Cacna2d3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna2d3
Entrez Gene ID	306243
Full Name	calcium voltage-gated channel auxiliary subunit alpha2delta 3
General protein information	Preferred Names calcium voltage-gated channel auxiliary subunit alpha2delta 3 Names voltage-dependent calcium channel subunit alpha-2/delta-3 calcium channel, voltage-dependent, alpha 2/delta 3 subunit calcium channel, voltage-dependent, alpha2/delta subunit 3 voltage-gated calcium channel subunit alpha-2/delta-3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p16
Summary	auxillary subinit of the voltage dependent calcium channel; may be involved in calcium current in the heart [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_175595.2	NP_783185.1	voltage-dependent calcium channel subunit alpha-2/delta-3 precursor
XM_017600076.1	XP_017455565.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
XM_017600077.1	XP_017455566.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA2D3	NP_060868.2
Pan troglodytes (chimpanzee)	CACNA2D3	XP_516539.3
Canis lupus familiaris (dog)	CACNA2D3	XP_005633241.1
Mus musculus (house mouse)	Cacna2d3	NP_033915.1
Rattus norvegicus (Norway rat)	Cacna2d3	NP_783185.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005490	XP_315491.4
Bos taurus (cattle)	CACNA2D3	NP_001193680.1
Drosophila melanogaster (fruit fly)	stj	NP_001246303.1
Danio rerio (zebrafish)	cacna2d3	XP_005172562.1
Gallus gallus (chicken)	CACNA2D3	XP_414338.2
Xenopus tropicalis (tropical clawed frog)	cacna2d3	XP_004914404.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina.
Pérez de Sevilla Müller L, Sargoy A, Fernández-Sánchez L, Rodriguez A, Liu J, Cuenca N, Brecha N
The Journal of comparative neurology523(10)1443-60(2015 Jul)

The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for function.
Davies A, Kadurin I, Alvarez-Laviada A, Douglas L, Nieto-Rostro M, Bauer CS, Pratt WS, Dolphin AC
Proceedings of the National Academy of Sciences of the United States of America107(4)1654-9(2010 Jan)

Molecular cloning of calcium channel alpha(2)delta-subunits from rat atria and the differential regulation of their expression by IGF-1.
Chu PJ, Best PM
Journal of molecular and cellular cardiology35(2)207-15(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cacna2d3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna2d3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58796	XM_017600076.1 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa03862	NM_175595.2 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa58797	XM_017600077.1 Latest version!	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna2d3 ( XM_017600076.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455565.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58796
Clone ID Related Accession (Same CDS sequence)	XM_017600076.1
Accession Version	XM_017600076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCGGC CGGGCTCGCT GTGCTGCGCG TCCCGGGGGG CCTCGGCGCT CCTAGCCACC 
GCGCTTCTCT ACGCCGCGCT GGGGGACGTG GTGCGCTCCG AGCAGCAGAT CCCGCTCTCC 
GTAGTGAAGC TCTGGGCCTC CGCTTTTGGT GGGGAGATAA AATCCATTGC TGCCAAGTAC 
TCGGGTTCCC AGCTTCTGCA AAAGAAATAC AAAGAGTATG AGAAAGACGT TGCCATTGAA 
GAAATCGACG GTCTCCAACT GGTGAAAAAG CTGGCCAAGA ACATGGAAGA GATGTTTCAC 
AAGAAGTCCG AGGCAGTGCG GCGTCTCGTG GAGGCTGCAG AGGAAGCACA CCTGAAGCAT 
GAATTTGACG CCGACTTGCA GTATGAATAC TTCAATGCCG TGCTGATCAA CGAGAGAGAC 
