sema6a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema6a
Entrez Gene ID	336060
Full Name	sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Synonyms	fj46c12,wu:fj46c12,zgc:66106
General protein information	Preferred Names sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A Names semaphorin-6A semaphorin 6a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199992.1	NP_956286.1	semaphorin-6A precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	SEMA6A	NP_065847.1
Gallus gallus (chicken)	SEMA6A	XP_003643120.1
Pan troglodytes (chimpanzee)	SEMA6A	XP_001151177.1
Macaca mulatta (Rhesus monkey)	SEMA6A	XP_002804530.1
Canis lupus familiaris (dog)	SEMA6A	XP_858843.1
Mus musculus (house mouse)	Sema6a	NP_061214.2
Xenopus tropicalis (tropical clawed frog)	sema6a	XP_002931763.2
Bos taurus (cattle)	SEMA6A	NP_001180138.1
Danio rerio (zebrafish)	sema6a	NP_956286.1
Rattus norvegicus (Norway rat)	Sema6a	XP_003751830.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Emerson SE, St Clair RM, Waldron AL, Bruno SR, Duong A, Driscoll HE, Ballif BA, McFarlane S, Ebert AM
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 07)

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Sema6a and Plxna2 mediate spatially regulated repulsion within the developing eye to promote eye vesicle cohesion.
Ebert AM, Childs SJ, Hehr CL, Cechmanek PB, McFarlane S
Development (Cambridge, England)141(12)2473-82(2014 Jun)

Neuronal expression of class 6 semaphorins in zebrafish.
Ebert AM, Lamont RE, Childs SJ, McFarlane S
Gene expression patterns : GEP12(3-4)117-22(2012 Mar-Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sema6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31810	NM_199992.1 Latest version!	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (sema6a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema6a ( NM_199992.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956286.1 Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (sema6a), mRNA.

