Sema6a gene cDNA ORF clone, Rattus norvegicus(Norway rat)

mRNA	Protein	Name
NM_001108430.2	NP_001101900.2	semaphorin-6A isoform 1 precursor
XM_008772186.1	XP_008770408.1	semaphorin-6A isoform X1
XM_006222572.3	XP_006222634.1	semaphorin-6A isoform X2
XM_003751782.4	XP_003751830.1	semaphorin-6A isoform X1
XM_006254704.3	XP_006254766.1	semaphorin-6A isoform X3
XM_008774127.2	XP_008772349.1	semaphorin-6A isoform X1
XM_003753046.4	XP_003753094.1	semaphorin-6A isoform X1
XM_006222573.3	XP_006222635.1	semaphorin-6A isoform X3
XM_006254703.3	XP_006254765.1	semaphorin-6A isoform X2
NM_001372354.1	NP_001359283.1	semaphorin-6A isoform 2 precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa20884	NM_001372354.1 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa20884	XM_006254704.3 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa20884	XM_006222573.3 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa20883	NM_001108430.2 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa20882	XM_003753046.4 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa20882	XM_008774127.2 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa20882	XM_003751782.4 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa20882	XM_008772186.1 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa20883	XM_006222572.3 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa20883	XM_006254703.3 Latest version!	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

Sema6a ( NM_001372354.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001359283.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa20884
Clone ID Related Accession (Same CDS sequence)	XM_006254704.3 , XM_006222573.3 , NM_001372354.1
Accession Version	NM_001372354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1566921600000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07031972.1, AABR07031971.1 and CK839311.1. On or before Aug 29, 2019 this sequence version replaced XM_006254704.3, XM_006222573.3. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. The extent of this transcript is supported by orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG AGTGATTCGG 
GAAAGTTACC TCAAAAGCAA CGACCAGCTC GTTCCTGTCA CCCTTTTGGC CATTGCAGTC 
ATTCTGGCTT TTGTCATGGG GGCAGTCTTC TCCGGCATCA TCGTGTATTG TGTGTGTGAT 
CACCGGCGCA AAGACGTGGC GGTAGTGCAA CGCAAGGAGA AAGAGCTCAC CCACTCGCGT 
CGGGGATCTA TGAGCAGTGT CACCAAGCTC AGTGGCCTCT TTGGGGACAG TCAGTCCAAG 
GACCCAAAGC CTGAGGCCAT CCTCACACCA CTCATGCACA ATGGCAAACT GGCCACGCCT 
AGCAACACCG CCAAGATGCT CATCAAGGCT GACCAGCATC ACTTAGACCT CACCGCCCTG 
CCCACCCCGG AGTCCACCCC AACACTGCAG CAGAAGCGGA AACCCAACCG GGGCAGTCGC 
GAGTGGGAGA GGAACCAGAA CCTCATCAAT GCCTGCACCA AGGACATGCC TCCCATGGGC 
TCCCCTGTGA TTCCCACGGA CCTGCCTCTC CGGGCCTCCC CAAGCCACAT CCCCAGCGTG 
GTGGTCCTGC CCATCACACA GCAGGGCTAC CAGCACGAAT ACGTAGATCA GCCCAAAATG 
AGCGAGGTGG TGGCTCAGAT GGCACTGGAG GACCAGGCTG CCACCCTGGA GTATAAGACC 
ATCAAAGAGC ACCTGAGCAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG 
GACAGCCTGC CCCCCAAAGT TCCACAGCGT GAGGCCTCTC TGGGTCCCAC GGGAGCCTCA 
CTGTCCCAGA CCGGCCTGAG CAAGCGGCTG GAAATGCAAC ACTCCTCCTC CTACGGGCTT 
GAATATAAGA GGAGCTACCC CACGAACTCG CTCACAAGAA GCCATCAGGC CACCACTCTC 
AAAAGAAACA ATACTAACTC CTCCAATTCC TCCCACCTCT CCAGGAACCA GAGCCTTGGC 
CGGGGAGACA ACCCACCTCC CGCCCCGCAG AGGGTGGACT CTATCCAGGT GCACAGCCCC 
CAGCCCTCTG GCCAGGCCGT GACTGTTTCG AGGCAGCCCA GCCTCAATGC CTACAACTCA 
CTGACGAGGT CGGGGCTGAA GCGCACCCCC TCGCTAAAGC CAGATGTACC CCCCAAACCT 
TCCTTTGCCC CCCTTTCCAC ATCCATGAAG CCCAATGATG CGTGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001359283.1
CDS	970..3900
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	NM_001372354.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372354.1

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG AGTGATTCGG 
GAAAGTTACC TCAAAAGCAA CGACCAGCTC GTTCCTGTCA CCCTTTTGGC CATTGCAGTC 
ATTCTGGCTT TTGTCATGGG GGCAGTCTTC TCCGGCATCA TCGTGTATTG TGTGTGTGAT 
CACCGGCGCA AAGACGTGGC GGTAGTGCAA CGCAAGGAGA AAGAGCTCAC CCACTCGCGT 
CGGGGATCTA TGAGCAGTGT CACCAAGCTC AGTGGCCTCT TTGGGGACAG TCAGTCCAAG 
GACCCAAAGC CTGAGGCCAT CCTCACACCA CTCATGCACA ATGGCAAACT GGCCACGCCT 
AGCAACACCG CCAAGATGCT CATCAAGGCT GACCAGCATC ACTTAGACCT CACCGCCCTG 
CCCACCCCGG AGTCCACCCC AACACTGCAG CAGAAGCGGA AACCCAACCG GGGCAGTCGC 
GAGTGGGAGA GGAACCAGAA CCTCATCAAT GCCTGCACCA AGGACATGCC TCCCATGGGC 
TCCCCTGTGA TTCCCACGGA CCTGCCTCTC CGGGCCTCCC CAAGCCACAT CCCCAGCGTG 
GTGGTCCTGC CCATCACACA GCAGGGCTAC CAGCACGAAT ACGTAGATCA GCCCAAAATG 
AGCGAGGTGG TGGCTCAGAT GGCACTGGAG GACCAGGCTG CCACCCTGGA GTATAAGACC 
ATCAAAGAGC ACCTGAGCAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG 
GACAGCCTGC CCCCCAAAGT TCCACAGCGT GAGGCCTCTC TGGGTCCCAC GGGAGCCTCA 
CTGTCCCAGA CCGGCCTGAG CAAGCGGCTG GAAATGCAAC ACTCCTCCTC CTACGGGCTT 
GAATATAAGA GGAGCTACCC CACGAACTCG CTCACAAGAA GCCATCAGGC CACCACTCTC 
AAAAGAAACA ATACTAACTC CTCCAATTCC TCCCACCTCT CCAGGAACCA GAGCCTTGGC 
CGGGGAGACA ACCCACCTCC CGCCCCGCAG AGGGTGGACT CTATCCAGGT GCACAGCCCC 
CAGCCCTCTG GCCAGGCCGT GACTGTTTCG AGGCAGCCCA GCCTCAATGC CTACAACTCA 
CTGACGAGGT CGGGGCTGAA GCGCACCCCC TCGCTAAAGC CAGATGTACC CCCCAAACCT 
TCCTTTGCCC CCCTTTCCAC ATCCATGAAG CCCAATGATG CGTGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_006254704.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254766.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa20884
Clone ID Related Accession (Same CDS sequence)	XM_006254704.3 , XM_006222573.3 , NM_001372354.1
Accession Version	XM_006254704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG AGTGATTCGG 
GAAAGTTACC TCAAAAGCAA CGACCAGCTC GTTCCTGTCA CCCTTTTGGC CATTGCAGTC 
ATTCTGGCTT TTGTCATGGG GGCAGTCTTC TCCGGCATCA TCGTGTATTG TGTGTGTGAT 
CACCGGCGCA AAGACGTGGC GGTAGTGCAA CGCAAGGAGA AAGAGCTCAC CCACTCGCGT 
CGGGGATCTA TGAGCAGTGT CACCAAGCTC AGTGGCCTCT TTGGGGACAG TCAGTCCAAG 
GACCCAAAGC CTGAGGCCAT CCTCACACCA CTCATGCACA ATGGCAAACT GGCCACGCCT 
AGCAACACCG CCAAGATGCT CATCAAGGCT GACCAGCATC ACTTAGACCT CACCGCCCTG 
CCCACCCCGG AGTCCACCCC AACACTGCAG CAGAAGCGGA AACCCAACCG GGGCAGTCGC 
GAGTGGGAGA GGAACCAGAA CCTCATCAAT GCCTGCACCA AGGACATGCC TCCCATGGGC 
TCCCCTGTGA TTCCCACGGA CCTGCCTCTC CGGGCCTCCC CAAGCCACAT CCCCAGCGTG 
GTGGTCCTGC CCATCACACA GCAGGGCTAC CAGCACGAAT ACGTAGATCA GCCCAAAATG 
AGCGAGGTGG TGGCTCAGAT GGCACTGGAG GACCAGGCTG CCACCCTGGA GTATAAGACC 
ATCAAAGAGC ACCTGAGCAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG 
GACAGCCTGC CCCCCAAAGT TCCACAGCGT GAGGCCTCTC TGGGTCCCAC GGGAGCCTCA 
CTGTCCCAGA CCGGCCTGAG CAAGCGGCTG GAAATGCAAC ACTCCTCCTC CTACGGGCTT 
GAATATAAGA GGAGCTACCC CACGAACTCG CTCACAAGAA GCCATCAGGC CACCACTCTC 
AAAAGAAACA ATACTAACTC CTCCAATTCC TCCCACCTCT CCAGGAACCA GAGCCTTGGC 
CGGGGAGACA ACCCACCTCC CGCCCCGCAG AGGGTGGACT CTATCCAGGT GCACAGCCCC 
CAGCCCTCTG GCCAGGCCGT GACTGTTTCG AGGCAGCCCA GCCTCAATGC CTACAACTCA 
CTGACGAGGT CGGGGCTGAA GCGCACCCCC TCGCTAAAGC CAGATGTACC CCCCAAACCT 
TCCTTTGCCC CCCTTTCCAC ATCCATGAAG CCCAATGATG CGTGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254766.1
CDS	966..3896
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_006254704.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254704.3

