Piezo1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Piezo1
Entrez Gene ID	361430
Full Name	piezo-type mechanosensitive ion channel component 1
Synonyms	Fam38a,Mib
General protein information	Preferred Names piezo-type mechanosensitive ion channel component 1 Names piezo-type mechanosensitive ion channel component 1 family with sequence similarity 38, member A membrane protein induced by beta-amyloid treatment protein PIEZO1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601325.1	XP_017456814.1	piezo-type mechanosensitive ion channel component 1 isoform X3
XM_008772626.2	XP_008770848.1	piezo-type mechanosensitive ion channel component 1 isoform X1
XM_008772627.2	XP_008770849.1	piezo-type mechanosensitive ion channel component 1 isoform X2
NM_001077200.2	NP_001070668.2	piezo-type mechanosensitive ion channel component 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	FAM38A	XP_001099984.2
Bos taurus (cattle)	PIEZO1	XP_002694905.2
Gallus gallus (chicken)	FAM38A	XP_414209.4
Homo sapiens (human)	PIEZO1	NP_001136336.2
Rattus norvegicus (Norway rat)	Piezo1	NP_001070668.2
Mus musculus (house mouse)	Piezo1	NP_001032375.1
Xenopus tropicalis (tropical clawed frog)	piezo1	XP_002933721.2
Danio rerio (zebrafish)	piezo1	XP_696355.4
Pan troglodytes (chimpanzee)	PIEZO1	XP_003952965.1
Canis lupus familiaris (dog)	PIEZO1	XP_005620632.1

Gene Ontology
Bibliography

Related articles in PubMed

The Piezo1 cation channel mediates uterine artery shear stress mechanotransduction and vasodilation during rat pregnancy.
John L, Ko NL, Gokin A, Gokina N, Mandalà M, Osol G
American journal of physiology. Heart and circulatory physiology315(4)H1019-H1026(2018 10)

Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis.
Liu Q, Sun B, Zhao J, Wang Q, An F, Hu X, Yang Z, Xu J, Tan M, Li L
Experimental & molecular medicine50(5)60(2018 05)

Structure and mechanogating mechanism of the Piezo1 channel.
Zhao Q, Zhou H, Chi S, Wang Y, Wang J, Geng J, Wu K, Liu W, Zhang T, Dong MQ, Wang J, Li X, Xiao B
Nature554(7693)487-492(2018 02)

Structure of the mechanically activated ion channel Piezo1.
Saotome K, Murthy SE, Kefauver JM, Whitwam T, Patapoutian A, Ward AB
Nature554(7693)481-486(2018 02)

Piezo1 expression increases in rat bladder after partial bladder outlet obstruction.
Michishita M, Yano K, Tomita KI, Matsuzaki O, Kasahara KI
Life sciences1661-7(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Piezo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Piezo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa39814	NM_001077200.2 Latest version!	Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ORa47912	XM_008772626.2 Latest version!	Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ORa47913	XM_008772627.2 Latest version!	Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ORa47914	XM_017601325.1 Latest version!	Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Piezo1 ( NM_001077200.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001070668.2 Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), mRNA.

Price & Availability↑

CloneID	ORa39814
Clone ID Related Accession (Same CDS sequence)	NM_001077200.2
Accession Version	NM_001077200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570636800000
Organism	Rattus norvegicus(Norway rat)
Product	piezo-type mechanosensitive ion channel component 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC134009.3, AB161229.1 and BQ196181.1. On Sep 3, 2009 this sequence version replaced NM_001077200.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG 
CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT 
CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC 
CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGTAACCT TTGGGCAAAC 
GTGTCTCAAC ACATAGGGGT TACAAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGA 
CTGGTGGCAC CTGACCTGGG AGTGCTGGTG GCATCTTCCC TTTGCCTTGG CCTCTGTGGA 
CGCCTCACAA GGAAAGCCCG GCAGAGCCAG CGCACTCAGG AGCTGGAGGA GGATGACATA 
GATGCTGCCC CCGCCGCAGG GCTGCAGGGA GCCCCCACCC TGGCAACCAA ACGCAGGCTG 
TGGCTGGCCC CTCGCTTCCG GATCACGGCG CACTGGCTGC TGGTGACCTC CGGACGGATG 
CTGGTCATCG TGCTACTGGC ACTCGCAGGC ATAGCCCATC CTTCGGCCTT CTCCAGCGTC 
TACCTGATGG TGTTCCTGGC CATCTGCACC TGGTGGTCTT GCCACTTTCC TCTCAGCTCC 
CTGGGCTTTA ACACCCTCTG CGTCATGGTG AGCTGCTTTG GCGCCGGCCA TCTTGTTTGT 
TTATACTGCT ATCAGACACC ATTTGTCCAG AGCGTGCTAC TTCCTGGCAG CCTCTGGGCC 
AGGCTATTTG GTCTCAAGAA CTTCGTAGAC ATCCCTAACT GCTCCAGCCC CAACGTGCTG 
GTACTCAACA CTAAGCATGC TTGGCCCATC TACGTGAGCC CCGGTATCCT GCTGCTGTTG 
TACTACACAG CCACCTCTCT CCTGAAGCTC CGCAAGGGCC GCTTCTCAGA GCTGAGGAAG 
GAGATCCCGA GGGAGGATGA GGAGCATGAA CTGGAACTGG ACCAGCTGGA GCCAGAGCCC 
CAGGCTAGGG GCACCACCCA GGGTGCGACG CCCACGACCA CTGGACCCGA CATTGACAAC 
TGCACCGTGC ATGTACTAAC CAGCCAGAGC CCCGTCCGCC AGCGTCCTGT ACGCCCCAGG 
CTGGCTGAGC TGAAAGAGAT GTCACCACTA CATGGCCTGG GCCACCTCAT CCTGGACCAG 
AGCTATGTGT GTGCTCTGAT CGCCATGATG GTGTGGAGCA TCATGTACCA CAGCTGGCTG 
ACCTTCGTCT TGCTGCTCTG GGCCTGCCTC ATCTGGACCG TGCGCAGCCG CCACCAGTTG 
GCTATGCTGT GCTCACCCTG CATCCTGCTG TACGGACTGA CACTCTGCTG CCTGCGGTAC 
GTGTGGGCCA TGGAGCTCCC CGAGCTGCCC ACCACCCTGG GCCCTGTCAG TCTACATCAG 
TTGGGATTGG AGCATACACG CTACCCCTGC CTGGACCTTG GTGCCATGCT GCTGTATCTG 
CTCACGTTCT GGCTGCTCCT GCGTCAGTTC GTGAAGGAGA AGCTGCTGAA GAAGAGAAAG 
GCACCTTCGA CGCTGCTGGA GGTCACAGTG TCCGACACAG AGCCCACACA GACCCAGACA 
CTGCTGAGGA GCCTGGGGGA GCTGGTCACG GGCATCTACG TCAAGTACTG GATCTACGTG 
TGCGCTGGGA TGTTCATTGT GGTCAGCTTC GCTGGCCGCC TGGTGGTCTA CAAAATCGTC 
TACATGTTCC TTTTCCTGCT GTGCCTCACG TTGTTCCAGG TCTACTACAC TCTGTGGAGG 
AAGCTGCTCC GAGTTTTCTG GTGGCTCGTG GTGGCCTACA CCATGCTCGT GCTCATCGCC 
GTGTACACTT TCCAGTTCCA GGACTTCCCC ACCTACTGGC GCAACCTCAC GGGCTTCACA 
GATGAACAGT TGGGCGACCT GGGCCTGGAG CAGTTCAGCG TGTCGGAGCT CTTTTCCAGC 
ATCCTCATCC CTGGCTTCTT CCTGCTGGCC TGCATCCTGC AGCTGCACTA CTTCCACAGG 
CCGTTCATGC AGCTCACCGA CCTGGAGCAC GTGCCCCCGC CAGGCACACG CCGCCTTCGC 
TGGGCTCACA GGCAGGATAC AGTGAGCGAG GCCCCTCTGC TTCAGCATCA GGAGGAGGAG 
GAAGTCTTCA GGGATGACGG GCAGAGCATG GATGGGCCCC ACCAGACCAC ACAGGTCCCT 
GAGGGTACAG CCAGCAAGTG GGGCCTGGTG GCCGACCGGC TGCTGGACCT AGCGTCCAGC 
TTCTCCGCCG TCCTCACCCG CATCCAGGTG TTCGTACGAT GTTTGCTCGA GCTTCACGTC 
TTCAAGCTGG TGGCTCTCTA CACTGTCTGG GTGGCCCTGA AAGAAGTGTC CGTGATGAAC 
CTGCTGCTGG TGGTGCTGTG GGCCTTCGCC CTGCCCTACC CGCGCTTCCG GCCCATGGCC 
TCCTGCCTGT CCACCGTGTG GACCTGTATC ATCATCGTGT GCAAGATGCT GTATCAGCTG 
AAGATTGTCA ACCCACACGA GTACTCCAGT AACTGCACCG AGCCCTTTCC CAACAATACC 
AACTTGCAGC CCTTGGAGAT CAGCCAGTCT CTGCTGTACC GTGGCCCCGT TGACCCTGCC 
AACTGGTTTG GGGTGCGGAA GGGTTACCCA AACTTGGGCT ATATCCAGAA CCACCTGCAG 
ATCCTTCTGC TGCTGGTGTT TGAGGCTGTG GTGTACCGGC GCCAAGAGCA CTACCGCCGG 
CAGCACCAGC AGGCCCCTCT GCCCGCCCAG GCTTTGTGCG CTGATGGCAC CCGCCAGAGG 
CTGGACCAGG ACCTACTCAG CTGCCTCAAA TATTTCATCA ACTTCTTCTT CTACAAATTC 
GGGCTGGAGA TCTGCTTCTT GATGGCCGTG AATGTCATCG GACAACGCAT GAACTTCATG 
GTGATCTTAC ACGGCTGCTG GTTGGTGGCC ATCCTTACAC GCCGGCGCCG TGAGGCCATC 
GCCCGCCTCT GGCCCAATTA CTGTCTGTTC CTCACGCTGT TCCTGCTGTA CCAGTACCTG 
CTGTGCCTGG GCATGCCTCC CGCACTGTGC ATCGACTACC CGTGGCGCTG GAGCCAGGCC 
ATCCCCATGA ATTCTGCGCT CATCAAGTGG CTGTACCTCC CTGACTTCTT CAGGGCCCCC 
AACTCCACCA ACCTTATCAG TGACTTCCTC CTGCTACTCT GCGCCTCCCA ACAGTGGCAG 
GTGTTCTCCG CAGAGCAAAC GGAAGAATGG CAGCGCATGG CTGGCGTTAA CACTGACCAC 
CTGGAGCCCC TGCGTGGGGA GCCCAACCCT ATCCCCAACT TCATCCACTG CAGGTCCTAT 
CTGGATATGC TGAAGGTGGC CGTCTTCCGC TACCTGTTCT GGCTGGTGCT GGTGGTCGTG 
TTTGTCACAG GGGCCACCCG CATCAGCATC TTTGGGCTGG GGTACCTGCT GGCCTGCTTC 
TACCTGCTGC TCTTTGGCAC CACCCTGCTG CAGAAGGACA CACGAGCCCA GCTCGTGCTG 
TGGGACTGCC TCATCCTCTA CAACGTCACC GTCATCATCT CTAAGAATAT GTTGTCGCTC 
CTGTCCTGTG TCTTCGTGGA GCAAATGCAG AGCAACTTCT GCTGGGTCAT CCAGCTCTTC 
AGCCTCGTGT GCACAGTCAA AGGCTACTAT GACCCCAAAG AGATGAAGAC CAGGGACCGG 
GATTGCCTGC TGCCTGTGGA GGAGGCCGGG ATCATCTGGG ACAGCATCTG CTTCTTCTTC 
CTGCTCCTAC AACGGCGCGT CTTTCTCAGC CACTACTTCC TGCACGTCAG TGCTGACCTG 
AAGGCCACGG CCCTGCAGGC CTCCAGGGGT TTTGCTCTCT ACAATGCGGC CAACATAAAG 
AACATCAACT TCCACCGTCA GACCGAGGAG AGGTCCCTGG CCCAGCTGAA AAGACAGATG 
AAGCGCATCC GTGCCAAACA GGAGAAATAC CGGCAGAGCC AGGCAAGCCG TGGTCAACTC 
CAGTCCACAG ACCCTCAGGA GCCAGGGCCT GACAGCCCAG GGGGTTCCTC CCCGCCACGG 
ACACAGTGGT GGCGCCCCTG GCAGGACCAC GCCACAGTCA TCCACTCTGG TGACTACTTC 
CTGTTTGAGT CGGACAGTGA GGAGGAAGAG GAGGCCCTGC CTGAGGACCC CAGGCCTGCA 
GCTCAGAGTG CCTTCCAGAT GGCATACCAG GCATGGGTAA CCAACGCCCA GACAGTGCTA 
AGGCAGCGTC GTGAACAGGC ACGGCGGGAT CGGGCAGAAC AGCTGGCTTC TGGAGGCGAC 
TTGAGTCCTG AGGTGGAGCT GGTAGACGTC CCAGAGAATG AGATGGCAGG CCATAGCCAT 
GTGATGCAGC GCGTGCTGAG TACCATGCAG TTCCTGTGGG TGCTGGGCCA GGCCACAGTG 
GACGGGCTGA CGCGCTGGTT GCGTACGTTC ACGAAGGACC ACCGCACCAT GAGTGATGTG 
CTGTGTGCAG AGCGTTACCT GCTCACCCAG GAGCTTCTTC GGGGTGGAGA GGTACACCGA 
GGGGTACTGG ACCAGCTTTA CGTGAGCGAA GATGAGATCG CACTGTCAGG TCCTGTGGAG 
AACCGTGATG GACCCAGCAC AGCCTCTAGC GGGCTGGGAG CTGAAGAGCC TTTGAGTAGC 
ATGACAGACG ACACCGGCAG CCCCCTTAGC ACTGGCTACA ACACTCGCAG TGGTAGTGAG 
GAGATTATCA CCGACACTGG GGGCCTCCAG GCTGGGACCT CCCTGCACGG CTCCCAAGAG 
CTTCTAGCCA ATGCTCGTAC CCGGATGCGC ACAGCCAGTG AGCTGCTGCT GGACAGGCGC 
CTGCGTATCC CTGAGCTGGA GGAGGCCGAG CAGTTTGAGG CACAGCAGGG CCGGACGCTG 
CGGCTGCTCA GAGCCATGTA CCAGTGTGTG GCGGCCCACT CAGAGCTTCT CTGTTACTTC 
ATCATCATCC TTAACCACAT GGTGACAGCC TCGGCTGCCT CCCTGGTGCT GCCTGTGCTT 
GTGTTCCTCT GGGCCATGCT GACCATCCCG AGGCCTAGCA AGCGCTTCTG GATGACAGCT 
ATCGTCTTCA CTGAGGTCAT GGTGGTCACC AAATACTTGT TCCAGTTTGG CTTCTTCCCC 
TGGAACAGCT ACATAGTGCT TCGACGCTAC GAGAACAAGC CCTACTTCCC TCCGCGCATC 
CTGGGCCTCG AGAAAACAGA CAGCTATATC AAGTACGACC TGGTGCAACT CATGGCCCTC 
TTCTTCCACC GCTCGCAGTT ACTGTGCTAT GGCCTCTGGG ACCATGAGGA GGATGGTGTC 
CCCAAGGACC ATTGCAGGAG TAGCGAAAAG GACCAGGAAG CTGAGGAAGA GTCAGAGGCT 
AAGCTGGAAT CGCAGCCTGA GACGGGCACT GGGCATCCTG AGGAGCCCGT GTTGACTGGT 
ACCCCCAAGG ACCACATCCA AGGGAAAGGA AGTGTTAGGT CCAAGGATGA GATCCAAGAT 
CCCCCAGAGG ACCTTAAGCC CCAGCACAGG AGACACATCA GCATACGCTT CAGGAGGAGG 
AAGGAGACTC AAGGACCCAA AGGAGCAGCA GTCGTGGAGG CTGAGCACGA GGAGGGAGAG 
GAAGGAAGAG AAGCTGCAGG GAGAAAGAGG CTGCGTCGCC CTCGAGAAGG GTTGAAGATT 
CGGGAGAAAA TGAAGGCAGC TGGACGCCGG CTGCAGAGCT TCTGTCTGTC ACTGGCCCAG 
AGCTTTTACC AACCCCTGCG GCGTTTCTTC CATGACATTC TGCACACCAA GTACCGAGCG 
GCCACCGACG TCTATGCCCT CATGTTCCTG GCTGACATTG TCGACATTGT CGTCATCATC 
TTCGGTTTTT GGGCTTTTGG GAAGCACTCC GCGGCCACGG ACATTGCATC CTCACTGTCA 
GATGACCAGG TGCCACAGGC CTTCCTGTTC ATGCTGCTGG TCCAGTTCGG CACCATGGTC 
ATAGACCGCG CCCTCTACCT GCGTAAGACT GTACTGGGCA ACCTGGCCTT TCAGGTGGTC 
CTGGTGGTGG CCATTCACCT CTGGATGTTT TTCATCTTAC CGGCTGTCAC CGAGAGGATG 
TTCAGACAGA ACGCGGTGGC CCAGCTGTGG TACTTCGTGA AGTGCATTTA CTTTGCCCTG 
TCCGCCTACC AGATCCGCTG TGGCTACCCC ACCCGTATCT TGGGCAACTT CCTCACCAAG 
AAGTACAATC ATCTAAACCT CTTCCTTTTC CAGGGGTTCC GTCTAGTGCC ATTCCTGGTG 
GAGCTGCGGG CCGTCATGGA CTGGGTATGG ACTGACACCA CGCTGTCCCT GTCCAACTGG 
ATGTGTGTGG AAGACATCTA TGCCAACATC TTTATCATCA AGTGCAGCCG AGAGACAGAG 
AAGAAATACC CCCAGCCCAA GGGACAGAAG AAGAAGAAAA TCGTCAAGTA TGGCATGGGA 
GGTCTCATCA TCCTCTTCCT CATTGCCATC ATCTGGTTCC CTCTGCTCTT CATGTCACTG 
ATCCGCTCTG TGGTTGGTGT CGTCAACCAG CCCATTGATG TCACTGTTAC CCTCAAGTTA 
GGTGGCTACG AGCCACTGTT CACCATGAGC GCCCAGCAGC CATCCATAGT GCCATTCACT 
CCCGAGGACT ATGAGGAGCT GTCCCAGCAG TTTGACCCCT ACCCACTAGC CATGCAGTTC 
ATTAGCCAGT ACAGTCCTGA GGACATCGTC ACTGCACAGA TTGAGGGCAG CTCGGGGGCC 
CTGTGGCGCA TCAGCCCGCC CAGCCGGGCC CAGATGAAGC ACGAGCTGTA CAACGGCACA 
GCCGACATCA CACTGCGCTT CACCTGGAAT TTCCAAAGGG ACCTGGCCAA GGGCGGCTCT 
GTGGAGTATA CCAACGAGAA GCACACCTTG GAGCTGGCCC CCAACAGTAC GGCACGAAGG 
CAGCTGGCCC AACTGCTCGA GGGCAGACCT GACCAGTCAG TGGTCATTCC CCACCTCTTC 
CCCAAGTACA TTCGTGCACC CAATGGGCCT GAAGCCAATC CTGTGAAGCA ACTGCAGCCA 
GACGAGGAAG AGGACTACCT TGGTGTGCGT ATCCAGCTGC GGAGAGAGCA AGTGGGCACA 
GGAACCTCTG GGGAGCAAGC AGGCACAAAG GCCTCCGACT TCCTCGAGTG GTGGGTGATT 
GAGCTGCAGG ACTGCCAGGC CGAGTGCAAC CTGCTGCCCA TGGTCATCTT CAGTGACAAG 
GTCAGCCCGC CTAGCCTGGG CTTCCTGGCC GGCTACGGGA TTGTGGGGCT CTACGTCTCT 
ATCGTGCTGG TCGTTGGCAA GTTTGTGCGG GGCTTCTTCA GTGATATCTC ACACTCCATC 
ATGTTCGAGG AGCTGCCGTG CGTGGACCGC ATCCTCAAGC TGTGCCAGGA CATCTTCTTG 
GTACGGGAGA CCCGCGAGCT GGAGCTGGAG GAGGAACTCT ATGCCAAGCT CATATTCCTG 
TACCGATCAC CAGAGACCAT GATCAAGTGG ACGCGTGAGA AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070668.2
CDS	291..7898
Translation	MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWLPGPSRHSIPGHTGRLLRALLCLSLLFLVAHVAFQICLHTMPRLNQLLGQNCNLWANVSQHIGVTRLDLKDIFNTTRLVAPDLGVLVASSLCLGLCGRLTRKARQSQRTQELEEDDIDAAPAAGLQGAPTLATKRRLWLAPRFRITAHWLLVTSGRMLVIVLLALAGIAHPSAFSSVYLMVFLAICTWWSCHFPLSSLGFNTLCVMVSCFGAGHLVCLYCYQTPFVQSVLLPGSLWARLFGLKNFVDIPNCSSPNVLVLNTKHAWPIYVSPGILLLLYYTATSLLKLRKGRFSELRKEIPREDEEHELELDQLEPEPQARGTTQGATPTTTGPDIDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLHGLGHLILDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFVKEKLLKKRKAPSTLLEVTVSDTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPTYWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHVPPPGTRRLRWAHRQDTVSEAPLLQHQEEEEVFRDDGQSMDGPHQTTQVPEGTASKWGLVADRLLDLASSFSAVLTRIQVFVRCLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEISQSLLYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQALCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSQAIPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAEQTEEWQRMAGVNTDHLEPLRGEPNPIPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTTLLQKDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYDPKEMKTRDRDCLLPVEEAGIIWDSICFFFLLLQRRVFLSHYFLHVSADLKATALQASRGFALYNAANIKNINFHRQTEERSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSTDPQEPGPDSPGGSSPPRTQWWRPWQDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQMAYQAWVTNAQTVLRQRREQARRDRAEQLASGGDLSPEVELVDVPENEMAGHSHVMQRVLSTMQFLWVLGQATVDGLTRWLRTFTKDHRTMSDVLCAERYLLTQELLRGGEVHRGVLDQLYVSEDEIALSGPVENRDGPSTASSGLGAEEPLSSMTDDTGSPLSTGYNTRSGSEEIITDTGGLQAGTSLHGSQELLANARTRMRTASELLLDRRLRIPELEEAEQFEAQQGRTLRLLRAMYQCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVMVVTKYLFQFGFFPWNSYIVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQLLCYGLWDHEEDGVPKDHCRSSEKDQEAEEESEAKLESQPETGTGHPEEPVLTGTPKDHIQGKGSVRSKDEIQDPPEDLKPQHRRHISIRFRRRKETQGPKGAAVVEAEHEEGEEGREAAGRKRLRRPREGLKIREKMKAAGRRLQSFCLSLAQSFYQPLRRFFHDILHTKYRAATDVYALMFLADIVDIVVIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLRKTVLGNLAFQVVLVVAIHLWMFFILPAVTERMFRQNAVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPEDYEELSQQFDPYPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKHELYNGTADITLRFTWNFQRDLAKGGSVEYTNEKHTLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLGVRIQLRREQVGTGTSGEQAGTKASDFLEWWVIELQDCQAECNLLPMVIFSDKVSPPSLGFLAGYGIVGLYVSIVLVVGKFVRGFFSDISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	NM_001077200.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077200.2