AAAGATGGGA ACTTTTTGGA ATTGGGAAAG GAGTTCATCT TGGCCCCCAA TGACCATTTT 
AATAATTTGC CTGTGAACAT CAGTCTGAGT GATGTCCAAG TGCCAACGAA CATGTACAAC 
AAAGATCCTG CCATAGTCAA TGGAGTGTAT TGGTCTGAAT CTCTAAACAA AGTTTTTGTA 
GACAACTTCG ATCGGGACCC GTCTCTTATA TGGCAGTACT TTGGAAGTGC AAAGGGCTTT 
TTCAGACAGT ACCCAGGGAT TAAATGGGAA CCAGACGAGA ATGGAGTCAT TGCCTTTGAC 
TGCAGGAACA GGAAATGGTA CATCCAGGCA GCAACTTCTC CAAAGGATGT GGTCATTTTG 
GTGGACGTCA GCGGGAGCAT GAAAGGACTC CGCCTGACCA TCGCCAAGCA AACAGTCTCC 
TCGATACTGG ACACCCTGGG CGATGATGAC TTCTTCAACA TCATCACGTA TAATGAAGAG 
CTTCACTATG TGGAACCTTG TCTGAATGGA ACACTGGTTC AAGCGGACAG GACCAACAAG 
GAGCACTTCA GGGAGCATTT GGACAAACTT TTTGCCAAAG GGATTGGAAT GCTCGATATT 
GCGCTGAACG AGGCCTTCAA TGTACTGAGC GATTTCAACC ACACCGGACA AGGAAGCATT 
TGCAGCCAGG CCATTATGCT CATAACCGAC GGGGCAGTGG ACACCTACGA CACCATCTTT 
GCAAAGTACA ATTGGCCAGA GCGAAAGGTT CGAATCTTCA CTTACCTCAT TGGACGAGAG 
GCTGCTTTTG CAGACAATCT CAAGTGGATG GCTTGTGCTA ACAAAGGATT TTTCACCCAG 
ATCTCCACCT TGGCTGATGT GCAGGAAAAT GTCATGGAAT ACCTCCATGT ACTCAGTCGA 
CCCAAAGTCA TCGACCAGGA ACATGATGTG GTGTGGACCG AAGCGTACAT CGACAGCACT 
CTTGCTGATG ATCAGGGCCT CGTCTTGATG ACCACAGTGG CCATGCCTGT GTTTAGTAAG 
CAGAACGAAA CTAGGTCAAA GGGCATTCTT CTGGGTGTGG TTGGCACAGA TGTCCCAGTA 
AAAGAGCTTC TGAAGACCAT CCCCAAATAC AAGTTAGGAA TTCATGGTTA TGCCTTTGCC 
ATCACGAATA ATGGATACAT CTTGACACAC CCGGAGCTCA GGCCCCTGTA TGAAGAAGGG 
AAAAAGCGAA GGAAGCCTAA TTACAGTAGT GTGGATCTCT CGGAAGTCGA GTGGGAAGAT 
CGGGATGATG TGTTACGAAA TGCCATGGTG AATCGGAAGA CTGGGAAATT CTCCATGGAA 
GTGAAGAAGA CTGTGGACAA AGGGAAACGG GTTTTGGTGA TGACCAATGA CTACTACTAC 
ACAGACATCA AGGGTGCTCC TTTCAGTTTA GGTGTGGCGC TCTCCAGGGG CCACGGGAAA 
TACTTCTTCC GAGGGAATGT AACCATTGAA GAAGGGCTCC ATGACTTAGA ACATCCTGAC 
GTGTCCTTGG CAGATGAATG GTCCTACTGC AACACTGATC TGCACCCAGA GCACCGCCAT 
CTCTCTCAAC TGGAAGCGAT TAAGCTCTAC CTCAAAGGCA AGGAGCCTCT GCTTCAATGT 
GACAAAGAAT TGATTCAAGA AGTCCTTTTT GATGCTGTGG TAAGCGCCCC TATCGAAGCC 
TATTGGACCA GCCTGGCCCT CAACAAATCT GAGAATTCTG ACAAGGGTGT AGAGGTCGCC 
TTCCTCGGCA CTCGCACAGG CCTCTCAAGA ATCAACCTGT TTGTGGGGGC TGAACAGCTC 
ACCAATCAGG ACTTTCTGAA GGCTAGAGAC AAAGAGAACA TTTTCAACGC AGATCATTTC 
CCTCTCTGGT ACAGAAGAGC TGCCGAGCAG ATTCCAGGAA GTTTTGTCTA CTCCATCCCC 
TTCAGCACAG GAACGGTCAA CAAAAGCAAT GTGGTGACAG CAAGTACCTC CATCCAACTC 
CTGGATGAGC GAAAATCTCC TGTGGTGGCA GCTGTAGGCA TTCAGATGAA ACTTGAATTC 
TTCCAAAGGA AGTTCTGGAC GGCCAGCAGA CAGTGTGCCT CCCTGGATGG TAAATGCTCC 
ATAAGCTGCG ACGATGAGAC TGTGAACTGT TACCTCATAG ACAATAACGG GTTCATTCTG 
GTGTCTGAAG ACTACACACA GGTGAGTGAG GAGATGCCCA ATGCCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455565.1
CDS	1..2631
Translation	MCRPGSLCCASRGASALLATALLYAALGDVVRSEQQIPLSVVKLWASAFGGEIKSIAAKYSGSQLLQKKYKEYEKDVAIEEIDGLQLVKKLAKNMEEMFHKKSEAVRRLVEAAEEAHLKHEFDADLQYEYFNAVLINERDKDGNFLELGKEFILAPNDHFNNLPVNISLSDVQVPTNMYNKDPAIVNGVYWSESLNKVFVDNFDRDPSLIWQYFGSAKGFFRQYPGIKWEPDENGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIAKQTVSSILDTLGDDDFFNIITYNEELHYVEPCLNGTLVQADRTNKEHFREHLDKLFAKGIGMLDIALNEAFNVLSDFNHTGQGSICSQAIMLITDGAVDTYDTIFAKYNWPERKVRIFTYLIGREAAFADNLKWMACANKGFFTQISTLADVQENVMEYLHVLSRPKVIDQEHDVVWTEAYIDSTLADDQGLVLMTTVAMPVFSKQNETRSKGILLGVVGTDVPVKELLKTIPKYKLGIHGYAFAITNNGYILTHPELRPLYEEGKKRRKPNYSSVDLSEVEWEDRDDVLRNAMVNRKTGKFSMEVKKTVDKGKRVLVMTNDYYYTDIKGAPFSLGVALSRGHGKYFFRGNVTIEEGLHDLEHPDVSLADEWSYCNTDLHPEHRHLSQLEAIKLYLKGKEPLLQCDKELIQEVLFDAVVSAPIEAYWTSLALNKSENSDKGVEVAFLGTRTGLSRINLFVGAEQLTNQDFLKARDKENIFNADHFPLWYRRAAEQIPGSFVYSIPFSTGTVNKSNVVTASTSIQLLDERKSPVVAAVGIQMKLEFFQRKFWTASRQCASLDGKCSISCDDETVNCYLIDNNGFILVSEDYTQVSEEMPNAM