Price & Availability↑

CloneID	ODa31810
Clone ID Related Accession (Same CDS sequence)	NM_199992.1
Accession Version	NM_199992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529942400000
Organism	Danio rerio(zebrafish)
Product	semaphorin-6A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061707.1. ##Evidence-Data-START## Transcript exon combination :: BC061707.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCGAGCGC AGGCCCTGCT GCTGTACCTG ACGCTGCTGC AGACGGCTGG AGCCGCTTTC 
CCAGAAGACA CCGAGCCCAT CAGCATCGCA CATGGAAACT ACACTAAACA GTACCCAGTG 
TTCGTGGGAC ACAAACCAGG CAGGAATAAC ACACAGCGAC ACAAACTGGA CATCCAGCTG 
ATCGTCATCA TGAACCGGAC GCTGTATGTG GCTGCGAGGG ATCACATTTA CACGCTGGAC 
ATGGAGACGT CCAGCACAGA GGAGATCTTC TTCAGTAAGA AGCTGACCTG GAAGTCCAGA 
CAGGCGGATG TGGACACGTG CAGGATGAAG GGCAAACACA AGGACGAGTG TCATAACTTC 
ATCAAGGTGC TCCTGCAGCA GAGTGAGGAC TCTCTGTTTG TGTGCGGAAC CAACGCCTTC 
AACCCGTCCT GCAGAACCTA CAGGATGGAC AGTCTGGACG CTCTGGGTGA GGAGATCAGT 
GGGATGGCCC GCTGCCCGTA CGACGCCAAA CACGCCAACG TGGCCCTGTT CGCTGATGGG 
AAGTTATATT CCGCCACAGT CACAGATTTC CTGGCTATCG ACGCAGTGAT CTACCGCAGT 
TTAGGAGACA GTCCCACACT CAGAACCGTC AAACACGACT CCAAATGGCT GAAGGAGCCG 
TATTTCGTGC AGGCGGTGGA TTACGAGGAC TTCATCTACT TCTTCTTTCG GGAAATCGCC 
ATGGAGTACA ACAGCATGGG GAAGGTGGTG TTTCCGCGTG TGGCGCGTGT GTGTAAGAGC 
GACAGCGGCG GCTCTCAGCG TGTGCTGGAG AAACAGTGGA CATCTTTCCT GAAGACGCGC 
CTGAACTGCT CCATTCCTGG AGACTCACAC TTCTACTTCA ACATCCTGCA GGCCGTGACC 
GACGTCATAC ACATCAGCGG ACGAGACGTG GTCATGGCCA CCTTCTCCAC ACCCTACAAC 
AGTATTCCGG GATCAGCTGT GTGTGCGTAT GACATGTCAG AGATCGCTCT GGCCTTCACC 
GGACGCTTCA AAGAGCAGAA ATCACCTGAC TCCACCTGGA CACCTGTGCC CGAGGAGAGG 
GTGCCCAAAC CCAGGCCGGG TGTGTGTGCC GGCTCGTCCT CCGTGGAGAA GTTTAAAGTG 
TCGAGTGAGT TTCCGGACGA TTCTCTGAGC TTCATCAAAG CCCACCCGCT GATGGAGGAG 
GCCGTGCCCG CCATCGCCAA CCGGCCCTGG TTCCTCAAGA CCATGGTGAG GTACCGGCTC 
ACTCGTATCG CAGTGGATAA CGCCGCCGGG CCGCACAGTA ACCATACGGT GGTCTTCCTG 
GGCTCTGAGC GAGGAATCGT CCTGAAGTTT CTGGCTAAGA TCCGCAGCGG CGCCCTGAAC 
GACAGCCTGT TCCTGGAGGA GCTGAACGTC TACAACGCAG AGAAGTGCAG TATTGACGGT 
GTGGAGGATA AGCGCATCAT CAGTATGCAG ATCGACAGCC GCAGCCGATC TCTGTTCGTG 
GCCTTTAGCT CCTGTGTGGT TCGAGTCCCG CTCGCGCGCT GCCAGCGCCA TGGAAAGTGC 
AAGAAGTCCT GTATCGCCTC CAGAGACCCG TACTGCGGCT GGGTGTCCGA GGGCGCCTGC 
AGAGAAATCA CATCTGATGC AAAATGGCCG TTCGAGCAGG ACGTGGAGCA GGGAAACACA 
GATGGACTGG GCGACTGCCA AAATTCGTTC GTGGCTCTGA ACGGCCGTGT CTCCTCCCCC 