ORF Insert Sequence:

ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG 
CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT 
CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC 
CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGTAACCT TTGGGCAAAC 
GTGTCTCAAC ACATAGGGGT TACAAGGCTG GACCTGAAGG ACATCTTTAA CACCACCAGA 
CTGGTGGCAC CTGACCTGGG AGTGCTGGTG GCATCTTCCC TTTGCCTTGG CCTCTGTGGA 
CGCCTCACAA GGAAAGCCCG GCAGAGCCAG CGCACTCAGG AGCTGGAGGA GGATGACATA 
GATGCTGCCC CCGCCGCAGG GCTGCAGGGA GCCCCCACCC TGGCAACCAA ACGCAGGCTG 
TGGCTGGCCC CTCGCTTCCG GATCACGGCG CACTGGCTGC TGGTGACCTC CGGACGGATG 
CTGGTCATCG TGCTACTGGC ACTCGCAGGC ATAGCCCATC CTTCGGCCTT CTCCAGCGTC 
TACCTGATGG TGTTCCTGGC CATCTGCACC TGGTGGTCTT GCCACTTTCC TCTCAGCTCC 
CTGGGCTTTA ACACCCTCTG CGTCATGGTG AGCTGCTTTG GCGCCGGCCA TCTTGTTTGT 
TTATACTGCT ATCAGACACC ATTTGTCCAG AGCGTGCTAC TTCCTGGCAG CCTCTGGGCC 
AGGCTATTTG GTCTCAAGAA CTTCGTAGAC ATCCCTAACT GCTCCAGCCC CAACGTGCTG 
GTACTCAACA CTAAGCATGC TTGGCCCATC TACGTGAGCC CCGGTATCCT GCTGCTGTTG 
TACTACACAG CCACCTCTCT CCTGAAGCTC CGCAAGGGCC GCTTCTCAGA GCTGAGGAAG 
GAGATCCCGA GGGAGGATGA GGAGCATGAA CTGGAACTGG ACCAGCTGGA GCCAGAGCCC 
CAGGCTAGGG GCACCACCCA GGGTGCGACG CCCACGACCA CTGGACCCGA CATTGACAAC 
TGCACCGTGC ATGTACTAAC CAGCCAGAGC CCCGTCCGCC AGCGTCCTGT ACGCCCCAGG 
CTGGCTGAGC TGAAAGAGAT GTCACCACTA CATGGCCTGG GCCACCTCAT CCTGGACCAG 
AGCTATGTGT GTGCTCTGAT CGCCATGATG GTGTGGAGCA TCATGTACCA CAGCTGGCTG 
ACCTTCGTCT TGCTGCTCTG GGCCTGCCTC ATCTGGACCG TGCGCAGCCG CCACCAGTTG 
GCTATGCTGT GCTCACCCTG CATCCTGCTG TACGGACTGA CACTCTGCTG CCTGCGGTAC 
GTGTGGGCCA TGGAGCTCCC CGAGCTGCCC ACCACCCTGG GCCCTGTCAG TCTACATCAG 
TTGGGATTGG AGCATACACG CTACCCCTGC CTGGACCTTG GTGCCATGCT GCTGTATCTG 
CTCACGTTCT GGCTGCTCCT GCGTCAGTTC GTGAAGGAGA AGCTGCTGAA GAAGAGAAAG 
GCACCTTCGA CGCTGCTGGA GGTCACAGTG TCCGACACAG AGCCCACACA GACCCAGACA 
CTGCTGAGGA GCCTGGGGGA GCTGGTCACG GGCATCTACG TCAAGTACTG GATCTACGTG 
TGCGCTGGGA TGTTCATTGT GGTCAGCTTC GCTGGCCGCC TGGTGGTCTA CAAAATCGTC 
TACATGTTCC TTTTCCTGCT GTGCCTCACG TTGTTCCAGG TCTACTACAC TCTGTGGAGG 
AAGCTGCTCC GAGTTTTCTG GTGGCTCGTG GTGGCCTACA CCATGCTCGT GCTCATCGCC 
GTGTACACTT TCCAGTTCCA GGACTTCCCC ACCTACTGGC GCAACCTCAC GGGCTTCACA 
GATGAACAGT TGGGCGACCT GGGCCTGGAG CAGTTCAGCG TGTCGGAGCT CTTTTCCAGC 
ATCCTCATCC CTGGCTTCTT CCTGCTGGCC TGCATCCTGC AGCTGCACTA CTTCCACAGG 
CCGTTCATGC AGCTCACCGA CCTGGAGCAC GTGCCCCCGC CAGGCACACG CCGCCTTCGC 
TGGGCTCACA GGCAGGATAC AGTGAGCGAG GCCCCTCTGC TTCAGCATCA GGAGGAGGAG 
GAAGTCTTCA GGGATGACGG GCAGAGCATG GATGGGCCCC ACCAGACCAC ACAGGTCCCT 
GAGGGTACAG CCAGCAAGTG GGGCCTGGTG GCCGACCGGC TGCTGGACCT AGCGTCCAGC 
TTCTCCGCCG TCCTCACCCG CATCCAGGTG TTCGTACGAT GTTTGCTCGA GCTTCACGTC 
TTCAAGCTGG TGGCTCTCTA CACTGTCTGG GTGGCCCTGA AAGAAGTGTC CGTGATGAAC 
CTGCTGCTGG TGGTGCTGTG GGCCTTCGCC CTGCCCTACC CGCGCTTCCG GCCCATGGCC 
TCCTGCCTGT CCACCGTGTG GACCTGTATC ATCATCGTGT GCAAGATGCT GTATCAGCTG 
AAGATTGTCA ACCCACACGA GTACTCCAGT AACTGCACCG AGCCCTTTCC CAACAATACC 
AACTTGCAGC CCTTGGAGAT CAGCCAGTCT CTGCTGTACC GTGGCCCCGT TGACCCTGCC 
AACTGGTTTG GGGTGCGGAA GGGTTACCCA AACTTGGGCT ATATCCAGAA CCACCTGCAG 
ATCCTTCTGC TGCTGGTGTT TGAGGCTGTG GTGTACCGGC GCCAAGAGCA CTACCGCCGG 
CAGCACCAGC AGGCCCCTCT GCCCGCCCAG GCTTTGTGCG CTGATGGCAC CCGCCAGAGG 
CTGGACCAGG ACCTACTCAG CTGCCTCAAA TATTTCATCA ACTTCTTCTT CTACAAATTC 
GGGCTGGAGA TCTGCTTCTT GATGGCCGTG AATGTCATCG GACAACGCAT GAACTTCATG 
GTGATCTTAC ACGGCTGCTG GTTGGTGGCC ATCCTTACAC GCCGGCGCCG TGAGGCCATC 
GCCCGCCTCT GGCCCAATTA CTGTCTGTTC CTCACGCTGT TCCTGCTGTA CCAGTACCTG 
CTGTGCCTGG GCATGCCTCC CGCACTGTGC ATCGACTACC CGTGGCGCTG GAGCCAGGCC 
ATCCCCATGA ATTCTGCGCT CATCAAGTGG CTGTACCTCC CTGACTTCTT CAGGGCCCCC 
AACTCCACCA ACCTTATCAG TGACTTCCTC CTGCTACTCT GCGCCTCCCA ACAGTGGCAG 
GTGTTCTCCG CAGAGCAAAC GGAAGAATGG CAGCGCATGG CTGGCGTTAA CACTGACCAC 
CTGGAGCCCC TGCGTGGGGA GCCCAACCCT ATCCCCAACT TCATCCACTG CAGGTCCTAT 
CTGGATATGC TGAAGGTGGC CGTCTTCCGC TACCTGTTCT GGCTGGTGCT GGTGGTCGTG 
TTTGTCACAG GGGCCACCCG CATCAGCATC TTTGGGCTGG GGTACCTGCT GGCCTGCTTC 
TACCTGCTGC TCTTTGGCAC CACCCTGCTG CAGAAGGACA CACGAGCCCA GCTCGTGCTG 
TGGGACTGCC TCATCCTCTA CAACGTCACC GTCATCATCT CTAAGAATAT GTTGTCGCTC 
CTGTCCTGTG TCTTCGTGGA GCAAATGCAG AGCAACTTCT GCTGGGTCAT CCAGCTCTTC 
AGCCTCGTGT GCACAGTCAA AGGCTACTAT GACCCCAAAG AGATGAAGAC CAGGGACCGG 
GATTGCCTGC TGCCTGTGGA GGAGGCCGGG ATCATCTGGG ACAGCATCTG CTTCTTCTTC 
CTGCTCCTAC AACGGCGCGT CTTTCTCAGC CACTACTTCC TGCACGTCAG TGCTGACCTG 
AAGGCCACGG CCCTGCAGGC CTCCAGGGGT TTTGCTCTCT ACAATGCGGC CAACATAAAG 
AACATCAACT TCCACCGTCA GACCGAGGAG AGGTCCCTGG CCCAGCTGAA AAGACAGATG 
AAGCGCATCC GTGCCAAACA GGAGAAATAC CGGCAGAGCC AGGCAAGCCG TGGTCAACTC 
CAGTCCACAG ACCCTCAGGA GCCAGGGCCT GACAGCCCAG GGGGTTCCTC CCCGCCACGG 
ACACAGTGGT GGCGCCCCTG GCAGGACCAC GCCACAGTCA TCCACTCTGG TGACTACTTC 
CTGTTTGAGT CGGACAGTGA GGAGGAAGAG GAGGCCCTGC CTGAGGACCC CAGGCCTGCA 
GCTCAGAGTG CCTTCCAGAT GGCATACCAG GCATGGGTAA CCAACGCCCA GACAGTGCTA 
AGGCAGCGTC GTGAACAGGC ACGGCGGGAT CGGGCAGAAC AGCTGGCTTC TGGAGGCGAC 
TTGAGTCCTG AGGTGGAGCT GGTAGACGTC CCAGAGAATG AGATGGCAGG CCATAGCCAT 
GTGATGCAGC GCGTGCTGAG TACCATGCAG TTCCTGTGGG TGCTGGGCCA GGCCACAGTG 
GACGGGCTGA CGCGCTGGTT GCGTACGTTC ACGAAGGACC ACCGCACCAT GAGTGATGTG 
CTGTGTGCAG AGCGTTACCT GCTCACCCAG GAGCTTCTTC GGGGTGGAGA GGTACACCGA 
GGGGTACTGG ACCAGCTTTA CGTGAGCGAA GATGAGATCG CACTGTCAGG TCCTGTGGAG 
AACCGTGATG GACCCAGCAC AGCCTCTAGC GGGCTGGGAG CTGAAGAGCC TTTGAGTAGC 
ATGACAGACG ACACCGGCAG CCCCCTTAGC ACTGGCTACA ACACTCGCAG TGGTAGTGAG 
GAGATTATCA CCGACACTGG GGGCCTCCAG GCTGGGACCT CCCTGCACGG CTCCCAAGAG 
CTTCTAGCCA ATGCTCGTAC CCGGATGCGC ACAGCCAGTG AGCTGCTGCT GGACAGGCGC 
CTGCGTATCC CTGAGCTGGA GGAGGCCGAG CAGTTTGAGG CACAGCAGGG CCGGACGCTG 
CGGCTGCTCA GAGCCATGTA CCAGTGTGTG GCGGCCCACT CAGAGCTTCT CTGTTACTTC 
ATCATCATCC TTAACCACAT GGTGACAGCC TCGGCTGCCT CCCTGGTGCT GCCTGTGCTT 
GTGTTCCTCT GGGCCATGCT GACCATCCCG AGGCCTAGCA AGCGCTTCTG GATGACAGCT 
ATCGTCTTCA CTGAGGTCAT GGTGGTCACC AAATACTTGT TCCAGTTTGG CTTCTTCCCC 
TGGAACAGCT ACATAGTGCT TCGACGCTAC GAGAACAAGC CCTACTTCCC TCCGCGCATC 
CTGGGCCTCG AGAAAACAGA CAGCTATATC AAGTACGACC TGGTGCAACT CATGGCCCTC 
TTCTTCCACC GCTCGCAGTT ACTGTGCTAT GGCCTCTGGG ACCATGAGGA GGATGGTGTC 
CCCAAGGACC ATTGCAGGAG TAGCGAAAAG GACCAGGAAG CTGAGGAAGA GTCAGAGGCT 
AAGCTGGAAT CGCAGCCTGA GACGGGCACT GGGCATCCTG AGGAGCCCGT GTTGACTGGT 
ACCCCCAAGG ACCACATCCA AGGGAAAGGA AGTGTTAGGT CCAAGGATGA GATCCAAGAT 
CCCCCAGAGG ACCTTAAGCC CCAGCACAGG AGACACATCA GCATACGCTT CAGGAGGAGG 
AAGGAGACTC AAGGACCCAA AGGAGCAGCA GTCGTGGAGG CTGAGCACGA GGAGGGAGAG 
GAAGGAAGAG AAGCTGCAGG GAGAAAGAGG CTGCGTCGCC CTCGAGAAGG GTTGAAGATT 
CGGGAGAAAA TGAAGGCAGC TGGACGCCGG CTGCAGAGCT TCTGTCTGTC ACTGGCCCAG 
AGCTTTTACC AACCCCTGCG GCGTTTCTTC CATGACATTC TGCACACCAA GTACCGAGCG 
GCCACCGACG TCTATGCCCT CATGTTCCTG GCTGACATTG TCGACATTGT CGTCATCATC 
TTCGGTTTTT GGGCTTTTGG GAAGCACTCC GCGGCCACGG ACATTGCATC CTCACTGTCA 
GATGACCAGG TGCCACAGGC CTTCCTGTTC ATGCTGCTGG TCCAGTTCGG CACCATGGTC 
ATAGACCGCG CCCTCTACCT GCGTAAGACT GTACTGGGCA ACCTGGCCTT TCAGGTGGTC 
CTGGTGGTGG CCATTCACCT CTGGATGTTT TTCATCTTAC CGGCTGTCAC CGAGAGGATG 
TTCAGACAGA ACGCGGTGGC CCAGCTGTGG TACTTCGTGA AGTGCATTTA CTTTGCCCTG 
TCCGCCTACC AGATCCGCTG TGGCTACCCC ACCCGTATCT TGGGCAACTT CCTCACCAAG 
AAGTACAATC ATCTAAACCT CTTCCTTTTC CAGGGGTTCC GTCTAGTGCC ATTCCTGGTG 
GAGCTGCGGG CCGTCATGGA CTGGGTATGG ACTGACACCA CGCTGTCCCT GTCCAACTGG 
ATGTGTGTGG AAGACATCTA TGCCAACATC TTTATCATCA AGTGCAGCCG AGAGACAGAG 
AAGAAATACC CCCAGCCCAA GGGACAGAAG AAGAAGAAAA TCGTCAAGTA TGGCATGGGA 
GGTCTCATCA TCCTCTTCCT CATTGCCATC ATCTGGTTCC CTCTGCTCTT CATGTCACTG 
ATCCGCTCTG TGGTTGGTGT CGTCAACCAG CCCATTGATG TCACTGTTAC CCTCAAGTTA 
GGTGGCTACG AGCCACTGTT CACCATGAGC GCCCAGCAGC CATCCATAGT GCCATTCACT 
CCCGAGGACT ATGAGGAGCT GTCCCAGCAG TTTGACCCCT ACCCACTAGC CATGCAGTTC 
ATTAGCCAGT ACAGTCCTGA GGACATCGTC ACTGCACAGA TTGAGGGCAG CTCGGGGGCC 
CTGTGGCGCA TCAGCCCGCC CAGCCGGGCC CAGATGAAGC ACGAGCTGTA CAACGGCACA 
GCCGACATCA CACTGCGCTT CACCTGGAAT TTCCAAAGGG ACCTGGCCAA GGGCGGCTCT 
GTGGAGTATA CCAACGAGAA GCACACCTTG GAGCTGGCCC CCAACAGTAC GGCACGAAGG 
CAGCTGGCCC AACTGCTCGA GGGCAGACCT GACCAGTCAG TGGTCATTCC CCACCTCTTC 
CCCAAGTACA TTCGTGCACC CAATGGGCCT GAAGCCAATC CTGTGAAGCA ACTGCAGCCA 
GACGAGGAAG AGGACTACCT TGGTGTGCGT ATCCAGCTGC GGAGAGAGCA AGTGGGCACA 
GGAACCTCTG GGGAGCAAGC AGGCACAAAG GCCTCCGACT TCCTCGAGTG GTGGGTGATT 
GAGCTGCAGG ACTGCCAGGC CGAGTGCAAC CTGCTGCCCA TGGTCATCTT CAGTGACAAG 
GTCAGCCCGC CTAGCCTGGG CTTCCTGGCC GGCTACGGGA TTGTGGGGCT CTACGTCTCT 
ATCGTGCTGG TCGTTGGCAA GTTTGTGCGG GGCTTCTTCA GTGATATCTC ACACTCCATC 
ATGTTCGAGG AGCTGCCGTG CGTGGACCGC ATCCTCAAGC TGTGCCAGGA CATCTTCTTG 
GTACGGGAGA CCCGCGAGCT GGAGCTGGAG GAGGAACTCT ATGCCAAGCT CATATTCCTG 
TACCGATCAC CAGAGACCAT GATCAAGTGG ACGCGTGAGA AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piezo1 ( XM_008772626.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770848.1 Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X1, mRNA.

