Fuk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fuk
Entrez Gene ID	307848
Full Name	fucokinase
General protein information	Preferred Names fucokinase Names L-fucose kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107429.1	NP_001100899.1	L-fucose kinase
XM_006255624.3	XP_006255686.2	L-fucose kinase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FUK	XP_003952928.1
Canis lupus familiaris (dog)	FUK	XP_546835.4
Mus musculus (house mouse)	Fuk	NP_758487.2
Gallus gallus (chicken)	FUK	XP_001233364.3
Xenopus tropicalis (tropical clawed frog)	fuk	XP_004914102.1
Oryza sativa (rice)	Os03g0115100	NP_001048749.1
Homo sapiens (human)	FUK	NP_659496.2
Bos taurus (cattle)	FUK	NP_001093852.1
Danio rerio (zebrafish)	LOC100150934	XP_003201444.2
Caenorhabditis elegans (roundworm)	C26D10.4	NP_495756.2
Macaca mulatta (Rhesus monkey)	FUK	XP_001107211.1
Rattus norvegicus (Norway rat)	Fuk	NP_001100899.1
Arabidopsis thaliana (thale cress)	FKGP	NP_563620.1

Gene Ontology
Bibliography

Related articles in PubMed

Pathogenic Variants in Fucokinase Cause a Congenital Disorder of Glycosylation.
Ng BG, Rosenfeld JA, Emrick L, Jain M, Burrage LC, Lee B, , Craigen WJ, Bearden DR, Graham BH, Freeze HH
American journal of human genetics103(6)1030-1037(2018 12)

Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose.
Niittymäki J, Mattila P, Roos C, Huopaniemi L, Sjöblom S, Renkonen R
European journal of biochemistry271(1)78-86(2004 Jan)

Dopamine stimulated L-fucose incorporation into brain proteins is related to an increase in fucokinase activity.
Jork R, Schmitt M, Lössner B, Matthies H
Biomedica biochimica acta43(3)261-70(1984)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fuk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fuk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05341	NM_001107429.1 Latest version!	Rattus norvegicus fucose kinase (Fcsk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa38939	XM_006255624.3 Latest version!	Rattus norvegicus fucokinase (Fuk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fuk ( NM_001107429.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100899.1 Rattus norvegicus fucose kinase (Fcsk), mRNA.

