Atg7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg7
Entrez Gene ID	312647
Full Name	autophagy related 7
Synonyms	Apg7l
General protein information	Preferred Names autophagy related 7 Names ubiquitin-like modifier-activating enzyme ATG7 ATG12-activating enzyme E1 ATG7 ATG7 autophagy related 7 homolog autophagy 7-like autophagy related 7 homolog autophagy-related protein 7 ubiquitin-activating enzyme E1-like protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q42

mRNA and Protein(s)

mRNA	Protein	Name
XM_006237034.3	XP_006237096.1	ubiquitin-like modifier-activating enzyme ATG7 isoform X3
XM_008763239.2	XP_008761461.1	ubiquitin-like modifier-activating enzyme ATG7 isoform X1
NM_001012097.1	NP_001012097.1	ubiquitin-like modifier-activating enzyme ATG7
XM_006237033.3	XP_006237095.1	ubiquitin-like modifier-activating enzyme ATG7 isoform X2

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_07297	XP_003715525.1
Macaca mulatta (Rhesus monkey)	ATG7	XP_001088170.1
Canis lupus familiaris (dog)	ATG7	XP_849849.2
Drosophila melanogaster (fruit fly)	Atg7	NP_611350.1
Caenorhabditis elegans (roundworm)	atg-7	NP_502064.1
Saccharomyces cerevisiae (baker's yeast)	ATG7	NP_012041.1
Xenopus tropicalis (tropical clawed frog)	atg7	NP_001123394.1
Kluyveromyces lactis	KLLA0A05137g	XP_451226.1
Mus musculus (house mouse)	Atg7	NP_001240646.1
Neurospora crassa	NCU06672	XP_960919.1
Oryza sativa (rice)	Os01g0614900	NP_001043569.1
Danio rerio (zebrafish)	atg7	XP_005172819.1
Schizosaccharomyces pombe (fission yeast)	atg7	NP_596084.1
Pan troglodytes (chimpanzee)	ATG7	XP_003309672.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010303	XP_318213.4
Eremothecium gossypii	AGOS_AER298C	NP_985155.1
Rattus norvegicus (Norway rat)	Atg7	NP_001012097.1
Arabidopsis thaliana (thale cress)	APG7	NP_568652.1
Homo sapiens (human)	ATG7	NP_006386.1
Bos taurus (cattle)	ATG7	NP_001136439.1
Gallus gallus (chicken)	ATG7	NP_001025763.1

Gene Ontology
Bibliography

Related articles in PubMed

Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY1.
Toledo M, Batista-Gonzalez A, Merheb E, Aoun ML, Tarabra E, Feng D, Sarparanta J, Merlo P, Botrè F, Schwartz GJ, Pessin JE, Singh R
Cell metabolism28(2)268-281.e4(2018 08)

Oxidation of Atg3 and Atg7 mediates inhibition of autophagy.
Frudd K, Burgoyne T, Burgoyne JR
Nature communications9(1)95(2018 01)

miR-96 attenuates status epilepticus-induced brain injury by directly targeting Atg7 and Atg16L1.
Gan J, Cai Q, Qu Y, Zhao F, Wan C, Luo R, Mu D
Scientific reports7(1)10270(2017 08)

Geldanamycin Reduces Aβ-Associated Anxiety and Depression, Concurrent with Autophagy Provocation.
Zare N, Khalifeh S, Khodagholi F, Shahamati SZ, Motamedi F, Maghsoudi N
Journal of molecular neuroscience : MN57(3)317-24(2015 Nov)

Emerging role for autophagy in the removal of aggresomes in Schwann cells.
Fortun J, Dunn WA Jr, Joy S, Li J, Notterpek L
The Journal of neuroscience : the official journal of the Society for Neuroscience23(33)10672-80(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12829	NM_001012097.1 Latest version!	Rattus norvegicus autophagy related 7 (Atg7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa12829	XM_006237033.3 Latest version!	Rattus norvegicus autophagy related 7 (Atg7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa27566	XM_006237034.3 Latest version!	Rattus norvegicus autophagy related 7 (Atg7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa47146	XM_008763239.2 Latest version!	Rattus norvegicus autophagy related 7 (Atg7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg7 ( NM_001012097.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012097.1 Rattus norvegicus autophagy related 7 (Atg7), mRNA.

