Rfwd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rfwd2
Entrez Gene ID	360860
Full Name	ring finger and WD repeat domain 2
Synonyms	Cop1,RGD1304773
General protein information	Preferred Names ring finger and WD repeat domain 2 Names E3 ubiquitin-protein ligase RFWD2 ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025126.1	NP_001020297.1	E3 ubiquitin-protein ligase RFWD2
XM_006250114.3	XP_006250176.1	E3 ubiquitin-protein ligase RFWD2 isoform X2
XM_006250113.3	XP_006250175.1	E3 ubiquitin-protein ligase RFWD2 isoform X1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0771100	NP_001048254.1
Pan troglodytes (chimpanzee)	RFWD2	XP_514018.2
Danio rerio (zebrafish)	rfwd2	NP_001083011.1
Bos taurus (cattle)	RFWD2	NP_001096726.1
Rattus norvegicus (Norway rat)	Rfwd2	NP_001020297.1
Canis lupus familiaris (dog)	RFWD2	XP_537181.2
Homo sapiens (human)	RFWD2	NP_071902.2
Macaca mulatta (Rhesus monkey)	RFWD2	XP_001104796.1
Mus musculus (house mouse)	Rfwd2	NP_036061.1
Gallus gallus (chicken)	RFWD2	XP_004943320.1
Xenopus tropicalis (tropical clawed frog)	rfwd2	NP_001016199.1
Arabidopsis thaliana (thale cress)	COP1	NP_180854.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of biological pathways associated with a 1.37 Mbp genomic region protective of hypertension in Dahl S rats.
Cowley AW Jr, Moreno C, Jacob HJ, Peterson CB, Stingo FC, Ahn KW, Liu P, Vannucci M, Laud PW, Reddy P, Lazar J, Evans L, Yang C, Kurth T, Liang M
Physiological genomics46(11)398-410(2014 Jun)

E3 ubiquitin ligase COP1 regulates the stability and functions of MTA1.
Li DQ, Ohshiro K, Reddy SD, Pakala SB, Lee MH, Zhang Y, Rayala SK, Kumar R
Proceedings of the National Academy of Sciences of the United States of America106(41)17493-8(2009 Oct)

COP1 functions as a FoxO1 ubiquitin E3 ligase to regulate FoxO1-mediated gene expression.
Kato S, Ding J, Pisck E, Jhala US, Du K
The Journal of biological chemistry283(51)35464-73(2008 Dec)

Cytosol-derived proteins are sufficient for Arp2/3 recruitment and ARF/coatomer-dependent actin polymerization on Golgi membranes.
Chen JL, Lacomis L, Erdjument-Bromage H, Tempst P, Stamnes M
FEBS letters566(1-3)281-6(2004 May)

Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase.
Wertz IE, O'Rourke KM, Zhang Z, Dornan D, Arnott D, Deshaies RJ, Dixit VM
Science (New York, N.Y.)303(5662)1371-4(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rfwd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rfwd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07285	NM_001025126.1 Latest version!	Rattus norvegicus COP1, E3 ubiquitin ligase (Cop1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa35530	XM_006250113.3 Latest version!	Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa35531	XM_006250114.3 Latest version!	Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rfwd2 ( NM_001025126.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020297.1 Rattus norvegicus COP1, E3 ubiquitin ligase (Cop1), mRNA.

