Sptb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptb
Entrez Gene ID	314251
Full Name	spectrin, beta, erythrocytic
Synonyms	Spnb1
General protein information	Preferred Names spectrin, beta, erythrocytic Names spectrin beta chain, erythrocytic erythroid spectrin beta spectrin beta 1 spectrin beta chain, erythrocyte
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_006240244.3	XP_006240306.1	spectrin beta chain, erythrocytic isoform X1
XM_017594143.1	XP_017449632.1	spectrin beta chain, erythrocytic isoform X1
XM_017594142.1	XP_017449631.1	spectrin beta chain, erythrocytic isoform X1
NM_212522.1	NP_997687.1	spectrin beta chain, erythrocytic

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SPTB	XP_004941320.1
Macaca mulatta (Rhesus monkey)	SPTB	XP_002805136.1
Rattus norvegicus (Norway rat)	Sptb	NP_997687.1
Xenopus tropicalis (tropical clawed frog)	LOC100489265	XP_004917321.1
Canis lupus familiaris (dog)	SPTB	NP_001207410.1
Homo sapiens (human)	SPTB	NP_001020029.1
Bos taurus (cattle)	SPTB	XP_003586637.2
Mus musculus (house mouse)	Sptb	NP_038703.3

Gene Ontology
Bibliography

Related articles in PubMed

The actin binding domain of βI-spectrin regulates the morphological and functional dynamics of dendritic spines.
Nestor MW, Cai X, Stone MR, Bloch RJ, Thompson SM
PloS one6(1)e16197(2011 Jan)

The carboxyterminal EF domain of erythroid alpha-spectrin is necessary for optimal spectrin-actin binding.
Korsgren C, Lux SE
Blood116(14)2600-7(2010 Oct)

Novel beta-spectrin mutations in hereditary spherocytosis associated with decreased levels of mRNA.
Maciag M, Płochocka D, Adamowicz-Salach A, Burzyńska B
British journal of haematology146(3)326-32(2009 Aug)

Targeted deletion of alpha-adducin results in absent beta- and gamma-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice.
Robledo RF, Ciciotte SL, Gwynn B, Sahr KE, Gilligan DM, Mohandas N, Peters LL
Blood112(10)4298-307(2008 Nov)

Identification and functional characterization of protein 4.1R and actin-binding sites in erythrocyte beta spectrin: regulation of the interactions by phosphatidylinositol-4,5-bisphosphate.
An X, Debnath G, Guo X, Liu S, Lux SE, Baines A, Gratzer W, Mohandas N
Biochemistry44(31)10681-8(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sptb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05792	NM_212522.1 Latest version!	Rattus norvegicus spectrin, beta, erythrocytic (Sptb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa29427	XM_006240244.3 Latest version!	Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa29427	XM_017594143.1 Latest version!	Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa29427	XM_017594142.1 Latest version!	Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptb ( NM_212522.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997687.1 Rattus norvegicus spectrin, beta, erythrocytic (Sptb), mRNA.

Price & Availability↑

CloneID	ORa05792
Clone ID Related Accession (Same CDS sequence)	NM_212522.1
Accession Version	NM_212522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506700800000
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, erythrocytic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY238343.1. On Aug 30, 2004 this sequence version replaced XM_234322.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY238343.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGACATCAG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATACAG CCGGATTAAT 
GCCCGCTGGG ACGCCCCAGA TGATGAACTG GACAACGATA GCAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTTCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA TCTCTACAAG 
GACCTGCGGG ATGGACGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCCGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAGCAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATCGTGGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACGATTA TCCTCCGATT CCAGATTCAA 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTT 
CTGTGGTGCC AGATGAAGAC AGCCGGCTAC CCCCAAGTCA ACGTAACCAA CTTCACCTCC 
AGCTGGAAGG ACGGCCTGGC CTTCAATGCC CTAATTCATA AACACCGGCC TGACCTCATT 
GACTTTGACA AGCTAAAGGA CTCCAATGCC CGGCATAACT TGGAGCACGC ATTTGATGTG 
GCCGAGCGTC AGCTGGGCAT CATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCAGACGAGA AGTCCATCAT CACCTACGTG GTAGCATTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTAG CGGTGGAGGG CAAGAGAGTC GGCAAGGTCA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACCTG GATTGAGCAA 
ACCATTAGCG TCCTGAACAG CCGCAAGTTT GCCAACTCAT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGCACTGTG GAGAAGCCAC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TCCTCCTGTT CACTATCCAA TCCCGAATGA GAGCCAACAA TCAGAAAGTG 
TACACGCCCC ACGATGGGAA GCTAGTGTCT GACATCAACA GGGCCTGGGA GAGCCTAGAG 
GAAGCAGAAT ACCAAAGGGA GCTGGCCCTG AGGAGCGAGC TCATTCGGCA GGAGAAACTG 
GAGCAGCTGG CGCGGCGCTT CGACCGTAAG GCAGCCATGA GAGAGACGTG GCTCAACGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTC GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACGGCTGCCT ATGAGGAGCG GGTGAAGGCA 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGCATCAGT 
GCCAGGAAGA ACAATATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGCGGC TCGAGGCCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGT 
ATCGACTGGA TGGATGAGAT CAAGGCGCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT ACAGAAACAC AAGCTAATGG AAGCCGACAT TGCTATCCAA 
GGGGACAAAG TGAAGACCAT CACCGCGGCC ACCCTGCAGT TCACTGAGGG GAAAGGGTAT 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA ATGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA CGAGGCCGAA AGCTGGATCA AGGAAAAGGA ACAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACG AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTCGAGG ACGAGCTCCG TGGGCTCGAT GCCCATCTGA AGCAGATCTT CCAGGAGGCT 
GAGGACATGG TTGCACAAAA GCAGTTTGGA CATCCACAGA TCGAGACCCG TGTCAAAGAA 
GTATCGGCTC AGTGGGACCA CTTGAAAGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTTTC CGGTGAAGAT GTGGGGCAAG ACGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTTCTAGAG GAGCTGGAGG AGAGCCGCGG GGTGATGGAG 
CACTTAGAGA ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTGACA 
AACCGTCTAC AGGCACTCAG GAAGCTCTAC CAGCAGGTGA TGGCCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACTGTGT TTGGAGAGTC AGACGCCTGT 
GAGCTGTGGA TGACTGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTTGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGCGT GAACCTTGCG GCCAACAACC TGGTAGAGAG CGGCCACCCA 
CGCAGTGGGG AGGTGAAACA ATATCAGGAC CGCCTAAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCGGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAAACCAG CAAGTGGATC GTGGACAAGA CGAAGGTTGT GGAGTCTACC 
AAGGACTTGG GGCAGGACCT AGCAGGAGTC ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGACG TATTGGCCAT CCGGGATCGT GTGAGCGCCT TGGAACGGGA GTCGCAGTAC 
TTGATGGAGT CACACCCTGA CCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTCGAG 
AAGATGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGTGAGGCC 
AGCAAGCTGC AGGCTTTCCT ACAAGACCTG GATGACTTCA AGGCCTGGCT GTCGATGGCC 
CAAAAGGCAG TGGCCTCTGA GGACATGCCT GAGTCACTCC CAGAGGCTGA GCAACTCCTA 
CAGCAGCATG CAGCCATTAA AGAGGAGATT GATGCTCACA AAGACGATTA CCACCGGGTC 
AAGGCCTCTG GGGAAAAAGT GATCGAAGGT CAAACTGACC CAGATTACCA GCTTCTGGGC 
CAGCGACTGG AGGGTCTAGA TACTGACTGG GATGCTCTGT GGCGGATGTG GGAGAGCCGA 
GGGAACACCC TCACCCAGTG CCTGGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACC TGGAGCCCCC AGACTCCCTA 
GCAGCTGCAG AGGCCGGCAT CCGAAAGTTT GAGGATTTCT TAGTGTCTAT GGAAAACAAC 
CGAGATAAAG TCCTGAGTCC TGTGGACTCC GGAAACATGC TGGTGTCTGA GGGCAACCTG 
TACTCGAACA AGATCAAAGA GAAGGTGCAG CTGATTGAAG ACAGGCACAT GAAGAACAAT 
GAGAAAGCCC AGGAGGTTAC AGCTCTACTG AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACATC 
TCCTATGACG AAGCCCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC ATTTATGGCA 
GAGCTGGCTT CACACCAAGG GTGGCTTGAG AACATCGTCG CGGAGGGAAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAC GGATGTGGTG TCAGAAAGGC TGGAAGCCCT GCACAAGCTC 
TGGGATGAGC TGCAGACCAC CACAAAGGCG AAGACGGAGC AACTCTCTGC TGCCCGGAGC 
TCTGACCTGC GCTTGCAAAC CCACGCCGAC CTTAGCAAAT GGATCAGCGC CATGGAGGAC 
CAGCTGCGAT CCGACGACCT GGGCAAGGAT CTGACCACTG TCAACCGGAT GTTGGTTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCA 
GAGGCACCCC CACTGGGAGC AGAGGCAGGA GACACGGACA TGAGCATTGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTATC CAAAGCCAAG 
CTACAGATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCGTTG 
GCGCAGTCAG CTGACTATGG CACTAATCTG CAAACAGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT CCTAGGCCAC GCACCACGGG TGGAGGATGT GCTACATCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCTTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCGGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGAAGCTC ACGAGGCACA GCAGTACTAC CTGGATGCGG GCGAGGCTGA AGCGTGGATC 
AGCGAGCAGG AGCTCTACGT GTTCTCTGAC GAACCCCCTA AGGACGAAGA GGGCGCCATC 
GTGATGCTCA AGCGGCACCT TCGGCAGCAG CGCACGGTGG AGGAATATGG AAGGAATATC 
AAACAGCTGG CTGGCCGCGC CCAGAGCCTG CTGTCTGCAG GCCACCCGGA GGGGGAGCAG 
ATCATCAGAC TTCAGGGGCA AGTGGATAAG CAGTATGCCG GGCTGAAGGA CATGGCTGAG 
GAACGGAGGC GGAAGCTAGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTGGAGC AGTGGATTAC GGAGAAAGAG ATGGTGGCCT CATCCCAAGA AATGGGGCAG 
GACTTTGACC ACGTGACTAT GCTCCGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCTATCGGGC AGGAGCGAGT AGACAATGTG AATTCTATCA TTGAGCGTCT CATCGATGCG 
GGCCACAGCG AGGCAGCCAC CATTGCAGAG TGGAAGGATG GGCTGAATGA CATGTGGGCA 
GATCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACGGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGTTG 
CCAGAGGACG TGGGGCTGGA CGCCAGCACA GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AGCTCCACCT CCTGGGTGTG CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC TGGGGAGAAG GCAGATGCTA TCCAGAGCAA GGAGCAGGAG 
GTGTCCGCGG CCTGGCAGGC CCTGCTGGAC GCGTGTGCTG GGCGCCGAGC TCAGCTGGTA 
GATACGGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTCTCCTC GGTGGAACTG 
CTCTTGAAGT ATCACCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AACTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCTTC AGACGAGATC 
CGGGAGAAAT TACAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAAGCC 
CGCTCTGACA GGCTCCACAT GCTGCTGGAG GTGTGCCAGT TCTCGAGGGA CGCTTCTGTG 
GCCGAGGCAT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACG 
GTGGACAGCG TGGAAAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGACA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCTCCCC AGGTCCACCA TGCGGCCACG GAGAGAACAT CCCCGGTCAG TTTTATGTCC 
CGCTTGTCTA GCTCCTGGGA GTCACTGCTG CCAGAGCCTG CTCACCCCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997687.1
CDS	147..6560
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDSSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRISDLYKDLRDGRMLIKLLEVLSGEMLPRPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPQVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFDVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTISVLNSRKFANSLSGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYQRELALRSELIRQEKLEQLARRFDRKAAMRETWLNENQRLVTQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVKALEDLAQELEKENYHDQKRISARKNNILRLWSYLQELLRSRRQRLEATLALQKLFQDMLHSIDWMDEIKAHILSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKTITAATLQFTEGKGYQPCDPQVIQDRVSHLEQCFSELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLKQIFQEAEDMVAQKQFGHPQIETRVKEVSAQWDHLKELAAFRKKDLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKEFLEELEESRGVMEHLENQAQGFPEEFRDSPDVTNRLQALRKLYQQVMAQAELRGHKLQEALDLYTVFGESDACELWMTEKGKWLDQMDIPNTLEDLEVVQHRFDILDQEMKTLMAQIDGVNLAANNLVESGHPRSGEVKQYQDRLNKRWQAFQAVVSEQREAVDSALRVNNYCVDCEETSKWIVDKTKVVESTKDLGQDLAGVIAIQRKLSGLERDVLAIRDRVSALERESQYLMESHPDQKEDIGQRQADVEKMWKGLQDALQGQELSLGEASKLQAFLQDLDDFKAWLSMAQKAVASEDMPESLPEAEQLLQQHAAIKEEIDAHKDDYHRVKASGEKVIEGQTDPDYQLLGQRLEGLDTDWDALWRMWESRGNTLTQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLAAAEAGIRKFEDFLVSMENNRDKVLSPVDSGNMLVSEGNLYSNKIKEKVQLIEDRHMKNNEKAQEVTALLKDNLELQNFLQNCKELTLWINDKLLTSPDISYDEARNLHNKWMKHQAFMAELASHQGWLENIVAEGRQLMAEKPQFTDVVSERLEALHKLWDELQTTTKAKTEQLSAARSSDLRLQTHADLSKWISAMEDQLRSDDLGKDLTTVNRMLVKLKRVEEQVNLRKEELEELFAEAPPLGAEAGDTDMSIEKRFLDLLEPLGRRKKQLELSKAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHAPRVEDVLHRGQELVKAAEIDCQDIEERLGHLQSSWDTLREAAAGRLQRLREAHEAQQYYLDAGEAEAWISEQELYVFSDEPPKDEEGAIVMLKRHLRQQRTVEEYGRNIKQLAGRAQSLLSAGHPEGEQIIRLQGQVDKQYAGLKDMAEERRRKLENMYHLFQLKREADDLEQWITEKEMVASSQEMGQDFDHVTMLRDKFRDFARETGAIGQERVDNVNSIIERLIDAGHSEAATIAEWKDGLNDMWADLLELIDTRMQLLAASYDLHRYFYTGTEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKADAIQSKEQEVSAAWQALLDACAGRRAQLVDTADKFRFFSMVRDLLSWMESIIRQIETQERPRDVSSVELLLKYHQGIKAEINTRAKNFSTCLELGESLLQRQHQASDEIREKLQQVISRRQEMNDKWEARSDRLHMLLEVCQFSRDASVAEAWLIAQEPYLASRDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQTPDRPTEEPGPQEEEGETAGEAPQVHHAATERTSPVSFMSRLSSSWESLLPEPAHPF

