Fastkd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fastkd1
Entrez Gene ID	311112
Full Name	FAST kinase domains 1
Synonyms	RGD1563531
General protein information	Preferred Names FAST kinase domains 1 Names FAST kinase domain-containing protein 1, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191738.1	NP_001178667.1	FAST kinase domain-containing protein 1, mitochondrial
XM_017591689.1	XP_017447178.1	FAST kinase domain-containing protein 1, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FASTKD1	NP_078898.3
Pan troglodytes (chimpanzee)	FASTKD1	XP_515885.2
Macaca mulatta (Rhesus monkey)	LOC706154	XP_002808048.1
Canis lupus familiaris (dog)	FASTKD1	XP_005640459.1
Danio rerio (zebrafish)	fastkd1	NP_991192.2
Mus musculus (house mouse)	Fastkd1	NP_796218.2
Gallus gallus (chicken)	FASTKD1	XP_004942825.1
Xenopus tropicalis (tropical clawed frog)	fastkd1	NP_001039188.1
Bos taurus (cattle)	FASTKD1	NP_001095501.1
Rattus norvegicus (Norway rat)	Fastkd1	NP_001178667.1

Gene Ontology
Bibliography

Related articles in PubMed

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.
Boehm E, Zaganelli S, Maundrell K, Jourdain AA, Thore S, Martinou JC
Nucleic acids research45(10)6135-6146(2017 Jun)

Proximity Biotinylation as a Method for Mapping Proteins Associated with mtDNA in Living Cells.
Han S, Udeshi ND, Deerinck TJ, Svinkina T, Ellisman MH, Carr SA, Ting AY
Cell chemical biology24(3)404-414(2017 Mar)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration.
Simarro M, Gimenez-Cassina A, Kedersha N, Lazaro JB, Adelmant GO, Marto JA, Rhee K, Tisdale S, Danial N, Benarafa C, Orduña A, Anderson P
Biochemical and biophysical research communications401(3)440-6(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fastkd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fastkd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07525	NM_001191738.1 Latest version!	Rattus norvegicus FAST kinase domains 1 (Fastkd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa25935	XM_017591689.1 Latest version!	Rattus norvegicus FAST kinase domains 1 (Fastkd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fastkd1 ( NM_001191738.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178667.1 Rattus norvegicus FAST kinase domains 1 (Fastkd1), mRNA.