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG AGTGATTCGG 
GAAAGTTACC TCAAAAGCAA CGACCAGCTC GTTCCTGTCA CCCTTTTGGC CATTGCAGTC 
ATTCTGGCTT TTGTCATGGG GGCAGTCTTC TCCGGCATCA TCGTGTATTG TGTGTGTGAT 
CACCGGCGCA AAGACGTGGC GGTAGTGCAA CGCAAGGAGA AAGAGCTCAC CCACTCGCGT 
CGGGGATCTA TGAGCAGTGT CACCAAGCTC AGTGGCCTCT TTGGGGACAG TCAGTCCAAG 
GACCCAAAGC CTGAGGCCAT CCTCACACCA CTCATGCACA ATGGCAAACT GGCCACGCCT 
AGCAACACCG CCAAGATGCT CATCAAGGCT GACCAGCATC ACTTAGACCT CACCGCCCTG 
CCCACCCCGG AGTCCACCCC AACACTGCAG CAGAAGCGGA AACCCAACCG GGGCAGTCGC 
GAGTGGGAGA GGAACCAGAA CCTCATCAAT GCCTGCACCA AGGACATGCC TCCCATGGGC 
TCCCCTGTGA TTCCCACGGA CCTGCCTCTC CGGGCCTCCC CAAGCCACAT CCCCAGCGTG 
GTGGTCCTGC CCATCACACA GCAGGGCTAC CAGCACGAAT ACGTAGATCA GCCCAAAATG 
AGCGAGGTGG TGGCTCAGAT GGCACTGGAG GACCAGGCTG CCACCCTGGA GTATAAGACC 
ATCAAAGAGC ACCTGAGCAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG 
GACAGCCTGC CCCCCAAAGT TCCACAGCGT GAGGCCTCTC TGGGTCCCAC GGGAGCCTCA 
CTGTCCCAGA CCGGCCTGAG CAAGCGGCTG GAAATGCAAC ACTCCTCCTC CTACGGGCTT 
GAATATAAGA GGAGCTACCC CACGAACTCG CTCACAAGAA GCCATCAGGC CACCACTCTC 
AAAAGAAACA ATACTAACTC CTCCAATTCC TCCCACCTCT CCAGGAACCA GAGCCTTGGC 
CGGGGAGACA ACCCACCTCC CGCCCCGCAG AGGGTGGACT CTATCCAGGT GCACAGCCCC 
CAGCCCTCTG GCCAGGCCGT GACTGTTTCG AGGCAGCCCA GCCTCAATGC CTACAACTCA 
CTGACGAGGT CGGGGCTGAA GCGCACCCCC TCGCTAAAGC CAGATGTACC CCCCAAACCT 
TCCTTTGCCC CCCTTTCCAC ATCCATGAAG CCCAATGATG CGTGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_006222573.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006222635.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X4, mRNA.

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CloneID	ORa20884
Clone ID Related Accession (Same CDS sequence)	XM_006254704.3 , XM_006222573.3 , NM_001372354.1
Accession Version	XM_006222573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006222573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG AGTGATTCGG 
GAAAGTTACC TCAAAAGCAA CGACCAGCTC GTTCCTGTCA CCCTTTTGGC CATTGCAGTC 
ATTCTGGCTT TTGTCATGGG GGCAGTCTTC TCCGGCATCA TCGTGTATTG TGTGTGTGAT 
CACCGGCGCA AAGACGTGGC GGTAGTGCAA CGCAAGGAGA AAGAGCTCAC CCACTCGCGT 
CGGGGATCTA TGAGCAGTGT CACCAAGCTC AGTGGCCTCT TTGGGGACAG TCAGTCCAAG 
GACCCAAAGC CTGAGGCCAT CCTCACACCA CTCATGCACA ATGGCAAACT GGCCACGCCT 
AGCAACACCG CCAAGATGCT CATCAAGGCT GACCAGCATC ACTTAGACCT CACCGCCCTG 
CCCACCCCGG AGTCCACCCC AACACTGCAG CAGAAGCGGA AACCCAACCG GGGCAGTCGC 
GAGTGGGAGA GGAACCAGAA CCTCATCAAT GCCTGCACCA AGGACATGCC TCCCATGGGC 
TCCCCTGTGA TTCCCACGGA CCTGCCTCTC CGGGCCTCCC CAAGCCACAT CCCCAGCGTG 
GTGGTCCTGC CCATCACACA GCAGGGCTAC CAGCACGAAT ACGTAGATCA GCCCAAAATG 
AGCGAGGTGG TGGCTCAGAT GGCACTGGAG GACCAGGCTG CCACCCTGGA GTATAAGACC 
ATCAAAGAGC ACCTGAGCAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG 
GACAGCCTGC CCCCCAAAGT TCCACAGCGT GAGGCCTCTC TGGGTCCCAC GGGAGCCTCA 
CTGTCCCAGA CCGGCCTGAG CAAGCGGCTG GAAATGCAAC ACTCCTCCTC CTACGGGCTT 
GAATATAAGA GGAGCTACCC CACGAACTCG CTCACAAGAA GCCATCAGGC CACCACTCTC 
AAAAGAAACA ATACTAACTC CTCCAATTCC TCCCACCTCT CCAGGAACCA GAGCCTTGGC 
CGGGGAGACA ACCCACCTCC CGCCCCGCAG AGGGTGGACT CTATCCAGGT GCACAGCCCC 
CAGCCCTCTG GCCAGGCCGT GACTGTTTCG AGGCAGCCCA GCCTCAATGC CTACAACTCA 
CTGACGAGGT CGGGGCTGAA GCGCACCCCC TCGCTAAAGC CAGATGTACC CCCCAAACCT 
TCCTTTGCCC CCCTTTCCAC ATCCATGAAG CCCAATGATG CGTGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006222635.1
CDS	966..3896
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_006222573.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006222573.3

