAG AACCGCAAGG TGGTGCTCCC TGATGAGGTG 
TGTTACCGCG TGCTGATGCA GCTCTGCTCA CACTATGGGC AGCCCGTTCT GTCTGTGCGA 
GTCATGCTAG AGATGCGACG GGCAGGCATT GTGCCCAACA CCATCACTTA CGGTTACTAC 
AACAAGGCAG TGCTGGAAAG CAAATGGCCA TCTGGTACTC CGGGTGGACG CCTACGCTGG 
GCCAAGCTCC GGAATGTTGT CCTGGGGGCT GCTCAGTTCC GCCAGCCCTT AAAGGACCGA 
AGGCAGCAAC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGA AGCAACAGGT GGCCGAGCAG 
CAAAAGTCGG GCAGCTCTCA GACAGAGCCC TATCTGGAGC GTCCCTCCCC AACGCGCCCT 
CTTCAGCGCC AGACTACTTG GGCTGGGCGA AGTCTACGGG AACCATCTTC ACCCATGGGG 
CGCCTGGTCA AGAGTGGTAG CCTAGGCAGT GCCCGTGGGA CACAGCCCAC GGTGGAGGCT 
GGTGTGGCTC ACATGATAGA GGCCTTGGGG GTCCTGGAAC CCCGCGGATC ACCTGTGCCT 
TGGCAGGACG GAAGCCTCTC AGACCTGAGT CTGACAGGGG AAGAAATGGC ACCTGGAGGC 
AGCCCTGGGG GCTCAGGCTC CGCCCTGAGT GCCCAGTCCA CTGAGGCCCT AGAAGGGATA 
AGTGGGCGGG GTTCCAAAAC TAGTGGATGT CAGGAGGAGG TGGGCACTCC CAGAAAAGGG 
CTGGGTGCCC GCCTCCAACA GCTGCTCACC CCTTCCCGCC GCGCCTCTGC TTCCCGCATC 
CCCCCACCTG AGTTGCCCTC TGACCTGCCT CCTGCGGCCC GCCGAAGTCC CATGGATAGC 
CTTCTGTGGC CCCGAGAACG CCCCGGATCC ACTGCTTCTG AGAGCTCTGC TTCTCTGGGC 
AGTGAGTGGG ATATCTCAGA GTCTTCCCTC AGCAGCCTGA GTCTCCGCCG TTCCTCAGAG 
CGTCTCAGTG ACACCCCTGG GGCTTTCCAG CCACCCTCTC TGGAAATTCT GATGTCTAGC 
TGTTCCTTGT GTCATGCCTG TGATTCCCTG GTGTACGATG AGGAAATCAT GGCTGGCTGG 
GCACCGGATG ACTCTAACCT TAATACAACT TGTCCCTTCT GCGCCTGTCA CTTTGTGCCC 
CTGCTCAGTG TCCAGACTCT TGATTCTCGA CCCAGTGCAC CTAGTCCAAA GTCTTCCCTT 
GCTGGCGCCA GTGGCTGCAA AGATGCTCCT GCCCCTGGGG GCCCTGGCCC TGTGCTCAGT 
GACCGGAGGT TCTGCCTTGC CCTGGATCAG CCCCAGCTCT GCAATGGGCA TATGGGGAGT 
GCTTCCCGGC GAGTTGAGAA TGGGGCATGG GCGTACCTGA GCCCTCTGGT GCTGCGTAAG 
GAACTGGAGT CCCTGGTAGA GAATGAGGGC AGCGAGGTGC TGGCGTTACC TGAACTGCCT 
GCTGCCCACC CTATCATCTT CTGGAACCTC CTGTGGTATT TCCAACGGCT ACGCCTGCCT 
AGTGTTCTAC CAGGCCTGGT GTTGGCCTCC TGTAATGGGC CCCCACCCAG CCAGCTCTCC 
CAGGGACCAT CTCCATGGCT AACCCCTGAT CCAGCTTCTG TGCATGTGCA TCTGCTGTGG 
GATGTCCTGA CCCCTGATCC CAACAGCTGC CCACCTCTCT ACGTGCTCTG GAGGGTCCAC 
AGCCAGATCC CACAGCGGGT GGTGTGGCCA GGCCCAGTAC CCTCATGTCT TAGCTTGGCA 
TTACTGGAGT CAGTGCTGCG CCACGTCGGA CTCAACGAGG TTCATAAAGC TGTTGGGCTT 
CTGCTGGAGA CGCTAGGGCC CCCTCCCACC GGCCTGCACC TACAGAGGGG AATCTACCGT 
GAGATCTTAT TCCTGACCAT GGCTGCTCTG GGCAAGGACC ACGTGGATAT AGTGGCCTTC 
GACAAGAAGT ACAAGTCTGC CTTTAACAAG CTGGCCAGCA GCATGGGCAA GGAGGAACTG 
AGGCAGCGGC GGGCACAGAT GCCCACTCCC AAGGCCATCG ACTGCCGAAA GTGTTTTGGA 
GCACCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( NM_201407.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_958809.3 Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23536
Clone ID Related Accession (Same CDS sequence)	NM_201407.4
Accession Version	NM_201407.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Mus musculus(house mouse)
Product	DENN domain-containing protein 4B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119825.11, AK155638.1 and BC066102.1. On Jun 18, 2014 this sequence version replaced NM_201407.3. ##Evidence-Data-START## Transcript exon combination :: AK155638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAG ATGCAGTGAG TGAAGCAGAG GCCATGGCAG AGGAGCGGCC TCCCCGGTTG 
GTGGATTACT TTGTGGTGGC TGGGCTTGCA GGAAATGGAG CGCCTATACC TGAGGAGAAG 
TGGGTTCCCG AGCCTACCGG GCCCCTGCGC CCTCCCCGGC CAGCTGAACC TATTACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTATAC ATGCATTCAG 
ACTTCTGCGG GTGGCCACCC CTTGGAGCTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGCAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTATAT 
GAAGGGAAGG AACGACCCAA GCTTGGGTTC CAAGTGCTTG ACACGACACC GTATAGCCAC 
TCAGCCAATC TGGCCCCTCC AGGCCCCGGG CACCCCCGCA CCTACCTCAT GTATCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTTCTGCCC 
AGCAAGGGTG AGGGTACTCC TCATACTTAC TGCCGGCTAC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTAGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAAG ACAATGAGGC GTTTCCGCTG 
CCCGAGTCAG TCCCTGTCTT CTGTCTGCCC ATGGGGGCCA CTATTGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCAGTACC TGTCTTCTCC ACCTTTGTGC TCACAGGCGC TGCTGGTGAT 
AAGGTGTATG GTGCTGCTTT GCAGTTCTAT GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTGATGAGT GCCGTGGAAC GGGGCCGGGC GCTGGGAGGC 
CGAGCTGTGC GCAGCCGACG AGCCATAGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGAGCCT TCCTCACCTT CCTTTACCGA TACTCCGTCT CAGGCCCCCA TCGTCTGCCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCAT TCCCATCCCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGTCAACC TGTCTCCTCA 
CCCCTGCCCC TCAGTGGTGC TAGCTTTTTG CAGCTGCTGC AGAACCTGGG CCCAGAACTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT GTGCGAGGCC CTTGTCTCAA TGATCTTCCC TCTGCATTGG 
CAGTGTCCCT ACATCCCATT GTGCCCGCTA GTGCTGGCGG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGTTAT TTCGATCTGC ATGACCCACC TGCTGACGTC 
ATCTGTGTTG ATCTTGATAC CAACACGCTC TTCCAAAAGG AGGAAAAGAA GCCCCTCTCC 
