Uba7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Uba7
Entrez Gene ID	301000
Full Name	ubiquitin-like modifier activating enzyme 7
Synonyms	Ube1l
General protein information	Preferred Names ubiquitin-like modifier activating enzyme 7 Names ubiquitin-like modifier-activating enzyme 7
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243741.2	XP_006243803.1	ubiquitin-like modifier-activating enzyme 7 isoform X1
XM_006243742.2	XP_006243804.1	ubiquitin-like modifier-activating enzyme 7 isoform X2
XM_008766505.2	XP_008764727.1	ubiquitin-like modifier-activating enzyme 7 isoform X3
XM_006243743.3	XP_006243805.1	ubiquitin-like modifier-activating enzyme 7 isoform X4
NM_001106856.1	NP_001100326.1	ubiquitin-like modifier-activating enzyme 7

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBA7	NP_003326.2
Rattus norvegicus (Norway rat)	Uba7	NP_001100326.1
Canis lupus familiaris (dog)	UBA7	XP_850545.1
Mus musculus (house mouse)	Uba7	NP_076227.1
Pan troglodytes (chimpanzee)	UBA7	XP_001166289.1
Macaca mulatta (Rhesus monkey)	UBA7	XP_001105981.1
Bos taurus (cattle)	UBA7	NP_001012284.1

Gene Ontology
Bibliography

Related articles in PubMed

ISG15 Arg151 and the ISG15-conjugating enzyme UbE1L are important for innate immune control of Sindbis virus.
Giannakopoulos NV, Arutyunova E, Lai C, Lenschow DJ, Haas AL, Virgin HW
Journal of virology83(4)1602-10(2009 Feb)

The basis for selective E1-E2 interactions in the ISG15 conjugation system.
Durfee LA, Kelley ML, Huibregtse JM
The Journal of biological chemistry283(35)23895-902(2008 Aug)

Ube1L and protein ISGylation are not essential for alpha/beta interferon signaling.
Kim KI, Yan M, Malakhova O, Luo JK, Shen MF, Zou W, de la Torre JC, Zhang DE
Molecular and cellular biology26(2)472-9(2006 Jan)

Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme.
Takeuchi T, Iwahara S, Saeki Y, Sasajima H, Yokosawa H
Journal of biochemistry138(6)711-9(2005 Dec)

Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.
Giannakopoulos NV, Luo JK, Papov V, Zou W, Lenschow DJ, Jacobs BS, Borden EC, Li J, Virgin HW, Zhang DE
Biochemical and biophysical research communications336(2)496-506(2005 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Uba7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Uba7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05020	NM_001106856.1 Latest version!	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa31556	XM_006243741.2 Latest version!	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa31557	XM_006243742.2 Latest version!	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa31558	XM_006243743.3 Latest version!	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa45774	XM_008766505.2 Latest version!	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Uba7 ( NM_001106856.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100326.1 Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), mRNA.

Price & Availability↑

CloneID	ORa05020
Clone ID Related Accession (Same CDS sequence)	NM_001106856.1
Accession Version	NM_001106856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473954.2. On or before Oct 4, 2007 this sequence version replaced XM_001074704.1, XM_217252.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAAG AGCTGTACTC GAGGCAGCTG TATGTACTGG GCTTGCCTGC TATGCAGAGG 
ATCCGGGAAG CCAAAGTCTT GCTGTGTGGC CTGCAGGGCC TGGGAGCTGA GGTGGCCAAG 
AACCTGGTCC TTATGGGTGT GGGCAGCCTC ACCCTGCATG ATCCCCATCC CACCTGCTGG 
GCTGACTTGG CTGCTCAGTT TTTCCTCTCA GAAGAGAGCT TGGGAAGGAG CAGGGCTGAG 
GCCTCTCAAC CACAATTGGC CCAGCTTAAT GAAGCTGTCC AGATCTCCGT CCACAGAGGC 
GACATCACTG AGGACCTAGT GCGGGGTTTT CAGGTAGTGG TGCTGACTGA CTCCAAGCTA 
GAGGACCAGC TGAATATGGG AGCCTTGTGC CATAAGAATA GAGTCTACTT CCTCATGGCT 
GAAACCCGGG GCCTTGTGGG GCGGTTATTC TGTGATTTTG GTGAGGACTT CACTGTTGTG 
GACCCTACAG AGGTGGAGCC CACGTCAGCT GCCATCCAGG ACATCTCTCA GGGCTCCCCC 
GGCATTGTCA CTCTGAGAGG AGGCAGCAAA AGACCTTCCT TTTATGATGG GGACTTGGTG 
ATGTTCTCAG ACATTGAAGG CATGGTTGAA CTCAACAGTC ATTCTCCACA GCCTGTCCGT 
GTGCAGAAAG ATGGATCCTT GGAGATTGGA GATACAACAA CTTTCTCCCG TTACTTGAGG 
GGTGGGGTTG TCACTGAAGT CAAGAGACCC AAGACTGTGA AGCATAAGCC CCTGGACATA 
GCTCTGCTCC AGCCCTGCAT GGTGGTCCAG AATACTCAGG AAATACAACG CGCTCACTGC 
CTACACCAGA CCTTCCATGC ACTGCACAAG TTCCAGCAAC TTCATGGCCG GCTACCCAAG 
CCCTGGGATC CTGATGACGC AGAGACCGTG GTGTGGCTGG CCCAGGACCT AGAGCCACTA 
AAAGGGGCAA AAGAAGAGTC GCTGGATGAG GCTCTACTCC GGACAATTGC CCTGAGTAGT 
GCTGGTAGCT TAAGTCCCAT GGCAGCCATT CTGGGAGGAG TGGCTGCCCA GGAAGTCCTG 
AAGGCAGTCT CCGGGAAGTT CATGCCCCTG GACCAGTGGC TGTACTTTGA TGCCCTTGAA 
TGTCTTCCAG AAGATGAGAC GCTCCTGCCC AGTCCTGAGG ACTGTCATCC AAGAAACTGC 
CGATACGACG GGCAAATTGC TGTGTTCGGG ACTGGTTTTC AGGAGAAACT GAGCTACAAA 
CACTACCTCT TGGTAGGTGC TGGTGCCATT GGCTGTGAGA TGCTCAAAGG TTTTGCCCTA 
GTGGGCCTGG GAGTTAGGGA TAATGGCGGT GTGACAATTG CTGACATGGA CCACGTAGAA 
CGCTCCAACC TCAGCCGGCA GTTCCTCTTC AGGCCCAAGG ACACTGGGAG GCCCAAGGCA 
GAGGTGGCTG CAGAGGCAGC CCATCGCCTA AACCCAGACC TGCAAGTGAC TTCACATACC 
TGCCCTCTGG ATCCCACCAC AGAGGACATC TATGACGACG ACTTCTTCTC CAGGGTGGAC 
GGCGTTGTTG CTGCTTTGGA CAGTTTCCAG GCCCGGCACT ATGTTGCTGC TCGATGCACC 
CACTATTTGA AACCACTGCT GGAGGCAGGC ACCCAGGGAA CCCGGGGGAG TGCTTCAGTG 
TTTGTGCCAT ATGTGACAGA AGTCTACAAA GGTCCTACCT CAGCCGAGGA AGCTCCCTAC 
CCTGTGTGCA CCCTGCGGCA TTTTCCCAGC ACTGTGGAGC ACAGCCTGCA GTGGGCCCAG 
GACGAATTTG AGGGGCTCTT CAGACTATCC GCAGAGACCA TCAACGACTA CCAACAGTGC 
TTGAGGACGG AACTCTGTTC TGGTCAGGAT CCAAAAAGTG CCCACATCCC TTGCAATTTG 
ATCCCAACCA AGACACGCAT TTCCTCTATG TACTGGCAGC TGCCAACCTG TATGCACGGA 
TGCATGGGCT GCCGGGCTCA CAAAGTCAGA CTGCACTCAG GGAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100326.1
CDS	170..2200
Translation	MDEELYSRQLYVLGLPAMQRIREAKVLLCGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWADLAAQFFLSEESLGRSRAEASQPQLAQLNEAVQISVHRGDITEDLVRGFQVVVLTDSKLEDQLNMGALCHKNRVYFLMAETRGLVGRLFCDFGEDFTVVDPTEVEPTSAAIQDISQGSPGIVTLRGGSKRPSFYDGDLVMFSDIEGMVELNSHSPQPVRVQKDGSLEIGDTTTFSRYLRGGVVTEVKRPKTVKHKPLDIALLQPCMVVQNTQEIQRAHCLHQTFHALHKFQQLHGRLPKPWDPDDAETVVWLAQDLEPLKGAKEESLDEALLRTIALSSAGSLSPMAAILGGVAAQEVLKAVSGKFMPLDQWLYFDALECLPEDETLLPSPEDCHPRNCRYDGQIAVFGTGFQEKLSYKHYLLVGAGAIGCEMLKGFALVGLGVRDNGGVTIADMDHVERSNLSRQFLFRPKDTGRPKAEVAAEAAHRLNPDLQVTSHTCPLDPTTEDIYDDDFFSRVDGVVAALDSFQARHYVAARCTHYLKPLLEAGTQGTRGSASVFVPYVTEVYKGPTSAEEAPYPVCTLRHFPSTVEHSLQWAQDEFEGLFRLSAETINDYQQCLRTELCSGQDPKSAHIPCNLIPTKTRISSMYWQLPTCMHGCMGCRAHKVRLHSGNC

Target ORF information:

RefSeq Version	NM_001106856.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106856.1

ORF Insert Sequence:

ATGGATGAAG AGCTGTACTC GAGGCAGCTG TATGTACTGG GCTTGCCTGC TATGCAGAGG 
ATCCGGGAAG CCAAAGTCTT GCTGTGTGGC CTGCAGGGCC TGGGAGCTGA GGTGGCCAAG 
AACCTGGTCC TTATGGGTGT GGGCAGCCTC ACCCTGCATG ATCCCCATCC CACCTGCTGG 
GCTGACTTGG CTGCTCAGTT TTTCCTCTCA GAAGAGAGCT TGGGAAGGAG CAGGGCTGAG 
GCCTCTCAAC CACAATTGGC CCAGCTTAAT GAAGCTGTCC AGATCTCCGT CCACAGAGGC 
GACATCACTG AGGACCTAGT GCGGGGTTTT CAGGTAGTGG TGCTGACTGA CTCCAAGCTA 
GAGGACCAGC TGAATATGGG AGCCTTGTGC CATAAGAATA GAGTCTACTT CCTCATGGCT 
GAAACCCGGG GCCTTGTGGG GCGGTTATTC TGTGATTTTG GTGAGGACTT CACTGTTGTG 
GACCCTACAG AGGTGGAGCC CACGTCAGCT GCCATCCAGG ACATCTCTCA GGGCTCCCCC 
GGCATTGTCA CTCTGAGAGG AGGCAGCAAA AGACCTTCCT TTTATGATGG GGACTTGGTG 
ATGTTCTCAG ACATTGAAGG CATGGTTGAA CTCAACAGTC ATTCTCCACA GCCTGTCCGT 
GTGCAGAAAG ATGGATCCTT GGAGATTGGA GATACAACAA CTTTCTCCCG TTACTTGAGG 
GGTGGGGTTG TCACTGAAGT CAAGAGACCC AAGACTGTGA AGCATAAGCC CCTGGACATA 
GCTCTGCTCC AGCCCTGCAT GGTGGTCCAG AATACTCAGG AAATACAACG CGCTCACTGC 
CTACACCAGA CCTTCCATGC ACTGCACAAG TTCCAGCAAC TTCATGGCCG GCTACCCAAG 
CCCTGGGATC CTGATGACGC AGAGACCGTG GTGTGGCTGG CCCAGGACCT AGAGCCACTA 
AAAGGGGCAA AAGAAGAGTC GCTGGATGAG GCTCTACTCC GGACAATTGC CCTGAGTAGT 
GCTGGTAGCT TAAGTCCCAT GGCAGCCATT CTGGGAGGAG TGGCTGCCCA GGAAGTCCTG 
AAGGCAGTCT CCGGGAAGTT CATGCCCCTG GACCAGTGGC TGTACTTTGA TGCCCTTGAA 
TGTCTTCCAG AAGATGAGAC GCTCCTGCCC AGTCCTGAGG ACTGTCATCC AAGAAACTGC 
CGATACGACG GGCAAATTGC TGTGTTCGGG ACTGGTTTTC AGGAGAAACT GAGCTACAAA 
CACTACCTCT TGGTAGGTGC TGGTGCCATT GGCTGTGAGA TGCTCAAAGG TTTTGCCCTA 
GTGGGCCTGG GAGTTAGGGA TAATGGCGGT GTGACAATTG CTGACATGGA CCACGTAGAA 
CGCTCCAACC TCAGCCGGCA GTTCCTCTTC AGGCCCAAGG ACACTGGGAG GCCCAAGGCA 
GAGGTGGCTG CAGAGGCAGC CCATCGCCTA AACCCAGACC TGCAAGTGAC TTCACATACC 
TGCCCTCTGG ATCCCACCAC AGAGGACATC TATGACGACG ACTTCTTCTC CAGGGTGGAC 
GGCGTTGTTG CTGCTTTGGA CAGTTTCCAG GCCCGGCACT ATGTTGCTGC TCGATGCACC 
CACTATTTGA AACCACTGCT GGAGGCAGGC ACCCAGGGAA CCCGGGGGAG TGCTTCAGTG 
TTTGTGCCAT ATGTGACAGA AGTCTACAAA GGTCCTACCT CAGCCGAGGA AGCTCCCTAC 
CCTGTGTGCA CCCTGCGGCA TTTTCCCAGC ACTGTGGAGC ACAGCCTGCA GTGGGCCCAG 
GACGAATTTG AGGGGCTCTT CAGACTATCC GCAGAGACCA TCAACGACTA CCAACAGTGC 
TTGAGGACGG AACTCTGTTC TGGTCAGGAT CCAAAAAGTG CCCACATCCC TTGCAATTTG 
ATCCCAACCA AGACACGCAT TTCCTCTATG TACTGGCAGC TGCCAACCTG TATGCACGGA 
TGCATGGGCT GCCGGGCTCA CAAAGTCAGA CTGCACTCAG GGAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba7 ( XM_006243741.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243803.1 Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31556
Clone ID Related Accession (Same CDS sequence)	XM_006243741.2
Accession Version	XM_006243741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006243741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCTGTACTC GAGGCAGCTG TATGTACTGG GCTTGCCTGC TATGCAGAGG 
ATCCGGGAAG CCAAAGTCTT GCTGTGTGGC CTGCAGGGCC TGGGAGCTGA GGTGGCCAAG 
AACCTGGTCC TTATGGGTGT GGGCAGCCTC ACCCTGCATG ATCCCCATCC CACCTGCTGG 
GCTGACTTGG CTGCTCAGTT TTTCCTCTCA GAAGAGAGCT TGGGAAGGAG CAGGGCTGAG 
GCCTCTCAAC CACAATTGGC CCAGCTTAAT GAAGCTGTCC AGATCTCCGT CCACAGAGGC 
GACATCACTG AGGACCTAGT GCGGGGTTTT CAGGTAGTGG TGCTGACTGA CTCCAAGCTA 
GAGGACCAGC TGAATATGGG AGCCTTGTGC CATAAGAATA GAGTCTACTT CCTCATGGCT 
GAAACCCGGG GCCTTGTGGG GCGGTTATTC TGTGATTTTG GTGAGGACTT CACTGTTGTG 
GACCCTACAG AGGTGGAGCC CACGTCAGCT GCCATCCAGG ACATCTCTCA GGGCTCCCCC 
GGCATTGTCA CTCTGAGAGG AGGCAGCAAA AGACCTTCCT TTTATGATGG GGACTTGGTG 
ATGTTCTCAG ACATTGAAGG CATGGTTGAA CTCAACAGTC ATTCTCCACA GCCTGTCCGT 
GTGCAGAAAG ATGGATCCTT GGAGATTGGA GATACAACAA CTTTCTCCCG TTACTTGAGG 
GGTGGGGTTG TCACTGAAGT CAAGAGACCC AAGACTGTGA AGCATAAGCC CCTGGACATA 
GCTCTGCTCC AGCCCTGCAT GGTGGTCCAG AATACTCAGG AAATACAACG CGCTCACTGC 
CTACACCAGA CCTTCCATGC ACTGCACAAG TTCCAGCAAC TTCATGGCCG GCTACCCAAG 
CCCTGGGATC CTGATGACGC AGAGACCGTG GTGTGGCTGG CCCAGGACCT AGAGCCACTA 
AAAGGGGCAA AAGAAGAGTC GCTGGATGAG GCTCTACTCC GGACAATTGC CCTGAGTAGT 
GCTGGTAGCT TAAGTCCCAT GGCAGCCATT CTGGGAGGAG TGGCTGCCCA GGAAGTCCTG 
AAGGCAGTCT CCGGGAAGTT CATGCCCCTG GACCAGTGGC TGTACTTTGA TGCCCTTGAA 
TGTCTTCCAG AAGATGAGAC GCTCCTGCCC AGTCCTGAGG ACTGTCATCC AAGAAACTGC 
CGATACGACG GGCAAATTGC TGTGTTCGGG ACTGGTTTTC AGGAGAAACT GAGCTACAAA 
CACTACCTCT TGGTAGGTGC TGGTGCCATT GGCTGTGAGA TGCTCAAAGG TTTTGCCCTA 
GTGGGCCTGG GAGTTAGGGA TAATGGCGGT GTGACAATTG CTGACATGGA CCACGTAGAA 
CGCTCCAACC TCAGCCGGCA GTTCCTCTTC AGGCCCAAGG ACACTGGGAG GCCCAAGGCA 
GAGGTGGCTG CAGAGGCAGC CCATCGCCTA AACCCAGACC TGCAAGTGAC TTCACATACC 
TGCCCTCTGG ATCCCACCAC AGAGGACATC TATGACGACG ACTTCTTCTC CAGGGTGGAC 
GGCGTTGTTG CTGCTTTGGA CAGTTTCCAG GCCCGGCACT ATGTTGCTGC TCGATGCACC 
CACTATTTGA AACCACTGCT GGAGGCAGGC ACCCAGGGAA CCCGGGGGAG TGCTTCAGTG 
TTTGTGCCAT ATGTGACAGA AGTCTACAAA GGTCCTACCT CAGCCGAGGA AGCTCCCTAC 
CCTGTGTGCA CCCTGCGGCA TTTTCCCAGC ACTGTGGAGC ACAGCCTGCA GTGGGCCCAG 
GACGAATTTG AGGGGCTCTT CAGACTATCC GCAGAGACCA TCAACGACTA CCAACAGACA 
TGCACTTCCT CCCTGTTGGG CACCGATGGG ACTGGAATAT TGGCCTTACT GCAGCAAGTG 
ATGGGCGTCC TAAGAACTCG GCCACAGACC TGGCAAGACT GTGTGGTGTG GGCTCTTGGC 
CACTGGCAAC TGTGCTTCCG TGATAACATC GTGGAGCTGC TGAGAAAATT CCCATCTGAT 
AAAGTGCTTG AGGACGGAAC TCTGTTCTGG TCAGGATCCA AAAAGTGCCC ACATCCCTTG 
CAATTTGATC CCAACCAAGA CACGCATTTC CTCTATGTAC TGGCAGCTGC CAACCTGTAT 
GCACGGATGC ATGGGCTGCC GGGCTCACAA AGTCAGACTG CACTCAGGGA ACTGCTGACA 
CTGACACTGC TGCAGGAGCC TGATTCCATG CCCCAGAACC TCTTCTCTGC TGAGTTTGGC 
CAGGAACAGT TGAAGGAACT GCAGGAAACC CTGGATGACT GGAACAAGGG GCCTCCACTG 
AAGCCTGTGC TGTTTGGGAA GGATGACAGC AGCAACTTCC ACGAGGACTT TGTAGTAGCA 
GCGACAGACC TGCGCTGTCA GAACTATGGG ATCCTGCCAG TTAACCGTGC TCGGATCAAG 
CAAACCATCG GCCGGATTAT CCCAGCCATT GCCACCAGTA CAGCAGTTGT GGCGGGCTTA 
CTGGGCCTGG AGCTGTATAA GGTGGTAAGT GGGCCACGGC CCCTTGGCAC CTTTCGGCAC 
AGCTACCTGC ACCTGGCTGA AAACCACTTC ATACGCTCAG CACCTTCTGC CCCGGCCATG 
CAGTCGTTCC ATCACCTGGA ATGGACCTGT TGGCACCGCC TGACGGTGCC TGCTGGGCAG 
CCTGAGAGGA CACTGGAATC CCTGCTGGCC CATCTCCAGG AAGAGCATGG ACTGACGGTG 
AGGATGCTGC TGCATGACCA GGCTCTGCTC TATTCTTCAG GATGGCCATT TGAAAAGCAG 
GCTCAGCACT TGGGCCTCAG GGTGACAGAA CTAGTTCAGC AGGTGACCGG CCAGAAACCC 
AAGCCTGGGC TGAGAGTTCT GGTGTTTGAG CTGAGCTGTG AGGGTGAGGA TGACGAAATG 
GCCTTCCCAC CTCTGCATTA TGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243803.1
CDS	122..3091
Translation	MDEELYSRQLYVLGLPAMQRIREAKVLLCGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWADLAAQFFLSEESLGRSRAEASQPQLAQLNEAVQISVHRGDITEDLVRGFQVVVLTDSKLEDQLNMGALCHKNRVYFLMAETRGLVGRLFCDFGEDFTVVDPTEVEPTSAAIQDISQGSPGIVTLRGGSKRPSFYDGDLVMFSDIEGMVELNSHSPQPVRVQKDGSLEIGDTTTFSRYLRGGVVTEVKRPKTVKHKPLDIALLQPCMVVQNTQEIQRAHCLHQTFHALHKFQQLHGRLPKPWDPDDAETVVWLAQDLEPLKGAKEESLDEALLRTIALSSAGSLSPMAAILGGVAAQEVLKAVSGKFMPLDQWLYFDALECLPEDETLLPSPEDCHPRNCRYDGQIAVFGTGFQEKLSYKHYLLVGAGAIGCEMLKGFALVGLGVRDNGGVTIADMDHVERSNLSRQFLFRPKDTGRPKAEVAAEAAHRLNPDLQVTSHTCPLDPTTEDIYDDDFFSRVDGVVAALDSFQARHYVAARCTHYLKPLLEAGTQGTRGSASVFVPYVTEVYKGPTSAEEAPYPVCTLRHFPSTVEHSLQWAQDEFEGLFRLSAETINDYQQTCTSSLLGTDGTGILALLQQVMGVLRTRPQTWQDCVVWALGHWQLCFRDNIVELLRKFPSDKVLEDGTLFWSGSKKCPHPLQFDPNQDTHFLYVLAAANLYARMHGLPGSQSQTALRELLTLTLLQEPDSMPQNLFSAEFGQEQLKELQETLDDWNKGPPLKPVLFGKDDSSNFHEDFVVAATDLRCQNYGILPVNRARIKQTIGRIIPAIATSTAVVAGLLGLELYKVVSGPRPLGTFRHSYLHLAENHFIRSAPSAPAMQSFHHLEWTCWHRLTVPAGQPERTLESLLAHLQEEHGLTVRMLLHDQALLYSSGWPFEKQAQHLGLRVTELVQQVTGQKPKPGLRVLVFELSCEGEDDEMAFPPLHYEL

