Ndc80 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndc80
Entrez Gene ID	301701
Full Name	NDC80 kinetochore complex component
Synonyms	Kntc2
General protein information	Preferred Names NDC80 kinetochore complex component Names kinetochore protein NDC80 homolog NDC80 homolog, kinetochore complex component kinetochore associated 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q38

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767423.1	XP_008765645.1	kinetochore protein NDC80 homolog isoform X1
XM_006245657.3	XP_006245719.1	kinetochore protein NDC80 homolog isoform X1
NM_001126270.1	NP_001119742.1	kinetochore protein NDC80 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NDC80	XP_001146732.1
Bos taurus (cattle)	NDC80	NP_001193512.1
Mus musculus (house mouse)	Ndc80	NP_075783.2
Macaca mulatta (Rhesus monkey)	NDC80	XP_001086615.1
Gallus gallus (chicken)	NDC80	NP_989808.2
Danio rerio (zebrafish)	ndc80	NP_001003863.1
Oryza sativa (rice)	Os08g0468400	NP_001062008.1
Canis lupus familiaris (dog)	NDC80	XP_537313.2
Xenopus tropicalis (tropical clawed frog)	ndc80	NP_001011162.1
Arabidopsis thaliana (thale cress)	AT3G54630	NP_191024.1
Homo sapiens (human)	NDC80	NP_006092.1
Rattus norvegicus (Norway rat)	Ndc80	NP_001119742.1

Gene Ontology
Bibliography

Related articles in PubMed

Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules.
Iemura K, Tanaka K
Nature communications66447(2015 Mar)

Hec1 inhibition alters spindle morphology and chromosome alignment in porcine oocytes.
Wei X, Gao C, Luo J, Zhang W, Qi S, Liang W, Dai S
Molecular biology reports41(8)5089-95(2014 Aug)

Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
Shrestha RL, Draviam VM
Current biology : CB23(16)1514-26(2013 Aug)

Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment.
Varma D, Chandrasekaran S, Sundin LJ, Reidy KT, Wan X, Chasse DA, Nevis KR, DeLuca JG, Salmon ED, Cook JG
Nature cell biology14(6)593-603(2012 May)

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM
Science (New York, N.Y.)328(5978)593-9(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ndc80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndc80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01434	NM_001126270.1 Latest version!	Rattus norvegicus NDC80 kinetochore complex component (Ndc80), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa32666	XM_008767423.1 Latest version!	Rattus norvegicus NDC80 kinetochore complex component (Ndc80), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa32666	XM_006245657.3 Latest version!	Rattus norvegicus NDC80 kinetochore complex component (Ndc80), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndc80 ( NM_001126270.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001119742.1 Rattus norvegicus NDC80 kinetochore complex component (Ndc80), mRNA.