Target ORF information:

RefSeq Version	NM_212522.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, erythrocytic (Sptb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212522.1

ORF Insert Sequence:

ATGACATCAG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATACAG CCGGATTAAT 
GCCCGCTGGG ACGCCCCAGA TGATGAACTG GACAACGATA GCAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTTCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA TCTCTACAAG 
GACCTGCGGG ATGGACGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCCGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAGCAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATCGTGGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACGATTA TCCTCCGATT CCAGATTCAA 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTT 
CTGTGGTGCC AGATGAAGAC AGCCGGCTAC CCCCAAGTCA ACGTAACCAA CTTCACCTCC 
AGCTGGAAGG ACGGCCTGGC CTTCAATGCC CTAATTCATA AACACCGGCC TGACCTCATT 
GACTTTGACA AGCTAAAGGA CTCCAATGCC CGGCATAACT TGGAGCACGC ATTTGATGTG 
GCCGAGCGTC AGCTGGGCAT CATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCAGACGAGA AGTCCATCAT CACCTACGTG GTAGCATTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTAG CGGTGGAGGG CAAGAGAGTC GGCAAGGTCA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACCTG GATTGAGCAA 
ACCATTAGCG TCCTGAACAG CCGCAAGTTT GCCAACTCAT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGCACTGTG GAGAAGCCAC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TCCTCCTGTT CACTATCCAA TCCCGAATGA GAGCCAACAA TCAGAAAGTG 
TACACGCCCC ACGATGGGAA GCTAGTGTCT GACATCAACA GGGCCTGGGA GAGCCTAGAG 
GAAGCAGAAT ACCAAAGGGA GCTGGCCCTG AGGAGCGAGC TCATTCGGCA GGAGAAACTG 
GAGCAGCTGG CGCGGCGCTT CGACCGTAAG GCAGCCATGA GAGAGACGTG GCTCAACGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTC GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACGGCTGCCT ATGAGGAGCG GGTGAAGGCA 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGCATCAGT 
GCCAGGAAGA ACAATATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGCGGC TCGAGGCCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGT 
ATCGACTGGA TGGATGAGAT CAAGGCGCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT ACAGAAACAC AAGCTAATGG AAGCCGACAT TGCTATCCAA 
GGGGACAAAG TGAAGACCAT CACCGCGGCC ACCCTGCAGT TCACTGAGGG GAAAGGGTAT 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA ATGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA CGAGGCCGAA AGCTGGATCA AGGAAAAGGA ACAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACG AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTCGAGG ACGAGCTCCG TGGGCTCGAT GCCCATCTGA AGCAGATCTT CCAGGAGGCT 
GAGGACATGG TTGCACAAAA GCAGTTTGGA CATCCACAGA TCGAGACCCG TGTCAAAGAA 
GTATCGGCTC AGTGGGACCA CTTGAAAGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTTTC CGGTGAAGAT GTGGGGCAAG ACGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTTCTAGAG GAGCTGGAGG AGAGCCGCGG GGTGATGGAG 
CACTTAGAGA ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTGACA 
AACCGTCTAC AGGCACTCAG GAAGCTCTAC CAGCAGGTGA TGGCCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACTGTGT TTGGAGAGTC AGACGCCTGT 
GAGCTGTGGA TGACTGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTTGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGCGT GAACCTTGCG GCCAACAACC TGGTAGAGAG CGGCCACCCA 
CGCAGTGGGG AGGTGAAACA ATATCAGGAC CGCCTAAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCGGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAAACCAG CAAGTGGATC GTGGACAAGA CGAAGGTTGT GGAGTCTACC 
AAGGACTTGG GGCAGGACCT AGCAGGAGTC ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGACG TATTGGCCAT CCGGGATCGT GTGAGCGCCT TGGAACGGGA GTCGCAGTAC 
TTGATGGAGT CACACCCTGA CCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTCGAG 
AAGATGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGTGAGGCC 
AGCAAGCTGC AGGCTTTCCT ACAAGACCTG GATGACTTCA AGGCCTGGCT GTCGATGGCC 
CAAAAGGCAG TGGCCTCTGA GGACATGCCT GAGTCACTCC CAGAGGCTGA GCAACTCCTA 
CAGCAGCATG CAGCCATTAA AGAGGAGATT GATGCTCACA AAGACGATTA CCACCGGGTC 
AAGGCCTCTG GGGAAAAAGT GATCGAAGGT CAAACTGACC CAGATTACCA GCTTCTGGGC 
CAGCGACTGG AGGGTCTAGA TACTGACTGG GATGCTCTGT GGCGGATGTG GGAGAGCCGA 
GGGAACACCC TCACCCAGTG CCTGGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACC TGGAGCCCCC AGACTCCCTA 
GCAGCTGCAG AGGCCGGCAT CCGAAAGTTT GAGGATTTCT TAGTGTCTAT GGAAAACAAC 
CGAGATAAAG TCCTGAGTCC TGTGGACTCC GGAAACATGC TGGTGTCTGA GGGCAACCTG 
TACTCGAACA AGATCAAAGA GAAGGTGCAG CTGATTGAAG ACAGGCACAT GAAGAACAAT 
GAGAAAGCCC AGGAGGTTAC AGCTCTACTG AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACATC 
TCCTATGACG AAGCCCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC ATTTATGGCA 
GAGCTGGCTT CACACCAAGG GTGGCTTGAG AACATCGTCG CGGAGGGAAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAC GGATGTGGTG TCAGAAAGGC TGGAAGCCCT GCACAAGCTC 
TGGGATGAGC TGCAGACCAC CACAAAGGCG AAGACGGAGC AACTCTCTGC TGCCCGGAGC 
TCTGACCTGC GCTTGCAAAC CCACGCCGAC CTTAGCAAAT GGATCAGCGC CATGGAGGAC 
CAGCTGCGAT CCGACGACCT GGGCAAGGAT CTGACCACTG TCAACCGGAT GTTGGTTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCA 
GAGGCACCCC CACTGGGAGC AGAGGCAGGA GACACGGACA TGAGCATTGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTATC CAAAGCCAAG 
CTACAGATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCGTTG 
GCGCAGTCAG CTGACTATGG CACTAATCTG CAAACAGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT CCTAGGCCAC GCACCACGGG TGGAGGATGT GCTACATCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCTTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCGGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGAAGCTC ACGAGGCACA GCAGTACTAC CTGGATGCGG GCGAGGCTGA AGCGTGGATC 
AGCGAGCAGG AGCTCTACGT GTTCTCTGAC GAACCCCCTA AGGACGAAGA GGGCGCCATC 
GTGATGCTCA AGCGGCACCT TCGGCAGCAG CGCACGGTGG AGGAATATGG AAGGAATATC 
AAACAGCTGG CTGGCCGCGC CCAGAGCCTG CTGTCTGCAG GCCACCCGGA GGGGGAGCAG 
ATCATCAGAC TTCAGGGGCA AGTGGATAAG CAGTATGCCG GGCTGAAGGA CATGGCTGAG 
GAACGGAGGC GGAAGCTAGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTGGAGC AGTGGATTAC GGAGAAAGAG ATGGTGGCCT CATCCCAAGA AATGGGGCAG 
GACTTTGACC ACGTGACTAT GCTCCGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCTATCGGGC AGGAGCGAGT AGACAATGTG AATTCTATCA TTGAGCGTCT CATCGATGCG 
GGCCACAGCG AGGCAGCCAC CATTGCAGAG TGGAAGGATG GGCTGAATGA CATGTGGGCA 
GATCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACGGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGTTG 
CCAGAGGACG TGGGGCTGGA CGCCAGCACA GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AGCTCCACCT CCTGGGTGTG CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC TGGGGAGAAG GCAGATGCTA TCCAGAGCAA GGAGCAGGAG 
GTGTCCGCGG CCTGGCAGGC CCTGCTGGAC GCGTGTGCTG GGCGCCGAGC TCAGCTGGTA 
GATACGGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTCTCCTC GGTGGAACTG 
CTCTTGAAGT ATCACCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AACTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCTTC AGACGAGATC 
CGGGAGAAAT TACAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAAGCC 
CGCTCTGACA GGCTCCACAT GCTGCTGGAG GTGTGCCAGT TCTCGAGGGA CGCTTCTGTG 
GCCGAGGCAT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACG 
GTGGACAGCG TGGAAAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGACA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCTCCCC AGGTCCACCA TGCGGCCACG GAGAGAACAT CCCCGGTCAG TTTTATGTCC 
CGCTTGTCTA GCTCCTGGGA GTCACTGCTG CCAGAGCCTG CTCACCCCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptb ( XM_006240244.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240306.1 Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X1, mRNA.