Target ORF information:

RefSeq Version	XM_006243741.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243741.2

ORF Insert Sequence:

ATGGATGAAG AGCTGTACTC GAGGCAGCTG TATGTACTGG GCTTGCCTGC TATGCAGAGG 
ATCCGGGAAG CCAAAGTCTT GCTGTGTGGC CTGCAGGGCC TGGGAGCTGA GGTGGCCAAG 
AACCTGGTCC TTATGGGTGT GGGCAGCCTC ACCCTGCATG ATCCCCATCC CACCTGCTGG 
GCTGACTTGG CTGCTCAGTT TTTCCTCTCA GAAGAGAGCT TGGGAAGGAG CAGGGCTGAG 
GCCTCTCAAC CACAATTGGC CCAGCTTAAT GAAGCTGTCC AGATCTCCGT CCACAGAGGC 
GACATCACTG AGGACCTAGT GCGGGGTTTT CAGGTAGTGG TGCTGACTGA CTCCAAGCTA 
GAGGACCAGC TGAATATGGG AGCCTTGTGC CATAAGAATA GAGTCTACTT CCTCATGGCT 
GAAACCCGGG GCCTTGTGGG GCGGTTATTC TGTGATTTTG GTGAGGACTT CACTGTTGTG 
GACCCTACAG AGGTGGAGCC CACGTCAGCT GCCATCCAGG ACATCTCTCA GGGCTCCCCC 
GGCATTGTCA CTCTGAGAGG AGGCAGCAAA AGACCTTCCT TTTATGATGG GGACTTGGTG 
ATGTTCTCAG ACATTGAAGG CATGGTTGAA CTCAACAGTC ATTCTCCACA GCCTGTCCGT 
GTGCAGAAAG ATGGATCCTT GGAGATTGGA GATACAACAA CTTTCTCCCG TTACTTGAGG 
GGTGGGGTTG TCACTGAAGT CAAGAGACCC AAGACTGTGA AGCATAAGCC CCTGGACATA 
GCTCTGCTCC AGCCCTGCAT GGTGGTCCAG AATACTCAGG AAATACAACG CGCTCACTGC 
CTACACCAGA CCTTCCATGC ACTGCACAAG TTCCAGCAAC TTCATGGCCG GCTACCCAAG 
CCCTGGGATC CTGATGACGC AGAGACCGTG GTGTGGCTGG CCCAGGACCT AGAGCCACTA 
AAAGGGGCAA AAGAAGAGTC GCTGGATGAG GCTCTACTCC GGACAATTGC CCTGAGTAGT 
GCTGGTAGCT TAAGTCCCAT GGCAGCCATT CTGGGAGGAG TGGCTGCCCA GGAAGTCCTG 
AAGGCAGTCT CCGGGAAGTT CATGCCCCTG GACCAGTGGC TGTACTTTGA TGCCCTTGAA 
TGTCTTCCAG AAGATGAGAC GCTCCTGCCC AGTCCTGAGG ACTGTCATCC AAGAAACTGC 
CGATACGACG GGCAAATTGC TGTGTTCGGG ACTGGTTTTC AGGAGAAACT GAGCTACAAA 
CACTACCTCT TGGTAGGTGC TGGTGCCATT GGCTGTGAGA TGCTCAAAGG TTTTGCCCTA 
GTGGGCCTGG GAGTTAGGGA TAATGGCGGT GTGACAATTG CTGACATGGA CCACGTAGAA 
CGCTCCAACC TCAGCCGGCA GTTCCTCTTC AGGCCCAAGG ACACTGGGAG GCCCAAGGCA 
GAGGTGGCTG CAGAGGCAGC CCATCGCCTA AACCCAGACC TGCAAGTGAC TTCACATACC 
TGCCCTCTGG ATCCCACCAC AGAGGACATC TATGACGACG ACTTCTTCTC CAGGGTGGAC 
GGCGTTGTTG CTGCTTTGGA CAGTTTCCAG GCCCGGCACT ATGTTGCTGC TCGATGCACC 
CACTATTTGA AACCACTGCT GGAGGCAGGC ACCCAGGGAA CCCGGGGGAG TGCTTCAGTG 
TTTGTGCCAT ATGTGACAGA AGTCTACAAA GGTCCTACCT CAGCCGAGGA AGCTCCCTAC 
CCTGTGTGCA CCCTGCGGCA TTTTCCCAGC ACTGTGGAGC ACAGCCTGCA GTGGGCCCAG 
GACGAATTTG AGGGGCTCTT CAGACTATCC GCAGAGACCA TCAACGACTA CCAACAGACA 
TGCACTTCCT CCCTGTTGGG CACCGATGGG ACTGGAATAT TGGCCTTACT GCAGCAAGTG 
ATGGGCGTCC TAAGAACTCG GCCACAGACC TGGCAAGACT GTGTGGTGTG GGCTCTTGGC 
CACTGGCAAC TGTGCTTCCG TGATAACATC GTGGAGCTGC TGAGAAAATT CCCATCTGAT 
AAAGTGCTTG AGGACGGAAC TCTGTTCTGG TCAGGATCCA AAAAGTGCCC ACATCCCTTG 
CAATTTGATC CCAACCAAGA CACGCATTTC CTCTATGTAC TGGCAGCTGC CAACCTGTAT 
GCACGGATGC ATGGGCTGCC GGGCTCACAA AGTCAGACTG CACTCAGGGA ACTGCTGACA 
CTGACACTGC TGCAGGAGCC TGATTCCATG CCCCAGAACC TCTTCTCTGC TGAGTTTGGC 
CAGGAACAGT TGAAGGAACT GCAGGAAACC CTGGATGACT GGAACAAGGG GCCTCCACTG 
AAGCCTGTGC TGTTTGGGAA GGATGACAGC AGCAACTTCC ACGAGGACTT TGTAGTAGCA 
GCGACAGACC TGCGCTGTCA GAACTATGGG ATCCTGCCAG TTAACCGTGC TCGGATCAAG 
CAAACCATCG GCCGGATTAT CCCAGCCATT GCCACCAGTA CAGCAGTTGT GGCGGGCTTA 
CTGGGCCTGG AGCTGTATAA GGTGGTAAGT GGGCCACGGC CCCTTGGCAC CTTTCGGCAC 
AGCTACCTGC ACCTGGCTGA AAACCACTTC ATACGCTCAG CACCTTCTGC CCCGGCCATG 
CAGTCGTTCC ATCACCTGGA ATGGACCTGT TGGCACCGCC TGACGGTGCC TGCTGGGCAG 
CCTGAGAGGA CACTGGAATC CCTGCTGGCC CATCTCCAGG AAGAGCATGG ACTGACGGTG 
AGGATGCTGC TGCATGACCA GGCTCTGCTC TATTCTTCAG GATGGCCATT TGAAAAGCAG 
GCTCAGCACT TGGGCCTCAG GGTGACAGAA CTAGTTCAGC AGGTGACCGG CCAGAAACCC 
AAGCCTGGGC TGAGAGTTCT GGTGTTTGAG CTGAGCTGTG AGGGTGAGGA TGACGAAATG 
GCCTTCCCAC CTCTGCATTA TGAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba7 ( XM_006243742.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243804.1 Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa31557
Clone ID Related Accession (Same CDS sequence)	XM_006243742.2
Accession Version	XM_006243742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006243742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCTGTACTC GAGGCAGCTG TATGTACTGG GCTTGCCTGC TATGCAGAGG 
ATCCGGGAAG CCAAAGTCTT GCTGTGTGGC CTGCAGGGCC TGGGAGCTGA GGTGGCCAAG 
AACCTGGTCC TTATGGGTGT GGGCAGCCTC ACCCTGCATG ATCCCCATCC CACCTGCTGG 