Price & Availability↑

CloneID	ORa07285
Clone ID Related Accession (Same CDS sequence)	NM_001025126.1
Accession Version	NM_001025126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522080000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RFWD2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091284.1. On Jul 1, 2005 this sequence version replaced XM_341137.2. ##Evidence-Data-START## Transcript exon combination :: BC091284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGGCT TGTACTCCCC CGTCAGTGAA GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCGC ACAGTAGCAT TATTGACTCT ACAGAATACA GCCAACCTCC AGGCTTCAGT 
GGGCCGTCTC AGACAAAGAA ACAGCCTTGG TATAACAGCA CATTAGCATC AAGACGAAAG 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAGTGTTATT TTTCTACAAG GATGTCTCGT 
ATCTCAGATG ACAGTAGAAC TGCAAGCCAG TTAGATGAAT TTCAAGAATG CTTGTCCAAA 
TTTACTCGAT ATAACTCTGT AAGACCGTTG GCCACATTGT CTTATGCTAG TGATCTGTAT 
AATGGTTCCA GCATAGTTTC TAGTATTGAA TTTGACCGGG ATTGTGACTA CTTTGCAATT 
GCTGGAGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATCCA GGATGCAGTG 
GATATTCATT ACCCTGAGAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAACCT GCTAGCCAGC AGTGATTATG AAGGCACCGT TATACTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAGC ATGAAAAGAG GTGTTGGAGT 
GTCGACTTTA ATTTGATGGA TCCCAAACTT CTGGCTTCAG GTTCTGATGA TGCAAAAGTG 
AAGCTTTGGT CCACCAATTT AGACAATTCT GTGGCAAGCA TCGAGGCAAA AGCTAATGTG 
TGTTGTGTGA AGTTCAGCCC CTCCTCCAGG TACCATCTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTACGATCT TCGTAACACT AAACAGCCAA TAATGGTGTT CAAGGGGCAC 
CGAAAAGCAG TGTCTTATGC CAAGTTTGTG AGTGGTGAAG AAATTGTCTC TGCCTCAACA 
GACAGCCAAC TAAAACTGTG GAATGTGGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CATATTAATG AAAAAAACTT TGTAGGCCTT GCTTCCAATG GAGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCCCTCTA CCTGTACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CAGTTAAGAG TGTATTAGAT AAAGACCGGA AAGAAGATGA CACCAATGAA 
TTTGTCAGTG CTGTGTGTTG GCGGGCACTA TCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGCC AGGGTACAAT TAAGGTGCTA GAATTGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020297.1
CDS	184..1485
Translation	MSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGPSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALSDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	NM_001025126.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus COP1, E3 ubiquitin ligase (Cop1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025126.1

ORF Insert Sequence:

ATGAGTGGCT TGTACTCCCC CGTCAGTGAA GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCGC ACAGTAGCAT TATTGACTCT ACAGAATACA GCCAACCTCC AGGCTTCAGT 
GGGCCGTCTC AGACAAAGAA ACAGCCTTGG TATAACAGCA CATTAGCATC AAGACGAAAG 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAGTGTTATT TTTCTACAAG GATGTCTCGT 
ATCTCAGATG ACAGTAGAAC TGCAAGCCAG TTAGATGAAT TTCAAGAATG CTTGTCCAAA 
TTTACTCGAT ATAACTCTGT AAGACCGTTG GCCACATTGT CTTATGCTAG TGATCTGTAT 
AATGGTTCCA GCATAGTTTC TAGTATTGAA TTTGACCGGG ATTGTGACTA CTTTGCAATT 
GCTGGAGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATCCA GGATGCAGTG 
GATATTCATT ACCCTGAGAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAACCT GCTAGCCAGC AGTGATTATG AAGGCACCGT TATACTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAGC ATGAAAAGAG GTGTTGGAGT 
GTCGACTTTA ATTTGATGGA TCCCAAACTT CTGGCTTCAG GTTCTGATGA TGCAAAAGTG 
AAGCTTTGGT CCACCAATTT AGACAATTCT GTGGCAAGCA TCGAGGCAAA AGCTAATGTG 
TGTTGTGTGA AGTTCAGCCC CTCCTCCAGG TACCATCTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTACGATCT TCGTAACACT AAACAGCCAA TAATGGTGTT CAAGGGGCAC 
CGAAAAGCAG TGTCTTATGC CAAGTTTGTG AGTGGTGAAG AAATTGTCTC TGCCTCAACA 
GACAGCCAAC TAAAACTGTG GAATGTGGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CATATTAATG AAAAAAACTT TGTAGGCCTT GCTTCCAATG GAGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCCCTCTA CCTGTACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CAGTTAAGAG TGTATTAGAT AAAGACCGGA AAGAAGATGA CACCAATGAA 
TTTGTCAGTG CTGTGTGTTG GCGGGCACTA TCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGCC AGGGTACAAT TAAGGTGCTA GAATTGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rfwd2 ( XM_006250113.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250175.1 Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35530
Clone ID Related Accession (Same CDS sequence)	XM_006250113.3
Accession Version	XM_006250113.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGTA GCCGCCAGGC CGGTTCGGGT TCCGCTGGGA CGAGCCCTGG CTCCTCAGCC 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTATCCTCGT CTCCGTCGCC GCCTTCCGTG 
GCGGCCTCGG CGGCGACGCT GGTGTCCGGC GGGGTAGCTC CAGCCGCGGG CTCCGGCGGC 
CTCGGCGGCC CGGGGCGGCC TGTGCTAGTG GCGGCCGCGG TGTCTGGCAG CGCGAGCGCC 
GGCGGGGCAG TGTCCGCGGG CCTGTCCCGG CTCAGCTGCG CTGCCAGGCC CAGCGCCGGC 
GTTGGAGGGA GCAGCTCCAG CCTCGGCAGC AGCAGTAGGA AGCGACCTCT GATCGCCCCG 
CTTTGCAACG GGCTCCTCAA CTCCTACGAG GACAAAAGCA ACGACTTCGT CTGCCCCATC 
TGCTTTGATA TGATTGAAGA GGCATACATG ACAAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGACAATAAT AGATGTCCCA AGTGTAATTA TGTTGTGGAC 