Price & Availability↑

CloneID	ORa05341
Clone ID Related Accession (Same CDS sequence)	NM_001107429.1
Accession Version	NM_001107429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Rattus norvegicus(Norway rat)
Product	L-fucose kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473972.1. On or before Oct 4, 2007 this sequence version replaced XM_001077423.1, XM_226508.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACCAGC CAAAGGGGGT CAATTGGACG GTCATTATCC TGACATGCCA GTACAAGGAC 
AGTGTCCAGG TCTTTCAGAG AGAGCTGGAG GTAAGGCAGA AGCGGGAGCA GATCCCTGCC 
GGGACGATGT TACTGGCTGT GGAGGACCCC CAGACCCGAG TAGGCAGTGG AGGAGCTACT 
CTCAATGCAC TGCTGGTGGC TGCTGAGCAC CTGAGTGCCC GAGCTGGCTT CACCGTGGTC 
ACGTCAGATG TCCTGCACTC GGCTTGGATT CTCATCTTGC ACATGGGCCG AGACTTCCCC 
TTTGATGACT GTGGCAGGGC CTTCACTTGC CTCCCTGTGG AGAATCCACA GGCCCCTGTG 
GAGGCCTTGG TATGCAACCT GGACTGCCTG TTGGATATCA TGACCCACCG GCTGGGTCCA 
GGATCCCCAC CAGGTGTGTG GGTCTGCAGC ACCGACATGC TTCTGTCTGT TCCTCCAAAC 
CCTGGGATCA GTTGGGATGG CTTCCGGGGA ACCAGAGTGA TCGCCTTTCC TGGGAGCCTG 
GCCTACGCTC TAAACCACGG GGTCTACCTC ACTGACTCGC AGGGCGTGGT TTTGGACATT 
TACTACCAGG GCACTAAGGC AGAGATACAA CGGTGTGTCA GGCCTGATGG ACTGGTACCA 
CTGGTCTCTG GGGTTGTCTT CTTCTCTGTG GAGACTGCTG AGCACCTCCT AGCCACCCAC 
GTGAGCCCAC CGCTGGACGC CTGCACCTAT ATGGGCTTGG ACTCTGGAGC CCAGCCTGTG 
CAGCTGTCTC TGTTTTTCGA CATCCTGCTC TGCATGGCTC GGAATATGAG CAGGGAGAAC 
TTCGTGGCTG GGCGGCCCCC GGAGATGGGG CAAGGTGACC CGGATGTAGC ACGTTACCTG 
AAGGGAGCCC GGGCCCAGCT GTGGAGGGAG CTTCGAGATC AGCCCCTCAC TATGGTGTAT 
GTCCCTGATG GCGGTTACAG TTACATGACA ACTGATGCCA CGGAGTTCCT GCACAGACTC 
ACGATGCCTG GAGTAGCTGT GGCCCAGATT GTTCACTCTC AGGTGGAGGA GCCACAGCTG 
CTAGAGGCTA CGTGCTCCGT GGTCAGCTGC CTGCTGGAGG GTCCCGTGCA CCTGGGGCCT 
CGAAGTGTCC TGCAGCACTG TCACCTGAGG GGCCCCATTC ATATTGGCGC TGGCTGCTTT 
GTGAGTGGCC TGGATACCGC CCACTCCGAG GCACTGCATG GCCTGGAGCT TCATGACCTC 
ATCCTTCAGG GACACCACAT ACGGCTGCAT GGCTCCCAGA GTCGTGTATT CACTCTTGCT 
GGCCGTCTGG ACAGCTGGGA AAGACAGGGG GCAGGCATGT ATCTCAACAT GTCCTGGAAT 
GAGTTCTTCA AGAAGACAGG CATTCGAGAC TGGGACCTGT GGGACCCAGA TACACCCCTC 
TCAGATCGAT GCCTTCTCAG TGCCCGCCTT TTCCCTGTGC TCCACCCCAC GAGGGCTCTG 
GGGCCCCAGG ATGTGCTGTG GATGCTGCAT CCTCATAAGG ACAGAGGCGA GGCCCTGCGT 
GCCTGGAGAG CCTCCTGGCG TCTGTCCTGG GAGCAGCTGC AACCTCGCCT GGACCGGGCT 
GCCACACTGG ACTTCCGTCG GGATCTGTTC TTCCGCCAGG CCTTGCAGAA GGCGAGGCAT 
GTGTTAGAGG CCCGGCAGGA CCTCTGCCTA CATCCACTGA TCCGGGCTGC TGTCGGTGAA 
GGTTGCTCTG GGCCCCTGCT GGCCACACTT GACAAGGTTG CAGCAGGGGC AGAAGATCCT 
GGTGTGGCAG CCCGGGCTCT GGCTTGTGTG GCAGATGTAC TCGGCTGCAT GGCAGAGGGC 