Target ORF information:

RefSeq Version	XM_017600076.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600076.1

ORF Insert Sequence:

ATGTGCCGGC CGGGCTCGCT GTGCTGCGCG TCCCGGGGGG CCTCGGCGCT CCTAGCCACC 
GCGCTTCTCT ACGCCGCGCT GGGGGACGTG GTGCGCTCCG AGCAGCAGAT CCCGCTCTCC 
GTAGTGAAGC TCTGGGCCTC CGCTTTTGGT GGGGAGATAA AATCCATTGC TGCCAAGTAC 
TCGGGTTCCC AGCTTCTGCA AAAGAAATAC AAAGAGTATG AGAAAGACGT TGCCATTGAA 
GAAATCGACG GTCTCCAACT GGTGAAAAAG CTGGCCAAGA ACATGGAAGA GATGTTTCAC 
AAGAAGTCCG AGGCAGTGCG GCGTCTCGTG GAGGCTGCAG AGGAAGCACA CCTGAAGCAT 
GAATTTGACG CCGACTTGCA GTATGAATAC TTCAATGCCG TGCTGATCAA CGAGAGAGAC 
AAAGATGGGA ACTTTTTGGA ATTGGGAAAG GAGTTCATCT TGGCCCCCAA TGACCATTTT 
AATAATTTGC CTGTGAACAT CAGTCTGAGT GATGTCCAAG TGCCAACGAA CATGTACAAC 
AAAGATCCTG CCATAGTCAA TGGAGTGTAT TGGTCTGAAT CTCTAAACAA AGTTTTTGTA 
GACAACTTCG ATCGGGACCC GTCTCTTATA TGGCAGTACT TTGGAAGTGC AAAGGGCTTT 
TTCAGACAGT ACCCAGGGAT TAAATGGGAA CCAGACGAGA ATGGAGTCAT TGCCTTTGAC 
TGCAGGAACA GGAAATGGTA CATCCAGGCA GCAACTTCTC CAAAGGATGT GGTCATTTTG 
GTGGACGTCA GCGGGAGCAT GAAAGGACTC CGCCTGACCA TCGCCAAGCA AACAGTCTCC 
TCGATACTGG ACACCCTGGG CGATGATGAC TTCTTCAACA TCATCACGTA TAATGAAGAG 
CTTCACTATG TGGAACCTTG TCTGAATGGA ACACTGGTTC AAGCGGACAG GACCAACAAG 
GAGCACTTCA GGGAGCATTT GGACAAACTT TTTGCCAAAG GGATTGGAAT GCTCGATATT 
GCGCTGAACG AGGCCTTCAA TGTACTGAGC GATTTCAACC ACACCGGACA AGGAAGCATT 
TGCAGCCAGG CCATTATGCT CATAACCGAC GGGGCAGTGG ACACCTACGA CACCATCTTT 
GCAAAGTACA ATTGGCCAGA GCGAAAGGTT CGAATCTTCA CTTACCTCAT TGGACGAGAG 
GCTGCTTTTG CAGACAATCT CAAGTGGATG GCTTGTGCTA ACAAAGGATT TTTCACCCAG 
ATCTCCACCT TGGCTGATGT GCAGGAAAAT GTCATGGAAT ACCTCCATGT ACTCAGTCGA 
CCCAAAGTCA TCGACCAGGA ACATGATGTG GTGTGGACCG AAGCGTACAT CGACAGCACT 
CTTGCTGATG ATCAGGGCCT CGTCTTGATG ACCACAGTGG CCATGCCTGT GTTTAGTAAG 
CAGAACGAAA CTAGGTCAAA GGGCATTCTT CTGGGTGTGG TTGGCACAGA TGTCCCAGTA 
AAAGAGCTTC TGAAGACCAT CCCCAAATAC AAGTTAGGAA TTCATGGTTA TGCCTTTGCC 
ATCACGAATA ATGGATACAT CTTGACACAC CCGGAGCTCA GGCCCCTGTA TGAAGAAGGG 
AAAAAGCGAA GGAAGCCTAA TTACAGTAGT GTGGATCTCT CGGAAGTCGA GTGGGAAGAT 
CGGGATGATG TGTTACGAAA TGCCATGGTG AATCGGAAGA CTGGGAAATT CTCCATGGAA 
GTGAAGAAGA CTGTGGACAA AGGGAAACGG GTTTTGGTGA TGACCAATGA CTACTACTAC 
ACAGACATCA AGGGTGCTCC TTTCAGTTTA GGTGTGGCGC TCTCCAGGGG CCACGGGAAA 
TACTTCTTCC GAGGGAATGT AACCATTGAA GAAGGGCTCC ATGACTTAGA ACATCCTGAC 
GTGTCCTTGG CAGATGAATG GTCCTACTGC AACACTGATC TGCACCCAGA GCACCGCCAT 
CTCTCTCAAC TGGAAGCGAT TAAGCTCTAC CTCAAAGGCA AGGAGCCTCT GCTTCAATGT 
GACAAAGAAT TGATTCAAGA AGTCCTTTTT GATGCTGTGG TAAGCGCCCC TATCGAAGCC 
TATTGGACCA GCCTGGCCCT CAACAAATCT GAGAATTCTG ACAAGGGTGT AGAGGTCGCC 
TTCCTCGGCA CTCGCACAGG CCTCTCAAGA ATCAACCTGT TTGTGGGGGC TGAACAGCTC 
ACCAATCAGG ACTTTCTGAA GGCTAGAGAC AAAGAGAACA TTTTCAACGC AGATCATTTC 
CCTCTCTGGT ACAGAAGAGC TGCCGAGCAG ATTCCAGGAA GTTTTGTCTA CTCCATCCCC 
TTCAGCACAG GAACGGTCAA CAAAAGCAAT GTGGTGACAG CAAGTACCTC CATCCAACTC 
CTGGATGAGC GAAAATCTCC TGTGGTGGCA GCTGTAGGCA TTCAGATGAA ACTTGAATTC 
TTCCAAAGGA AGTTCTGGAC GGCCAGCAGA CAGTGTGCCT CCCTGGATGG TAAATGCTCC 
ATAAGCTGCG ACGATGAGAC TGTGAACTGT TACCTCATAG ACAATAACGG GTTCATTCTG 
GTGTCTGAAG ACTACACACA GGTGAGTGAG GAGATGCCCA ATGCCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d3 ( NM_175595.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_783185.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), mRNA.