GCTGACTTGG CTGCTCAGTT TTTCCTCTCA GAAGAGAGCT TGGGAAGGAG CAGGGCTGAG 
GCCTCTCAAC CACAATTGGC CCAGCTTAAT GAAGCTGTCC AGATCTCCGT CCACAGAGGC 
GACATCACTG AGGACCTAGT GCGGGGTTTT CAGGTAGTGG TGCTGACTGA CTCCAAGCTA 
GAGGACCAGC TGAATATGGG AGCCTTGTGC CATAAGAATA GAGTCTACTT CCTCATGGCT 
GAAACCCGGG GCCTTGTGGG GCGGTTATTC TGTGATTTTG GTGAGGACTT CACTGTTGTG 
GACCCTACAG AGGTGGAGCC CACGTCAGCT GCCATCCAGG ACATCTCTCA GGGCTCCCCC 
GGCATTGTCA CTCTGAGAGG AGGCAGCAAA AGACCTTCCT TTTATGATGG GGACTTGGTG 
ATGTTCTCAG ACATTGAAGG CATGGTTGAA CTCAACAGTC ATTCTCCACA GCCTGTCCGT 
GTGCAGAAAG ATGGATCCTT GGAGATTGGA GATACAACAA CTTTCTCCCG TTACTTGAGG 
GGTGGGGTTG TCACTGAAGT CAAGAGACCC AAGACTGTGA AGCATAAGCC CCTGGACATA 
GCTCTGCTCC AGCCCTGCAT GGTGGTCCAG AATACTCAGG AAATACAACG CGCTCACTGC 
CTACACCAGA CCTTCCATGC ACTGCACAAG TTCCAGCAAC TTCATGGCCG GCTACCCAAG 
CCCTGGGATC CTGATGACGC AGAGACCGTG GTGTGGCTGG CCCAGGACCT AGAGCCACTA 
AAAGGGGCAA AAGAAGAGTC GCTGGATGAG GCTCTACTCC GGACAATTGC CCTGAGTAGT 
GCTGGTAGCT TAAGTCCCAT GGCAGCCATT CTGGGAGGAG TGGCTGCCCA GGAAGTCCTG 
AAGGCAGTCT CCGGGAAGTT CATGCCCCTG GACCAGTGGC TGTACTTTGA TGCCCTTGAA 
TGTCTTCCAG AAGATGAGAC GCTCCTGCCC AGTCCTGAGG ACTGTCATCC AAGAAACTGC 
CGATACGACG GGCAAATTGC TGTGTTCGGG ACTGGTTTTC AGGAGAAACT GAGCTACAAA 
CACTACCTCT TGGTAGGTGC TGGTGCCATT GGCTGTGAGA TGCTCAAAGG TTTTGCCCTA 
GTGGGCCTGG GAGTTAGGGA TAATGGCGGT GTGACAATTG CTGACATGGA CCACGTAGAA 
CGCTCCAACC TCAGCCGGCA GTTCCTCTTC AGGCCCAAGG ACACTGGGAG GCCCAAGGCA 
GAGGTGGCTG CAGAGGCAGC CCATCGCCTA AACCCAGACC TGCAAGTGAC TTCACATACC 
TGCCCTCTGG ATCCCACCAC AGAGGACATC TATGACGACG ACTTCTTCTC CAGGGTGGAC 
GGCGTTGTTG CTGCTTTGGA CAGTTTCCAG GCCCGGCACT ATGTTGCTGC TCGATGCACC 
CACTATTTGA AACCACTGCT GGAGGCAGGC ACCCAGGGAA CCCGGGGGAG TGCTTCAGTG 
TTTGTGCCAT ATGTGACAGA AGTCTACAAA GGTCCTACCT CAGCCGAGGA AGCTCCCTAC 
CCTGTGTGCA CCCTGCGGCA TTTTCCCAGC ACTGTGGAGC ACAGCCTGCA GTGGGCCCAG 
GACGAATTTG AGGGGCTCTT CAGACTATCC GCAGAGACCA TCAACGACTA CCAACAGACA 
TGCACTTCCT CCCTGTTGGG CACCGATGGG ACTGGAATAT TGGCCTTACT GCAGCAAGTG 
ATGGGCGTCC TAAGAACTCG GCCACAGACC TGGCAAGACT GTGTGGTGTG GGCTCTTGGC 
CACTGGCAAC TGTGCTTCCG TGATAACATC GTGGAGCTGC TGAGAAAATT CCCATCTGAT 
AAAGTGCTTG AGGACGGAAC TCTGTTCTGG TCAGGATCCA AAAAGTGCCC ACATCCCTTG 
CAATTTGATC CCAACCAAGA CACGCATTTC CTCTATGTAC TGGCAGCTGC CAACCTGTAT 
GCACGGATGC ATGGGCTGCC GGGCTCACAA AGTCAGACTG CACTCAGGGA ACTGCTGACA 
CTGACACTGC TGCAGGAGCC TGATTCCATG CCCCAGAACC TCTTCTCTGC TGAGTTTGGC 
CAGGAACAGT TGAAGGAACT GCAGGAAACC CTGGATGACT GGAACAAGGG GCCTCCACTG 
AAGCCTGTGC TGTTTGGGAA GGATGACAGC AGCAACTTCC ACGAGGACTT TGTAGTAGCA 
GCGACAGACC TGCGCTGTCA GAACTATGGG ATCCTGCCAG TTAACCGTGC TCGGATCAAG 
CAAACCATCG GCCGGATTAT CCCAGCCATT GCCACCAGTA CAGCAGTTGT GGCGGGCTTA 
CTGGGCCTGG AGCTGTATAA GGTGGTAAGT GGGCCACGGC CCCTTGGCAC CTTTCGGCAC 
AGCTACCTGC ACCTGGCTGA AAACCACTTC ATACGCTCAG CACCTTCTGC CCCGGCCATG 
CAGTCGTTCC ATCACCTGGA ATGGACCTGT TGGCACCGCC TGACGGTGCC TGCTGGGCAG 
CCTGAGAGGA CACTGGAATC CCTGCTGGCC CATCTCCAGG AAGAGCATGG ACTGACGGTG 
AGGATGCTGC TGCATGACCA GGCTCTGCTC TATTCTTCAG GATGGCCATT TGAAAAGCAG 
GCTCAGCACT TGGGCCTCAG ATCTTGCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243804.1
CDS	122..2974
Translation	MDEELYSRQLYVLGLPAMQRIREAKVLLCGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWADLAAQFFLSEESLGRSRAEASQPQLAQLNEAVQISVHRGDITEDLVRGFQVVVLTDSKLEDQLNMGALCHKNRVYFLMAETRGLVGRLFCDFGEDFTVVDPTEVEPTSAAIQDISQGSPGIVTLRGGSKRPSFYDGDLVMFSDIEGMVELNSHSPQPVRVQKDGSLEIGDTTTFSRYLRGGVVTEVKRPKTVKHKPLDIALLQPCMVVQNTQEIQRAHCLHQTFHALHKFQQLHGRLPKPWDPDDAETVVWLAQDLEPLKGAKEESLDEALLRTIALSSAGSLSPMAAILGGVAAQEVLKAVSGKFMPLDQWLYFDALECLPEDETLLPSPEDCHPRNCRYDGQIAVFGTGFQEKLSYKHYLLVGAGAIGCEMLKGFALVGLGVRDNGGVTIADMDHVERSNLSRQFLFRPKDTGRPKAEVAAEAAHRLNPDLQVTSHTCPLDPTTEDIYDDDFFSRVDGVVAALDSFQARHYVAARCTHYLKPLLEAGTQGTRGSASVFVPYVTEVYKGPTSAEEAPYPVCTLRHFPSTVEHSLQWAQDEFEGLFRLSAETINDYQQTCTSSLLGTDGTGILALLQQVMGVLRTRPQTWQDCVVWALGHWQLCFRDNIVELLRKFPSDKVLEDGTLFWSGSKKCPHPLQFDPNQDTHFLYVLAAANLYARMHGLPGSQSQTALRELLTLTLLQEPDSMPQNLFSAEFGQEQLKELQETLDDWNKGPPLKPVLFGKDDSSNFHEDFVVAATDLRCQNYGILPVNRARIKQTIGRIIPAIATSTAVVAGLLGLELYKVVSGPRPLGTFRHSYLHLAENHFIRSAPSAPAMQSFHHLEWTCWHRLTVPAGQPERTLESLLAHLQEEHGLTVRMLLHDQALLYSSGWPFEKQAQHLGLRSCC