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CloneID	ORa47912
Clone ID Related Accession (Same CDS sequence)	XM_008772626.2
Accession Version	XM_008772626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	piezo-type mechanosensitive ion channel component 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG 
CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT 
CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC 
CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGCAGCAG CCTTTGGGCA 
AACGTGTCTC AACACATAGG GGTTACAAGG CTGGACCTGA AGGACATCTT TAACACCACC 
AGACTGGTGG CACCTGACCT GGGAGTGCTG GTGGCATCTT CCCTTTGCCT TGGCCTCTGT 
GGACGCCTCA CAAGGAAAGC CCGGCAGAGC CAGCGCACTC AGGAGCTGCA GGAGGAGGAT 
GACATAGATG CTGCCCCCGC CGCAGGGCTG CAGGGAGCCC CCACCCTGGC AACCAAACGC 
AGGCTGTGGC TGGCCCCTCG CTTCCGGATC ACGGCGCACT GGCTGCTGGT GACCTCCGGA 
CGGATGCTGG TCATCGTGCT ACTGGCACTC GCAGGCATAG CCCATCCTTC GGCCTTCTCC 
AGCGTCTACC TGATGGTGTT CCTGGCCATC TGCACCTGGT GGTCTTGCCA CTTTCCTCTC 
AGCTCCCTGG GCTTTAACAC CCTCTGCGTC ATGGTGAGCT GCTTTGGCGC CGGCCATCTT 
GTTTGTTTAT ACTGCTATCA GACACCATTT GTCCAGAGCG TGCTACTTCC TGGCAGCCTC 
TGGGCCAGGC TATTTGGTCT CAAGAACTTC GTAGACATCC CTAACTGCTC CAGCCCCAAC 
GTGCTGGTAC TCAACACTAA GCATGCTTGG CCCATCTACG TGAGCCCCGG TATCCTGCTG 
CTGTTGTACT ACACAGCCAC CTCTCTCCTG AAGCTCCGCA AGGGCCGCTT CTCAGAGCTG 
AGGAAGGAGA TCCCGAGGGA GGATGAGGAG CATGAACTGG AACTGGACCA GCTGGAGCCA 
GAGCCCCAGG CTAGGGGCAC CACCCAGGGT GCGACGCCCA CGACCACTGG ACCCGACATT 
GACAACTGCA CCGTGCATGT ACTAACCAGC CAGAGCCCCG TCCGCCAGCG TCCTGTACGC 
CCCAGGCTGG CTGAGCTGAA AGAGATGTCA CCACTACATG GCCTGGGCCA CCTCATCCTG 
GACCAGAGCT ATGTGTGTGC TCTGATCGCC ATGATGGTGT GGAGCATCAT GTACCACAGC 
TGGCTGACCT TCGTCTTGCT GCTCTGGGCC TGCCTCATCT GGACCGTGCG CAGCCGCCAC 
CAGTTGGCTA TGCTGTGCTC ACCCTGCATC CTGCTGTACG GACTGACACT CTGCTGCCTG 
CGGTACGTGT GGGCCATGGA GCTCCCCGAG CTGCCCACCA CCCTGGGCCC TGTCAGTCTA 
CATCAGTTGG GATTGGAGCA TACACGCTAC CCCTGCCTGG ACCTTGGTGC CATGCTGCTG 
TATCTGCTCA CGTTCTGGCT GCTCCTGCGT CAGTTCGTGA AGGAGAAGCT GCTGAAGAAG 
AGAAAGGCAC CTTCGACGCT GCTGGAGGTC ACAGTGTCCG ACACAGAGCC CACACAGACC 
CAGACACTGC TGAGGAGCCT GGGGGAGCTG GTCACGGGCA TCTACGTCAA GTACTGGATC 
TACGTGTGCG CTGGGATGTT CATTGTGGTC AGCTTCGCTG GCCGCCTGGT GGTCTACAAA 
ATCGTCTACA TGTTCCTTTT CCTGCTGTGC CTCACGTTGT TCCAGGTCTA CTACACTCTG 
TGGAGGAAGC TGCTCCGAGT TTTCTGGTGG CTCGTGGTGG CCTACACCAT GCTCGTGCTC 
ATCGCCGTGT ACACTTTCCA GTTCCAGGAC TTCCCCACCT ACTGGCGCAA CCTCACGGGC 
TTCACAGATG AACAGTTGGG CGACCTGGGC CTGGAGCAGT TCAGCGTGTC GGAGCTCTTT 
TCCAGCATCC TCATCCCTGG CTTCTTCCTG CTGGCCTGCA TCCTGCAGCT GCACTACTTC 
CACAGGCCGT TCATGCAGCT CACCGACCTG GAGCACGTGC CCCCGCCAGG CACACGCCGC 
CTTCGCTGGG CTCACAGGCA GGATACAGTG AGCGAGGCCC CTCTGCTTCA GCATCAGGAG 
GAGGAGGAAG TCTTCAGGGA TGACGGGCAG AGCATGGATG GGCCCCACCA GACCACACAG 
GTCCCTGAGG GTACAGCCAG CAAGTGGGGC CTGGTGGCCG ACCGGCTGCT GGACCTAGCG 
TCCAGCTTCT CCGCCGTCCT CACCCGCATC CAGGTGTTCG TACGATGTTT GCTCGAGCTT 
CACGTCTTCA AGCTGGTGGC TCTCTACACT GTCTGGGTGG CCCTGAAAGA AGTGTCCGTG 
ATGAACCTGC TGCTGGTGGT GCTGTGGGCC TTCGCCCTGC CCTACCCGCG CTTCCGGCCC 
ATGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT 
CAGCTGAAGA TTGTCAACCC ACACGAGTAC TCCAGTAACT GCACCGAGCC CTTTCCCAAC 
AATACCAACT TGCAGCCCTT GGAGATCAGC CAGTCTCTGC TGTACCGTGG CCCCGTTGAC 
CCTGCCAACT GGTTTGGGGT GCGGAAGGGT TACCCAAACT TGGGCTATAT CCAGAACCAC 
CTGCAGATCC TTCTGCTGCT GGTGTTTGAG GCTGTGGTGT ACCGGCGCCA AGAGCACTAC 
CGCCGGCAGC ACCAGCAGGC CCCTCTGCCC GCCCAGGCTT TGTGCGCTGA TGGCACCCGC 
CAGAGGCTGG ACCAGGACCT ACTCAGCTGC CTCAAATATT TCATCAACTT CTTCTTCTAC 
AAATTCGGGC TGGAGATCTG CTTCTTGATG GCCGTGAATG TCATCGGACA ACGCATGAAC 
TTCATGGTGA TCTTACACGG CTGCTGGTTG GTGGCCATCC TTACACGCCG GCGCCGTGAG 
GCCATCGCCC GCCTCTGGCC CAATTACTGT CTGTTCCTCA CGCTGTTCCT GCTGTACCAG 
TACCTGCTGT GCCTGGGCAT GCCTCCCGCA CTGTGCATCG ACTACCCGTG GCGCTGGAGC 
CAGGCCATCC CCATGAATTC TGCGCTCATC AAGTGGCTGT ACCTCCCTGA CTTCTTCAGG 
GCCCCCAACT CCACCAACCT TATCAGTGAC TTCCTCCTGC TACTCTGCGC CTCCCAACAG 
TGGCAGGTGT TCTCCGCAGA GCAAACGGAA GAATGGCAGC GCATGGCTGG CGTTAACACT 
GACCACCTGG AGCCCCTGCG TGGGGAGCCC AACCCTATCC CCAACTTCAT CCACTGCAGG 
TCCTATCTGG ATATGCTGAA GGTGGCCGTC TTCCGCTACC TGTTCTGGCT GGTGCTGGTG 
GTCGTGTTTG TCACAGGGGC CACCCGCATC AGCATCTTTG GGCTGGGGTA CCTGCTGGCC 
TGCTTCTACC TGCTGCTCTT TGGCACCACC CTGCTGCAGA AGGACACACG AGCCCAGCTC 
GTGCTGTGGG ACTGCCTCAT CCTCTACAAC GTCACCGTCA TCATCTCTAA GAATATGTTG 
TCGCTCCTGT CCTGTGTCTT CGTGGAGCAA ATGCAGAGCA ACTTCTGCTG GGTCATCCAG 
CTCTTCAGCC TCGTGTGCAC AGTCAAAGGC TACTATGACC CCAAAGAGAT GAAGACCAGG 
GACCGGGATT GCCTGCTGCC TGTGGAGGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC 
TTCTTCCTGC TCCTACAACG GCGCGTCTTT CTCAGCCACT ACTTCCTGCA CGTCAGTGCT 
GACCTGAAGG CCACGGCCCT GCAGGCCTCC AGGGGTTTTG CTCTCTACAA TGCGGCCAAC 
ATAAAGAACA TCAACTTCCA CCGTCAGACC GAGGAGAGGT CCCTGGCCCA GCTGAAAAGA 
CAGATGAAGC GCATCCGTGC CAAACAGGAG AAATACCGGC AGAGCCAGGC AAGCCGTGGT 
CAACTCCAGT CCACAGACCC TCAGGAGCCA GGGCCTGACA GCCCAGGGGG TTCCTCCCCG 
CCACGGACAC AGTGGTGGCG CCCCTGGCAG GACCACGCCA CAGTCATCCA CTCTGGTGAC 
TACTTCCTGT TTGAGTCGGA CAGTGAGGAG GAAGAGGAGG CCCTGCCTGA GGACCCCAGG 
CCTGCAGCTC AGAGTGCCTT CCAGATGGCA TACCAGGCAT GGGTAACCAA CGCCCAGACA 
GTGCTAAGGC AGCGTCGTGA ACAGGCACGG CGGGATCGGG CAGAACAGCT GGCTTCTGGA 
GGCGACTTGA GTCCTGAGGT GGAGCCGGTA GACGTCCCAG AGAATGAGAT GGCAGGCCGT 
AGCCATGTGA TGCAGCGCGT GCTGAGTACC ATGCAGTTCC TGTGGGTGCT GGGCCAGGCC 
ACAGTGGACG GGCTGACGCG CTGGTTGCGT ACGTTCACGA AGGACCACCG CACCATGAGT 
GATGTGCTGT GTGCAGAGCG TTACCTGCTC ACCCAGGAGC TTCTTCGGGG TGGAGAGGTA 
CACCGAGGGG TACTGGACCA GCTTTACGTG AGCGAAGATG AGATCGCACT GTCAGGTCCT 
GTGGAGAACC GTGATGGACC CAGCACAGCC TCTAGCGGGC TGGGAGCTGA AGAGCCTTTG 
AGTAGCATGA CAGACGACAC CGGCAGCCCC CTTAGCACTG GCTACAACAC TCGCAGTGGT 
AGTGAGGAGA TTATCACCGA CACTGGGGGC CTCCAGGCTG GGACCTCCCT GCACGGCTCC 
CAAGAGCTTC TAGCCAATGC TCGTACCCGG ATGCGCACAG CCAGTGAGCT GCTGCTGGAC 
AGGCGCCTGC GTATCCCTGA GCTGGAGGAG GCCGAGCAGT TTGAGGCACA GCAGGGCCGG 
ACGCTGCGGC TGCTCAGAGC CATGTACCAG TGTGTGGCGG CCCACTCAGA GCTTCTCTGT 
TACTTCATCA TCATCCTTAA CCACATGGTG ACAGCCTCGG CTGCCTCCCT GGTGCTGCCT 
GTGCTTGTGT TCCTCTGGGC CATGCTGACC ATCCCGAGGC CTAGCAAGCG CTTCTGGATG 
ACAGCTATCG TCTTCACTGA GGTCATGGTG GTCACCAAAT ACTTGTTCCA GTTTGGCTTC 
TTCCCCTGGA ACAGCTACAT AGTGCTTCGA CGCTACGAGA ACAAGCCCTA CTTCCCTCCG 
CGCATCCTGG GCCTCGAGAA AACGGACAGC TATATCAAGT ACGACCTGGT GCAACTCATG 
GCCCTCTTCT TCCACCGCTC GCAGTTACTG TGCTATGGCC TCTGGGACCA TGAGGAGGAT 
GGTGTCCCCA AGGACCATTG CAGGAGTAGC GAAAAGGACC AAGAAGCTGA GGAAGAGTCA 
GAGGCTAAGC TGGAATCGCA GCCTGAGACG GGCACTGGGC ATCCTGAGGA GCCCGTGTTG 
ACTGGTACCC CCAAGGACCA CATCCAAGGG AAAGGAAGTG TTAGGTCCAA GGATGAGATC 
CAAGATCCCC CAGAGGACCT TAAGCCCCAG CACAGGAGAC ACATCAGCAT ACGCTTCAGG 
AGGAGGAAGG AGACTCAAGG ACCCAAAGGA GCAGCAGTCG TGGAGGCTGA GCACGAGGAG 
GGAGAGGAAG GAAGAGAAGC TGCAGGGAGA AAGAGGCTGC GTCGCCCTCG AGAAGGGTTG 
AAGATTCGGG AGAAAATGAA GGCAGCTGGA CGCCGGCTGC AGAGCTTCTG TCTGTCACTG 
GCCCAGAGCT TTTACCAACC CCTGCGGCGT TTCTTCCATG ACATTCTGCA CACCAAGTAC 
CGAGCGGCCA CCGACGTCTA TGCCCTCATG TTCCTGGCTG ACATTGTCGA CATTGTCGTC 
ATCATCTTCG GTTTTTGGGC TTTTGGGAAG CACTCCGCGG CCACGGACAT TGCATCCTCA 
CTGTCAGATG ACCAGGTGCC ACAGGCCTTC CTGTTCATGC TGCTGGTCCA GTTCGGCACC 
ATGGTCATAG ACCGCGCCCT CTACCTGCGT AAGACTGTAC TGGGCAAGCT GGCCTTTCAG 
GTGGTCCTGG TGGTGGCCAT TCACCTCTGG ATGTTTTTCA TCTTACCGGC TGTCACCGAG 
AGGATGTTCA GACAGAACGC GGTGGCCCAG CTGTGGTACT TCGTGAAGTG CATTTACTTT 
GCCCTGTCCG CCTACCAGAT CCGCTGTGGC TACCCCACCC GTATCTTGGG CAACTTCCTC 
ACCAAGAAGT ACAATCATCT AAACCTCTTC CTTTTCCAGG GGTTCCGTCT AGTGCCATTC 
CTGGTGGAGC TGCGGGCCGT CATGGACTGG GTATGGACTG ACACCACGCT GTCCCTGTCC 
AACTGGATGT GTGTGGAAGA CATCTATGCC AACATCTTTA TCATCAAGTG CAGCCGAGAG 
ACAGAGAAGA AATACCCCCA GCCCAAGGGA CAGAAGAAGA AGAAAATCGT CAAGTATGGC 
ATGGGAGGTC TCATCATCCT CTTCCTCATT GCCATCATCT GGTTCCCTCT GCTCTTCATG 
TCACTGATCC GCTCTGTGGT TGGTGTCGTC AACCAGCCCA TTGATGTCAC TGTTACCCTC 
AAGTTAGGTG GCTACGAGCC ACTGTTCACC ATGAGCGCCC AGCAGCCATC CATAGTGCCA 
TTCACTCCCG AGGACTATGA GGAGCTGTCC CAGCAGTTTG ACCCCTACCC ACTAGCCATG 
CAGTTCATTA GCCAGTACAG TCCTGAGGAC ATCGTCACTG CACAGATTGA GGGCAGCTCG 
GGGGCCCTGT GGCGCATCAG CCCGCCCAGC CGGGCCCAGA TGAAGCACGA GCTGTACAAC 
GGCACAGCCG ACATCACACT GCGCTTCACC TGGAATTTCC AAAGGGACCT GGCCAAGGGC 
GGCTCTGTGG AGTATACCAA CGAGAAGCAC ACCTTGGAGC TGGCCCCCAA CAGTACGGCA 
CGAAGGCAGC TGGCCCAACT GCTCGAGGGC AGACCTGACC AGTCAGTGGT CATTCCCCAC 
CTCTTCCCCA AGTACATTCG TGCACCCAAT GGGCCTGAAG CCAATCCTGT GAAGCAACTG 
CAGCCAGACG AGGAAGAGGA CTACCTTGGT GTGCGTATCC AGCTGCGGAG AGAGCAAGTG 
GGCACAGGAA CCTCTGGGGA GCAAGCAGGC ACAAAGGCCT CCGACTTCCT CGAGTGGTGG 
GTGATTGAGC TGCAGGACTG CCAGGCCGAG TGCAACCTGC TGCCCATGGT CATCTTCAGT 
GACAAGGTCA GCCCGCCTAG CCTGGGCTTC CTGGCCGGCT ACGGGATTGT GGGGCTCTAC 
GTCTCTATCG TGCTGGTCGT TGGCAAGTTT GTGCGGGGCT TCTTCAGTGA TATCTCACAC 
TCCATCATGT TCGAGGAGCT GCCGTGCGTG GACCGCATCC TCAAGCTGTG CCAGGACATC 
TTCTTGGTAC GGGAGACCCG AGAGCTGGAG CTGGAGGAGG AACTCTATGC CAAGCTCATA 
TTCCTGTACC GATCACCAGA GACCATGATC AAGTGGACGC GTGAGAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770848.1
CDS	618..8231
Translation	MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWLPGPSRHSIPGHTGRLLRALLCLSLLFLVAHVAFQICLHTMPRLNQLLGQNCSSLWANVSQHIGVTRLDLKDIFNTTRLVAPDLGVLVASSLCLGLCGRLTRKARQSQRTQELQEEDDIDAAPAAGLQGAPTLATKRRLWLAPRFRITAHWLLVTSGRMLVIVLLALAGIAHPSAFSSVYLMVFLAICTWWSCHFPLSSLGFNTLCVMVSCFGAGHLVCLYCYQTPFVQSVLLPGSLWARLFGLKNFVDIPNCSSPNVLVLNTKHAWPIYVSPGILLLLYYTATSLLKLRKGRFSELRKEIPREDEEHELELDQLEPEPQARGTTQGATPTTTGPDIDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLHGLGHLILDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFVKEKLLKKRKAPSTLLEVTVSDTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPTYWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHVPPPGTRRLRWAHRQDTVSEAPLLQHQEEEEVFRDDGQSMDGPHQTTQVPEGTASKWGLVADRLLDLASSFSAVLTRIQVFVRCLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEISQSLLYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQALCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSQAIPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAEQTEEWQRMAGVNTDHLEPLRGEPNPIPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTTLLQKDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYDPKEMKTRDRDCLLPVEEAGIIWDSICFFFLLLQRRVFLSHYFLHVSADLKATALQASRGFALYNAANIKNINFHRQTEERSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSTDPQEPGPDSPGGSSPPRTQWWRPWQDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQMAYQAWVTNAQTVLRQRREQARRDRAEQLASGGDLSPEVEPVDVPENEMAGRSHVMQRVLSTMQFLWVLGQATVDGLTRWLRTFTKDHRTMSDVLCAERYLLTQELLRGGEVHRGVLDQLYVSEDEIALSGPVENRDGPSTASSGLGAEEPLSSMTDDTGSPLSTGYNTRSGSEEIITDTGGLQAGTSLHGSQELLANARTRMRTASELLLDRRLRIPELEEAEQFEAQQGRTLRLLRAMYQCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVMVVTKYLFQFGFFPWNSYIVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQLLCYGLWDHEEDGVPKDHCRSSEKDQEAEEESEAKLESQPETGTGHPEEPVLTGTPKDHIQGKGSVRSKDEIQDPPEDLKPQHRRHISIRFRRRKETQGPKGAAVVEAEHEEGEEGREAAGRKRLRRPREGLKIREKMKAAGRRLQSFCLSLAQSFYQPLRRFFHDILHTKYRAATDVYALMFLADIVDIVVIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLRKTVLGKLAFQVVLVVAIHLWMFFILPAVTERMFRQNAVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPEDYEELSQQFDPYPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKHELYNGTADITLRFTWNFQRDLAKGGSVEYTNEKHTLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLGVRIQLRREQVGTGTSGEQAGTKASDFLEWWVIELQDCQAECNLLPMVIFSDKVSPPSLGFLAGYGIVGLYVSIVLVVGKFVRGFFSDISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_008772626.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772626.2