AATATCGACC ATCTCTATCC TAATTTCTTG GTGAATGAAC TCATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTGGAC CACTCAGTGA GTAGCACCAA TGGGCATAGG 
TGGCAAATAT TTCAAGATCT GCTAGGAACT GATCAAGATA ATCTTGATTT GGCTAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAAAAACAAC TGGAAGCAGA ATCACATGCA 
GCTCAGCTAC AGATCCTTAT GGAATTCCTC AAGGTTGCCA GGAGAAATAA GAGAGAGCAA 
TTGGAACAGA TCCAGAAGGA ACTAAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAG 
ATGAGTGGCT TGTACTCCCC CGTCAGTGAA GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCGC ACAGTAGCAT TATTGACTCT ACAGAATACA GCCAACCTCC AGGCTTCAGT 
GGGCCGTCTC AGACAAAGAA ACAGCCTTGG TATAACAGCA CATTAGCATC AAGACGAAAG 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAGTGTTATT TTTCTACAAG GATGTCTCGT 
ATCTCAGATG ACAGTAGAAC TGCAAGCCAG TTAGATGAAT TTCAAGAATG CTTGTCCAAA 
TTTACTCGAT ATAACTCTGT AAGACCGTTG GCCACATTGT CTTATGCTAG TGATCTGTAT 
AATGGTTCCA GCATAGTTTC TAGTATTGAA TTTGACCGGG ATTGTGACTA CTTTGCAATT 
GCTGGAGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATCCA GGATGCAGTG 
GATATTCATT ACCCTGAGAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAACCT GCTAGCCAGC AGTGATTATG AAGGCACCGT TATACTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAGC ATGAAAAGAG GTGTTGGAGT 
GTCGACTTTA ATTTGATGGA TCCCAAACTT CTGGCTTCAG GTTCTGATGA TGCAAAAGTG 
AAGCTTTGGT CCACCAATTT AGACAATTCT GTGGCAAGCA TCGAGGCAAA AGCTAATGTG 
TGTTGTGTGA AGTTCAGCCC CTCCTCCAGG TACCATCTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTACGATCT TCGTAACACT AAACAGCCAA TAATGGTGTT CAAGGGGCAC 
CGAAAAGCAG TGTCTTATGC CAAGTTTGTG AGTGGTGAAG AAATTGTCTC TGCCTCAACA 
GACAGCCAAC TAAAACTGTG GAATGTGGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CATATTAATG AAAAAAACTT TGTAGGCCTT GCTTCCAATG GAGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCCCTCTA CCTGTACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CAGTTAAGAG TGTATTAGAT AAAGACCGGA AAGAAGATGA CACCAATGAA 
TTTGTCAGTG CTGTGTGTTG GCGGGCACTA TCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGCC AGGGTACAAT TAAGGTGCTA GAATTGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250175.1
CDS	533..2734
Translation	MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAASAATLVSGGVAPAAGSGGLGGPGRPVLVAAAVSGSASAGGAVSAGLSRLSCAARPSAGVGGSSSSLGSSSRKRPLIAPLCNGLLNSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGPSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALSDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_006250113.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250113.3