Target ORF information:

RefSeq Version	XM_006243742.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243742.2

ORF Insert Sequence:

ATGGATGAAG AGCTGTACTC GAGGCAGCTG TATGTACTGG GCTTGCCTGC TATGCAGAGG 
ATCCGGGAAG CCAAAGTCTT GCTGTGTGGC CTGCAGGGCC TGGGAGCTGA GGTGGCCAAG 
AACCTGGTCC TTATGGGTGT GGGCAGCCTC ACCCTGCATG ATCCCCATCC CACCTGCTGG 
GCTGACTTGG CTGCTCAGTT TTTCCTCTCA GAAGAGAGCT TGGGAAGGAG CAGGGCTGAG 
GCCTCTCAAC CACAATTGGC CCAGCTTAAT GAAGCTGTCC AGATCTCCGT CCACAGAGGC 
GACATCACTG AGGACCTAGT GCGGGGTTTT CAGGTAGTGG TGCTGACTGA CTCCAAGCTA 
GAGGACCAGC TGAATATGGG AGCCTTGTGC CATAAGAATA GAGTCTACTT CCTCATGGCT 
GAAACCCGGG GCCTTGTGGG GCGGTTATTC TGTGATTTTG GTGAGGACTT CACTGTTGTG 
GACCCTACAG AGGTGGAGCC CACGTCAGCT GCCATCCAGG ACATCTCTCA GGGCTCCCCC 
GGCATTGTCA CTCTGAGAGG AGGCAGCAAA AGACCTTCCT TTTATGATGG GGACTTGGTG 
ATGTTCTCAG ACATTGAAGG CATGGTTGAA CTCAACAGTC ATTCTCCACA GCCTGTCCGT 
GTGCAGAAAG ATGGATCCTT GGAGATTGGA GATACAACAA CTTTCTCCCG TTACTTGAGG 
GGTGGGGTTG TCACTGAAGT CAAGAGACCC AAGACTGTGA AGCATAAGCC CCTGGACATA 
GCTCTGCTCC AGCCCTGCAT GGTGGTCCAG AATACTCAGG AAATACAACG CGCTCACTGC 
CTACACCAGA CCTTCCATGC ACTGCACAAG TTCCAGCAAC TTCATGGCCG GCTACCCAAG 
CCCTGGGATC CTGATGACGC AGAGACCGTG GTGTGGCTGG CCCAGGACCT AGAGCCACTA 
AAAGGGGCAA AAGAAGAGTC GCTGGATGAG GCTCTACTCC GGACAATTGC CCTGAGTAGT 
GCTGGTAGCT TAAGTCCCAT GGCAGCCATT CTGGGAGGAG TGGCTGCCCA GGAAGTCCTG 
AAGGCAGTCT CCGGGAAGTT CATGCCCCTG GACCAGTGGC TGTACTTTGA TGCCCTTGAA 
TGTCTTCCAG AAGATGAGAC GCTCCTGCCC AGTCCTGAGG ACTGTCATCC AAGAAACTGC 
CGATACGACG GGCAAATTGC TGTGTTCGGG ACTGGTTTTC AGGAGAAACT GAGCTACAAA 
CACTACCTCT TGGTAGGTGC TGGTGCCATT GGCTGTGAGA TGCTCAAAGG TTTTGCCCTA 
GTGGGCCTGG GAGTTAGGGA TAATGGCGGT GTGACAATTG CTGACATGGA CCACGTAGAA 
CGCTCCAACC TCAGCCGGCA GTTCCTCTTC AGGCCCAAGG ACACTGGGAG GCCCAAGGCA 
GAGGTGGCTG CAGAGGCAGC CCATCGCCTA AACCCAGACC TGCAAGTGAC TTCACATACC 
TGCCCTCTGG ATCCCACCAC AGAGGACATC TATGACGACG ACTTCTTCTC CAGGGTGGAC 
GGCGTTGTTG CTGCTTTGGA CAGTTTCCAG GCCCGGCACT ATGTTGCTGC TCGATGCACC 
CACTATTTGA AACCACTGCT GGAGGCAGGC ACCCAGGGAA CCCGGGGGAG TGCTTCAGTG 
TTTGTGCCAT ATGTGACAGA AGTCTACAAA GGTCCTACCT CAGCCGAGGA AGCTCCCTAC 
CCTGTGTGCA CCCTGCGGCA TTTTCCCAGC ACTGTGGAGC ACAGCCTGCA GTGGGCCCAG 
GACGAATTTG AGGGGCTCTT CAGACTATCC GCAGAGACCA TCAACGACTA CCAACAGACA 
TGCACTTCCT CCCTGTTGGG CACCGATGGG ACTGGAATAT TGGCCTTACT GCAGCAAGTG 
ATGGGCGTCC TAAGAACTCG GCCACAGACC TGGCAAGACT GTGTGGTGTG GGCTCTTGGC 
CACTGGCAAC TGTGCTTCCG TGATAACATC GTGGAGCTGC TGAGAAAATT CCCATCTGAT 
AAAGTGCTTG AGGACGGAAC TCTGTTCTGG TCAGGATCCA AAAAGTGCCC ACATCCCTTG 
CAATTTGATC CCAACCAAGA CACGCATTTC CTCTATGTAC TGGCAGCTGC CAACCTGTAT 
GCACGGATGC ATGGGCTGCC GGGCTCACAA AGTCAGACTG CACTCAGGGA ACTGCTGACA 
CTGACACTGC TGCAGGAGCC TGATTCCATG CCCCAGAACC TCTTCTCTGC TGAGTTTGGC 
CAGGAACAGT TGAAGGAACT GCAGGAAACC CTGGATGACT GGAACAAGGG GCCTCCACTG 
AAGCCTGTGC TGTTTGGGAA GGATGACAGC AGCAACTTCC ACGAGGACTT TGTAGTAGCA 
GCGACAGACC TGCGCTGTCA GAACTATGGG ATCCTGCCAG TTAACCGTGC TCGGATCAAG 
CAAACCATCG GCCGGATTAT CCCAGCCATT GCCACCAGTA CAGCAGTTGT GGCGGGCTTA 
CTGGGCCTGG AGCTGTATAA GGTGGTAAGT GGGCCACGGC CCCTTGGCAC CTTTCGGCAC 
AGCTACCTGC ACCTGGCTGA AAACCACTTC ATACGCTCAG CACCTTCTGC CCCGGCCATG 
CAGTCGTTCC ATCACCTGGA ATGGACCTGT TGGCACCGCC TGACGGTGCC TGCTGGGCAG 
CCTGAGAGGA CACTGGAATC CCTGCTGGCC CATCTCCAGG AAGAGCATGG ACTGACGGTG 
AGGATGCTGC TGCATGACCA GGCTCTGCTC TATTCTTCAG GATGGCCATT TGAAAAGCAG 
GCTCAGCACT TGGGCCTCAG ATCTTGCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba7 ( XM_006243743.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243805.1 Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa31558
Clone ID Related Accession (Same CDS sequence)	XM_006243743.3