Price & Availability↑

CloneID	ORa12829
Clone ID Related Accession (Same CDS sequence)	NM_001012097.1 , XM_006237033.3
Accession Version	NM_001012097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme ATG7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC082059.1. On Feb 11, 2005 this sequence version replaced XM_232276.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGACC CTGGACTGTC CAAGCTGCAG TTTGCCCCCT TTAATAGTGC CCTGGACGTT 
GGCTTCTGGC ACGAACTGAC CCAGAAGAAG TTGAACGAGT ATCGCCTGGA CGAGGCACCC 
AAAGACATCA AGGGCTATTA CTACAATGGT GACTCTGCCG GCCTCCCCAC CCGCTTGACA 
TTGGAGTTCA GTGCTTTTGA CATGAGCGCC CCCACGCCTG CCCGCTGCTG CCCGGCCATG 
GGAACCCTGC ACAACACCAA CACACTTGAG GCTTTTAAGA CTGCAGACAA GAAGCTCCTT 
CTGGAGCAGT CAGCCAATGA GATCTGGGAA GCCATAAAGT CAGGTGCTGC TCTCGAAAAC 
CCCATGCTCC TCAACAAGTT TCTGCTCTTG ACCTTCGCGG ACCTAAAGAA GTACCACTTC 
TACTACTGGT TTTGCTGCCC TGCCCTCTGT CTTCCTGAGA GCATCCCTCT AATCCGGGGA 
CCTGTGGGCT TGGATCAAAG GCTTTCACCA AAACAGATCC AGGCCCTGGA GCATGCCTAC 
GATGACCTGT GTCGAACTGA AGGCGTCACA GCCCTGCCAT ACTTCTTATT CAAGTACGAT 
GACGACACTG TGCTGGTCTC CTTGCTCAAA CACTACAGTG ATTTCTTCCA AGGTCAAAGG 
ACAAAGTTAA CAGTCGGTGT GTACGATCCC TGTAACCTAA CCCAGCACCC TGGATGGCCT 
TTGAGGAATT TTTTGGTCCT GGCAGCCCAC AGATGGAGCG GCAGTTTCCA GTCTGTTGAA 
GTTCTCTGCT TTCGGGACCG CACCATGCAG GGAGCAAGAG ATGTGACACA CAGCATCATC 
TTTGAAGTGA AACTTCCAGA AATGGCGTTT AGCCCAGATT GTCCTAAGGC AGTTGGCTGG 
GAGAAGAACC AGAAAGGAGG CATGGGTCCG AGGATGGTGA ACCTCAGCGG ATGTATGGAC 
CCCAAAAGGC TGGCTGAGTC ATCCGTGGAT CTGAATCTCA AGCTGATGTG CTGGCGGTTG 
GTCCCCACCT TGGACTTGGA CAAGGTCGTG TCTGTCAAGT GCCTGCTGCT GGGAGCTGGT 
ACCTTGGGGT GTAATGTGGC TAGAACACTG ATGGGCTGGG GCGTCAGACA CGTCACGTTT 
GTGGACAACG CCAAGATCTC CTACTCCAAT CCCGTGAGGC AGCCTCTGTA TGAATTTGAA 
GATTGTCTAG GGGGTGGCAA GCCCAAGGCC CTGGCTGCAG CAGAGCGGCT CCAGAAAATA 
TTTCCCGGAG TGAATGCCAG CGGGTTCAAC ATGAGCATCC CCATGCCCGG ACACCCTGTG 
AACTTCTCTG ACGTCACGAT GGAGCAGGCC CGCAGAGATG TGGAGCAGCT GGAGGAACTC 
ATCGATAGCC ACGATGTCAT CTTCCTGCTA ATGGACACCA GGGAGAGCCG ATGGCTTCCT 
ACTGTTATTG CAGCCAGCAA GCGAAAGCTG GTCATCAATG CTGCCTTGGG GTTTGACACC 
TTTGTTGTCA TGAGACATGG CCTGAAGAAA CCCAAGCAGC AGGGAGCCGG GGACCTGTGC 
CCAAGCCATC TTGTAGCACC TGCTGACCTG GGCTCCTCAC TTTTTGCCAA CATCCCTGGA 
TACAAGCTTG GCTGCTACTT CTGCAATGAT GTGGTGGCTC CAGGAGATTC CACCAGAGAC 
CGGACTCTGG ACCAGCAGTG CACAGTGAGC CGCCCAGGGC TGGCCGTGAT TGCAGGAGCC 
CTGGCTGTGG AGCTGATGGT TTCTGTCTTG CAGCATCCTG AGGGGGGCTA CGCCATCGCC 
AGCAGCAGTG ATGACCGCAT GAATGAGCCT CCCACCTCGC TGGGGCTTGT ACCTCACCAG 
ATCCGGGGCT TTCTGTCACG GTTCGATAAT GTCCTTCCTG TCAGCCTGGC ATTTGATAAA 
TGTACAGCCT GTTCATCCAA AGTTCTTGAT CAATATGAGC AAGAAGGATT CACCTTCCTG 
GCCAAGGTGT TTAACTCTTC ACATTCCTTC TTAGAAGATT TGACTGGTCT TACACTGCTC 
CACCAAGAGA CCCAAGCTGC TGAGATCTGG GACATGAGTG ACGAGGAGAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012097.1
CDS	16..2112
Translation	MGDPGLSKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAFDMSAPTPARCCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGAALENPMLLNKFLLLTFADLKKYHFYYWFCCPALCLPESIPLIRGPVGLDQRLSPKQIQALEHAYDDLCRTEGVTALPYFLFKYDDDTVLVSLLKHYSDFFQGQRTKLTVGVYDPCNLTQHPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAAERLQKIFPGVNASGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEELIDSHDVIFLLMDTRESRWLPTVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEQEGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDEETV

Target ORF information:

RefSeq Version	NM_001012097.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy related 7 (Atg7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012097.1

ORF Insert Sequence:

ATGGGGGACC CTGGACTGTC CAAGCTGCAG TTTGCCCCCT TTAATAGTGC CCTGGACGTT 
GGCTTCTGGC ACGAACTGAC CCAGAAGAAG TTGAACGAGT ATCGCCTGGA CGAGGCACCC 
AAAGACATCA AGGGCTATTA CTACAATGGT GACTCTGCCG GCCTCCCCAC CCGCTTGACA 
TTGGAGTTCA GTGCTTTTGA CATGAGCGCC CCCACGCCTG CCCGCTGCTG CCCGGCCATG 
GGAACCCTGC ACAACACCAA CACACTTGAG GCTTTTAAGA CTGCAGACAA GAAGCTCCTT 
CTGGAGCAGT CAGCCAATGA GATCTGGGAA GCCATAAAGT CAGGTGCTGC TCTCGAAAAC 
CCCATGCTCC TCAACAAGTT TCTGCTCTTG ACCTTCGCGG ACCTAAAGAA GTACCACTTC 
TACTACTGGT TTTGCTGCCC TGCCCTCTGT CTTCCTGAGA GCATCCCTCT AATCCGGGGA 
CCTGTGGGCT TGGATCAAAG GCTTTCACCA AAACAGATCC AGGCCCTGGA GCATGCCTAC 
GATGACCTGT GTCGAACTGA AGGCGTCACA GCCCTGCCAT ACTTCTTATT CAAGTACGAT 
GACGACACTG TGCTGGTCTC CTTGCTCAAA CACTACAGTG ATTTCTTCCA AGGTCAAAGG 
ACAAAGTTAA CAGTCGGTGT GTACGATCCC TGTAACCTAA CCCAGCACCC TGGATGGCCT 
TTGAGGAATT TTTTGGTCCT GGCAGCCCAC AGATGGAGCG GCAGTTTCCA GTCTGTTGAA 
GTTCTCTGCT TTCGGGACCG CACCATGCAG GGAGCAAGAG ATGTGACACA CAGCATCATC 
TTTGAAGTGA AACTTCCAGA AATGGCGTTT AGCCCAGATT GTCCTAAGGC AGTTGGCTGG 
GAGAAGAACC AGAAAGGAGG CATGGGTCCG AGGATGGTGA ACCTCAGCGG ATGTATGGAC 
CCCAAAAGGC TGGCTGAGTC ATCCGTGGAT CTGAATCTCA AGCTGATGTG CTGGCGGTTG 
GTCCCCACCT TGGACTTGGA CAAGGTCGTG TCTGTCAAGT GCCTGCTGCT GGGAGCTGGT 
ACCTTGGGGT GTAATGTGGC TAGAACACTG ATGGGCTGGG GCGTCAGACA CGTCACGTTT 
GTGGACAACG CCAAGATCTC CTACTCCAAT CCCGTGAGGC AGCCTCTGTA TGAATTTGAA 
GATTGTCTAG GGGGTGGCAA GCCCAAGGCC CTGGCTGCAG CAGAGCGGCT CCAGAAAATA 
TTTCCCGGAG TGAATGCCAG CGGGTTCAAC ATGAGCATCC CCATGCCCGG ACACCCTGTG 
AACTTCTCTG ACGTCACGAT GGAGCAGGCC CGCAGAGATG TGGAGCAGCT GGAGGAACTC 
ATCGATAGCC ACGATGTCAT CTTCCTGCTA ATGGACACCA GGGAGAGCCG ATGGCTTCCT 
ACTGTTATTG CAGCCAGCAA GCGAAAGCTG GTCATCAATG CTGCCTTGGG GTTTGACACC 
TTTGTTGTCA TGAGACATGG CCTGAAGAAA CCCAAGCAGC AGGGAGCCGG GGACCTGTGC 
CCAAGCCATC TTGTAGCACC TGCTGACCTG GGCTCCTCAC TTTTTGCCAA CATCCCTGGA 
TACAAGCTTG GCTGCTACTT CTGCAATGAT GTGGTGGCTC CAGGAGATTC CACCAGAGAC 
CGGACTCTGG ACCAGCAGTG CACAGTGAGC CGCCCAGGGC TGGCCGTGAT TGCAGGAGCC 
CTGGCTGTGG AGCTGATGGT TTCTGTCTTG CAGCATCCTG AGGGGGGCTA CGCCATCGCC 
AGCAGCAGTG ATGACCGCAT GAATGAGCCT CCCACCTCGC TGGGGCTTGT ACCTCACCAG 
ATCCGGGGCT TTCTGTCACG GTTCGATAAT GTCCTTCCTG TCAGCCTGGC ATTTGATAAA 
TGTACAGCCT GTTCATCCAA AGTTCTTGAT CAATATGAGC AAGAAGGATT CACCTTCCTG 
GCCAAGGTGT TTAACTCTTC ACATTCCTTC TTAGAAGATT TGACTGGTCT TACACTGCTC 
CACCAAGAGA CCCAAGCTGC TGAGATCTGG GACATGAGTG ACGAGGAGAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg7 ( XM_006237033.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237095.1 Rattus norvegicus autophagy related 7 (Atg7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12829
Clone ID Related Accession (Same CDS sequence)	NM_001012097.1 , XM_006237033.3
Accession Version	XM_006237033.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme ATG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACC CTGGACTGTC CAAGCTGCAG TTTGCCCCCT TTAATAGTGC CCTGGACGTT 
GGCTTCTGGC ACGAACTGAC CCAGAAGAAG TTGAACGAGT ATCGCCTGGA CGAGGCACCC 
AAAGACATCA AGGGCTATTA CTACAATGGT GACTCTGCCG GCCTCCCCAC CCGCTTGACA 
TTGGAGTTCA GTGCTTTTGA CATGAGCGCC CCCACGCCTG CCCGCTGCTG CCCGGCCATG 
GGAACCCTGC ACAACACCAA CACACTTGAG GCTTTTAAGA CTGCAGACAA GAAGCTCCTT 
CTGGAGCAGT CAGCCAATGA GATCTGGGAA GCCATAAAGT CAGGTGCTGC TCTCGAAAAC 
CCCATGCTCC TCAACAAGTT TCTGCTCTTG ACCTTCGCGG ACCTAAAGAA GTACCACTTC 
TACTACTGGT TTTGCTGCCC TGCCCTCTGT CTTCCTGAGA GCATCCCTCT AATCCGGGGA 
CCTGTGGGCT TGGATCAAAG GCTTTCACCA AAACAGATCC AGGCCCTGGA GCATGCCTAC 
GATGACCTGT GTCGAACTGA AGGCGTCACA GCCCTGCCAT ACTTCTTATT CAAGTACGAT 
GACGACACTG TGCTGGTCTC CTTGCTCAAA CACTACAGTG ATTTCTTCCA AGGTCAAAGG 
ACAAAGTTAA CAGTCGGTGT GTACGATCCC TGTAACCTAA CCCAGCACCC TGGATGGCCT 
TTGAGGAATT TTTTGGTCCT GGCAGCCCAC AGATGGAGCG GCAGTTTCCA GTCTGTTGAA 
GTTCTCTGCT TTCGGGACCG CACCATGCAG GGAGCAAGAG ATGTGACACA CAGCATCATC 
TTTGAAGTGA AACTTCCAGA AATGGCGTTT AGCCCAGATT GTCCTAAGGC AGTTGGCTGG 
GAGAAGAACC AGAAAGGAGG CATGGGTCCG AGGATGGTGA ACCTCAGCGG ATGTATGGAC 
CCCAAAAGGC TGGCTGAGTC ATCCGTGGAT CTGAATCTCA AGCTGATGTG CTGGCGGTTG 
GTCCCCACCT TGGACTTGGA CAAGGTCGTG TCTGTCAAGT GCCTGCTGCT GGGAGCTGGT 
ACCTTGGGGT GTAATGTGGC TAGAACACTG ATGGGCTGGG GCGTCAGACA CGTCACGTTT 
GTGGACAACG CCAAGATCTC CTACTCCAAT CCCGTGAGGC AGCCTCTGTA TGAATTTGAA 
GATTGTCTAG GGGGTGGCAA GCCCAAGGCC CTGGCTGCAG CAGAGCGGCT CCAGAAAATA 
TTTCCCGGAG TGAATGCCAG CGGGTTCAAC ATGAGCATCC CCATGCCCGG ACACCCTGTG 
AACTTCTCTG ACGTCACGAT GGAGCAGGCC CGCAGAGATG TGGAGCAGCT GGAGGAACTC 
ATCGATAGCC ACGATGTCAT CTTCCTGCTA ATGGACACCA GGGAGAGCCG ATGGCTTCCT 
ACTGTTATTG CAGCCAGCAA GCGAAAGCTG GTCATCAATG CTGCCTTGGG GTTTGACACC 
TTTGTTGTCA TGAGACATGG CCTGAAGAAA CCCAAGCAGC AGGGAGCCGG GGACCTGTGC 
CCAAGCCATC TTGTAGCACC TGCTGACCTG GGCTCCTCAC TTTTTGCCAA CATCCCTGGA 
TACAAGCTTG GCTGCTACTT CTGCAATGAT GTGGTGGCTC CAGGAGATTC CACCAGAGAC 
CGGACTCTGG ACCAGCAGTG CACAGTGAGC CGCCCAGGGC TGGCCGTGAT TGCAGGAGCC 
CTGGCTGTGG AGCTGATGGT TTCTGTCTTG CAGCATCCTG AGGGGGGCTA CGCCATCGCC 
AGCAGCAGTG ATGACCGCAT GAATGAGCCT CCCACCTCGC TGGGGCTTGT ACCTCACCAG 
ATCCGGGGCT TTCTGTCACG GTTCGATAAT GTCCTTCCTG TCAGCCTGGC ATTTGATAAA 
TGTACAGCCT GTTCATCCAA AGTTCTTGAT CAATATGAGC AAGAAGGATT CACCTTCCTG 
GCCAAGGTGT TTAACTCTTC ACATTCCTTC TTAGAAGATT TGACTGGTCT TACACTGCTC 
CACCAAGAGA CCCAAGCTGC TGAGATCTGG GACATGAGTG ACGAGGAGAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237095.1
CDS	97..2193
Translation	MGDPGLSKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAFDMSAPTPARCCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGAALENPMLLNKFLLLTFADLKKYHFYYWFCCPALCLPESIPLIRGPVGLDQRLSPKQIQALEHAYDDLCRTEGVTALPYFLFKYDDDTVLVSLLKHYSDFFQGQRTKLTVGVYDPCNLTQHPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAAERLQKIFPGVNASGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEELIDSHDVIFLLMDTRESRWLPTVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEQEGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDEETV

Target ORF information:

RefSeq Version	XM_006237033.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy related 7 (Atg7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237033.3

ORF Insert Sequence:

ATGGGGGACC CTGGACTGTC CAAGCTGCAG TTTGCCCCCT TTAATAGTGC CCTGGACGTT 
GGCTTCTGGC ACGAACTGAC CCAGAAGAAG TTGAACGAGT ATCGCCTGGA CGAGGCACCC 
AAAGACATCA AGGGCTATTA CTACAATGGT GACTCTGCCG GCCTCCCCAC CCGCTTGACA 
TTGGAGTTCA GTGCTTTTGA CATGAGCGCC CCCACGCCTG CCCGCTGCTG CCCGGCCATG 
GGAACCCTGC ACAACACCAA CACACTTGAG GCTTTTAAGA CTGCAGACAA GAAGCTCCTT 
CTGGAGCAGT CAGCCAATGA GATCTGGGAA GCCATAAAGT CAGGTGCTGC TCTCGAAAAC 
CCCATGCTCC TCAACAAGTT TCTGCTCTTG ACCTTCGCGG ACCTAAAGAA GTACCACTTC 
TACTACTGGT TTTGCTGCCC TGCCCTCTGT CTTCCTGAGA GCATCCCTCT AATCCGGGGA 
CCTGTGGGCT TGGATCAAAG GCTTTCACCA AAACAGATCC AGGCCCTGGA GCATGCCTAC 
GATGACCTGT GTCGAACTGA AGGCGTCACA GCCCTGCCAT ACTTCTTATT CAAGTACGAT 
GACGACACTG TGCTGGTCTC CTTGCTCAAA CACTACAGTG ATTTCTTCCA AGGTCAAAGG 
ACAAAGTTAA CAGTCGGTGT GTACGATCCC TGTAACCTAA CCCAGCACCC TGGATGGCCT 
TTGAGGAATT TTTTGGTCCT GGCAGCCCAC AGATGGAGCG GCAGTTTCCA GTCTGTTGAA 
GTTCTCTGCT TTCGGGACCG CACCATGCAG GGAGCAAGAG ATGTGACACA CAGCATCATC 
TTTGAAGTGA AACTTCCAGA AATGGCGTTT AGCCCAGATT GTCCTAAGGC AGTTGGCTGG 
GAGAAGAACC AGAAAGGAGG CATGGGTCCG AGGATGGTGA ACCTCAGCGG ATGTATGGAC 
CCCAAAAGGC TGGCTGAGTC ATCCGTGGAT CTGAATCTCA AGCTGATGTG CTGGCGGTTG 
GTCCCCACCT TGGACTTGGA CAAGGTCGTG TCTGTCAAGT GCCTGCTGCT GGGAGCTGGT 
ACCTTGGGGT GTAATGTGGC TAGAACACTG ATGGGCTGGG GCGTCAGACA CGTCACGTTT 
GTGGACAACG CCAAGATCTC CTACTCCAAT CCCGTGAGGC AGCCTCTGTA TGAATTTGAA 
GATTGTCTAG GGGGTGGCAA GCCCAAGGCC CTGGCTGCAG CAGAGCGGCT CCAGAAAATA 
TTTCCCGGAG TGAATGCCAG CGGGTTCAAC ATGAGCATCC CCATGCCCGG ACACCCTGTG 
AACTTCTCTG ACGTCACGAT GGAGCAGGCC CGCAGAGATG TGGAGCAGCT GGAGGAACTC 
ATCGATAGCC ACGATGTCAT CTTCCTGCTA ATGGACACCA GGGAGAGCCG ATGGCTTCCT 
ACTGTTATTG CAGCCAGCAA GCGAAAGCTG GTCATCAATG CTGCCTTGGG GTTTGACACC 
TTTGTTGTCA TGAGACATGG CCTGAAGAAA CCCAAGCAGC AGGGAGCCGG GGACCTGTGC 
CCAAGCCATC TTGTAGCACC TGCTGACCTG GGCTCCTCAC TTTTTGCCAA CATCCCTGGA 
TACAAGCTTG GCTGCTACTT CTGCAATGAT GTGGTGGCTC CAGGAGATTC CACCAGAGAC 
CGGACTCTGG ACCAGCAGTG CACAGTGAGC CGCCCAGGGC TGGCCGTGAT TGCAGGAGCC 
CTGGCTGTGG AGCTGATGGT TTCTGTCTTG CAGCATCCTG AGGGGGGCTA CGCCATCGCC 
AGCAGCAGTG ATGACCGCAT GAATGAGCCT CCCACCTCGC TGGGGCTTGT ACCTCACCAG 
ATCCGGGGCT TTCTGTCACG GTTCGATAAT GTCCTTCCTG TCAGCCTGGC ATTTGATAAA 
TGTACAGCCT GTTCATCCAA AGTTCTTGAT CAATATGAGC AAGAAGGATT CACCTTCCTG 
GCCAAGGTGT TTAACTCTTC ACATTCCTTC TTAGAAGATT TGACTGGTCT TACACTGCTC 
CACCAAGAGA CCCAAGCTGC TGAGATCTGG GACATGAGTG ACGAGGAGAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg7 ( XM_006237034.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237096.1 Rattus norvegicus autophagy related 7 (Atg7), transcript variant X3, mRNA.