CAAGGAGGCT TGCGCAGTGG GCCAGCTGCC AACCCTGAGT GGATTCAGCC TTTCTCATAC 
TTGGAACGTG GAGACCTCAT GAGGGGTGTG GAGGCACTTG CCCAGGAAAG AGAGAAGTGG 
CTGACCAGGC CTGCCTTGTT GGTTCGAGCT GCCCGCCATT ATGAGGGGGC TGAGCAGATC 
CTGATCCGAC AGGCTGTGAT GACAGCCCGG CACTTCGTCT CCACCCAGCC AGTGGAATTG 
CCAGCACCTG GGCAGTGGGT GGTGACTGAG TGCCCAGCCC GTGTGGATTT CTCTGGGGGC 
TGGAGTGACA CACCACCCAT TGCCTATGAG CTTGGTGGAG CAGTATTGGG CCTGGCTGTT 
CGGGTGGATG GCCGCCGGCC CATCGGGGCC AAGGCACGCC GCATCCTAGA GCCTGAGCTC 
TGGCTGGCAG TGGGACCTCG ACAGGATGAG ATGACCGTGA AGATAGTGTG CCGGAGCCTT 
GATGACCTGC AGGATTACTG CCAGCCTCAT GCCCCAGGTG CCTTGCTGAA GGCAGCCTTT 
ATCTGTGCGG ATATTGTGCA TGTCAACTCA GAGGTCCCTC TGCATGAACA GTTGCTACGC 
TCGTTTAATG GTGGCTTTGA GCTGCACACA TGGTCAGAGC TGCCACACGG CTCTGGTCTT 
GGCACTAGCA GCATCTTGGC AGGGGCTGCC CTGGCTGCTT TGCAGCGGGC TGCAGGCCGG 
ACAGTGGGCA CAGAGGCTCT CATCCATGCA GTGTTGCACC TGGAGCAGGT GCTCACCACA 
GGAGGTGGCT GGCAGGACCA AGTGAGTGGC CTAATGCCTG GCATCAAGGT GGGGCGCTCT 
CGGGCACAGC TGCCCCTAAA GGTGGAGGTG GAGGAAATCA CTGTGCCTGA GAACTTTGTC 
CAGAGGAAGC TTATGGCCCC AGGCTGTGAG CCGCTGGCTG TGCATCGGAT GATGGATGTC 
CTGGCCCCTT ATGCCTTCGG CCAAAGTCTG GCAGGGGCAG GCGGTGGGGG CTTTCTCTAT 
CTGTTGACCA AGGAACCCCG GCAGAAAGAG GTCCTAGAAG CTGTGCTGGC CAAGGTGGAG 
GGCCTCGGCA ACTACAGCGT CCACCTGGTG CAAGTGGACA CTCAGGGCCT GAGCCTGCAG 
CTGCTAGGAC ATGACGCCCA TCTTTGCGGG GCTGGGCCCT CTGAAGTGGG CAACACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100899.1
CDS	62..3121
Translation	MDQPKGVNWTVIILTCQYKDSVQVFQRELEVRQKREQIPAGTMLLAVEDPQTRVGSGGATLNALLVAAEHLSARAGFTVVTSDVLHSAWILILHMGRDFPFDDCGRAFTCLPVENPQAPVEALVCNLDCLLDIMTHRLGPGSPPGVWVCSTDMLLSVPPNPGISWDGFRGTRVIAFPGSLAYALNHGVYLTDSQGVVLDIYYQGTKAEIQRCVRPDGLVPLVSGVVFFSVETAEHLLATHVSPPLDACTYMGLDSGAQPVQLSLFFDILLCMARNMSRENFVAGRPPEMGQGDPDVARYLKGARAQLWRELRDQPLTMVYVPDGGYSYMTTDATEFLHRLTMPGVAVAQIVHSQVEEPQLLEATCSVVSCLLEGPVHLGPRSVLQHCHLRGPIHIGAGCFVSGLDTAHSEALHGLELHDLILQGHHIRLHGSQSRVFTLAGRLDSWERQGAGMYLNMSWNEFFKKTGIRDWDLWDPDTPLSDRCLLSARLFPVLHPTRALGPQDVLWMLHPHKDRGEALRAWRASWRLSWEQLQPRLDRAATLDFRRDLFFRQALQKARHVLEARQDLCLHPLIRAAVGEGCSGPLLATLDKVAAGAEDPGVAARALACVADVLGCMAEGQGGLRSGPAANPEWIQPFSYLERGDLMRGVEALAQEREKWLTRPALLVRAARHYEGAEQILIRQAVMTARHFVSTQPVELPAPGQWVVTECPARVDFSGGWSDTPPIAYELGGAVLGLAVRVDGRRPIGAKARRILEPELWLAVGPRQDEMTVKIVCRSLDDLQDYCQPHAPGALLKAAFICADIVHVNSEVPLHEQLLRSFNGGFELHTWSELPHGSGLGTSSILAGAALAALQRAAGRTVGTEALIHAVLHLEQVLTTGGGWQDQVSGLMPGIKVGRSRAQLPLKVEVEEITVPENFVQRKLMAPGCEPLAVHRMMDVLAPYAFGQSLAGAGGGGFLYLLTKEPRQKEVLEAVLAKVEGLGNYSVHLVQVDTQGLSLQLLGHDAHLCGAGPSEVGNT