ORF Insert Sequence:

ATGTCAGGTA GCCGCCAGGC CGGTTCGGGT TCCGCTGGGA CGAGCCCTGG CTCCTCAGCC 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTATCCTCGT CTCCGTCGCC GCCTTCCGTG 
GCGGCCTCGG CGGCGACGCT GGTGTCCGGC GGGGTAGCTC CAGCCGCGGG CTCCGGCGGC 
CTCGGCGGCC CGGGGCGGCC TGTGCTAGTG GCGGCCGCGG TGTCTGGCAG CGCGAGCGCC 
GGCGGGGCAG TGTCCGCGGG CCTGTCCCGG CTCAGCTGCG CTGCCAGGCC CAGCGCCGGC 
GTTGGAGGGA GCAGCTCCAG CCTCGGCAGC AGCAGTAGGA AGCGACCTCT GATCGCCCCG 
CTTTGCAACG GGCTCCTCAA CTCCTACGAG GACAAAAGCA ACGACTTCGT CTGCCCCATC 
TGCTTTGATA TGATTGAAGA GGCATACATG ACAAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGACAATAAT AGATGTCCCA AGTGTAATTA TGTTGTGGAC 
AATATCGACC ATCTCTATCC TAATTTCTTG GTGAATGAAC TCATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTGGAC CACTCAGTGA GTAGCACCAA TGGGCATAGG 
TGGCAAATAT TTCAAGATCT GCTAGGAACT GATCAAGATA ATCTTGATTT GGCTAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAAAAACAAC TGGAAGCAGA ATCACATGCA 
GCTCAGCTAC AGATCCTTAT GGAATTCCTC AAGGTTGCCA GGAGAAATAA GAGAGAGCAA 
TTGGAACAGA TCCAGAAGGA ACTAAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAG 
ATGAGTGGCT TGTACTCCCC CGTCAGTGAA GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCGC ACAGTAGCAT TATTGACTCT ACAGAATACA GCCAACCTCC AGGCTTCAGT 
GGGCCGTCTC AGACAAAGAA ACAGCCTTGG TATAACAGCA CATTAGCATC AAGACGAAAG 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAGTGTTATT TTTCTACAAG GATGTCTCGT 
ATCTCAGATG ACAGTAGAAC TGCAAGCCAG TTAGATGAAT TTCAAGAATG CTTGTCCAAA 
TTTACTCGAT ATAACTCTGT AAGACCGTTG GCCACATTGT CTTATGCTAG TGATCTGTAT 
AATGGTTCCA GCATAGTTTC TAGTATTGAA TTTGACCGGG ATTGTGACTA CTTTGCAATT 
GCTGGAGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATCCA GGATGCAGTG 
GATATTCATT ACCCTGAGAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAACCT GCTAGCCAGC AGTGATTATG AAGGCACCGT TATACTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAGC ATGAAAAGAG GTGTTGGAGT 
GTCGACTTTA ATTTGATGGA TCCCAAACTT CTGGCTTCAG GTTCTGATGA TGCAAAAGTG 
AAGCTTTGGT CCACCAATTT AGACAATTCT GTGGCAAGCA TCGAGGCAAA AGCTAATGTG 
TGTTGTGTGA AGTTCAGCCC CTCCTCCAGG TACCATCTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTACGATCT TCGTAACACT AAACAGCCAA TAATGGTGTT CAAGGGGCAC 
CGAAAAGCAG TGTCTTATGC CAAGTTTGTG AGTGGTGAAG AAATTGTCTC TGCCTCAACA 
GACAGCCAAC TAAAACTGTG GAATGTGGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CATATTAATG AAAAAAACTT TGTAGGCCTT GCTTCCAATG GAGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCCCTCTA CCTGTACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CAGTTAAGAG TGTATTAGAT AAAGACCGGA AAGAAGATGA CACCAATGAA 
TTTGTCAGTG CTGTGTGTTG GCGGGCACTA TCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGCC AGGGTACAAT TAAGGTGCTA GAATTGGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rfwd2 ( XM_006250114.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250176.1 Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35531
Clone ID Related Accession (Same CDS sequence)	XM_006250114.3
Accession Version	XM_006250114.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGTA GCCGCCAGGC CGGTTCGGGT TCCGCTGGGA CGAGCCCTGG CTCCTCAGCC 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTATCCTCGT CTCCGTCGCC GCCTTCCGTG 