GCTAGGACCC TGCCCCGAAG ACCATACAAG CTTTTATTGG CCACCCTGAC AAGTCTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTATG AGGCTGTGTG TGGCCGCAGG ACTCGCCTGG AACGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGTTACCGGA ACTTCCTCCG TCCTCTCACG 
CAGGCCCCCT CTGAGGGGTC TCGAGATGTT GACAACCTTT TTTACCTTCA GGGTTTCCTC 
AAGTCCCGGG AACGCTCAAG CCACAAGCTG TACTCTCAGC TGCTGCACAC ACAGATGTTC 
TCACAGTTCA TCGAGGAATG CTCTTTTGGC TCCGCCCGGC ACGCAGCCCT GGAATTCTTT 
GACTCTTGTG TTGACAAGGT TCACCCAGAG CAGGAGAAGC CCGAACCAAC ACCCTTAGTG 
GAGCTGGAGG AACTATCAGG AAGTGAGCTC ACTGTCTTTA TCACACCCCC AGAGGAACCA 
CCAGTACTGG AAGGCAGTGA GTCTACTCCT CAGTACTGTT ATGATGGGTT TCCAGAGCTA 
AAGGCTGAGC TATTTGAGTC TCCTCAGGAA CAACAGGGGG CCCTCCCTGT GCCAGGCCCA 
TCCCGGAGTG CTCCCAGCAG TCCTGCACCT CGCCGAACCA AACAGGAGAT GAAGGTTGCA 
CAGCGAATGG CACAGAAGTC AGCCACTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT 
CACTGCTACG GACTGTGGTT CTTATGCCTG CCCGCCTACG TGCGTTCCGT ACCCTCCCGG 
GTGCGTGCAT TACATACGGC CTACCACGTG TTACGGGAAA TGGAGAACCG CAAGGTGGTG 
CTCCCTGATG AGGTGTGTTA CCGCGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCC 
GTTCTGTCTG TGCGAGTCAT GCTAGAGATG CGACGGGCAG GCATTGTGCC CAACACCATC 
ACTTACGGTT ACTACAACAA GGCAGTGCTG GAAAGCAAAT GGCCATCTGG TACTCCGGGT 
GGACGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTAAAGG ACCGAAGGCA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGAAGCAA 
CAGGTGGCCG AGCAGCAAAA GTCGGGCAGC TCTCAGACAG AGCCCTATCT GGAGCGTCCC 
TCCCCAACGC GCCCTCTTCA GCGCCAGACT ACTTGGGCTG GGCGAAGTCT ACGGGAACCA 
TCTTCACCCA TGGGGCGCCT GGTCAAGAGT GGTAGCCTAG GCAGTGCCCG TGGGACACAG 
CCCACGGTGG AGGCTGGTGT GGCTCACATG ATAGAGGCCT TGGGGGTCCT GGAACCCCGC 
GGATCACCTG TGCCTTGGCA GGACGGAAGC CTCTCAGACC TGAGTCTGAC AGGGGAAGAA 
ATGGCACCTG GAGGCAGCCC TGGGGGCTCA GGCTCCGCCC TGAGTGCCCA GTCCACTGAG 
GCCCTAGAAG GGATAAGTGG GCGGGGTTCC AAAACTAGTG GATGTCAGGA GGAGGTGGGC 
ACTCCCAGAA AAGGGCTGGG TGCCCGCCTC CAACAGCTGC TCACCCCTTC CCGCCGCGCC 
TCTGCTTCCC GCATCCCCCC ACCTGAGTTG CCCTCTGACC TGCCTCCTGC GGCCCGCCGA 
AGTCCCATGG ATAGCCTTCT GTGGCCCCGA GAACGCCCCG GATCCACTGC TTCTGAGAGC 
TCTGCTTCTC TGGGCAGTGA GTGGGATATC TCAGAGTCTT CCCTCAGCAG CCTGAGTCTC 
CGCCGTTCCT CAGAGCGTCT CAGTGACACC CCTGGGGCTT TCCAGCCACC CTCTCTGGAA 
ATTCTGATGT CTAGCTGTTC CTTGTGTCAT GCCTGTGATT CCCTGGTGTA CGATGAGGAA 
ATCATGGCTG GCTGGGCACC GGATGACTCT AACCTTAATA CAACTTGTCC CTTCTGCGCC 
TGTCACTTTG TGCCCCTGCT CAGTGTCCAG ACTCTTGATT CTCGACCCAG TGCACCTAGT 
CCAAAGTCTT CCCTTGCTGG CGCCAGTGGC TGCAAAGATG CTCCTGCCCC TGGGGGCCCT 
GGCCCTGTGC TCAGTGACCG GAGGTTCTGC CTTGCCCTGG ATCAGCCCCA GCTCTGCAAT 
GGGCATATGG GGAGTGCTTC CCGGCGAGTT GAGAATGGGG CATGGGCGTA CCTGAGCCCT 
CTGGTGCTGC GTAAGGAACT GGAGTCCCTG GTAGAGAATG AGGGCAGCGA GGTGCTGGCG 
TTACCTGAAC TGCCTGCTGC CCACCCTATC ATCTTCTGGA ACCTCCTGTG GTATTTCCAA 
CGGCTACGCC TGCCTAGTGT TCTACCAGGC CTGGTGTTGG CCTCCTGTAA TGGGCCCCCA 
CCCAGCCAGC TCTCCCAGGG ACCATCTCCA TGGCTAACCC CTGATCCAGC TTCTGTGCAT 
GTGCATCTGC TGTGGGATGT CCTGACCCCT GATCCCAACA GCTGCCCACC TCTCTACGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTGT GGCCAGGCCC AGTACCCTCA 
TGTCTTAGCT TGGCATTACT GGAGTCAGTG CTGCGCCACG TCGGACTCAA CGAGGTTCAT 
AAAGCTGTTG GGCTTCTGCT GGAGACGCTA GGGCCCCCTC CCACCGGCCT GCACCTACAG 
AGGGGAATCT ACCGTGAGAT CTTATTCCTG ACCATGGCTG CTCTGGGCAA GGACCACGTG 
GATATAGTGG CCTTCGACAA GAAGTACAAG TCTGCCTTTA ACAAGCTGGC CAGCAGCATG 
GGCAAGGAGG AACTGAGGCA GCGGCGGGCA CAGATGCCCA CTCCCAAGGC CATCGACTGC 
CGAAAGTGTT TTGGAGCACC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958809.3
CDS	144..4676
Translation	MEADAVSEAEAMAEERPPRLVDYFVVAGLAGNGAPIPEEKWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLMSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQKEEKKPLSARTLPRRPYKLLLATLTSLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSVPSRVRALHTAYHVLREMENRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKDRRQQQQQQQQQQQQKQQVAEQQKSGSSQTEPYLERPSPTRPLQRQTTWAGRSLREPSSPMGRLVKSGSLGSARGTQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEEMAPGGSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEVGTPRKGLGARLQQLLTPSRRASASRIPPPELPSDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACHFVPLLSVQTLDSRPSAPSPKSSLAGASGCKDAPAPGGPGPVLSDRRFCLALDQPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSVLPGLVLASCNGPPPSQLSQGPSPWLTPDPASVHVHLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	NM_201407.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201407.4

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAAGCAGAG GCCATGGCAG AGGAGCGGCC TCCCCGGTTG 
GTGGATTACT TTGTGGTGGC TGGGCTTGCA GGAAATGGAG CGCCTATACC TGAGGAGAAG 
TGGGTTCCCG AGCCTACCGG GCCCCTGCGC CCTCCCCGGC CAGCTGAACC TATTACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTATAC ATGCATTCAG 
ACTTCTGCGG GTGGCCACCC CTTGGAGCTC AGTGCTGGGC TCCTGGGTGG AACTCAACCA 
GTCATCTGCT ACCGCAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTATAT 
GAAGGGAAGG AACGACCCAA GCTTGGGTTC CAAGTGCTTG ACACGACACC GTATAGCCAC 
TCAGCCAATC TGGCCCCTCC AGGCCCCGGG CACCCCCGCA CCTACCTCAT GTATCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTTCTGCCC 