Price & Availability↑

CloneID	ORa03862
Clone ID Related Accession (Same CDS sequence)	NM_175595.2
Accession Version	NM_175595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF486278.1. On Jun 3, 2003 this sequence version replaced NM_175595.1. ##Evidence-Data-START## Transcript exon combination :: AF486278.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGGC CGGGCTCGCT GTGCTGCGCG TCCCGGGGGG CCTCGGCGCT CCTAGCCACA 
GCGCTTCTCT ACGCCGCGCT GGGGGACGTG GTGCGCTCCG AGCAGCAGAT CCCGCTCTCC 
GTAGTGAAGC TCTGGGCCTC CGCTTTTGGT GGGGAGATAA AATCCATTGC TGCCAAGTAC 
TCGGGTTCCC AGCTTCTGCA AAAGAAATAC AAAGAGTATG AGAAAGACGT TGCCATTGAA 
GAAATCGACG GTCTCCAACT GGTGAAAAAG CTGGCCAAGA ACATGGAAGA GATGTTTCAC 
AAGAAGTCCG AGGCAGTGCG GCGTCTCGTG GAGGCTGCAG AGGAAGCACA CCTGAAGCAT 
GAATTTGACG CCGACTTGCA GTATGAATAC TTCAATGCCG TGCTGATCAA CGAGAGAGAC 
AAAGATGGGA ACTTTTTGGA ATTGGGAAAG GAGTTCATCT TGGCCCCCAA TGACCATTTT 
AATAATTTGC CTGTGAACAT CAGTCTGAGT GATGTCCAAG TGCCAACGAA CATGTACAAC 
AAAGATCCTG CCATAGTCAA TGGAGTGTAT TGGTCTGAAT CTCTAAACAA AGTTTTTGTA 
GACAACTTCG ATCGGGACCC GTCTCTCATA TGGCAGTACT TTGGAAGTGC AAAGGGCTTT 
TTCAGACAGT ACCCAGGGAT TAAATGGGAA CCAGACGAGA ATGGAGTCAT TGCCTTTGAC 
TGCAGGAACA GGAAATGGTA CATCCAGGCA GCAACTTCTC CAAAGGATGT GGTCATTTTG 
GTGGACGTCA GCGGGAGCAT GAAAGGACTC CGCCTGACCA TCGCCAAGCA AACAGTCTCC 
TCGATACTGG ACACCCTGGG CGATGATGAC TTCTTCAACA TCATCACGTA TAACGAAGAG 
CTTCACTATG TGGAACCTTG TCTGAATGGA ACACTGGTTC AAGCGGACAG GACCAACAAG 
GAGCACTTCA GGGAGCATTT GGACAAACTT TTTGCCAAAG GGATTGGAAT GCTCGATATT 
GCGCTGAACG AGGCCTTCAA TGTACTGAGC GATTTCAACC ACACCGGACA AGGAAGCATT 
TGCAGCCAGG CCATTATGCT CATAACCGAC GGGGCAGTGG ACACCTACGA CACCATCTTT 
GCAAAGTACA ATTGGCCAGA GCGAAAGGTT CGAATCTTCA CTTACCTCAT TGGACGAGAG 
GCTGCTTTTG CAGACAATCT CAAGTGGATG GCTTGTGCTA ACAAAGGATT TTTCACCCAG 
ATCTCCACCT TGGCTGATGT GCAGGAAAAT GTCATGGAAT ACCTCCATGT ACTCAGTCGA 
CCCAAAGTCA TCGACCAGGA ACATGATGTG GTGTGGACCG AAGCGTACAT CGACAGCACT 
CTTGCTGATG ATCAGGGCCT CGTCTTGATG ACCACAGTGG CCATGCCTGT GTTTAGTAAG 
CAGAACGAAA CTAGGTCAAA GGGCATTCTT CTGGGTGTGG TTGGCACAGA TGTCCCAGTA 
AAAGAGCTTC TGAAGACCAT CCCCAAATAC AAGTTAGGAA TTCATGGTTA TGCCTTTGCC 
ATCACGAATA ATGGATACAT CTTGACACAC CCGGAGCTCA GGCCCCTGTA TGAAGAAGGG 
AAAAAGCGAA GGAAGCCTAA TTACAGTAGT GTGGATCTCT CGGAAGTCGA GTGGGAAGAT 
CGGGATGATG TGTTACGAAA TGCCATGGTG AATCGGAAGA CTGGGAAATT CTCCATGGAA 
GTGAAGAAGA CTGTGGACAA AGGGAAACGG GTTTTGGTGA TGACCAATGA CTACTACTAC 
ACAGACATCA AGGGTGCTCC TTTCAGTTTA GGTGTGGCGC TCTCCAGGGG CCACGGGAAA 
TACTTCTTCC GAGGGAATGT AACCATTGAA GAAGGGCTCC ATGACTTAGA ACATCCTGAC 
GTGTCCTTGG CAGATGAATG GTCCTACTGC AACACTGATC TGCACCCAGA GCACCGCCAT 