Price & Availability↑

CloneID	ORa01434
Clone ID Related Accession (Same CDS sequence)	NM_001126270.1
Accession Version	NM_001126270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Rattus norvegicus(Norway rat)
Product	kinetochore protein NDC80 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474043.1. On or before May 7, 2008 this sequence version replaced XM_001055564.1, XM_217489.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGCAATT TGGAGTCTCA TTCATCCATT CTGGAGCAGA AACTGAGCAG TCTTGATGAA 
GAGATTGGTA GACTGGAGCT AGAATGTGAG ACAGTTAAAC AGGAAAACAC TCGACTACAG 
AGTGTTGTTG ATAACCAGAA GTATTCGATC GCTGACATTG AGAGAATAAA TCATGAGAAA 
AATGAATTGC AGCAGACTAT AAATAAATTA ACCAAAGACC TGGAAGCCGA ACAACAACAA 
ATGTGGAATG AGGAATTAAA ATATGCCAGA GGCAAAGAGG CGATTGAAGC ACAGTTAGCA 
GAGTACCACA AGTTGGCTAG AAAACTGAAG CTTATCCCCA AGGGTGCTGA GAATTCCAAA 
GGTTATGACT TTGAAATTAA GTTTAATCCT GAGGCTGGTG CCAACTGCCT TGTCAAATAC 
AGGACTCAAG TGTATGCACC TCTCAAAGAA CTCTTGAATG AAATCGAAGA AGAAATTAAC 
AAAGCTCTGA ATAAAAAGAT GGGTCTGGAG GATACTTTAG AACAACTGAA CACCGTGAAA 
ACAGAAAGCA AGAGGGCTGT GAGAATGCTG AAGGAAGAGG TTCAAAAGCT GGACGACCTT 
TACCAACAAA AAGTTAAGGA AGCTGAGGAA GAAGACAAGA AGTGTGCCAG TGAGCTTGAC 
TCCCTGGAGA AACACAAGCA CCTGCTGGAA AGCGCAGTGA ACGAGGGTCT CAGCGAGGCC 
ATGGGCGAGC TGGACGCCGT CCAGCGGGAA TACCAGTTAG CTGTGCAGAC CACAACTGAA 
GAAAGAAGAA AGGTGGGAAA CAACTTACAA CGTCTTTTGG AGATGGTTGC TACACATGTA 
GGGTCTTTGG AGAAACACCT TGAAGAGCAG ATTGTTAAAG CTGACAGAGA GTATGAGGAA 
TGCACGTCTG AAGATCTCTT GGAAAACATC AGAAGGATCG CAGAGAAGTA TAAGAGCAAC 
GCTGCCCAAC TGAAGGCCCC CGACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119742.1
CDS	40..1029
Translation	MSNLESHSSILEQKLSSLDEEIGRLELECETVKQENTRLQSVVDNQKYSIADIERINHEKNELQQTINKLTKDLEAEQQQMWNEELKYARGKEAIEAQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRTQVYAPLKELLNEIEEEINKALNKKMGLEDTLEQLNTVKTESKRAVRMLKEEVQKLDDLYQQKVKEAEEEDKKCASELDSLEKHKHLLESAVNEGLSEAMGELDAVQREYQLAVQTTTEERRKVGNNLQRLLEMVATHVGSLEKHLEEQIVKADREYEECTSEDLLENIRRIAEKYKSNAAQLKAPDK

Target ORF information:

RefSeq Version	NM_001126270.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NDC80 kinetochore complex component (Ndc80), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126270.1

ORF Insert Sequence:

ATGAGCAATT TGGAGTCTCA TTCATCCATT CTGGAGCAGA AACTGAGCAG TCTTGATGAA 
GAGATTGGTA GACTGGAGCT AGAATGTGAG ACAGTTAAAC AGGAAAACAC TCGACTACAG 
AGTGTTGTTG ATAACCAGAA GTATTCGATC GCTGACATTG AGAGAATAAA TCATGAGAAA 
AATGAATTGC AGCAGACTAT AAATAAATTA ACCAAAGACC TGGAAGCCGA ACAACAACAA 
ATGTGGAATG AGGAATTAAA ATATGCCAGA GGCAAAGAGG CGATTGAAGC ACAGTTAGCA 
GAGTACCACA AGTTGGCTAG AAAACTGAAG CTTATCCCCA AGGGTGCTGA GAATTCCAAA 
GGTTATGACT TTGAAATTAA GTTTAATCCT GAGGCTGGTG CCAACTGCCT TGTCAAATAC 
AGGACTCAAG TGTATGCACC TCTCAAAGAA CTCTTGAATG AAATCGAAGA AGAAATTAAC 
AAAGCTCTGA ATAAAAAGAT GGGTCTGGAG GATACTTTAG AACAACTGAA CACCGTGAAA 
ACAGAAAGCA AGAGGGCTGT GAGAATGCTG AAGGAAGAGG TTCAAAAGCT GGACGACCTT 
TACCAACAAA AAGTTAAGGA AGCTGAGGAA GAAGACAAGA AGTGTGCCAG TGAGCTTGAC 
TCCCTGGAGA AACACAAGCA CCTGCTGGAA AGCGCAGTGA ACGAGGGTCT CAGCGAGGCC 
ATGGGCGAGC TGGACGCCGT CCAGCGGGAA TACCAGTTAG CTGTGCAGAC CACAACTGAA 
GAAAGAAGAA AGGTGGGAAA CAACTTACAA CGTCTTTTGG AGATGGTTGC TACACATGTA 
GGGTCTTTGG AGAAACACCT TGAAGAGCAG ATTGTTAAAG CTGACAGAGA GTATGAGGAA 
TGCACGTCTG AAGATCTCTT GGAAAACATC AGAAGGATCG CAGAGAAGTA TAAGAGCAAC 
GCTGCCCAAC TGAAGGCCCC CGACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndc80 ( XM_008767423.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765645.1 Rattus norvegicus NDC80 kinetochore complex component (Ndc80), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa32666
Clone ID Related Accession (Same CDS sequence)	XM_008767423.1 , XM_006245657.3
Accession Version	XM_008767423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	kinetochore protein NDC80 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCA GTTCGGTTTC CAGCTGTGGT GCTGGCCGCC TCTCTATGCA GGAGTTAAGA 
TCCCTGGACC TCAATAAACC TGGCCTTCAT ACCCCTCAAA CCAGAGAGAG GTCAACCTTT 
GGAAAACTGA GTACTCACAA GCCTACATCT GAAAGAAAAG TCTCAATATT TGGGAAAAGG 
ACTAGCGAAC ATGGGTCCAG GAATAGTCAA CTTGGTATAT TTTCCAGTTC TGAGAAAATC 
AAGGACCCAA GACCCCTTAA TGACAAAGCA TTCATTCAGC AGTGTATTCG ACAACTCTAT 
GAGTTTCTTA CAGAAAACGG TTATGTATAT AGTGTATCCA TGAAGTCTCT ACAAGCTCCA 
TCTACCAAAG ATTTCCTAAA GATCTTCGCC TTTCTTTATG GCTTTCTGTG CCCATCATAT 
GAACTTCCTG ATACAAAATG TGAAGAAGAG GTCCCAAGAA TTTTTAAAGA ACTTGGGTAT 
CCCTTTGCAC TGTCCAAGAG CTCCATGTAC ACAGTGGGAG CCCCTCACAC GTGGCCTCAC 
ATTGTGGCCG CCTTGGTGTG GCTAACAGAC TGCATCAAGA TTCATGATGC CATGAAACAA 