AGCAAGGGTG AGGGTACTCC TCATACTTAC TGCCGGCTAC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTAGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAAG ACAATGAGGC GTTTCCGCTG 
CCCGAGTCAG TCCCTGTCTT CTGTCTGCCC ATGGGGGCCA CTATTGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCAGTACC TGTCTTCTCC ACCTTTGTGC TCACAGGCGC TGCTGGTGAT 
AAGGTGTATG GTGCTGCTTT GCAGTTCTAT GAAGCGTTCC CGAGGGCCAG GCTGTCAGAG 
AGGCAGGCAC GGGCACTGGG CCTGATGAGT GCCGTGGAAC GGGGCCGGGC GCTGGGAGGC 
CGAGCTGTGC GCAGCCGACG AGCCATAGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGAGCCT TCCTCACCTT CCTTTACCGA TACTCCGTCT CAGGCCCCCA TCGTCTGCCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCAT TCCCATCCCC ACAGAGACCA 
CGCATCCTAG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGTCAACC TGTCTCCTCA 
CCCCTGCCCC TCAGTGGTGC TAGCTTTTTG CAGCTGCTGC AGAACCTGGG CCCAGAACTG 
GCTATTACAT TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTGGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT GTGCGAGGCC CTTGTCTCAA TGATCTTCCC TCTGCATTGG 
CAGTGTCCCT ACATCCCATT GTGCCCGCTA GTGCTGGCGG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGTTAT TTCGATCTGC ATGACCCACC TGCTGACGTC 
ATCTGTGTTG ATCTTGATAC CAACACGCTC TTCCAAAAGG AGGAAAAGAA GCCCCTCTCC 
GCTAGGACCC TGCCCCGAAG ACCATACAAG CTTTTATTGG CCACCCTGAC AAGTCTGTAC 
CAGCAGCTGG ACCAGACTTA CACTGGACCA GAGGAAGAGG CCTCCCTGGA ATTCCTGCTG 
ACAGACTATG AGGCTGTGTG TGGCCGCAGG ACTCGCCTGG AACGGGAAGT CCAGGGAGCC 
TTCCTGCGCT TCATGGCCTG TCTGCTCAAA GGTTACCGGA ACTTCCTCCG TCCTCTCACG 
CAGGCCCCCT CTGAGGGGTC TCGAGATGTT GACAACCTTT TTTACCTTCA GGGTTTCCTC 
AAGTCCCGGG AACGCTCAAG CCACAAGCTG TACTCTCAGC TGCTGCACAC ACAGATGTTC 
TCACAGTTCA TCGAGGAATG CTCTTTTGGC TCCGCCCGGC ACGCAGCCCT GGAATTCTTT 
GACTCTTGTG TTGACAAGGT TCACCCAGAG CAGGAGAAGC CCGAACCAAC ACCCTTAGTG 
GAGCTGGAGG AACTATCAGG AAGTGAGCTC ACTGTCTTTA TCACACCCCC AGAGGAACCA 
CCAGTACTGG AAGGCAGTGA GTCTACTCCT CAGTACTGTT ATGATGGGTT TCCAGAGCTA 
AAGGCTGAGC TATTTGAGTC TCCTCAGGAA CAACAGGGGG CCCTCCCTGT GCCAGGCCCA 
TCCCGGAGTG CTCCCAGCAG TCCTGCACCT CGCCGAACCA AACAGGAGAT GAAGGTTGCA 
CAGCGAATGG CACAGAAGTC AGCCACTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGT 
CACTGCTACG GACTGTGGTT CTTATGCCTG CCCGCCTACG TGCGTTCCGT ACCCTCCCGG 
GTGCGTGCAT TACATACGGC CTACCACGTG TTACGGGAAA TGGAGAACCG CAAGGTGGTG 
CTCCCTGATG AGGTGTGTTA CCGCGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCC 
GTTCTGTCTG TGCGAGTCAT GCTAGAGATG CGACGGGCAG GCATTGTGCC CAACACCATC 
ACTTACGGTT ACTACAACAA GGCAGTGCTG GAAAGCAAAT GGCCATCTGG TACTCCGGGT 
GGACGCCTAC GCTGGGCCAA GCTCCGGAAT GTTGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTAAAGG ACCGAAGGCA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGAAGCAA 
CAGGTGGCCG AGCAGCAAAA GTCGGGCAGC TCTCAGACAG AGCCCTATCT GGAGCGTCCC 
TCCCCAACGC GCCCTCTTCA GCGCCAGACT ACTTGGGCTG GGCGAAGTCT ACGGGAACCA 
TCTTCACCCA TGGGGCGCCT GGTCAAGAGT GGTAGCCTAG GCAGTGCCCG TGGGACACAG 
CCCACGGTGG AGGCTGGTGT GGCTCACATG ATAGAGGCCT TGGGGGTCCT GGAACCCCGC 
GGATCACCTG TGCCTTGGCA GGACGGAAGC CTCTCAGACC TGAGTCTGAC AGGGGAAGAA 
ATGGCACCTG GAGGCAGCCC TGGGGGCTCA GGCTCCGCCC TGAGTGCCCA GTCCACTGAG 
GCCCTAGAAG GGATAAGTGG GCGGGGTTCC AAAACTAGTG GATGTCAGGA GGAGGTGGGC 
ACTCCCAGAA AAGGGCTGGG TGCCCGCCTC CAACAGCTGC TCACCCCTTC CCGCCGCGCC 
TCTGCTTCCC GCATCCCCCC ACCTGAGTTG CCCTCTGACC TGCCTCCTGC GGCCCGCCGA 
AGTCCCATGG ATAGCCTTCT GTGGCCCCGA GAACGCCCCG GATCCACTGC TTCTGAGAGC 
TCTGCTTCTC TGGGCAGTGA GTGGGATATC TCAGAGTCTT CCCTCAGCAG CCTGAGTCTC 
CGCCGTTCCT CAGAGCGTCT CAGTGACACC CCTGGGGCTT TCCAGCCACC CTCTCTGGAA 
ATTCTGATGT CTAGCTGTTC CTTGTGTCAT GCCTGTGATT CCCTGGTGTA CGATGAGGAA 
ATCATGGCTG GCTGGGCACC GGATGACTCT AACCTTAATA CAACTTGTCC CTTCTGCGCC 
TGTCACTTTG TGCCCCTGCT CAGTGTCCAG ACTCTTGATT CTCGACCCAG TGCACCTAGT 
CCAAAGTCTT CCCTTGCTGG CGCCAGTGGC TGCAAAGATG CTCCTGCCCC TGGGGGCCCT 
GGCCCTGTGC TCAGTGACCG GAGGTTCTGC CTTGCCCTGG ATCAGCCCCA GCTCTGCAAT 
GGGCATATGG GGAGTGCTTC CCGGCGAGTT GAGAATGGGG CATGGGCGTA CCTGAGCCCT 
CTGGTGCTGC GTAAGGAACT GGAGTCCCTG GTAGAGAATG AGGGCAGCGA GGTGCTGGCG 
TTACCTGAAC TGCCTGCTGC CCACCCTATC ATCTTCTGGA ACCTCCTGTG GTATTTCCAA 
CGGCTACGCC TGCCTAGTGT TCTACCAGGC CTGGTGTTGG CCTCCTGTAA TGGGCCCCCA 
CCCAGCCAGC TCTCCCAGGG ACCATCTCCA TGGCTAACCC CTGATCCAGC TTCTGTGCAT 
GTGCATCTGC TGTGGGATGT CCTGACCCCT GATCCCAACA GCTGCCCACC TCTCTACGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTGT GGCCAGGCCC AGTACCCTCA 
TGTCTTAGCT TGGCATTACT GGAGTCAGTG CTGCGCCACG TCGGACTCAA CGAGGTTCAT 
AAAGCTGTTG GGCTTCTGCT GGAGACGCTA GGGCCCCCTC CCACCGGCCT GCACCTACAG 
AGGGGAATCT ACCGTGAGAT CTTATTCCTG ACCATGGCTG CTCTGGGCAA GGACCACGTG 
GATATAGTGG CCTTCGACAA GAAGTACAAG TCTGCCTTTA ACAAGCTGGC CAGCAGCATG 
GGCAAGGAGG AACTGAGGCA GCGGCGGGCA CAGATGCCCA CTCCCAAGGC CATCGACTGC 
CGAAAGTGTT TTGGAGCACC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_017319543.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175032.1 Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X1, mRNA.