ORF Insert Sequence:

ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG 
CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT 
CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC 
CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGCAGCAG CCTTTGGGCA 
AACGTGTCTC AACACATAGG GGTTACAAGG CTGGACCTGA AGGACATCTT TAACACCACC 
AGACTGGTGG CACCTGACCT GGGAGTGCTG GTGGCATCTT CCCTTTGCCT TGGCCTCTGT 
GGACGCCTCA CAAGGAAAGC CCGGCAGAGC CAGCGCACTC AGGAGCTGCA GGAGGAGGAT 
GACATAGATG CTGCCCCCGC CGCAGGGCTG CAGGGAGCCC CCACCCTGGC AACCAAACGC 
AGGCTGTGGC TGGCCCCTCG CTTCCGGATC ACGGCGCACT GGCTGCTGGT GACCTCCGGA 
CGGATGCTGG TCATCGTGCT ACTGGCACTC GCAGGCATAG CCCATCCTTC GGCCTTCTCC 
AGCGTCTACC TGATGGTGTT CCTGGCCATC TGCACCTGGT GGTCTTGCCA CTTTCCTCTC 
AGCTCCCTGG GCTTTAACAC CCTCTGCGTC ATGGTGAGCT GCTTTGGCGC CGGCCATCTT 
GTTTGTTTAT ACTGCTATCA GACACCATTT GTCCAGAGCG TGCTACTTCC TGGCAGCCTC 
TGGGCCAGGC TATTTGGTCT CAAGAACTTC GTAGACATCC CTAACTGCTC CAGCCCCAAC 
GTGCTGGTAC TCAACACTAA GCATGCTTGG CCCATCTACG TGAGCCCCGG TATCCTGCTG 
CTGTTGTACT ACACAGCCAC CTCTCTCCTG AAGCTCCGCA AGGGCCGCTT CTCAGAGCTG 
AGGAAGGAGA TCCCGAGGGA GGATGAGGAG CATGAACTGG AACTGGACCA GCTGGAGCCA 
GAGCCCCAGG CTAGGGGCAC CACCCAGGGT GCGACGCCCA CGACCACTGG ACCCGACATT 
GACAACTGCA CCGTGCATGT ACTAACCAGC CAGAGCCCCG TCCGCCAGCG TCCTGTACGC 
CCCAGGCTGG CTGAGCTGAA AGAGATGTCA CCACTACATG GCCTGGGCCA CCTCATCCTG 
GACCAGAGCT ATGTGTGTGC TCTGATCGCC ATGATGGTGT GGAGCATCAT GTACCACAGC 
TGGCTGACCT TCGTCTTGCT GCTCTGGGCC TGCCTCATCT GGACCGTGCG CAGCCGCCAC 
CAGTTGGCTA TGCTGTGCTC ACCCTGCATC CTGCTGTACG GACTGACACT CTGCTGCCTG 
CGGTACGTGT GGGCCATGGA GCTCCCCGAG CTGCCCACCA CCCTGGGCCC TGTCAGTCTA 
CATCAGTTGG GATTGGAGCA TACACGCTAC CCCTGCCTGG ACCTTGGTGC CATGCTGCTG 
TATCTGCTCA CGTTCTGGCT GCTCCTGCGT CAGTTCGTGA AGGAGAAGCT GCTGAAGAAG 
AGAAAGGCAC CTTCGACGCT GCTGGAGGTC ACAGTGTCCG ACACAGAGCC CACACAGACC 
CAGACACTGC TGAGGAGCCT GGGGGAGCTG GTCACGGGCA TCTACGTCAA GTACTGGATC 
TACGTGTGCG CTGGGATGTT CATTGTGGTC AGCTTCGCTG GCCGCCTGGT GGTCTACAAA 
ATCGTCTACA TGTTCCTTTT CCTGCTGTGC CTCACGTTGT TCCAGGTCTA CTACACTCTG 
TGGAGGAAGC TGCTCCGAGT TTTCTGGTGG CTCGTGGTGG CCTACACCAT GCTCGTGCTC 
ATCGCCGTGT ACACTTTCCA GTTCCAGGAC TTCCCCACCT ACTGGCGCAA CCTCACGGGC 
TTCACAGATG AACAGTTGGG CGACCTGGGC CTGGAGCAGT TCAGCGTGTC GGAGCTCTTT 
TCCAGCATCC TCATCCCTGG CTTCTTCCTG CTGGCCTGCA TCCTGCAGCT GCACTACTTC 
CACAGGCCGT TCATGCAGCT CACCGACCTG GAGCACGTGC CCCCGCCAGG CACACGCCGC 
CTTCGCTGGG CTCACAGGCA GGATACAGTG AGCGAGGCCC CTCTGCTTCA GCATCAGGAG 
GAGGAGGAAG TCTTCAGGGA TGACGGGCAG AGCATGGATG GGCCCCACCA GACCACACAG 
GTCCCTGAGG GTACAGCCAG CAAGTGGGGC CTGGTGGCCG ACCGGCTGCT GGACCTAGCG 
TCCAGCTTCT CCGCCGTCCT CACCCGCATC CAGGTGTTCG TACGATGTTT GCTCGAGCTT 
CACGTCTTCA AGCTGGTGGC TCTCTACACT GTCTGGGTGG CCCTGAAAGA AGTGTCCGTG 
ATGAACCTGC TGCTGGTGGT GCTGTGGGCC TTCGCCCTGC CCTACCCGCG CTTCCGGCCC 
ATGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT 
CAGCTGAAGA TTGTCAACCC ACACGAGTAC TCCAGTAACT GCACCGAGCC CTTTCCCAAC 
AATACCAACT TGCAGCCCTT GGAGATCAGC CAGTCTCTGC TGTACCGTGG CCCCGTTGAC 
CCTGCCAACT GGTTTGGGGT GCGGAAGGGT TACCCAAACT TGGGCTATAT CCAGAACCAC 
CTGCAGATCC TTCTGCTGCT GGTGTTTGAG GCTGTGGTGT ACCGGCGCCA AGAGCACTAC 
CGCCGGCAGC ACCAGCAGGC CCCTCTGCCC GCCCAGGCTT TGTGCGCTGA TGGCACCCGC 
CAGAGGCTGG ACCAGGACCT ACTCAGCTGC CTCAAATATT TCATCAACTT CTTCTTCTAC 
AAATTCGGGC TGGAGATCTG CTTCTTGATG GCCGTGAATG TCATCGGACA ACGCATGAAC 
TTCATGGTGA TCTTACACGG CTGCTGGTTG GTGGCCATCC TTACACGCCG GCGCCGTGAG 
GCCATCGCCC GCCTCTGGCC CAATTACTGT CTGTTCCTCA CGCTGTTCCT GCTGTACCAG 
TACCTGCTGT GCCTGGGCAT GCCTCCCGCA CTGTGCATCG ACTACCCGTG GCGCTGGAGC 
CAGGCCATCC CCATGAATTC TGCGCTCATC AAGTGGCTGT ACCTCCCTGA CTTCTTCAGG 
GCCCCCAACT CCACCAACCT TATCAGTGAC TTCCTCCTGC TACTCTGCGC CTCCCAACAG 
TGGCAGGTGT TCTCCGCAGA GCAAACGGAA GAATGGCAGC GCATGGCTGG CGTTAACACT 
GACCACCTGG AGCCCCTGCG TGGGGAGCCC AACCCTATCC CCAACTTCAT CCACTGCAGG 
TCCTATCTGG ATATGCTGAA GGTGGCCGTC TTCCGCTACC TGTTCTGGCT GGTGCTGGTG 
GTCGTGTTTG TCACAGGGGC CACCCGCATC AGCATCTTTG GGCTGGGGTA CCTGCTGGCC 
TGCTTCTACC TGCTGCTCTT TGGCACCACC CTGCTGCAGA AGGACACACG AGCCCAGCTC 
GTGCTGTGGG ACTGCCTCAT CCTCTACAAC GTCACCGTCA TCATCTCTAA GAATATGTTG 
TCGCTCCTGT CCTGTGTCTT CGTGGAGCAA ATGCAGAGCA ACTTCTGCTG GGTCATCCAG 
CTCTTCAGCC TCGTGTGCAC AGTCAAAGGC TACTATGACC CCAAAGAGAT GAAGACCAGG 
GACCGGGATT GCCTGCTGCC TGTGGAGGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC 
TTCTTCCTGC TCCTACAACG GCGCGTCTTT CTCAGCCACT ACTTCCTGCA CGTCAGTGCT 
GACCTGAAGG CCACGGCCCT GCAGGCCTCC AGGGGTTTTG CTCTCTACAA TGCGGCCAAC 
ATAAAGAACA TCAACTTCCA CCGTCAGACC GAGGAGAGGT CCCTGGCCCA GCTGAAAAGA 
CAGATGAAGC GCATCCGTGC CAAACAGGAG AAATACCGGC AGAGCCAGGC AAGCCGTGGT 
CAACTCCAGT CCACAGACCC TCAGGAGCCA GGGCCTGACA GCCCAGGGGG TTCCTCCCCG 
CCACGGACAC AGTGGTGGCG CCCCTGGCAG GACCACGCCA CAGTCATCCA CTCTGGTGAC 
TACTTCCTGT TTGAGTCGGA CAGTGAGGAG GAAGAGGAGG CCCTGCCTGA GGACCCCAGG 
CCTGCAGCTC AGAGTGCCTT CCAGATGGCA TACCAGGCAT GGGTAACCAA CGCCCAGACA 
GTGCTAAGGC AGCGTCGTGA ACAGGCACGG CGGGATCGGG CAGAACAGCT GGCTTCTGGA 
GGCGACTTGA GTCCTGAGGT GGAGCCGGTA GACGTCCCAG AGAATGAGAT GGCAGGCCGT 
AGCCATGTGA TGCAGCGCGT GCTGAGTACC ATGCAGTTCC TGTGGGTGCT GGGCCAGGCC 
ACAGTGGACG GGCTGACGCG CTGGTTGCGT ACGTTCACGA AGGACCACCG CACCATGAGT 
GATGTGCTGT GTGCAGAGCG TTACCTGCTC ACCCAGGAGC TTCTTCGGGG TGGAGAGGTA 
CACCGAGGGG TACTGGACCA GCTTTACGTG AGCGAAGATG AGATCGCACT GTCAGGTCCT 
GTGGAGAACC GTGATGGACC CAGCACAGCC TCTAGCGGGC TGGGAGCTGA AGAGCCTTTG 
AGTAGCATGA CAGACGACAC CGGCAGCCCC CTTAGCACTG GCTACAACAC TCGCAGTGGT 
AGTGAGGAGA TTATCACCGA CACTGGGGGC CTCCAGGCTG GGACCTCCCT GCACGGCTCC 
CAAGAGCTTC TAGCCAATGC TCGTACCCGG ATGCGCACAG CCAGTGAGCT GCTGCTGGAC 
AGGCGCCTGC GTATCCCTGA GCTGGAGGAG GCCGAGCAGT TTGAGGCACA GCAGGGCCGG 
ACGCTGCGGC TGCTCAGAGC CATGTACCAG TGTGTGGCGG CCCACTCAGA GCTTCTCTGT 
TACTTCATCA TCATCCTTAA CCACATGGTG ACAGCCTCGG CTGCCTCCCT GGTGCTGCCT 
GTGCTTGTGT TCCTCTGGGC CATGCTGACC ATCCCGAGGC CTAGCAAGCG CTTCTGGATG 
ACAGCTATCG TCTTCACTGA GGTCATGGTG GTCACCAAAT ACTTGTTCCA GTTTGGCTTC 
TTCCCCTGGA ACAGCTACAT AGTGCTTCGA CGCTACGAGA ACAAGCCCTA CTTCCCTCCG 
CGCATCCTGG GCCTCGAGAA AACGGACAGC TATATCAAGT ACGACCTGGT GCAACTCATG 
GCCCTCTTCT TCCACCGCTC GCAGTTACTG TGCTATGGCC TCTGGGACCA TGAGGAGGAT 
GGTGTCCCCA AGGACCATTG CAGGAGTAGC GAAAAGGACC AAGAAGCTGA GGAAGAGTCA 
GAGGCTAAGC TGGAATCGCA GCCTGAGACG GGCACTGGGC ATCCTGAGGA GCCCGTGTTG 
ACTGGTACCC CCAAGGACCA CATCCAAGGG AAAGGAAGTG TTAGGTCCAA GGATGAGATC 
CAAGATCCCC CAGAGGACCT TAAGCCCCAG CACAGGAGAC ACATCAGCAT ACGCTTCAGG 
AGGAGGAAGG AGACTCAAGG ACCCAAAGGA GCAGCAGTCG TGGAGGCTGA GCACGAGGAG 
GGAGAGGAAG GAAGAGAAGC TGCAGGGAGA AAGAGGCTGC GTCGCCCTCG AGAAGGGTTG 
AAGATTCGGG AGAAAATGAA GGCAGCTGGA CGCCGGCTGC AGAGCTTCTG TCTGTCACTG 
GCCCAGAGCT TTTACCAACC CCTGCGGCGT TTCTTCCATG ACATTCTGCA CACCAAGTAC 
CGAGCGGCCA CCGACGTCTA TGCCCTCATG TTCCTGGCTG ACATTGTCGA CATTGTCGTC 
ATCATCTTCG GTTTTTGGGC TTTTGGGAAG CACTCCGCGG CCACGGACAT TGCATCCTCA 
CTGTCAGATG ACCAGGTGCC ACAGGCCTTC CTGTTCATGC TGCTGGTCCA GTTCGGCACC 
ATGGTCATAG ACCGCGCCCT CTACCTGCGT AAGACTGTAC TGGGCAAGCT GGCCTTTCAG 
GTGGTCCTGG TGGTGGCCAT TCACCTCTGG ATGTTTTTCA TCTTACCGGC TGTCACCGAG 
AGGATGTTCA GACAGAACGC GGTGGCCCAG CTGTGGTACT TCGTGAAGTG CATTTACTTT 
GCCCTGTCCG CCTACCAGAT CCGCTGTGGC TACCCCACCC GTATCTTGGG CAACTTCCTC 
ACCAAGAAGT ACAATCATCT AAACCTCTTC CTTTTCCAGG GGTTCCGTCT AGTGCCATTC 
CTGGTGGAGC TGCGGGCCGT CATGGACTGG GTATGGACTG ACACCACGCT GTCCCTGTCC 
AACTGGATGT GTGTGGAAGA CATCTATGCC AACATCTTTA TCATCAAGTG CAGCCGAGAG 
ACAGAGAAGA AATACCCCCA GCCCAAGGGA CAGAAGAAGA AGAAAATCGT CAAGTATGGC 
ATGGGAGGTC TCATCATCCT CTTCCTCATT GCCATCATCT GGTTCCCTCT GCTCTTCATG 
TCACTGATCC GCTCTGTGGT TGGTGTCGTC AACCAGCCCA TTGATGTCAC TGTTACCCTC 
AAGTTAGGTG GCTACGAGCC ACTGTTCACC ATGAGCGCCC AGCAGCCATC CATAGTGCCA 
TTCACTCCCG AGGACTATGA GGAGCTGTCC CAGCAGTTTG ACCCCTACCC ACTAGCCATG 
CAGTTCATTA GCCAGTACAG TCCTGAGGAC ATCGTCACTG CACAGATTGA GGGCAGCTCG 
GGGGCCCTGT GGCGCATCAG CCCGCCCAGC CGGGCCCAGA TGAAGCACGA GCTGTACAAC 
GGCACAGCCG ACATCACACT GCGCTTCACC TGGAATTTCC AAAGGGACCT GGCCAAGGGC 
GGCTCTGTGG AGTATACCAA CGAGAAGCAC ACCTTGGAGC TGGCCCCCAA CAGTACGGCA 
CGAAGGCAGC TGGCCCAACT GCTCGAGGGC AGACCTGACC AGTCAGTGGT CATTCCCCAC 
CTCTTCCCCA AGTACATTCG TGCACCCAAT GGGCCTGAAG CCAATCCTGT GAAGCAACTG 
CAGCCAGACG AGGAAGAGGA CTACCTTGGT GTGCGTATCC AGCTGCGGAG AGAGCAAGTG 
GGCACAGGAA CCTCTGGGGA GCAAGCAGGC ACAAAGGCCT CCGACTTCCT CGAGTGGTGG 
GTGATTGAGC TGCAGGACTG CCAGGCCGAG TGCAACCTGC TGCCCATGGT CATCTTCAGT 
GACAAGGTCA GCCCGCCTAG CCTGGGCTTC CTGGCCGGCT ACGGGATTGT GGGGCTCTAC 
GTCTCTATCG TGCTGGTCGT TGGCAAGTTT GTGCGGGGCT TCTTCAGTGA TATCTCACAC 
TCCATCATGT TCGAGGAGCT GCCGTGCGTG GACCGCATCC TCAAGCTGTG CCAGGACATC 
TTCTTGGTAC GGGAGACCCG AGAGCTGGAG CTGGAGGAGG AACTCTATGC CAAGCTCATA 
TTCCTGTACC GATCACCAGA GACCATGATC AAGTGGACGC GTGAGAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piezo1 ( XM_008772627.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770849.1 Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X2, mRNA.