AGCTCACCTT TGTTTGATGA TGGGCAGGCT TGGGGAGAAG AAACTGAAGA TGGAATTAAG 
CATAATAAGT TGTTTTTGGA CTACACAAAA AAATGCTATG AGAAGTTCAT GACTGGAGCT 
GACACCTTCG AAGACGTGGA TGCTGAACTG CAGGCGAAGC TGAAGGACTT GTATAAGGTA 
GATGCGTCCA AGCTGGAATC ACTCGTGGAG GAAAACAAAG CACTGAATGA ACAGATTGCA 
AGACTGGAGC AGGAAAGAGA AAAAGAACCG AACCGTCTGA TATCATTGAG GAAACTGAAA 
GCTTCCTTAC AAGCAGATGT TCAAAACTAT AAAGCATACA TGAGCAATTT GGAGTCTCAT 
TCATCCATTC TGGAGCAGAA ACTGAGCAGT CTTGATGAAG AGATTGGTAG ACTGGAGCTA 
GAATGTGAGA CAGTTAAACA GGAAAACACT CGACTACAGA GTGTTGTTGA TAACCAGAAG 
TATTCGATCG CTGACATTGA GAGAATAAAT CATGAGAAAA ATGAATTGCA GCAGACTATA 
AATAAATTAA CCAAAGACCT GGAAGCCGAA CAACAACAAA TGTGGAATGA GGAATTAAAA 
TATGCCAGAG GCAAAGAGGC GATTGAAGCA CAGTTAGCAG AGTACCACAA GTTGGCTAGA 
AAACTGAAGC TTATCCCCAA GGGTGCTGAG AATTCCAAAG GTTATGACTT TGAAATTAAG 
TTTAATCCTG AGGCTGGTGC CAACTGCCTT GTCAAATACA GGACTCAAGT GTATGCACCT 
CTCAAAGAAC TCTTGAATGA AATCGAAGAA GAAATTAACA AAGCTCTGAA TAAAAAGATG 
GGTCTGGAGG ATACTTTAGA ACAACTGAAC ACCGTGAAAA CAGAAAGCAA GAGGGCTGTG 
AGAATGCTGA AGGAAGAGGT TCAAAAGCTG GACGACCTTT ACCAACAAAA AGTTAAGGAA 
GCTGAGGAAG AAGACAAGAA GTGTGCCAGT GAGCTTGACT CCCTGGAGAA ACACAAGCAC 
CTGCTGGAAA GCGCAGTGAA CGAGGGTCTC AGCGAGGCCA TGGGCGAGCT GGACGCCGTC 
CAGCGGGAAT ACCAGTTAGC TGTGCAGACC ACAACTGAAG AAAGAAGAAA GGTGGGAAAC 
AACTTACAAC GTCTTTTGGA GATGGTTGCT ACACATGTAG GGTCTTTGGA GAAACACCTT 
GAAGAGCAGA TTGTTAAAGC TGACAGAGAG TATGAGGAAT GCACGTCTGA AGATCTCTTG 
GAAAACATCA GAAGGATCGC AGAGAAGTAT AAGAGCAACG CTGCCCAACT GAAGGCCCCC 
GACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765645.1
CDS	151..2079
Translation	MKRSSVSSCGAGRLSMQELRSLDLNKPGLHTPQTRERSTFGKLSTHKPTSERKVSIFGKRTSEHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLYEFLTENGYVYSVSMKSLQAPSTKDFLKIFAFLYGFLCPSYELPDTKCEEEVPRIFKELGYPFALSKSSMYTVGAPHTWPHIVAALVWLTDCIKIHDAMKQSSPLFDDGQAWGEETEDGIKHNKLFLDYTKKCYEKFMTGADTFEDVDAELQAKLKDLYKVDASKLESLVEENKALNEQIARLEQEREKEPNRLISLRKLKASLQADVQNYKAYMSNLESHSSILEQKLSSLDEEIGRLELECETVKQENTRLQSVVDNQKYSIADIERINHEKNELQQTINKLTKDLEAEQQQMWNEELKYARGKEAIEAQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRTQVYAPLKELLNEIEEEINKALNKKMGLEDTLEQLNTVKTESKRAVRMLKEEVQKLDDLYQQKVKEAEEEDKKCASELDSLEKHKHLLESAVNEGLSEAMGELDAVQREYQLAVQTTTEERRKVGNNLQRLLEMVATHVGSLEKHLEEQIVKADREYEECTSEDLLENIRRIAEKYKSNAAQLKAPDK