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CloneID	OMu80598
Clone ID Related Accession (Same CDS sequence)	XM_017319543.1 , XM_017319543.2
Accession Version	XM_017319543.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACAT TATCCTCTCT TGCTCCATCT CTTCCTCGAT TGACCATTCC AGATGCAGTG 
AGTGAAGCAG AGGCCATGGC AGAGGAGCGG CCTCCCCGGT TGGTGGATTA CTTTGTGGTG 
GCTGGGCTTG CAGGAAATGG AGCGCCTATA CCTGAGGAGA AGTGGGTTCC CGAGCCTACC 
GGGCCCCTGC GCCCTCCCCG GCCAGCTGAA CCTATTACAG ATGTGGCAGT CATCGCTAGG 
GCACTGGGCG AGGAGGTGCC CCAGGGCTAT ACATGCATTC AGACTTCTGC GGGTGGCCAC 
CCCTTGGAGC TCAGTGCTGG GCTCCTGGGT GGAACTCAAC CAGTCATCTG CTACCGCAGG 
GGGCGTGACA AGCCCCCCCT CGTTGAGCTG GGGGTGTTAT ATGAAGGGAA GGAACGACCC 
AAGCTTGGGT TCCAAGTGCT TGACACGACA CCGTATAGCC ACTCAGCCAA TCTGGCCCCT 
CCAGGCCCCG GGCACCCCCG CACCTACCTC ATGTATCGGC GGGCAGCAGA AGGGGCAGGG 
CTGCATGCCC TGGGCATCAC TGACCTCTGC CTGGTTCTGC CCAGCAAGGG TGAGGGTACT 
CCTCATACTT ACTGCCGGCT ACCCCGCAAC CTCAACCCTG GCATGTGGGG CCCAGCAGTG 
TACCTGTGCT ACAAGGTAGG CCTGGCCAAG GCCAACACAC TGGTGTATGA GGCAGAGCTG 
CTGGGCCGCT ACCCAGAGGA AGACAATGAG GCGTTTCCGC TGCCCGAGTC AGTCCCTGTC 
TTCTGTCTGC CCATGGGGGC CACTATTGAG TGCTGGCCTG CCCAGACCAA GTACCCAGTA 
CCTGTCTTCT CCACCTTTGT GCTCACAGGC GCTGCTGGTG ATAAGGTGTA TGGTGCTGCT 
TTGCAGTTCT ATGAAGCGTT CCCGAGGGCC AGGCTGTCAG AGAGGCAGGC ACGGGCACTG 
GGCCTGATGA GTGCCGTGGA ACGGGGCCGG GCGCTGGGAG GCCGAGCTGT GCGCAGCCGA 
CGAGCCATAG CTGTGCTGTC CCGCTGGCCT GCCTTCCCAG CCTTCCGAGC CTTCCTCACC 
TTCCTTTACC GATACTCCGT CTCAGGCCCC CATCGTCTGC CCTTGGAAGC GCACATCTCC 
CACTTCATTC ACAACGTCCC ATTCCCATCC CCACAGAGAC CACGCATCCT AGTGCAGATG 
TCTCCCTATG ACAACCTGCT CCTCTGTCAA CCTGTCTCCT CACCCCTGCC CCTCAGTGGT 
GCTAGCTTTT TGCAGCTGCT GCAGAACCTG GGCCCAGAAC TGGCTATTAC ATTGCTGCTG 
GCTGTGCTCA CAGAGCACAA ACTGCTGGTC CACTCGCTGC GGCCAGATCT GCTCACCAGC 
GTGTGCGAGG CCCTTGTCTC AATGATCTTC CCTCTGCATT GGCAGTGTCC CTACATCCCA 
TTGTGCCCGC TAGTGCTGGC GGATGTGCTG AGTGCCCCCG TGCCCTTCAT TGTGGGTATC 
CACTCCAGTT ATTTCGATCT GCATGACCCA CCTGCTGACG TCATCTGTGT TGATCTTGAT 
ACCAACACGC TCTTCCAAAA GGAGGAAAAG AAGCCCCTCT CCGCTAGGAC CCTGCCCCGA 
AGACCATACA AGCTTTTATT GGCCACCCTG ACAAGTCTGT ACCAGCAGCT GGACCAGACT 
TACACTGGAC CAGAGGAAGA GGCCTCCCTG GAATTCCTGC TGACAGACTA TGAGGCTGTG 
TGTGGCCGCA GGACTCGCCT GGAACGGGAA GTCCAGGGAG CCTTCCTGCG CTTCATGGCC 
TGTCTGCTCA AAGGTTACCG GAACTTCCTC CGTCCTCTCA CGCAGGCCCC CTCTGAGGGG 
TCTCGAGATG TTGACAACCT TTTTTACCTT CAGGGTTTCC TCAAGTCCCG GGAACGCTCA 
AGCCACAAGC TGTACTCTCA GCTGCTGCAC ACACAGATGT TCTCACAGTT CATCGAGGAA 
TGCTCTTTTG GCTCCGCCCG GCACGCAGCC CTGGAATTCT TTGACTCTTG TGTTGACAAG 
GTTCACCCAG AGCAGGAGAA GCCCGAACCA ACACCCTTAG TGGAGCTGGA GGAACTATCA 
GGAAGTGAGC TCACTGTCTT TATCACACCC CCAGAGGAAC CACCAGTACT GGAAGGCAGT 
GAGTCTACTC CTCAGTACTG TTATGATGGG TTTCCAGAGC TAAAGGCTGA GCTATTTGAG 
TCTCCTCAGG AACAACAGGG GGCCCTCCCT GTGCCAGGCC CATCCCGGAG TGCTCCCAGC 
AGTCCTGCAC CTCGCCGAAC CAAACAGGAG ATGAAGGTTG CACAGCGAAT GGCACAGAAG 
TCAGCCACTG TGCCTGAGCT GTGGGCCCGG TGCCTGCTGG GTCACTGCTA CGGACTGTGG 
TTCTTATGCC TGCCCGCCTA CGTGCGTTCC GTACCCTCCC GGGTGCGTGC ATTACATACG 
GCCTACCACG TGTTACGGGA AATGGAGAAC CGCAAGGTGG TGCTCCCTGA TGAGGTGTGT 
TACCGCGTGC TGATGCAGCT CTGCTCACAC TATGGGCAGC CCGTTCTGTC TGTGCGAGTC 
ATGCTAGAGA TGCGACGGGC AGGCATTGTG CCCAACACCA TCACTTACGG TTACTACAAC 
AAGGCAGTGC TGGAAAGCAA ATGGCCATCT GGTACTCCGG GTGGACGCCT ACGCTGGGCC 
AAGCTCCGGA ATGTTGTCCT GGGGGCTGCT CAGTTCCGCC AGCCCTTAAA GGACCGAAGG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAG CAGCAGAAGC AACAGGTGGC CGAGCAGCAA 
AAGTCGGGCA GCTCTCAGAC AGAGCCCTAT CTGGAGCGTC CCTCCCCAAC GCGCCCTCTT 
CAGCGCCAGA CTACTTGGGC TGGGCGAAGT CTACGGGAAC CATCTTCACC CATGGGGCGC 
CTGGTCAAGA GTGGTAGCCT AGGCAGTGCC CGTGGGACAC AGCCCACGGT GGAGGCTGGT 
GTGGCTCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GCGGATCACC TGTGCCTTGG 
CAGGACGGAA GCCTCTCAGA CCTGAGTCTG ACAGGGGAAG AAATGGCACC TGGAGGCAGC 
CCTGGGGGCT CAGGCTCCGC CCTGAGTGCC CAGTCCACTG AGGCCCTAGA AGGGATAAGT 
GGGCGGGGTT CCAAAACTAG TGGATGTCAG GAGGAGGTGG GCACTCCCAG AAAAGGGCTG 
GGTGCCCGCC TCCAACAGCT GCTCACCCCT TCCCGCCGCG CCTCTGCTTC CCGCATCCCC 
CCACCTGAGT TGCCCTCTGA CCTGCCTCCT GCGGCCCGCC GAAGTCCCAT GGATAGCCTT 
CTGTGGCCCC GAGAACGCCC CGGATCCACT GCTTCTGAGA GCTCTGCTTC TCTGGGCAGT 
GAGTGGGATA TCTCAGAGTC TTCCCTCAGC AGCCTGAGTC TCCGCCGTTC CTCAGAGCGT 
CTCAGTGACA CCCCTGGGGC TTTCCAGCCA CCCTCTCTGG AAATTCTGAT GTCTAGCTGT 
TCCTTGTGTC ATGCCTGTGA TTCCCTGGTG TACGATGAGG AAATCATGGC TGGCTGGGCA 
CCGGATGACT CTAACCTTAA TACAACTTGT CCCTTCTGCG CCTGTCACTT TGTGCCCCTG 
CTCAGTGTCC AGACTCTTGA TTCTCGACCC AGTGCACCTA GTCCAAAGTC TTCCCTTGCT 
GGCGCCAGTG GCTGCAAAGA TGCTCCTGCC CCTGGGGGCC CTGGCCCTGT GCTCAGTGAC 
CGGAGGTTCT GCCTTGCCCT GGATCAGCCC CAGCTCTGCA ATGGGCATAT GGGGAGTGCT 
TCCCGGCGAG TTGAGAATGG GGCATGGGCG TACCTGAGCC CTCTGGTGCT GCGTAAGGAA 
CTGGAGTCCC TGGTAGAGAA TGAGGGCAGC GAGGTGCTGG CGTTACCTGA ACTGCCTGCT 
GCCCACCCTA TCATCTTCTG GAACCTCCTG TGGTATTTCC AACGGCTACG CCTGCCTAGT 
GTTCTACCAG GCCTGGTGTT GGCCTCCTGT AATGGGCCCC CACCCAGCCA GCTCTCCCAG 