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CloneID	ORa47913
Clone ID Related Accession (Same CDS sequence)	XM_008772627.2
Accession Version	XM_008772627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	piezo-type mechanosensitive ion channel component 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG 
CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT 
CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC 
CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGCAGCAG CCTTTGGGCA 
AACGTGTCTC AACACATAGG GGTTACAAGG CTGGACCTGA AGGACATCTT TAACACCACC 
AGACTGGTGG CACCTGACCT GGGAGTGCTG GTGGCATCTT CCCTTTGCCT TGGCCTCTGT 
GGACGCCTCA CAAGGAAAGC CCGGCAGAGC CAGCGCACTC AGGAGCTGCA GGAGGAGGAT 
GACATAGATG CTGCCCCCGC CGCAGGGCTG CAGGGAGCCC CCACCCTGGC AACCAAACGC 
AGGCTGTGGC TGGCCCCTCG CTTCCGGATC ACGGCGCACT GGCTGCTGGT GACCTCCGGA 
CGGATGCTGG TCATCGTGCT ACTGGCACTC GCAGGCATAG CCCATCCTTC GGCCTTCTCC 
AGCGTCTACC TGATGGTGTT CCTGGCCATC TGCACCTGGT GGTCTTGCCA CTTTCCTCTC 
AGCTCCCTGG GCTTTAACAC CCTCTGCGTC ATGGTGAGCT GCTTTGGCGC CGGCCATCTT 
GTTTGTTTAT ACTGCTATCA GACACCATTT GTCCAGAGCG TGCTACTTCC TGGCAGCCTC 
TGGGCCAGGC TATTTGGTCT CAAGAACTTC GTAGACATCC CTAACTGCTC CAGCCCCAAC 
GTGCTGGTAC TCAACACTAA GCATGCTTGG CCCATCTACG TGAGCCCCGG TATCCTGCTG 
CTGTTGTACT ACACAGCCAC CTCTCTCCTG AAGCTCCGCA AGGGCCGCTT CTCAGAGCTG 
AGGAAGGAGA TCCCGAGGGA GGATGAGGAG CATGAACTGG AACTGGACCA GCTGGAGCCA 
GAGCCCCAGG CTAGGGGCAC CACCCAGGGT GCGACGCCCA CGACCACTGG ACCCGACATT 
GACAACTGCA CCGTGCATGT ACTAACCAGC CAGAGCCCCG TCCGCCAGCG TCCTGTACGC 
CCCAGGCTGG CTGAGCTGAA AGAGATGTCA CCACTACATG GCCTGGGCCA CCTCATCCTG 
GACCAGAGCT ATGTGTGTGC TCTGATCGCC ATGATGGTGT GGAGCATCAT GTACCACAGC 
TGGCTGACCT TCGTCTTGCT GCTCTGGGCC TGCCTCATCT GGACCGTGCG CAGCCGCCAC 
CAGTTGGCTA TGCTGTGCTC ACCCTGCATC CTGCTGTACG GACTGACACT CTGCTGCCTG 
CGGTACGTGT GGGCCATGGA GCTCCCCGAG CTGCCCACCA CCCTGGGCCC TGTCAGTCTA 
CATCAGTTGG GATTGGAGCA TACACGCTAC CCCTGCCTGG ACCTTGGTGC CATGCTGCTG 
TATCTGCTCA CGTTCTGGCT GCTCCTGCGT CAGTTCGTGA AGGAGAAGCT GCTGAAGAAG 
AGAAAGGCAC CTTCGACGCT GCTGGAGGTC ACAGTGTCCG ACACAGAGCC CACACAGACC 
CAGACACTGC TGAGGAGCCT GGGGGAGCTG GTCACGGGCA TCTACGTCAA GTACTGGATC 
TACGTGTGCG CTGGGATGTT CATTGTGGTC AGCTTCGCTG GCCGCCTGGT GGTCTACAAA 
ATCGTCTACA TGTTCCTTTT CCTGCTGTGC CTCACGTTGT TCCAGGTCTA CTACACTCTG 
TGGAGGAAGC TGCTCCGAGT TTTCTGGTGG CTCGTGGTGG CCTACACCAT GCTCGTGCTC 
ATCGCCGTGT ACACTTTCCA GTTCCAGGAC TTCCCCACCT ACTGGCGCAA CCTCACGGGC 
TTCACAGATG AACAGTTGGG CGACCTGGGC CTGGAGCAGT TCAGCGTGTC GGAGCTCTTT 
TCCAGCATCC TCATCCCTGG CTTCTTCCTG CTGGCCTGCA TCCTGCAGCT GCACTACTTC 
CACAGGCCGT TCATGCAGCT CACCGACCTG GAGCACGTGC CCCCGCCAGG CACACGCCGC 
CTTCGCTGGG CTCACAGGCA GGATACAGTG AGCGAGGCCC CTCTGCTTCA GCATCAGGAG 
GAGGAGGAAG TCTTCAGGGA TGACGGGCAG AGCATGGATG GGCCCCACCA GACCACACAG 
GTCCCTGAGG GTACAGCCAG CAAGTGGGGC CTGGTGGCCG ACCGGCTGCT GGACCTAGCG 
TCCAGCTTCT CCGCCGTCCT CACCCGCATC CAGGTGTTCG TACGATGTTT GCTCGAGCTT 
CACGTCTTCA AGCTGGTGGC TCTCTACACT GTCTGGGTGG CCCTGAAAGA AGTGTCCGTG 
ATGAACCTGC TGCTGGTGGT GCTGTGGGCC TTCGCCCTGC CCTACCCGCG CTTCCGGCCC 
ATGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT 
CAGCTGAAGA TTGTCAACCC ACACGAGTAC TCCAGTAACT GCACCGAGCC CTTTCCCAAC 
AATACCAACT TGCAGCCCTT GGAGATCAGC CAGTCTCTGC TGTACCGTGG CCCCGTTGAC 
CCTGCCAACT GGTTTGGGGT GCGGAAGGGT TACCCAAACT TGGGCTATAT CCAGAACCAC 
CTGCAGATCC TTCTGCTGCT GGTGTTTGAG GCTGTGGTGT ACCGGCGCCA AGAGCACTAC 
CGCCGGCAGC ACCAGCAGGC CCCTCTGCCC GCCCAGGCTT TGTGCGCTGA TGGCACCCGC 
CAGAGGCTGG ACCAGGACCT ACTCAGCTGC CTCAAATATT TCATCAACTT CTTCTTCTAC 
AAATTCGGGC TGGAGATCTG CTTCTTGATG GCCGTGAATG TCATCGGACA ACGCATGAAC 
TTCATGGTGA TCTTACACGG CTGCTGGTTG GTGGCCATCC TTACACGCCG GCGCCGTGAG 
GCCATCGCCC GCCTCTGGCC CAATTACTGT CTGTTCCTCA CGCTGTTCCT GCTGTACCAG 
TACCTGCTGT GCCTGGGCAT GCCTCCCGCA CTGTGCATCG ACTACCCGTG GCGCTGGAGC 
CAGGCCATCC CCATGAATTC TGCGCTCATC AAGTGGCTGT ACCTCCCTGA CTTCTTCAGG 
GCCCCCAACT CCACCAACCT TATCAGTGAC TTCCTCCTGC TACTCTGCGC CTCCCAACAG 
TGGCAGGTGT TCTCCGCAGA GCAAACGGAA GAATGGCAGC GCATGGCTGG CGTTAACACT 
GACCACCTGG AGCCCCTGCG TGGGGAGCCC AACCCTATCC CCAACTTCAT CCACTGCAGG 
TCCTATCTGG ATATGCTGAA GGTGGCCGTC TTCCGCTACC TGTTCTGGCT GGTGCTGGTG 
GTCGTGTTTG TCACAGGGGC CACCCGCATC AGCATCTTTG GGCTGGGGTA CCTGCTGGCC 
TGCTTCTACC TGCTGCTCTT TGGCACCACC CTGCTGCAGA AGGACACACG AGCCCAGCTC 
GTGCTGTGGG ACTGCCTCAT CCTCTACAAC GTCACCGTCA TCATCTCTAA GAATATGTTG 
TCGCTCCTGT CCTGTGTCTT CGTGGAGCAA ATGCAGAGCA ACTTCTGCTG GGTCATCCAG 
CTCTTCAGCC TCGTGTGCAC AGTCAAAGGC TACTATGACC CCAAAGAGAT GAAGACCAGG 
GACCGGGATT GCCTGCTGCC TGTGGAGGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC 
TTCTTCCTGC TCCTACAACG GCGCGTCTTT CTCAGCCACT ACTTCCTGCA CGTCAGTGCT 
GACCTGAAGG CCACGGCCCT GCAGGCCTCC AGGGGTTTTG CTCTCTACAA TGCGGCCAAC 
ATAAAGAACA TCAACTTCCA CCGTCAGACC GAGGAGAGGT CCCTGGCCCA GCTGAAAAGA 
CAGATGAAGC GCATCCGTGC CAAACAGGAG AAATACCGGC AGAGCCAGGC AAGCCGTGGT 
CAACTCCAGT CCACAGACCC TCAGGAGCCA GTCATCCACT CTGGTGACTA CTTCCTGTTT 
GAGTCGGACA GTGAGGAGGA AGAGGAGGCC CTGCCTGAGG ACCCCAGGCC TGCAGCTCAG 
AGTGCCTTCC AGATGGCATA CCAGGCATGG GTAACCAACG CCCAGACAGT GCTAAGGCAG 
CGTCGTGAAC AGGCACGGCG GGATCGGGCA GAACAGCTGG CTTCTGGAGG CGACTTGAGT 
CCTGAGGTGG AGCCGGTAGA CGTCCCAGAG AATGAGATGG CAGGCCGTAG CCATGTGATG 
CAGCGCGTGC TGAGTACCAT GCAGTTCCTG TGGGTGCTGG GCCAGGCCAC AGTGGACGGG 
CTGACGCGCT GGTTGCGTAC GTTCACGAAG GACCACCGCA CCATGAGTGA TGTGCTGTGT 
GCAGAGCGTT ACCTGCTCAC CCAGGAGCTT CTTCGGGGTG GAGAGGTACA CCGAGGGGTA 
CTGGACCAGC TTTACGTGAG CGAAGATGAG ATCGCACTGT CAGGTCCTGT GGAGAACCGT 
GATGGACCCA GCACAGCCTC TAGCGGGCTG GGAGCTGAAG AGCCTTTGAG TAGCATGACA 
GACGACACCG GCAGCCCCCT TAGCACTGGC TACAACACTC GCAGTGGTAG TGAGGAGATT 
ATCACCGACA CTGGGGGCCT CCAGGCTGGG ACCTCCCTGC ACGGCTCCCA AGAGCTTCTA 
GCCAATGCTC GTACCCGGAT GCGCACAGCC AGTGAGCTGC TGCTGGACAG GCGCCTGCGT 
ATCCCTGAGC TGGAGGAGGC CGAGCAGTTT GAGGCACAGC AGGGCCGGAC GCTGCGGCTG 
CTCAGAGCCA TGTACCAGTG TGTGGCGGCC CACTCAGAGC TTCTCTGTTA CTTCATCATC 
ATCCTTAACC ACATGGTGAC AGCCTCGGCT GCCTCCCTGG TGCTGCCTGT GCTTGTGTTC 
CTCTGGGCCA TGCTGACCAT CCCGAGGCCT AGCAAGCGCT TCTGGATGAC AGCTATCGTC 
TTCACTGAGG TCATGGTGGT CACCAAATAC TTGTTCCAGT TTGGCTTCTT CCCCTGGAAC 
AGCTACATAG TGCTTCGACG CTACGAGAAC AAGCCCTACT TCCCTCCGCG CATCCTGGGC 
CTCGAGAAAA CGGACAGCTA TATCAAGTAC GACCTGGTGC AACTCATGGC CCTCTTCTTC 
CACCGCTCGC AGTTACTGTG CTATGGCCTC TGGGACCATG AGGAGGATGG TGTCCCCAAG 
GACCATTGCA GGAGTAGCGA AAAGGACCAA GAAGCTGAGG AAGAGTCAGA GGCTAAGCTG 
GAATCGCAGC CTGAGACGGG CACTGGGCAT CCTGAGGAGC CCGTGTTGAC TGGTACCCCC 
AAGGACCACA TCCAAGGGAA AGGAAGTGTT AGGTCCAAGG ATGAGATCCA AGATCCCCCA 
GAGGACCTTA AGCCCCAGCA CAGGAGACAC ATCAGCATAC GCTTCAGGAG GAGGAAGGAG 
ACTCAAGGAC CCAAAGGAGC AGCAGTCGTG GAGGCTGAGC ACGAGGAGGG AGAGGAAGGA 
AGAGAAGCTG CAGGGAGAAA GAGGCTGCGT CGCCCTCGAG AAGGGTTGAA GATTCGGGAG 
AAAATGAAGG CAGCTGGACG CCGGCTGCAG AGCTTCTGTC TGTCACTGGC CCAGAGCTTT 
TACCAACCCC TGCGGCGTTT CTTCCATGAC ATTCTGCACA CCAAGTACCG AGCGGCCACC 
GACGTCTATG CCCTCATGTT CCTGGCTGAC ATTGTCGACA TTGTCGTCAT CATCTTCGGT 
TTTTGGGCTT TTGGGAAGCA CTCCGCGGCC ACGGACATTG CATCCTCACT GTCAGATGAC 
CAGGTGCCAC AGGCCTTCCT GTTCATGCTG CTGGTCCAGT TCGGCACCAT GGTCATAGAC 
CGCGCCCTCT ACCTGCGTAA GACTGTACTG GGCAAGCTGG CCTTTCAGGT GGTCCTGGTG 
GTGGCCATTC ACCTCTGGAT GTTTTTCATC TTACCGGCTG TCACCGAGAG GATGTTCAGA 
CAGAACGCGG TGGCCCAGCT GTGGTACTTC GTGAAGTGCA TTTACTTTGC CCTGTCCGCC 
TACCAGATCC GCTGTGGCTA CCCCACCCGT ATCTTGGGCA ACTTCCTCAC CAAGAAGTAC 
AATCATCTAA ACCTCTTCCT TTTCCAGGGG TTCCGTCTAG TGCCATTCCT GGTGGAGCTG 
CGGGCCGTCA TGGACTGGGT ATGGACTGAC ACCACGCTGT CCCTGTCCAA CTGGATGTGT 
GTGGAAGACA TCTATGCCAA CATCTTTATC ATCAAGTGCA GCCGAGAGAC AGAGAAGAAA 
TACCCCCAGC CCAAGGGACA GAAGAAGAAG AAAATCGTCA AGTATGGCAT GGGAGGTCTC 
ATCATCCTCT TCCTCATTGC CATCATCTGG TTCCCTCTGC TCTTCATGTC ACTGATCCGC 
TCTGTGGTTG GTGTCGTCAA CCAGCCCATT GATGTCACTG TTACCCTCAA GTTAGGTGGC 
TACGAGCCAC TGTTCACCAT GAGCGCCCAG CAGCCATCCA TAGTGCCATT CACTCCCGAG 
GACTATGAGG AGCTGTCCCA GCAGTTTGAC CCCTACCCAC TAGCCATGCA GTTCATTAGC 
CAGTACAGTC CTGAGGACAT CGTCACTGCA CAGATTGAGG GCAGCTCGGG GGCCCTGTGG 
CGCATCAGCC CGCCCAGCCG GGCCCAGATG AAGCACGAGC TGTACAACGG CACAGCCGAC 
ATCACACTGC GCTTCACCTG GAATTTCCAA AGGGACCTGG CCAAGGGCGG CTCTGTGGAG 
TATACCAACG AGAAGCACAC CTTGGAGCTG GCCCCCAACA GTACGGCACG AAGGCAGCTG 
GCCCAACTGC TCGAGGGCAG ACCTGACCAG TCAGTGGTCA TTCCCCACCT CTTCCCCAAG 
TACATTCGTG CACCCAATGG GCCTGAAGCC AATCCTGTGA AGCAACTGCA GCCAGACGAG 
GAAGAGGACT ACCTTGGTGT GCGTATCCAG CTGCGGAGAG AGCAAGTGGG CACAGGAACC 
TCTGGGGAGC AAGCAGGCAC AAAGGCCTCC GACTTCCTCG AGTGGTGGGT GATTGAGCTG 
CAGGACTGCC AGGCCGAGTG CAACCTGCTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC 
CCGCCTAGCC TGGGCTTCCT GGCCGGCTAC GGGATTGTGG GGCTCTACGT CTCTATCGTG 
CTGGTCGTTG GCAAGTTTGT GCGGGGCTTC TTCAGTGATA TCTCACACTC CATCATGTTC 
GAGGAGCTGC CGTGCGTGGA CCGCATCCTC AAGCTGTGCC AGGACATCTT CTTGGTACGG 
GAGACCCGAG AGCTGGAGCT GGAGGAGGAA CTCTATGCCA AGCTCATATT CCTGTACCGA 
TCACCAGAGA CCATGATCAA GTGGACGCGT GAGAAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770849.1
CDS	618..8159
Translation	MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWLPGPSRHSIPGHTGRLLRALLCLSLLFLVAHVAFQICLHTMPRLNQLLGQNCSSLWANVSQHIGVTRLDLKDIFNTTRLVAPDLGVLVASSLCLGLCGRLTRKARQSQRTQELQEEDDIDAAPAAGLQGAPTLATKRRLWLAPRFRITAHWLLVTSGRMLVIVLLALAGIAHPSAFSSVYLMVFLAICTWWSCHFPLSSLGFNTLCVMVSCFGAGHLVCLYCYQTPFVQSVLLPGSLWARLFGLKNFVDIPNCSSPNVLVLNTKHAWPIYVSPGILLLLYYTATSLLKLRKGRFSELRKEIPREDEEHELELDQLEPEPQARGTTQGATPTTTGPDIDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLHGLGHLILDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFVKEKLLKKRKAPSTLLEVTVSDTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPTYWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHVPPPGTRRLRWAHRQDTVSEAPLLQHQEEEEVFRDDGQSMDGPHQTTQVPEGTASKWGLVADRLLDLASSFSAVLTRIQVFVRCLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEISQSLLYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQALCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSQAIPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAEQTEEWQRMAGVNTDHLEPLRGEPNPIPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTTLLQKDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYDPKEMKTRDRDCLLPVEEAGIIWDSICFFFLLLQRRVFLSHYFLHVSADLKATALQASRGFALYNAANIKNINFHRQTEERSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSTDPQEPVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQMAYQAWVTNAQTVLRQRREQARRDRAEQLASGGDLSPEVEPVDVPENEMAGRSHVMQRVLSTMQFLWVLGQATVDGLTRWLRTFTKDHRTMSDVLCAERYLLTQELLRGGEVHRGVLDQLYVSEDEIALSGPVENRDGPSTASSGLGAEEPLSSMTDDTGSPLSTGYNTRSGSEEIITDTGGLQAGTSLHGSQELLANARTRMRTASELLLDRRLRIPELEEAEQFEAQQGRTLRLLRAMYQCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVMVVTKYLFQFGFFPWNSYIVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQLLCYGLWDHEEDGVPKDHCRSSEKDQEAEEESEAKLESQPETGTGHPEEPVLTGTPKDHIQGKGSVRSKDEIQDPPEDLKPQHRRHISIRFRRRKETQGPKGAAVVEAEHEEGEEGREAAGRKRLRRPREGLKIREKMKAAGRRLQSFCLSLAQSFYQPLRRFFHDILHTKYRAATDVYALMFLADIVDIVVIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLRKTVLGKLAFQVVLVVAIHLWMFFILPAVTERMFRQNAVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPEDYEELSQQFDPYPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKHELYNGTADITLRFTWNFQRDLAKGGSVEYTNEKHTLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLGVRIQLRREQVGTGTSGEQAGTKASDFLEWWVIELQDCQAECNLLPMVIFSDKVSPPSLGFLAGYGIVGLYVSIVLVVGKFVRGFFSDISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_008772627.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772627.2