Target ORF information:

RefSeq Version	NM_001107429.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fucose kinase (Fcsk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107429.1

ORF Insert Sequence:

ATGGACCAGC CAAAGGGGGT CAATTGGACG GTCATTATCC TGACATGCCA GTACAAGGAC 
AGTGTCCAGG TCTTTCAGAG AGAGCTGGAG GTAAGGCAGA AGCGGGAGCA GATCCCTGCC 
GGGACGATGT TACTGGCTGT GGAGGACCCC CAGACCCGAG TAGGCAGTGG AGGAGCTACT 
CTCAATGCAC TGCTGGTGGC TGCTGAGCAC CTGAGTGCCC GAGCTGGCTT CACCGTGGTC 
ACGTCAGATG TCCTGCACTC GGCTTGGATT CTCATCTTGC ACATGGGCCG AGACTTCCCC 
TTTGATGACT GTGGCAGGGC CTTCACTTGC CTCCCTGTGG AGAATCCACA GGCCCCTGTG 
GAGGCCTTGG TATGCAACCT GGACTGCCTG TTGGATATCA TGACCCACCG GCTGGGTCCA 
GGATCCCCAC CAGGTGTGTG GGTCTGCAGC ACCGACATGC TTCTGTCTGT TCCTCCAAAC 
CCTGGGATCA GTTGGGATGG CTTCCGGGGA ACCAGAGTGA TCGCCTTTCC TGGGAGCCTG 
GCCTACGCTC TAAACCACGG GGTCTACCTC ACTGACTCGC AGGGCGTGGT TTTGGACATT 
TACTACCAGG GCACTAAGGC AGAGATACAA CGGTGTGTCA GGCCTGATGG ACTGGTACCA 
CTGGTCTCTG GGGTTGTCTT CTTCTCTGTG GAGACTGCTG AGCACCTCCT AGCCACCCAC 
GTGAGCCCAC CGCTGGACGC CTGCACCTAT ATGGGCTTGG ACTCTGGAGC CCAGCCTGTG 
CAGCTGTCTC TGTTTTTCGA CATCCTGCTC TGCATGGCTC GGAATATGAG CAGGGAGAAC 
TTCGTGGCTG GGCGGCCCCC GGAGATGGGG CAAGGTGACC CGGATGTAGC ACGTTACCTG 
AAGGGAGCCC GGGCCCAGCT GTGGAGGGAG CTTCGAGATC AGCCCCTCAC TATGGTGTAT 
GTCCCTGATG GCGGTTACAG TTACATGACA ACTGATGCCA CGGAGTTCCT GCACAGACTC 
ACGATGCCTG GAGTAGCTGT GGCCCAGATT GTTCACTCTC AGGTGGAGGA GCCACAGCTG 
CTAGAGGCTA CGTGCTCCGT GGTCAGCTGC CTGCTGGAGG GTCCCGTGCA CCTGGGGCCT 
CGAAGTGTCC TGCAGCACTG TCACCTGAGG GGCCCCATTC ATATTGGCGC TGGCTGCTTT 
GTGAGTGGCC TGGATACCGC CCACTCCGAG GCACTGCATG GCCTGGAGCT TCATGACCTC 
ATCCTTCAGG GACACCACAT ACGGCTGCAT GGCTCCCAGA GTCGTGTATT CACTCTTGCT 
GGCCGTCTGG ACAGCTGGGA AAGACAGGGG GCAGGCATGT ATCTCAACAT GTCCTGGAAT 
GAGTTCTTCA AGAAGACAGG CATTCGAGAC TGGGACCTGT GGGACCCAGA TACACCCCTC 
TCAGATCGAT GCCTTCTCAG TGCCCGCCTT TTCCCTGTGC TCCACCCCAC GAGGGCTCTG 
GGGCCCCAGG ATGTGCTGTG GATGCTGCAT CCTCATAAGG ACAGAGGCGA GGCCCTGCGT 
GCCTGGAGAG CCTCCTGGCG TCTGTCCTGG GAGCAGCTGC AACCTCGCCT GGACCGGGCT 
GCCACACTGG ACTTCCGTCG GGATCTGTTC TTCCGCCAGG CCTTGCAGAA GGCGAGGCAT 
GTGTTAGAGG CCCGGCAGGA CCTCTGCCTA CATCCACTGA TCCGGGCTGC TGTCGGTGAA 
GGTTGCTCTG GGCCCCTGCT GGCCACACTT GACAAGGTTG CAGCAGGGGC AGAAGATCCT 
GGTGTGGCAG CCCGGGCTCT GGCTTGTGTG GCAGATGTAC TCGGCTGCAT GGCAGAGGGC 
CAAGGAGGCT TGCGCAGTGG GCCAGCTGCC AACCCTGAGT GGATTCAGCC TTTCTCATAC 
TTGGAACGTG GAGACCTCAT GAGGGGTGTG GAGGCACTTG CCCAGGAAAG AGAGAAGTGG 
CTGACCAGGC CTGCCTTGTT GGTTCGAGCT GCCCGCCATT ATGAGGGGGC TGAGCAGATC 
CTGATCCGAC AGGCTGTGAT GACAGCCCGG CACTTCGTCT CCACCCAGCC AGTGGAATTG 
CCAGCACCTG GGCAGTGGGT GGTGACTGAG TGCCCAGCCC GTGTGGATTT CTCTGGGGGC 
TGGAGTGACA CACCACCCAT TGCCTATGAG CTTGGTGGAG CAGTATTGGG CCTGGCTGTT 
CGGGTGGATG GCCGCCGGCC CATCGGGGCC AAGGCACGCC GCATCCTAGA GCCTGAGCTC 
TGGCTGGCAG TGGGACCTCG ACAGGATGAG ATGACCGTGA AGATAGTGTG CCGGAGCCTT 
GATGACCTGC AGGATTACTG CCAGCCTCAT GCCCCAGGTG CCTTGCTGAA GGCAGCCTTT 
ATCTGTGCGG ATATTGTGCA TGTCAACTCA GAGGTCCCTC TGCATGAACA GTTGCTACGC 