Price & Availability↑

CloneID	ORa07525
Clone ID Related Accession (Same CDS sequence)	NM_001191738.1
Accession Version	NM_001191738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570636800000
Organism	Rattus norvegicus(Norway rat)
Product	FAST kinase domain-containing protein 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07072867.1 and AABR07052473.1. On Jul 17, 2010 this sequence version replaced XM_230003.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGTTCCGTC TACGAGGCGT CTCTCTACTC TCTTGGAGAG CGTTTCCGTT CCGCCCCTTC 
AGTTGTGAGT CATTAATTGT TCAGATGCAG AACTGTGCAG ATGAAGAACA AGTGTTTGAT 
CTTATCGAGA GAAATAAGGC CACGCTCTCA GAACAGCAAG TGGGCTGTGC ATTTAACGTA 
CTTTGGCAGT TTCAGAAGCA GAAGACCATC TTGGAGAAAA ATGTTGAGCG CGTTAGAAAC 
CATCCTCAGT TTCTTAGTCT TTGCAGTGTT ACGACAGATC ACATCCCAGC AATGAGTGAT 
GTCACCCTGG TGGATGTGTT ATACAGCATA AAACAGTTTG CAGTTGAATC CCATCACCCA 
CTAATTGAGG CCCTGGTCAC AGAAGGGTGG CGAAGACTCG AAAGGTTCGA TATTAATGTG 
CTGTCAATAT TTTCCACTTG CCTGGCCGAT CAGCACTTGT ATTTTAGTCC ATTAATGGGA 
AAAATAGCTG ACATTGTGAA TAGGAAACTG GAAGCCATAC AGGACTTAAG GGCCTTGTCT 
GTCTTGATGG TCAGCATTTC TTCTTTAATA TCACCGTGCT TTCAAGAACG ACTGCTGACC 
AGAGCAGAAC TTCTTTTTGA CACTGTAGAT TCTTCCAAGG TCAACATTGC AAGGAGGATA 
CTACTGTTCC TTCGGAATGT CAAGCACAGT CACTACCCAC TGTTAGAGAG GTGCAATCAA 
GTGTTCATCA GAAATGTGAG CCATCTTGAC TTGGAGTCCA TCAGTCGAAT CCTTAGTCTG 
TATCAGTCTC TACAGTTCCA CAGCTTTGAA TTTATCGAGG TGGCTCGAGG GAGGCTGGCT 
GAGCTGAGCC TCCCTTCTGA CCATCCTGAA AGCTTCGTCA GACTGTTTGC TGCGCTGGGG 
CCGGTGGCCA GGCCTGAGAT AAGGAAACAG CTTAAATCAA CTATACTGCT GCTGTCAGAG 
GAGCTGAGCA GCCAGCAAGC GCTGACTGTG TTGGGAGCAA TGGAAGACAT GGAAAGCAGA 
GACTCACACT TGATTAAAAA GATCGTGTCT GTCCTGTACA GACATTTGGA TAACTGTAAA 
TCAGTAGAGC TATTGAAGAT AATACAAGCG CTGACATTCC TACACTTCCA AAGTAAGGAG 
CTTTTTATGA AACTCAGAGA AGTACTTCTC AGTCGTTTGG AAGCTAGTGT CACACCCAGC 
GAGGTTTCCG TTCTGGTCTC TGCTCTCTCC ATGCTTCCAT ATCCTCACCT CAACGAAACA 
GCCCTGTCCC GAACTGAGAC AGTTTTACCA CAGTGCGACT TCAGGGAACT GAGTGATTTG 
GTAGTGTGTC TCATGCGCTG GATTCAGAAT GATCACCTGT GCTCGGCTAG CACTGCTGGG 
AAGCAACTGG ACCTCCTTCA AAAACTGGAT CACTGGGGCC ATCGCCGGCT GCAGCAGAGC 