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CloneID	ORa27566
Clone ID Related Accession (Same CDS sequence)	XM_006237034.3
Accession Version	XM_006237034.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme ATG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACC CTGGACTGTC CAAGCTGCAG TTTGCCCCCT TTAATAGTGC CCTGGACGTT 
GGCTTCTGGC ACGAACTGAC CCAGAAGAAG TTGAACGAGT ATCGCCTGGA CGAGGCACCC 
AAAGACATCA AGGGCTATTA CTACAATGGT GACTCTGCCG GCCTCCCCAC CCGCTTGACA 
TTGGAGTTCA GTGCTTTTGA CATGAGCGCC CCCACGCCTG CCCGCTGCTG CCCGGCCATG 
GGAACCCTGC ACAACACCAA CACACTTGAG GCTTTTAAGA CTGCAGACAA GAAGCTCCTT 
CTGGAGCAGT CAGCCAATGA GATCTGGGAA GCCATAAAGT CAGGTGCTGC TCTCGAAAAC 
CCCATGCTCC TCAACAAGTT TCTGCTCTTG ACCTTCGCGG ACCTAAAGAA GTACCACTTC 
TACTACTGGT TTTGCTGCCC TGCCCTCTGT CTTCCTGAGA GCATCCCTCT AATCCGGGGA 
CCTGTGGGCT TGGATCAAAG GCTTTCACCA AAACAGATCC AGGCCCTGGA GCATGCCTAC 
GATGACCTGT GTCGAACTGA AGGCGTCACA GCCCTGCCAT ACTTCTTATT CAAGTACGAT 
GACGACACTG TGCTGGTCTC CTTGCTCAAA CACTACAGTG ATTTCTTCCA AGGTCAAAGG 
ACAAAGTTAA CAGTCGGTGT GTACGATCCC TGTAACCTAA CCCAGCACCC TGGATGGCCT 
TTGAGGAATT TTTTGGTCCT GGCAGCCCAC AGATGGAGCG GCAGTTTCCA GTCTGTTGAA 
GTTCTCTGCT TTCGGGACCG CACCATGCAG GGAGCAAGAG ATGTGACACA CAGCATCATC 
TTTGAAGTGA AACTTCCAGA AATGGCGTTT AGCCCAGATT GTCCTAAGGC AGTTGGCTGG 
GAGAAGAACC AGAAAGGAGG CATGGGTCCG AGGATGGTGA ACCTCAGCGG ATGTATGGAC 
CCCAAAAGGC TGGCTGAGTC ATCCGTGGAT CTGAATCTCA AGCTGATGTG CTGGCGGTTG 
GTCCCCACCT TGGACTTGGA CAAGGTCGTG TCTGTCAAGT GCCTGCTGCT GGGAGCTGGT 
ACCTTGGGGT GTAATGTGGC TAGAACACTG ATGGGCTGGG GCGTCAGACA CGTCACGTTT 
GTGGACAACG CCAAGATCTC CTACTCCAAT CCCGTGAGGC AGCCTCTGTA TGAATTTGAA 
GATTGTCTAG GGGGTGGCAA GCCCAAGGCC CTGGCTGCAG CAGAGCGGCT CCAGAAAATA 
TTTCCCGGAG TGAATGCCAG CGGGTTCAAC ATGAGCATCC CCATGCCCGG ACACCCTGTG 
AACTTCTCTG ACGTCACGAT GGAGCAGGCC CGCAGAGATG TGGAGCAGCT GGAGGAACTC 
ATCGATAGCC ACGATGTCAT CTTCCTGCTA ATGGACACCA GGGAGAGCCG ATGGCTTCCT 
ACTGTTATTG CAGCCAGCAA GCGAAAGCTG GTCATCAATG CTGCCTTGGG GTTTGACACC 
TTTGTTGTCA TGAGACATGG CCTGAAGAAA CCCAAGCAGC AGGGAGCCGG GGACCTGTGC 
CCAAGCCATC TTGTAGCACC TGCTGACCTG GGCTCCTCAC TTTTTGCCAA CATCCCTGGA 
TACAAGCTTG GCTGCTACTT CTGCAATGAT GTGGTGGCTC CAGGAGATTC CACCAGAGAC 
CGGACTCTGG ACCAGCAGTG CACAGTGAGC CGCCCAGGGC TGGCCGTGAT TGCAGGAGCC 
CTGGCTGTGG AGCTGATGGT TTCTGTCTTG CAGCATCCTG AGGGGGGCTA CGCCATCGCC 
AGCAGCAGTG ATGACCGCAT GAATGAGCCT CCCACCTCGC TGGGGCTTGT ACCTCACCAG 
ATCCGGGGCT TTCTGTCACG GTTCGATAAT GTCCTTCCTG TCAGCCTGGC ATTTGATAAA 
TGTACAGCCT GTTCATCCAA AGTTCTTGAT CAATATGAGC AAGAAGGATT CACCTTCCTG 
GCCAAGGTGT TTAACTCTTC ACATTCCTTC TTAGAAGATT TGACTGGTCT TACACTGCTC 
CACCAAGAGA CCCAAGCTGC TGAGGCCTTC AACTGGCTCA TGACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237096.1
CDS	97..2184
Translation	MGDPGLSKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAFDMSAPTPARCCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGAALENPMLLNKFLLLTFADLKKYHFYYWFCCPALCLPESIPLIRGPVGLDQRLSPKQIQALEHAYDDLCRTEGVTALPYFLFKYDDDTVLVSLLKHYSDFFQGQRTKLTVGVYDPCNLTQHPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAAERLQKIFPGVNASGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEELIDSHDVIFLLMDTRESRWLPTVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEQEGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEAFNWLMT

Target ORF information:

RefSeq Version	XM_006237034.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy related 7 (Atg7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237034.3

ORF Insert Sequence:

ATGGGGGACC CTGGACTGTC CAAGCTGCAG TTTGCCCCCT TTAATAGTGC CCTGGACGTT 
GGCTTCTGGC ACGAACTGAC CCAGAAGAAG TTGAACGAGT ATCGCCTGGA CGAGGCACCC 
AAAGACATCA AGGGCTATTA CTACAATGGT GACTCTGCCG GCCTCCCCAC CCGCTTGACA 
TTGGAGTTCA GTGCTTTTGA CATGAGCGCC CCCACGCCTG CCCGCTGCTG CCCGGCCATG 
GGAACCCTGC ACAACACCAA CACACTTGAG GCTTTTAAGA CTGCAGACAA GAAGCTCCTT 
CTGGAGCAGT CAGCCAATGA GATCTGGGAA GCCATAAAGT CAGGTGCTGC TCTCGAAAAC 
CCCATGCTCC TCAACAAGTT TCTGCTCTTG ACCTTCGCGG ACCTAAAGAA GTACCACTTC 
TACTACTGGT TTTGCTGCCC TGCCCTCTGT CTTCCTGAGA GCATCCCTCT AATCCGGGGA 
CCTGTGGGCT TGGATCAAAG GCTTTCACCA AAACAGATCC AGGCCCTGGA GCATGCCTAC 
GATGACCTGT GTCGAACTGA AGGCGTCACA GCCCTGCCAT ACTTCTTATT CAAGTACGAT 
GACGACACTG TGCTGGTCTC CTTGCTCAAA CACTACAGTG ATTTCTTCCA AGGTCAAAGG 
ACAAAGTTAA CAGTCGGTGT GTACGATCCC TGTAACCTAA CCCAGCACCC TGGATGGCCT 
TTGAGGAATT TTTTGGTCCT GGCAGCCCAC AGATGGAGCG GCAGTTTCCA GTCTGTTGAA 
GTTCTCTGCT TTCGGGACCG CACCATGCAG GGAGCAAGAG ATGTGACACA CAGCATCATC 
TTTGAAGTGA AACTTCCAGA AATGGCGTTT AGCCCAGATT GTCCTAAGGC AGTTGGCTGG 
GAGAAGAACC AGAAAGGAGG CATGGGTCCG AGGATGGTGA ACCTCAGCGG ATGTATGGAC 
CCCAAAAGGC TGGCTGAGTC ATCCGTGGAT CTGAATCTCA AGCTGATGTG CTGGCGGTTG 
GTCCCCACCT TGGACTTGGA CAAGGTCGTG TCTGTCAAGT GCCTGCTGCT GGGAGCTGGT 
ACCTTGGGGT GTAATGTGGC TAGAACACTG ATGGGCTGGG GCGTCAGACA CGTCACGTTT 
GTGGACAACG CCAAGATCTC CTACTCCAAT CCCGTGAGGC AGCCTCTGTA TGAATTTGAA 
GATTGTCTAG GGGGTGGCAA GCCCAAGGCC CTGGCTGCAG CAGAGCGGCT CCAGAAAATA 
TTTCCCGGAG TGAATGCCAG CGGGTTCAAC ATGAGCATCC CCATGCCCGG ACACCCTGTG 
AACTTCTCTG ACGTCACGAT GGAGCAGGCC CGCAGAGATG TGGAGCAGCT GGAGGAACTC 
ATCGATAGCC ACGATGTCAT CTTCCTGCTA ATGGACACCA GGGAGAGCCG ATGGCTTCCT 
ACTGTTATTG CAGCCAGCAA GCGAAAGCTG GTCATCAATG CTGCCTTGGG GTTTGACACC 
TTTGTTGTCA TGAGACATGG CCTGAAGAAA CCCAAGCAGC AGGGAGCCGG GGACCTGTGC 
CCAAGCCATC TTGTAGCACC TGCTGACCTG GGCTCCTCAC TTTTTGCCAA CATCCCTGGA 
TACAAGCTTG GCTGCTACTT CTGCAATGAT GTGGTGGCTC CAGGAGATTC CACCAGAGAC 
CGGACTCTGG ACCAGCAGTG CACAGTGAGC CGCCCAGGGC TGGCCGTGAT TGCAGGAGCC 
CTGGCTGTGG AGCTGATGGT TTCTGTCTTG CAGCATCCTG AGGGGGGCTA CGCCATCGCC 
AGCAGCAGTG ATGACCGCAT GAATGAGCCT CCCACCTCGC TGGGGCTTGT ACCTCACCAG 
ATCCGGGGCT TTCTGTCACG GTTCGATAAT GTCCTTCCTG TCAGCCTGGC ATTTGATAAA 
TGTACAGCCT GTTCATCCAA AGTTCTTGAT CAATATGAGC AAGAAGGATT CACCTTCCTG 
GCCAAGGTGT TTAACTCTTC ACATTCCTTC TTAGAAGATT TGACTGGTCT TACACTGCTC 
CACCAAGAGA CCCAAGCTGC TGAGGCCTTC AACTGGCTCA TGACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg7 ( XM_008763239.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761461.1 Rattus norvegicus autophagy related 7 (Atg7), transcript variant X1, mRNA.