ORF Insert Sequence:

ATGGAGCCGC ACGTGCTGGG CGCCGGGCTC TACTGGCTGT TGCTACCCTG CACACTCCTG 
GCGGCCTCCC TGTTACGCTT CAATGCTCTC TCGTTGGTCT ATTTGCTGTT TCTACTGCTG 
CTACCCTGGC TTCCAGGCCC CTCAAGACAC AGCATACCAG GTCACACAGG TCGCCTGCTT 
CGTGCACTAC TCTGCCTCAG CCTCCTCTTC CTGGTGGCCC ATGTTGCCTT CCAGATATGC 
CTACATACCA TGCCTCGCCT GAACCAGCTT CTGGGACAAA ACTGCAGCAG CCTTTGGGCA 
AACGTGTCTC AACACATAGG GGTTACAAGG CTGGACCTGA AGGACATCTT TAACACCACC 
AGACTGGTGG CACCTGACCT GGGAGTGCTG GTGGCATCTT CCCTTTGCCT TGGCCTCTGT 
GGACGCCTCA CAAGGAAAGC CCGGCAGAGC CAGCGCACTC AGGAGCTGCA GGAGGAGGAT 
GACATAGATG CTGCCCCCGC CGCAGGGCTG CAGGGAGCCC CCACCCTGGC AACCAAACGC 
AGGCTGTGGC TGGCCCCTCG CTTCCGGATC ACGGCGCACT GGCTGCTGGT GACCTCCGGA 
CGGATGCTGG TCATCGTGCT ACTGGCACTC GCAGGCATAG CCCATCCTTC GGCCTTCTCC 
AGCGTCTACC TGATGGTGTT CCTGGCCATC TGCACCTGGT GGTCTTGCCA CTTTCCTCTC 
AGCTCCCTGG GCTTTAACAC CCTCTGCGTC ATGGTGAGCT GCTTTGGCGC CGGCCATCTT 
GTTTGTTTAT ACTGCTATCA GACACCATTT GTCCAGAGCG TGCTACTTCC TGGCAGCCTC 
TGGGCCAGGC TATTTGGTCT CAAGAACTTC GTAGACATCC CTAACTGCTC CAGCCCCAAC 
GTGCTGGTAC TCAACACTAA GCATGCTTGG CCCATCTACG TGAGCCCCGG TATCCTGCTG 
CTGTTGTACT ACACAGCCAC CTCTCTCCTG AAGCTCCGCA AGGGCCGCTT CTCAGAGCTG 
AGGAAGGAGA TCCCGAGGGA GGATGAGGAG CATGAACTGG AACTGGACCA GCTGGAGCCA 
GAGCCCCAGG CTAGGGGCAC CACCCAGGGT GCGACGCCCA CGACCACTGG ACCCGACATT 
GACAACTGCA CCGTGCATGT ACTAACCAGC CAGAGCCCCG TCCGCCAGCG TCCTGTACGC 
CCCAGGCTGG CTGAGCTGAA AGAGATGTCA CCACTACATG GCCTGGGCCA CCTCATCCTG 
GACCAGAGCT ATGTGTGTGC TCTGATCGCC ATGATGGTGT GGAGCATCAT GTACCACAGC 
TGGCTGACCT TCGTCTTGCT GCTCTGGGCC TGCCTCATCT GGACCGTGCG CAGCCGCCAC 
CAGTTGGCTA TGCTGTGCTC ACCCTGCATC CTGCTGTACG GACTGACACT CTGCTGCCTG 
CGGTACGTGT GGGCCATGGA GCTCCCCGAG CTGCCCACCA CCCTGGGCCC TGTCAGTCTA 
CATCAGTTGG GATTGGAGCA TACACGCTAC CCCTGCCTGG ACCTTGGTGC CATGCTGCTG 
TATCTGCTCA CGTTCTGGCT GCTCCTGCGT CAGTTCGTGA AGGAGAAGCT GCTGAAGAAG 
AGAAAGGCAC CTTCGACGCT GCTGGAGGTC ACAGTGTCCG ACACAGAGCC CACACAGACC 
CAGACACTGC TGAGGAGCCT GGGGGAGCTG GTCACGGGCA TCTACGTCAA GTACTGGATC 
TACGTGTGCG CTGGGATGTT CATTGTGGTC AGCTTCGCTG GCCGCCTGGT GGTCTACAAA 
ATCGTCTACA TGTTCCTTTT CCTGCTGTGC CTCACGTTGT TCCAGGTCTA CTACACTCTG 
TGGAGGAAGC TGCTCCGAGT TTTCTGGTGG CTCGTGGTGG CCTACACCAT GCTCGTGCTC 
ATCGCCGTGT ACACTTTCCA GTTCCAGGAC TTCCCCACCT ACTGGCGCAA CCTCACGGGC 
TTCACAGATG AACAGTTGGG CGACCTGGGC CTGGAGCAGT TCAGCGTGTC GGAGCTCTTT 
TCCAGCATCC TCATCCCTGG CTTCTTCCTG CTGGCCTGCA TCCTGCAGCT GCACTACTTC 
CACAGGCCGT TCATGCAGCT CACCGACCTG GAGCACGTGC CCCCGCCAGG CACACGCCGC 
CTTCGCTGGG CTCACAGGCA GGATACAGTG AGCGAGGCCC CTCTGCTTCA GCATCAGGAG 
GAGGAGGAAG TCTTCAGGGA TGACGGGCAG AGCATGGATG GGCCCCACCA GACCACACAG 
GTCCCTGAGG GTACAGCCAG CAAGTGGGGC CTGGTGGCCG ACCGGCTGCT GGACCTAGCG 
TCCAGCTTCT CCGCCGTCCT CACCCGCATC CAGGTGTTCG TACGATGTTT GCTCGAGCTT 
CACGTCTTCA AGCTGGTGGC TCTCTACACT GTCTGGGTGG CCCTGAAAGA AGTGTCCGTG 
ATGAACCTGC TGCTGGTGGT GCTGTGGGCC TTCGCCCTGC CCTACCCGCG CTTCCGGCCC 
ATGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT 
CAGCTGAAGA TTGTCAACCC ACACGAGTAC TCCAGTAACT GCACCGAGCC CTTTCCCAAC 
AATACCAACT TGCAGCCCTT GGAGATCAGC CAGTCTCTGC TGTACCGTGG CCCCGTTGAC 
CCTGCCAACT GGTTTGGGGT GCGGAAGGGT TACCCAAACT TGGGCTATAT CCAGAACCAC 
CTGCAGATCC TTCTGCTGCT GGTGTTTGAG GCTGTGGTGT ACCGGCGCCA AGAGCACTAC 
CGCCGGCAGC ACCAGCAGGC CCCTCTGCCC GCCCAGGCTT TGTGCGCTGA TGGCACCCGC 
CAGAGGCTGG ACCAGGACCT ACTCAGCTGC CTCAAATATT TCATCAACTT CTTCTTCTAC 
AAATTCGGGC TGGAGATCTG CTTCTTGATG GCCGTGAATG TCATCGGACA ACGCATGAAC 
TTCATGGTGA TCTTACACGG CTGCTGGTTG GTGGCCATCC TTACACGCCG GCGCCGTGAG 
GCCATCGCCC GCCTCTGGCC CAATTACTGT CTGTTCCTCA CGCTGTTCCT GCTGTACCAG 
TACCTGCTGT GCCTGGGCAT GCCTCCCGCA CTGTGCATCG ACTACCCGTG GCGCTGGAGC 
CAGGCCATCC CCATGAATTC TGCGCTCATC AAGTGGCTGT ACCTCCCTGA CTTCTTCAGG 
GCCCCCAACT CCACCAACCT TATCAGTGAC TTCCTCCTGC TACTCTGCGC CTCCCAACAG 
TGGCAGGTGT TCTCCGCAGA GCAAACGGAA GAATGGCAGC GCATGGCTGG CGTTAACACT 
GACCACCTGG AGCCCCTGCG TGGGGAGCCC AACCCTATCC CCAACTTCAT CCACTGCAGG 
TCCTATCTGG ATATGCTGAA GGTGGCCGTC TTCCGCTACC TGTTCTGGCT GGTGCTGGTG 
GTCGTGTTTG TCACAGGGGC CACCCGCATC AGCATCTTTG GGCTGGGGTA CCTGCTGGCC 
TGCTTCTACC TGCTGCTCTT TGGCACCACC CTGCTGCAGA AGGACACACG AGCCCAGCTC 
GTGCTGTGGG ACTGCCTCAT CCTCTACAAC GTCACCGTCA TCATCTCTAA GAATATGTTG 
TCGCTCCTGT CCTGTGTCTT CGTGGAGCAA ATGCAGAGCA ACTTCTGCTG GGTCATCCAG 
CTCTTCAGCC TCGTGTGCAC AGTCAAAGGC TACTATGACC CCAAAGAGAT GAAGACCAGG 
GACCGGGATT GCCTGCTGCC TGTGGAGGAG GCCGGGATCA TCTGGGACAG CATCTGCTTC 
TTCTTCCTGC TCCTACAACG GCGCGTCTTT CTCAGCCACT ACTTCCTGCA CGTCAGTGCT 
GACCTGAAGG CCACGGCCCT GCAGGCCTCC AGGGGTTTTG CTCTCTACAA TGCGGCCAAC 
ATAAAGAACA TCAACTTCCA CCGTCAGACC GAGGAGAGGT CCCTGGCCCA GCTGAAAAGA 
CAGATGAAGC GCATCCGTGC CAAACAGGAG AAATACCGGC AGAGCCAGGC AAGCCGTGGT 
CAACTCCAGT CCACAGACCC TCAGGAGCCA GTCATCCACT CTGGTGACTA CTTCCTGTTT 
GAGTCGGACA GTGAGGAGGA AGAGGAGGCC CTGCCTGAGG ACCCCAGGCC TGCAGCTCAG 
AGTGCCTTCC AGATGGCATA CCAGGCATGG GTAACCAACG CCCAGACAGT GCTAAGGCAG 
CGTCGTGAAC AGGCACGGCG GGATCGGGCA GAACAGCTGG CTTCTGGAGG CGACTTGAGT 
CCTGAGGTGG AGCCGGTAGA CGTCCCAGAG AATGAGATGG CAGGCCGTAG CCATGTGATG 
CAGCGCGTGC TGAGTACCAT GCAGTTCCTG TGGGTGCTGG GCCAGGCCAC AGTGGACGGG 
CTGACGCGCT GGTTGCGTAC GTTCACGAAG GACCACCGCA CCATGAGTGA TGTGCTGTGT 
GCAGAGCGTT ACCTGCTCAC CCAGGAGCTT CTTCGGGGTG GAGAGGTACA CCGAGGGGTA 
CTGGACCAGC TTTACGTGAG CGAAGATGAG ATCGCACTGT CAGGTCCTGT GGAGAACCGT 
GATGGACCCA GCACAGCCTC TAGCGGGCTG GGAGCTGAAG AGCCTTTGAG TAGCATGACA 
GACGACACCG GCAGCCCCCT TAGCACTGGC TACAACACTC GCAGTGGTAG TGAGGAGATT 
ATCACCGACA CTGGGGGCCT CCAGGCTGGG ACCTCCCTGC ACGGCTCCCA AGAGCTTCTA 
GCCAATGCTC GTACCCGGAT GCGCACAGCC AGTGAGCTGC TGCTGGACAG GCGCCTGCGT 
ATCCCTGAGC TGGAGGAGGC CGAGCAGTTT GAGGCACAGC AGGGCCGGAC GCTGCGGCTG 
CTCAGAGCCA TGTACCAGTG TGTGGCGGCC CACTCAGAGC TTCTCTGTTA CTTCATCATC 
ATCCTTAACC ACATGGTGAC AGCCTCGGCT GCCTCCCTGG TGCTGCCTGT GCTTGTGTTC 
CTCTGGGCCA TGCTGACCAT CCCGAGGCCT AGCAAGCGCT TCTGGATGAC AGCTATCGTC 
TTCACTGAGG TCATGGTGGT CACCAAATAC TTGTTCCAGT TTGGCTTCTT CCCCTGGAAC 
AGCTACATAG TGCTTCGACG CTACGAGAAC AAGCCCTACT TCCCTCCGCG CATCCTGGGC 
CTCGAGAAAA CGGACAGCTA TATCAAGTAC GACCTGGTGC AACTCATGGC CCTCTTCTTC 
CACCGCTCGC AGTTACTGTG CTATGGCCTC TGGGACCATG AGGAGGATGG TGTCCCCAAG 
GACCATTGCA GGAGTAGCGA AAAGGACCAA GAAGCTGAGG AAGAGTCAGA GGCTAAGCTG 
GAATCGCAGC CTGAGACGGG CACTGGGCAT CCTGAGGAGC CCGTGTTGAC TGGTACCCCC 
AAGGACCACA TCCAAGGGAA AGGAAGTGTT AGGTCCAAGG ATGAGATCCA AGATCCCCCA 
GAGGACCTTA AGCCCCAGCA CAGGAGACAC ATCAGCATAC GCTTCAGGAG GAGGAAGGAG 
ACTCAAGGAC CCAAAGGAGC AGCAGTCGTG GAGGCTGAGC ACGAGGAGGG AGAGGAAGGA 
AGAGAAGCTG CAGGGAGAAA GAGGCTGCGT CGCCCTCGAG AAGGGTTGAA GATTCGGGAG 
AAAATGAAGG CAGCTGGACG CCGGCTGCAG AGCTTCTGTC TGTCACTGGC CCAGAGCTTT 
TACCAACCCC TGCGGCGTTT CTTCCATGAC ATTCTGCACA CCAAGTACCG AGCGGCCACC 
GACGTCTATG CCCTCATGTT CCTGGCTGAC ATTGTCGACA TTGTCGTCAT CATCTTCGGT 
TTTTGGGCTT TTGGGAAGCA CTCCGCGGCC ACGGACATTG CATCCTCACT GTCAGATGAC 
CAGGTGCCAC AGGCCTTCCT GTTCATGCTG CTGGTCCAGT TCGGCACCAT GGTCATAGAC 
CGCGCCCTCT ACCTGCGTAA GACTGTACTG GGCAAGCTGG CCTTTCAGGT GGTCCTGGTG 
GTGGCCATTC ACCTCTGGAT GTTTTTCATC TTACCGGCTG TCACCGAGAG GATGTTCAGA 
CAGAACGCGG TGGCCCAGCT GTGGTACTTC GTGAAGTGCA TTTACTTTGC CCTGTCCGCC 
TACCAGATCC GCTGTGGCTA CCCCACCCGT ATCTTGGGCA ACTTCCTCAC CAAGAAGTAC 
AATCATCTAA ACCTCTTCCT TTTCCAGGGG TTCCGTCTAG TGCCATTCCT GGTGGAGCTG 
CGGGCCGTCA TGGACTGGGT ATGGACTGAC ACCACGCTGT CCCTGTCCAA CTGGATGTGT 
GTGGAAGACA TCTATGCCAA CATCTTTATC ATCAAGTGCA GCCGAGAGAC AGAGAAGAAA 
TACCCCCAGC CCAAGGGACA GAAGAAGAAG AAAATCGTCA AGTATGGCAT GGGAGGTCTC 
ATCATCCTCT TCCTCATTGC CATCATCTGG TTCCCTCTGC TCTTCATGTC ACTGATCCGC 
TCTGTGGTTG GTGTCGTCAA CCAGCCCATT GATGTCACTG TTACCCTCAA GTTAGGTGGC 
TACGAGCCAC TGTTCACCAT GAGCGCCCAG CAGCCATCCA TAGTGCCATT CACTCCCGAG 
GACTATGAGG AGCTGTCCCA GCAGTTTGAC CCCTACCCAC TAGCCATGCA GTTCATTAGC 
CAGTACAGTC CTGAGGACAT CGTCACTGCA CAGATTGAGG GCAGCTCGGG GGCCCTGTGG 
CGCATCAGCC CGCCCAGCCG GGCCCAGATG AAGCACGAGC TGTACAACGG CACAGCCGAC 
ATCACACTGC GCTTCACCTG GAATTTCCAA AGGGACCTGG CCAAGGGCGG CTCTGTGGAG 
TATACCAACG AGAAGCACAC CTTGGAGCTG GCCCCCAACA GTACGGCACG AAGGCAGCTG 
GCCCAACTGC TCGAGGGCAG ACCTGACCAG TCAGTGGTCA TTCCCCACCT CTTCCCCAAG 
TACATTCGTG CACCCAATGG GCCTGAAGCC AATCCTGTGA AGCAACTGCA GCCAGACGAG 
GAAGAGGACT ACCTTGGTGT GCGTATCCAG CTGCGGAGAG AGCAAGTGGG CACAGGAACC 
TCTGGGGAGC AAGCAGGCAC AAAGGCCTCC GACTTCCTCG AGTGGTGGGT GATTGAGCTG 
CAGGACTGCC AGGCCGAGTG CAACCTGCTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC 
CCGCCTAGCC TGGGCTTCCT GGCCGGCTAC GGGATTGTGG GGCTCTACGT CTCTATCGTG 
CTGGTCGTTG GCAAGTTTGT GCGGGGCTTC TTCAGTGATA TCTCACACTC CATCATGTTC 
GAGGAGCTGC CGTGCGTGGA CCGCATCCTC AAGCTGTGCC AGGACATCTT CTTGGTACGG 
GAGACCCGAG AGCTGGAGCT GGAGGAGGAA CTCTATGCCA AGCTCATATT CCTGTACCGA 
TCACCAGAGA CCATGATCAA GTGGACGCGT GAGAAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Piezo1 ( XM_017601325.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456814.1 Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA.