CTCTCTCAAC TGGAAGCGAT TAAGCTCTAC CTCAAAGGCA AGGAGCCTCT GCTTCAATGT 
GACAAAGAAT TGATTCAAGA AGTCCTTTTT GATGCTGTGG TAAGCGCCCC TATCGAAGCC 
TATTGGACCA GCCTGGCCCT CAACAAATCT GAGAATTCTG ACAAGGGTGT AGAGGTCGCC 
TTCCTCGGCA CTCGCACAGG CCTCTCAAGA ATCAACCTGT TTGTGGGGGC TGAACAGCTC 
ACCAATCAGG ACTTTCTGAA GGCTAGAGAC AAAGAGAACA TTTTCAACGC AGATCATTTC 
CCTCTCTGGT ACAGAAGAGC TGCCGAGCAG ATTCCAGGAA GTTTTGTCTA CTCCATCCCC 
TTCAGCACAG GAACGGTCAA CAAAAGCAAT GTGGTGACAG CAAGTACCTC CATCCAACTC 
CTGGATGAGC GAAAATCTCC TGTGGTGGCA GCTGTAGGCA TTCAGATGAA ACTTGAATTC 
TTCCAAAGGA AGTTCTGGAC GGCCAGCAGA CAGTGTGCCT CCCTGGATGG TAAATGCTCC 
ATAAGCTGCG ACGATGAGAC TGTGAACTGT TACCTCATAG ACAATAACGG GTTCATTCTG 
GTGTCTGAAG ACTACACACA GACTGGAGAT TTTTTTGGTG AGGTCGAAGG AGCTGTCATG 
AACAAGTTGT TAACAATGGG CTCCTTTAAA AGAATAACCT TGTATGACTA CCAAGCCATG 
TGTAGAGCCA ACAAGGAGAG TAGTGACAGT GCCCACGGAC TCCTGGACCC CTATAAGGCC 
TTCCTCTCTG CAGCCAAGTG GATAGTGACG GAACTTGTCT TGTTCCTGGT GGAGTTTAAC 
CTTTGCAGTT GGTGGCACTC TGACATGACA GCTAAAGCCC AGAAACTGAA ACAGACCCTG 
GAGCCTTGTG ATACTGAATA CCCAGCCTTT GTTTCCGAAC GCACCATCAA GGAGACCACA 
GGGAACATTG CTTGTGAAGA CTGCTCCAAG TCCTTTGTCA TCCAGCAAAT CCCAAGTAGC 
AATCTGTTCA TGGTGGTGGT GGACAGTAGC TGTCTCTGTG AGTCTGTGGC TCCTATCACC 
ATGGCACCCA TTGAAATCAG GTATAATGAA TCCCTTAAGT GTGAACGGTT AAAGGCTCAG 
AAGATCAGAC GACGTCCGGA ATCCTGCCAC GGCTTCCATC CTGAGGAGAA TGCGAGAGAG 
TGTGGGGGTG CATCAAGTCT CCAGGCCCAG GTGGCCTTGC TGCTGCTCCC CCTGGTTTCG 
AGTCTCTTCT CAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783185.1
CDS	1..3258
Translation	MAGPGSLCCASRGASALLATALLYAALGDVVRSEQQIPLSVVKLWASAFGGEIKSIAAKYSGSQLLQKKYKEYEKDVAIEEIDGLQLVKKLAKNMEEMFHKKSEAVRRLVEAAEEAHLKHEFDADLQYEYFNAVLINERDKDGNFLELGKEFILAPNDHFNNLPVNISLSDVQVPTNMYNKDPAIVNGVYWSESLNKVFVDNFDRDPSLIWQYFGSAKGFFRQYPGIKWEPDENGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIAKQTVSSILDTLGDDDFFNIITYNEELHYVEPCLNGTLVQADRTNKEHFREHLDKLFAKGIGMLDIALNEAFNVLSDFNHTGQGSICSQAIMLITDGAVDTYDTIFAKYNWPERKVRIFTYLIGREAAFADNLKWMACANKGFFTQISTLADVQENVMEYLHVLSRPKVIDQEHDVVWTEAYIDSTLADDQGLVLMTTVAMPVFSKQNETRSKGILLGVVGTDVPVKELLKTIPKYKLGIHGYAFAITNNGYILTHPELRPLYEEGKKRRKPNYSSVDLSEVEWEDRDDVLRNAMVNRKTGKFSMEVKKTVDKGKRVLVMTNDYYYTDIKGAPFSLGVALSRGHGKYFFRGNVTIEEGLHDLEHPDVSLADEWSYCNTDLHPEHRHLSQLEAIKLYLKGKEPLLQCDKELIQEVLFDAVVSAPIEAYWTSLALNKSENSDKGVEVAFLGTRTGLSRINLFVGAEQLTNQDFLKARDKENIFNADHFPLWYRRAAEQIPGSFVYSIPFSTGTVNKSNVVTASTSIQLLDERKSPVVAAVGIQMKLEFFQRKFWTASRQCASLDGKCSISCDDETVNCYLIDNNGFILVSEDYTQTGDFFGEVEGAVMNKLLTMGSFKRITLYDYQAMCRANKESSDSAHGLLDPYKAFLSAAKWIVTELVLFLVEFNLCSWWHSDMTAKAQKLKQTLEPCDTEYPAFVSERTIKETTGNIACEDCSKSFVIQQIPSSNLFMVVVDSSCLCESVAPITMAPIEIRYNESLKCERLKAQKIRRRPESCHGFHPEENARECGGASSLQAQVALLLLPLVSSLFSR

