Polr1e cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr1e
Entrez Gene ID	313245
Full Name	RNA polymerase I subunit E
Synonyms	Praf1,RGD1565773
General protein information	Preferred Names RNA polymerase I subunit E Names DNA-directed RNA polymerase I subunit RPA49 polymerase (RNA) I associated factor 1 polymerase (RNA) I polypeptide E polymerase (RNA) I subunit E
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107938.1	NP_001101408.1	DNA-directed RNA polymerase I subunit RPA49

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Polr1e	NP_001101408.1
Xenopus tropicalis (tropical clawed frog)	polr1e	NP_001120306.1
Gallus gallus (chicken)	POLR1E	XP_424416.3
Danio rerio (zebrafish)	polr1e	NP_001018540.1
Mus musculus (house mouse)	Polr1e	NP_073722.1
Homo sapiens (human)	POLR1E	NP_071935.1
Pan troglodytes (chimpanzee)	POLR1E	XP_001169824.1
Canis lupus familiaris (dog)	POLR1E	XP_531998.2
Bos taurus (cattle)	POLR1E	NP_001029580.1

Gene Ontology
Bibliography

Related articles in PubMed

Conservation between the RNA polymerase I, II, and III transcription initiation machineries.
Vannini A, Cramer P
Molecular cell45(4)439-46(2012 Feb)

RNA polymerase I activity is regulated at multiple steps in the transcription cycle: recent insights into factors that influence transcription elongation.
Schneider DA
Gene493(2)176-84(2012 Feb)

PAF67, a novel protein that is associated with the initiation-competent form of RNA polymerase I.
Seither P, Iben S, Thiry M, Grummt I
Biological chemistry382(8)1163-70(2001 Aug)

RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription.
Hanada K, Song CZ, Yamamoto K, Yano K, Maeda Y, Yamaguchi K, Muramatsu M
The EMBO journal15(9)2217-26(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06329	NM_001107938.1 Latest version!	Rattus norvegicus RNA polymerase I subunit E (Polr1e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr1e ( NM_001107938.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101408.1 Rattus norvegicus RNA polymerase I subunit E (Polr1e), mRNA.

Price & Availability↑

CloneID	ORa06329
Clone ID Related Accession (Same CDS sequence)	NM_001107938.1
Accession Version	NM_001107938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	DNA-directed RNA polymerase I subunit RPA49
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473962.1. On or before Oct 4, 2007 this sequence version replaced XM_233004.4, XM_001065234.1. ##Evidence-Data-START## Transcript exon combination :: BC158699.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACGC CGGATTCTCC CGGGATGGAT GGCCAGACTG GAGACACGGA GACAGAGGTG 
CTACAGAGTG CAAGATGGCA GTACTGTGGA GAGCCCGACG ACAGGCAGAA AGCTGTGCTG 
GTCCAATTCT CCAATGGGAA GCTGCAGAAT CCAGGCAACA TGCGCTTCAC CTTGTACAAC 
AGCACCGACT TGGTAAACCC CAGGCAGAAG TGCCATCGGA TCCTGGCCGC TGAGACAGAC 
AGGCTCTCCT ATGTTGGAAA CAATTTTGGG ACTGGCGCCC TCAAATGCAA CACTCTTTGC 
AGACACTTTG TGGGCATTCT GAATAAGACC TCTGGCCAAA TGGAAGTCTA TGATGCGGAA 
GTATTCAACA TGCAGCCCCT GTTTGCTGAA GACTCTATTG AGCATGGGCC TCCTCTGGAG 
AATCAGAACA AGACCTTCAG GGACAAGTTG GATTCTTGCA TTGAAGCCTT TGGAAGCACC 
AAGCAGAAGC GGTCCCTGAA CTCCCGGAGA ATGAACAAAG TTGGCAGCGA GTCTTTGAAT 
TTCACAGTAG CTAAAGCCGC AGAGAGTATC ATTGACACGA AGGGCGTGAG TGCTTTGGTC 
AGCGATGCCA TGCAAGATGA CTTGCAAAAT GACTCCCTCT ACCTTCCTCC CTGCCATGCT 
GATGCCACCA AGCCCGAAGA CGTGTATAGA TTTGAAGACA TCCTGTCCCC GGCCGAGTAT 
GATGCTCTTG AAAGCCCATC CGAAGCCTTC AGAAAGGTCA TGTCTGAAGA CATACTGAAG 
ATGGTGGAAG AGAACAGCCA CTGCTCCTTC ATCATAGAAA TGCTGAAGTC TTTGCCAGCA 
GACGAGGTGC AGCGAAACCG GCAAGCCCGG AGCATTTGGT TCCTAGATGC CCTCCTCAGA 
TTCCGAGCTC AGAAAGTGAT AAAAGGGAAA AGTGCCTTAG GACCTGGGAT CCCGCACATC 
ATCAACACCA AGCTGCTGAA GCAGTTCACC TGCTTGACCT ACAACAACGG CAGTTTACGG 
AACTTAATCT CGAGTTCTAT GAAGGCCAAG ATCACTGCAT ATGCAATCAT CCTTGCTCTG 
CACATAAATA ACTTCCAGAT TGATCTGACG GTGTTACAGA GGGACTTGAA GCTGAGTGAG 
AAAAGGATGA TTGAGATAGC GAGAGCCATG AGGCTGAAGA TCTCCAAAAG AAAGGTGTCC 
CTGGCAGATG GCAGGGAAGA GGATCATAGA CTGGGCACTC TGTCCGTCCC ACTGCCTCCG 
GCCCAGACCT CAGACCGACA GTCGAAGCGG AAAAAAATGA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101408.1
CDS	27..1331
Translation	MATPDSPGMDGQTGDTETEVLQSARWQYCGEPDDRQKAVLVQFSNGKLQNPGNMRFTLYNSTDLVNPRQKCHRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAEVFNMQPLFAEDSIEHGPPLENQNKTFRDKLDSCIEAFGSTKQKRSLNSRRMNKVGSESLNFTVAKAAESIIDTKGVSALVSDAMQDDLQNDSLYLPPCHADATKPEDVYRFEDILSPAEYDALESPSEAFRKVMSEDILKMVEENSHCSFIIEMLKSLPADEVQRNRQARSIWFLDALLRFRAQKVIKGKSALGPGIPHIINTKLLKQFTCLTYNNGSLRNLISSSMKAKITAYAIILALHINNFQIDLTVLQRDLKLSEKRMIEIARAMRLKISKRKVSLADGREEDHRLGTLSVPLPPAQTSDRQSKRKKMS