GGACCATCTC CATGGCTAAC CCCTGATCCA GCTTCTGTGC ATGTGCATCT GCTGTGGGAT 
GTCCTGACCC CTGATCCCAA CAGCTGCCCA CCTCTCTACG TGCTCTGGAG GGTCCACAGC 
CAGATCCCAC AGCGGGTGGT GTGGCCAGGC CCAGTACCCT CATGTCTTAG CTTGGCATTA 
CTGGAGTCAG TGCTGCGCCA CGTCGGACTC AACGAGGTTC ATAAAGCTGT TGGGCTTCTG 
CTGGAGACGC TAGGGCCCCC TCCCACCGGC CTGCACCTAC AGAGGGGAAT CTACCGTGAG 
ATCTTATTCC TGACCATGGC TGCTCTGGGC AAGGACCACG TGGATATAGT GGCCTTCGAC 
AAGAAGTACA AGTCTGCCTT TAACAAGCTG GCCAGCAGCA TGGGCAAGGA GGAACTGAGG 
CAGCGGCGGG CACAGATGCC CACTCCCAAG GCCATCGACT GCCGAAAGTG TTTTGGAGCA 
CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175032.1
CDS	669..5243
Translation	MGTLSSLAPSLPRLTIPDAVSEAEAMAEERPPRLVDYFVVAGLAGNGAPIPEEKWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLMSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQKEEKKPLSARTLPRRPYKLLLATLTSLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSVPSRVRALHTAYHVLREMENRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKDRRQQQQQQQQQQQQKQQVAEQQKSGSSQTEPYLERPSPTRPLQRQTTWAGRSLREPSSPMGRLVKSGSLGSARGTQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEEMAPGGSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEVGTPRKGLGARLQQLLTPSRRASASRIPPPELPSDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACHFVPLLSVQTLDSRPSAPSPKSSLAGASGCKDAPAPGGPGPVLSDRRFCLALDQPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSVLPGLVLASCNGPPPSQLSQGPSPWLTPDPASVHVHLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_017319543.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319543.1

ORF Insert Sequence:

ATGGGAACAT TATCCTCTCT TGCTCCATCT CTTCCTCGAT TGACCATTCC AGATGCAGTG 
AGTGAAGCAG AGGCCATGGC AGAGGAGCGG CCTCCCCGGT TGGTGGATTA CTTTGTGGTG 
GCTGGGCTTG CAGGAAATGG AGCGCCTATA CCTGAGGAGA AGTGGGTTCC CGAGCCTACC 
GGGCCCCTGC GCCCTCCCCG GCCAGCTGAA CCTATTACAG ATGTGGCAGT CATCGCTAGG 
GCACTGGGCG AGGAGGTGCC CCAGGGCTAT ACATGCATTC AGACTTCTGC GGGTGGCCAC 
CCCTTGGAGC TCAGTGCTGG GCTCCTGGGT GGAACTCAAC CAGTCATCTG CTACCGCAGG 
GGGCGTGACA AGCCCCCCCT CGTTGAGCTG GGGGTGTTAT ATGAAGGGAA GGAACGACCC 
AAGCTTGGGT TCCAAGTGCT TGACACGACA CCGTATAGCC ACTCAGCCAA TCTGGCCCCT 
CCAGGCCCCG GGCACCCCCG CACCTACCTC ATGTATCGGC GGGCAGCAGA AGGGGCAGGG 
CTGCATGCCC TGGGCATCAC TGACCTCTGC CTGGTTCTGC CCAGCAAGGG TGAGGGTACT 
CCTCATACTT ACTGCCGGCT ACCCCGCAAC CTCAACCCTG GCATGTGGGG CCCAGCAGTG 
TACCTGTGCT ACAAGGTAGG CCTGGCCAAG GCCAACACAC TGGTGTATGA GGCAGAGCTG 
CTGGGCCGCT ACCCAGAGGA AGACAATGAG GCGTTTCCGC TGCCCGAGTC AGTCCCTGTC 
TTCTGTCTGC CCATGGGGGC CACTATTGAG TGCTGGCCTG CCCAGACCAA GTACCCAGTA 
CCTGTCTTCT CCACCTTTGT GCTCACAGGC GCTGCTGGTG ATAAGGTGTA TGGTGCTGCT 
TTGCAGTTCT ATGAAGCGTT CCCGAGGGCC AGGCTGTCAG AGAGGCAGGC ACGGGCACTG 
GGCCTGATGA GTGCCGTGGA ACGGGGCCGG GCGCTGGGAG GCCGAGCTGT GCGCAGCCGA 
CGAGCCATAG CTGTGCTGTC CCGCTGGCCT GCCTTCCCAG CCTTCCGAGC CTTCCTCACC 
TTCCTTTACC GATACTCCGT CTCAGGCCCC CATCGTCTGC CCTTGGAAGC GCACATCTCC 
CACTTCATTC ACAACGTCCC ATTCCCATCC CCACAGAGAC CACGCATCCT AGTGCAGATG 
TCTCCCTATG ACAACCTGCT CCTCTGTCAA CCTGTCTCCT CACCCCTGCC CCTCAGTGGT 
GCTAGCTTTT TGCAGCTGCT GCAGAACCTG GGCCCAGAAC TGGCTATTAC ATTGCTGCTG 
GCTGTGCTCA CAGAGCACAA ACTGCTGGTC CACTCGCTGC GGCCAGATCT GCTCACCAGC 
GTGTGCGAGG CCCTTGTCTC AATGATCTTC CCTCTGCATT GGCAGTGTCC CTACATCCCA 
TTGTGCCCGC TAGTGCTGGC GGATGTGCTG AGTGCCCCCG TGCCCTTCAT TGTGGGTATC 
CACTCCAGTT ATTTCGATCT GCATGACCCA CCTGCTGACG TCATCTGTGT TGATCTTGAT 
ACCAACACGC TCTTCCAAAA GGAGGAAAAG AAGCCCCTCT CCGCTAGGAC CCTGCCCCGA 
AGACCATACA AGCTTTTATT GGCCACCCTG ACAAGTCTGT ACCAGCAGCT GGACCAGACT 
TACACTGGAC CAGAGGAAGA GGCCTCCCTG GAATTCCTGC TGACAGACTA TGAGGCTGTG 
TGTGGCCGCA GGACTCGCCT GGAACGGGAA GTCCAGGGAG CCTTCCTGCG CTTCATGGCC 
TGTCTGCTCA AAGGTTACCG GAACTTCCTC CGTCCTCTCA CGCAGGCCCC CTCTGAGGGG 
TCTCGAGATG TTGACAACCT TTTTTACCTT CAGGGTTTCC TCAAGTCCCG GGAACGCTCA 
AGCCACAAGC TGTACTCTCA GCTGCTGCAC ACACAGATGT TCTCACAGTT CATCGAGGAA 
TGCTCTTTTG GCTCCGCCCG GCACGCAGCC CTGGAATTCT TTGACTCTTG TGTTGACAAG 
GTTCACCCAG AGCAGGAGAA GCCCGAACCA ACACCCTTAG TGGAGCTGGA GGAACTATCA 
GGAAGTGAGC TCACTGTCTT TATCACACCC CCAGAGGAAC CACCAGTACT GGAAGGCAGT 
GAGTCTACTC CTCAGTACTG TTATGATGGG TTTCCAGAGC TAAAGGCTGA GCTATTTGAG 
TCTCCTCAGG AACAACAGGG GGCCCTCCCT GTGCCAGGCC CATCCCGGAG TGCTCCCAGC 
AGTCCTGCAC CTCGCCGAAC CAAACAGGAG ATGAAGGTTG CACAGCGAAT GGCACAGAAG 
TCAGCCACTG TGCCTGAGCT GTGGGCCCGG TGCCTGCTGG GTCACTGCTA CGGACTGTGG 
TTCTTATGCC TGCCCGCCTA CGTGCGTTCC GTACCCTCCC GGGTGCGTGC ATTACATACG 
GCCTACCACG TGTTACGGGA AATGGAGAAC CGCAAGGTGG TGCTCCCTGA TGAGGTGTGT 
TACCGCGTGC TGATGCAGCT CTGCTCACAC TATGGGCAGC CCGTTCTGTC TGTGCGAGTC 
ATGCTAGAGA TGCGACGGGC AGGCATTGTG CCCAACACCA TCACTTACGG TTACTACAAC 
AAGGCAGTGC TGGAAAGCAA ATGGCCATCT GGTACTCCGG GTGGACGCCT ACGCTGGGCC 
AAGCTCCGGA ATGTTGTCCT GGGGGCTGCT CAGTTCCGCC AGCCCTTAAA GGACCGAAGG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAG CAGCAGAAGC AACAGGTGGC CGAGCAGCAA 
AAGTCGGGCA GCTCTCAGAC AGAGCCCTAT CTGGAGCGTC CCTCCCCAAC GCGCCCTCTT 
CAGCGCCAGA CTACTTGGGC TGGGCGAAGT CTACGGGAAC CATCTTCACC CATGGGGCGC 