Target ORF information:

RefSeq Version	XM_008767423.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NDC80 kinetochore complex component (Ndc80), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767423.1

ORF Insert Sequence:

ATGAAGCGCA GTTCGGTTTC CAGCTGTGGT GCTGGCCGCC TCTCTATGCA GGAGTTAAGA 
TCCCTGGACC TCAATAAACC TGGCCTTCAT ACCCCTCAAA CCAGAGAGAG GTCAACCTTT 
GGAAAACTGA GTACTCACAA GCCTACATCT GAAAGAAAAG TCTCAATATT TGGGAAAAGG 
ACTAGCGAAC ATGGGTCCAG GAATAGTCAA CTTGGTATAT TTTCCAGTTC TGAGAAAATC 
AAGGACCCAA GACCCCTTAA TGACAAAGCA TTCATTCAGC AGTGTATTCG ACAACTCTAT 
GAGTTTCTTA CAGAAAACGG TTATGTATAT AGTGTATCCA TGAAGTCTCT ACAAGCTCCA 
TCTACCAAAG ATTTCCTAAA GATCTTCGCC TTTCTTTATG GCTTTCTGTG CCCATCATAT 
GAACTTCCTG ATACAAAATG TGAAGAAGAG GTCCCAAGAA TTTTTAAAGA ACTTGGGTAT 
CCCTTTGCAC TGTCCAAGAG CTCCATGTAC ACAGTGGGAG CCCCTCACAC GTGGCCTCAC 
ATTGTGGCCG CCTTGGTGTG GCTAACAGAC TGCATCAAGA TTCATGATGC CATGAAACAA 
AGCTCACCTT TGTTTGATGA TGGGCAGGCT TGGGGAGAAG AAACTGAAGA TGGAATTAAG 
CATAATAAGT TGTTTTTGGA CTACACAAAA AAATGCTATG AGAAGTTCAT GACTGGAGCT 
GACACCTTCG AAGACGTGGA TGCTGAACTG CAGGCGAAGC TGAAGGACTT GTATAAGGTA 
GATGCGTCCA AGCTGGAATC ACTCGTGGAG GAAAACAAAG CACTGAATGA ACAGATTGCA 
AGACTGGAGC AGGAAAGAGA AAAAGAACCG AACCGTCTGA TATCATTGAG GAAACTGAAA 
GCTTCCTTAC AAGCAGATGT TCAAAACTAT AAAGCATACA TGAGCAATTT GGAGTCTCAT 
TCATCCATTC TGGAGCAGAA ACTGAGCAGT CTTGATGAAG AGATTGGTAG ACTGGAGCTA 
GAATGTGAGA CAGTTAAACA GGAAAACACT CGACTACAGA GTGTTGTTGA TAACCAGAAG 
TATTCGATCG CTGACATTGA GAGAATAAAT CATGAGAAAA ATGAATTGCA GCAGACTATA 
AATAAATTAA CCAAAGACCT GGAAGCCGAA CAACAACAAA TGTGGAATGA GGAATTAAAA 
TATGCCAGAG GCAAAGAGGC GATTGAAGCA CAGTTAGCAG AGTACCACAA GTTGGCTAGA 
AAACTGAAGC TTATCCCCAA GGGTGCTGAG AATTCCAAAG GTTATGACTT TGAAATTAAG 
TTTAATCCTG AGGCTGGTGC CAACTGCCTT GTCAAATACA GGACTCAAGT GTATGCACCT 
CTCAAAGAAC TCTTGAATGA AATCGAAGAA GAAATTAACA AAGCTCTGAA TAAAAAGATG 
GGTCTGGAGG ATACTTTAGA ACAACTGAAC ACCGTGAAAA CAGAAAGCAA GAGGGCTGTG 
AGAATGCTGA AGGAAGAGGT TCAAAAGCTG GACGACCTTT ACCAACAAAA AGTTAAGGAA 