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG AGTGATTCGG 
GAAAGTTACC TCAAAAGCAA CGACCAGCTC GTTCCTGTCA CCCTTTTGGC CATTGCAGTC 
ATTCTGGCTT TTGTCATGGG GGCAGTCTTC TCCGGCATCA TCGTGTATTG TGTGTGTGAT 
CACCGGCGCA AAGACGTGGC GGTAGTGCAA CGCAAGGAGA AAGAGCTCAC CCACTCGCGT 
CGGGGATCTA TGAGCAGTGT CACCAAGCTC AGTGGCCTCT TTGGGGACAG TCAGTCCAAG 
GACCCAAAGC CTGAGGCCAT CCTCACACCA CTCATGCACA ATGGCAAACT GGCCACGCCT 
AGCAACACCG CCAAGATGCT CATCAAGGCT GACCAGCATC ACTTAGACCT CACCGCCCTG 
CCCACCCCGG AGTCCACCCC AACACTGCAG CAGAAGCGGA AACCCAACCG GGGCAGTCGC 
GAGTGGGAGA GGAACCAGAA CCTCATCAAT GCCTGCACCA AGGACATGCC TCCCATGGGC 
TCCCCTGTGA TTCCCACGGA CCTGCCTCTC CGGGCCTCCC CAAGCCACAT CCCCAGCGTG 
GTGGTCCTGC CCATCACACA GCAGGGCTAC CAGCACGAAT ACGTAGATCA GCCCAAAATG 
AGCGAGGTGG TGGCTCAGAT GGCACTGGAG GACCAGGCTG CCACCCTGGA GTATAAGACC 
ATCAAAGAGC ACCTGAGCAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG 
GACAGCCTGC CCCCCAAAGT TCCACAGCGT GAGGCCTCTC TGGGTCCCAC GGGAGCCTCA 
CTGTCCCAGA CCGGCCTGAG CAAGCGGCTG GAAATGCAAC ACTCCTCCTC CTACGGGCTT 
GAATATAAGA GGAGCTACCC CACGAACTCG CTCACAAGAA GCCATCAGGC CACCACTCTC 
AAAAGAAACA ATACTAACTC CTCCAATTCC TCCCACCTCT CCAGGAACCA GAGCCTTGGC 
CGGGGAGACA ACCCACCTCC CGCCCCGCAG AGGGTGGACT CTATCCAGGT GCACAGCCCC 
CAGCCCTCTG GCCAGGCCGT GACTGTTTCG AGGCAGCCCA GCCTCAATGC CTACAACTCA 
CTGACGAGGT CGGGGCTGAA GCGCACCCCC TCGCTAAAGC CAGATGTACC CCCCAAACCT 
TCCTTTGCCC CCCTTTCCAC ATCCATGAAG CCCAATGATG CGTGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( NM_001108430.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101900.2 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant 1, mRNA.

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CloneID	ORa20883
Clone ID Related Accession (Same CDS sequence)	NM_001108430.2 , XM_006222572.3 , XM_006254703.3
Accession Version	NM_001108430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1566921600000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07031972.1, AABR07031971.1 and CK839311.1. On or before Aug 29, 2019 this sequence version replaced XM_006254703.3, XM_006222572.3, NM_001108430.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. The extent of this transcript is supported by orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG GCGTGCCAGT 
TCCCTCTATC CCAGCACCAC TACATCAGAC TCAGCGTCCC GAGACGGGCA TGAGTCTCAG 
GGAGGCATGC TGGACTGGAA TGATCTGCTC AAGGCACCTG GCAACACAGA CCCTTTGGGG 
GCAGTGTCCT CTCATAACCA CCAGGACAAG AAGGGAGTGA TTCGGGAAAG TTACCTCAAA 
AGCAACGACC AGCTCGTTCC TGTCACCCTT TTGGCCATTG CAGTCATTCT GGCTTTTGTC 
ATGGGGGCAG TCTTCTCCGG CATCATCGTG TATTGTGTGT GTGATCACCG GCGCAAAGAC 
GTGGCGGTAG TGCAACGCAA GGAGAAAGAG CTCACCCACT CGCGTCGGGG ATCTATGAGC 
AGTGTCACCA AGCTCAGTGG CCTCTTTGGG GACAGTCAGT CCAAGGACCC AAAGCCTGAG 
GCCATCCTCA CACCACTCAT GCACAATGGC AAACTGGCCA CGCCTAGCAA CACCGCCAAG 
ATGCTCATCA AGGCTGACCA GCATCACTTA GACCTCACCG CCCTGCCCAC CCCGGAGTCC 
ACCCCAACAC TGCAGCAGAA GCGGAAACCC AACCGGGGCA GTCGCGAGTG GGAGAGGAAC 
CAGAACCTCA TCAATGCCTG CACCAAGGAC ATGCCTCCCA TGGGCTCCCC TGTGATTCCC 
ACGGACCTGC CTCTCCGGGC CTCCCCAAGC CACATCCCCA GCGTGGTGGT CCTGCCCATC 
ACACAGCAGG GCTACCAGCA CGAATACGTA GATCAGCCCA AAATGAGCGA GGTGGTGGCT 
CAGATGGCAC TGGAGGACCA GGCTGCCACC CTGGAGTATA AGACCATCAA AGAGCACCTG 
AGCAGCAAGA GTCCCAACCA TGGGGTGAAC CTTGTGGAGA ACCTGGACAG CCTGCCCCCC 
AAAGTTCCAC AGCGTGAGGC CTCTCTGGGT CCCACGGGAG CCTCACTGTC CCAGACCGGC 
CTGAGCAAGC GGCTGGAAAT GCAACACTCC TCCTCCTACG GGCTTGAATA TAAGAGGAGC 
TACCCCACGA ACTCGCTCAC AAGAAGCCAT CAGGCCACCA CTCTCAAAAG AAACAATACT 
AACTCCTCCA ATTCCTCCCA CCTCTCCAGG AACCAGAGCC TTGGCCGGGG AGACAACCCA 
CCTCCCGCCC CGCAGAGGGT GGACTCTATC CAGGTGCACA GCCCCCAGCC CTCTGGCCAG 
GCCGTGACTG TTTCGAGGCA GCCCAGCCTC AATGCCTACA ACTCACTGAC GAGGTCGGGG 
CTGAAGCGCA CCCCCTCGCT AAAGCCAGAT GTACCCCCCA AACCTTCCTT TGCCCCCCTT 
TCCACATCCA TGAAGCCCAA TGATGCGTGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101900.2
CDS	970..4065
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNGRASSLYPSTTTSDSASRDGHESQGGMLDWNDLLKAPGNTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	NM_001108430.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108430.2