CTGGTCAAGA GTGGTAGCCT AGGCAGTGCC CGTGGGACAC AGCCCACGGT GGAGGCTGGT 
GTGGCTCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GCGGATCACC TGTGCCTTGG 
CAGGACGGAA GCCTCTCAGA CCTGAGTCTG ACAGGGGAAG AAATGGCACC TGGAGGCAGC 
CCTGGGGGCT CAGGCTCCGC CCTGAGTGCC CAGTCCACTG AGGCCCTAGA AGGGATAAGT 
GGGCGGGGTT CCAAAACTAG TGGATGTCAG GAGGAGGTGG GCACTCCCAG AAAAGGGCTG 
GGTGCCCGCC TCCAACAGCT GCTCACCCCT TCCCGCCGCG CCTCTGCTTC CCGCATCCCC 
CCACCTGAGT TGCCCTCTGA CCTGCCTCCT GCGGCCCGCC GAAGTCCCAT GGATAGCCTT 
CTGTGGCCCC GAGAACGCCC CGGATCCACT GCTTCTGAGA GCTCTGCTTC TCTGGGCAGT 
GAGTGGGATA TCTCAGAGTC TTCCCTCAGC AGCCTGAGTC TCCGCCGTTC CTCAGAGCGT 
CTCAGTGACA CCCCTGGGGC TTTCCAGCCA CCCTCTCTGG AAATTCTGAT GTCTAGCTGT 
TCCTTGTGTC ATGCCTGTGA TTCCCTGGTG TACGATGAGG AAATCATGGC TGGCTGGGCA 
CCGGATGACT CTAACCTTAA TACAACTTGT CCCTTCTGCG CCTGTCACTT TGTGCCCCTG 
CTCAGTGTCC AGACTCTTGA TTCTCGACCC AGTGCACCTA GTCCAAAGTC TTCCCTTGCT 
GGCGCCAGTG GCTGCAAAGA TGCTCCTGCC CCTGGGGGCC CTGGCCCTGT GCTCAGTGAC 
CGGAGGTTCT GCCTTGCCCT GGATCAGCCC CAGCTCTGCA ATGGGCATAT GGGGAGTGCT 
TCCCGGCGAG TTGAGAATGG GGCATGGGCG TACCTGAGCC CTCTGGTGCT GCGTAAGGAA 
CTGGAGTCCC TGGTAGAGAA TGAGGGCAGC GAGGTGCTGG CGTTACCTGA ACTGCCTGCT 
GCCCACCCTA TCATCTTCTG GAACCTCCTG TGGTATTTCC AACGGCTACG CCTGCCTAGT 
GTTCTACCAG GCCTGGTGTT GGCCTCCTGT AATGGGCCCC CACCCAGCCA GCTCTCCCAG 
GGACCATCTC CATGGCTAAC CCCTGATCCA GCTTCTGTGC ATGTGCATCT GCTGTGGGAT 
GTCCTGACCC CTGATCCCAA CAGCTGCCCA CCTCTCTACG TGCTCTGGAG GGTCCACAGC 
CAGATCCCAC AGCGGGTGGT GTGGCCAGGC CCAGTACCCT CATGTCTTAG CTTGGCATTA 
CTGGAGTCAG TGCTGCGCCA CGTCGGACTC AACGAGGTTC ATAAAGCTGT TGGGCTTCTG 
CTGGAGACGC TAGGGCCCCC TCCCACCGGC CTGCACCTAC AGAGGGGAAT CTACCGTGAG 
ATCTTATTCC TGACCATGGC TGCTCTGGGC AAGGACCACG TGGATATAGT GGCCTTCGAC 
AAGAAGTACA AGTCTGCCTT TAACAAGCTG GCCAGCAGCA TGGGCAAGGA GGAACTGAGG 
CAGCGGCGGG CACAGATGCC CACTCCCAAG GCCATCGACT GCCGAAAGTG TTTTGGAGCA 
CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dennd4b ( XM_017319543.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175032.1 Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X1, mRNA.

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CloneID	OMu80598
Clone ID Related Accession (Same CDS sequence)	XM_017319543.1 , XM_017319543.2
Accession Version	XM_017319543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACAT TATCCTCTCT TGCTCCATCT CTTCCTCGAT TGACCATTCC AGATGCAGTG 
AGTGAAGCAG AGGCCATGGC AGAGGAGCGG CCTCCCCGGT TGGTGGATTA CTTTGTGGTG 
GCTGGGCTTG CAGGAAATGG AGCGCCTATA CCTGAGGAGA AGTGGGTTCC CGAGCCTACC 
GGGCCCCTGC GCCCTCCCCG GCCAGCTGAA CCTATTACAG ATGTGGCAGT CATCGCTAGG 
GCACTGGGCG AGGAGGTGCC CCAGGGCTAT ACATGCATTC AGACTTCTGC GGGTGGCCAC 
CCCTTGGAGC TCAGTGCTGG GCTCCTGGGT GGAACTCAAC CAGTCATCTG CTACCGCAGG 
GGGCGTGACA AGCCCCCCCT CGTTGAGCTG GGGGTGTTAT ATGAAGGGAA GGAACGACCC 
AAGCTTGGGT TCCAAGTGCT TGACACGACA CCGTATAGCC ACTCAGCCAA TCTGGCCCCT 
CCAGGCCCCG GGCACCCCCG CACCTACCTC ATGTATCGGC GGGCAGCAGA AGGGGCAGGG 
CTGCATGCCC TGGGCATCAC TGACCTCTGC CTGGTTCTGC CCAGCAAGGG TGAGGGTACT 
CCTCATACTT ACTGCCGGCT ACCCCGCAAC CTCAACCCTG GCATGTGGGG CCCAGCAGTG 
TACCTGTGCT ACAAGGTAGG CCTGGCCAAG GCCAACACAC TGGTGTATGA GGCAGAGCTG 
CTGGGCCGCT ACCCAGAGGA AGACAATGAG GCGTTTCCGC TGCCCGAGTC AGTCCCTGTC 
TTCTGTCTGC CCATGGGGGC CACTATTGAG TGCTGGCCTG CCCAGACCAA GTACCCAGTA 
CCTGTCTTCT CCACCTTTGT GCTCACAGGC GCTGCTGGTG ATAAGGTGTA TGGTGCTGCT 
TTGCAGTTCT ATGAAGCGTT CCCGAGGGCC AGGCTGTCAG AGAGGCAGGC ACGGGCACTG 
GGCCTGATGA GTGCCGTGGA ACGGGGCCGG GCGCTGGGAG GCCGAGCTGT GCGCAGCCGA 
CGAGCCATAG CTGTGCTGTC CCGCTGGCCT GCCTTCCCAG CCTTCCGAGC CTTCCTCACC 
TTCCTTTACC GATACTCCGT CTCAGGCCCC CATCGTCTGC CCTTGGAAGC GCACATCTCC 
CACTTCATTC ACAACGTCCC ATTCCCATCC CCACAGAGAC CACGCATCCT AGTGCAGATG 
TCTCCCTATG ACAACCTGCT CCTCTGTCAA CCTGTCTCCT CACCCCTGCC CCTCAGTGGT 
GCTAGCTTTT TGCAGCTGCT GCAGAACCTG GGCCCAGAAC TGGCTATTAC ATTGCTGCTG 
GCTGTGCTCA CAGAGCACAA ACTGCTGGTC CACTCGCTGC GGCCAGATCT GCTCACCAGC 
GTGTGCGAGG CCCTTGTCTC AATGATCTTC CCTCTGCATT GGCAGTGTCC CTACATCCCA 
TTGTGCCCGC TAGTGCTGGC GGATGTGCTG AGTGCCCCCG TGCCCTTCAT TGTGGGTATC 
CACTCCAGTT ATTTCGATCT GCATGACCCA CCTGCTGACG TCATCTGTGT TGATCTTGAT 
ACCAACACGC TCTTCCAAAA GGAGGAAAAG AAGCCCCTCT CCGCTAGGAC CCTGCCCCGA 
AGACCATACA AGCTTTTATT GGCCACCCTG ACAAGTCTGT ACCAGCAGCT GGACCAGACT 
TACACTGGAC CAGAGGAAGA GGCCTCCCTG GAATTCCTGC TGACAGACTA TGAGGCTGTG 
TGTGGCCGCA GGACTCGCCT GGAACGGGAA GTCCAGGGAG CCTTCCTGCG CTTCATGGCC 
TGTCTGCTCA AAGGTTACCG GAACTTCCTC CGTCCTCTCA CGCAGGCCCC CTCTGAGGGG 
TCTCGAGATG TTGACAACCT TTTTTACCTT CAGGGTTTCC TCAAGTCCCG GGAACGCTCA 
AGCCACAAGC TGTACTCTCA GCTGCTGCAC ACACAGATGT TCTCACAGTT CATCGAGGAA 
TGCTCTTTTG GCTCCGCCCG GCACGCAGCC CTGGAATTCT TTGACTCTTG TGTTGACAAG 
GTTCACCCAG AGCAGGAGAA GCCCGAACCA ACACCCTTAG TGGAGCTGGA GGAACTATCA 
GGAAGTGAGC TCACTGTCTT TATCACACCC CCAGAGGAAC CACCAGTACT GGAAGGCAGT 
GAGTCTACTC CTCAGTACTG TTATGATGGG TTTCCAGAGC TAAAGGCTGA GCTATTTGAG 
TCTCCTCAGG AACAACAGGG GGCCCTCCCT GTGCCAGGCC CATCCCGGAG TGCTCCCAGC 