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CloneID	ORa47914
Clone ID Related Accession (Same CDS sequence)	XM_017601325.1
Accession Version	XM_017601325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	piezo-type mechanosensitive ion channel component 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGCC TGAACCAGCT TCTGGGACAA AACTGCAGCA GCCTTTGGGC AAACGTGTCT 
CAACACATAG GGGTTACAAG GCTGGACCTG AAGGACATCT TTAACACCAC CAGACTGGTG 
GCACCTGACC TGGGAGTGCT GGTGGCATCT TCCCTTTGCC TTGGCCTCTG TGGACGCCTC 
ACAAGGAAAG CCCGGCAGAG CCAGCGCACT CAGGAGCTGC AGGAGGAGGA TGACATAGAT 
GCTGCCCCCG CCGCAGGGCT GCAGGGAGCC CCCACCCTGG CAACCAAACG CAGGCTGTGG 
CTGGCCCCTC GCTTCCGGAT CACGGCGCAC TGGCTGCTGG TGACCTCCGG ACGGATGCTG 
GTCATCGTGC TACTGGCACT CGCAGGCATA GCCCATCCTT CGGCCTTCTC CAGCGTCTAC 
CTGATGGTGT TCCTGGCCAT CTGCACCTGG TGGTCTTGCC ACTTTCCTCT CAGCTCCCTG 
GGCTTTAACA CCCTCTGCGT CATGGTGAGC TGCTTTGGCG CCGGCCATCT TGTTTGTTTA 
TACTGCTATC AGACACCATT TGTCCAGAGC GTGCTACTTC CTGGCAGCCT CTGGGCCAGG 
CTATTTGGTC TCAAGAACTT CGTAGACATC CCTAACTGCT CCAGCCCCAA CGTGCTGGTA 
CTCAACACTA AGCATGCTTG GCCCATCTAC GTGAGCCCCG GTATCCTGCT GCTGTTGTAC 
TACACAGCCA CCTCTCTCCT GAAGCTCCGC AAGGGCCGCT TCTCAGAGCT GAGGAAGGAG 
ATCCCGAGGG AGGATGAGGA GCATGAACTG GAACTGGACC AGCTGGAGCC AGAGCCCCAG 
GCTAGGGGCA CCACCCAGGG TGCGACGCCC ACGACCACTG GACCCGACAT TGACAACTGC 
ACCGTGCATG TACTAACCAG CCAGAGCCCC GTCCGCCAGC GTCCTGTACG CCCCAGGCTG 
GCTGAGCTGA AAGAGATGTC ACCACTACAT GGCCTGGGCC ACCTCATCCT GGACCAGAGC 
TATGTGTGTG CTCTGATCGC CATGATGGTG TGGAGCATCA TGTACCACAG CTGGCTGACC 
TTCGTCTTGC TGCTCTGGGC CTGCCTCATC TGGACCGTGC GCAGCCGCCA CCAGTTGGCT 
ATGCTGTGCT CACCCTGCAT CCTGCTGTAC GGACTGACAC TCTGCTGCCT GCGGTACGTG 
TGGGCCATGG AGCTCCCCGA GCTGCCCACC ACCCTGGGCC CTGTCAGTCT ACATCAGTTG 
GGATTGGAGC ATACACGCTA CCCCTGCCTG GACCTTGGTG CCATGCTGCT GTATCTGCTC 
ACGTTCTGGC TGCTCCTGCG TCAGTTCGTG AAGGAGAAGC TGCTGAAGAA GAGAAAGGCA 
CCTTCGACGC TGCTGGAGGT CACAGTGTCC GACACAGAGC CCACACAGAC CCAGACACTG 
CTGAGGAGCC TGGGGGAGCT GGTCACGGGC ATCTACGTCA AGTACTGGAT CTACGTGTGC 
GCTGGGATGT TCATTGTGGT CAGCTTCGCT GGCCGCCTGG TGGTCTACAA AATCGTCTAC 
ATGTTCCTTT TCCTGCTGTG CCTCACGTTG TTCCAGGTCT ACTACACTCT GTGGAGGAAG 
CTGCTCCGAG TTTTCTGGTG GCTCGTGGTG GCCTACACCA TGCTCGTGCT CATCGCCGTG 
TACACTTTCC AGTTCCAGGA CTTCCCCACC TACTGGCGCA ACCTCACGGG CTTCACAGAT 
GAACAGTTGG GCGACCTGGG CCTGGAGCAG TTCAGCGTGT CGGAGCTCTT TTCCAGCATC 
CTCATCCCTG GCTTCTTCCT GCTGGCCTGC ATCCTGCAGC TGCACTACTT CCACAGGCCG 
TTCATGCAGC TCACCGACCT GGAGCACGTG CCCCCGCCAG GCACACGCCG CCTTCGCTGG 
GCTCACAGGC AGGATACAGT GAGCGAGGCC CCTCTGCTTC AGCATCAGGA GGAGGAGGAA 
GTCTTCAGGG ATGACGGGCA GAGCATGGAT GGGCCCCACC AGACCACACA GGTCCCTGAG 
GGTACAGCCA GCAAGTGGGG CCTGGTGGCC GACCGGCTGC TGGACCTAGC GTCCAGCTTC 
TCCGCCGTCC TCACCCGCAT CCAGGTGTTC GTACGATGTT TGCTCGAGCT TCACGTCTTC 
AAGCTGGTGG CTCTCTACAC TGTCTGGGTG GCCCTGAAAG AAGTGTCCGT GATGAACCTG 
CTGCTGGTGG TGCTGTGGGC CTTCGCCCTG CCCTACCCGC GCTTCCGGCC CATGGCCTCC 
TGCCTGTCCA CCGTGTGGAC CTGTATCATC ATCGTGTGCA AGATGCTGTA TCAGCTGAAG 
ATTGTCAACC CACACGAGTA CTCCAGTAAC TGCACCGAGC CCTTTCCCAA CAATACCAAC 
TTGCAGCCCT TGGAGATCAG CCAGTCTCTG CTGTACCGTG GCCCCGTTGA CCCTGCCAAC 
TGGTTTGGGG TGCGGAAGGG TTACCCAAAC TTGGGCTATA TCCAGAACCA CCTGCAGATC 
CTTCTGCTGC TGGTGTTTGA GGCTGTGGTG TACCGGCGCC AAGAGCACTA CCGCCGGCAG 
CACCAGCAGG CCCCTCTGCC CGCCCAGGCT TTGTGCGCTG ATGGCACCCG CCAGAGGCTG 
GACCAGGACC TACTCAGCTG CCTCAAATAT TTCATCAACT TCTTCTTCTA CAAATTCGGG 
CTGGAGATCT GCTTCTTGAT GGCCGTGAAT GTCATCGGAC AACGCATGAA CTTCATGGTG 
ATCTTACACG GCTGCTGGTT GGTGGCCATC CTTACACGCC GGCGCCGTGA GGCCATCGCC 
CGCCTCTGGC CCAATTACTG TCTGTTCCTC ACGCTGTTCC TGCTGTACCA GTACCTGCTG 
TGCCTGGGCA TGCCTCCCGC ACTGTGCATC GACTACCCGT GGCGCTGGAG CCAGGCCATC 
CCCATGAATT CTGCGCTCAT CAAGTGGCTG TACCTCCCTG ACTTCTTCAG GGCCCCCAAC 
TCCACCAACC TTATCAGTGA CTTCCTCCTG CTACTCTGCG CCTCCCAACA GTGGCAGGTG 
TTCTCCGCAG AGCAAACGGA AGAATGGCAG CGCATGGCTG GCGTTAACAC TGACCACCTG 
GAGCCCCTGC GTGGGGAGCC CAACCCTATC CCCAACTTCA TCCACTGCAG GTCCTATCTG 
GATATGCTGA AGGTGGCCGT CTTCCGCTAC CTGTTCTGGC TGGTGCTGGT GGTCGTGTTT 
GTCACAGGGG CCACCCGCAT CAGCATCTTT GGGCTGGGGT ACCTGCTGGC CTGCTTCTAC 
CTGCTGCTCT TTGGCACCAC CCTGCTGCAG AAGGACACAC GAGCCCAGCT CGTGCTGTGG 
GACTGCCTCA TCCTCTACAA CGTCACCGTC ATCATCTCTA AGAATATGTT GTCGCTCCTG 
TCCTGTGTCT TCGTGGAGCA AATGCAGAGC AACTTCTGCT GGGTCATCCA GCTCTTCAGC 
CTCGTGTGCA CAGTCAAAGG CTACTATGAC CCCAAAGAGA TGAAGACCAG GGACCGGGAT 
TGCCTGCTGC CTGTGGAGGA GGCCGGGATC ATCTGGGACA GCATCTGCTT CTTCTTCCTG 
CTCCTACAAC GGCGCGTCTT TCTCAGCCAC TACTTCCTGC ACGTCAGTGC TGACCTGAAG 
GCCACGGCCC TGCAGGCCTC CAGGGGTTTT GCTCTCTACA ATGCGGCCAA CATAAAGAAC 
ATCAACTTCC ACCGTCAGAC CGAGGAGAGG TCCCTGGCCC AGCTGAAAAG ACAGATGAAG 
CGCATCCGTG CCAAACAGGA GAAATACCGG CAGAGCCAGG CAAGCCGTGG TCAACTCCAG 
TCCACAGACC CTCAGGAGCC AGGGCCTGAC AGCCCAGGGG GTTCCTCCCC GCCACGGACA 
CAGTGGTGGC GCCCCTGGCA GGACCACGCC ACAGTCATCC ACTCTGGTGA CTACTTCCTG 
TTTGAGTCGG ACAGTGAGGA GGAAGAGGAG GCCCTGCCTG AGGACCCCAG GCCTGCAGCT 
CAGAGTGCCT TCCAGATGGC ATACCAGGCA TGGGTAACCA ACGCCCAGAC AGTGCTAAGG 
CAGCGTCGTG AACAGGCACG GCGGGATCGG GCAGAACAGC TGGCTTCTGG AGGCGACTTG 
AGTCCTGAGG TGGAGCCGGT AGACGTCCCA GAGAATGAGA TGGCAGGCCG TAGCCATGTG 
ATGCAGCGCG TGCTGAGTAC CATGCAGTTC CTGTGGGTGC TGGGCCAGGC CACAGTGGAC 
GGGCTGACGC GCTGGTTGCG TACGTTCACG AAGGACCACC GCACCATGAG TGATGTGCTG 
TGTGCAGAGC GTTACCTGCT CACCCAGGAG CTTCTTCGGG GTGGAGAGGT ACACCGAGGG 
GTACTGGACC AGCTTTACGT GAGCGAAGAT GAGATCGCAC TGTCAGGTCC TGTGGAGAAC 
CGTGATGGAC CCAGCACAGC CTCTAGCGGG CTGGGAGCTG AAGAGCCTTT GAGTAGCATG 
ACAGACGACA CCGGCAGCCC CCTTAGCACT GGCTACAACA CTCGCAGTGG TAGTGAGGAG 
ATTATCACCG ACACTGGGGG CCTCCAGGCT GGGACCTCCC TGCACGGCTC CCAAGAGCTT 
CTAGCCAATG CTCGTACCCG GATGCGCACA GCCAGTGAGC TGCTGCTGGA CAGGCGCCTG 
CGTATCCCTG AGCTGGAGGA GGCCGAGCAG TTTGAGGCAC AGCAGGGCCG GACGCTGCGG 
CTGCTCAGAG CCATGTACCA GTGTGTGGCG GCCCACTCAG AGCTTCTCTG TTACTTCATC 
ATCATCCTTA ACCACATGGT GACAGCCTCG GCTGCCTCCC TGGTGCTGCC TGTGCTTGTG 
TTCCTCTGGG CCATGCTGAC CATCCCGAGG CCTAGCAAGC GCTTCTGGAT GACAGCTATC 
GTCTTCACTG AGGTCATGGT GGTCACCAAA TACTTGTTCC AGTTTGGCTT CTTCCCCTGG 
AACAGCTACA TAGTGCTTCG ACGCTACGAG AACAAGCCCT ACTTCCCTCC GCGCATCCTG 
GGCCTCGAGA AAACGGACAG CTATATCAAG TACGACCTGG TGCAACTCAT GGCCCTCTTC 
TTCCACCGCT CGCAGTTACT GTGCTATGGC CTCTGGGACC ATGAGGAGGA TGGTGTCCCC 
AAGGACCATT GCAGGAGTAG CGAAAAGGAC CAAGAAGCTG AGGAAGAGTC AGAGGCTAAG 
CTGGAATCGC AGCCTGAGAC GGGCACTGGG CATCCTGAGG AGCCCGTGTT GACTGGTACC 
CCCAAGGACC ACATCCAAGG GAAAGGAAGT GTTAGGTCCA AGGATGAGAT CCAAGATCCC 
CCAGAGGACC TTAAGCCCCA GCACAGGAGA CACATCAGCA TACGCTTCAG GAGGAGGAAG 
GAGACTCAAG GACCCAAAGG AGCAGCAGTC GTGGAGGCTG AGCACGAGGA GGGAGAGGAA 
GGAAGAGAAG CTGCAGGGAG AAAGAGGCTG CGTCGCCCTC GAGAAGGGTT GAAGATTCGG 
GAGAAAATGA AGGCAGCTGG ACGCCGGCTG CAGAGCTTCT GTCTGTCACT GGCCCAGAGC 
TTTTACCAAC CCCTGCGGCG TTTCTTCCAT GACATTCTGC ACACCAAGTA CCGAGCGGCC 
ACCGACGTCT ATGCCCTCAT GTTCCTGGCT GACATTGTCG ACATTGTCGT CATCATCTTC 
GGTTTTTGGG CTTTTGGGAA GCACTCCGCG GCCACGGACA TTGCATCCTC ACTGTCAGAT 
GACCAGGTGC CACAGGCCTT CCTGTTCATG CTGCTGGTCC AGTTCGGCAC CATGGTCATA 
GACCGCGCCC TCTACCTGCG TAAGACTGTA CTGGGCAAGC TGGCCTTTCA GGTGGTCCTG 
GTGGTGGCCA TTCACCTCTG GATGTTTTTC ATCTTACCGG CTGTCACCGA GAGGATGTTC 
AGACAGAACG CGGTGGCCCA GCTGTGGTAC TTCGTGAAGT GCATTTACTT TGCCCTGTCC 
GCCTACCAGA TCCGCTGTGG CTACCCCACC CGTATCTTGG GCAACTTCCT CACCAAGAAG 
TACAATCATC TAAACCTCTT CCTTTTCCAG GGGTTCCGTC TAGTGCCATT CCTGGTGGAG 
CTGCGGGCCG TCATGGACTG GGTATGGACT GACACCACGC TGTCCCTGTC CAACTGGATG 
TGTGTGGAAG ACATCTATGC CAACATCTTT ATCATCAAGT GCAGCCGAGA GACAGAGAAG 
AAATACCCCC AGCCCAAGGG ACAGAAGAAG AAGAAAATCG TCAAGTATGG CATGGGAGGT 
CTCATCATCC TCTTCCTCAT TGCCATCATC TGGTTCCCTC TGCTCTTCAT GTCACTGATC 
CGCTCTGTGG TTGGTGTCGT CAACCAGCCC ATTGATGTCA CTGTTACCCT CAAGTTAGGT 
GGCTACGAGC CACTGTTCAC CATGAGCGCC CAGCAGCCAT CCATAGTGCC ATTCACTCCC 
GAGGACTATG AGGAGCTGTC CCAGCAGTTT GACCCCTACC CACTAGCCAT GCAGTTCATT 
AGCCAGTACA GTCCTGAGGA CATCGTCACT GCACAGATTG AGGGCAGCTC GGGGGCCCTG 
TGGCGCATCA GCCCGCCCAG CCGGGCCCAG ATGAAGCACG AGCTGTACAA CGGCACAGCC 
GACATCACAC TGCGCTTCAC CTGGAATTTC CAAAGGGACC TGGCCAAGGG CGGCTCTGTG 
GAGTATACCA ACGAGAAGCA CACCTTGGAG CTGGCCCCCA ACAGTACGGC ACGAAGGCAG 
CTGGCCCAAC TGCTCGAGGG CAGACCTGAC CAGTCAGTGG TCATTCCCCA CCTCTTCCCC 
AAGTACATTC GTGCACCCAA TGGGCCTGAA GCCAATCCTG TGAAGCAACT GCAGCCAGAC 
GAGGAAGAGG ACTACCTTGG TGTGCGTATC CAGCTGCGGA GAGAGCAAGT GGGCACAGGA 
ACCTCTGGGG AGCAAGCAGG CACAAAGGCC TCCGACTTCC TCGAGTGGTG GGTGATTGAG 
CTGCAGGACT GCCAGGCCGA GTGCAACCTG CTGCCCATGG TCATCTTCAG TGACAAGGTC 
AGCCCGCCTA GCCTGGGCTT CCTGGCCGGC TACGGGATTG TGGGGCTCTA CGTCTCTATC 
GTGCTGGTCG TTGGCAAGTT TGTGCGGGGC TTCTTCAGTG ATATCTCACA CTCCATCATG 
TTCGAGGAGC TGCCGTGCGT GGACCGCATC CTCAAGCTGT GCCAGGACAT CTTCTTGGTA 
CGGGAGACCC GAGAGCTGGA GCTGGAGGAG GAACTCTATG CCAAGCTCAT ATTCCTGTAC 
CGATCACCAG AGACCATGAT CAAGTGGACG CGTGAGAAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456814.1
CDS	6322..13686
Translation	MPRLNQLLGQNCSSLWANVSQHIGVTRLDLKDIFNTTRLVAPDLGVLVASSLCLGLCGRLTRKARQSQRTQELQEEDDIDAAPAAGLQGAPTLATKRRLWLAPRFRITAHWLLVTSGRMLVIVLLALAGIAHPSAFSSVYLMVFLAICTWWSCHFPLSSLGFNTLCVMVSCFGAGHLVCLYCYQTPFVQSVLLPGSLWARLFGLKNFVDIPNCSSPNVLVLNTKHAWPIYVSPGILLLLYYTATSLLKLRKGRFSELRKEIPREDEEHELELDQLEPEPQARGTTQGATPTTTGPDIDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLHGLGHLILDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFVKEKLLKKRKAPSTLLEVTVSDTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPTYWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHVPPPGTRRLRWAHRQDTVSEAPLLQHQEEEEVFRDDGQSMDGPHQTTQVPEGTASKWGLVADRLLDLASSFSAVLTRIQVFVRCLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEISQSLLYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQALCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSQAIPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAEQTEEWQRMAGVNTDHLEPLRGEPNPIPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTTLLQKDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYDPKEMKTRDRDCLLPVEEAGIIWDSICFFFLLLQRRVFLSHYFLHVSADLKATALQASRGFALYNAANIKNINFHRQTEERSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSTDPQEPGPDSPGGSSPPRTQWWRPWQDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQMAYQAWVTNAQTVLRQRREQARRDRAEQLASGGDLSPEVEPVDVPENEMAGRSHVMQRVLSTMQFLWVLGQATVDGLTRWLRTFTKDHRTMSDVLCAERYLLTQELLRGGEVHRGVLDQLYVSEDEIALSGPVENRDGPSTASSGLGAEEPLSSMTDDTGSPLSTGYNTRSGSEEIITDTGGLQAGTSLHGSQELLANARTRMRTASELLLDRRLRIPELEEAEQFEAQQGRTLRLLRAMYQCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVMVVTKYLFQFGFFPWNSYIVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQLLCYGLWDHEEDGVPKDHCRSSEKDQEAEEESEAKLESQPETGTGHPEEPVLTGTPKDHIQGKGSVRSKDEIQDPPEDLKPQHRRHISIRFRRRKETQGPKGAAVVEAEHEEGEEGREAAGRKRLRRPREGLKIREKMKAAGRRLQSFCLSLAQSFYQPLRRFFHDILHTKYRAATDVYALMFLADIVDIVVIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLRKTVLGKLAFQVVLVVAIHLWMFFILPAVTERMFRQNAVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPEDYEELSQQFDPYPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKHELYNGTADITLRFTWNFQRDLAKGGSVEYTNEKHTLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLGVRIQLRREQVGTGTSGEQAGTKASDFLEWWVIELQDCQAECNLLPMVIFSDKVSPPSLGFLAGYGIVGLYVSIVLVVGKFVRGFFSDISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_017601325.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus piezo-type mechanosensitive ion channel component 1 (Piezo1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601325.1