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CloneID	ORa29427
Clone ID Related Accession (Same CDS sequence)	XM_006240244.3 , XM_017594143.1 , XM_017594142.1
Accession Version	XM_006240244.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, erythrocytic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240244.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCAG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATACAG CCGGATTAAT 
GCCCGCTGGG ACGCCCCAGA TGATGAACTG GACAACGATA GCAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTTCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA TCTCTACAAG 
GACCTGCGGG ATGGACGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCCGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAGCAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATCGTGGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACGATTA TCCTCCGATT CCAGATTCAA 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTT 
CTGTGGTGCC AGATGAAGAC AGCCGGCTAC CCCCAAGTCA ACGTAACCAA CTTCACCTCC 
AGCTGGAAGG ACGGCCTGGC CTTCAATGCC CTAATTCATA AACACCGGCC TGACCTCATT 
GACTTTGACA AGCTAAAGGA CTCCAATGCC CGGCATAACT TGGAGCACGC ATTTGATGTG 
GCCGAGCGTC AGCTGGGCAT CATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCAGACGAGA AGTCCATCAT CACCTACGTG GTAGCATTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTAG CGGTGGAGGG CAAGAGAGTC GGCAAGGTCA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACCTG GATTGAGCAA 
ACCATTAGCG TCCTGAACAG CCGCAAGTTT GCCAACTCAT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGCACTGTG GAGAAGCCAC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TCCTCCTGTT CACTATCCAA TCCCGAATGA GAGCCAACAA TCAGAAAGTG 
TACACGCCCC ACGATGGGAA GCTAGTGTCT GACATCAACA GGGCCTGGGA GAGCCTAGAG 
GAAGCAGAAT ACCAAAGGGA GCTGGCCCTG AGGAGCGAGC TCATTCGGCA GGAGAAACTG 
GAGCAGCTGG CGCGGCGCTT CGACCGTAAG GCAGCCATGA GAGAGACGTG GCTCAACGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTC GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACGGCTGCCT ATGAGGAGCG GGTGAAGGCA 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGCATCAGT 
GCCAGGAAGA ACAATATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGCGGC TCGAGGCCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGT 
ATCGACTGGA TGGATGAGAT CAAGGCGCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT ACAGAAACAC AAGCTAATGG AAGCCGACAT TGCTATCCAA 
GGGGACAAAG TGAAGACCAT CACCGCGGCC ACCCTGCAGT TCACTGAGGG GAAAGGGTAT 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA ATGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA CGAGGCCGAA AGCTGGATCA AGGAAAAGGA ACAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACG AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTCGAGG ACGAGCTCCG TGGGCTCGAT GCCCATCTGA AGCAGATCTT CCAGGAGGCT 
GAGGACATGG TTGCACAAAA GCAGTTTGGA CATCCACAGA TCGAGACCCG TGTCAAAGAA 
GTATCGGCTC AGTGGGACCA CTTGAAAGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTTTC CGGTGAAGAT GTGGGGCAAG ACGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTTCTAGAG GAGCTGGAGG AGAGCCGCGG GGTGATGGAG 
CACTTAGAGA ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTGACA 
AACCGTCTAC AGGCACTCAG GAAGCTCTAC CAGCAGGTGA TGGCCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACTGTGT TTGGAGAGTC AGACGCCTGT 
GAGCTGTGGA TGACTGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTTGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGCGT GAACCTTGCG GCCAACAACC TGGTAGAGAG CGGCCACCCA 
CGCAGTGGGG AGGTGAAACA ATATCAGGAC CGCCTAAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCGGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAAACCAG CAAGTGGATC GTGGACAAGA CGAAGGTTGT GGAGTCTACC 
AAGGACTTGG GGCAGGACCT AGCAGGAGTC ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGACG TATTGGCCAT CCGGGATCGT GTGAGCGCCT TGGAACGGGA GTCGCAGTAC 
TTGATGGAGT CACACCCTGA CCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTCGAG 
AAGATGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGTGAGGCC 
AGCAAGCTGC AGGCTTTCCT ACAAGACCTG GATGACTTCA AGGCCTGGCT GTCGATGGCC 
CAAAAGGCAG TGGCCTCTGA GGACATGCCT GAGTCACTCC CAGAGGCTGA GCAACTCCTA 
CAGCAGCATG CAGCCATTAA AGAGGAGATT GATGCTCACA AAGACGATTA CCACCGGGTC 
AAGGCCTCTG GGGAAAAAGT GATCGAAGGT CAAACTGACC CAGATTACCA GCTTCTGGGC 
CAGCGACTGG AGGGTCTAGA TACTGACTGG GATGCTCTGT GGCGGATGTG GGAGAGCCGA 
GGGAACACCC TCACCCAGTG CCTGGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACC TGGAGCCCCC AGACTCCCTA 
GCAGCTGCAG AGGCCGGCAT CCGAAAGTTT GAGGATTTCT TAGTGTCTAT GGAAAACAAC 
CGAGATAAAG TCCTGAGTCC TGTGGACTCC GGAAACATGC TGGTGTCTGA GGGCAACCTG 
TACTCGAACA AGATCAAAGA GAAGGTGCAG CTGATTGAAG ACAGGCACAT GAAGAACAAT 
GAGAAAGCCC AGGAGGTTAC AGCTCTACTG AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACATC 
TCCTATGACG AAGCCCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC ATTTATGGCA 
GAGCTGGCTT CACACCAAGG GTGGCTTGAG AACATCGTCG CGGAGGGAAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAC GGATGTGGTG TCAGAAAGGC TGGAAGCCCT GCACAAGCTC 
TGGGATGAGC TGCAGACCAC CACAAAGGCG AAGACGGAGC AACTCTCTGC TGCCCGGAGC 
TCTGACCTGC GCTTGCAAAC CCACGCCGAC CTTAGCAAAT GGATCAGCGC CATGGAGGAC 
CAGCTGCGAT CCGACGACCT GGGCAAGGAT CTGACCACTG TCAACCGGAT GTTGGTTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCA 
GAGGCACCCC CACTGGGAGC AGAGGCAGGA GACACGGACA TGAGCATTGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTATC CAAAGCCAAG 
CTACAGATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCGTTG 
GCGCAGTCAG CTGACTATGG CACTAATCTG CAAACAGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT CCTAGGCCAC GCACCACGGG TGGAGGATGT GCTACATCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCTTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCGGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGAAGCTC ACGAGGCACA GCAGTACTAC CTGGATGCGG GCGAGGCTGA AGCGTGGATC 
AGCGAGCAGG AGCTCTACGT GTTCTCTGAC GAACCCCCTA AGGACGAAGA GGGCGCCATC 
GTGATGCTCA AGCGGCACCT TCGGCAGCAG CGCACGGTGG AGGAATATGG AAGGAATATC 
AAACAGCTGG CTGGCCGCGC CCAGAGCCTG CTGTCTGCAG GCCACCCGGA GGGGGAGCAG 
ATCATCAGAC TTCAGGGGCA AGTGGATAAG CAGTATGCCG GGCTGAAGGA CATGGCTGAG 
GAACGGAGGC GGAAGCTAGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTGGAGC AGTGGATTAC GGAGAAAGAG ATGGTGGCCT CATCCCAAGA AATGGGGCAG 
GACTTTGACC ACGTGACTAT GCTCCGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCTATCGGGC AGGAGCGAGT AGACAATGTG AATTCTATCA TTGAGCGTCT CATCGATGCG 
GGCCACAGCG AGGCAGCCAC CATTGCAGAG TGGAAGGATG GGCTGAATGA CATGTGGGCA 
GATCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACGGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGTTG 
CCAGAGGACG TGGGGCTGGA CGCCAGCACA GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AGCTCCACCT CCTGGGTGTG CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC TGGGGAGAAG GCAGATGCTA TCCAGAGCAA GGAGCAGGAG 
GTGTCCGCGG CCTGGCAGGC CCTGCTGGAC GCGTGTGCTG GGCGCCGAGC TCAGCTGGTA 
GATACGGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTCTCCTC GGTGGAACTG 
CTCTTGAAGT ATCACCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AACTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCTTC AGACGAGATC 
CGGGAGAAAT TACAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAAGCC 
CGCTCTGACA GGCTCCACAT GCTGCTGGAG GTGTGCCAGT TCTCGAGGGA CGCTTCTGTG 
GCCGAGGCAT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACG 
GTGGACAGCG TGGAAAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGACA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCTCCCC AGGTCCACCA TGCGGCCACG GAGAGAACAT CCCCGGGGGA AGAAAGAGGG 
CCATGGCCTC AGGACCTGCA ACCACCACCC CTGCCAGGAC ACCACAAGGA TGAGCAGGAG 
AAATCCGTTG GGGATGAGAG GCCTGCCACA GAGCCCCTCT TTAAGGTCCT GGACACACCC 
CTGAGTGAGG GTGACGAACC CACAACGCTG CCAGCCCAGC GAGACCTCGG GCATACTGTG 
CAGATGGAGG GCTACTTGGG CAGAAAGCAC GACCTAGAGG GGCCCAACAA GAAGGCCTCT 
AACAGGTCCT GGAACAACCT GTACTGTGTG CTCAGGAACA GCCAGCTGAC CTTCTACAAG 
GACGCTAAGA ACCTGGCCCT CGGGGTGCCC TACCATGGAG AGGAGCCCCT GGCCCTGAGG 
CACGCCATCT GTGAGATTGC TGTCAACTAT AAGAAGAAGA AGCATGTGTT TAAGCTGAGG 
TTAAGCAACG GCAGTGAATG GCTTTTCCAT GGCAAGGATG AGGAGGAGAT GCTGTTGTGG 
CTACAGGGCA TGAGCACGGC CATCAACGAG TCCCAGAGCA TCCGTGTCAA GGCACAGAGT 
CTGCCCCTGC CTTCCCTGGC TGGTCCTGAT GCCAGCGTAG GCAAGAAGGA CAAGGAGAAG 
AGGTTTAGCT TCTTCCCCAA AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240306.1
CDS	267..7256
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDSSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRISDLYKDLRDGRMLIKLLEVLSGEMLPRPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPQVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFDVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTISVLNSRKFANSLSGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYQRELALRSELIRQEKLEQLARRFDRKAAMRETWLNENQRLVTQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVKALEDLAQELEKENYHDQKRISARKNNILRLWSYLQELLRSRRQRLEATLALQKLFQDMLHSIDWMDEIKAHILSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKTITAATLQFTEGKGYQPCDPQVIQDRVSHLEQCFSELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLKQIFQEAEDMVAQKQFGHPQIETRVKEVSAQWDHLKELAAFRKKDLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKEFLEELEESRGVMEHLENQAQGFPEEFRDSPDVTNRLQALRKLYQQVMAQAELRGHKLQEALDLYTVFGESDACELWMTEKGKWLDQMDIPNTLEDLEVVQHRFDILDQEMKTLMAQIDGVNLAANNLVESGHPRSGEVKQYQDRLNKRWQAFQAVVSEQREAVDSALRVNNYCVDCEETSKWIVDKTKVVESTKDLGQDLAGVIAIQRKLSGLERDVLAIRDRVSALERESQYLMESHPDQKEDIGQRQADVEKMWKGLQDALQGQELSLGEASKLQAFLQDLDDFKAWLSMAQKAVASEDMPESLPEAEQLLQQHAAIKEEIDAHKDDYHRVKASGEKVIEGQTDPDYQLLGQRLEGLDTDWDALWRMWESRGNTLTQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLAAAEAGIRKFEDFLVSMENNRDKVLSPVDSGNMLVSEGNLYSNKIKEKVQLIEDRHMKNNEKAQEVTALLKDNLELQNFLQNCKELTLWINDKLLTSPDISYDEARNLHNKWMKHQAFMAELASHQGWLENIVAEGRQLMAEKPQFTDVVSERLEALHKLWDELQTTTKAKTEQLSAARSSDLRLQTHADLSKWISAMEDQLRSDDLGKDLTTVNRMLVKLKRVEEQVNLRKEELEELFAEAPPLGAEAGDTDMSIEKRFLDLLEPLGRRKKQLELSKAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHAPRVEDVLHRGQELVKAAEIDCQDIEERLGHLQSSWDTLREAAAGRLQRLREAHEAQQYYLDAGEAEAWISEQELYVFSDEPPKDEEGAIVMLKRHLRQQRTVEEYGRNIKQLAGRAQSLLSAGHPEGEQIIRLQGQVDKQYAGLKDMAEERRRKLENMYHLFQLKREADDLEQWITEKEMVASSQEMGQDFDHVTMLRDKFRDFARETGAIGQERVDNVNSIIERLIDAGHSEAATIAEWKDGLNDMWADLLELIDTRMQLLAASYDLHRYFYTGTEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKADAIQSKEQEVSAAWQALLDACAGRRAQLVDTADKFRFFSMVRDLLSWMESIIRQIETQERPRDVSSVELLLKYHQGIKAEINTRAKNFSTCLELGESLLQRQHQASDEIREKLQQVISRRQEMNDKWEARSDRLHMLLEVCQFSRDASVAEAWLIAQEPYLASRDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQTPDRPTEEPGPQEEEGETAGEAPQVHHAATERTSPGEERGPWPQDLQPPPLPGHHKDEQEKSVGDERPATEPLFKVLDTPLSEGDEPTTLPAQRDLGHTVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSQLTFYKDAKNLALGVPYHGEEPLALRHAICEIAVNYKKKKHVFKLRLSNGSEWLFHGKDEEEMLLWLQGMSTAINESQSIRVKAQSLPLPSLAGPDASVGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	XM_006240244.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240244.3

ORF Insert Sequence:

ATGACATCAG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATACAG CCGGATTAAT 
GCCCGCTGGG ACGCCCCAGA TGATGAACTG GACAACGATA GCAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTTCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA TCTCTACAAG 
GACCTGCGGG ATGGACGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCCGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAGCAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATCGTGGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACGATTA TCCTCCGATT CCAGATTCAA 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTT 
CTGTGGTGCC AGATGAAGAC AGCCGGCTAC CCCCAAGTCA ACGTAACCAA CTTCACCTCC 
AGCTGGAAGG ACGGCCTGGC CTTCAATGCC CTAATTCATA AACACCGGCC TGACCTCATT 
GACTTTGACA AGCTAAAGGA CTCCAATGCC CGGCATAACT TGGAGCACGC ATTTGATGTG 
GCCGAGCGTC AGCTGGGCAT CATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCAGACGAGA AGTCCATCAT CACCTACGTG GTAGCATTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTAG CGGTGGAGGG CAAGAGAGTC GGCAAGGTCA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACCTG GATTGAGCAA 
ACCATTAGCG TCCTGAACAG CCGCAAGTTT GCCAACTCAT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGCACTGTG GAGAAGCCAC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TCCTCCTGTT CACTATCCAA TCCCGAATGA GAGCCAACAA TCAGAAAGTG 
TACACGCCCC ACGATGGGAA GCTAGTGTCT GACATCAACA GGGCCTGGGA GAGCCTAGAG 
GAAGCAGAAT ACCAAAGGGA GCTGGCCCTG AGGAGCGAGC TCATTCGGCA GGAGAAACTG 
GAGCAGCTGG CGCGGCGCTT CGACCGTAAG GCAGCCATGA GAGAGACGTG GCTCAACGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTC GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACGGCTGCCT ATGAGGAGCG GGTGAAGGCA 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGCATCAGT 
GCCAGGAAGA ACAATATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGCGGC TCGAGGCCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGT 
ATCGACTGGA TGGATGAGAT CAAGGCGCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT ACAGAAACAC AAGCTAATGG AAGCCGACAT TGCTATCCAA 
GGGGACAAAG TGAAGACCAT CACCGCGGCC ACCCTGCAGT TCACTGAGGG GAAAGGGTAT 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA ATGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA CGAGGCCGAA AGCTGGATCA AGGAAAAGGA ACAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACG AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTCGAGG ACGAGCTCCG TGGGCTCGAT GCCCATCTGA AGCAGATCTT CCAGGAGGCT 
GAGGACATGG TTGCACAAAA GCAGTTTGGA CATCCACAGA TCGAGACCCG TGTCAAAGAA 
GTATCGGCTC AGTGGGACCA CTTGAAAGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTTTC CGGTGAAGAT GTGGGGCAAG ACGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTTCTAGAG GAGCTGGAGG AGAGCCGCGG GGTGATGGAG 
CACTTAGAGA ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTGACA 
AACCGTCTAC AGGCACTCAG GAAGCTCTAC CAGCAGGTGA TGGCCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACTGTGT TTGGAGAGTC AGACGCCTGT 
GAGCTGTGGA TGACTGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTTGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGCGT GAACCTTGCG GCCAACAACC TGGTAGAGAG CGGCCACCCA 
CGCAGTGGGG AGGTGAAACA ATATCAGGAC CGCCTAAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCGGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAAACCAG CAAGTGGATC GTGGACAAGA CGAAGGTTGT GGAGTCTACC 
AAGGACTTGG GGCAGGACCT AGCAGGAGTC ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGACG TATTGGCCAT CCGGGATCGT GTGAGCGCCT TGGAACGGGA GTCGCAGTAC 
TTGATGGAGT CACACCCTGA CCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTCGAG 
AAGATGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGTGAGGCC 
AGCAAGCTGC AGGCTTTCCT ACAAGACCTG GATGACTTCA AGGCCTGGCT GTCGATGGCC 
CAAAAGGCAG TGGCCTCTGA GGACATGCCT GAGTCACTCC CAGAGGCTGA GCAACTCCTA 
CAGCAGCATG CAGCCATTAA AGAGGAGATT GATGCTCACA AAGACGATTA CCACCGGGTC 
AAGGCCTCTG GGGAAAAAGT GATCGAAGGT CAAACTGACC CAGATTACCA GCTTCTGGGC 
CAGCGACTGG AGGGTCTAGA TACTGACTGG GATGCTCTGT GGCGGATGTG GGAGAGCCGA 
GGGAACACCC TCACCCAGTG CCTGGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACC TGGAGCCCCC AGACTCCCTA 
GCAGCTGCAG AGGCCGGCAT CCGAAAGTTT GAGGATTTCT TAGTGTCTAT GGAAAACAAC 
CGAGATAAAG TCCTGAGTCC TGTGGACTCC GGAAACATGC TGGTGTCTGA GGGCAACCTG 
TACTCGAACA AGATCAAAGA GAAGGTGCAG CTGATTGAAG ACAGGCACAT GAAGAACAAT 
GAGAAAGCCC AGGAGGTTAC AGCTCTACTG AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACATC 
TCCTATGACG AAGCCCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC ATTTATGGCA 
GAGCTGGCTT CACACCAAGG GTGGCTTGAG AACATCGTCG CGGAGGGAAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAC GGATGTGGTG TCAGAAAGGC TGGAAGCCCT GCACAAGCTC 
TGGGATGAGC TGCAGACCAC CACAAAGGCG AAGACGGAGC AACTCTCTGC TGCCCGGAGC 
TCTGACCTGC GCTTGCAAAC CCACGCCGAC CTTAGCAAAT GGATCAGCGC CATGGAGGAC 
CAGCTGCGAT CCGACGACCT GGGCAAGGAT CTGACCACTG TCAACCGGAT GTTGGTTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCA 
GAGGCACCCC CACTGGGAGC AGAGGCAGGA GACACGGACA TGAGCATTGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTATC CAAAGCCAAG 
CTACAGATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCGTTG 
GCGCAGTCAG CTGACTATGG CACTAATCTG CAAACAGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT CCTAGGCCAC GCACCACGGG TGGAGGATGT GCTACATCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCTTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCGGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGAAGCTC ACGAGGCACA GCAGTACTAC CTGGATGCGG GCGAGGCTGA AGCGTGGATC 
AGCGAGCAGG AGCTCTACGT GTTCTCTGAC GAACCCCCTA AGGACGAAGA GGGCGCCATC 
GTGATGCTCA AGCGGCACCT TCGGCAGCAG CGCACGGTGG AGGAATATGG AAGGAATATC 
AAACAGCTGG CTGGCCGCGC CCAGAGCCTG CTGTCTGCAG GCCACCCGGA GGGGGAGCAG 
ATCATCAGAC TTCAGGGGCA AGTGGATAAG CAGTATGCCG GGCTGAAGGA CATGGCTGAG 
GAACGGAGGC GGAAGCTAGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTGGAGC AGTGGATTAC GGAGAAAGAG ATGGTGGCCT CATCCCAAGA AATGGGGCAG 
GACTTTGACC ACGTGACTAT GCTCCGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCTATCGGGC AGGAGCGAGT AGACAATGTG AATTCTATCA TTGAGCGTCT CATCGATGCG 
GGCCACAGCG AGGCAGCCAC CATTGCAGAG TGGAAGGATG GGCTGAATGA CATGTGGGCA 
GATCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACGGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGTTG 
CCAGAGGACG TGGGGCTGGA CGCCAGCACA GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AGCTCCACCT CCTGGGTGTG CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC TGGGGAGAAG GCAGATGCTA TCCAGAGCAA GGAGCAGGAG 
GTGTCCGCGG CCTGGCAGGC CCTGCTGGAC GCGTGTGCTG GGCGCCGAGC TCAGCTGGTA 
GATACGGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTCTCCTC GGTGGAACTG 
CTCTTGAAGT ATCACCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AACTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCTTC AGACGAGATC 
CGGGAGAAAT TACAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAAGCC 
CGCTCTGACA GGCTCCACAT GCTGCTGGAG GTGTGCCAGT TCTCGAGGGA CGCTTCTGTG 
GCCGAGGCAT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACG 
GTGGACAGCG TGGAAAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGACA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCTCCCC AGGTCCACCA TGCGGCCACG GAGAGAACAT CCCCGGGGGA AGAAAGAGGG 
CCATGGCCTC AGGACCTGCA ACCACCACCC CTGCCAGGAC ACCACAAGGA TGAGCAGGAG 
AAATCCGTTG GGGATGAGAG GCCTGCCACA GAGCCCCTCT TTAAGGTCCT GGACACACCC 
CTGAGTGAGG GTGACGAACC CACAACGCTG CCAGCCCAGC GAGACCTCGG GCATACTGTG 
CAGATGGAGG GCTACTTGGG CAGAAAGCAC GACCTAGAGG GGCCCAACAA GAAGGCCTCT 
AACAGGTCCT GGAACAACCT GTACTGTGTG CTCAGGAACA GCCAGCTGAC CTTCTACAAG 
GACGCTAAGA ACCTGGCCCT CGGGGTGCCC TACCATGGAG AGGAGCCCCT GGCCCTGAGG 
CACGCCATCT GTGAGATTGC TGTCAACTAT AAGAAGAAGA AGCATGTGTT TAAGCTGAGG 
TTAAGCAACG GCAGTGAATG GCTTTTCCAT GGCAAGGATG AGGAGGAGAT GCTGTTGTGG 
CTACAGGGCA TGAGCACGGC CATCAACGAG TCCCAGAGCA TCCGTGTCAA GGCACAGAGT 
CTGCCCCTGC CTTCCCTGGC TGGTCCTGAT GCCAGCGTAG GCAAGAAGGA CAAGGAGAAG 
AGGTTTAGCT TCTTCCCCAA AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptb ( XM_017594143.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449632.1 Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X3, mRNA.