GCGGCCTCGG CGGCGACGCT GGTGTCCGGC GGGGTAGCTC CAGCCGCGGG CTCCGGCGGC 
CTCGGCGGCC CGGGGCGGCC TGTGCTAGTG GCGGCCGCGG TGTCTGGCAG CGCGAGCGCC 
GGCGGGGCAG TGTCCGCGGG CCTGTCCCGG CTCAGCTGCG CTGCCAGGCC CAGCGCCGGC 
GTTGGAGGGA GCAGCTCCAG CCTCGGCAGC AGCAGTAGGA AGCGACCTCT GATCGCCCCG 
CTTTGCAACG GGCTCCTCAA CTCCTACGAG GACAAAAGCA ACGACTTCGT CTGCCCCATC 
TGCTTTGATA TGATTGAAGA GGCATACATG ACAAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGACAATAAT AGATGTCCCA AGTGTAATTA TGTTGTGGAC 
AATATCGACC ATCTCTATCC TAATTTCTTG GTGAATGAAC TCATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTGGAC CACTCAAATG GGCATAGGTG GCAAATATTT 
CAAGATCTGC TAGGAACTGA TCAAGATAAT CTTGATTTGG CTAATGTCAA CCTCATGTTG 
GAATTACTAG TGCAGAAGAA AAAACAACTG GAAGCAGAAT CACATGCAGC TCAGCTACAG 
ATCCTTATGG AATTCCTCAA GGTTGCCAGG AGAAATAAGA GAGAGCAATT GGAACAGATC 
CAGAAGGAAC TAAGTGTTTT GGAAGAGGAT ATTAAAAGAG TGGAAGAGAT GAGTGGCTTG 
TACTCCCCCG TCAGTGAAGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCGCAC 
AGTAGCATTA TTGACTCTAC AGAATACAGC CAACCTCCAG GCTTCAGTGG GCCGTCTCAG 
ACAAAGAAAC AGCCTTGGTA TAACAGCACA TTAGCATCAA GACGAAAGCG ACTCACTGCT 
CATTTTGAAG ACTTAGAGCA GTGTTATTTT TCTACAAGGA TGTCTCGTAT CTCAGATGAC 
AGTAGAACTG CAAGCCAGTT AGATGAATTT CAAGAATGCT TGTCCAAATT TACTCGATAT 
AACTCTGTAA GACCGTTGGC CACATTGTCT TATGCTAGTG ATCTGTATAA TGGTTCCAGC 
ATAGTTTCTA GTATTGAATT TGACCGGGAT TGTGACTACT TTGCAATTGC TGGAGTTACA 
AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATCCAGG ATGCAGTGGA TATTCATTAC 
CCTGAGAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT 
AAGAACCTGC TAGCCAGCAG TGATTATGAA GGCACCGTTA TACTATGGGA TGGATTCACA 
GGACAGAGGT CAAAGGTCTA TCAGGAGCAT GAAAAGAGGT GTTGGAGTGT CGACTTTAAT 
TTGATGGATC CCAAACTTCT GGCTTCAGGT TCTGATGATG CAAAAGTGAA GCTTTGGTCC 
ACCAATTTAG ACAATTCTGT GGCAAGCATC GAGGCAAAAG CTAATGTGTG TTGTGTGAAG 
TTCAGCCCCT CCTCCAGGTA CCATCTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC 
TACGATCTTC GTAACACTAA ACAGCCAATA ATGGTGTTCA AGGGGCACCG AAAAGCAGTG 
TCTTATGCCA AGTTTGTGAG TGGTGAAGAA ATTGTCTCTG CCTCAACAGA CAGCCAACTA 
AAACTGTGGA ATGTGGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA TATTAATGAA 
AAAAACTTTG TAGGCCTTGC TTCCAATGGA GATTATATAG CTTGTGGAAG TGAGAACAAC 
TCCCTCTACC TGTACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACA 
GTTAAGAGTG TATTAGATAA AGACCGGAAA GAAGATGACA CCAATGAATT TGTCAGTGCT 
GTGTGTTGGC GGGCACTATC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGCCAG 
GGTACAATTA AGGTGCTAGA ATTGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250176.1
CDS	533..2722
Translation	MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAASAATLVSGGVAPAAGSGGLGGPGRPVLVAAAVSGSASAGGAVSAGLSRLSCAARPSAGVGGSSSSLGSSSRKRPLIAPLCNGLLNSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQIFQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGPSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALSDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_006250114.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (Rfwd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250114.3