AGTCCTGCAC CTCGCCGAAC CAAACAGGAG ATGAAGGTTG CACAGCGAAT GGCACAGAAG 
TCAGCCACTG TGCCTGAGCT GTGGGCCCGG TGCCTGCTGG GTCACTGCTA CGGACTGTGG 
TTCTTATGCC TGCCCGCCTA CGTGCGTTCC GTACCCTCCC GGGTGCGTGC ATTACATACG 
GCCTACCACG TGTTACGGGA AATGGAGAAC CGCAAGGTGG TGCTCCCTGA TGAGGTGTGT 
TACCGCGTGC TGATGCAGCT CTGCTCACAC TATGGGCAGC CCGTTCTGTC TGTGCGAGTC 
ATGCTAGAGA TGCGACGGGC AGGCATTGTG CCCAACACCA TCACTTACGG TTACTACAAC 
AAGGCAGTGC TGGAAAGCAA ATGGCCATCT GGTACTCCGG GTGGACGCCT ACGCTGGGCC 
AAGCTCCGGA ATGTTGTCCT GGGGGCTGCT CAGTTCCGCC AGCCCTTAAA GGACCGAAGG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAG CAGCAGAAGC AACAGGTGGC CGAGCAGCAA 
AAGTCGGGCA GCTCTCAGAC AGAGCCCTAT CTGGAGCGTC CCTCCCCAAC GCGCCCTCTT 
CAGCGCCAGA CTACTTGGGC TGGGCGAAGT CTACGGGAAC CATCTTCACC CATGGGGCGC 
CTGGTCAAGA GTGGTAGCCT AGGCAGTGCC CGTGGGACAC AGCCCACGGT GGAGGCTGGT 
GTGGCTCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GCGGATCACC TGTGCCTTGG 
CAGGACGGAA GCCTCTCAGA CCTGAGTCTG ACAGGGGAAG AAATGGCACC TGGAGGCAGC 
CCTGGGGGCT CAGGCTCCGC CCTGAGTGCC CAGTCCACTG AGGCCCTAGA AGGGATAAGT 
GGGCGGGGTT CCAAAACTAG TGGATGTCAG GAGGAGGTGG GCACTCCCAG AAAAGGGCTG 
GGTGCCCGCC TCCAACAGCT GCTCACCCCT TCCCGCCGCG CCTCTGCTTC CCGCATCCCC 
CCACCTGAGT TGCCCTCTGA CCTGCCTCCT GCGGCCCGCC GAAGTCCCAT GGATAGCCTT 
CTGTGGCCCC GAGAACGCCC CGGATCCACT GCTTCTGAGA GCTCTGCTTC TCTGGGCAGT 
GAGTGGGATA TCTCAGAGTC TTCCCTCAGC AGCCTGAGTC TCCGCCGTTC CTCAGAGCGT 
CTCAGTGACA CCCCTGGGGC TTTCCAGCCA CCCTCTCTGG AAATTCTGAT GTCTAGCTGT 
TCCTTGTGTC ATGCCTGTGA TTCCCTGGTG TACGATGAGG AAATCATGGC TGGCTGGGCA 
CCGGATGACT CTAACCTTAA TACAACTTGT CCCTTCTGCG CCTGTCACTT TGTGCCCCTG 
CTCAGTGTCC AGACTCTTGA TTCTCGACCC AGTGCACCTA GTCCAAAGTC TTCCCTTGCT 
GGCGCCAGTG GCTGCAAAGA TGCTCCTGCC CCTGGGGGCC CTGGCCCTGT GCTCAGTGAC 
CGGAGGTTCT GCCTTGCCCT GGATCAGCCC CAGCTCTGCA ATGGGCATAT GGGGAGTGCT 
TCCCGGCGAG TTGAGAATGG GGCATGGGCG TACCTGAGCC CTCTGGTGCT GCGTAAGGAA 
CTGGAGTCCC TGGTAGAGAA TGAGGGCAGC GAGGTGCTGG CGTTACCTGA ACTGCCTGCT 
GCCCACCCTA TCATCTTCTG GAACCTCCTG TGGTATTTCC AACGGCTACG CCTGCCTAGT 
GTTCTACCAG GCCTGGTGTT GGCCTCCTGT AATGGGCCCC CACCCAGCCA GCTCTCCCAG 
GGACCATCTC CATGGCTAAC CCCTGATCCA GCTTCTGTGC ATGTGCATCT GCTGTGGGAT 
GTCCTGACCC CTGATCCCAA CAGCTGCCCA CCTCTCTACG TGCTCTGGAG GGTCCACAGC 
CAGATCCCAC AGCGGGTGGT GTGGCCAGGC CCAGTACCCT CATGTCTTAG CTTGGCATTA 
CTGGAGTCAG TGCTGCGCCA CGTCGGACTC AACGAGGTTC ATAAAGCTGT TGGGCTTCTG 
CTGGAGACGC TAGGGCCCCC TCCCACCGGC CTGCACCTAC AGAGGGGAAT CTACCGTGAG 
ATCTTATTCC TGACCATGGC TGCTCTGGGC AAGGACCACG TGGATATAGT GGCCTTCGAC 
AAGAAGTACA AGTCTGCCTT TAACAAGCTG GCCAGCAGCA TGGGCAAGGA GGAACTGAGG 
CAGCGGCGGG CACAGATGCC CACTCCCAAG GCCATCGACT GCCGAAAGTG TTTTGGAGCA 
CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175032.1
CDS	667..5241
Translation	MGTLSSLAPSLPRLTIPDAVSEAEAMAEERPPRLVDYFVVAGLAGNGAPIPEEKWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKLGFQVLDTTPYSHSANLAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLMSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQKEEKKPLSARTLPRRPYKLLLATLTSLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTRLEREVQGAFLRFMACLLKGYRNFLRPLTQAPSEGSRDVDNLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTPQYCYDGFPELKAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSATVPELWARCLLGHCYGLWFLCLPAYVRSVPSRVRALHTAYHVLREMENRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLKDRRQQQQQQQQQQQQKQQVAEQQKSGSSQTEPYLERPSPTRPLQRQTTWAGRSLREPSSPMGRLVKSGSLGSARGTQPTVEAGVAHMIEALGVLEPRGSPVPWQDGSLSDLSLTGEEMAPGGSPGGSGSALSAQSTEALEGISGRGSKTSGCQEEVGTPRKGLGARLQQLLTPSRRASASRIPPPELPSDLPPAARRSPMDSLLWPRERPGSTASESSASLGSEWDISESSLSSLSLRRSSERLSDTPGAFQPPSLEILMSSCSLCHACDSLVYDEEIMAGWAPDDSNLNTTCPFCACHFVPLLSVQTLDSRPSAPSPKSSLAGASGCKDAPAPGGPGPVLSDRRFCLALDQPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSVLPGLVLASCNGPPPSQLSQGPSPWLTPDPASVHVHLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPSCLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_017319543.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DENN/MADD domain containing 4B (Dennd4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319543.2

ORF Insert Sequence:

ATGGGAACAT TATCCTCTCT TGCTCCATCT CTTCCTCGAT TGACCATTCC AGATGCAGTG 
AGTGAAGCAG AGGCCATGGC AGAGGAGCGG CCTCCCCGGT TGGTGGATTA CTTTGTGGTG 
GCTGGGCTTG CAGGAAATGG AGCGCCTATA CCTGAGGAGA AGTGGGTTCC CGAGCCTACC 
GGGCCCCTGC GCCCTCCCCG GCCAGCTGAA CCTATTACAG ATGTGGCAGT CATCGCTAGG 
GCACTGGGCG AGGAGGTGCC CCAGGGCTAT ACATGCATTC AGACTTCTGC GGGTGGCCAC 
CCCTTGGAGC TCAGTGCTGG GCTCCTGGGT GGAACTCAAC CAGTCATCTG CTACCGCAGG 
GGGCGTGACA AGCCCCCCCT CGTTGAGCTG GGGGTGTTAT ATGAAGGGAA GGAACGACCC 
AAGCTTGGGT TCCAAGTGCT TGACACGACA CCGTATAGCC ACTCAGCCAA TCTGGCCCCT 
CCAGGCCCCG GGCACCCCCG CACCTACCTC ATGTATCGGC GGGCAGCAGA AGGGGCAGGG 
CTGCATGCCC TGGGCATCAC TGACCTCTGC CTGGTTCTGC CCAGCAAGGG TGAGGGTACT 
CCTCATACTT ACTGCCGGCT ACCCCGCAAC CTCAACCCTG GCATGTGGGG CCCAGCAGTG 
TACCTGTGCT ACAAGGTAGG CCTGGCCAAG GCCAACACAC TGGTGTATGA GGCAGAGCTG 