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG GCGTGCCAGT 
TCCCTCTATC CCAGCACCAC TACATCAGAC TCAGCGTCCC GAGACGGGCA TGAGTCTCAG 
GGAGGCATGC TGGACTGGAA TGATCTGCTC AAGGCACCTG GCAACACAGA CCCTTTGGGG 
GCAGTGTCCT CTCATAACCA CCAGGACAAG AAGGGAGTGA TTCGGGAAAG TTACCTCAAA 
AGCAACGACC AGCTCGTTCC TGTCACCCTT TTGGCCATTG CAGTCATTCT GGCTTTTGTC 
ATGGGGGCAG TCTTCTCCGG CATCATCGTG TATTGTGTGT GTGATCACCG GCGCAAAGAC 
GTGGCGGTAG TGCAACGCAA GGAGAAAGAG CTCACCCACT CGCGTCGGGG ATCTATGAGC 
AGTGTCACCA AGCTCAGTGG CCTCTTTGGG GACAGTCAGT CCAAGGACCC AAAGCCTGAG 
GCCATCCTCA CACCACTCAT GCACAATGGC AAACTGGCCA CGCCTAGCAA CACCGCCAAG 
ATGCTCATCA AGGCTGACCA GCATCACTTA GACCTCACCG CCCTGCCCAC CCCGGAGTCC 
ACCCCAACAC TGCAGCAGAA GCGGAAACCC AACCGGGGCA GTCGCGAGTG GGAGAGGAAC 
CAGAACCTCA TCAATGCCTG CACCAAGGAC ATGCCTCCCA TGGGCTCCCC TGTGATTCCC 
ACGGACCTGC CTCTCCGGGC CTCCCCAAGC CACATCCCCA GCGTGGTGGT CCTGCCCATC 
ACACAGCAGG GCTACCAGCA CGAATACGTA GATCAGCCCA AAATGAGCGA GGTGGTGGCT 
CAGATGGCAC TGGAGGACCA GGCTGCCACC CTGGAGTATA AGACCATCAA AGAGCACCTG 
AGCAGCAAGA GTCCCAACCA TGGGGTGAAC CTTGTGGAGA ACCTGGACAG CCTGCCCCCC 
AAAGTTCCAC AGCGTGAGGC CTCTCTGGGT CCCACGGGAG CCTCACTGTC CCAGACCGGC 
CTGAGCAAGC GGCTGGAAAT GCAACACTCC TCCTCCTACG GGCTTGAATA TAAGAGGAGC 
TACCCCACGA ACTCGCTCAC AAGAAGCCAT CAGGCCACCA CTCTCAAAAG AAACAATACT 
AACTCCTCCA ATTCCTCCCA CCTCTCCAGG AACCAGAGCC TTGGCCGGGG AGACAACCCA 
CCTCCCGCCC CGCAGAGGGT GGACTCTATC CAGGTGCACA GCCCCCAGCC CTCTGGCCAG 
GCCGTGACTG TTTCGAGGCA GCCCAGCCTC AATGCCTACA ACTCACTGAC GAGGTCGGGG 
CTGAAGCGCA CCCCCTCGCT AAAGCCAGAT GTACCCCCCA AACCTTCCTT TGCCCCCCTT 
TCCACATCCA TGAAGCCCAA TGATGCGTGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_008772186.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770408.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa20882
Clone ID Related Accession (Same CDS sequence)	XM_008772186.1 , XM_003751782.4 , XM_008774127.2 , XM_003753046.4
Accession Version	XM_008772186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC ACAGTGTATG GGCGTGCCAG TTCCCTCTAT 
CCCAGCACCA CTACATCAGA CTCAGCGTCC CGAGACGGGC ATGAGTCTCA GGGAGGCATG 
CTGGACTGGA ATGATCTGCT CAAGGCACCT GGCAACACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTCGTTC CTGTCACCCT TTTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCA 
GTCTTCTCCG GCATCATCGT GTATTGTGTG TGTGATCACC GGCGCAAAGA CGTGGCGGTA 
GTGCAACGCA AGGAGAAAGA GCTCACCCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACAGTCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAATGG CAAACTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACTT AGACCTCACC GCCCTGCCCA CCCCGGAGTC CACCCCAACA 
CTGCAGCAGA AGCGGAAACC CAACCGGGGC AGTCGCGAGT GGGAGAGGAA CCAGAACCTC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGCTCCC CTGTGATTCC CACGGACCTG 
CCTCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACACAGCAG 
GGCTACCAGC ACGAATACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGCAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC CAAAGTTCCA 
CAGCGTGAGG CCTCTCTGGG TCCCACGGGA GCCTCACTGT CCCAGACCGG CCTGAGCAAG 
CGGCTGGAAA TGCAACACTC CTCCTCCTAC GGGCTTGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA TCAGGCCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC CTTGGCCGGG GAGACAACCC ACCTCCCGCC 
CCGCAGAGGG TGGACTCTAT CCAGGTGCAC AGCCCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCCCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCGTG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770408.1
CDS	1776..4922
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNDISTPLPDYEMSFNTVYGRASSLYPSTTTSDSASRDGHESQGGMLDWNDLLKAPGNTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_008772186.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772186.1

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC ACAGTGTATG GGCGTGCCAG TTCCCTCTAT 
CCCAGCACCA CTACATCAGA CTCAGCGTCC CGAGACGGGC ATGAGTCTCA GGGAGGCATG 
CTGGACTGGA ATGATCTGCT CAAGGCACCT GGCAACACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTCGTTC CTGTCACCCT TTTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCA 
GTCTTCTCCG GCATCATCGT GTATTGTGTG TGTGATCACC GGCGCAAAGA CGTGGCGGTA 
GTGCAACGCA AGGAGAAAGA GCTCACCCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACAGTCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAATGG CAAACTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACTT AGACCTCACC GCCCTGCCCA CCCCGGAGTC CACCCCAACA 
CTGCAGCAGA AGCGGAAACC CAACCGGGGC AGTCGCGAGT GGGAGAGGAA CCAGAACCTC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGCTCCC CTGTGATTCC CACGGACCTG 
CCTCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACACAGCAG 
GGCTACCAGC ACGAATACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGCAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC CAAAGTTCCA 
CAGCGTGAGG CCTCTCTGGG TCCCACGGGA GCCTCACTGT CCCAGACCGG CCTGAGCAAG 
CGGCTGGAAA TGCAACACTC CTCCTCCTAC GGGCTTGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA TCAGGCCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC CTTGGCCGGG GAGACAACCC ACCTCCCGCC 
CCGCAGAGGG TGGACTCTAT CCAGGTGCAC AGCCCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCCCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCGTG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_003751782.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003751830.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa20882
Clone ID Related Accession (Same CDS sequence)	XM_008772186.1 , XM_003751782.4 , XM_008774127.2 , XM_003753046.4
Accession Version	XM_003751782.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003751782.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC ACAGTGTATG GGCGTGCCAG TTCCCTCTAT 
CCCAGCACCA CTACATCAGA CTCAGCGTCC CGAGACGGGC ATGAGTCTCA GGGAGGCATG 
CTGGACTGGA ATGATCTGCT CAAGGCACCT GGCAACACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTCGTTC CTGTCACCCT TTTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCA 
GTCTTCTCCG GCATCATCGT GTATTGTGTG TGTGATCACC GGCGCAAAGA CGTGGCGGTA 
GTGCAACGCA AGGAGAAAGA GCTCACCCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACAGTCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAATGG CAAACTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACTT AGACCTCACC GCCCTGCCCA CCCCGGAGTC CACCCCAACA 
CTGCAGCAGA AGCGGAAACC CAACCGGGGC AGTCGCGAGT GGGAGAGGAA CCAGAACCTC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGCTCCC CTGTGATTCC CACGGACCTG 
CCTCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACACAGCAG 
GGCTACCAGC ACGAATACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGCAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC CAAAGTTCCA 
CAGCGTGAGG CCTCTCTGGG TCCCACGGGA GCCTCACTGT CCCAGACCGG CCTGAGCAAG 
CGGCTGGAAA TGCAACACTC CTCCTCCTAC GGGCTTGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA TCAGGCCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC CTTGGCCGGG GAGACAACCC ACCTCCCGCC 
CCGCAGAGGG TGGACTCTAT CCAGGTGCAC AGCCCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCCCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCGTG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003751830.1
CDS	973..4119
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNDISTPLPDYEMSFNTVYGRASSLYPSTTTSDSASRDGHESQGGMLDWNDLLKAPGNTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_003751782.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003751782.4