GCTGAGGAAG AAGACAAGAA GTGTGCCAGT GAGCTTGACT CCCTGGAGAA ACACAAGCAC 
CTGCTGGAAA GCGCAGTGAA CGAGGGTCTC AGCGAGGCCA TGGGCGAGCT GGACGCCGTC 
CAGCGGGAAT ACCAGTTAGC TGTGCAGACC ACAACTGAAG AAAGAAGAAA GGTGGGAAAC 
AACTTACAAC GTCTTTTGGA GATGGTTGCT ACACATGTAG GGTCTTTGGA GAAACACCTT 
GAAGAGCAGA TTGTTAAAGC TGACAGAGAG TATGAGGAAT GCACGTCTGA AGATCTCTTG 
GAAAACATCA GAAGGATCGC AGAGAAGTAT AAGAGCAACG CTGCCCAACT GAAGGCCCCC 
GACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndc80 ( XM_006245657.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245719.1 Rattus norvegicus NDC80 kinetochore complex component (Ndc80), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32666
Clone ID Related Accession (Same CDS sequence)	XM_008767423.1 , XM_006245657.3
Accession Version	XM_006245657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	kinetochore protein NDC80 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245657.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCA GTTCGGTTTC CAGCTGTGGT GCTGGCCGCC TCTCTATGCA GGAGTTAAGA 
TCCCTGGACC TCAATAAACC TGGCCTTCAT ACCCCTCAAA CCAGAGAGAG GTCAACCTTT 
GGAAAACTGA GTACTCACAA GCCTACATCT GAAAGAAAAG TCTCAATATT TGGGAAAAGG 
ACTAGCGAAC ATGGGTCCAG GAATAGTCAA CTTGGTATAT TTTCCAGTTC TGAGAAAATC 
AAGGACCCAA GACCCCTTAA TGACAAAGCA TTCATTCAGC AGTGTATTCG ACAACTCTAT 
GAGTTTCTTA CAGAAAACGG TTATGTATAT AGTGTATCCA TGAAGTCTCT ACAAGCTCCA 
TCTACCAAAG ATTTCCTAAA GATCTTCGCC TTTCTTTATG GCTTTCTGTG CCCATCATAT 
GAACTTCCTG ATACAAAATG TGAAGAAGAG GTCCCAAGAA TTTTTAAAGA ACTTGGGTAT 
CCCTTTGCAC TGTCCAAGAG CTCCATGTAC ACAGTGGGAG CCCCTCACAC GTGGCCTCAC 
ATTGTGGCCG CCTTGGTGTG GCTAACAGAC TGCATCAAGA TTCATGATGC CATGAAACAA 
AGCTCACCTT TGTTTGATGA TGGGCAGGCT TGGGGAGAAG AAACTGAAGA TGGAATTAAG 
CATAATAAGT TGTTTTTGGA CTACACAAAA AAATGCTATG AGAAGTTCAT GACTGGAGCT 
GACACCTTCG AAGACGTGGA TGCTGAACTG CAGGCGAAGC TGAAGGACTT GTATAAGGTA 
GATGCGTCCA AGCTGGAATC ACTCGTGGAG GAAAACAAAG CACTGAATGA ACAGATTGCA 