TCAGTGTCTC CAGCAGTGGT CAGTGTTCCT CTGGGTCGGG GTCGAGTGCT GAAGGATCAC 
CAGGAGCCGC TGGTGTCCGG GTCCTCAGAG ACGCAGCAGG AGAGCGCGGG AGTGATCCGC 
GAGACCTTCC ACCGGGACCG GGATCAGATG GTTCCAGTGA CGCTGTTGGC CATCGCTGTG 
ATCCTGGCCT TCAGCATGGG CGCCATCTTC AGCGGGATCC TGGTGTACTG TGTGTGTGAC 
CACCACCGCC GGCGGGAGTT CCCCATGTCA GGACGCAAAG AGAAGGACTG TGCGGCGTCC 
CGCCGCGGCT CCATGAACAG TGTGACCAAG CTGACGGGGC TGTTCGAGAC GCAGGCTAAA 
GACGGGCGTG CAGAGCAGAT GCTGACCCCG CTGATGAACG GCCGGCTGGT CAACGGGCGG 
ATGCTGATTA AAGCGGAGCA GCACCTGGAC CTGTCCGCCC TGCCCACACC CGAGTCCACA 
CCACTGCAGC CACGCCGCAA GCCTAGCAGG GGGAGCCGAG AGTGGGAGCG CAACCAGAAC 
CTGATCAACG CCTGCACCAA AGAGCTGTCA TCAATGGGGT CACCTGTGAT CCCGACAGAG 
CTGCCGCTTC GGGCATCCCC GGGCCACATC TCCAGTGTGG TGGTGCTGCC ACTGCCACCA 
CATCAGTCGT ACCAGCACGA GTACGTGGAG CATCCGCAGC AGCGCCCTGA CGATCCAGCG 
GCCACGCTGG AGTTCCAGAC GCTGCGGAGT CCGTGCCGTG GTGCAGAGGA GGAGGTGGTG 
CCGCCACGAG TGCCCCAGCG GGAGGCGTCC CTGGTGCCAC CGGCCGTCCC ACAGATGGGC 
AAACGGCTAG ACATGCACTC CTCCATGTAC GCCCGCGGGT TCCCCATGAG CTCCGGCCTG 
AAGAAGCAGC ACAACACCAA CTCCTCCAAC TCCTCACACA TGTCCAGGAA CCACAGCTTC 
CGTGTGGAGA CGCCTCCGCC CGCCCCGCAG CGCGTGGACT CCATGCAGGT CTCCTCTCCG 
GGCCTCGGCC TCTCCCACCA CCCCAGCCTC AGCTCTTTCG GCTCTCTGTC TCGCTGCGGA 
GCCCGGCAGA TGAAGCCCGA CGTGCCGCCC AAACCCTCGG CCGTGTCCTA CTGCAGCAAA 
GCCACATCCG GAGACTCCTG CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956286.1
CDS	120..3026
Translation	MRAQALLLYLTLLQTAGAAFPEDTEPISIAHGNYTKQYPVFVGHKPGRNNTQRHKLDIQLIVIMNRTLYVAARDHIYTLDMETSSTEEIFFSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLQQSEDSLFVCGTNAFNPSCRTYRMDSLDALGEEISGMARCPYDAKHANVALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYEDFIYFFFREIAMEYNSMGKVVFPRVARVCKSDSGGSQRVLEKQWTSFLKTRLNCSIPGDSHFYFNILQAVTDVIHISGRDVVMATFSTPYNSIPGSAVCAYDMSEIALAFTGRFKEQKSPDSTWTPVPEERVPKPRPGVCAGSSSVEKFKVSSEFPDDSLSFIKAHPLMEEAVPAIANRPWFLKTMVRYRLTRIAVDNAAGPHSNHTVVFLGSERGIVLKFLAKIRSGALNDSLFLEELNVYNAEKCSIDGVEDKRIISMQIDSRSRSLFVAFSSCVVRVPLARCQRHGKCKKSCIASRDPYCGWVSEGACREITSDAKWPFEQDVEQGNTDGLGDCQNSFVALNGRVSSPSVSPAVVSVPLGRGRVLKDHQEPLVSGSSETQQESAGVIRETFHRDRDQMVPVTLLAIAVILAFSMGAIFSGILVYCVCDHHRRREFPMSGRKEKDCAASRRGSMNSVTKLTGLFETQAKDGRAEQMLTPLMNGRLVNGRMLIKAEQHLDLSALPTPESTPLQPRRKPSRGSREWERNQNLINACTKELSSMGSPVIPTELPLRASPGHISSVVVLPLPPHQSYQHEYVEHPQQRPDDPAATLEFQTLRSPCRGAEEEVVPPRVPQREASLVPPAVPQMGKRLDMHSSMYARGFPMSSGLKKQHNTNSSNSSHMSRNHSFRVETPPPAPQRVDSMQVSSPGLGLSHHPSLSSFGSLSRCGARQMKPDVPPKPSAVSYCSKATSGDSCT