TCGTTTAATG GTGGCTTTGA GCTGCACACA TGGTCAGAGC TGCCACACGG CTCTGGTCTT 
GGCACTAGCA GCATCTTGGC AGGGGCTGCC CTGGCTGCTT TGCAGCGGGC TGCAGGCCGG 
ACAGTGGGCA CAGAGGCTCT CATCCATGCA GTGTTGCACC TGGAGCAGGT GCTCACCACA 
GGAGGTGGCT GGCAGGACCA AGTGAGTGGC CTAATGCCTG GCATCAAGGT GGGGCGCTCT 
CGGGCACAGC TGCCCCTAAA GGTGGAGGTG GAGGAAATCA CTGTGCCTGA GAACTTTGTC 
CAGAGGAAGC TTATGGCCCC AGGCTGTGAG CCGCTGGCTG TGCATCGGAT GATGGATGTC 
CTGGCCCCTT ATGCCTTCGG CCAAAGTCTG GCAGGGGCAG GCGGTGGGGG CTTTCTCTAT 
CTGTTGACCA AGGAACCCCG GCAGAAAGAG GTCCTAGAAG CTGTGCTGGC CAAGGTGGAG 
GGCCTCGGCA ACTACAGCGT CCACCTGGTG CAAGTGGACA CTCAGGGCCT GAGCCTGCAG 
CTGCTAGGAC ATGACGCCCA TCTTTGCGGG GCTGGGCCCT CTGAAGTGGG CAACACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fuk ( XM_006255624.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255686.2 Rattus norvegicus fucokinase (Fuk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38939
Clone ID Related Accession (Same CDS sequence)	XM_006255624.3
Accession Version	XM_006255624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	L-fucose kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255624.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGC CAAAGGGGGT CAATTGGACG GTCATTATCC TGACATGCCA GTACAAGGAC 
AGTGTCCAGG TCTTTCAGAG AGAGCTGGAG GTAAGGCAGA AGCGGGAGCA GATCCCTGCC 
GGGACGATGT TACTGGCTGT GGAGGACCCC CAGACCCGAG TAGGCAGTGG AGGAGCTACT 
CTCAATGCAC TGCTGGTGGC TGCTGAGCAC CTGAGTGCCC GAGCTGGCTT CACCGTGGTC 
ACGTCAGATG TCCTGCACTC GGCTTGGATT CTCATCTTGC ACATGGGCCG AGACTTCCCC 
TTTGATGACT GTGGCAGGGC CTTCACTTGC CTCCCTGTGG AGAATCCACA GGCCCCTGTG 
GAGGCCTTGG TATGCAACCT GGACTGCCTG TTGGATATCA TGACCCACCG GCTGGGTCCA 
GGATCCCCAC CAGGTGTGTG GGTCTGCAGC ACCGACATGC TTCTGTCTGT TCCTCCAAAC 
CCTGGGATCA GTTGGGATGG CTTCCGGGGA ACCAGAGTGA TCGCCTTTCC TGGGAGCCTG 
GCCTACGCTC TAAACCACGG GGTCTACCTC ACTGACTCGC AGGGCGTGGT TTTGGACATT 
TACTACCAGG GCACTAAGGC AGAGATACAA CGGTGTGTCA GGCCTGATGG ACTGGTACCA 
CTGGTCTCTG GGGTTGTCTT CTTCTCTGTG GAGACTGCTG AGCACCTCCT AGCCACCCAC 
GTGAGCCCAC CGCTGGACGC CTGCACCTAT ATGGGCTTGG ACTCTGGAGC CCAGCCTGTG 
CAGCTGTCTC TGTTTTTCGA CATCCTGCTC TGCATGGCTC GGAATATGAG CAGGGAGAAC 
TTCGTGGCTG GGCGGCCCCC GGAGATGGGG CAAGGTGACC CGGATGTAGC ACGTTACCTG 
AAGGGAGCCC GGGCCCAGCT GTGGAGGGAG CTTCGAGATC AGCCCCTCAC TATGGTGTAT 
GTCCCTGATG GCGGTTACAG TTACATGACA ACTGATGCCA CGGAGTTCCT GCACAGACTC 
ACGATGCCTG GAGTAGCTGT GGCCCAGATT GTTCACTCTC AGGTGGAGGA GCCACAGCTG 
CTAGAGGCTA CGTGCTCCGT GGTCAGCTGC CTGCTGGAGG GTCCCGTGCA CCTGGGGCCT 
CGAAGTGTCC TGCAGCACTG TCACCTGAGG GGCCCCATTC ATATTGGCGC TGGCTGCTTT 
GTGAGTGGCC TGGATACCGC CCACTCCGAG GCACTGCATG GCCTGGAGCT TCATGACCTC 
ATCCTTCAGG GACACCACAT ACGGCTGCAT GGCTCCCAGA GTCGTGTATT CACTCTTGCT 
GGCCGTCTGG ACAGCTGGGA AAGACAGGGG GCAGGCATGT ATCTCAACAT GTCCTGGAAT 
GAGTTCTTCA AGAAGACAGG CATTCGAGAC TGGGACCTGT GGGACCCAGA TACACCCCTC 
TCAGATCGAT GCCTTCTCAG TGCCCGCCTT TTCCCTGTGC TCCACCCCAC GAGGGCTCTG 
GGGCCCCAGG ATGTGCTGTG GATGCTGCAT CCTCATAAGG ACAGAGGCGA GGCCCTGCGT 
GCCTGGAGAG CCTCCTGGCG TCTGTCCTGG GAGCAGCTGC AACCTCGCCT GGACCGGGCT 
GCCACACTGG ACTTCCGTCG GGATCTGTTC TTCCGCCAGG CCTTGCAGAA GGCGAGGCAT 