ACCAGCTTGG ATCTACTGTG GGAGGAGCTT AAGTCTGTGA AGGGAGAGTG GCTTCATGAG 
TCCCTGGTTG AAGAATCAAT CACCGCTTTG CAGCGCTTTA TGGATGAGAT CGATCACAGC 
AATGTCGCCA AGATCGCATC TTTCCTTTCC AAAACTAACT ACCTCAGCAC TCCGCTGCTC 
GACAGGATAG CTTCGGTGGT CGTTGAGCAG ATCAAGAAGA TCCATCCCTT TTCAGTCCTT 
GCTATTATTC TCCCATTCAG CATTTTGAAC TATGATCCAC CTCAAAAGGA TGAATTTTTT 
GGAGCGTGTG TCCAGTGCTG TAATTCTTAC TTAGGTACAT TGGATCCTGG GACATTAGTG 
TTTCTTGGTT TCTCTATGGC TACACTTGAA TATTTTCCAG AAGATCTGCT AAAGAAAATG 
TTTAACATCG AGTTCCTAGC CAGATTAGAT TCCCAGCTTG AAATTTTACC TTCAAGTCTC 
AGTGCAAGGA TCCAGTTCCG CCTTATGGAG TTAAATAGAG CCGTCTGCTT GGAGTGCCCC 
GAGCTGCAGG TCCCGTGGTT TCATGACCGC TTTTGTCAGC GACAGTTTAA CAAAGATACC 
GGTGTTATGA ATGGAGCGCA ACAACAGATC TATAAAATGT TAGCAGAGGT CCTGGGAGGA 
CACCGATATG TAAAGCCTTC GACTCTTTCA CCATATTACC ACACAGTGGA TTTTGAATGC 
ATTTTGGATA AACGAAAAAA GCCCCTGCCT TATGAAAGCC ACAGCGTAAC TCCGGGAAAA 
TCCCCAGGAG TACACGGGGA TTCAAATACT TCAAGAGCTG AGCCAAGGCT TCCACCAGAA 
GCTGAGAGGA TTGCTATAGA ATTTTTGGAT GTAAGAGCCT TTTGTTCAAA CATTCTTCAC 
TTAAAAGGAA AATCTGCTAT GAAAAAGCGG CATTTGGAAA TCTTGGGCTA TCGTGTTATT 
CAGGTCCCCT ACTTTGAATG GAACTCCATG GCAATGTCAA CGAAGGATAC ACGGATGGAC 
TACCTGAGAG AACAGATATT TGGAGCAGGC AGATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178667.1
CDS	1005..3503
Translation	MFRLRGVSLLSWRAFPFRPFSCESLIVQMQNCADEEQVFDLIERNKATLSEQQVGCAFNVLWQFQKQKTILEKNVERVRNHPQFLSLCSVTTDHIPAMSDVTLVDVLYSIKQFAVESHHPLIEALVTEGWRRLERFDINVLSIFSTCLADQHLYFSPLMGKIADIVNRKLEAIQDLRALSVLMVSISSLISPCFQERLLTRAELLFDTVDSSKVNIARRILLFLRNVKHSHYPLLERCNQVFIRNVSHLDLESISRILSLYQSLQFHSFEFIEVARGRLAELSLPSDHPESFVRLFAALGPVARPEIRKQLKSTILLLSEELSSQQALTVLGAMEDMESRDSHLIKKIVSVLYRHLDNCKSVELLKIIQALTFLHFQSKELFMKLREVLLSRLEASVTPSEVSVLVSALSMLPYPHLNETALSRTETVLPQCDFRELSDLVVCLMRWIQNDHLCSASTAGKQLDLLQKLDHWGHRRLQQSTSLDLLWEELKSVKGEWLHESLVEESITALQRFMDEIDHSNVAKIASFLSKTNYLSTPLLDRIASVVVEQIKKIHPFSVLAIILPFSILNYDPPQKDEFFGACVQCCNSYLGTLDPGTLVFLGFSMATLEYFPEDLLKKMFNIEFLARLDSQLEILPSSLSARIQFRLMELNRAVCLECPELQVPWFHDRFCQRQFNKDTGVMNGAQQQIYKMLAEVLGGHRYVKPSTLSPYYHTVDFECILDKRKKPLPYESHSVTPGKSPGVHGDSNTSRAEPRLPPEAERIAIEFLDVRAFCSNILHLKGKSAMKKRHLEILGYRVIQVPYFEWNSMAMSTKDTRMDYLREQIFGAGRS