Target ORF information:

RefSeq Version	NM_199992.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (sema6a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199992.1

ORF Insert Sequence:

ATGCGAGCGC AGGCCCTGCT GCTGTACCTG ACGCTGCTGC AGACGGCTGG AGCCGCTTTC 
CCAGAAGACA CCGAGCCCAT CAGCATCGCA CATGGAAACT ACACTAAACA GTACCCAGTG 
TTCGTGGGAC ACAAACCAGG CAGGAATAAC ACACAGCGAC ACAAACTGGA CATCCAGCTG 
ATCGTCATCA TGAACCGGAC GCTGTATGTG GCTGCGAGGG ATCACATTTA CACGCTGGAC 
ATGGAGACGT CCAGCACAGA GGAGATCTTC TTCAGTAAGA AGCTGACCTG GAAGTCCAGA 
CAGGCGGATG TGGACACGTG CAGGATGAAG GGCAAACACA AGGACGAGTG TCATAACTTC 
ATCAAGGTGC TCCTGCAGCA GAGTGAGGAC TCTCTGTTTG TGTGCGGAAC CAACGCCTTC 
AACCCGTCCT GCAGAACCTA CAGGATGGAC AGTCTGGACG CTCTGGGTGA GGAGATCAGT 
GGGATGGCCC GCTGCCCGTA CGACGCCAAA CACGCCAACG TGGCCCTGTT CGCTGATGGG 
AAGTTATATT CCGCCACAGT CACAGATTTC CTGGCTATCG ACGCAGTGAT CTACCGCAGT 
TTAGGAGACA GTCCCACACT CAGAACCGTC AAACACGACT CCAAATGGCT GAAGGAGCCG 
TATTTCGTGC AGGCGGTGGA TTACGAGGAC TTCATCTACT TCTTCTTTCG GGAAATCGCC 
ATGGAGTACA ACAGCATGGG GAAGGTGGTG TTTCCGCGTG TGGCGCGTGT GTGTAAGAGC 
GACAGCGGCG GCTCTCAGCG TGTGCTGGAG AAACAGTGGA CATCTTTCCT GAAGACGCGC 
CTGAACTGCT CCATTCCTGG AGACTCACAC TTCTACTTCA ACATCCTGCA GGCCGTGACC 
GACGTCATAC ACATCAGCGG ACGAGACGTG GTCATGGCCA CCTTCTCCAC ACCCTACAAC 
AGTATTCCGG GATCAGCTGT GTGTGCGTAT GACATGTCAG AGATCGCTCT GGCCTTCACC 
GGACGCTTCA AAGAGCAGAA ATCACCTGAC TCCACCTGGA CACCTGTGCC CGAGGAGAGG 
GTGCCCAAAC CCAGGCCGGG TGTGTGTGCC GGCTCGTCCT CCGTGGAGAA GTTTAAAGTG 
TCGAGTGAGT TTCCGGACGA TTCTCTGAGC TTCATCAAAG CCCACCCGCT GATGGAGGAG 
GCCGTGCCCG CCATCGCCAA CCGGCCCTGG TTCCTCAAGA CCATGGTGAG GTACCGGCTC 
ACTCGTATCG CAGTGGATAA CGCCGCCGGG CCGCACAGTA ACCATACGGT GGTCTTCCTG 
GGCTCTGAGC GAGGAATCGT CCTGAAGTTT CTGGCTAAGA TCCGCAGCGG CGCCCTGAAC 
GACAGCCTGT TCCTGGAGGA GCTGAACGTC TACAACGCAG AGAAGTGCAG TATTGACGGT 
GTGGAGGATA AGCGCATCAT CAGTATGCAG ATCGACAGCC GCAGCCGATC TCTGTTCGTG 
GCCTTTAGCT CCTGTGTGGT TCGAGTCCCG CTCGCGCGCT GCCAGCGCCA TGGAAAGTGC 
AAGAAGTCCT GTATCGCCTC CAGAGACCCG TACTGCGGCT GGGTGTCCGA GGGCGCCTGC 
AGAGAAATCA CATCTGATGC AAAATGGCCG TTCGAGCAGG ACGTGGAGCA GGGAAACACA 
GATGGACTGG GCGACTGCCA AAATTCGTTC GTGGCTCTGA ACGGCCGTGT CTCCTCCCCC 
TCAGTGTCTC CAGCAGTGGT CAGTGTTCCT CTGGGTCGGG GTCGAGTGCT GAAGGATCAC 
CAGGAGCCGC TGGTGTCCGG GTCCTCAGAG ACGCAGCAGG AGAGCGCGGG AGTGATCCGC 
GAGACCTTCC ACCGGGACCG GGATCAGATG GTTCCAGTGA CGCTGTTGGC CATCGCTGTG 
ATCCTGGCCT TCAGCATGGG CGCCATCTTC AGCGGGATCC TGGTGTACTG TGTGTGTGAC 
CACCACCGCC GGCGGGAGTT CCCCATGTCA GGACGCAAAG AGAAGGACTG TGCGGCGTCC 
CGCCGCGGCT CCATGAACAG TGTGACCAAG CTGACGGGGC TGTTCGAGAC GCAGGCTAAA 
GACGGGCGTG CAGAGCAGAT GCTGACCCCG CTGATGAACG GCCGGCTGGT CAACGGGCGG 
ATGCTGATTA AAGCGGAGCA GCACCTGGAC CTGTCCGCCC TGCCCACACC CGAGTCCACA 
CCACTGCAGC CACGCCGCAA GCCTAGCAGG GGGAGCCGAG AGTGGGAGCG CAACCAGAAC 
CTGATCAACG CCTGCACCAA AGAGCTGTCA TCAATGGGGT CACCTGTGAT CCCGACAGAG 
CTGCCGCTTC GGGCATCCCC GGGCCACATC TCCAGTGTGG TGGTGCTGCC ACTGCCACCA 
CATCAGTCGT ACCAGCACGA GTACGTGGAG CATCCGCAGC AGCGCCCTGA CGATCCAGCG 
GCCACGCTGG AGTTCCAGAC GCTGCGGAGT CCGTGCCGTG GTGCAGAGGA GGAGGTGGTG 
CCGCCACGAG TGCCCCAGCG GGAGGCGTCC CTGGTGCCAC CGGCCGTCCC ACAGATGGGC 
AAACGGCTAG ACATGCACTC CTCCATGTAC GCCCGCGGGT TCCCCATGAG CTCCGGCCTG 
AAGAAGCAGC ACAACACCAA CTCCTCCAAC TCCTCACACA TGTCCAGGAA CCACAGCTTC 
CGTGTGGAGA CGCCTCCGCC CGCCCCGCAG CGCGTGGACT CCATGCAGGT CTCCTCTCCG 
GGCCTCGGCC TCTCCCACCA CCCCAGCCTC AGCTCTTTCG GCTCTCTGTC TCGCTGCGGA 
GCCCGGCAGA TGAAGCCCGA CGTGCCGCCC AAACCCTCGG CCGTGTCCTA CTGCAGCAAA 
GCCACATCCG GAGACTCCTG CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 07)
Emerson SE,St Clair RM,Waldron AL,Bruno SR,Duong A,Driscoll HE,Ballif BA,McFarlane S,Ebert AM

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Sema6a and Plxna2 mediate spatially regulated repulsion within the developing eye to promote eye vesicle cohesion.
Development (Cambridge, England)141(12)2473-82(2014 Jun)
Ebert AM,Childs SJ,Hehr CL,Cechmanek PB,McFarlane S

Neuronal expression of class 6 semaphorins in zebrafish.
Gene expression patterns : GEP12(3-4)117-22(2012 Mar-Apr)
Ebert AM,Lamont RE,Childs SJ,McFarlane S

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sema6a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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