GTGTTAGAGG CCCGGCAGGA CCTCTGCCTA CATCCACTGA TCCGGGCTGC TGTCGGTGAA 
GGTTGCTCTG GGCCCCTGCT GGCCACACTT GACAAGGTTG CAGCAGGGGC AGAAGATCCT 
GGTGTGGCAG CCCGGGCTCT GGCTTGTGTG GCAGATGTAC TCGGCTGCAT GGCAGAGGGC 
CAAGGAGGCT TGCGCAGTGG GCCAGCTGCC AACCCTGAGT GGATTCAGCC TTTCTCATAC 
TTGGAACGTG GAGACCTCAT GAGGGGTGTG GAGGCACTTG CCCAGGAAAG AGAGAAGTGG 
CTGACCAGGC CTGCCTTGTT GGTTCGAGCT GCCCGCCATT ATGAGGGGGC TGAGCAGATC 
CTGATCCGAC AGGCTGTGAT GACAGCCCGG CACTTCGTCT CCACCCAGCC AGTGGAATTG 
CCAGCACCTG GGCAGTGGGT GGTGACTGAG TGCCCAGCCC GTGTGGATTT CTCTGGGGGC 
TGGAGTGACA CACCACCCAT TGCCTATGAG CTTGGTGGAG CAGTATTGGG CCTGGCTGTT 
CGGGTGGATG GCCGCCGGCC CATCGGGGCC AAGGCACGCC GCATCCTAGA GCCTGAGCTC 
TGGCTGGCAG TGGGACCTCG ACAGGATGAG ATGACCGTGA AGATAGTGTG CCGGAGCCTT 
GATGACCTGC AGGATTACTG CCAGCCTCAT GCCCCAGGTG CCTTGCTGAA GGCAGCCTTT 
ATCTGTGCGG ATATTGTGCA TGTCAACTCA GAGGTCCCTC TGCATGAACA GTTGCTACGC 
TCGTTTAATG GTGGCTTTGA GCTGCACACA TGGTCAGAGC TGCCACACGG CTCTGGTCTT 
GGCACTAGCA GCATCTTGGC AGGGGCTGCC CTGGCTGCTT TGCAGCGGGC TGCAGGCCGG 
ACAGTGGGCA CAGAGGCTCT CATCCATGCA GTGTTGCACC TGGAGCAGGT GCTCACCACA 
GGAGGTGGCT GGCAGGACCA AGTGAGTGGC CTAATGCCTG GCATCAAGGT GGGGCGCTCT 
CGGGCACAGC TGCCCCTAAA GGTGGAGGTG GAGGAAATCA CTGTGCCTGA GAACTTTGTC 
CAGAGGATCA ATGACCATCT GCTCCTGGTT TATACTGGCA AGACCCGGCT GGCACGGAAT 
CTGCTGCAGG ACGTGCTAAG GAACTGGTAT GCTCGGTTGC CTGTTGTGGT ACAGAATGCC 
CGCAGACTGG TTCGACAGAC TGAGAAGTGT GCTGAAGCTT TCCGCCAAGG AAACTTGGCT 
CTGCTGGGAC AGTACCTGAC CTCATACTGG GAGCAGAAGA AGCTTATGGC CCCAGGCTGT 
GAGCCGCTGG CTGTGCATCG GATGATGGAT GTCCTGGCCC CTTATGCCTT CGGCCAAAGT 
CTGGCAGGGG CAGGCGGTGG GGGCTTTCTC TATCTGTTGA CCAAGGAACC CCGGCAGAAA 
GAGGTCCTAG AAGCTGTGCT GGCCAAGGTG GAGGGCCTCG GCAACTACAG CGTCCACCTG 
GTGCAAGTGG ACACTCAGGG CCTGAGCCTG CAGCTGCTAG GACATGACGC CCATCTTTGC 
GGGGCTGGGC CCTCTGAAGT GGGCAACACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255686.2
CDS	87..3359
Translation	MDQPKGVNWTVIILTCQYKDSVQVFQRELEVRQKREQIPAGTMLLAVEDPQTRVGSGGATLNALLVAAEHLSARAGFTVVTSDVLHSAWILILHMGRDFPFDDCGRAFTCLPVENPQAPVEALVCNLDCLLDIMTHRLGPGSPPGVWVCSTDMLLSVPPNPGISWDGFRGTRVIAFPGSLAYALNHGVYLTDSQGVVLDIYYQGTKAEIQRCVRPDGLVPLVSGVVFFSVETAEHLLATHVSPPLDACTYMGLDSGAQPVQLSLFFDILLCMARNMSRENFVAGRPPEMGQGDPDVARYLKGARAQLWRELRDQPLTMVYVPDGGYSYMTTDATEFLHRLTMPGVAVAQIVHSQVEEPQLLEATCSVVSCLLEGPVHLGPRSVLQHCHLRGPIHIGAGCFVSGLDTAHSEALHGLELHDLILQGHHIRLHGSQSRVFTLAGRLDSWERQGAGMYLNMSWNEFFKKTGIRDWDLWDPDTPLSDRCLLSARLFPVLHPTRALGPQDVLWMLHPHKDRGEALRAWRASWRLSWEQLQPRLDRAATLDFRRDLFFRQALQKARHVLEARQDLCLHPLIRAAVGEGCSGPLLATLDKVAAGAEDPGVAARALACVADVLGCMAEGQGGLRSGPAANPEWIQPFSYLERGDLMRGVEALAQEREKWLTRPALLVRAARHYEGAEQILIRQAVMTARHFVSTQPVELPAPGQWVVTECPARVDFSGGWSDTPPIAYELGGAVLGLAVRVDGRRPIGAKARRILEPELWLAVGPRQDEMTVKIVCRSLDDLQDYCQPHAPGALLKAAFICADIVHVNSEVPLHEQLLRSFNGGFELHTWSELPHGSGLGTSSILAGAALAALQRAAGRTVGTEALIHAVLHLEQVLTTGGGWQDQVSGLMPGIKVGRSRAQLPLKVEVEEITVPENFVQRINDHLLLVYTGKTRLARNLLQDVLRNWYARLPVVVQNARRLVRQTEKCAEAFRQGNLALLGQYLTSYWEQKKLMAPGCEPLAVHRMMDVLAPYAFGQSLAGAGGGGFLYLLTKEPRQKEVLEAVLAKVEGLGNYSVHLVQVDTQGLSLQLLGHDAHLCGAGPSEVGNT