ORF Insert Sequence:

ATGTCAGGTA GCCGCCAGGC CGGTTCGGGT TCCGCTGGGA CGAGCCCTGG CTCCTCAGCC 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTATCCTCGT CTCCGTCGCC GCCTTCCGTG 
GCGGCCTCGG CGGCGACGCT GGTGTCCGGC GGGGTAGCTC CAGCCGCGGG CTCCGGCGGC 
CTCGGCGGCC CGGGGCGGCC TGTGCTAGTG GCGGCCGCGG TGTCTGGCAG CGCGAGCGCC 
GGCGGGGCAG TGTCCGCGGG CCTGTCCCGG CTCAGCTGCG CTGCCAGGCC CAGCGCCGGC 
GTTGGAGGGA GCAGCTCCAG CCTCGGCAGC AGCAGTAGGA AGCGACCTCT GATCGCCCCG 
CTTTGCAACG GGCTCCTCAA CTCCTACGAG GACAAAAGCA ACGACTTCGT CTGCCCCATC 
TGCTTTGATA TGATTGAAGA GGCATACATG ACAAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGACAATAAT AGATGTCCCA AGTGTAATTA TGTTGTGGAC 
AATATCGACC ATCTCTATCC TAATTTCTTG GTGAATGAAC TCATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTGGAC CACTCAAATG GGCATAGGTG GCAAATATTT 
CAAGATCTGC TAGGAACTGA TCAAGATAAT CTTGATTTGG CTAATGTCAA CCTCATGTTG 
GAATTACTAG TGCAGAAGAA AAAACAACTG GAAGCAGAAT CACATGCAGC TCAGCTACAG 
ATCCTTATGG AATTCCTCAA GGTTGCCAGG AGAAATAAGA GAGAGCAATT GGAACAGATC 
CAGAAGGAAC TAAGTGTTTT GGAAGAGGAT ATTAAAAGAG TGGAAGAGAT GAGTGGCTTG 
TACTCCCCCG TCAGTGAAGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCGCAC 
AGTAGCATTA TTGACTCTAC AGAATACAGC CAACCTCCAG GCTTCAGTGG GCCGTCTCAG 
ACAAAGAAAC AGCCTTGGTA TAACAGCACA TTAGCATCAA GACGAAAGCG ACTCACTGCT 
CATTTTGAAG ACTTAGAGCA GTGTTATTTT TCTACAAGGA TGTCTCGTAT CTCAGATGAC 
AGTAGAACTG CAAGCCAGTT AGATGAATTT CAAGAATGCT TGTCCAAATT TACTCGATAT 
AACTCTGTAA GACCGTTGGC CACATTGTCT TATGCTAGTG ATCTGTATAA TGGTTCCAGC 
ATAGTTTCTA GTATTGAATT TGACCGGGAT TGTGACTACT TTGCAATTGC TGGAGTTACA 
AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATCCAGG ATGCAGTGGA TATTCATTAC 
CCTGAGAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT 
AAGAACCTGC TAGCCAGCAG TGATTATGAA GGCACCGTTA TACTATGGGA TGGATTCACA 
GGACAGAGGT CAAAGGTCTA TCAGGAGCAT GAAAAGAGGT GTTGGAGTGT CGACTTTAAT 
TTGATGGATC CCAAACTTCT GGCTTCAGGT TCTGATGATG CAAAAGTGAA GCTTTGGTCC 
ACCAATTTAG ACAATTCTGT GGCAAGCATC GAGGCAAAAG CTAATGTGTG TTGTGTGAAG 
TTCAGCCCCT CCTCCAGGTA CCATCTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC 
TACGATCTTC GTAACACTAA ACAGCCAATA ATGGTGTTCA AGGGGCACCG AAAAGCAGTG 
TCTTATGCCA AGTTTGTGAG TGGTGAAGAA ATTGTCTCTG CCTCAACAGA CAGCCAACTA 
AAACTGTGGA ATGTGGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA TATTAATGAA 
AAAAACTTTG TAGGCCTTGC TTCCAATGGA GATTATATAG CTTGTGGAAG TGAGAACAAC 
TCCCTCTACC TGTACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACA 
GTTAAGAGTG TATTAGATAA AGACCGGAAA GAAGATGACA CCAATGAATT TGTCAGTGCT 
GTGTGTTGGC GGGCACTATC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGCCAG 
GGTACAATTA AGGTGCTAGA ATTGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of biological pathways associated with a 1.37 Mbp genomic region protective of hypertension in Dahl S rats.
Physiological genomics46(11)398-410(2014 Jun)
Cowley AW Jr,Moreno C,Jacob HJ,Peterson CB,Stingo FC,Ahn KW,Liu P,Vannucci M,Laud PW,Reddy P,Lazar J,Evans L,Yang C,Kurth T,Liang M

E3 ubiquitin ligase COP1 regulates the stability and functions of MTA1.
Proceedings of the National Academy of Sciences of the United States of America106(41)17493-8(2009 Oct)
Li DQ,Ohshiro K,Reddy SD,Pakala SB,Lee MH,Zhang Y,Rayala SK,Kumar R

COP1 functions as a FoxO1 ubiquitin E3 ligase to regulate FoxO1-mediated gene expression.
The Journal of biological chemistry283(51)35464-73(2008 Dec)
Kato S,Ding J,Pisck E,Jhala US,Du K

Cytosol-derived proteins are sufficient for Arp2/3 recruitment and ARF/coatomer-dependent actin polymerization on Golgi membranes.
FEBS letters566(1-3)281-6(2004 May)
Chen JL,Lacomis L,Erdjument-Bromage H,Tempst P,Stamnes M

Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase.
Science (New York, N.Y.)303(5662)1371-4(2004 Feb)
Wertz IE,O'Rourke KM,Zhang Z,Dornan D,Arnott D,Deshaies RJ,Dixit VM

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Rfwd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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