Target ORF information:

RefSeq Version	NM_001191738.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FAST kinase domains 1 (Fastkd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191738.1

ORF Insert Sequence:

ATGTTCCGTC TACGAGGCGT CTCTCTACTC TCTTGGAGAG CGTTTCCGTT CCGCCCCTTC 
AGTTGTGAGT CATTAATTGT TCAGATGCAG AACTGTGCAG ATGAAGAACA AGTGTTTGAT 
CTTATCGAGA GAAATAAGGC CACGCTCTCA GAACAGCAAG TGGGCTGTGC ATTTAACGTA 
CTTTGGCAGT TTCAGAAGCA GAAGACCATC TTGGAGAAAA ATGTTGAGCG CGTTAGAAAC 
CATCCTCAGT TTCTTAGTCT TTGCAGTGTT ACGACAGATC ACATCCCAGC AATGAGTGAT 
GTCACCCTGG TGGATGTGTT ATACAGCATA AAACAGTTTG CAGTTGAATC CCATCACCCA 
CTAATTGAGG CCCTGGTCAC AGAAGGGTGG CGAAGACTCG AAAGGTTCGA TATTAATGTG 
CTGTCAATAT TTTCCACTTG CCTGGCCGAT CAGCACTTGT ATTTTAGTCC ATTAATGGGA 
AAAATAGCTG ACATTGTGAA TAGGAAACTG GAAGCCATAC AGGACTTAAG GGCCTTGTCT 
GTCTTGATGG TCAGCATTTC TTCTTTAATA TCACCGTGCT TTCAAGAACG ACTGCTGACC 
AGAGCAGAAC TTCTTTTTGA CACTGTAGAT TCTTCCAAGG TCAACATTGC AAGGAGGATA 
CTACTGTTCC TTCGGAATGT CAAGCACAGT CACTACCCAC TGTTAGAGAG GTGCAATCAA 
GTGTTCATCA GAAATGTGAG CCATCTTGAC TTGGAGTCCA TCAGTCGAAT CCTTAGTCTG 
TATCAGTCTC TACAGTTCCA CAGCTTTGAA TTTATCGAGG TGGCTCGAGG GAGGCTGGCT 
GAGCTGAGCC TCCCTTCTGA CCATCCTGAA AGCTTCGTCA GACTGTTTGC TGCGCTGGGG 
CCGGTGGCCA GGCCTGAGAT AAGGAAACAG CTTAAATCAA CTATACTGCT GCTGTCAGAG 
GAGCTGAGCA GCCAGCAAGC GCTGACTGTG TTGGGAGCAA TGGAAGACAT GGAAAGCAGA 
GACTCACACT TGATTAAAAA GATCGTGTCT GTCCTGTACA GACATTTGGA TAACTGTAAA 
TCAGTAGAGC TATTGAAGAT AATACAAGCG CTGACATTCC TACACTTCCA AAGTAAGGAG 
CTTTTTATGA AACTCAGAGA AGTACTTCTC AGTCGTTTGG AAGCTAGTGT CACACCCAGC 
GAGGTTTCCG TTCTGGTCTC TGCTCTCTCC ATGCTTCCAT ATCCTCACCT CAACGAAACA 
GCCCTGTCCC GAACTGAGAC AGTTTTACCA CAGTGCGACT TCAGGGAACT GAGTGATTTG 
GTAGTGTGTC TCATGCGCTG GATTCAGAAT GATCACCTGT GCTCGGCTAG CACTGCTGGG 
AAGCAACTGG ACCTCCTTCA AAAACTGGAT CACTGGGGCC ATCGCCGGCT GCAGCAGAGC 
ACCAGCTTGG ATCTACTGTG GGAGGAGCTT AAGTCTGTGA AGGGAGAGTG GCTTCATGAG 
TCCCTGGTTG AAGAATCAAT CACCGCTTTG CAGCGCTTTA TGGATGAGAT CGATCACAGC 
AATGTCGCCA AGATCGCATC TTTCCTTTCC AAAACTAACT ACCTCAGCAC TCCGCTGCTC 
GACAGGATAG CTTCGGTGGT CGTTGAGCAG ATCAAGAAGA TCCATCCCTT TTCAGTCCTT 
GCTATTATTC TCCCATTCAG CATTTTGAAC TATGATCCAC CTCAAAAGGA TGAATTTTTT 