Target ORF information:

RefSeq Version	XM_006255624.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fucokinase (Fuk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255624.3

ORF Insert Sequence:

ATGGACCAGC CAAAGGGGGT CAATTGGACG GTCATTATCC TGACATGCCA GTACAAGGAC 
AGTGTCCAGG TCTTTCAGAG AGAGCTGGAG GTAAGGCAGA AGCGGGAGCA GATCCCTGCC 
GGGACGATGT TACTGGCTGT GGAGGACCCC CAGACCCGAG TAGGCAGTGG AGGAGCTACT 
CTCAATGCAC TGCTGGTGGC TGCTGAGCAC CTGAGTGCCC GAGCTGGCTT CACCGTGGTC 
ACGTCAGATG TCCTGCACTC GGCTTGGATT CTCATCTTGC ACATGGGCCG AGACTTCCCC 
TTTGATGACT GTGGCAGGGC CTTCACTTGC CTCCCTGTGG AGAATCCACA GGCCCCTGTG 
GAGGCCTTGG TATGCAACCT GGACTGCCTG TTGGATATCA TGACCCACCG GCTGGGTCCA 
GGATCCCCAC CAGGTGTGTG GGTCTGCAGC ACCGACATGC TTCTGTCTGT TCCTCCAAAC 
CCTGGGATCA GTTGGGATGG CTTCCGGGGA ACCAGAGTGA TCGCCTTTCC TGGGAGCCTG 
GCCTACGCTC TAAACCACGG GGTCTACCTC ACTGACTCGC AGGGCGTGGT TTTGGACATT 
TACTACCAGG GCACTAAGGC AGAGATACAA CGGTGTGTCA GGCCTGATGG ACTGGTACCA 
CTGGTCTCTG GGGTTGTCTT CTTCTCTGTG GAGACTGCTG AGCACCTCCT AGCCACCCAC 
GTGAGCCCAC CGCTGGACGC CTGCACCTAT ATGGGCTTGG ACTCTGGAGC CCAGCCTGTG 
CAGCTGTCTC TGTTTTTCGA CATCCTGCTC TGCATGGCTC GGAATATGAG CAGGGAGAAC 
TTCGTGGCTG GGCGGCCCCC GGAGATGGGG CAAGGTGACC CGGATGTAGC ACGTTACCTG 
AAGGGAGCCC GGGCCCAGCT GTGGAGGGAG CTTCGAGATC AGCCCCTCAC TATGGTGTAT 
GTCCCTGATG GCGGTTACAG TTACATGACA ACTGATGCCA CGGAGTTCCT GCACAGACTC 
ACGATGCCTG GAGTAGCTGT GGCCCAGATT GTTCACTCTC AGGTGGAGGA GCCACAGCTG 
CTAGAGGCTA CGTGCTCCGT GGTCAGCTGC CTGCTGGAGG GTCCCGTGCA CCTGGGGCCT 
CGAAGTGTCC TGCAGCACTG TCACCTGAGG GGCCCCATTC ATATTGGCGC TGGCTGCTTT 
GTGAGTGGCC TGGATACCGC CCACTCCGAG GCACTGCATG GCCTGGAGCT TCATGACCTC 
ATCCTTCAGG GACACCACAT ACGGCTGCAT GGCTCCCAGA GTCGTGTATT CACTCTTGCT 
GGCCGTCTGG ACAGCTGGGA AAGACAGGGG GCAGGCATGT ATCTCAACAT GTCCTGGAAT 
GAGTTCTTCA AGAAGACAGG CATTCGAGAC TGGGACCTGT GGGACCCAGA TACACCCCTC 
TCAGATCGAT GCCTTCTCAG TGCCCGCCTT TTCCCTGTGC TCCACCCCAC GAGGGCTCTG 
GGGCCCCAGG ATGTGCTGTG GATGCTGCAT CCTCATAAGG ACAGAGGCGA GGCCCTGCGT 
GCCTGGAGAG CCTCCTGGCG TCTGTCCTGG GAGCAGCTGC AACCTCGCCT GGACCGGGCT 
GCCACACTGG ACTTCCGTCG GGATCTGTTC TTCCGCCAGG CCTTGCAGAA GGCGAGGCAT 
GTGTTAGAGG CCCGGCAGGA CCTCTGCCTA CATCCACTGA TCCGGGCTGC TGTCGGTGAA 
GGTTGCTCTG GGCCCCTGCT GGCCACACTT GACAAGGTTG CAGCAGGGGC AGAAGATCCT 
GGTGTGGCAG CCCGGGCTCT GGCTTGTGTG GCAGATGTAC TCGGCTGCAT GGCAGAGGGC 
CAAGGAGGCT TGCGCAGTGG GCCAGCTGCC AACCCTGAGT GGATTCAGCC TTTCTCATAC 