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CloneID	ORa29427
Clone ID Related Accession (Same CDS sequence)	XM_006240244.3 , XM_017594143.1 , XM_017594142.1
Accession Version	XM_017594143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, erythrocytic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCAG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATACAG CCGGATTAAT 
GCCCGCTGGG ACGCCCCAGA TGATGAACTG GACAACGATA GCAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTTCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA TCTCTACAAG 
GACCTGCGGG ATGGACGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCCGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAGCAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATCGTGGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACGATTA TCCTCCGATT CCAGATTCAA 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTT 
CTGTGGTGCC AGATGAAGAC AGCCGGCTAC CCCCAAGTCA ACGTAACCAA CTTCACCTCC 
AGCTGGAAGG ACGGCCTGGC CTTCAATGCC CTAATTCATA AACACCGGCC TGACCTCATT 
GACTTTGACA AGCTAAAGGA CTCCAATGCC CGGCATAACT TGGAGCACGC ATTTGATGTG 
GCCGAGCGTC AGCTGGGCAT CATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCAGACGAGA AGTCCATCAT CACCTACGTG GTAGCATTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTAG CGGTGGAGGG CAAGAGAGTC GGCAAGGTCA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACCTG GATTGAGCAA 
ACCATTAGCG TCCTGAACAG CCGCAAGTTT GCCAACTCAT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGCACTGTG GAGAAGCCAC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TCCTCCTGTT CACTATCCAA TCCCGAATGA GAGCCAACAA TCAGAAAGTG 
TACACGCCCC ACGATGGGAA GCTAGTGTCT GACATCAACA GGGCCTGGGA GAGCCTAGAG 
GAAGCAGAAT ACCAAAGGGA GCTGGCCCTG AGGAGCGAGC TCATTCGGCA GGAGAAACTG 
GAGCAGCTGG CGCGGCGCTT CGACCGTAAG GCAGCCATGA GAGAGACGTG GCTCAACGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTC GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACGGCTGCCT ATGAGGAGCG GGTGAAGGCA 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGCATCAGT 
GCCAGGAAGA ACAATATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGCGGC TCGAGGCCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGT 
ATCGACTGGA TGGATGAGAT CAAGGCGCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT ACAGAAACAC AAGCTAATGG AAGCCGACAT TGCTATCCAA 
GGGGACAAAG TGAAGACCAT CACCGCGGCC ACCCTGCAGT TCACTGAGGG GAAAGGGTAT 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA ATGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA CGAGGCCGAA AGCTGGATCA AGGAAAAGGA ACAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACG AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTCGAGG ACGAGCTCCG TGGGCTCGAT GCCCATCTGA AGCAGATCTT CCAGGAGGCT 
GAGGACATGG TTGCACAAAA GCAGTTTGGA CATCCACAGA TCGAGACCCG TGTCAAAGAA 
GTATCGGCTC AGTGGGACCA CTTGAAAGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTTTC CGGTGAAGAT GTGGGGCAAG ACGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTTCTAGAG GAGCTGGAGG AGAGCCGCGG GGTGATGGAG 
CACTTAGAGA ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTGACA 
AACCGTCTAC AGGCACTCAG GAAGCTCTAC CAGCAGGTGA TGGCCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACTGTGT TTGGAGAGTC AGACGCCTGT 
GAGCTGTGGA TGACTGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTTGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGCGT GAACCTTGCG GCCAACAACC TGGTAGAGAG CGGCCACCCA 
CGCAGTGGGG AGGTGAAACA ATATCAGGAC CGCCTAAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCGGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAAACCAG CAAGTGGATC GTGGACAAGA CGAAGGTTGT GGAGTCTACC 
AAGGACTTGG GGCAGGACCT AGCAGGAGTC ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGACG TATTGGCCAT CCGGGATCGT GTGAGCGCCT TGGAACGGGA GTCGCAGTAC 
TTGATGGAGT CACACCCTGA CCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTCGAG 
AAGATGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGTGAGGCC 
AGCAAGCTGC AGGCTTTCCT ACAAGACCTG GATGACTTCA AGGCCTGGCT GTCGATGGCC 
CAAAAGGCAG TGGCCTCTGA GGACATGCCT GAGTCACTCC CAGAGGCTGA GCAACTCCTA 
CAGCAGCATG CAGCCATTAA AGAGGAGATT GATGCTCACA AAGACGATTA CCACCGGGTC 
AAGGCCTCTG GGGAAAAAGT GATCGAAGGT CAAACTGACC CAGATTACCA GCTTCTGGGC 
CAGCGACTGG AGGGTCTAGA TACTGACTGG GATGCTCTGT GGCGGATGTG GGAGAGCCGA 
GGGAACACCC TCACCCAGTG CCTGGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACC TGGAGCCCCC AGACTCCCTA 
GCAGCTGCAG AGGCCGGCAT CCGAAAGTTT GAGGATTTCT TAGTGTCTAT GGAAAACAAC 
CGAGATAAAG TCCTGAGTCC TGTGGACTCC GGAAACATGC TGGTGTCTGA GGGCAACCTG 
TACTCGAACA AGATCAAAGA GAAGGTGCAG CTGATTGAAG ACAGGCACAT GAAGAACAAT 
GAGAAAGCCC AGGAGGTTAC AGCTCTACTG AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACATC 
TCCTATGACG AAGCCCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC ATTTATGGCA 
GAGCTGGCTT CACACCAAGG GTGGCTTGAG AACATCGTCG CGGAGGGAAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAC GGATGTGGTG TCAGAAAGGC TGGAAGCCCT GCACAAGCTC 
TGGGATGAGC TGCAGACCAC CACAAAGGCG AAGACGGAGC AACTCTCTGC TGCCCGGAGC 
TCTGACCTGC GCTTGCAAAC CCACGCCGAC CTTAGCAAAT GGATCAGCGC CATGGAGGAC 
CAGCTGCGAT CCGACGACCT GGGCAAGGAT CTGACCACTG TCAACCGGAT GTTGGTTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCA 
GAGGCACCCC CACTGGGAGC AGAGGCAGGA GACACGGACA TGAGCATTGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTATC CAAAGCCAAG 
CTACAGATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCGTTG 
GCGCAGTCAG CTGACTATGG CACTAATCTG CAAACAGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT CCTAGGCCAC GCACCACGGG TGGAGGATGT GCTACATCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCTTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCGGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGAAGCTC ACGAGGCACA GCAGTACTAC CTGGATGCGG GCGAGGCTGA AGCGTGGATC 
AGCGAGCAGG AGCTCTACGT GTTCTCTGAC GAACCCCCTA AGGACGAAGA GGGCGCCATC 
GTGATGCTCA AGCGGCACCT TCGGCAGCAG CGCACGGTGG AGGAATATGG AAGGAATATC 
AAACAGCTGG CTGGCCGCGC CCAGAGCCTG CTGTCTGCAG GCCACCCGGA GGGGGAGCAG 
ATCATCAGAC TTCAGGGGCA AGTGGATAAG CAGTATGCCG GGCTGAAGGA CATGGCTGAG 
GAACGGAGGC GGAAGCTAGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTGGAGC AGTGGATTAC GGAGAAAGAG ATGGTGGCCT CATCCCAAGA AATGGGGCAG 
GACTTTGACC ACGTGACTAT GCTCCGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCTATCGGGC AGGAGCGAGT AGACAATGTG AATTCTATCA TTGAGCGTCT CATCGATGCG 
GGCCACAGCG AGGCAGCCAC CATTGCAGAG TGGAAGGATG GGCTGAATGA CATGTGGGCA 
GATCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACGGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGTTG 
CCAGAGGACG TGGGGCTGGA CGCCAGCACA GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AGCTCCACCT CCTGGGTGTG CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC TGGGGAGAAG GCAGATGCTA TCCAGAGCAA GGAGCAGGAG 
GTGTCCGCGG CCTGGCAGGC CCTGCTGGAC GCGTGTGCTG GGCGCCGAGC TCAGCTGGTA 
GATACGGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTCTCCTC GGTGGAACTG 
CTCTTGAAGT ATCACCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AACTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCTTC AGACGAGATC 
CGGGAGAAAT TACAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAAGCC 
CGCTCTGACA GGCTCCACAT GCTGCTGGAG GTGTGCCAGT TCTCGAGGGA CGCTTCTGTG 
GCCGAGGCAT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACG 
GTGGACAGCG TGGAAAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGACA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCTCCCC AGGTCCACCA TGCGGCCACG GAGAGAACAT CCCCGGGGGA AGAAAGAGGG 
CCATGGCCTC AGGACCTGCA ACCACCACCC CTGCCAGGAC ACCACAAGGA TGAGCAGGAG 
AAATCCGTTG GGGATGAGAG GCCTGCCACA GAGCCCCTCT TTAAGGTCCT GGACACACCC 
CTGAGTGAGG GTGACGAACC CACAACGCTG CCAGCCCAGC GAGACCTCGG GCATACTGTG 
CAGATGGAGG GCTACTTGGG CAGAAAGCAC GACCTAGAGG GGCCCAACAA GAAGGCCTCT 
AACAGGTCCT GGAACAACCT GTACTGTGTG CTCAGGAACA GCCAGCTGAC CTTCTACAAG 
GACGCTAAGA ACCTGGCCCT CGGGGTGCCC TACCATGGAG AGGAGCCCCT GGCCCTGAGG 
CACGCCATCT GTGAGATTGC TGTCAACTAT AAGAAGAAGA AGCATGTGTT TAAGCTGAGG 
TTAAGCAACG GCAGTGAATG GCTTTTCCAT GGCAAGGATG AGGAGGAGAT GCTGTTGTGG 
CTACAGGGCA TGAGCACGGC CATCAACGAG TCCCAGAGCA TCCGTGTCAA GGCACAGAGT 
CTGCCCCTGC CTTCCCTGGC TGGTCCTGAT GCCAGCGTAG GCAAGAAGGA CAAGGAGAAG 
AGGTTTAGCT TCTTCCCCAA AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449632.1
CDS	361..7350
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDSSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRISDLYKDLRDGRMLIKLLEVLSGEMLPRPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPQVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFDVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTISVLNSRKFANSLSGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYQRELALRSELIRQEKLEQLARRFDRKAAMRETWLNENQRLVTQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVKALEDLAQELEKENYHDQKRISARKNNILRLWSYLQELLRSRRQRLEATLALQKLFQDMLHSIDWMDEIKAHILSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKTITAATLQFTEGKGYQPCDPQVIQDRVSHLEQCFSELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLKQIFQEAEDMVAQKQFGHPQIETRVKEVSAQWDHLKELAAFRKKDLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKEFLEELEESRGVMEHLENQAQGFPEEFRDSPDVTNRLQALRKLYQQVMAQAELRGHKLQEALDLYTVFGESDACELWMTEKGKWLDQMDIPNTLEDLEVVQHRFDILDQEMKTLMAQIDGVNLAANNLVESGHPRSGEVKQYQDRLNKRWQAFQAVVSEQREAVDSALRVNNYCVDCEETSKWIVDKTKVVESTKDLGQDLAGVIAIQRKLSGLERDVLAIRDRVSALERESQYLMESHPDQKEDIGQRQADVEKMWKGLQDALQGQELSLGEASKLQAFLQDLDDFKAWLSMAQKAVASEDMPESLPEAEQLLQQHAAIKEEIDAHKDDYHRVKASGEKVIEGQTDPDYQLLGQRLEGLDTDWDALWRMWESRGNTLTQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLAAAEAGIRKFEDFLVSMENNRDKVLSPVDSGNMLVSEGNLYSNKIKEKVQLIEDRHMKNNEKAQEVTALLKDNLELQNFLQNCKELTLWINDKLLTSPDISYDEARNLHNKWMKHQAFMAELASHQGWLENIVAEGRQLMAEKPQFTDVVSERLEALHKLWDELQTTTKAKTEQLSAARSSDLRLQTHADLSKWISAMEDQLRSDDLGKDLTTVNRMLVKLKRVEEQVNLRKEELEELFAEAPPLGAEAGDTDMSIEKRFLDLLEPLGRRKKQLELSKAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHAPRVEDVLHRGQELVKAAEIDCQDIEERLGHLQSSWDTLREAAAGRLQRLREAHEAQQYYLDAGEAEAWISEQELYVFSDEPPKDEEGAIVMLKRHLRQQRTVEEYGRNIKQLAGRAQSLLSAGHPEGEQIIRLQGQVDKQYAGLKDMAEERRRKLENMYHLFQLKREADDLEQWITEKEMVASSQEMGQDFDHVTMLRDKFRDFARETGAIGQERVDNVNSIIERLIDAGHSEAATIAEWKDGLNDMWADLLELIDTRMQLLAASYDLHRYFYTGTEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKADAIQSKEQEVSAAWQALLDACAGRRAQLVDTADKFRFFSMVRDLLSWMESIIRQIETQERPRDVSSVELLLKYHQGIKAEINTRAKNFSTCLELGESLLQRQHQASDEIREKLQQVISRRQEMNDKWEARSDRLHMLLEVCQFSRDASVAEAWLIAQEPYLASRDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQTPDRPTEEPGPQEEEGETAGEAPQVHHAATERTSPGEERGPWPQDLQPPPLPGHHKDEQEKSVGDERPATEPLFKVLDTPLSEGDEPTTLPAQRDLGHTVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSQLTFYKDAKNLALGVPYHGEEPLALRHAICEIAVNYKKKKHVFKLRLSNGSEWLFHGKDEEEMLLWLQGMSTAINESQSIRVKAQSLPLPSLAGPDASVGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	XM_017594143.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594143.1

ORF Insert Sequence:

ATGACATCAG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATACAG CCGGATTAAT 
GCCCGCTGGG ACGCCCCAGA TGATGAACTG GACAACGATA GCAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTTCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA TCTCTACAAG 
GACCTGCGGG ATGGACGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCCGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAGCAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATCGTGGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACGATTA TCCTCCGATT CCAGATTCAA 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTT 
CTGTGGTGCC AGATGAAGAC AGCCGGCTAC CCCCAAGTCA ACGTAACCAA CTTCACCTCC 
AGCTGGAAGG ACGGCCTGGC CTTCAATGCC CTAATTCATA AACACCGGCC TGACCTCATT 
GACTTTGACA AGCTAAAGGA CTCCAATGCC CGGCATAACT TGGAGCACGC ATTTGATGTG 
GCCGAGCGTC AGCTGGGCAT CATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCAGACGAGA AGTCCATCAT CACCTACGTG GTAGCATTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTAG CGGTGGAGGG CAAGAGAGTC GGCAAGGTCA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACCTG GATTGAGCAA 
ACCATTAGCG TCCTGAACAG CCGCAAGTTT GCCAACTCAT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGCACTGTG GAGAAGCCAC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TCCTCCTGTT CACTATCCAA TCCCGAATGA GAGCCAACAA TCAGAAAGTG 
TACACGCCCC ACGATGGGAA GCTAGTGTCT GACATCAACA GGGCCTGGGA GAGCCTAGAG 
GAAGCAGAAT ACCAAAGGGA GCTGGCCCTG AGGAGCGAGC TCATTCGGCA GGAGAAACTG 
GAGCAGCTGG CGCGGCGCTT CGACCGTAAG GCAGCCATGA GAGAGACGTG GCTCAACGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTC GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACGGCTGCCT ATGAGGAGCG GGTGAAGGCA 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGCATCAGT 
GCCAGGAAGA ACAATATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGCGGC TCGAGGCCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGT 
ATCGACTGGA TGGATGAGAT CAAGGCGCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT ACAGAAACAC AAGCTAATGG AAGCCGACAT TGCTATCCAA 
GGGGACAAAG TGAAGACCAT CACCGCGGCC ACCCTGCAGT TCACTGAGGG GAAAGGGTAT 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA ATGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA CGAGGCCGAA AGCTGGATCA AGGAAAAGGA ACAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACG AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTCGAGG ACGAGCTCCG TGGGCTCGAT GCCCATCTGA AGCAGATCTT CCAGGAGGCT 
GAGGACATGG TTGCACAAAA GCAGTTTGGA CATCCACAGA TCGAGACCCG TGTCAAAGAA 
GTATCGGCTC AGTGGGACCA CTTGAAAGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTTTC CGGTGAAGAT GTGGGGCAAG ACGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTTCTAGAG GAGCTGGAGG AGAGCCGCGG GGTGATGGAG 
CACTTAGAGA ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTGACA 
AACCGTCTAC AGGCACTCAG GAAGCTCTAC CAGCAGGTGA TGGCCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACTGTGT TTGGAGAGTC AGACGCCTGT 
GAGCTGTGGA TGACTGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTTGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGCGT GAACCTTGCG GCCAACAACC TGGTAGAGAG CGGCCACCCA 
CGCAGTGGGG AGGTGAAACA ATATCAGGAC CGCCTAAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCGGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAAACCAG CAAGTGGATC GTGGACAAGA CGAAGGTTGT GGAGTCTACC 
AAGGACTTGG GGCAGGACCT AGCAGGAGTC ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGACG TATTGGCCAT CCGGGATCGT GTGAGCGCCT TGGAACGGGA GTCGCAGTAC 
TTGATGGAGT CACACCCTGA CCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTCGAG 
AAGATGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGTGAGGCC 
AGCAAGCTGC AGGCTTTCCT ACAAGACCTG GATGACTTCA AGGCCTGGCT GTCGATGGCC 
CAAAAGGCAG TGGCCTCTGA GGACATGCCT GAGTCACTCC CAGAGGCTGA GCAACTCCTA 
CAGCAGCATG CAGCCATTAA AGAGGAGATT GATGCTCACA AAGACGATTA CCACCGGGTC 
AAGGCCTCTG GGGAAAAAGT GATCGAAGGT CAAACTGACC CAGATTACCA GCTTCTGGGC 
CAGCGACTGG AGGGTCTAGA TACTGACTGG GATGCTCTGT GGCGGATGTG GGAGAGCCGA 
GGGAACACCC TCACCCAGTG CCTGGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACC TGGAGCCCCC AGACTCCCTA 
GCAGCTGCAG AGGCCGGCAT CCGAAAGTTT GAGGATTTCT TAGTGTCTAT GGAAAACAAC 
CGAGATAAAG TCCTGAGTCC TGTGGACTCC GGAAACATGC TGGTGTCTGA GGGCAACCTG 
TACTCGAACA AGATCAAAGA GAAGGTGCAG CTGATTGAAG ACAGGCACAT GAAGAACAAT 
GAGAAAGCCC AGGAGGTTAC AGCTCTACTG AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACATC 
TCCTATGACG AAGCCCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC ATTTATGGCA 
GAGCTGGCTT CACACCAAGG GTGGCTTGAG AACATCGTCG CGGAGGGAAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAC GGATGTGGTG TCAGAAAGGC TGGAAGCCCT GCACAAGCTC 
TGGGATGAGC TGCAGACCAC CACAAAGGCG AAGACGGAGC AACTCTCTGC TGCCCGGAGC 
TCTGACCTGC GCTTGCAAAC CCACGCCGAC CTTAGCAAAT GGATCAGCGC CATGGAGGAC 
CAGCTGCGAT CCGACGACCT GGGCAAGGAT CTGACCACTG TCAACCGGAT GTTGGTTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCA 
GAGGCACCCC CACTGGGAGC AGAGGCAGGA GACACGGACA TGAGCATTGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTATC CAAAGCCAAG 
CTACAGATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCGTTG 
GCGCAGTCAG CTGACTATGG CACTAATCTG CAAACAGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT CCTAGGCCAC GCACCACGGG TGGAGGATGT GCTACATCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCTTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCGGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGAAGCTC ACGAGGCACA GCAGTACTAC CTGGATGCGG GCGAGGCTGA AGCGTGGATC 
AGCGAGCAGG AGCTCTACGT GTTCTCTGAC GAACCCCCTA AGGACGAAGA GGGCGCCATC 
GTGATGCTCA AGCGGCACCT TCGGCAGCAG CGCACGGTGG AGGAATATGG AAGGAATATC 
AAACAGCTGG CTGGCCGCGC CCAGAGCCTG CTGTCTGCAG GCCACCCGGA GGGGGAGCAG 
ATCATCAGAC TTCAGGGGCA AGTGGATAAG CAGTATGCCG GGCTGAAGGA CATGGCTGAG 
GAACGGAGGC GGAAGCTAGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTGGAGC AGTGGATTAC GGAGAAAGAG ATGGTGGCCT CATCCCAAGA AATGGGGCAG 
GACTTTGACC ACGTGACTAT GCTCCGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCTATCGGGC AGGAGCGAGT AGACAATGTG AATTCTATCA TTGAGCGTCT CATCGATGCG 
GGCCACAGCG AGGCAGCCAC CATTGCAGAG TGGAAGGATG GGCTGAATGA CATGTGGGCA 
GATCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACGGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGTTG 
CCAGAGGACG TGGGGCTGGA CGCCAGCACA GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AGCTCCACCT CCTGGGTGTG CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC TGGGGAGAAG GCAGATGCTA TCCAGAGCAA GGAGCAGGAG 
GTGTCCGCGG CCTGGCAGGC CCTGCTGGAC GCGTGTGCTG GGCGCCGAGC TCAGCTGGTA 
GATACGGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTCTCCTC GGTGGAACTG 
CTCTTGAAGT ATCACCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AACTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCTTC AGACGAGATC 
CGGGAGAAAT TACAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAAGCC 
CGCTCTGACA GGCTCCACAT GCTGCTGGAG GTGTGCCAGT TCTCGAGGGA CGCTTCTGTG 
GCCGAGGCAT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACG 
GTGGACAGCG TGGAAAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGACA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCTCCCC AGGTCCACCA TGCGGCCACG GAGAGAACAT CCCCGGGGGA AGAAAGAGGG 
CCATGGCCTC AGGACCTGCA ACCACCACCC CTGCCAGGAC ACCACAAGGA TGAGCAGGAG 
AAATCCGTTG GGGATGAGAG GCCTGCCACA GAGCCCCTCT TTAAGGTCCT GGACACACCC 
CTGAGTGAGG GTGACGAACC CACAACGCTG CCAGCCCAGC GAGACCTCGG GCATACTGTG 
CAGATGGAGG GCTACTTGGG CAGAAAGCAC GACCTAGAGG GGCCCAACAA GAAGGCCTCT 
AACAGGTCCT GGAACAACCT GTACTGTGTG CTCAGGAACA GCCAGCTGAC CTTCTACAAG 
GACGCTAAGA ACCTGGCCCT CGGGGTGCCC TACCATGGAG AGGAGCCCCT GGCCCTGAGG 
CACGCCATCT GTGAGATTGC TGTCAACTAT AAGAAGAAGA AGCATGTGTT TAAGCTGAGG 
TTAAGCAACG GCAGTGAATG GCTTTTCCAT GGCAAGGATG AGGAGGAGAT GCTGTTGTGG 
CTACAGGGCA TGAGCACGGC CATCAACGAG TCCCAGAGCA TCCGTGTCAA GGCACAGAGT 
CTGCCCCTGC CTTCCCTGGC TGGTCCTGAT GCCAGCGTAG GCAAGAAGGA CAAGGAGAAG 
AGGTTTAGCT TCTTCCCCAA AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptb ( XM_017594142.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449631.1 Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X2, mRNA.