GGAGCGTGTG TCCAGTGCTG TAATTCTTAC TTAGGTACAT TGGATCCTGG GACATTAGTG 
TTTCTTGGTT TCTCTATGGC TACACTTGAA TATTTTCCAG AAGATCTGCT AAAGAAAATG 
TTTAACATCG AGTTCCTAGC CAGATTAGAT TCCCAGCTTG AAATTTTACC TTCAAGTCTC 
AGTGCAAGGA TCCAGTTCCG CCTTATGGAG TTAAATAGAG CCGTCTGCTT GGAGTGCCCC 
GAGCTGCAGG TCCCGTGGTT TCATGACCGC TTTTGTCAGC GACAGTTTAA CAAAGATACC 
GGTGTTATGA ATGGAGCGCA ACAACAGATC TATAAAATGT TAGCAGAGGT CCTGGGAGGA 
CACCGATATG TAAAGCCTTC GACTCTTTCA CCATATTACC ACACAGTGGA TTTTGAATGC 
ATTTTGGATA AACGAAAAAA GCCCCTGCCT TATGAAAGCC ACAGCGTAAC TCCGGGAAAA 
TCCCCAGGAG TACACGGGGA TTCAAATACT TCAAGAGCTG AGCCAAGGCT TCCACCAGAA 
GCTGAGAGGA TTGCTATAGA ATTTTTGGAT GTAAGAGCCT TTTGTTCAAA CATTCTTCAC 
TTAAAAGGAA AATCTGCTAT GAAAAAGCGG CATTTGGAAA TCTTGGGCTA TCGTGTTATT 
CAGGTCCCCT ACTTTGAATG GAACTCCATG GCAATGTCAA CGAAGGATAC ACGGATGGAC 
TACCTGAGAG AACAGATATT TGGAGCAGGC AGATCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fastkd1 ( XM_017591689.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447178.1 Rattus norvegicus FAST kinase domains 1 (Fastkd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25935
Clone ID Related Accession (Same CDS sequence)	XM_017591689.1
Accession Version	XM_017591689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	FAST kinase domain-containing protein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAA TAGCTGACAT TGTGAATAGG AAACTGGAAG CCATACAGGA CTTAAGGGCC 
TTGTCTGTCT TGATGGTCAG CATTTCTTCT TTAATATCAC CGTGCTTTCA AGAACGACTG 
CTGACCAGAG CAGAACTTCT TTTTGACACT GTAGATTCTT CCAAGGTCAA CATTGCAAGG 
AGGATACTAC TGTTCCTTCG GAATGTCAAG CACAGTCACT ACCCACTGTT AGAGAGGTGC 
AATCAAGTGT TCATCAGAAA TGTGAGCCAT CTTGACTTGG AGTCCATCAG TCGAATCCTT 
AGTCTGTATC AGTCTCTACA GTTCCACAGC TTTGAATTTA TCGAGGTGGC TCGAGGGAGG 
CTGGCTGAGC TGAGCCTCCC TTCTGACCAT CCTGAAAGCT TCGTCAGACT GTTTGCTGCG 
CTGGGGCCGG TGGCCAGGCC TGAGATAAGG AAACAGCTTA AATCAACTAT ACTGCTGCTG 
TCAGAGGAGC TGAGCAGCCA GCAAGCGCTG ACTGTGTTGG GAGCAATGGA AGACATGGAA 
AGCAGAGACT CACACTTGAT TAAAAAGATC GTGTCTGTCC TGTACAGACA TTTGGATAAC 
TGTAAATCAG TAGAGCTATT GAAGATAATA CAAGCGCTGA CATTCCTACA CTTCCAAAGT 
AAGGAGCTTT TTATGAAACT CAGAGAAGTA CTTCTCAGTC GTTTGGAAGC TAGTGTCACA 
CCCAGCGAGG TTTCCGTTCT GGTCTCTGCT CTCTCCATGC TTCCATATCC TCACCTCAAC 