AGACTGGAGC AGGAAAGAGA AAAAGAACCG AACCGTCTGA TATCATTGAG GAAACTGAAA 
GCTTCCTTAC AAGCAGATGT TCAAAACTAT AAAGCATACA TGAGCAATTT GGAGTCTCAT 
TCATCCATTC TGGAGCAGAA ACTGAGCAGT CTTGATGAAG AGATTGGTAG ACTGGAGCTA 
GAATGTGAGA CAGTTAAACA GGAAAACACT CGACTACAGA GTGTTGTTGA TAACCAGAAG 
TATTCGATCG CTGACATTGA GAGAATAAAT CATGAGAAAA ATGAATTGCA GCAGACTATA 
AATAAATTAA CCAAAGACCT GGAAGCCGAA CAACAACAAA TGTGGAATGA GGAATTAAAA 
TATGCCAGAG GCAAAGAGGC GATTGAAGCA CAGTTAGCAG AGTACCACAA GTTGGCTAGA 
AAACTGAAGC TTATCCCCAA GGGTGCTGAG AATTCCAAAG GTTATGACTT TGAAATTAAG 
TTTAATCCTG AGGCTGGTGC CAACTGCCTT GTCAAATACA GGACTCAAGT GTATGCACCT 
CTCAAAGAAC TCTTGAATGA AATCGAAGAA GAAATTAACA AAGCTCTGAA TAAAAAGATG 
GGTCTGGAGG ATACTTTAGA ACAACTGAAC ACCGTGAAAA CAGAAAGCAA GAGGGCTGTG 
AGAATGCTGA AGGAAGAGGT TCAAAAGCTG GACGACCTTT ACCAACAAAA AGTTAAGGAA 
GCTGAGGAAG AAGACAAGAA GTGTGCCAGT GAGCTTGACT CCCTGGAGAA ACACAAGCAC 
CTGCTGGAAA GCGCAGTGAA CGAGGGTCTC AGCGAGGCCA TGGGCGAGCT GGACGCCGTC 
CAGCGGGAAT ACCAGTTAGC TGTGCAGACC ACAACTGAAG AAAGAAGAAA GGTGGGAAAC 
AACTTACAAC GTCTTTTGGA GATGGTTGCT ACACATGTAG GGTCTTTGGA GAAACACCTT 
GAAGAGCAGA TTGTTAAAGC TGACAGAGAG TATGAGGAAT GCACGTCTGA AGATCTCTTG 
GAAAACATCA GAAGGATCGC AGAGAAGTAT AAGAGCAACG CTGCCCAACT GAAGGCCCCC 
GACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245719.1
CDS	138..2066
Translation	MKRSSVSSCGAGRLSMQELRSLDLNKPGLHTPQTRERSTFGKLSTHKPTSERKVSIFGKRTSEHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLYEFLTENGYVYSVSMKSLQAPSTKDFLKIFAFLYGFLCPSYELPDTKCEEEVPRIFKELGYPFALSKSSMYTVGAPHTWPHIVAALVWLTDCIKIHDAMKQSSPLFDDGQAWGEETEDGIKHNKLFLDYTKKCYEKFMTGADTFEDVDAELQAKLKDLYKVDASKLESLVEENKALNEQIARLEQEREKEPNRLISLRKLKASLQADVQNYKAYMSNLESHSSILEQKLSSLDEEIGRLELECETVKQENTRLQSVVDNQKYSIADIERINHEKNELQQTINKLTKDLEAEQQQMWNEELKYARGKEAIEAQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRTQVYAPLKELLNEIEEEINKALNKKMGLEDTLEQLNTVKTESKRAVRMLKEEVQKLDDLYQQKVKEAEEEDKKCASELDSLEKHKHLLESAVNEGLSEAMGELDAVQREYQLAVQTTTEERRKVGNNLQRLLEMVATHVGSLEKHLEEQIVKADREYEECTSEDLLENIRRIAEKYKSNAAQLKAPDK