CTGGGCCGCT ACCCAGAGGA AGACAATGAG GCGTTTCCGC TGCCCGAGTC AGTCCCTGTC 
TTCTGTCTGC CCATGGGGGC CACTATTGAG TGCTGGCCTG CCCAGACCAA GTACCCAGTA 
CCTGTCTTCT CCACCTTTGT GCTCACAGGC GCTGCTGGTG ATAAGGTGTA TGGTGCTGCT 
TTGCAGTTCT ATGAAGCGTT CCCGAGGGCC AGGCTGTCAG AGAGGCAGGC ACGGGCACTG 
GGCCTGATGA GTGCCGTGGA ACGGGGCCGG GCGCTGGGAG GCCGAGCTGT GCGCAGCCGA 
CGAGCCATAG CTGTGCTGTC CCGCTGGCCT GCCTTCCCAG CCTTCCGAGC CTTCCTCACC 
TTCCTTTACC GATACTCCGT CTCAGGCCCC CATCGTCTGC CCTTGGAAGC GCACATCTCC 
CACTTCATTC ACAACGTCCC ATTCCCATCC CCACAGAGAC CACGCATCCT AGTGCAGATG 
TCTCCCTATG ACAACCTGCT CCTCTGTCAA CCTGTCTCCT CACCCCTGCC CCTCAGTGGT 
GCTAGCTTTT TGCAGCTGCT GCAGAACCTG GGCCCAGAAC TGGCTATTAC ATTGCTGCTG 
GCTGTGCTCA CAGAGCACAA ACTGCTGGTC CACTCGCTGC GGCCAGATCT GCTCACCAGC 
GTGTGCGAGG CCCTTGTCTC AATGATCTTC CCTCTGCATT GGCAGTGTCC CTACATCCCA 
TTGTGCCCGC TAGTGCTGGC GGATGTGCTG AGTGCCCCCG TGCCCTTCAT TGTGGGTATC 
CACTCCAGTT ATTTCGATCT GCATGACCCA CCTGCTGACG TCATCTGTGT TGATCTTGAT 
ACCAACACGC TCTTCCAAAA GGAGGAAAAG AAGCCCCTCT CCGCTAGGAC CCTGCCCCGA 
AGACCATACA AGCTTTTATT GGCCACCCTG ACAAGTCTGT ACCAGCAGCT GGACCAGACT 
TACACTGGAC CAGAGGAAGA GGCCTCCCTG GAATTCCTGC TGACAGACTA TGAGGCTGTG 
TGTGGCCGCA GGACTCGCCT GGAACGGGAA GTCCAGGGAG CCTTCCTGCG CTTCATGGCC 
TGTCTGCTCA AAGGTTACCG GAACTTCCTC CGTCCTCTCA CGCAGGCCCC CTCTGAGGGG 
TCTCGAGATG TTGACAACCT TTTTTACCTT CAGGGTTTCC TCAAGTCCCG GGAACGCTCA 
AGCCACAAGC TGTACTCTCA GCTGCTGCAC ACACAGATGT TCTCACAGTT CATCGAGGAA 
TGCTCTTTTG GCTCCGCCCG GCACGCAGCC CTGGAATTCT TTGACTCTTG TGTTGACAAG 
GTTCACCCAG AGCAGGAGAA GCCCGAACCA ACACCCTTAG TGGAGCTGGA GGAACTATCA 
GGAAGTGAGC TCACTGTCTT TATCACACCC CCAGAGGAAC CACCAGTACT GGAAGGCAGT 
GAGTCTACTC CTCAGTACTG TTATGATGGG TTTCCAGAGC TAAAGGCTGA GCTATTTGAG 
TCTCCTCAGG AACAACAGGG GGCCCTCCCT GTGCCAGGCC CATCCCGGAG TGCTCCCAGC 
AGTCCTGCAC CTCGCCGAAC CAAACAGGAG ATGAAGGTTG CACAGCGAAT GGCACAGAAG 
TCAGCCACTG TGCCTGAGCT GTGGGCCCGG TGCCTGCTGG GTCACTGCTA CGGACTGTGG 
TTCTTATGCC TGCCCGCCTA CGTGCGTTCC GTACCCTCCC GGGTGCGTGC ATTACATACG 
GCCTACCACG TGTTACGGGA AATGGAGAAC CGCAAGGTGG TGCTCCCTGA TGAGGTGTGT 
TACCGCGTGC TGATGCAGCT CTGCTCACAC TATGGGCAGC CCGTTCTGTC TGTGCGAGTC 
ATGCTAGAGA TGCGACGGGC AGGCATTGTG CCCAACACCA TCACTTACGG TTACTACAAC 
AAGGCAGTGC TGGAAAGCAA ATGGCCATCT GGTACTCCGG GTGGACGCCT ACGCTGGGCC 
AAGCTCCGGA ATGTTGTCCT GGGGGCTGCT CAGTTCCGCC AGCCCTTAAA GGACCGAAGG 
CAGCAACAGC AGCAGCAGCA GCAGCAGCAG CAGCAGAAGC AACAGGTGGC CGAGCAGCAA 
AAGTCGGGCA GCTCTCAGAC AGAGCCCTAT CTGGAGCGTC CCTCCCCAAC GCGCCCTCTT 
CAGCGCCAGA CTACTTGGGC TGGGCGAAGT CTACGGGAAC CATCTTCACC CATGGGGCGC 
CTGGTCAAGA GTGGTAGCCT AGGCAGTGCC CGTGGGACAC AGCCCACGGT GGAGGCTGGT 
GTGGCTCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GCGGATCACC TGTGCCTTGG 
CAGGACGGAA GCCTCTCAGA CCTGAGTCTG ACAGGGGAAG AAATGGCACC TGGAGGCAGC 
CCTGGGGGCT CAGGCTCCGC CCTGAGTGCC CAGTCCACTG AGGCCCTAGA AGGGATAAGT 
GGGCGGGGTT CCAAAACTAG TGGATGTCAG GAGGAGGTGG GCACTCCCAG AAAAGGGCTG 
GGTGCCCGCC TCCAACAGCT GCTCACCCCT TCCCGCCGCG CCTCTGCTTC CCGCATCCCC 
CCACCTGAGT TGCCCTCTGA CCTGCCTCCT GCGGCCCGCC GAAGTCCCAT GGATAGCCTT 
CTGTGGCCCC GAGAACGCCC CGGATCCACT GCTTCTGAGA GCTCTGCTTC TCTGGGCAGT 
GAGTGGGATA TCTCAGAGTC TTCCCTCAGC AGCCTGAGTC TCCGCCGTTC CTCAGAGCGT 
CTCAGTGACA CCCCTGGGGC TTTCCAGCCA CCCTCTCTGG AAATTCTGAT GTCTAGCTGT 
TCCTTGTGTC ATGCCTGTGA TTCCCTGGTG TACGATGAGG AAATCATGGC TGGCTGGGCA 
CCGGATGACT CTAACCTTAA TACAACTTGT CCCTTCTGCG CCTGTCACTT TGTGCCCCTG 
CTCAGTGTCC AGACTCTTGA TTCTCGACCC AGTGCACCTA GTCCAAAGTC TTCCCTTGCT 
GGCGCCAGTG GCTGCAAAGA TGCTCCTGCC CCTGGGGGCC CTGGCCCTGT GCTCAGTGAC 
CGGAGGTTCT GCCTTGCCCT GGATCAGCCC CAGCTCTGCA ATGGGCATAT GGGGAGTGCT 
TCCCGGCGAG TTGAGAATGG GGCATGGGCG TACCTGAGCC CTCTGGTGCT GCGTAAGGAA 
CTGGAGTCCC TGGTAGAGAA TGAGGGCAGC GAGGTGCTGG CGTTACCTGA ACTGCCTGCT 
GCCCACCCTA TCATCTTCTG GAACCTCCTG TGGTATTTCC AACGGCTACG CCTGCCTAGT 
GTTCTACCAG GCCTGGTGTT GGCCTCCTGT AATGGGCCCC CACCCAGCCA GCTCTCCCAG 
GGACCATCTC CATGGCTAAC CCCTGATCCA GCTTCTGTGC ATGTGCATCT GCTGTGGGAT 
GTCCTGACCC CTGATCCCAA CAGCTGCCCA CCTCTCTACG TGCTCTGGAG GGTCCACAGC 
CAGATCCCAC AGCGGGTGGT GTGGCCAGGC CCAGTACCCT CATGTCTTAG CTTGGCATTA 
CTGGAGTCAG TGCTGCGCCA CGTCGGACTC AACGAGGTTC ATAAAGCTGT TGGGCTTCTG 
CTGGAGACGC TAGGGCCCCC TCCCACCGGC CTGCACCTAC AGAGGGGAAT CTACCGTGAG 
ATCTTATTCC TGACCATGGC TGCTCTGGGC AAGGACCACG TGGATATAGT GGCCTTCGAC 
AAGAAGTACA AGTCTGCCTT TAACAAGCTG GCCAGCAGCA TGGGCAAGGA GGAACTGAGG 
CAGCGGCGGG CACAGATGCC CACTCCCAAG GCCATCGACT GCCGAAAGTG TTTTGGAGCA 
CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DENN domain proteins: regulators of Rab GTPases.
The Journal of biological chemistry286(16)13791-800(2011 Apr)
Marat AL,Dokainish H,McPherson PS

Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors.
The Journal of cell biology191(2)367-81(2010 Oct)
Yoshimura S,Gerondopoulos A,Linford A,Rigden DJ,Barr FA

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Dennd4b cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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