Accession Version	XM_006243743.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme 7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243743.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCTGTACTC GAGGCAGCTG TATGTACTGG GCTTGCCTGC TATGCAGAGG 
ATCCGGGAAG CCAAAGTCTT GCTGTGTGGC CTGCAGGGCC TGGGAGCTGA GGTGGCCAAG 
AACCTGGTCC TTATGGGTGT GGGCAGCCTC ACCCTGCATG ATCCCCATCC CACCTGCTGG 
GCTGACTTGG CTGCTCAGTT TTTCCTCTCA GAAGAGAGCT TGGGAAGGAG CAGGGCTGAG 
GCCTCTCAAC CACAATTGGC CCAGCTTAAT GAAGCTGTCC AGATCTCCGT CCACAGAGGC 
GACATCACTG AGGACCTAGT GCGGGGTTTT CAGGTAGTGG TGCTGACTGA CTCCAAGCTA 
GAGGACCAGC TGAATATGGG AGCCTTGTGC CATAAGAATA GAGTCTACTT CCTCATGGCT 
GAAACCCGGG GCCTTGTGGG GCGGTTATTC TGTGATTTTG GTGAGGACTT CACTGTTGTG 
GACCCTACAG AGGTGGAGCC CACGTCAGCT GCCATCCAGG ACATCTCTCA GGGCTCCCCC 
GGCATTGTCA CTCTGAGAGG AGGCAGCAAA AGACCTTCCT TTTATGATGG GGACTTGGTG 
ATGTTCTCAG ACATTGAAGG CATGGTTGAA CTCAACAGTC ATTCTCCACA GCCTGTCCGT 
GTGCAGAAAG ATGGATCCTT GGAGATTGGA GATACAACAA CTTTCTCCCG TTACTTGAGG 
GGTGGGGTTG TCACTGAAGT CAAGAGACCC AAGACTGTGA AGCATAAGCC CCTGGACATA 
GCTCTGCTCC AGCCCTGCAT GGTGGTCCAG AATACTCAGG AAATACAACG CGCTCACTGC 
CTACACCAGA CCTTCCATGC ACTGCACAAG TTCCAGCAAC TTCATGGCCG GCTACCCAAG 
CCCTGGGATC CTGATGACGC AGAGACCGTG GTGTGGCTGG CCCAGGACCT AGAGCCACTA 
AAAGGGGCAA AAGAAGAGTC GCTGGATGAG GCTCTACTCC GGACAATTGC CCTGAGTAGT 
GCTGGTAGCT TAAGTCCCAT GGCAGCCATT CTGGGAGGAG TGGCTGCCCA GGAAGTCCTG 
AAGGCAGTCT CCGGGAAGTT CATGCCCCTG GACCAGTGGC TGTACTTTGA TGCCCTTGAA 
TGTCTTCCAG AAGATGAGAC GCTCCTGCCC AGTCCTGAGG ACTGTCATCC AAGAAACTGC 
CGATACGACG GGCAAATTGC TGTGTTCGGG ACTGGTTTTC AGGAGAAACT GAGCTACAAA 
CACTACCTCT TGGTAGGTGC TGGTGCCATT GGCTGTGAGA TGCTCAAAGG TTTTGCCCTA 
GTGGGCCTGG GAGTTAGGGA TAATGGCGGT GTGACAATTG CTGACATGGA CCACGTAGAA 
CGCTCCAACC TCAGCCGGCA GTTCCTCTTC AGGCCCAAGG ACACTGGGAG GCCCAAGGCA 
GAGGTGGCTG CAGAGGCAGC CCATCGCCTA AACCCAGACC TGCAAGTGAC TTCACATACC 
TGCCCTCTGG ATCCCACCAC AGAGGACATC TATGACGACG ACTTCTTCTC CAGGGTGGAC 
GGCGTTGTTG CTGCTTTGGA CAGTTTCCAG GCCCGGCACT ATGTTGCTGC TCGATGCACC 
CACTATTTGA AACCACTGCT GGAGGCAGGC ACCCAGGGAA CCCGGGGGAG TGCTTCAGTG 
TTTGTGCCAT ATGTGACAGA AGTCTACAAA GGTCCTACCT CAGCCGAGGA AGCTCCCTAC 
CCTGTGTGCA CCCTGCGGCA TTTTCCCAGC ACTGTGGAGC ACAGCCTGCA GTGGGCCCAG 
GACGAATTTG AGGGGCTCTT CAGACTATCC GCAGAGACCA TCAACGACTA CCAACAGACA 
TGCACTTCCT CCCTGTTGGG CACCGATGGG ACTGGAATAT TGGCCTTACT GCAGCAAGTG 
ATGGGCGTCC TAAGAACTCG GCCACAGACC TGGCAAGACT GTGTGGTGTG GGCTCTTGGC 
CACTGGCAAC TGTGCTTCCG TGATAACATC GTGGAGCTGC TGAGAAAATT CCCATCTGAT 
AAAGTGCTTG AGGACGGAAC TCTGTTCTGG TCAGGATCCA AAAAGTGCCC ACATCCCTTG 
CAATTTGATC CCAACCAAGA CACGCATTTC CTCTATGTAC TGGCAGCTGC CAACCTGTAT 
GCACGGATGC ATGGGCTGCC GGGCTCACAA AGTCAGACTG CACTCAGGGA ACTGCTGACA 
CTGACACTGC TGCAGGAGCC TGATTCCATG CCCCAGAACC TCTTCTCTGC TGAGTTTGGC 
CAGGAACAGT TGAAGGAACT GCAGGAAACC CTGGATGACT GGAACAAGGG GCCTCCACTG 
AAGCCTGTGC TGTTTGGGAA GGCTGTGGAA GCAGCTCAGT CCCCTTGCAC GTCGGTCACA 
CTGTGTGGCA AAGGAGGGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243805.1
CDS	122..2545
Translation	MDEELYSRQLYVLGLPAMQRIREAKVLLCGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWADLAAQFFLSEESLGRSRAEASQPQLAQLNEAVQISVHRGDITEDLVRGFQVVVLTDSKLEDQLNMGALCHKNRVYFLMAETRGLVGRLFCDFGEDFTVVDPTEVEPTSAAIQDISQGSPGIVTLRGGSKRPSFYDGDLVMFSDIEGMVELNSHSPQPVRVQKDGSLEIGDTTTFSRYLRGGVVTEVKRPKTVKHKPLDIALLQPCMVVQNTQEIQRAHCLHQTFHALHKFQQLHGRLPKPWDPDDAETVVWLAQDLEPLKGAKEESLDEALLRTIALSSAGSLSPMAAILGGVAAQEVLKAVSGKFMPLDQWLYFDALECLPEDETLLPSPEDCHPRNCRYDGQIAVFGTGFQEKLSYKHYLLVGAGAIGCEMLKGFALVGLGVRDNGGVTIADMDHVERSNLSRQFLFRPKDTGRPKAEVAAEAAHRLNPDLQVTSHTCPLDPTTEDIYDDDFFSRVDGVVAALDSFQARHYVAARCTHYLKPLLEAGTQGTRGSASVFVPYVTEVYKGPTSAEEAPYPVCTLRHFPSTVEHSLQWAQDEFEGLFRLSAETINDYQQTCTSSLLGTDGTGILALLQQVMGVLRTRPQTWQDCVVWALGHWQLCFRDNIVELLRKFPSDKVLEDGTLFWSGSKKCPHPLQFDPNQDTHFLYVLAAANLYARMHGLPGSQSQTALRELLTLTLLQEPDSMPQNLFSAEFGQEQLKELQETLDDWNKGPPLKPVLFGKAVEAAQSPCTSVTLCGKGGR