Target ORF information:

RefSeq Version	NM_175595.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175595.2

ORF Insert Sequence:

ATGGCCGGGC CGGGCTCGCT GTGCTGCGCG TCCCGGGGGG CCTCGGCGCT CCTAGCCACA 
GCGCTTCTCT ACGCCGCGCT GGGGGACGTG GTGCGCTCCG AGCAGCAGAT CCCGCTCTCC 
GTAGTGAAGC TCTGGGCCTC CGCTTTTGGT GGGGAGATAA AATCCATTGC TGCCAAGTAC 
TCGGGTTCCC AGCTTCTGCA AAAGAAATAC AAAGAGTATG AGAAAGACGT TGCCATTGAA 
GAAATCGACG GTCTCCAACT GGTGAAAAAG CTGGCCAAGA ACATGGAAGA GATGTTTCAC 
AAGAAGTCCG AGGCAGTGCG GCGTCTCGTG GAGGCTGCAG AGGAAGCACA CCTGAAGCAT 
GAATTTGACG CCGACTTGCA GTATGAATAC TTCAATGCCG TGCTGATCAA CGAGAGAGAC 
AAAGATGGGA ACTTTTTGGA ATTGGGAAAG GAGTTCATCT TGGCCCCCAA TGACCATTTT 
AATAATTTGC CTGTGAACAT CAGTCTGAGT GATGTCCAAG TGCCAACGAA CATGTACAAC 
AAAGATCCTG CCATAGTCAA TGGAGTGTAT TGGTCTGAAT CTCTAAACAA AGTTTTTGTA 
GACAACTTCG ATCGGGACCC GTCTCTCATA TGGCAGTACT TTGGAAGTGC AAAGGGCTTT 
TTCAGACAGT ACCCAGGGAT TAAATGGGAA CCAGACGAGA ATGGAGTCAT TGCCTTTGAC 
TGCAGGAACA GGAAATGGTA CATCCAGGCA GCAACTTCTC CAAAGGATGT GGTCATTTTG 
GTGGACGTCA GCGGGAGCAT GAAAGGACTC CGCCTGACCA TCGCCAAGCA AACAGTCTCC 
TCGATACTGG ACACCCTGGG CGATGATGAC TTCTTCAACA TCATCACGTA TAACGAAGAG 
CTTCACTATG TGGAACCTTG TCTGAATGGA ACACTGGTTC AAGCGGACAG GACCAACAAG 
GAGCACTTCA GGGAGCATTT GGACAAACTT TTTGCCAAAG GGATTGGAAT GCTCGATATT 
GCGCTGAACG AGGCCTTCAA TGTACTGAGC GATTTCAACC ACACCGGACA AGGAAGCATT 
TGCAGCCAGG CCATTATGCT CATAACCGAC GGGGCAGTGG ACACCTACGA CACCATCTTT 
GCAAAGTACA ATTGGCCAGA GCGAAAGGTT CGAATCTTCA CTTACCTCAT TGGACGAGAG 
GCTGCTTTTG CAGACAATCT CAAGTGGATG GCTTGTGCTA ACAAAGGATT TTTCACCCAG 
ATCTCCACCT TGGCTGATGT GCAGGAAAAT GTCATGGAAT ACCTCCATGT ACTCAGTCGA 
CCCAAAGTCA TCGACCAGGA ACATGATGTG GTGTGGACCG AAGCGTACAT CGACAGCACT 
CTTGCTGATG ATCAGGGCCT CGTCTTGATG ACCACAGTGG CCATGCCTGT GTTTAGTAAG 
CAGAACGAAA CTAGGTCAAA GGGCATTCTT CTGGGTGTGG TTGGCACAGA TGTCCCAGTA 
AAAGAGCTTC TGAAGACCAT CCCCAAATAC AAGTTAGGAA TTCATGGTTA TGCCTTTGCC 
ATCACGAATA ATGGATACAT CTTGACACAC CCGGAGCTCA GGCCCCTGTA TGAAGAAGGG 
AAAAAGCGAA GGAAGCCTAA TTACAGTAGT GTGGATCTCT CGGAAGTCGA GTGGGAAGAT 
CGGGATGATG TGTTACGAAA TGCCATGGTG AATCGGAAGA CTGGGAAATT CTCCATGGAA 
GTGAAGAAGA CTGTGGACAA AGGGAAACGG GTTTTGGTGA TGACCAATGA CTACTACTAC 
ACAGACATCA AGGGTGCTCC TTTCAGTTTA GGTGTGGCGC TCTCCAGGGG CCACGGGAAA 
TACTTCTTCC GAGGGAATGT AACCATTGAA GAAGGGCTCC ATGACTTAGA ACATCCTGAC 
GTGTCCTTGG CAGATGAATG GTCCTACTGC AACACTGATC TGCACCCAGA GCACCGCCAT 
CTCTCTCAAC TGGAAGCGAT TAAGCTCTAC CTCAAAGGCA AGGAGCCTCT GCTTCAATGT 
GACAAAGAAT TGATTCAAGA AGTCCTTTTT GATGCTGTGG TAAGCGCCCC TATCGAAGCC 
TATTGGACCA GCCTGGCCCT CAACAAATCT GAGAATTCTG ACAAGGGTGT AGAGGTCGCC 
TTCCTCGGCA CTCGCACAGG CCTCTCAAGA ATCAACCTGT TTGTGGGGGC TGAACAGCTC 
ACCAATCAGG ACTTTCTGAA GGCTAGAGAC AAAGAGAACA TTTTCAACGC AGATCATTTC 
CCTCTCTGGT ACAGAAGAGC TGCCGAGCAG ATTCCAGGAA GTTTTGTCTA CTCCATCCCC 
TTCAGCACAG GAACGGTCAA CAAAAGCAAT GTGGTGACAG CAAGTACCTC CATCCAACTC 
CTGGATGAGC GAAAATCTCC TGTGGTGGCA GCTGTAGGCA TTCAGATGAA ACTTGAATTC 
TTCCAAAGGA AGTTCTGGAC GGCCAGCAGA CAGTGTGCCT CCCTGGATGG TAAATGCTCC 
ATAAGCTGCG ACGATGAGAC TGTGAACTGT TACCTCATAG ACAATAACGG GTTCATTCTG 
GTGTCTGAAG ACTACACACA GACTGGAGAT TTTTTTGGTG AGGTCGAAGG AGCTGTCATG 
AACAAGTTGT TAACAATGGG CTCCTTTAAA AGAATAACCT TGTATGACTA CCAAGCCATG 
TGTAGAGCCA ACAAGGAGAG TAGTGACAGT GCCCACGGAC TCCTGGACCC CTATAAGGCC 
TTCCTCTCTG CAGCCAAGTG GATAGTGACG GAACTTGTCT TGTTCCTGGT GGAGTTTAAC 
CTTTGCAGTT GGTGGCACTC TGACATGACA GCTAAAGCCC AGAAACTGAA ACAGACCCTG 
GAGCCTTGTG ATACTGAATA CCCAGCCTTT GTTTCCGAAC GCACCATCAA GGAGACCACA 
GGGAACATTG CTTGTGAAGA CTGCTCCAAG TCCTTTGTCA TCCAGCAAAT CCCAAGTAGC 
AATCTGTTCA TGGTGGTGGT GGACAGTAGC TGTCTCTGTG AGTCTGTGGC TCCTATCACC 
ATGGCACCCA TTGAAATCAG GTATAATGAA TCCCTTAAGT GTGAACGGTT AAAGGCTCAG 
AAGATCAGAC GACGTCCGGA ATCCTGCCAC GGCTTCCATC CTGAGGAGAA TGCGAGAGAG 
TGTGGGGGTG CATCAAGTCT CCAGGCCCAG GTGGCCTTGC TGCTGCTCCC CCTGGTTTCG 
AGTCTCTTCT CAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d3 ( XM_017600077.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455566.1 Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), transcript variant X2, mRNA.