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CloneID	ORa29427
Clone ID Related Accession (Same CDS sequence)	XM_006240244.3 , XM_017594143.1 , XM_017594142.1
Accession Version	XM_017594142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, erythrocytic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCAG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATACAG CCGGATTAAT 
GCCCGCTGGG ACGCCCCAGA TGATGAACTG GACAACGATA GCAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTTCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA TCTCTACAAG 
GACCTGCGGG ATGGACGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCCGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAGCAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATCGTGGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACGATTA TCCTCCGATT CCAGATTCAA 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTT 
CTGTGGTGCC AGATGAAGAC AGCCGGCTAC CCCCAAGTCA ACGTAACCAA CTTCACCTCC 
AGCTGGAAGG ACGGCCTGGC CTTCAATGCC CTAATTCATA AACACCGGCC TGACCTCATT 
GACTTTGACA AGCTAAAGGA CTCCAATGCC CGGCATAACT TGGAGCACGC ATTTGATGTG 
GCCGAGCGTC AGCTGGGCAT CATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCAGACGAGA AGTCCATCAT CACCTACGTG GTAGCATTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTAG CGGTGGAGGG CAAGAGAGTC GGCAAGGTCA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACCTG GATTGAGCAA 
ACCATTAGCG TCCTGAACAG CCGCAAGTTT GCCAACTCAT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGCACTGTG GAGAAGCCAC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TCCTCCTGTT CACTATCCAA TCCCGAATGA GAGCCAACAA TCAGAAAGTG 
TACACGCCCC ACGATGGGAA GCTAGTGTCT GACATCAACA GGGCCTGGGA GAGCCTAGAG 
GAAGCAGAAT ACCAAAGGGA GCTGGCCCTG AGGAGCGAGC TCATTCGGCA GGAGAAACTG 
GAGCAGCTGG CGCGGCGCTT CGACCGTAAG GCAGCCATGA GAGAGACGTG GCTCAACGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTC GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACGGCTGCCT ATGAGGAGCG GGTGAAGGCA 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGCATCAGT 
GCCAGGAAGA ACAATATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGCGGC TCGAGGCCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGT 
ATCGACTGGA TGGATGAGAT CAAGGCGCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT ACAGAAACAC AAGCTAATGG AAGCCGACAT TGCTATCCAA 
GGGGACAAAG TGAAGACCAT CACCGCGGCC ACCCTGCAGT TCACTGAGGG GAAAGGGTAT 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA ATGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA CGAGGCCGAA AGCTGGATCA AGGAAAAGGA ACAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACG AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTCGAGG ACGAGCTCCG TGGGCTCGAT GCCCATCTGA AGCAGATCTT CCAGGAGGCT 
GAGGACATGG TTGCACAAAA GCAGTTTGGA CATCCACAGA TCGAGACCCG TGTCAAAGAA 
GTATCGGCTC AGTGGGACCA CTTGAAAGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTTTC CGGTGAAGAT GTGGGGCAAG ACGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTTCTAGAG GAGCTGGAGG AGAGCCGCGG GGTGATGGAG 
CACTTAGAGA ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTGACA 
AACCGTCTAC AGGCACTCAG GAAGCTCTAC CAGCAGGTGA TGGCCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACTGTGT TTGGAGAGTC AGACGCCTGT 
GAGCTGTGGA TGACTGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTTGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGCGT GAACCTTGCG GCCAACAACC TGGTAGAGAG CGGCCACCCA 
CGCAGTGGGG AGGTGAAACA ATATCAGGAC CGCCTAAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCGGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAAACCAG CAAGTGGATC GTGGACAAGA CGAAGGTTGT GGAGTCTACC 
AAGGACTTGG GGCAGGACCT AGCAGGAGTC ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGACG TATTGGCCAT CCGGGATCGT GTGAGCGCCT TGGAACGGGA GTCGCAGTAC 
TTGATGGAGT CACACCCTGA CCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTCGAG 
AAGATGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGTGAGGCC 
AGCAAGCTGC AGGCTTTCCT ACAAGACCTG GATGACTTCA AGGCCTGGCT GTCGATGGCC 
CAAAAGGCAG TGGCCTCTGA GGACATGCCT GAGTCACTCC CAGAGGCTGA GCAACTCCTA 
CAGCAGCATG CAGCCATTAA AGAGGAGATT GATGCTCACA AAGACGATTA CCACCGGGTC 
AAGGCCTCTG GGGAAAAAGT GATCGAAGGT CAAACTGACC CAGATTACCA GCTTCTGGGC 
CAGCGACTGG AGGGTCTAGA TACTGACTGG GATGCTCTGT GGCGGATGTG GGAGAGCCGA 
GGGAACACCC TCACCCAGTG CCTGGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACC TGGAGCCCCC AGACTCCCTA 
GCAGCTGCAG AGGCCGGCAT CCGAAAGTTT GAGGATTTCT TAGTGTCTAT GGAAAACAAC 
CGAGATAAAG TCCTGAGTCC TGTGGACTCC GGAAACATGC TGGTGTCTGA GGGCAACCTG 
TACTCGAACA AGATCAAAGA GAAGGTGCAG CTGATTGAAG ACAGGCACAT GAAGAACAAT 
GAGAAAGCCC AGGAGGTTAC AGCTCTACTG AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACATC 
TCCTATGACG AAGCCCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC ATTTATGGCA 
GAGCTGGCTT CACACCAAGG GTGGCTTGAG AACATCGTCG CGGAGGGAAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAC GGATGTGGTG TCAGAAAGGC TGGAAGCCCT GCACAAGCTC 
TGGGATGAGC TGCAGACCAC CACAAAGGCG AAGACGGAGC AACTCTCTGC TGCCCGGAGC 
TCTGACCTGC GCTTGCAAAC CCACGCCGAC CTTAGCAAAT GGATCAGCGC CATGGAGGAC 
CAGCTGCGAT CCGACGACCT GGGCAAGGAT CTGACCACTG TCAACCGGAT GTTGGTTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCA 
GAGGCACCCC CACTGGGAGC AGAGGCAGGA GACACGGACA TGAGCATTGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTATC CAAAGCCAAG 
CTACAGATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCGTTG 
GCGCAGTCAG CTGACTATGG CACTAATCTG CAAACAGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT CCTAGGCCAC GCACCACGGG TGGAGGATGT GCTACATCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCTTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCGGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGAAGCTC ACGAGGCACA GCAGTACTAC CTGGATGCGG GCGAGGCTGA AGCGTGGATC 
AGCGAGCAGG AGCTCTACGT GTTCTCTGAC GAACCCCCTA AGGACGAAGA GGGCGCCATC 
GTGATGCTCA AGCGGCACCT TCGGCAGCAG CGCACGGTGG AGGAATATGG AAGGAATATC 
AAACAGCTGG CTGGCCGCGC CCAGAGCCTG CTGTCTGCAG GCCACCCGGA GGGGGAGCAG 
ATCATCAGAC TTCAGGGGCA AGTGGATAAG CAGTATGCCG GGCTGAAGGA CATGGCTGAG 
GAACGGAGGC GGAAGCTAGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTGGAGC AGTGGATTAC GGAGAAAGAG ATGGTGGCCT CATCCCAAGA AATGGGGCAG 
GACTTTGACC ACGTGACTAT GCTCCGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCTATCGGGC AGGAGCGAGT AGACAATGTG AATTCTATCA TTGAGCGTCT CATCGATGCG 
GGCCACAGCG AGGCAGCCAC CATTGCAGAG TGGAAGGATG GGCTGAATGA CATGTGGGCA 
GATCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACGGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGTTG 
CCAGAGGACG TGGGGCTGGA CGCCAGCACA GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AGCTCCACCT CCTGGGTGTG CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC TGGGGAGAAG GCAGATGCTA TCCAGAGCAA GGAGCAGGAG 
GTGTCCGCGG CCTGGCAGGC CCTGCTGGAC GCGTGTGCTG GGCGCCGAGC TCAGCTGGTA 
GATACGGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTCTCCTC GGTGGAACTG 
CTCTTGAAGT ATCACCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AACTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCTTC AGACGAGATC 
CGGGAGAAAT TACAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAAGCC 
CGCTCTGACA GGCTCCACAT GCTGCTGGAG GTGTGCCAGT TCTCGAGGGA CGCTTCTGTG 
GCCGAGGCAT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACG 
GTGGACAGCG TGGAAAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGACA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCTCCCC AGGTCCACCA TGCGGCCACG GAGAGAACAT CCCCGGGGGA AGAAAGAGGG 
CCATGGCCTC AGGACCTGCA ACCACCACCC CTGCCAGGAC ACCACAAGGA TGAGCAGGAG 
AAATCCGTTG GGGATGAGAG GCCTGCCACA GAGCCCCTCT TTAAGGTCCT GGACACACCC 
CTGAGTGAGG GTGACGAACC CACAACGCTG CCAGCCCAGC GAGACCTCGG GCATACTGTG 
CAGATGGAGG GCTACTTGGG CAGAAAGCAC GACCTAGAGG GGCCCAACAA GAAGGCCTCT 
AACAGGTCCT GGAACAACCT GTACTGTGTG CTCAGGAACA GCCAGCTGAC CTTCTACAAG 
GACGCTAAGA ACCTGGCCCT CGGGGTGCCC TACCATGGAG AGGAGCCCCT GGCCCTGAGG 
CACGCCATCT GTGAGATTGC TGTCAACTAT AAGAAGAAGA AGCATGTGTT TAAGCTGAGG 
TTAAGCAACG GCAGTGAATG GCTTTTCCAT GGCAAGGATG AGGAGGAGAT GCTGTTGTGG 
CTACAGGGCA TGAGCACGGC CATCAACGAG TCCCAGAGCA TCCGTGTCAA GGCACAGAGT 
CTGCCCCTGC CTTCCCTGGC TGGTCCTGAT GCCAGCGTAG GCAAGAAGGA CAAGGAGAAG 
AGGTTTAGCT TCTTCCCCAA AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449631.1
CDS	379..7368
Translation	MTSATEFENVGNQPPYSRINARWDAPDDELDNDSSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRISDLYKDLRDGRMLIKLLEVLSGEMLPRPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPQVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFDVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTISVLNSRKFANSLSGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYQRELALRSELIRQEKLEQLARRFDRKAAMRETWLNENQRLVTQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVKALEDLAQELEKENYHDQKRISARKNNILRLWSYLQELLRSRRQRLEATLALQKLFQDMLHSIDWMDEIKAHILSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKTITAATLQFTEGKGYQPCDPQVIQDRVSHLEQCFSELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLKQIFQEAEDMVAQKQFGHPQIETRVKEVSAQWDHLKELAAFRKKDLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKEFLEELEESRGVMEHLENQAQGFPEEFRDSPDVTNRLQALRKLYQQVMAQAELRGHKLQEALDLYTVFGESDACELWMTEKGKWLDQMDIPNTLEDLEVVQHRFDILDQEMKTLMAQIDGVNLAANNLVESGHPRSGEVKQYQDRLNKRWQAFQAVVSEQREAVDSALRVNNYCVDCEETSKWIVDKTKVVESTKDLGQDLAGVIAIQRKLSGLERDVLAIRDRVSALERESQYLMESHPDQKEDIGQRQADVEKMWKGLQDALQGQELSLGEASKLQAFLQDLDDFKAWLSMAQKAVASEDMPESLPEAEQLLQQHAAIKEEIDAHKDDYHRVKASGEKVIEGQTDPDYQLLGQRLEGLDTDWDALWRMWESRGNTLTQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLAAAEAGIRKFEDFLVSMENNRDKVLSPVDSGNMLVSEGNLYSNKIKEKVQLIEDRHMKNNEKAQEVTALLKDNLELQNFLQNCKELTLWINDKLLTSPDISYDEARNLHNKWMKHQAFMAELASHQGWLENIVAEGRQLMAEKPQFTDVVSERLEALHKLWDELQTTTKAKTEQLSAARSSDLRLQTHADLSKWISAMEDQLRSDDLGKDLTTVNRMLVKLKRVEEQVNLRKEELEELFAEAPPLGAEAGDTDMSIEKRFLDLLEPLGRRKKQLELSKAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHAPRVEDVLHRGQELVKAAEIDCQDIEERLGHLQSSWDTLREAAAGRLQRLREAHEAQQYYLDAGEAEAWISEQELYVFSDEPPKDEEGAIVMLKRHLRQQRTVEEYGRNIKQLAGRAQSLLSAGHPEGEQIIRLQGQVDKQYAGLKDMAEERRRKLENMYHLFQLKREADDLEQWITEKEMVASSQEMGQDFDHVTMLRDKFRDFARETGAIGQERVDNVNSIIERLIDAGHSEAATIAEWKDGLNDMWADLLELIDTRMQLLAASYDLHRYFYTGTEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKADAIQSKEQEVSAAWQALLDACAGRRAQLVDTADKFRFFSMVRDLLSWMESIIRQIETQERPRDVSSVELLLKYHQGIKAEINTRAKNFSTCLELGESLLQRQHQASDEIREKLQQVISRRQEMNDKWEARSDRLHMLLEVCQFSRDASVAEAWLIAQEPYLASRDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQTPDRPTEEPGPQEEEGETAGEAPQVHHAATERTSPGEERGPWPQDLQPPPLPGHHKDEQEKSVGDERPATEPLFKVLDTPLSEGDEPTTLPAQRDLGHTVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSQLTFYKDAKNLALGVPYHGEEPLALRHAICEIAVNYKKKKHVFKLRLSNGSEWLFHGKDEEEMLLWLQGMSTAINESQSIRVKAQSLPLPSLAGPDASVGKKDKEKRFSFFPKKK

Target ORF information:

RefSeq Version	XM_017594142.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, erythrocytic (Sptb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594142.1

ORF Insert Sequence:

ATGACATCAG CCACGGAGTT TGAAAACGTG GGCAACCAGC CACCATACAG CCGGATTAAT 
GCCCGCTGGG ACGCCCCAGA TGATGAACTG GACAACGATA GCAGCTCAGC CAGGCTCTTT 
GAGAGGTCCC GGATCAAGGC CTTGGCAGAT GAGCGGGAAG TGGTTCAGAA GAAAACCTTT 
ACGAAATGGG TGAACTCCCA CCTGGCTCGA GTGTCCTGCC GCATCAGCGA TCTCTACAAG 
GACCTGCGGG ATGGACGCAT GCTCATCAAG CTGCTGGAGG TGCTCTCCGG AGAGATGCTG 
CCAAGGCCGA CCAAGGGCAA GATGCGCATC CACTGCCTGG AGAACGTGGA CAAAGCCCTG 
CAGTTCCTCA AGGAGCAGCG CGTGCACCTG GAAAACATGG GCTCCCACGA CATCGTGGAT 
GGCAACCACC GCCTGGTCCT GGGCCTCATC TGGACGATTA TCCTCCGATT CCAGATTCAA 
GACATTGTTG TCCAAACTCA AGAAGGTCGG GAGACACGCT CAGCCAAGGA TGCTCTGCTT 
CTGTGGTGCC AGATGAAGAC AGCCGGCTAC CCCCAAGTCA ACGTAACCAA CTTCACCTCC 
AGCTGGAAGG ACGGCCTGGC CTTCAATGCC CTAATTCATA AACACCGGCC TGACCTCATT 
GACTTTGACA AGCTAAAGGA CTCCAATGCC CGGCATAACT TGGAGCACGC ATTTGATGTG 
GCCGAGCGTC AGCTGGGCAT CATCCCACTC CTTGACCCCG AAGATGTCTT CACAGAAAAC 
CCAGACGAGA AGTCCATCAT CACCTACGTG GTAGCATTTT ACCACTACTT CTCCAAGATG 
AAGGTGCTAG CGGTGGAGGG CAAGAGAGTC GGCAAGGTCA TTGACCATGC CATTGAGACT 
GAGAAGATGA TTGAAAAGTA CAGTGGGCTA GCCTCAGACC TGCTCACCTG GATTGAGCAA 
ACCATTAGCG TCCTGAACAG CCGCAAGTTT GCCAACTCAT TGAGTGGCGT CCAGCAGCAG 
CTACAGGCCT TCAGCACCTA CCGCACTGTG GAGAAGCCAC CCAAGTTTCA AGAGAAGGGG 
AATCTGGAAG TCCTCCTGTT CACTATCCAA TCCCGAATGA GAGCCAACAA TCAGAAAGTG 
TACACGCCCC ACGATGGGAA GCTAGTGTCT GACATCAACA GGGCCTGGGA GAGCCTAGAG 
GAAGCAGAAT ACCAAAGGGA GCTGGCCCTG AGGAGCGAGC TCATTCGGCA GGAGAAACTG 
GAGCAGCTGG CGCGGCGCTT CGACCGTAAG GCAGCCATGA GAGAGACGTG GCTCAACGAA 
AACCAGCGCC TGGTGACCCA GGATAACTTC GGGTATGACC TGGCAGCTGT GGAGGCCGCC 
AAGAAGAAGC ACGAAGCCAT TGAGACCGAC ACGGCTGCCT ATGAGGAGCG GGTGAAGGCA 
CTGGAGGACC TGGCCCAGGA GCTGGAGAAG GAGAATTACC ATGACCAGAA GCGCATCAGT 
GCCAGGAAGA ACAATATCCT CCGTCTGTGG AGCTACCTGC AGGAGCTGCT GCGATCCCGG 
CGACAGCGGC TCGAGGCCAC CCTGGCACTG CAGAAGCTCT TCCAGGACAT GCTGCACAGT 
ATCGACTGGA TGGATGAGAT CAAGGCGCAC ATCTTGTCTG CTGAATTTGG GAAGCACTTG 
TTAGAGGTTG AAGACCTGCT ACAGAAACAC AAGCTAATGG AAGCCGACAT TGCTATCCAA 
GGGGACAAAG TGAAGACCAT CACCGCGGCC ACCCTGCAGT TCACTGAGGG GAAAGGGTAT 
CAGCCTTGTG ACCCCCAGGT CATCCAGGAC CGTGTCAGCC ACCTAGAGCA ATGCTTCAGT 
GAACTGAGCA ACATGGCAGC TGGACGGAAG GCTCAGCTGG AGCAGTCTAA GCGACTCTGG 
AAGTTCTTCT GGGAGATGGA CGAGGCCGAA AGCTGGATCA AGGAAAAGGA ACAGATCTAC 
TCCTCCCTGG ACTACGGCAA GGACCTGACG AGTGTGCTCA TCTTACAGCG CAAGCACAAG 
GCCTTCGAGG ACGAGCTCCG TGGGCTCGAT GCCCATCTGA AGCAGATCTT CCAGGAGGCT 
GAGGACATGG TTGCACAAAA GCAGTTTGGA CATCCACAGA TCGAGACCCG TGTCAAAGAA 
GTATCGGCTC AGTGGGACCA CTTGAAAGAG CTGGCTGCCT TTCGTAAGAA AGACCTGCAG 
GATGCGGAAA ACTTCTTCCA GTTCCAGGGT GATGCAGACG ACCTGAAGGC TTGGCTGCAA 
GACGCTCACC GGCTGCTTTC CGGTGAAGAT GTGGGGCAAG ACGAAGGAGC CACGAGGGCC 
CTGGGAAAGA AGCACAAGGA GTTTCTAGAG GAGCTGGAGG AGAGCCGCGG GGTGATGGAG 
CACTTAGAGA ACCAAGCCCA GGGCTTCCCC GAGGAGTTTC GAGATTCCCC AGATGTGACA 
AACCGTCTAC AGGCACTCAG GAAGCTCTAC CAGCAGGTGA TGGCCCAGGC AGAATTGCGT 
GGACACAAGC TGCAGGAAGC CCTGGACTTG TACACTGTGT TTGGAGAGTC AGACGCCTGT 
GAGCTGTGGA TGACTGAGAA GGGGAAGTGG CTGGACCAGA TGGATATCCC AAACACGCTG 
GAGGATCTGG AGGTTGTGCA GCACAGGTTT GACATCCTGG ACCAGGAGAT GAAGACCTTG 
ATGGCGCAGA TTGATGGCGT GAACCTTGCG GCCAACAACC TGGTAGAGAG CGGCCACCCA 
CGCAGTGGGG AGGTGAAACA ATATCAGGAC CGCCTAAACA AGAGGTGGCA GGCCTTCCAG 
GCAGTGGTGT CAGAGCAGAG AGAGGCTGTG GACTCGGCTC TCCGAGTAAA CAATTACTGT 
GTAGACTGTG AGGAAACCAG CAAGTGGATC GTGGACAAGA CGAAGGTTGT GGAGTCTACC 
AAGGACTTGG GGCAGGACCT AGCAGGAGTC ATTGCCATCC AGCGGAAGTT GTCAGGGTTG 
GAGCGGGACG TATTGGCCAT CCGGGATCGT GTGAGCGCCT TGGAACGGGA GTCGCAGTAC 
TTGATGGAGT CACACCCTGA CCAGAAAGAG GACATTGGCC AGCGGCAAGC AGATGTCGAG 
AAGATGTGGA AGGGCCTGCA AGATGCCTTG CAAGGTCAGG AGCTCTCCTT AGGTGAGGCC 
AGCAAGCTGC AGGCTTTCCT ACAAGACCTG GATGACTTCA AGGCCTGGCT GTCGATGGCC 
CAAAAGGCAG TGGCCTCTGA GGACATGCCT GAGTCACTCC CAGAGGCTGA GCAACTCCTA 
CAGCAGCATG CAGCCATTAA AGAGGAGATT GATGCTCACA AAGACGATTA CCACCGGGTC 
AAGGCCTCTG GGGAAAAAGT GATCGAAGGT CAAACTGACC CAGATTACCA GCTTCTGGGC 
CAGCGACTGG AGGGTCTAGA TACTGACTGG GATGCTCTGT GGCGGATGTG GGAGAGCCGA 
GGGAACACCC TCACCCAGTG CCTGGGCTTC CAGGAGTTCC AGAAAGATGC CAAGCAGGCT 
GAAGCCATCC TCAGCAACCA GGAATACACT CTGGCTCACC TGGAGCCCCC AGACTCCCTA 
GCAGCTGCAG AGGCCGGCAT CCGAAAGTTT GAGGATTTCT TAGTGTCTAT GGAAAACAAC 
CGAGATAAAG TCCTGAGTCC TGTGGACTCC GGAAACATGC TGGTGTCTGA GGGCAACCTG 
TACTCGAACA AGATCAAAGA GAAGGTGCAG CTGATTGAAG ACAGGCACAT GAAGAACAAT 
GAGAAAGCCC AGGAGGTTAC AGCTCTACTG AAAGACAACC TGGAGTTGCA GAACTTCCTC 
CAGAACTGCA AGGAGCTCAC TCTCTGGATC AATGACAAGC TGCTGACGTC TCCAGACATC 
TCCTATGACG AAGCCCGCAA CCTTCACAAC AAATGGATGA AACACCAGGC ATTTATGGCA 
GAGCTGGCTT CACACCAAGG GTGGCTTGAG AACATCGTCG CGGAGGGAAG GCAGCTGATG 
GCAGAGAAGC CCCAGTTCAC GGATGTGGTG TCAGAAAGGC TGGAAGCCCT GCACAAGCTC 
TGGGATGAGC TGCAGACCAC CACAAAGGCG AAGACGGAGC AACTCTCTGC TGCCCGGAGC 
TCTGACCTGC GCTTGCAAAC CCACGCCGAC CTTAGCAAAT GGATCAGCGC CATGGAGGAC 
CAGCTGCGAT CCGACGACCT GGGCAAGGAT CTGACCACTG TCAACCGGAT GTTGGTTAAG 
TTGAAGCGAG TGGAGGAACA AGTGAATTTG CGGAAGGAGG AGCTGGAGGA ACTCTTTGCA 
GAGGCACCCC CACTGGGAGC AGAGGCAGGA GACACGGACA TGAGCATTGA AAAGCGGTTC 
CTAGACCTTC TAGAACCTCT GGGGAGGAGG AAGAAGCAGC TGGAATTATC CAAAGCCAAG 
CTACAGATCA GCCGGGACTT AGAGGATGAG ACGCTCTGGG TGGAAGAGAG GCTGCCGTTG 
GCGCAGTCAG CTGACTATGG CACTAATCTG CAAACAGTGC AGCTGTTCAT GAAGAAGAAC 
CAGACCCTGC AGAATGAGAT CCTAGGCCAC GCACCACGGG TGGAGGATGT GCTACATCGA 
GGGCAGGAGC TGGTGAAGGC AGCCGAGATC GACTGCCAAG ACATCGAGGA GCGCTTGGGG 
CATCTGCAGA GCTCGTGGGA CACGCTGCGG GAGGCGGCAG CTGGCCGGCT GCAGCGCCTG 
CGGGAAGCTC ACGAGGCACA GCAGTACTAC CTGGATGCGG GCGAGGCTGA AGCGTGGATC 
AGCGAGCAGG AGCTCTACGT GTTCTCTGAC GAACCCCCTA AGGACGAAGA GGGCGCCATC 
GTGATGCTCA AGCGGCACCT TCGGCAGCAG CGCACGGTGG AGGAATATGG AAGGAATATC 
AAACAGCTGG CTGGCCGCGC CCAGAGCCTG CTGTCTGCAG GCCACCCGGA GGGGGAGCAG 
ATCATCAGAC TTCAGGGGCA AGTGGATAAG CAGTATGCCG GGCTGAAGGA CATGGCTGAG 
GAACGGAGGC GGAAGCTAGA GAACATGTAC CACCTGTTCC AGCTGAAGAG AGAGGCTGAC 
GACTTGGAGC AGTGGATTAC GGAGAAAGAG ATGGTGGCCT CATCCCAAGA AATGGGGCAG 
GACTTTGACC ACGTGACTAT GCTCCGTGAC AAGTTCCGAG ACTTTGCCCG GGAGACCGGG 
GCTATCGGGC AGGAGCGAGT AGACAATGTG AATTCTATCA TTGAGCGTCT CATCGATGCG 
GGCCACAGCG AGGCAGCCAC CATTGCAGAG TGGAAGGATG GGCTGAATGA CATGTGGGCA 
GATCTGCTGG AGCTCATTGA CACCCGCATG CAGCTGCTGG CTGCCTCCTA CGACCTGCAC 
CGCTATTTCT ACACGGGCAC CGAGATCCTG GGCCTCATAG ATGAGAAGCA CAGAGAGTTG 
CCAGAGGACG TGGGGCTGGA CGCCAGCACA GCTGAGTCCT TCCACCGGGT CCACACTGCC 
TTCGAGCGGG AGCTCCACCT CCTGGGTGTG CAGGTGCAGC AGTTCCAGGA CGTGGCCACC 
CGACTGCAGA CAGCGTATGC TGGGGAGAAG GCAGATGCTA TCCAGAGCAA GGAGCAGGAG 
GTGTCCGCGG CCTGGCAGGC CCTGCTGGAC GCGTGTGCTG GGCGCCGAGC TCAGCTGGTA 
GATACGGCAG ACAAGTTCCG CTTCTTCAGC ATGGTGCGTG ACCTCCTCTC CTGGATGGAG 
AGCATCATCC GGCAGATCGA GACCCAGGAG AGACCCAGGG ATGTCTCCTC GGTGGAACTG 
CTCTTGAAGT ATCACCAGGG CATCAAGGCA GAGATCAATA CCAGGGCCAA GAACTTCAGC 
ACCTGCCTAG AACTTGGGGA GTCCCTGCTA CAGAGGCAAC ACCAGGCTTC AGACGAGATC 
CGGGAGAAAT TACAGCAAGT GATATCCAGG AGGCAGGAGA TGAATGACAA GTGGGAAGCC 
CGCTCTGACA GGCTCCACAT GCTGCTGGAG GTGTGCCAGT TCTCGAGGGA CGCTTCTGTG 
GCCGAGGCAT GGCTGATTGC CCAGGAGCCC TACCTGGCCA GCCGGGACTT TGGACATACG 
GTGGACAGCG TGGAAAAGCT CATCAAGAGG CATGAGGCTT TTGAGAAGTC CACAGCCAGC 
TGGGCAGAGC GCTTTGCTGC CCTGGAGAAG CCCACCACGC TTGAGCTTAA GGAACGCCAG 
ACTCCAGACA GACCCACAGA GGAACCTGGG CCTCAAGAGG AGGAAGGCGA GACAGCTGGC 
GAGGCTCCCC AGGTCCACCA TGCGGCCACG GAGAGAACAT CCCCGGGGGA AGAAAGAGGG 
CCATGGCCTC AGGACCTGCA ACCACCACCC CTGCCAGGAC ACCACAAGGA TGAGCAGGAG 
AAATCCGTTG GGGATGAGAG GCCTGCCACA GAGCCCCTCT TTAAGGTCCT GGACACACCC 
CTGAGTGAGG GTGACGAACC CACAACGCTG CCAGCCCAGC GAGACCTCGG GCATACTGTG 
CAGATGGAGG GCTACTTGGG CAGAAAGCAC GACCTAGAGG GGCCCAACAA GAAGGCCTCT 
AACAGGTCCT GGAACAACCT GTACTGTGTG CTCAGGAACA GCCAGCTGAC CTTCTACAAG 
GACGCTAAGA ACCTGGCCCT CGGGGTGCCC TACCATGGAG AGGAGCCCCT GGCCCTGAGG 
CACGCCATCT GTGAGATTGC TGTCAACTAT AAGAAGAAGA AGCATGTGTT TAAGCTGAGG 
TTAAGCAACG GCAGTGAATG GCTTTTCCAT GGCAAGGATG AGGAGGAGAT GCTGTTGTGG 
CTACAGGGCA TGAGCACGGC CATCAACGAG TCCCAGAGCA TCCGTGTCAA GGCACAGAGT 
CTGCCCCTGC CTTCCCTGGC TGGTCCTGAT GCCAGCGTAG GCAAGAAGGA CAAGGAGAAG 
AGGTTTAGCT TCTTCCCCAA AAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The actin binding domain of βI-spectrin regulates the morphological and functional dynamics of dendritic spines.
PloS one6(1)e16197(2011 Jan)
Nestor MW,Cai X,Stone MR,Bloch RJ,Thompson SM

The carboxyterminal EF domain of erythroid alpha-spectrin is necessary for optimal spectrin-actin binding.
Blood116(14)2600-7(2010 Oct)
Korsgren C,Lux SE

Novel beta-spectrin mutations in hereditary spherocytosis associated with decreased levels of mRNA.
British journal of haematology146(3)326-32(2009 Aug)
Maciag M,Płochocka D,Adamowicz-Salach A,Burzyńska B

Targeted deletion of alpha-adducin results in absent beta- and gamma-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice.
Blood112(10)4298-307(2008 Nov)
Robledo RF,Ciciotte SL,Gwynn B,Sahr KE,Gilligan DM,Mohandas N,Peters LL

Identification and functional characterization of protein 4.1R and actin-binding sites in erythrocyte beta spectrin: regulation of the interactions by phosphatidylinositol-4,5-bisphosphate.
Biochemistry44(31)10681-8(2005 Aug)
An X,Debnath G,Guo X,Liu S,Lux SE,Baines A,Gratzer W,Mohandas N

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Sptb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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