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC ACAGTGTATG GGCGTGCCAG TTCCCTCTAT 
CCCAGCACCA CTACATCAGA CTCAGCGTCC CGAGACGGGC ATGAGTCTCA GGGAGGCATG 
CTGGACTGGA ATGATCTGCT CAAGGCACCT GGCAACACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTCGTTC CTGTCACCCT TTTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCA 
GTCTTCTCCG GCATCATCGT GTATTGTGTG TGTGATCACC GGCGCAAAGA CGTGGCGGTA 
GTGCAACGCA AGGAGAAAGA GCTCACCCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACAGTCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAATGG CAAACTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACTT AGACCTCACC GCCCTGCCCA CCCCGGAGTC CACCCCAACA 
CTGCAGCAGA AGCGGAAACC CAACCGGGGC AGTCGCGAGT GGGAGAGGAA CCAGAACCTC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGCTCCC CTGTGATTCC CACGGACCTG 
CCTCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACACAGCAG 
GGCTACCAGC ACGAATACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGCAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC CAAAGTTCCA 
CAGCGTGAGG CCTCTCTGGG TCCCACGGGA GCCTCACTGT CCCAGACCGG CCTGAGCAAG 
CGGCTGGAAA TGCAACACTC CTCCTCCTAC GGGCTTGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA TCAGGCCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC CTTGGCCGGG GAGACAACCC ACCTCCCGCC 
CCGCAGAGGG TGGACTCTAT CCAGGTGCAC AGCCCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCCCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCGTG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_008774127.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008772349.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa20882
Clone ID Related Accession (Same CDS sequence)	XM_008772186.1 , XM_003751782.4 , XM_008774127.2 , XM_003753046.4
Accession Version	XM_008774127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008774127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC ACAGTGTATG GGCGTGCCAG TTCCCTCTAT 
CCCAGCACCA CTACATCAGA CTCAGCGTCC CGAGACGGGC ATGAGTCTCA GGGAGGCATG 
CTGGACTGGA ATGATCTGCT CAAGGCACCT GGCAACACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTCGTTC CTGTCACCCT TTTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCA 
GTCTTCTCCG GCATCATCGT GTATTGTGTG TGTGATCACC GGCGCAAAGA CGTGGCGGTA 
GTGCAACGCA AGGAGAAAGA GCTCACCCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACAGTCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAATGG CAAACTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACTT AGACCTCACC GCCCTGCCCA CCCCGGAGTC CACCCCAACA 
CTGCAGCAGA AGCGGAAACC CAACCGGGGC AGTCGCGAGT GGGAGAGGAA CCAGAACCTC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGCTCCC CTGTGATTCC CACGGACCTG 
CCTCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACACAGCAG 
GGCTACCAGC ACGAATACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGCAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC CAAAGTTCCA 
CAGCGTGAGG CCTCTCTGGG TCCCACGGGA GCCTCACTGT CCCAGACCGG CCTGAGCAAG 
CGGCTGGAAA TGCAACACTC CTCCTCCTAC GGGCTTGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA TCAGGCCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC CTTGGCCGGG GAGACAACCC ACCTCCCGCC 
CCGCAGAGGG TGGACTCTAT CCAGGTGCAC AGCCCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCCCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCGTG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008772349.1
CDS	2618..5764
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNDISTPLPDYEMSFNTVYGRASSLYPSTTTSDSASRDGHESQGGMLDWNDLLKAPGNTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_008774127.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008774127.2

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC ACAGTGTATG GGCGTGCCAG TTCCCTCTAT 
CCCAGCACCA CTACATCAGA CTCAGCGTCC CGAGACGGGC ATGAGTCTCA GGGAGGCATG 
CTGGACTGGA ATGATCTGCT CAAGGCACCT GGCAACACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTCGTTC CTGTCACCCT TTTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCA 
GTCTTCTCCG GCATCATCGT GTATTGTGTG TGTGATCACC GGCGCAAAGA CGTGGCGGTA 
GTGCAACGCA AGGAGAAAGA GCTCACCCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACAGTCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAATGG CAAACTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACTT AGACCTCACC GCCCTGCCCA CCCCGGAGTC CACCCCAACA 
CTGCAGCAGA AGCGGAAACC CAACCGGGGC AGTCGCGAGT GGGAGAGGAA CCAGAACCTC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGCTCCC CTGTGATTCC CACGGACCTG 
CCTCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACACAGCAG 
GGCTACCAGC ACGAATACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGCAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC CAAAGTTCCA 
CAGCGTGAGG CCTCTCTGGG TCCCACGGGA GCCTCACTGT CCCAGACCGG CCTGAGCAAG 
CGGCTGGAAA TGCAACACTC CTCCTCCTAC GGGCTTGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA TCAGGCCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC CTTGGCCGGG GAGACAACCC ACCTCCCGCC 
CCGCAGAGGG TGGACTCTAT CCAGGTGCAC AGCCCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCCCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCGTG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_003753046.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003753094.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa20882
Clone ID Related Accession (Same CDS sequence)	XM_008772186.1 , XM_003751782.4 , XM_008774127.2 , XM_003753046.4
Accession Version	XM_003753046.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003753046.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC ACAGTGTATG GGCGTGCCAG TTCCCTCTAT 
CCCAGCACCA CTACATCAGA CTCAGCGTCC CGAGACGGGC ATGAGTCTCA GGGAGGCATG 
CTGGACTGGA ATGATCTGCT CAAGGCACCT GGCAACACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTCGTTC CTGTCACCCT TTTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCA 
GTCTTCTCCG GCATCATCGT GTATTGTGTG TGTGATCACC GGCGCAAAGA CGTGGCGGTA 
GTGCAACGCA AGGAGAAAGA GCTCACCCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACAGTCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAATGG CAAACTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACTT AGACCTCACC GCCCTGCCCA CCCCGGAGTC CACCCCAACA 
CTGCAGCAGA AGCGGAAACC CAACCGGGGC AGTCGCGAGT GGGAGAGGAA CCAGAACCTC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGCTCCC CTGTGATTCC CACGGACCTG 
CCTCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACACAGCAG 
GGCTACCAGC ACGAATACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGCAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC CAAAGTTCCA 
CAGCGTGAGG CCTCTCTGGG TCCCACGGGA GCCTCACTGT CCCAGACCGG CCTGAGCAAG 
CGGCTGGAAA TGCAACACTC CTCCTCCTAC GGGCTTGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA TCAGGCCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC CTTGGCCGGG GAGACAACCC ACCTCCCGCC 
CCGCAGAGGG TGGACTCTAT CCAGGTGCAC AGCCCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCCCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCGTG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003753094.1
CDS	973..4119
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNDISTPLPDYEMSFNTVYGRASSLYPSTTTSDSASRDGHESQGGMLDWNDLLKAPGNTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_003753046.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003753046.4