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CloneID	ORa58797
Clone ID Related Accession (Same CDS sequence)	XM_017600077.1
Accession Version	XM_017600077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCGGC CGGGCTCGCT GTGCTGCGCG TCCCGGGGGG CCTCGGCGCT CCTAGCCACC 
GCGCTTCTCT ACGCCGCGCT GGGGGACGTG GTGCGCTCCG AGCAGCAGAT CCCGCTCTCC 
GTAGTGAAGC TCTGGGCCTC CGCTTTTGGT GGGGAGATAA AATCCATTGC TGCCAAGTAC 
TCGGGTTCCC AGCTTCTGCA AAAGAAATAC AAAGAGTATG AGAAAGACGT TGCCATTGAA 
GAAATCGACG GTCTCCAACT GGTGAAAAAG CTGGCCAAGA ACATGGAAGA GATGTTTCAC 
AAGAAGTCCG AGGCAGTGCG GCGTCTCGTG GAGGCTGCAG AGGAAGCACA CCTGAAGCAT 
GAATTTGACG CCGACTTGCA GTATGAATAC TTCAATGCCG TGCTGATCAA CGAGAGAGAC 
AAAGATGGGA ACTTTTTGGA ATTGGGAAAG GAGTTCATCT TGGCCCCCAA TGACCATTTT 
AATAATTTGC CTGTGAACAT CAGTCTGAGT GATGTCCAAG TGCCAACGAA CATGTACAAC 
AAAGATCCTG CCATAGTCAA TGGAGTGTAT TGGTCTGAAT CTCTAAACAA AGTTTTTGTA 
GACAACTTCG ATCGGGACCC GTCTCTTATA TGGCAGTACT TTGGAAGTGC AAAGGGCTTT 
TTCAGACAGT ACCCAGGGAT TAAATGGGAA CCAGACGAGA ATGGAGTCAT TGCCTTTGAC 
TGCAGGAACA GGAAATGGTA CATCCAGGCA GCAACTTCTC CAAAGGATGT GGTCATTTTG 
GTGGACGTCA GCGGGAGCAT GAAAGGACTC CGCCTGACCA TCGCCAAGCA AACAGTCTCC 
TCGATACTGG ACACCCTGGG CGATGATGAC TTCTTCAACA TCATCACGTA TAATGAAGAG 
CTTCACTATG TGGAACCTTG TCTGAATGGA ACACTGGTTC AAGCGGACAG GACCAACAAG 
GAGCACTTCA GGGAGCATTT GGACAAACTT TTTGCCAAAG GGATTGGAAT GCTCGATATT 
GCGCTGAACG AGGCCTTCAA TGTACTGAGC GATTTCAACC ACACCGGACA AGGAAGCATT 
TGCAGCCAGG CCATTATGCT CATAACCGAC GGGGCAGTGG ACACCTACGA CACCATCTTT 
GCAAAGTACA ATTGGCCAGA GCGAAAGGTT CGAATCTTCA CTTACCTCAT TGGACGAGAG 
GCTGCTTTTG CAGACAATCT CAAGTGGATG GCTTGTGCTA ACAAAGGATT TTTCACCCAG 
ATCTCCACCT TGGCTGATGT GCAGGAAAAT GTCATGGAAT ACCTCCATGT ACTCAGTCGA 
CCCAAAGTCA TCGACCAGGA ACATGATGTG GTGTGGACCG AAGCGTACAT CGACAGCACT 
CTCCCTCAGG CTCAAAAGCT TGCTGATGAT CAGGGCCTCG TCTTGATGAC CACAGTGGCC 
ATGCCTGTGT TTAGTAAGCA GAACGAAACT AGGTCAAAGG GCATTCTTCT GGGTGTGGTT 
GGCACAGATG TCCCAGTAAA AGAGCTTCTG AAGACCATCC CCAAATACAA GTTAGGAATT 
CATGGTTATG CCTTTGCCAT CACGAATAAT GGATACATCT TGACACACCC GGAGCTCAGG 
CCCCTGTATG AAGAAGGGAA AAAGCGAAGG AAGCCTAATT ACAGTAGTGT GGATCTCTCG 
GAAGTCGAGT GGGAAGATCG GGATGATGTG TTACGAAATG CCATGGTGAA TCGGAAGACT 
GGGAAATTCT CCATGGAAGT GAAGAAGACT GTGGACAAAG GGAAACGGGT TTTGGTGATG 
ACCAATGACT ACTACTACAC AGACATCAAG GGTGCTCCTT TCAGTTTAGG TGTGGCGCTC 
TCCAGGGGCC ACGGGAAATA CTTCTTCCGA GGGAATGTAA CCATTGAAGA AGGGCTCCAT 
GACTTAGAAC ATCCTGACGT GTCCTTGGCA GATGAATGGT CCTACTGCAA CACTGATCTG 
CACCCAGAGC ACCGCCATCT CTCTCAACTG GAAGCGATTA AGCTCTACCT CAAAGGCAAG 
GAGCCTCTGC TTCAATGTGA CAAAGAATTG ATTCAAGAAG TCCTTTTTGA TGCTGTGGTA 
AGCGCCCCTA TCGAAGCCTA TTGGACCAGC CTGGCCCTCA ACAAATCTGA GAATTCTGAC 
AAGGGTGTAG AGGTCGCCTT CCTCGGCACT CGCACAGGCC TCTCAAGAAT CAACCTGTTT 
GTGGGGGCTG AACAGCTCAC CAATCAGGAC TTTCTGAAGG CTAGAGACAA AGAGAACATT 
TTCAACGCAG ATCATTTCCC TCTCTGGTAC AGAAGAGCTG CCGAGCAGAT TCCAGGAAGT 
TTTGTCTACT CCATCCCCTT CAGCACAGGA ACGGTCAACA AAAGCAATGT GGTGACAGCA 
AGTACCTCCA TCCAACTCCT GGATGAGCGA AAATCTCCTG TGGTGGCAGC TGTAGGCATT 
CAGATGAAAC TTGAATTCTT CCAAAGGAAG TTCTGGACGG CCAGCAGACA GTGTGCCTCC 
CTGGATGGTA AATGCTCCAT AAGCTGCGAC GATGAGACTG TGAACTGTTA CCTCATAGAC 
AATAACGGGT TCATTCTGGT GTCTGAAGAC TACACACAGA CTGGAGATTT TTTTGGTGAG 
GTCGAAGGAG CTGTCATGAA CAAGTTGTTA ACAATGGGCT CCTTTAAAAG AATAACCTTG 
TATGACTACC AAGCCATGTG TAGAGCCAAC AAGGAGAGTA GTGACAGTGC CCACGGACTC 
CTGGACCCCT ATAAGGCCTT CCTCTCTGCA GCCAAGTGGA TAGTGACGGA ACTTGTCTTG 
TTCCTGGTGG AGTTTAACCT TTGCAGTTGG TGGCACTCTG ACATGACAGC TAAAGCCCAG 
AAACTGAAAC AGACCCTGGA GCCTTGTGAT ACTGAATACC CAGCCTTTGT TTCCGAACGC 
ACCATCAAGG AGACCACAGG GAACATTGCT TGTGAAGACT GCTCCAAGTC CTTTGTCATC 
CAGCAAATCC CAAGTAGCAA TCTGTTCATG GTGGTGGTGG ACAGTAGCTG TCTCTGTGAG 
TCTGTGGCTC CTATCACCAT GGCACCCATT GAAATCAGGT ATAATGAATC CCTTAAGTGT 
GAACGGTTAA AGGCTCAGAA GATCAGACGA CGTCCGGAAT CCTGCCACGG CTTCCATCCT 
GAGGAGAATG CGAGAGAGTG TGGGGGTGCA TCAAGTCTCC AGGCCCAGGT GGCCTTGCTG 
CTGCTCCCCC TGGTTTCGAG TCTCTTCTCA AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455566.1
CDS	1..3276
Translation	MCRPGSLCCASRGASALLATALLYAALGDVVRSEQQIPLSVVKLWASAFGGEIKSIAAKYSGSQLLQKKYKEYEKDVAIEEIDGLQLVKKLAKNMEEMFHKKSEAVRRLVEAAEEAHLKHEFDADLQYEYFNAVLINERDKDGNFLELGKEFILAPNDHFNNLPVNISLSDVQVPTNMYNKDPAIVNGVYWSESLNKVFVDNFDRDPSLIWQYFGSAKGFFRQYPGIKWEPDENGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIAKQTVSSILDTLGDDDFFNIITYNEELHYVEPCLNGTLVQADRTNKEHFREHLDKLFAKGIGMLDIALNEAFNVLSDFNHTGQGSICSQAIMLITDGAVDTYDTIFAKYNWPERKVRIFTYLIGREAAFADNLKWMACANKGFFTQISTLADVQENVMEYLHVLSRPKVIDQEHDVVWTEAYIDSTLPQAQKLADDQGLVLMTTVAMPVFSKQNETRSKGILLGVVGTDVPVKELLKTIPKYKLGIHGYAFAITNNGYILTHPELRPLYEEGKKRRKPNYSSVDLSEVEWEDRDDVLRNAMVNRKTGKFSMEVKKTVDKGKRVLVMTNDYYYTDIKGAPFSLGVALSRGHGKYFFRGNVTIEEGLHDLEHPDVSLADEWSYCNTDLHPEHRHLSQLEAIKLYLKGKEPLLQCDKELIQEVLFDAVVSAPIEAYWTSLALNKSENSDKGVEVAFLGTRTGLSRINLFVGAEQLTNQDFLKARDKENIFNADHFPLWYRRAAEQIPGSFVYSIPFSTGTVNKSNVVTASTSIQLLDERKSPVVAAVGIQMKLEFFQRKFWTASRQCASLDGKCSISCDDETVNCYLIDNNGFILVSEDYTQTGDFFGEVEGAVMNKLLTMGSFKRITLYDYQAMCRANKESSDSAHGLLDPYKAFLSAAKWIVTELVLFLVEFNLCSWWHSDMTAKAQKLKQTLEPCDTEYPAFVSERTIKETTGNIACEDCSKSFVIQQIPSSNLFMVVVDSSCLCESVAPITMAPIEIRYNESLKCERLKAQKIRRRPESCHGFHPEENARECGGASSLQAQVALLLLPLVSSLFSR