GAAACAGCCC TGTCCCGAAC TGAGACAGTT TTACCACAGT GCGACTTCAG GGAACTGAGT 
GATTTGGTAG TGTGTCTCAT GCGCTGGATT CAGAATGATC ACCTGTGCTC GGCTAGCACT 
GCTGGGAAGC AACTGGACCT CCTTCAAAAA CTGGATCACT GGGGCCATCG CCGGCTGCAG 
CAGAGCACCA GCTTGGATCT ACTGTGGGAG GAGCTTAAGT CTGTGAAGGG AGAGTGGCTT 
CATGAGTCCC TGGTTGAAGA ATCAATCACC GCTTTGCAGC GCTTTATGGA TGAGATCGAT 
CACAGCAATG TCGCCAAGAT CGCATCTTTC CTTTCCAAAA CTAACTACCT CAGCACTCCG 
CTGCTCGACA GGATAGCTTC GGTGGTCGTT GAGCAGATCA AGAAGATCCA TCCCTTTTCA 
GTCCTTGCTA TTATTCTCCC ATTCAGCATT TTGAACTATG ATCCACCTCA AAAGGATGAA 
TTTTTTGGAG CGTGTGTCCA GTGCTGTAAT TCTTACTTAG GTACATTGGA TCCTGGGACA 
TTAGTGTTTC TTGGTTTCTC TATGGCTACA CTTGAATATT TTCCAGAAGA TCTGCTAAAG 
AAAATGTTTA ACATCGAGTT CCTAGCCAGA TTAGATTCCC AGCTTGAAAT TTTACCTTCA 
AGTCTCAGTG CAAGGATCCA GTTCCGCCTT ATGGAGTTAA ATAGAGCCGT CTGCTTGGAG 
TGCCCCGAGC TGCAGGTCCC GTGGTTTCAT GACCGCTTTT GTCAGCGACA GTTTAACAAA 
GATACCGGTG TTATGAATGG AGCGCAACAA CAGATCTATA AAATGTTAGC AGAGGTCCTG 
GGAGGACACC GATATGTAAA GCCTTCGACT CTTTCACCAT ATTACCACAC AGTGGATTTT 
GAATGCATTT TGGATAAACG AAAAAAGCCC CTGCCTTATG AAAGCCACAG CGTAACTCCG 
GGAAAATCCC CAGGAGTACA CGGGGATTCA AATACTTCAA GAGCTGAGCC AAGGCTTCCA 
CCAGAAGCTG AGAGGATTGC TATAGAATTT TTGGATGTAA GAGCCTTTTG TTCAAACATT 
CTTCACTTAA AAGGAAAATC TGCTATGAAA AAGCGGCATT TGGAAATCTT GGGCTATCGT 
GTTATTCAGG TCCCCTACTT TGAATGGAAC TCCATGGCAA TGTCAACGAA GGATACACGG 
ATGGACTACC TGAGAGAACA GATATTTGGA GCAGGCAGAT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447178.1
CDS	218..2242
Translation	MGKIADIVNRKLEAIQDLRALSVLMVSISSLISPCFQERLLTRAELLFDTVDSSKVNIARRILLFLRNVKHSHYPLLERCNQVFIRNVSHLDLESISRILSLYQSLQFHSFEFIEVARGRLAELSLPSDHPESFVRLFAALGPVARPEIRKQLKSTILLLSEELSSQQALTVLGAMEDMESRDSHLIKKIVSVLYRHLDNCKSVELLKIIQALTFLHFQSKELFMKLREVLLSRLEASVTPSEVSVLVSALSMLPYPHLNETALSRTETVLPQCDFRELSDLVVCLMRWIQNDHLCSASTAGKQLDLLQKLDHWGHRRLQQSTSLDLLWEELKSVKGEWLHESLVEESITALQRFMDEIDHSNVAKIASFLSKTNYLSTPLLDRIASVVVEQIKKIHPFSVLAIILPFSILNYDPPQKDEFFGACVQCCNSYLGTLDPGTLVFLGFSMATLEYFPEDLLKKMFNIEFLARLDSQLEILPSSLSARIQFRLMELNRAVCLECPELQVPWFHDRFCQRQFNKDTGVMNGAQQQIYKMLAEVLGGHRYVKPSTLSPYYHTVDFECILDKRKKPLPYESHSVTPGKSPGVHGDSNTSRAEPRLPPEAERIAIEFLDVRAFCSNILHLKGKSAMKKRHLEILGYRVIQVPYFEWNSMAMSTKDTRMDYLREQIFGAGRS