Target ORF information:

RefSeq Version	NM_001107938.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA polymerase I subunit E (Polr1e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107938.1

ORF Insert Sequence:

ATGGCTACGC CGGATTCTCC CGGGATGGAT GGCCAGACTG GAGACACGGA GACAGAGGTG 
CTACAGAGTG CAAGATGGCA GTACTGTGGA GAGCCCGACG ACAGGCAGAA AGCTGTGCTG 
GTCCAATTCT CCAATGGGAA GCTGCAGAAT CCAGGCAACA TGCGCTTCAC CTTGTACAAC 
AGCACCGACT TGGTAAACCC CAGGCAGAAG TGCCATCGGA TCCTGGCCGC TGAGACAGAC 
AGGCTCTCCT ATGTTGGAAA CAATTTTGGG ACTGGCGCCC TCAAATGCAA CACTCTTTGC 
AGACACTTTG TGGGCATTCT GAATAAGACC TCTGGCCAAA TGGAAGTCTA TGATGCGGAA 
GTATTCAACA TGCAGCCCCT GTTTGCTGAA GACTCTATTG AGCATGGGCC TCCTCTGGAG 
AATCAGAACA AGACCTTCAG GGACAAGTTG GATTCTTGCA TTGAAGCCTT TGGAAGCACC 
AAGCAGAAGC GGTCCCTGAA CTCCCGGAGA ATGAACAAAG TTGGCAGCGA GTCTTTGAAT 
TTCACAGTAG CTAAAGCCGC AGAGAGTATC ATTGACACGA AGGGCGTGAG TGCTTTGGTC 
AGCGATGCCA TGCAAGATGA CTTGCAAAAT GACTCCCTCT ACCTTCCTCC CTGCCATGCT 
GATGCCACCA AGCCCGAAGA CGTGTATAGA TTTGAAGACA TCCTGTCCCC GGCCGAGTAT 
GATGCTCTTG AAAGCCCATC CGAAGCCTTC AGAAAGGTCA TGTCTGAAGA CATACTGAAG 
ATGGTGGAAG AGAACAGCCA CTGCTCCTTC ATCATAGAAA TGCTGAAGTC TTTGCCAGCA 
GACGAGGTGC AGCGAAACCG GCAAGCCCGG AGCATTTGGT TCCTAGATGC CCTCCTCAGA 
TTCCGAGCTC AGAAAGTGAT AAAAGGGAAA AGTGCCTTAG GACCTGGGAT CCCGCACATC 
ATCAACACCA AGCTGCTGAA GCAGTTCACC TGCTTGACCT ACAACAACGG CAGTTTACGG 
AACTTAATCT CGAGTTCTAT GAAGGCCAAG ATCACTGCAT ATGCAATCAT CCTTGCTCTG 
CACATAAATA ACTTCCAGAT TGATCTGACG GTGTTACAGA GGGACTTGAA GCTGAGTGAG 
AAAAGGATGA TTGAGATAGC GAGAGCCATG AGGCTGAAGA TCTCCAAAAG AAAGGTGTCC 
CTGGCAGATG GCAGGGAAGA GGATCATAGA CTGGGCACTC TGTCCGTCCC ACTGCCTCCG 
GCCCAGACCT CAGACCGACA GTCGAAGCGG AAAAAAATGA GCTAG