ORF Insert Sequence:

ATGCCTCGCC TGAACCAGCT TCTGGGACAA AACTGCAGCA GCCTTTGGGC AAACGTGTCT 
CAACACATAG GGGTTACAAG GCTGGACCTG AAGGACATCT TTAACACCAC CAGACTGGTG 
GCACCTGACC TGGGAGTGCT GGTGGCATCT TCCCTTTGCC TTGGCCTCTG TGGACGCCTC 
ACAAGGAAAG CCCGGCAGAG CCAGCGCACT CAGGAGCTGC AGGAGGAGGA TGACATAGAT 
GCTGCCCCCG CCGCAGGGCT GCAGGGAGCC CCCACCCTGG CAACCAAACG CAGGCTGTGG 
CTGGCCCCTC GCTTCCGGAT CACGGCGCAC TGGCTGCTGG TGACCTCCGG ACGGATGCTG 
GTCATCGTGC TACTGGCACT CGCAGGCATA GCCCATCCTT CGGCCTTCTC CAGCGTCTAC 
CTGATGGTGT TCCTGGCCAT CTGCACCTGG TGGTCTTGCC ACTTTCCTCT CAGCTCCCTG 
GGCTTTAACA CCCTCTGCGT CATGGTGAGC TGCTTTGGCG CCGGCCATCT TGTTTGTTTA 
TACTGCTATC AGACACCATT TGTCCAGAGC GTGCTACTTC CTGGCAGCCT CTGGGCCAGG 
CTATTTGGTC TCAAGAACTT CGTAGACATC CCTAACTGCT CCAGCCCCAA CGTGCTGGTA 
CTCAACACTA AGCATGCTTG GCCCATCTAC GTGAGCCCCG GTATCCTGCT GCTGTTGTAC 
TACACAGCCA CCTCTCTCCT GAAGCTCCGC AAGGGCCGCT TCTCAGAGCT GAGGAAGGAG 
ATCCCGAGGG AGGATGAGGA GCATGAACTG GAACTGGACC AGCTGGAGCC AGAGCCCCAG 
GCTAGGGGCA CCACCCAGGG TGCGACGCCC ACGACCACTG GACCCGACAT TGACAACTGC 
ACCGTGCATG TACTAACCAG CCAGAGCCCC GTCCGCCAGC GTCCTGTACG CCCCAGGCTG 
GCTGAGCTGA AAGAGATGTC ACCACTACAT GGCCTGGGCC ACCTCATCCT GGACCAGAGC 
TATGTGTGTG CTCTGATCGC CATGATGGTG TGGAGCATCA TGTACCACAG CTGGCTGACC 
TTCGTCTTGC TGCTCTGGGC CTGCCTCATC TGGACCGTGC GCAGCCGCCA CCAGTTGGCT 
ATGCTGTGCT CACCCTGCAT CCTGCTGTAC GGACTGACAC TCTGCTGCCT GCGGTACGTG 
TGGGCCATGG AGCTCCCCGA GCTGCCCACC ACCCTGGGCC CTGTCAGTCT ACATCAGTTG 
GGATTGGAGC ATACACGCTA CCCCTGCCTG GACCTTGGTG CCATGCTGCT GTATCTGCTC 
ACGTTCTGGC TGCTCCTGCG TCAGTTCGTG AAGGAGAAGC TGCTGAAGAA GAGAAAGGCA 
CCTTCGACGC TGCTGGAGGT CACAGTGTCC GACACAGAGC CCACACAGAC CCAGACACTG 
CTGAGGAGCC TGGGGGAGCT GGTCACGGGC ATCTACGTCA AGTACTGGAT CTACGTGTGC 
GCTGGGATGT TCATTGTGGT CAGCTTCGCT GGCCGCCTGG TGGTCTACAA AATCGTCTAC 
ATGTTCCTTT TCCTGCTGTG CCTCACGTTG TTCCAGGTCT ACTACACTCT GTGGAGGAAG 
CTGCTCCGAG TTTTCTGGTG GCTCGTGGTG GCCTACACCA TGCTCGTGCT CATCGCCGTG 
TACACTTTCC AGTTCCAGGA CTTCCCCACC TACTGGCGCA ACCTCACGGG CTTCACAGAT 
GAACAGTTGG GCGACCTGGG CCTGGAGCAG TTCAGCGTGT CGGAGCTCTT TTCCAGCATC 
CTCATCCCTG GCTTCTTCCT GCTGGCCTGC ATCCTGCAGC TGCACTACTT CCACAGGCCG 
TTCATGCAGC TCACCGACCT GGAGCACGTG CCCCCGCCAG GCACACGCCG CCTTCGCTGG 
GCTCACAGGC AGGATACAGT GAGCGAGGCC CCTCTGCTTC AGCATCAGGA GGAGGAGGAA 
GTCTTCAGGG ATGACGGGCA GAGCATGGAT GGGCCCCACC AGACCACACA GGTCCCTGAG 
GGTACAGCCA GCAAGTGGGG CCTGGTGGCC GACCGGCTGC TGGACCTAGC GTCCAGCTTC 
TCCGCCGTCC TCACCCGCAT CCAGGTGTTC GTACGATGTT TGCTCGAGCT TCACGTCTTC 
AAGCTGGTGG CTCTCTACAC TGTCTGGGTG GCCCTGAAAG AAGTGTCCGT GATGAACCTG 
CTGCTGGTGG TGCTGTGGGC CTTCGCCCTG CCCTACCCGC GCTTCCGGCC CATGGCCTCC 
TGCCTGTCCA CCGTGTGGAC CTGTATCATC ATCGTGTGCA AGATGCTGTA TCAGCTGAAG 
ATTGTCAACC CACACGAGTA CTCCAGTAAC TGCACCGAGC CCTTTCCCAA CAATACCAAC 
TTGCAGCCCT TGGAGATCAG CCAGTCTCTG CTGTACCGTG GCCCCGTTGA CCCTGCCAAC 
TGGTTTGGGG TGCGGAAGGG TTACCCAAAC TTGGGCTATA TCCAGAACCA CCTGCAGATC 
CTTCTGCTGC TGGTGTTTGA GGCTGTGGTG TACCGGCGCC AAGAGCACTA CCGCCGGCAG 
CACCAGCAGG CCCCTCTGCC CGCCCAGGCT TTGTGCGCTG ATGGCACCCG CCAGAGGCTG 
GACCAGGACC TACTCAGCTG CCTCAAATAT TTCATCAACT TCTTCTTCTA CAAATTCGGG 
CTGGAGATCT GCTTCTTGAT GGCCGTGAAT GTCATCGGAC AACGCATGAA CTTCATGGTG 
ATCTTACACG GCTGCTGGTT GGTGGCCATC CTTACACGCC GGCGCCGTGA GGCCATCGCC 
CGCCTCTGGC CCAATTACTG TCTGTTCCTC ACGCTGTTCC TGCTGTACCA GTACCTGCTG 
TGCCTGGGCA TGCCTCCCGC ACTGTGCATC GACTACCCGT GGCGCTGGAG CCAGGCCATC 
CCCATGAATT CTGCGCTCAT CAAGTGGCTG TACCTCCCTG ACTTCTTCAG GGCCCCCAAC 
TCCACCAACC TTATCAGTGA CTTCCTCCTG CTACTCTGCG CCTCCCAACA GTGGCAGGTG 
TTCTCCGCAG AGCAAACGGA AGAATGGCAG CGCATGGCTG GCGTTAACAC TGACCACCTG 
GAGCCCCTGC GTGGGGAGCC CAACCCTATC CCCAACTTCA TCCACTGCAG GTCCTATCTG 
GATATGCTGA AGGTGGCCGT CTTCCGCTAC CTGTTCTGGC TGGTGCTGGT GGTCGTGTTT 
GTCACAGGGG CCACCCGCAT CAGCATCTTT GGGCTGGGGT ACCTGCTGGC CTGCTTCTAC 
CTGCTGCTCT TTGGCACCAC CCTGCTGCAG AAGGACACAC GAGCCCAGCT CGTGCTGTGG 
GACTGCCTCA TCCTCTACAA CGTCACCGTC ATCATCTCTA AGAATATGTT GTCGCTCCTG 
TCCTGTGTCT TCGTGGAGCA AATGCAGAGC AACTTCTGCT GGGTCATCCA GCTCTTCAGC 
CTCGTGTGCA CAGTCAAAGG CTACTATGAC CCCAAAGAGA TGAAGACCAG GGACCGGGAT 
TGCCTGCTGC CTGTGGAGGA GGCCGGGATC ATCTGGGACA GCATCTGCTT CTTCTTCCTG 
CTCCTACAAC GGCGCGTCTT TCTCAGCCAC TACTTCCTGC ACGTCAGTGC TGACCTGAAG 
GCCACGGCCC TGCAGGCCTC CAGGGGTTTT GCTCTCTACA ATGCGGCCAA CATAAAGAAC 
ATCAACTTCC ACCGTCAGAC CGAGGAGAGG TCCCTGGCCC AGCTGAAAAG ACAGATGAAG 
CGCATCCGTG CCAAACAGGA GAAATACCGG CAGAGCCAGG CAAGCCGTGG TCAACTCCAG 
TCCACAGACC CTCAGGAGCC AGGGCCTGAC AGCCCAGGGG GTTCCTCCCC GCCACGGACA 
CAGTGGTGGC GCCCCTGGCA GGACCACGCC ACAGTCATCC ACTCTGGTGA CTACTTCCTG 
TTTGAGTCGG ACAGTGAGGA GGAAGAGGAG GCCCTGCCTG AGGACCCCAG GCCTGCAGCT 
CAGAGTGCCT TCCAGATGGC ATACCAGGCA TGGGTAACCA ACGCCCAGAC AGTGCTAAGG 
CAGCGTCGTG AACAGGCACG GCGGGATCGG GCAGAACAGC TGGCTTCTGG AGGCGACTTG 
AGTCCTGAGG TGGAGCCGGT AGACGTCCCA GAGAATGAGA TGGCAGGCCG TAGCCATGTG 
ATGCAGCGCG TGCTGAGTAC CATGCAGTTC CTGTGGGTGC TGGGCCAGGC CACAGTGGAC 
GGGCTGACGC GCTGGTTGCG TACGTTCACG AAGGACCACC GCACCATGAG TGATGTGCTG 
TGTGCAGAGC GTTACCTGCT CACCCAGGAG CTTCTTCGGG GTGGAGAGGT ACACCGAGGG 
GTACTGGACC AGCTTTACGT GAGCGAAGAT GAGATCGCAC TGTCAGGTCC TGTGGAGAAC 
CGTGATGGAC CCAGCACAGC CTCTAGCGGG CTGGGAGCTG AAGAGCCTTT GAGTAGCATG 
ACAGACGACA CCGGCAGCCC CCTTAGCACT GGCTACAACA CTCGCAGTGG TAGTGAGGAG 
ATTATCACCG ACACTGGGGG CCTCCAGGCT GGGACCTCCC TGCACGGCTC CCAAGAGCTT 
CTAGCCAATG CTCGTACCCG GATGCGCACA GCCAGTGAGC TGCTGCTGGA CAGGCGCCTG 
CGTATCCCTG AGCTGGAGGA GGCCGAGCAG TTTGAGGCAC AGCAGGGCCG GACGCTGCGG 
CTGCTCAGAG CCATGTACCA GTGTGTGGCG GCCCACTCAG AGCTTCTCTG TTACTTCATC 
ATCATCCTTA ACCACATGGT GACAGCCTCG GCTGCCTCCC TGGTGCTGCC TGTGCTTGTG 
TTCCTCTGGG CCATGCTGAC CATCCCGAGG CCTAGCAAGC GCTTCTGGAT GACAGCTATC 
GTCTTCACTG AGGTCATGGT GGTCACCAAA TACTTGTTCC AGTTTGGCTT CTTCCCCTGG 
AACAGCTACA TAGTGCTTCG ACGCTACGAG AACAAGCCCT ACTTCCCTCC GCGCATCCTG 
GGCCTCGAGA AAACGGACAG CTATATCAAG TACGACCTGG TGCAACTCAT GGCCCTCTTC 
TTCCACCGCT CGCAGTTACT GTGCTATGGC CTCTGGGACC ATGAGGAGGA TGGTGTCCCC 
AAGGACCATT GCAGGAGTAG CGAAAAGGAC CAAGAAGCTG AGGAAGAGTC AGAGGCTAAG 
CTGGAATCGC AGCCTGAGAC GGGCACTGGG CATCCTGAGG AGCCCGTGTT GACTGGTACC 
CCCAAGGACC ACATCCAAGG GAAAGGAAGT GTTAGGTCCA AGGATGAGAT CCAAGATCCC 
CCAGAGGACC TTAAGCCCCA GCACAGGAGA CACATCAGCA TACGCTTCAG GAGGAGGAAG 
GAGACTCAAG GACCCAAAGG AGCAGCAGTC GTGGAGGCTG AGCACGAGGA GGGAGAGGAA 
GGAAGAGAAG CTGCAGGGAG AAAGAGGCTG CGTCGCCCTC GAGAAGGGTT GAAGATTCGG 
GAGAAAATGA AGGCAGCTGG ACGCCGGCTG CAGAGCTTCT GTCTGTCACT GGCCCAGAGC 
TTTTACCAAC CCCTGCGGCG TTTCTTCCAT GACATTCTGC ACACCAAGTA CCGAGCGGCC 
ACCGACGTCT ATGCCCTCAT GTTCCTGGCT GACATTGTCG ACATTGTCGT CATCATCTTC 
GGTTTTTGGG CTTTTGGGAA GCACTCCGCG GCCACGGACA TTGCATCCTC ACTGTCAGAT 
GACCAGGTGC CACAGGCCTT CCTGTTCATG CTGCTGGTCC AGTTCGGCAC CATGGTCATA 
GACCGCGCCC TCTACCTGCG TAAGACTGTA CTGGGCAAGC TGGCCTTTCA GGTGGTCCTG 
GTGGTGGCCA TTCACCTCTG GATGTTTTTC ATCTTACCGG CTGTCACCGA GAGGATGTTC 
AGACAGAACG CGGTGGCCCA GCTGTGGTAC TTCGTGAAGT GCATTTACTT TGCCCTGTCC 
GCCTACCAGA TCCGCTGTGG CTACCCCACC CGTATCTTGG GCAACTTCCT CACCAAGAAG 
TACAATCATC TAAACCTCTT CCTTTTCCAG GGGTTCCGTC TAGTGCCATT CCTGGTGGAG 
CTGCGGGCCG TCATGGACTG GGTATGGACT GACACCACGC TGTCCCTGTC CAACTGGATG 
TGTGTGGAAG ACATCTATGC CAACATCTTT ATCATCAAGT GCAGCCGAGA GACAGAGAAG 
AAATACCCCC AGCCCAAGGG ACAGAAGAAG AAGAAAATCG TCAAGTATGG CATGGGAGGT 
CTCATCATCC TCTTCCTCAT TGCCATCATC TGGTTCCCTC TGCTCTTCAT GTCACTGATC 
CGCTCTGTGG TTGGTGTCGT CAACCAGCCC ATTGATGTCA CTGTTACCCT CAAGTTAGGT 
GGCTACGAGC CACTGTTCAC CATGAGCGCC CAGCAGCCAT CCATAGTGCC ATTCACTCCC 
GAGGACTATG AGGAGCTGTC CCAGCAGTTT GACCCCTACC CACTAGCCAT GCAGTTCATT 
AGCCAGTACA GTCCTGAGGA CATCGTCACT GCACAGATTG AGGGCAGCTC GGGGGCCCTG 
TGGCGCATCA GCCCGCCCAG CCGGGCCCAG ATGAAGCACG AGCTGTACAA CGGCACAGCC 
GACATCACAC TGCGCTTCAC CTGGAATTTC CAAAGGGACC TGGCCAAGGG CGGCTCTGTG 
GAGTATACCA ACGAGAAGCA CACCTTGGAG CTGGCCCCCA ACAGTACGGC ACGAAGGCAG 
CTGGCCCAAC TGCTCGAGGG CAGACCTGAC CAGTCAGTGG TCATTCCCCA CCTCTTCCCC 
AAGTACATTC GTGCACCCAA TGGGCCTGAA GCCAATCCTG TGAAGCAACT GCAGCCAGAC 
GAGGAAGAGG ACTACCTTGG TGTGCGTATC CAGCTGCGGA GAGAGCAAGT GGGCACAGGA 
ACCTCTGGGG AGCAAGCAGG CACAAAGGCC TCCGACTTCC TCGAGTGGTG GGTGATTGAG 
CTGCAGGACT GCCAGGCCGA GTGCAACCTG CTGCCCATGG TCATCTTCAG TGACAAGGTC 
AGCCCGCCTA GCCTGGGCTT CCTGGCCGGC TACGGGATTG TGGGGCTCTA CGTCTCTATC 
GTGCTGGTCG TTGGCAAGTT TGTGCGGGGC TTCTTCAGTG ATATCTCACA CTCCATCATG 
TTCGAGGAGC TGCCGTGCGT GGACCGCATC CTCAAGCTGT GCCAGGACAT CTTCTTGGTA 
CGGGAGACCC GAGAGCTGGA GCTGGAGGAG GAACTCTATG CCAAGCTCAT ATTCCTGTAC 
CGATCACCAG AGACCATGAT CAAGTGGACG CGTGAGAAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Piezo1 cation channel mediates uterine artery shear stress mechanotransduction and vasodilation during rat pregnancy.
American journal of physiology. Heart and circulatory physiology315(4)H1019-H1026(2018 10)
John L,Ko NL,Gokin A,Gokina N,Mandalà M,Osol G

Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis.
Experimental & molecular medicine50(5)60(2018 05)
Liu Q,Sun B,Zhao J,Wang Q,An F,Hu X,Yang Z,Xu J,Tan M,Li L

Structure and mechanogating mechanism of the Piezo1 channel.
Nature554(7693)487-492(2018 02)
Zhao Q,Zhou H,Chi S,Wang Y,Wang J,Geng J,Wu K,Liu W,Zhang T,Dong MQ,Wang J,Li X,Xiao B

Structure of the mechanically activated ion channel Piezo1.
Nature554(7693)481-486(2018 02)
Saotome K,Murthy SE,Kefauver JM,Whitwam T,Patapoutian A,Ward AB

Piezo1 expression increases in rat bladder after partial bladder outlet obstruction.
Life sciences1661-7(2016 Dec)
Michishita M,Yano K,Tomita KI,Matsuzaki O,Kasahara KI

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Piezo1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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