Target ORF information:

RefSeq Version	XM_017591689.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FAST kinase domains 1 (Fastkd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591689.1

ORF Insert Sequence:

ATGGGAAAAA TAGCTGACAT TGTGAATAGG AAACTGGAAG CCATACAGGA CTTAAGGGCC 
TTGTCTGTCT TGATGGTCAG CATTTCTTCT TTAATATCAC CGTGCTTTCA AGAACGACTG 
CTGACCAGAG CAGAACTTCT TTTTGACACT GTAGATTCTT CCAAGGTCAA CATTGCAAGG 
AGGATACTAC TGTTCCTTCG GAATGTCAAG CACAGTCACT ACCCACTGTT AGAGAGGTGC 
AATCAAGTGT TCATCAGAAA TGTGAGCCAT CTTGACTTGG AGTCCATCAG TCGAATCCTT 
AGTCTGTATC AGTCTCTACA GTTCCACAGC TTTGAATTTA TCGAGGTGGC TCGAGGGAGG 
CTGGCTGAGC TGAGCCTCCC TTCTGACCAT CCTGAAAGCT TCGTCAGACT GTTTGCTGCG 
CTGGGGCCGG TGGCCAGGCC TGAGATAAGG AAACAGCTTA AATCAACTAT ACTGCTGCTG 
TCAGAGGAGC TGAGCAGCCA GCAAGCGCTG ACTGTGTTGG GAGCAATGGA AGACATGGAA 
AGCAGAGACT CACACTTGAT TAAAAAGATC GTGTCTGTCC TGTACAGACA TTTGGATAAC 
TGTAAATCAG TAGAGCTATT GAAGATAATA CAAGCGCTGA CATTCCTACA CTTCCAAAGT 
AAGGAGCTTT TTATGAAACT CAGAGAAGTA CTTCTCAGTC GTTTGGAAGC TAGTGTCACA 
CCCAGCGAGG TTTCCGTTCT GGTCTCTGCT CTCTCCATGC TTCCATATCC TCACCTCAAC 
GAAACAGCCC TGTCCCGAAC TGAGACAGTT TTACCACAGT GCGACTTCAG GGAACTGAGT 
GATTTGGTAG TGTGTCTCAT GCGCTGGATT CAGAATGATC ACCTGTGCTC GGCTAGCACT 
GCTGGGAAGC AACTGGACCT CCTTCAAAAA CTGGATCACT GGGGCCATCG CCGGCTGCAG 
CAGAGCACCA GCTTGGATCT ACTGTGGGAG GAGCTTAAGT CTGTGAAGGG AGAGTGGCTT 
CATGAGTCCC TGGTTGAAGA ATCAATCACC GCTTTGCAGC GCTTTATGGA TGAGATCGAT 
CACAGCAATG TCGCCAAGAT CGCATCTTTC CTTTCCAAAA CTAACTACCT CAGCACTCCG 
CTGCTCGACA GGATAGCTTC GGTGGTCGTT GAGCAGATCA AGAAGATCCA TCCCTTTTCA 
GTCCTTGCTA TTATTCTCCC ATTCAGCATT TTGAACTATG ATCCACCTCA AAAGGATGAA 
TTTTTTGGAG CGTGTGTCCA GTGCTGTAAT TCTTACTTAG GTACATTGGA TCCTGGGACA 
TTAGTGTTTC TTGGTTTCTC TATGGCTACA CTTGAATATT TTCCAGAAGA TCTGCTAAAG 
AAAATGTTTA ACATCGAGTT CCTAGCCAGA TTAGATTCCC AGCTTGAAAT TTTACCTTCA 
AGTCTCAGTG CAAGGATCCA GTTCCGCCTT ATGGAGTTAA ATAGAGCCGT CTGCTTGGAG 
TGCCCCGAGC TGCAGGTCCC GTGGTTTCAT GACCGCTTTT GTCAGCGACA GTTTAACAAA 
GATACCGGTG TTATGAATGG AGCGCAACAA CAGATCTATA AAATGTTAGC AGAGGTCCTG 
GGAGGACACC GATATGTAAA GCCTTCGACT CTTTCACCAT ATTACCACAC AGTGGATTTT 
GAATGCATTT TGGATAAACG AAAAAAGCCC CTGCCTTATG AAAGCCACAG CGTAACTCCG 
GGAAAATCCC CAGGAGTACA CGGGGATTCA AATACTTCAA GAGCTGAGCC AAGGCTTCCA 
CCAGAAGCTG AGAGGATTGC TATAGAATTT TTGGATGTAA GAGCCTTTTG TTCAAACATT 
CTTCACTTAA AAGGAAAATC TGCTATGAAA AAGCGGCATT TGGAAATCTT GGGCTATCGT 
GTTATTCAGG TCCCCTACTT TGAATGGAAC TCCATGGCAA TGTCAACGAA GGATACACGG 
ATGGACTACC TGAGAGAACA GATATTTGGA GCAGGCAGAT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.
Nucleic acids research45(10)6135-6146(2017 Jun)
Boehm E,Zaganelli S,Maundrell K,Jourdain AA,Thore S,Martinou JC

Proximity Biotinylation as a Method for Mapping Proteins Associated with mtDNA in Living Cells.
Cell chemical biology24(3)404-414(2017 Mar)
Han S,Udeshi ND,Deerinck TJ,Svinkina T,Ellisman MH,Carr SA,Ting AY

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration.
Biochemical and biophysical research communications401(3)440-6(2010 Oct)
Simarro M,Gimenez-Cassina A,Kedersha N,Lazaro JB,Adelmant GO,Marto JA,Rhee K,Tisdale S,Danial N,Benarafa C,Orduña A,Anderson P

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Fastkd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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