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CloneID	ORa47146
Clone ID Related Accession (Same CDS sequence)	XM_008763239.2
Accession Version	XM_008763239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme ATG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACC CTGGACTGTC CAAGCTGCAG TTTGCCCCCT TTAATAGTGC CCTGGACGTT 
GGCTTCTGGC ACGAACTGAC CCAGAAGAAG TTGAACGAGT ATCGCCTGGA CGAGGCACCC 
AAAGACATCA AGGGCTATTA CTACAATGGT GACTCTGCCG GCCTCCCCAC CCGCTTGACA 
TTGGAGTTCA GTGCTTTTGA CATGAGCGCC CCCACGCCTG CCCGCTGCTG CCCGGCCATG 
GGAACCCTGC ACAACACCAA CACACTTGAG GCTTTTAAGA CTGCAGACAA GAAGCTCCTT 
CTGGAGCAGT CAGCCAATGA GATCTGGGAA GCCATAAAGT CAGGTGCTGC TCTCGAAAAC 
CCCATGCTCC TCAACAAGTT TCTGCTCTTG ACCTTCGCGG ACCTAAAGAA GTACCACTTC 
TACTACTGGT TTTGCTGCCC TGCCCTCTGT CTTCCTGAGA GCATCCCTCT AATCCGGGGA 
CCTGTGGGCT TGGATCAAAG GCTTTCACCA AAACAGATCC AGGCCCTGGA GCATGCCTAC 
GATGACCTGT GTCGAACTGA AGGCGTCACA GCCCTGCCAT ACTTCTTATT CAAGTACGAT 
GACGACACTG TGCTGGTCTC CTTGCTCAAA CACTACAGTG ATTTCTTCCA AGGTCAAAGG 
ACAAAGTTAA CAGTCGGTGT GTACGATCCC TGTAACCTAA CCCAGCACCC TGGATGGCCT 
TTGAGGAATT TTTTGGTCCT GGCAGCCCAC AGATGGAGCG GCAGTTTCCA GTCTGTTGAA 
GTTCTCTGCT TTCGGGACCG CACCATGCAG GGAGCAAGAG ATGTGACACA CAGCATCATC 
TTTGAAGTGA AACTTCCAGA AATGGCGTTT AGCCCAGATT GTCCTAAGGC AGTTGGCTGG 
GAGAAGAACC AGAAAGGAGG CATGGGTCCG AGGATGGTGA ACCTCAGCGG ATGTATGGAC 
CCCAAAAGGC TGGCTGAGTC ATCCGTGGAT CTGAATCTCA AGCTGATGTG CTGGCGGTTG 
GTCCCCACCT TGGACTTGGA CAAGGTCGTG TCTGTCAAGT GCCTGCTGCT GGGAGCTGGT 
ACCTTGGGGT GTAATGTGGC TAGAACACTG ATGGGCTGGG GCGTCAGACA CGTCACGTTT 
GTGGACAACG CCAAGATCTC CTACTCCAAT CCCGTGAGGC AGCCTCTGTA TGAATTTGAA 
GATTGTCTAG GGGGTGGCAA GCCCAAGGCC CTGGCTGCAG CAGAGCGGCT CCAGAAAATA 
TTTCCCGGAG TGAATGCCAG CGGGTTCAAC ATGAGCATCC CCATGCCCGG ACACCCTGTG 
AACTTCTCTG ACGTCACGAT GGAGCAGGCC CGCAGAGATG TGGAGCAGCT GGAGGAACTC 
ATCGATAGCC ACGATGTCAT CTTCCTGCTA ATGGACACCA GGGAGAGCCG ATGGCTTCCT 
ACTGTTATTG CAGCCAGCAA GCGAAAGCTG GTCATCAATG CTGCCTTGGG GTTTGACACC 
TTTGTTGTCA TGAGACATGG CCTGAAGAAA CCCAAGCAGC AGGGAGCCGG GGACCTGTGC 
CCAAGCCATC TTGTAGCACC TGCTGACCTG GGCTCCTCAC TTTTTGCCAA CATCCCTGGA 
TACAAGCTTG GCTGCTACTT CTGCAATGAT GTGGTGGCTC CAGGAGATTC CACCAGAGAC 
CGGACTCTGG ACCAGCAGTG CACAGTGAGC CGCCCAGGGC TGGCCGTGAT TGCAGGAGCC 
CTGGCTGTGG AGCTGATGGT TTCTGTCTTG CAGCATCCTG AGGGGGGCTA CGCCATCGCC 
AGCAGCAGTG ATGACCGCAT GAATGAGCCT CCCACCTCGC TGGGGCTTGT ACCTCACCAG 
ATCCGGGGCT TTCTGTCACG GTTCGATAAT GTCCTTCCTG TCAGCCTGGC ATTTGATAAA 
TGTACAGCCT GTTCATCCAA AGTTCTTGAT CAATATGAGC AAGAAGGATT CACCTTCCTG 
GCCAAGGTGT TTAACTCTTC ACATTCCTTC TTAGAAGATT TGACTGGTCT TACACTGCTC 
CACCAAGAGA CCCAAGCTGC TGAGGGGCTT TTCAAGATGG AGTCTGTGAG GCAGAGCCTG 
GGAACTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761461.1
CDS	97..2208
Translation	MGDPGLSKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAFDMSAPTPARCCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGAALENPMLLNKFLLLTFADLKKYHFYYWFCCPALCLPESIPLIRGPVGLDQRLSPKQIQALEHAYDDLCRTEGVTALPYFLFKYDDDTVLVSLLKHYSDFFQGQRTKLTVGVYDPCNLTQHPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAAERLQKIFPGVNASGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEELIDSHDVIFLLMDTRESRWLPTVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEQEGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEGLFKMESVRQSLGTE

Target ORF information:

RefSeq Version	XM_008763239.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy related 7 (Atg7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763239.2

ORF Insert Sequence:

ATGGGGGACC CTGGACTGTC CAAGCTGCAG TTTGCCCCCT TTAATAGTGC CCTGGACGTT 
GGCTTCTGGC ACGAACTGAC CCAGAAGAAG TTGAACGAGT ATCGCCTGGA CGAGGCACCC 
AAAGACATCA AGGGCTATTA CTACAATGGT GACTCTGCCG GCCTCCCCAC CCGCTTGACA 
TTGGAGTTCA GTGCTTTTGA CATGAGCGCC CCCACGCCTG CCCGCTGCTG CCCGGCCATG 
GGAACCCTGC ACAACACCAA CACACTTGAG GCTTTTAAGA CTGCAGACAA GAAGCTCCTT 
CTGGAGCAGT CAGCCAATGA GATCTGGGAA GCCATAAAGT CAGGTGCTGC TCTCGAAAAC 
CCCATGCTCC TCAACAAGTT TCTGCTCTTG ACCTTCGCGG ACCTAAAGAA GTACCACTTC 
TACTACTGGT TTTGCTGCCC TGCCCTCTGT CTTCCTGAGA GCATCCCTCT AATCCGGGGA 
CCTGTGGGCT TGGATCAAAG GCTTTCACCA AAACAGATCC AGGCCCTGGA GCATGCCTAC 
GATGACCTGT GTCGAACTGA AGGCGTCACA GCCCTGCCAT ACTTCTTATT CAAGTACGAT 
GACGACACTG TGCTGGTCTC CTTGCTCAAA CACTACAGTG ATTTCTTCCA AGGTCAAAGG 
ACAAAGTTAA CAGTCGGTGT GTACGATCCC TGTAACCTAA CCCAGCACCC TGGATGGCCT 
TTGAGGAATT TTTTGGTCCT GGCAGCCCAC AGATGGAGCG GCAGTTTCCA GTCTGTTGAA 
GTTCTCTGCT TTCGGGACCG CACCATGCAG GGAGCAAGAG ATGTGACACA CAGCATCATC 
TTTGAAGTGA AACTTCCAGA AATGGCGTTT AGCCCAGATT GTCCTAAGGC AGTTGGCTGG 
GAGAAGAACC AGAAAGGAGG CATGGGTCCG AGGATGGTGA ACCTCAGCGG ATGTATGGAC 
CCCAAAAGGC TGGCTGAGTC ATCCGTGGAT CTGAATCTCA AGCTGATGTG CTGGCGGTTG 
GTCCCCACCT TGGACTTGGA CAAGGTCGTG TCTGTCAAGT GCCTGCTGCT GGGAGCTGGT 
ACCTTGGGGT GTAATGTGGC TAGAACACTG ATGGGCTGGG GCGTCAGACA CGTCACGTTT 
GTGGACAACG CCAAGATCTC CTACTCCAAT CCCGTGAGGC AGCCTCTGTA TGAATTTGAA 
GATTGTCTAG GGGGTGGCAA GCCCAAGGCC CTGGCTGCAG CAGAGCGGCT CCAGAAAATA 
TTTCCCGGAG TGAATGCCAG CGGGTTCAAC ATGAGCATCC CCATGCCCGG ACACCCTGTG 
AACTTCTCTG ACGTCACGAT GGAGCAGGCC CGCAGAGATG TGGAGCAGCT GGAGGAACTC 
ATCGATAGCC ACGATGTCAT CTTCCTGCTA ATGGACACCA GGGAGAGCCG ATGGCTTCCT 
ACTGTTATTG CAGCCAGCAA GCGAAAGCTG GTCATCAATG CTGCCTTGGG GTTTGACACC 
TTTGTTGTCA TGAGACATGG CCTGAAGAAA CCCAAGCAGC AGGGAGCCGG GGACCTGTGC 
CCAAGCCATC TTGTAGCACC TGCTGACCTG GGCTCCTCAC TTTTTGCCAA CATCCCTGGA 
TACAAGCTTG GCTGCTACTT CTGCAATGAT GTGGTGGCTC CAGGAGATTC CACCAGAGAC 
CGGACTCTGG ACCAGCAGTG CACAGTGAGC CGCCCAGGGC TGGCCGTGAT TGCAGGAGCC 
CTGGCTGTGG AGCTGATGGT TTCTGTCTTG CAGCATCCTG AGGGGGGCTA CGCCATCGCC 
AGCAGCAGTG ATGACCGCAT GAATGAGCCT CCCACCTCGC TGGGGCTTGT ACCTCACCAG 
ATCCGGGGCT TTCTGTCACG GTTCGATAAT GTCCTTCCTG TCAGCCTGGC ATTTGATAAA 
TGTACAGCCT GTTCATCCAA AGTTCTTGAT CAATATGAGC AAGAAGGATT CACCTTCCTG 
GCCAAGGTGT TTAACTCTTC ACATTCCTTC TTAGAAGATT TGACTGGTCT TACACTGCTC 
CACCAAGAGA CCCAAGCTGC TGAGGGGCTT TTCAAGATGG AGTCTGTGAG GCAGAGCCTG 
GGAACTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY1.
Cell metabolism28(2)268-281.e4(2018 08)
Toledo M,Batista-Gonzalez A,Merheb E,Aoun ML,Tarabra E,Feng D,Sarparanta J,Merlo P,Botrè F,Schwartz GJ,Pessin JE,Singh R

Oxidation of Atg3 and Atg7 mediates inhibition of autophagy.
Nature communications9(1)95(2018 01)
Frudd K,Burgoyne T,Burgoyne JR

miR-96 attenuates status epilepticus-induced brain injury by directly targeting Atg7 and Atg16L1.
Scientific reports7(1)10270(2017 08)
Gan J,Cai Q,Qu Y,Zhao F,Wan C,Luo R,Mu D

Geldanamycin Reduces Aβ-Associated Anxiety and Depression, Concurrent with Autophagy Provocation.
Journal of molecular neuroscience : MN57(3)317-24(2015 Nov)
Zare N,Khalifeh S,Khodagholi F,Shahamati SZ,Motamedi F,Maghsoudi N

Emerging role for autophagy in the removal of aggresomes in Schwann cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience23(33)10672-80(2003 Nov)
Fortun J,Dunn WA Jr,Joy S,Li J,Notterpek L

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Atg7 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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