TTGGAACGTG GAGACCTCAT GAGGGGTGTG GAGGCACTTG CCCAGGAAAG AGAGAAGTGG 
CTGACCAGGC CTGCCTTGTT GGTTCGAGCT GCCCGCCATT ATGAGGGGGC TGAGCAGATC 
CTGATCCGAC AGGCTGTGAT GACAGCCCGG CACTTCGTCT CCACCCAGCC AGTGGAATTG 
CCAGCACCTG GGCAGTGGGT GGTGACTGAG TGCCCAGCCC GTGTGGATTT CTCTGGGGGC 
TGGAGTGACA CACCACCCAT TGCCTATGAG CTTGGTGGAG CAGTATTGGG CCTGGCTGTT 
CGGGTGGATG GCCGCCGGCC CATCGGGGCC AAGGCACGCC GCATCCTAGA GCCTGAGCTC 
TGGCTGGCAG TGGGACCTCG ACAGGATGAG ATGACCGTGA AGATAGTGTG CCGGAGCCTT 
GATGACCTGC AGGATTACTG CCAGCCTCAT GCCCCAGGTG CCTTGCTGAA GGCAGCCTTT 
ATCTGTGCGG ATATTGTGCA TGTCAACTCA GAGGTCCCTC TGCATGAACA GTTGCTACGC 
TCGTTTAATG GTGGCTTTGA GCTGCACACA TGGTCAGAGC TGCCACACGG CTCTGGTCTT 
GGCACTAGCA GCATCTTGGC AGGGGCTGCC CTGGCTGCTT TGCAGCGGGC TGCAGGCCGG 
ACAGTGGGCA CAGAGGCTCT CATCCATGCA GTGTTGCACC TGGAGCAGGT GCTCACCACA 
GGAGGTGGCT GGCAGGACCA AGTGAGTGGC CTAATGCCTG GCATCAAGGT GGGGCGCTCT 
CGGGCACAGC TGCCCCTAAA GGTGGAGGTG GAGGAAATCA CTGTGCCTGA GAACTTTGTC 
CAGAGGATCA ATGACCATCT GCTCCTGGTT TATACTGGCA AGACCCGGCT GGCACGGAAT 
CTGCTGCAGG ACGTGCTAAG GAACTGGTAT GCTCGGTTGC CTGTTGTGGT ACAGAATGCC 
CGCAGACTGG TTCGACAGAC TGAGAAGTGT GCTGAAGCTT TCCGCCAAGG AAACTTGGCT 
CTGCTGGGAC AGTACCTGAC CTCATACTGG GAGCAGAAGA AGCTTATGGC CCCAGGCTGT 
GAGCCGCTGG CTGTGCATCG GATGATGGAT GTCCTGGCCC CTTATGCCTT CGGCCAAAGT 
CTGGCAGGGG CAGGCGGTGG GGGCTTTCTC TATCTGTTGA CCAAGGAACC CCGGCAGAAA 
GAGGTCCTAG AAGCTGTGCT GGCCAAGGTG GAGGGCCTCG GCAACTACAG CGTCCACCTG 
GTGCAAGTGG ACACTCAGGG CCTGAGCCTG CAGCTGCTAG GACATGACGC CCATCTTTGC 
GGGGCTGGGC CCTCTGAAGT GGGCAACACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pathogenic Variants in Fucokinase Cause a Congenital Disorder of Glycosylation.
American journal of human genetics103(6)1030-1037(2018 12)
Ng BG,Rosenfeld JA,Emrick L,Jain M,Burrage LC,Lee B,,Craigen WJ,Bearden DR,Graham BH,Freeze HH

Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose.
European journal of biochemistry271(1)78-86(2004 Jan)
Niittymäki J,Mattila P,Roos C,Huopaniemi L,Sjöblom S,Renkonen R

Dopamine stimulated L-fucose incorporation into brain proteins is related to an increase in fucokinase activity.
Biomedica biochimica acta43(3)261-70(1984)
Jork R,Schmitt M,Lössner B,Matthies H

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Fuk cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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