Target ORF information:

RefSeq Version	XM_017600077.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calcium voltage-gated channel auxiliary subunit alpha2delta 3 (Cacna2d3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600077.1

ORF Insert Sequence:

ATGTGCCGGC CGGGCTCGCT GTGCTGCGCG TCCCGGGGGG CCTCGGCGCT CCTAGCCACC 
GCGCTTCTCT ACGCCGCGCT GGGGGACGTG GTGCGCTCCG AGCAGCAGAT CCCGCTCTCC 
GTAGTGAAGC TCTGGGCCTC CGCTTTTGGT GGGGAGATAA AATCCATTGC TGCCAAGTAC 
TCGGGTTCCC AGCTTCTGCA AAAGAAATAC AAAGAGTATG AGAAAGACGT TGCCATTGAA 
GAAATCGACG GTCTCCAACT GGTGAAAAAG CTGGCCAAGA ACATGGAAGA GATGTTTCAC 
AAGAAGTCCG AGGCAGTGCG GCGTCTCGTG GAGGCTGCAG AGGAAGCACA CCTGAAGCAT 
GAATTTGACG CCGACTTGCA GTATGAATAC TTCAATGCCG TGCTGATCAA CGAGAGAGAC 
AAAGATGGGA ACTTTTTGGA ATTGGGAAAG GAGTTCATCT TGGCCCCCAA TGACCATTTT 
AATAATTTGC CTGTGAACAT CAGTCTGAGT GATGTCCAAG TGCCAACGAA CATGTACAAC 
AAAGATCCTG CCATAGTCAA TGGAGTGTAT TGGTCTGAAT CTCTAAACAA AGTTTTTGTA 
GACAACTTCG ATCGGGACCC GTCTCTTATA TGGCAGTACT TTGGAAGTGC AAAGGGCTTT 
TTCAGACAGT ACCCAGGGAT TAAATGGGAA CCAGACGAGA ATGGAGTCAT TGCCTTTGAC 
TGCAGGAACA GGAAATGGTA CATCCAGGCA GCAACTTCTC CAAAGGATGT GGTCATTTTG 
GTGGACGTCA GCGGGAGCAT GAAAGGACTC CGCCTGACCA TCGCCAAGCA AACAGTCTCC 
TCGATACTGG ACACCCTGGG CGATGATGAC TTCTTCAACA TCATCACGTA TAATGAAGAG 
CTTCACTATG TGGAACCTTG TCTGAATGGA ACACTGGTTC AAGCGGACAG GACCAACAAG 
GAGCACTTCA GGGAGCATTT GGACAAACTT TTTGCCAAAG GGATTGGAAT GCTCGATATT 
GCGCTGAACG AGGCCTTCAA TGTACTGAGC GATTTCAACC ACACCGGACA AGGAAGCATT 
TGCAGCCAGG CCATTATGCT CATAACCGAC GGGGCAGTGG ACACCTACGA CACCATCTTT 
GCAAAGTACA ATTGGCCAGA GCGAAAGGTT CGAATCTTCA CTTACCTCAT TGGACGAGAG 
GCTGCTTTTG CAGACAATCT CAAGTGGATG GCTTGTGCTA ACAAAGGATT TTTCACCCAG 
ATCTCCACCT TGGCTGATGT GCAGGAAAAT GTCATGGAAT ACCTCCATGT ACTCAGTCGA 
CCCAAAGTCA TCGACCAGGA ACATGATGTG GTGTGGACCG AAGCGTACAT CGACAGCACT 
CTCCCTCAGG CTCAAAAGCT TGCTGATGAT CAGGGCCTCG TCTTGATGAC CACAGTGGCC 
ATGCCTGTGT TTAGTAAGCA GAACGAAACT AGGTCAAAGG GCATTCTTCT GGGTGTGGTT 
GGCACAGATG TCCCAGTAAA AGAGCTTCTG AAGACCATCC CCAAATACAA GTTAGGAATT 
CATGGTTATG CCTTTGCCAT CACGAATAAT GGATACATCT TGACACACCC GGAGCTCAGG 
CCCCTGTATG AAGAAGGGAA AAAGCGAAGG AAGCCTAATT ACAGTAGTGT GGATCTCTCG 
GAAGTCGAGT GGGAAGATCG GGATGATGTG TTACGAAATG CCATGGTGAA TCGGAAGACT 
GGGAAATTCT CCATGGAAGT GAAGAAGACT GTGGACAAAG GGAAACGGGT TTTGGTGATG 
ACCAATGACT ACTACTACAC AGACATCAAG GGTGCTCCTT TCAGTTTAGG TGTGGCGCTC 
TCCAGGGGCC ACGGGAAATA CTTCTTCCGA GGGAATGTAA CCATTGAAGA AGGGCTCCAT 
GACTTAGAAC ATCCTGACGT GTCCTTGGCA GATGAATGGT CCTACTGCAA CACTGATCTG 
CACCCAGAGC ACCGCCATCT CTCTCAACTG GAAGCGATTA AGCTCTACCT CAAAGGCAAG 
GAGCCTCTGC TTCAATGTGA CAAAGAATTG ATTCAAGAAG TCCTTTTTGA TGCTGTGGTA 
AGCGCCCCTA TCGAAGCCTA TTGGACCAGC CTGGCCCTCA ACAAATCTGA GAATTCTGAC 
AAGGGTGTAG AGGTCGCCTT CCTCGGCACT CGCACAGGCC TCTCAAGAAT CAACCTGTTT 
GTGGGGGCTG AACAGCTCAC CAATCAGGAC TTTCTGAAGG CTAGAGACAA AGAGAACATT 
TTCAACGCAG ATCATTTCCC TCTCTGGTAC AGAAGAGCTG CCGAGCAGAT TCCAGGAAGT 
TTTGTCTACT CCATCCCCTT CAGCACAGGA ACGGTCAACA AAAGCAATGT GGTGACAGCA 
AGTACCTCCA TCCAACTCCT GGATGAGCGA AAATCTCCTG TGGTGGCAGC TGTAGGCATT 
CAGATGAAAC TTGAATTCTT CCAAAGGAAG TTCTGGACGG CCAGCAGACA GTGTGCCTCC 
CTGGATGGTA AATGCTCCAT AAGCTGCGAC GATGAGACTG TGAACTGTTA CCTCATAGAC 
AATAACGGGT TCATTCTGGT GTCTGAAGAC TACACACAGA CTGGAGATTT TTTTGGTGAG 
GTCGAAGGAG CTGTCATGAA CAAGTTGTTA ACAATGGGCT CCTTTAAAAG AATAACCTTG 
TATGACTACC AAGCCATGTG TAGAGCCAAC AAGGAGAGTA GTGACAGTGC CCACGGACTC 
CTGGACCCCT ATAAGGCCTT CCTCTCTGCA GCCAAGTGGA TAGTGACGGA ACTTGTCTTG 
TTCCTGGTGG AGTTTAACCT TTGCAGTTGG TGGCACTCTG ACATGACAGC TAAAGCCCAG 
AAACTGAAAC AGACCCTGGA GCCTTGTGAT ACTGAATACC CAGCCTTTGT TTCCGAACGC 
ACCATCAAGG AGACCACAGG GAACATTGCT TGTGAAGACT GCTCCAAGTC CTTTGTCATC 
CAGCAAATCC CAAGTAGCAA TCTGTTCATG GTGGTGGTGG ACAGTAGCTG TCTCTGTGAG 
TCTGTGGCTC CTATCACCAT GGCACCCATT GAAATCAGGT ATAATGAATC CCTTAAGTGT 
GAACGGTTAA AGGCTCAGAA GATCAGACGA CGTCCGGAAT CCTGCCACGG CTTCCATCCT 
GAGGAGAATG CGAGAGAGTG TGGGGGTGCA TCAAGTCTCC AGGCCCAGGT GGCCTTGCTG 
CTGCTCCCCC TGGTTTCGAG TCTCTTCTCA AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina.
The Journal of comparative neurology523(10)1443-60(2015 Jul)
Pérez de Sevilla Müller L,Sargoy A,Fernández-Sánchez L,Rodriguez A,Liu J,Cuenca N,Brecha N

The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for function.
Proceedings of the National Academy of Sciences of the United States of America107(4)1654-9(2010 Jan)
Davies A,Kadurin I,Alvarez-Laviada A,Douglas L,Nieto-Rostro M,Bauer CS,Pratt WS,Dolphin AC

Molecular cloning of calcium channel alpha(2)delta-subunits from rat atria and the differential regulation of their expression by IGF-1.
Journal of molecular and cellular cardiology35(2)207-15(2003 Feb)
Chu PJ,Best PM

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Cacna2d3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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