Target ORF information:

RefSeq Version	XM_006245657.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NDC80 kinetochore complex component (Ndc80), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245657.3

ORF Insert Sequence:

ATGAAGCGCA GTTCGGTTTC CAGCTGTGGT GCTGGCCGCC TCTCTATGCA GGAGTTAAGA 
TCCCTGGACC TCAATAAACC TGGCCTTCAT ACCCCTCAAA CCAGAGAGAG GTCAACCTTT 
GGAAAACTGA GTACTCACAA GCCTACATCT GAAAGAAAAG TCTCAATATT TGGGAAAAGG 
ACTAGCGAAC ATGGGTCCAG GAATAGTCAA CTTGGTATAT TTTCCAGTTC TGAGAAAATC 
AAGGACCCAA GACCCCTTAA TGACAAAGCA TTCATTCAGC AGTGTATTCG ACAACTCTAT 
GAGTTTCTTA CAGAAAACGG TTATGTATAT AGTGTATCCA TGAAGTCTCT ACAAGCTCCA 
TCTACCAAAG ATTTCCTAAA GATCTTCGCC TTTCTTTATG GCTTTCTGTG CCCATCATAT 
GAACTTCCTG ATACAAAATG TGAAGAAGAG GTCCCAAGAA TTTTTAAAGA ACTTGGGTAT 
CCCTTTGCAC TGTCCAAGAG CTCCATGTAC ACAGTGGGAG CCCCTCACAC GTGGCCTCAC 
ATTGTGGCCG CCTTGGTGTG GCTAACAGAC TGCATCAAGA TTCATGATGC CATGAAACAA 
AGCTCACCTT TGTTTGATGA TGGGCAGGCT TGGGGAGAAG AAACTGAAGA TGGAATTAAG 
CATAATAAGT TGTTTTTGGA CTACACAAAA AAATGCTATG AGAAGTTCAT GACTGGAGCT 
GACACCTTCG AAGACGTGGA TGCTGAACTG CAGGCGAAGC TGAAGGACTT GTATAAGGTA 
GATGCGTCCA AGCTGGAATC ACTCGTGGAG GAAAACAAAG CACTGAATGA ACAGATTGCA 
AGACTGGAGC AGGAAAGAGA AAAAGAACCG AACCGTCTGA TATCATTGAG GAAACTGAAA 
GCTTCCTTAC AAGCAGATGT TCAAAACTAT AAAGCATACA TGAGCAATTT GGAGTCTCAT 
TCATCCATTC TGGAGCAGAA ACTGAGCAGT CTTGATGAAG AGATTGGTAG ACTGGAGCTA 
GAATGTGAGA CAGTTAAACA GGAAAACACT CGACTACAGA GTGTTGTTGA TAACCAGAAG 
TATTCGATCG CTGACATTGA GAGAATAAAT CATGAGAAAA ATGAATTGCA GCAGACTATA 
AATAAATTAA CCAAAGACCT GGAAGCCGAA CAACAACAAA TGTGGAATGA GGAATTAAAA 
TATGCCAGAG GCAAAGAGGC GATTGAAGCA CAGTTAGCAG AGTACCACAA GTTGGCTAGA 
AAACTGAAGC TTATCCCCAA GGGTGCTGAG AATTCCAAAG GTTATGACTT TGAAATTAAG 
TTTAATCCTG AGGCTGGTGC CAACTGCCTT GTCAAATACA GGACTCAAGT GTATGCACCT 
CTCAAAGAAC TCTTGAATGA AATCGAAGAA GAAATTAACA AAGCTCTGAA TAAAAAGATG 
GGTCTGGAGG ATACTTTAGA ACAACTGAAC ACCGTGAAAA CAGAAAGCAA GAGGGCTGTG 
AGAATGCTGA AGGAAGAGGT TCAAAAGCTG GACGACCTTT ACCAACAAAA AGTTAAGGAA 
GCTGAGGAAG AAGACAAGAA GTGTGCCAGT GAGCTTGACT CCCTGGAGAA ACACAAGCAC 
CTGCTGGAAA GCGCAGTGAA CGAGGGTCTC AGCGAGGCCA TGGGCGAGCT GGACGCCGTC 
CAGCGGGAAT ACCAGTTAGC TGTGCAGACC ACAACTGAAG AAAGAAGAAA GGTGGGAAAC 
AACTTACAAC GTCTTTTGGA GATGGTTGCT ACACATGTAG GGTCTTTGGA GAAACACCTT 
GAAGAGCAGA TTGTTAAAGC TGACAGAGAG TATGAGGAAT GCACGTCTGA AGATCTCTTG 
GAAAACATCA GAAGGATCGC AGAGAAGTAT AAGAGCAACG CTGCCCAACT GAAGGCCCCC 
GACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules.
Nature communications66447(2015 Mar)
Iemura K,Tanaka K

Hec1 inhibition alters spindle morphology and chromosome alignment in porcine oocytes.
Molecular biology reports41(8)5089-95(2014 Aug)
Wei X,Gao C,Luo J,Zhang W,Qi S,Liang W,Dai S

Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
Current biology : CB23(16)1514-26(2013 Aug)
Shrestha RL,Draviam VM

Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment.
Nature cell biology14(6)593-603(2012 May)
Varma D,Chandrasekaran S,Sundin LJ,Reidy KT,Wan X,Chasse DA,Nevis KR,DeLuca JG,Salmon ED,Cook JG

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Science (New York, N.Y.)328(5978)593-9(2010 Apr)
Hutchins JR,Toyoda Y,Hegemann B,Poser I,Hériché JK,Sykora MM,Augsburg M,Hudecz O,Buschhorn BA,Bulkescher J,Conrad C,Comartin D,Schleiffer A,Sarov M,Pozniakovsky A,Slabicki MM,Schloissnig S,Steinmacher I,Leuschner M,Ssykor A,Lawo S,Pelletier L,Stark H,Nasmyth K,Ellenberg J,Durbin R,Buchholz F,Mechtler K,Hyman AA,Peters JM

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Ndc80 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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