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGA CATTTCAACT 
CCTCTACCAG ATTATGAAAT GTCTTTCAAC ACAGTGTATG GGCGTGCCAG TTCCCTCTAT 
CCCAGCACCA CTACATCAGA CTCAGCGTCC CGAGACGGGC ATGAGTCTCA GGGAGGCATG 
CTGGACTGGA ATGATCTGCT CAAGGCACCT GGCAACACAG ACCCTTTGGG GGCAGTGTCC 
TCTCATAACC ACCAGGACAA GAAGGGAGTG ATTCGGGAAA GTTACCTCAA AAGCAACGAC 
CAGCTCGTTC CTGTCACCCT TTTGGCCATT GCAGTCATTC TGGCTTTTGT CATGGGGGCA 
GTCTTCTCCG GCATCATCGT GTATTGTGTG TGTGATCACC GGCGCAAAGA CGTGGCGGTA 
GTGCAACGCA AGGAGAAAGA GCTCACCCAC TCGCGTCGGG GATCTATGAG CAGTGTCACC 
AAGCTCAGTG GCCTCTTTGG GGACAGTCAG TCCAAGGACC CAAAGCCTGA GGCCATCCTC 
ACACCACTCA TGCACAATGG CAAACTGGCC ACGCCTAGCA ACACCGCCAA GATGCTCATC 
AAGGCTGACC AGCATCACTT AGACCTCACC GCCCTGCCCA CCCCGGAGTC CACCCCAACA 
CTGCAGCAGA AGCGGAAACC CAACCGGGGC AGTCGCGAGT GGGAGAGGAA CCAGAACCTC 
ATCAATGCCT GCACCAAGGA CATGCCTCCC ATGGGCTCCC CTGTGATTCC CACGGACCTG 
CCTCTCCGGG CCTCCCCAAG CCACATCCCC AGCGTGGTGG TCCTGCCCAT CACACAGCAG 
GGCTACCAGC ACGAATACGT AGATCAGCCC AAAATGAGCG AGGTGGTGGC TCAGATGGCA 
CTGGAGGACC AGGCTGCCAC CCTGGAGTAT AAGACCATCA AAGAGCACCT GAGCAGCAAG 
AGTCCCAACC ATGGGGTGAA CCTTGTGGAG AACCTGGACA GCCTGCCCCC CAAAGTTCCA 
CAGCGTGAGG CCTCTCTGGG TCCCACGGGA GCCTCACTGT CCCAGACCGG CCTGAGCAAG 
CGGCTGGAAA TGCAACACTC CTCCTCCTAC GGGCTTGAAT ATAAGAGGAG CTACCCCACG 
AACTCGCTCA CAAGAAGCCA TCAGGCCACC ACTCTCAAAA GAAACAATAC TAACTCCTCC 
AATTCCTCCC ACCTCTCCAG GAACCAGAGC CTTGGCCGGG GAGACAACCC ACCTCCCGCC 
CCGCAGAGGG TGGACTCTAT CCAGGTGCAC AGCCCCCAGC CCTCTGGCCA GGCCGTGACT 
GTTTCGAGGC AGCCCAGCCT CAATGCCTAC AACTCACTGA CGAGGTCGGG GCTGAAGCGC 
ACCCCCTCGC TAAAGCCAGA TGTACCCCCC AAACCTTCCT TTGCCCCCCT TTCCACATCC 
ATGAAGCCCA ATGATGCGTG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_006222572.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006222634.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X3, mRNA.

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CloneID	ORa20883
Clone ID Related Accession (Same CDS sequence)	NM_001108430.2 , XM_006222572.3 , XM_006254703.3
Accession Version	XM_006222572.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006222572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG GCGTGCCAGT 
TCCCTCTATC CCAGCACCAC TACATCAGAC TCAGCGTCCC GAGACGGGCA TGAGTCTCAG 
GGAGGCATGC TGGACTGGAA TGATCTGCTC AAGGCACCTG GCAACACAGA CCCTTTGGGG 
GCAGTGTCCT CTCATAACCA CCAGGACAAG AAGGGAGTGA TTCGGGAAAG TTACCTCAAA 
AGCAACGACC AGCTCGTTCC TGTCACCCTT TTGGCCATTG CAGTCATTCT GGCTTTTGTC 
ATGGGGGCAG TCTTCTCCGG CATCATCGTG TATTGTGTGT GTGATCACCG GCGCAAAGAC 
GTGGCGGTAG TGCAACGCAA GGAGAAAGAG CTCACCCACT CGCGTCGGGG ATCTATGAGC 
AGTGTCACCA AGCTCAGTGG CCTCTTTGGG GACAGTCAGT CCAAGGACCC AAAGCCTGAG 
GCCATCCTCA CACCACTCAT GCACAATGGC AAACTGGCCA CGCCTAGCAA CACCGCCAAG 
ATGCTCATCA AGGCTGACCA GCATCACTTA GACCTCACCG CCCTGCCCAC CCCGGAGTCC 
ACCCCAACAC TGCAGCAGAA GCGGAAACCC AACCGGGGCA GTCGCGAGTG GGAGAGGAAC 
CAGAACCTCA TCAATGCCTG CACCAAGGAC ATGCCTCCCA TGGGCTCCCC TGTGATTCCC 
ACGGACCTGC CTCTCCGGGC CTCCCCAAGC CACATCCCCA GCGTGGTGGT CCTGCCCATC 
ACACAGCAGG GCTACCAGCA CGAATACGTA GATCAGCCCA AAATGAGCGA GGTGGTGGCT 
CAGATGGCAC TGGAGGACCA GGCTGCCACC CTGGAGTATA AGACCATCAA AGAGCACCTG 
AGCAGCAAGA GTCCCAACCA TGGGGTGAAC CTTGTGGAGA ACCTGGACAG CCTGCCCCCC 
AAAGTTCCAC AGCGTGAGGC CTCTCTGGGT CCCACGGGAG CCTCACTGTC CCAGACCGGC 
CTGAGCAAGC GGCTGGAAAT GCAACACTCC TCCTCCTACG GGCTTGAATA TAAGAGGAGC 
TACCCCACGA ACTCGCTCAC AAGAAGCCAT CAGGCCACCA CTCTCAAAAG AAACAATACT 
AACTCCTCCA ATTCCTCCCA CCTCTCCAGG AACCAGAGCC TTGGCCGGGG AGACAACCCA 
CCTCCCGCCC CGCAGAGGGT GGACTCTATC CAGGTGCACA GCCCCCAGCC CTCTGGCCAG 
GCCGTGACTG TTTCGAGGCA GCCCAGCCTC AATGCCTACA ACTCACTGAC GAGGTCGGGG 
CTGAAGCGCA CCCCCTCGCT AAAGCCAGAT GTACCCCCCA AACCTTCCTT TGCCCCCCTT 
TCCACATCCA TGAAGCCCAA TGATGCGTGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006222634.1
CDS	970..4065
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNGRASSLYPSTTTSDSASRDGHESQGGMLDWNDLLKAPGNTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_006222572.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006222572.3