Target ORF information:

RefSeq Version	XM_006243743.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243743.3

ORF Insert Sequence:

ATGGATGAAG AGCTGTACTC GAGGCAGCTG TATGTACTGG GCTTGCCTGC TATGCAGAGG 
ATCCGGGAAG CCAAAGTCTT GCTGTGTGGC CTGCAGGGCC TGGGAGCTGA GGTGGCCAAG 
AACCTGGTCC TTATGGGTGT GGGCAGCCTC ACCCTGCATG ATCCCCATCC CACCTGCTGG 
GCTGACTTGG CTGCTCAGTT TTTCCTCTCA GAAGAGAGCT TGGGAAGGAG CAGGGCTGAG 
GCCTCTCAAC CACAATTGGC CCAGCTTAAT GAAGCTGTCC AGATCTCCGT CCACAGAGGC 
GACATCACTG AGGACCTAGT GCGGGGTTTT CAGGTAGTGG TGCTGACTGA CTCCAAGCTA 
GAGGACCAGC TGAATATGGG AGCCTTGTGC CATAAGAATA GAGTCTACTT CCTCATGGCT 
GAAACCCGGG GCCTTGTGGG GCGGTTATTC TGTGATTTTG GTGAGGACTT CACTGTTGTG 
GACCCTACAG AGGTGGAGCC CACGTCAGCT GCCATCCAGG ACATCTCTCA GGGCTCCCCC 
GGCATTGTCA CTCTGAGAGG AGGCAGCAAA AGACCTTCCT TTTATGATGG GGACTTGGTG 
ATGTTCTCAG ACATTGAAGG CATGGTTGAA CTCAACAGTC ATTCTCCACA GCCTGTCCGT 
GTGCAGAAAG ATGGATCCTT GGAGATTGGA GATACAACAA CTTTCTCCCG TTACTTGAGG 
GGTGGGGTTG TCACTGAAGT CAAGAGACCC AAGACTGTGA AGCATAAGCC CCTGGACATA 
GCTCTGCTCC AGCCCTGCAT GGTGGTCCAG AATACTCAGG AAATACAACG CGCTCACTGC 
CTACACCAGA CCTTCCATGC ACTGCACAAG TTCCAGCAAC TTCATGGCCG GCTACCCAAG 
CCCTGGGATC CTGATGACGC AGAGACCGTG GTGTGGCTGG CCCAGGACCT AGAGCCACTA 
AAAGGGGCAA AAGAAGAGTC GCTGGATGAG GCTCTACTCC GGACAATTGC CCTGAGTAGT 
GCTGGTAGCT TAAGTCCCAT GGCAGCCATT CTGGGAGGAG TGGCTGCCCA GGAAGTCCTG 
AAGGCAGTCT CCGGGAAGTT CATGCCCCTG GACCAGTGGC TGTACTTTGA TGCCCTTGAA 
TGTCTTCCAG AAGATGAGAC GCTCCTGCCC AGTCCTGAGG ACTGTCATCC AAGAAACTGC 
CGATACGACG GGCAAATTGC TGTGTTCGGG ACTGGTTTTC AGGAGAAACT GAGCTACAAA 
CACTACCTCT TGGTAGGTGC TGGTGCCATT GGCTGTGAGA TGCTCAAAGG TTTTGCCCTA 
GTGGGCCTGG GAGTTAGGGA TAATGGCGGT GTGACAATTG CTGACATGGA CCACGTAGAA 
CGCTCCAACC TCAGCCGGCA GTTCCTCTTC AGGCCCAAGG ACACTGGGAG GCCCAAGGCA 
GAGGTGGCTG CAGAGGCAGC CCATCGCCTA AACCCAGACC TGCAAGTGAC TTCACATACC 
TGCCCTCTGG ATCCCACCAC AGAGGACATC TATGACGACG ACTTCTTCTC CAGGGTGGAC 
GGCGTTGTTG CTGCTTTGGA CAGTTTCCAG GCCCGGCACT ATGTTGCTGC TCGATGCACC 
CACTATTTGA AACCACTGCT GGAGGCAGGC ACCCAGGGAA CCCGGGGGAG TGCTTCAGTG 
TTTGTGCCAT ATGTGACAGA AGTCTACAAA GGTCCTACCT CAGCCGAGGA AGCTCCCTAC 
CCTGTGTGCA CCCTGCGGCA TTTTCCCAGC ACTGTGGAGC ACAGCCTGCA GTGGGCCCAG 
GACGAATTTG AGGGGCTCTT CAGACTATCC GCAGAGACCA TCAACGACTA CCAACAGACA 
TGCACTTCCT CCCTGTTGGG CACCGATGGG ACTGGAATAT TGGCCTTACT GCAGCAAGTG 
ATGGGCGTCC TAAGAACTCG GCCACAGACC TGGCAAGACT GTGTGGTGTG GGCTCTTGGC 
CACTGGCAAC TGTGCTTCCG TGATAACATC GTGGAGCTGC TGAGAAAATT CCCATCTGAT 
AAAGTGCTTG AGGACGGAAC TCTGTTCTGG TCAGGATCCA AAAAGTGCCC ACATCCCTTG 
CAATTTGATC CCAACCAAGA CACGCATTTC CTCTATGTAC TGGCAGCTGC CAACCTGTAT 
GCACGGATGC ATGGGCTGCC GGGCTCACAA AGTCAGACTG CACTCAGGGA ACTGCTGACA 
CTGACACTGC TGCAGGAGCC TGATTCCATG CCCCAGAACC TCTTCTCTGC TGAGTTTGGC 
CAGGAACAGT TGAAGGAACT GCAGGAAACC CTGGATGACT GGAACAAGGG GCCTCCACTG 
AAGCCTGTGC TGTTTGGGAA GGCTGTGGAA GCAGCTCAGT CCCCTTGCAC GTCGGTCACA 
CTGTGTGGCA AAGGAGGGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uba7 ( XM_008766505.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764727.1 Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X3, mRNA.

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CloneID	ORa45774
Clone ID Related Accession (Same CDS sequence)	XM_008766505.2
Accession Version	XM_008766505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-like modifier-activating enzyme 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCCT TGTGCCATAA GAATAGAGTC TACTTCCTCA TGGCTGAAAC CCGGGGCCTT 
GTGGGGCGGT TATTCTGTGA TTTTGGTGAG GACTTCACTG TTGTGGACCC TACAGAGGTG 
GAGCCCACGT CAGCTGCCAT CCAGGACATC TCTCAGGGCT CCCCCGGCAT TGTCACTCTG 
AGAGGAGGCA GCAAAAGACC TTCCTTTTAT GATGGGGACT TGGTGATGTT CTCAGACATT 
GAAGGCATGG TTGAACTCAA CAGTCATTCT CCACAGCCTG TCCGTGTGCA GAAAGATGGA 
TCCTTGGAGA TTGGAGATAC AACAACTTTC TCCCGTTACT TGAGGGGTGG GGTTGTCACT 
GAAGTCAAGA GACCCAAGAC TGTGAAGCAT AAGCCCCTGG ACATAGCTCT GCTCCAGCCC 
TGCATGGTGG TCCAGAATAC TCAGGAAATA CAACGCGCTC ACTGCCTACA CCAGACCTTC 
CATGCACTGC ACAAGTTCCA GCAACTTCAT GGCCGGCTAC CCAAGCCCTG GGATCCTGAT 
GACGCAGAGA CCGTGGTGTG GCTGGCCCAG GACCTAGAGC CACTAAAAGG GGCAAAAGAA 
GAGTCGCTGG ATGAGGCTCT ACTCCGGACA ATTGCCCTGA GTAGTGCTGG TAGCTTAAGT 
CCCATGGCAG CCATTCTGGG AGGAGTGGCT GCCCAGGAAG TCCTGAAGGC AGTCTCCGGG 
AAGTTCATGC CCCTGGACCA GTGGCTGTAC TTTGATGCCC TTGAATGTCT TCCAGAAGAT 
GAGACGCTCC TGCCCAGTCC TGAGGACTGT CATCCAAGAA ACTGCCGATA CGACGGGCAA 
ATTGCTGTGT TCGGGACTGG TTTTCAGGAG AAACTGAGCT ACAAACACTA CCTCTTGGTA 
GGTGCTGGTG CCATTGGCTG TGAGATGCTC AAAGGTTTTG CCCTAGTGGG CCTGGGAGTT 
AGGGATAATG GCGGTGTGAC AATTGCTGAC ATGGACCACG TAGAACGCTC CAACCTCAGC 
CGGCAGTTCC TCTTCAGGCC CAAGGACACT GGGAGGCCCA AGGCAGAGGT GGCTGCAGAG 
GCAGCCCATC GCCTAAACCC AGACCTGCAA GTGACTTCAC ATACCTGCCC TCTGGATCCC 
ACCACAGAGG ACATCTATGA CGACGACTTC TTCTCCAGGG TGGACGGCGT TGTTGCTGCT 
TTGGACAGTT TCCAGGCCCG GCACTATGTT GCTGCTCGAT GCACCCACTA TTTGAAACCA 
CTGCTGGAGG CAGGCACCCA GGGAACCCGG GGGAGTGCTT CAGTGTTTGT GCCATATGTG 
ACAGAAGTCT ACAAAGGTCC TACCTCAGCC GAGGAAGCTC CCTACCCTGT GTGCACCCTG 
CGGCATTTTC CCAGCACTGT GGAGCACAGC CTGCAGTGGG CCCAGGACGA ATTTGAGGGG 
CTCTTCAGAC TATCCGCAGA GACCATCAAC GACTACCAAC AGACATGCAC TTCCTCCCTG 
TTGGGCACCG ATGGGACTGG AATATTGGCC TTACTGCAGC AAGTGATGGG CGTCCTAAGA 
ACTCGGCCAC AGACCTGGCA AGACTGTGTG GTGTGGGCTC TTGGCCACTG GCAACTGTGC 
TTCCGTGATA ACATCGTGGA GCTGCTGAGA AAATTCCCAT CTGATAAAGT GCTTGAGGAC 
GGAACTCTGT TCTGGTCAGG ATCCAAAAAG TGCCCACATC CCTTGCAATT TGATCCCAAC 
CAAGACACGC ATTTCCTCTA TGTACTGGCA GCTGCCAACC TGTATGCACG GATGCATGGG 
CTGCCGGGCT CACAAAGTCA GACTGCACTC AGGGAACTGC TGACACTGAC ACTGCTGCAG 
GAGCCTGATT CCATGCCCCA GAACCTCTTC TCTGCTGAGT TTGGCCAGGA ACAGTTGAAG 
GAACTGCAGG AAACCCTGGA TGACTGGAAC AAGGGGCCTC CACTGAAGCC TGTGCTGTTT 
GGGAAGGATG ACAGCAGCAA CTTCCACGAG GACTTTGTAG TAGCAGCGAC AGACCTGCGC 
TGTCAGAACT ATGGGATCCT GCCAGTTAAC CGTGCTCGGA TCAAGCAAAC CATCGGCCGG 
ATTATCCCAG CCATTGCCAC CAGTACAGCA GTTGTGGCGG GCTTACTGGG CCTGGAGCTG 
TATAAGGTGG TAAGTGGGCC ACGGCCCCTT GGCACCTTTC GGCACAGCTA CCTGCACCTG 
GCTGAAAACC ACTTCATACG CTCAGCACCT TCTGCCCCGG CCATGCAGTC GTTCCATCAC 
CTGGAATGGA CCTGTTGGCA CCGCCTGACG GTGCCTGCTG GGCAGCCTGA GAGGACACTG 
GAATCCCTGC TGGCCCATCT CCAGGAAGAG CATGGACTGA CGGTGAGGAT GCTGCTGCAT 
GACCAGGCTC TGCTCTATTC TTCAGGATGG CCATTTGAAA AGCAGGCTCA GCACTTGGGC 
CTCAGGGTGA CAGAACTAGT TCAGCAGGTG ACCGGCCAGA AACCCAAGCC TGGGCTGAGA 
GTTCTGGTGT TTGAGCTGAG CTGTGAGGGT GAGGATGACG AAATGGCCTT CCCACCTCTG 
CATTATGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764727.1
CDS	247..2841
Translation	MGALCHKNRVYFLMAETRGLVGRLFCDFGEDFTVVDPTEVEPTSAAIQDISQGSPGIVTLRGGSKRPSFYDGDLVMFSDIEGMVELNSHSPQPVRVQKDGSLEIGDTTTFSRYLRGGVVTEVKRPKTVKHKPLDIALLQPCMVVQNTQEIQRAHCLHQTFHALHKFQQLHGRLPKPWDPDDAETVVWLAQDLEPLKGAKEESLDEALLRTIALSSAGSLSPMAAILGGVAAQEVLKAVSGKFMPLDQWLYFDALECLPEDETLLPSPEDCHPRNCRYDGQIAVFGTGFQEKLSYKHYLLVGAGAIGCEMLKGFALVGLGVRDNGGVTIADMDHVERSNLSRQFLFRPKDTGRPKAEVAAEAAHRLNPDLQVTSHTCPLDPTTEDIYDDDFFSRVDGVVAALDSFQARHYVAARCTHYLKPLLEAGTQGTRGSASVFVPYVTEVYKGPTSAEEAPYPVCTLRHFPSTVEHSLQWAQDEFEGLFRLSAETINDYQQTCTSSLLGTDGTGILALLQQVMGVLRTRPQTWQDCVVWALGHWQLCFRDNIVELLRKFPSDKVLEDGTLFWSGSKKCPHPLQFDPNQDTHFLYVLAAANLYARMHGLPGSQSQTALRELLTLTLLQEPDSMPQNLFSAEFGQEQLKELQETLDDWNKGPPLKPVLFGKDDSSNFHEDFVVAATDLRCQNYGILPVNRARIKQTIGRIIPAIATSTAVVAGLLGLELYKVVSGPRPLGTFRHSYLHLAENHFIRSAPSAPAMQSFHHLEWTCWHRLTVPAGQPERTLESLLAHLQEEHGLTVRMLLHDQALLYSSGWPFEKQAQHLGLRVTELVQQVTGQKPKPGLRVLVFELSCEGEDDEMAFPPLHYEL