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG GCGTGCCAGT 
TCCCTCTATC CCAGCACCAC TACATCAGAC TCAGCGTCCC GAGACGGGCA TGAGTCTCAG 
GGAGGCATGC TGGACTGGAA TGATCTGCTC AAGGCACCTG GCAACACAGA CCCTTTGGGG 
GCAGTGTCCT CTCATAACCA CCAGGACAAG AAGGGAGTGA TTCGGGAAAG TTACCTCAAA 
AGCAACGACC AGCTCGTTCC TGTCACCCTT TTGGCCATTG CAGTCATTCT GGCTTTTGTC 
ATGGGGGCAG TCTTCTCCGG CATCATCGTG TATTGTGTGT GTGATCACCG GCGCAAAGAC 
GTGGCGGTAG TGCAACGCAA GGAGAAAGAG CTCACCCACT CGCGTCGGGG ATCTATGAGC 
AGTGTCACCA AGCTCAGTGG CCTCTTTGGG GACAGTCAGT CCAAGGACCC AAAGCCTGAG 
GCCATCCTCA CACCACTCAT GCACAATGGC AAACTGGCCA CGCCTAGCAA CACCGCCAAG 
ATGCTCATCA AGGCTGACCA GCATCACTTA GACCTCACCG CCCTGCCCAC CCCGGAGTCC 
ACCCCAACAC TGCAGCAGAA GCGGAAACCC AACCGGGGCA GTCGCGAGTG GGAGAGGAAC 
CAGAACCTCA TCAATGCCTG CACCAAGGAC ATGCCTCCCA TGGGCTCCCC TGTGATTCCC 
ACGGACCTGC CTCTCCGGGC CTCCCCAAGC CACATCCCCA GCGTGGTGGT CCTGCCCATC 
ACACAGCAGG GCTACCAGCA CGAATACGTA GATCAGCCCA AAATGAGCGA GGTGGTGGCT 
CAGATGGCAC TGGAGGACCA GGCTGCCACC CTGGAGTATA AGACCATCAA AGAGCACCTG 
AGCAGCAAGA GTCCCAACCA TGGGGTGAAC CTTGTGGAGA ACCTGGACAG CCTGCCCCCC 
AAAGTTCCAC AGCGTGAGGC CTCTCTGGGT CCCACGGGAG CCTCACTGTC CCAGACCGGC 
CTGAGCAAGC GGCTGGAAAT GCAACACTCC TCCTCCTACG GGCTTGAATA TAAGAGGAGC 
TACCCCACGA ACTCGCTCAC AAGAAGCCAT CAGGCCACCA CTCTCAAAAG AAACAATACT 
AACTCCTCCA ATTCCTCCCA CCTCTCCAGG AACCAGAGCC TTGGCCGGGG AGACAACCCA 
CCTCCCGCCC CGCAGAGGGT GGACTCTATC CAGGTGCACA GCCCCCAGCC CTCTGGCCAG 
GCCGTGACTG TTTCGAGGCA GCCCAGCCTC AATGCCTACA ACTCACTGAC GAGGTCGGGG 
CTGAAGCGCA CCCCCTCGCT AAAGCCAGAT GTACCCCCCA AACCTTCCTT TGCCCCCCTT 
TCCACATCCA TGAAGCCCAA TGATGCGTGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema6a ( XM_006254703.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254765.1 Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X3, mRNA.

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CloneID	ORa20883
Clone ID Related Accession (Same CDS sequence)	NM_001108430.2 , XM_006222572.3 , XM_006254703.3
Accession Version	XM_006254703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG GCGTGCCAGT 
TCCCTCTATC CCAGCACCAC TACATCAGAC TCAGCGTCCC GAGACGGGCA TGAGTCTCAG 
GGAGGCATGC TGGACTGGAA TGATCTGCTC AAGGCACCTG GCAACACAGA CCCTTTGGGG 
GCAGTGTCCT CTCATAACCA CCAGGACAAG AAGGGAGTGA TTCGGGAAAG TTACCTCAAA 
AGCAACGACC AGCTCGTTCC TGTCACCCTT TTGGCCATTG CAGTCATTCT GGCTTTTGTC 
ATGGGGGCAG TCTTCTCCGG CATCATCGTG TATTGTGTGT GTGATCACCG GCGCAAAGAC 
GTGGCGGTAG TGCAACGCAA GGAGAAAGAG CTCACCCACT CGCGTCGGGG ATCTATGAGC 
AGTGTCACCA AGCTCAGTGG CCTCTTTGGG GACAGTCAGT CCAAGGACCC AAAGCCTGAG 
GCCATCCTCA CACCACTCAT GCACAATGGC AAACTGGCCA CGCCTAGCAA CACCGCCAAG 
ATGCTCATCA AGGCTGACCA GCATCACTTA GACCTCACCG CCCTGCCCAC CCCGGAGTCC 
ACCCCAACAC TGCAGCAGAA GCGGAAACCC AACCGGGGCA GTCGCGAGTG GGAGAGGAAC 
CAGAACCTCA TCAATGCCTG CACCAAGGAC ATGCCTCCCA TGGGCTCCCC TGTGATTCCC 
ACGGACCTGC CTCTCCGGGC CTCCCCAAGC CACATCCCCA GCGTGGTGGT CCTGCCCATC 
ACACAGCAGG GCTACCAGCA CGAATACGTA GATCAGCCCA AAATGAGCGA GGTGGTGGCT 
CAGATGGCAC TGGAGGACCA GGCTGCCACC CTGGAGTATA AGACCATCAA AGAGCACCTG 
AGCAGCAAGA GTCCCAACCA TGGGGTGAAC CTTGTGGAGA ACCTGGACAG CCTGCCCCCC 
AAAGTTCCAC AGCGTGAGGC CTCTCTGGGT CCCACGGGAG CCTCACTGTC CCAGACCGGC 
CTGAGCAAGC GGCTGGAAAT GCAACACTCC TCCTCCTACG GGCTTGAATA TAAGAGGAGC 
TACCCCACGA ACTCGCTCAC AAGAAGCCAT CAGGCCACCA CTCTCAAAAG AAACAATACT 
AACTCCTCCA ATTCCTCCCA CCTCTCCAGG AACCAGAGCC TTGGCCGGGG AGACAACCCA 
CCTCCCGCCC CGCAGAGGGT GGACTCTATC CAGGTGCACA GCCCCCAGCC CTCTGGCCAG 
GCCGTGACTG TTTCGAGGCA GCCCAGCCTC AATGCCTACA ACTCACTGAC GAGGTCGGGG 
CTGAAGCGCA CCCCCTCGCT AAAGCCAGAT GTACCCCCCA AACCTTCCTT TGCCCCCCTT 
TCCACATCCA TGAAGCCCAA TGATGCGTGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254765.1
CDS	970..4065
Translation	MRPAALLLCLTLLRCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYIAARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAFNPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKARLNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPDSTWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRLTKIAVDNTAGPYENHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGMQLDKASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVKETGSCAHLSPLSRLAFEQDIERGNTDGLGDCHNSFVALNGRASSLYPSTTTSDSASRDGHESQGGMLDWNDLLKAPGNTDPLGAVSSHNHQDKKGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMSSVTKLSGLFGDSQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKPNRGSREWERNQNLINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVAQMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPTGASLSQTGLSKRLEMQHSSSYGLEYKRSYPTNSLTRSHQATTLKRNNTNSSNSSHLSRNQSLGRGDNPPPAPQRVDSIQVHSPQPSGQAVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT

Target ORF information:

RefSeq Version	XM_006254703.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 6A (Sema6a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254703.3

ORF Insert Sequence:

ATGAGGCCAG CAGCCTTACT GCTGTGTCTC ACACTGCTAC GCTGCGCTGG GGCTGGTTTC 
CCAGAAGATT CTGAGCCGAT CAGTATTTCG CATGGCAACT ATACAAAACA GTATCCGGTG 
TTTGTGGGCC ACAAGCCAGG ACGGAACACC ACGCAGAGGC ACAGGCTGGA CATCCAGATG 
ATCATGATCA TGAACAGAAC CCTCTACATT GCTGCTCGAG ACCATATTTA CACTGTTGAT 
ATAGACACAT CCCACACAGA AGAAATTTAC TGTAGCAAAA AACTGACATG GAAATCTAGA 
CAGGCTGATG TAGACACATG CAGGATGAAG GGGAAGCATA AGGATGAATG TCACAACTTC 
ATTAAAGTTC TTCTCAAGAA GAACGATGAC ACGCTGTTTG TCTGTGGAAC CAATGCCTTC 
AACCCTTCCT GCAGAAACTA CAGGGTGGAT ACTTTGGAAA CTTTTGGGGA TGAATTTAGT 
GGAATGGCCA GATGCCCTTA TGATGCCAAA CATGCCAACA TCGCTCTGTT TGCAGATGGG 
AAACTGTACT CGGCCACAGT GACTGACTTT CTGGCCATTG ATGCGGTCAT TTACAGGAGC 
CTTGGAGACA GTCCTACCCT CAGGACTGTC AAGCATGATT CAAAGTGGTT GAAAGAGCCG 
TACTTTGTCC AAGCAGTGGA TTACGGAGAC TATATCTACT TCTTCTTCAG AGAAATTGCA 
GTAGAGTACA ACACTATGGG GAAGGTTGTA TTCCCCAGGG TGGCTCAGGT TTGTAAGAAC 
GACATGGGAG GGTCTCAGAG AGTCCTGGAG AAGCAGTGGA CATCATTCCT GAAGGCTCGC 
CTGAACTGCT CCGTGCCTGG AGACTCTCAT TTTTATTTCA ATATACTCCA GGCAGTTACA 
GATGTGATTC GAATTAATGG CCGTGATGTT GTCTTGGCAA CCTTTTCCAC ACCTTATAAC 
AGCATCCCTG GTTCTGCAGT CTGTGCCTAT GACATGCTTG ACATTGCCAA TGTTTTTACT 
GGGAGATTCA AGGAACAGAA ATCACCTGAT TCTACCTGGA CACCGGTTCC AGATGAGCGA 
GTCCCTAAGC CCAGGCCAGG CTGTTGTGCT GGATCATCCT CTTTAGAAAA ATATGCAACC 
TCCAATGAGT TTCCTGATGA TACCCTGAAC TTCATAAAGA CGCATCCACT CATGGACGAG 
GCAGTGCCTT CCATTATCAA CAGACCTTGG TTCCTGAGAA CAATGGTCAG ATACCGCCTG 
ACCAAAATTG CAGTAGACAA CACTGCTGGG CCATATGAGA ATCACACTGT GGTTTTCCTG 
GGATCAGAAA AGGGAATCAT CTTGAAGTTC TTGGCCAGGA TAGGAAGCAG TGGTTTCCTA 
AATGGCAGCC TTTTCCTGGA GGAGATGAAT GTTTACAACC CAGAAAAGTG CAGCTATGAT 
GGTGTAGAAG ACAAAAGGAT CATGGGTATG CAGCTCGACA AAGCAAGTGG TTCACTTTAT 
GTTGCATTCT CTACTTGTGT GATAAAGGTG CCTCTTGGCC GCTGTGAGCG ACATGGGAAG 
TGTAAAAAAA CCTGTATCGC CTCCAGAGAC CCATATTGTG GGTGGGTAAA GGAAACTGGT 
TCCTGTGCCC ATCTGTCACC CCTTAGCAGA CTGGCTTTTG AGCAAGACAT AGAGCGTGGC 
AATACAGACG GCCTAGGAGA CTGTCACAAT TCCTTCGTGG CACTGAATGG GCGTGCCAGT 
TCCCTCTATC CCAGCACCAC TACATCAGAC TCAGCGTCCC GAGACGGGCA TGAGTCTCAG 
GGAGGCATGC TGGACTGGAA TGATCTGCTC AAGGCACCTG GCAACACAGA CCCTTTGGGG 
GCAGTGTCCT CTCATAACCA CCAGGACAAG AAGGGAGTGA TTCGGGAAAG TTACCTCAAA 
AGCAACGACC AGCTCGTTCC TGTCACCCTT TTGGCCATTG CAGTCATTCT GGCTTTTGTC 
ATGGGGGCAG TCTTCTCCGG CATCATCGTG TATTGTGTGT GTGATCACCG GCGCAAAGAC 
GTGGCGGTAG TGCAACGCAA GGAGAAAGAG CTCACCCACT CGCGTCGGGG ATCTATGAGC 
AGTGTCACCA AGCTCAGTGG CCTCTTTGGG GACAGTCAGT CCAAGGACCC AAAGCCTGAG 
GCCATCCTCA CACCACTCAT GCACAATGGC AAACTGGCCA CGCCTAGCAA CACCGCCAAG 
ATGCTCATCA AGGCTGACCA GCATCACTTA GACCTCACCG CCCTGCCCAC CCCGGAGTCC 
ACCCCAACAC TGCAGCAGAA GCGGAAACCC AACCGGGGCA GTCGCGAGTG GGAGAGGAAC 
CAGAACCTCA TCAATGCCTG CACCAAGGAC ATGCCTCCCA TGGGCTCCCC TGTGATTCCC 
ACGGACCTGC CTCTCCGGGC CTCCCCAAGC CACATCCCCA GCGTGGTGGT CCTGCCCATC 
ACACAGCAGG GCTACCAGCA CGAATACGTA GATCAGCCCA AAATGAGCGA GGTGGTGGCT 
CAGATGGCAC TGGAGGACCA GGCTGCCACC CTGGAGTATA AGACCATCAA AGAGCACCTG 
AGCAGCAAGA GTCCCAACCA TGGGGTGAAC CTTGTGGAGA ACCTGGACAG CCTGCCCCCC 
AAAGTTCCAC AGCGTGAGGC CTCTCTGGGT CCCACGGGAG CCTCACTGTC CCAGACCGGC 
CTGAGCAAGC GGCTGGAAAT GCAACACTCC TCCTCCTACG GGCTTGAATA TAAGAGGAGC 
TACCCCACGA ACTCGCTCAC AAGAAGCCAT CAGGCCACCA CTCTCAAAAG AAACAATACT 
AACTCCTCCA ATTCCTCCCA CCTCTCCAGG AACCAGAGCC TTGGCCGGGG AGACAACCCA 
CCTCCCGCCC CGCAGAGGGT GGACTCTATC CAGGTGCACA GCCCCCAGCC CTCTGGCCAG 
GCCGTGACTG TTTCGAGGCA GCCCAGCCTC AATGCCTACA ACTCACTGAC GAGGTCGGGG 
CTGAAGCGCA CCCCCTCGCT AAAGCCAGAT GTACCCCCCA AACCTTCCTT TGCCCCCCTT 
TCCACATCCA TGAAGCCCAA TGATGCGTGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Sema6a
Entrez Gene ID	361324
Full Name	semaphorin 6A
General protein information	Preferred Names semaphorin 6A Names semaphorin-6A sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A semaphorin 6A-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q11

Homo sapiens (human)	SEMA6A	NP_065847.1
Gallus gallus (chicken)	SEMA6A	XP_003643120.1
Pan troglodytes (chimpanzee)	SEMA6A	XP_001151177.1
Macaca mulatta (Rhesus monkey)	SEMA6A	XP_002804530.1
Canis lupus familiaris (dog)	SEMA6A	XP_858843.1
Mus musculus (house mouse)	Sema6a	NP_061214.2
Xenopus tropicalis (tropical clawed frog)	sema6a	XP_002931763.2
Bos taurus (cattle)	SEMA6A	NP_001180138.1
Danio rerio (zebrafish)	sema6a	NP_956286.1
Rattus norvegicus (Norway rat)	Sema6a	XP_003751830.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Sema6a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

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