Target ORF information:

RefSeq Version	XM_008766505.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-like modifier activating enzyme 7 (Uba7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766505.2

ORF Insert Sequence:

ATGGGAGCCT TGTGCCATAA GAATAGAGTC TACTTCCTCA TGGCTGAAAC CCGGGGCCTT 
GTGGGGCGGT TATTCTGTGA TTTTGGTGAG GACTTCACTG TTGTGGACCC TACAGAGGTG 
GAGCCCACGT CAGCTGCCAT CCAGGACATC TCTCAGGGCT CCCCCGGCAT TGTCACTCTG 
AGAGGAGGCA GCAAAAGACC TTCCTTTTAT GATGGGGACT TGGTGATGTT CTCAGACATT 
GAAGGCATGG TTGAACTCAA CAGTCATTCT CCACAGCCTG TCCGTGTGCA GAAAGATGGA 
TCCTTGGAGA TTGGAGATAC AACAACTTTC TCCCGTTACT TGAGGGGTGG GGTTGTCACT 
GAAGTCAAGA GACCCAAGAC TGTGAAGCAT AAGCCCCTGG ACATAGCTCT GCTCCAGCCC 
TGCATGGTGG TCCAGAATAC TCAGGAAATA CAACGCGCTC ACTGCCTACA CCAGACCTTC 
CATGCACTGC ACAAGTTCCA GCAACTTCAT GGCCGGCTAC CCAAGCCCTG GGATCCTGAT 
GACGCAGAGA CCGTGGTGTG GCTGGCCCAG GACCTAGAGC CACTAAAAGG GGCAAAAGAA 
GAGTCGCTGG ATGAGGCTCT ACTCCGGACA ATTGCCCTGA GTAGTGCTGG TAGCTTAAGT 
CCCATGGCAG CCATTCTGGG AGGAGTGGCT GCCCAGGAAG TCCTGAAGGC AGTCTCCGGG 
AAGTTCATGC CCCTGGACCA GTGGCTGTAC TTTGATGCCC TTGAATGTCT TCCAGAAGAT 
GAGACGCTCC TGCCCAGTCC TGAGGACTGT CATCCAAGAA ACTGCCGATA CGACGGGCAA 
ATTGCTGTGT TCGGGACTGG TTTTCAGGAG AAACTGAGCT ACAAACACTA CCTCTTGGTA 
GGTGCTGGTG CCATTGGCTG TGAGATGCTC AAAGGTTTTG CCCTAGTGGG CCTGGGAGTT 
AGGGATAATG GCGGTGTGAC AATTGCTGAC ATGGACCACG TAGAACGCTC CAACCTCAGC 
CGGCAGTTCC TCTTCAGGCC CAAGGACACT GGGAGGCCCA AGGCAGAGGT GGCTGCAGAG 
GCAGCCCATC GCCTAAACCC AGACCTGCAA GTGACTTCAC ATACCTGCCC TCTGGATCCC 
ACCACAGAGG ACATCTATGA CGACGACTTC TTCTCCAGGG TGGACGGCGT TGTTGCTGCT 
TTGGACAGTT TCCAGGCCCG GCACTATGTT GCTGCTCGAT GCACCCACTA TTTGAAACCA 
CTGCTGGAGG CAGGCACCCA GGGAACCCGG GGGAGTGCTT CAGTGTTTGT GCCATATGTG 
ACAGAAGTCT ACAAAGGTCC TACCTCAGCC GAGGAAGCTC CCTACCCTGT GTGCACCCTG 
CGGCATTTTC CCAGCACTGT GGAGCACAGC CTGCAGTGGG CCCAGGACGA ATTTGAGGGG 
CTCTTCAGAC TATCCGCAGA GACCATCAAC GACTACCAAC AGACATGCAC TTCCTCCCTG 
TTGGGCACCG ATGGGACTGG AATATTGGCC TTACTGCAGC AAGTGATGGG CGTCCTAAGA 
ACTCGGCCAC AGACCTGGCA AGACTGTGTG GTGTGGGCTC TTGGCCACTG GCAACTGTGC 
TTCCGTGATA ACATCGTGGA GCTGCTGAGA AAATTCCCAT CTGATAAAGT GCTTGAGGAC 
GGAACTCTGT TCTGGTCAGG ATCCAAAAAG TGCCCACATC CCTTGCAATT TGATCCCAAC 
CAAGACACGC ATTTCCTCTA TGTACTGGCA GCTGCCAACC TGTATGCACG GATGCATGGG 
CTGCCGGGCT CACAAAGTCA GACTGCACTC AGGGAACTGC TGACACTGAC ACTGCTGCAG 
GAGCCTGATT CCATGCCCCA GAACCTCTTC TCTGCTGAGT TTGGCCAGGA ACAGTTGAAG 
GAACTGCAGG AAACCCTGGA TGACTGGAAC AAGGGGCCTC CACTGAAGCC TGTGCTGTTT 
GGGAAGGATG ACAGCAGCAA CTTCCACGAG GACTTTGTAG TAGCAGCGAC AGACCTGCGC 
TGTCAGAACT ATGGGATCCT GCCAGTTAAC CGTGCTCGGA TCAAGCAAAC CATCGGCCGG 
ATTATCCCAG CCATTGCCAC CAGTACAGCA GTTGTGGCGG GCTTACTGGG CCTGGAGCTG 
TATAAGGTGG TAAGTGGGCC ACGGCCCCTT GGCACCTTTC GGCACAGCTA CCTGCACCTG 
GCTGAAAACC ACTTCATACG CTCAGCACCT TCTGCCCCGG CCATGCAGTC GTTCCATCAC 
CTGGAATGGA CCTGTTGGCA CCGCCTGACG GTGCCTGCTG GGCAGCCTGA GAGGACACTG 
GAATCCCTGC TGGCCCATCT CCAGGAAGAG CATGGACTGA CGGTGAGGAT GCTGCTGCAT 
GACCAGGCTC TGCTCTATTC TTCAGGATGG CCATTTGAAA AGCAGGCTCA GCACTTGGGC 
CTCAGGGTGA CAGAACTAGT TCAGCAGGTG ACCGGCCAGA AACCCAAGCC TGGGCTGAGA 
GTTCTGGTGT TTGAGCTGAG CTGTGAGGGT GAGGATGACG AAATGGCCTT CCCACCTCTG 
CATTATGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ISG15 Arg151 and the ISG15-conjugating enzyme UbE1L are important for innate immune control of Sindbis virus.
Journal of virology83(4)1602-10(2009 Feb)
Giannakopoulos NV,Arutyunova E,Lai C,Lenschow DJ,Haas AL,Virgin HW

The basis for selective E1-E2 interactions in the ISG15 conjugation system.
The Journal of biological chemistry283(35)23895-902(2008 Aug)
Durfee LA,Kelley ML,Huibregtse JM

Ube1L and protein ISGylation are not essential for alpha/beta interferon signaling.
Molecular and cellular biology26(2)472-9(2006 Jan)
Kim KI,Yan M,Malakhova O,Luo JK,Shen MF,Zou W,de la Torre JC,Zhang DE

Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme.
Journal of biochemistry138(6)711-9(2005 Dec)
Takeuchi T,Iwahara S,Saeki Y,Sasajima H,Yokosawa H

Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.
Biochemical and biophysical research communications336(2)496-506(2005 Oct)
Giannakopoulos NV,Luo JK,Papov V,Zou W,Lenschow DJ,Jacobs BS,Borden EC,Li J,Virgin HW,Zhang DE

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Uba7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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