Lima1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lima1
Entrez Gene ID	300228
Full Name	LIM domain and actin binding 1
Synonyms	eplin
General protein information	Preferred Names LIM domain and actin binding 1 Names LIM domain and actin-binding protein 1 epithelial protein lost in neoplasm
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_006257355.3	XP_006257417.1	LIM domain and actin-binding protein 1 isoform X2
NM_001191615.2	NP_001178544.1	LIM domain and actin-binding protein 1
XM_006257354.3	XP_006257416.1	LIM domain and actin-binding protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LIMA1	XP_005636908.1
Pan troglodytes (chimpanzee)	LIMA1	XP_001154716.2
Mus musculus (house mouse)	Lima1	NP_001107017.1
Xenopus tropicalis (tropical clawed frog)	lima1	NP_001072345.1
Gallus gallus (chicken)	LIMA1	XP_004949785.1
Homo sapiens (human)	LIMA1	NP_057441.1
Macaca mulatta (Rhesus monkey)	LIMA1	XP_001102909.1
Bos taurus (cattle)	LIMA1	NP_001179683.1
Rattus norvegicus (Norway rat)	Lima1	NP_001178544.1
Danio rerio (zebrafish)	im:7155904	XP_005172858.1

Gene Ontology
Bibliography

Related articles in PubMed

A
Zhang YY, Fu ZY, Wei J, Qi W, Baituola G, Luo J, Meng YJ, Guo SY, Yin H, Jiang SY, Li YF, Miao HH, Liu Y, Wang Y, Li BL, Ma YT, Song BL
Science (New York, N.Y.)360(6393)1087-1092(2018 06)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Epithelial protein lost in neoplasm modulates platelet-derived growth factor-mediated adhesion and motility of mesangial cells.
Tsurumi H, Harita Y, Kurihara H, Kosako H, Hayashi K, Matsunaga A, Kajiho Y, Kanda S, Miura K, Sekine T, Oka A, Ishizuka K, Horita S, Hattori M, Hattori S, Igarashi T
Kidney international86(3)548-57(2014 Sep)

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Revenu C, Ubelmann F, Hurbain I, El-Marjou F, Dingli F, Loew D, Delacour D, Gilet J, Brot-Laroche E, Rivero F, Louvard D, Robine S
Molecular biology of the cell23(2)324-36(2012 Jan)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lima1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lima1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07182	NM_001191615.2 Latest version!	Rattus norvegicus LIM domain and actin binding 1 (Lima1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa07182	XM_006257355.3 Latest version!	Rattus norvegicus LIM domain and actin binding 1 (Lima1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa40128	XM_006257354.3 Latest version!	Rattus norvegicus LIM domain and actin binding 1 (Lima1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lima1 ( NM_001191615.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178544.1 Rattus norvegicus LIM domain and actin binding 1 (Lima1), mRNA.

Price & Availability↑

CloneID	ORa07182
Clone ID Related Accession (Same CDS sequence)	XM_006257355.3 , NM_001191615.2
Accession Version	NM_001191615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain and actin-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07058877.1, AABR07058876.1, AABR07058875.1, AABR07058874.1 and AABR07073547.1. On Sep 9, 2010 this sequence version replaced NM_001191615.1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. There are no full-length rat transcripts representing this gene; the genomic coordinates used for the transcript record are predicted based on partial rat transcript alignments and on full-length protein alignments with mouse NP_001107017.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATCGA CTCCGTTTAA TAGACGGCAG TGGACTTCCC TGTCACTGAG AGTAACAGCC 
AAAGAACTTT CTCTCGTCAA CAAGAACAAG TCGTCCACAA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAGGAAGA AAAATAACAC TGAAAGTCTC 
CCGCAGCACT TTAGAAGAGG GACCCTGTCT GTGTTAAAGA AGAAGTGGGA GAACCCCGTG 
GCGGGAGCGG AATCCCACAC AGACTCACTG CCAAACAGCA GCAGCGACGG TGGGCACACG 
GCGGACCACC CTCCTGCTGA AGTGACAGCC AAGGCTGCTC CTGGAGCCAG AGCTGACCGG 
GAAGAGCACA CCCAGCCCAG ATCTAGATTT GGATCTCGTC CGGAAGCTGT TACTCAGTGT 
CGGTATCCCC GCTCAGAGGA CAGCCACGAT TTTAAAGCCC AGGCCACGGA AAGCCAAAAT 
ATGGAAAACT GTCTGGGAGA TTCCAGGCAT GAAGCAGAGA AGCCCGAAAT GAGCGAAAAC 
ACGGAAACTT CAGGCAAAAT AGAGAAATAC AACGTTCCAC TGAACAGACT AAAGATGATG 
TTTGAGAAAG GTGAACACAG CCAAAACAAG AGTCCCTGGA CCCAGGGCCG AAATGCAGGC 
GGAAGGAGGC TCTCTGAAAA CAGCTGTTCC CTGGACGATT TGGAAATAGG TGCAGGTCAT 
TTGTCTTCCT CTGCCTTCAA CTCGGAGAAA AATGAGAGTA AGCGGAATCT GGAGCTGCCA 
CGCCTCTCTG AGACCTCCAT AAAGGACCGC ATGGCCAAGT ACCAGGCTGC CGTGTCCAAG 
CAGAGCAGCC CAGCCAGCTA CGCAAGTGAG CTGAAACCCA GTGAAAGCAA AACTCACAAA 
TGGGAGCAGA AGGAGAACGT GCCCCCCGGC CCCGAGGCCT GCAGCATCCA TCAGGAAGGG 
AGCAAGGTTT CTGCAACTGA GAATAGCCTG GTGGCCCACC CTGTCCCTGC AGAAGATGAC 
ACCTGTAACT CCCAGGGGAG GAGTGAGGCC CAGCAGCCCA TATACACTAA GCCGCTGAGT 
CCCGACGCCA GGACCTCCAG TCTTCCGGAA AGTTCTCCTT CCAAAACAGC GAAGAAGTTT 
CAGGCACCCG CAAGAGAGAG CTGCGTGGAG TGTCAGAAGA CGGTGTATCC CATGGAACGG 
CTCCTGGCCA ACCAGCAGGT GTTTCACATC AGCTGTTTCC GATGCTCCTA CTGCAACAAC 
AAACTCAGTC TAGGAACATA TGCATCCTTA CACGGAAGAA TCTATTGTAA GCCTCACTTC 
AACCAACTCT TCAAGTCCAA AGGCAACTAT GACGAAGGCT TTGGGCACAA GCAGCATAAG 
GATCTGTGGG CAAGCAAAGG TGAAAATGAA GAGACTTTGG GGAGACCAGC CCAGCCTCCC 
AGTGCAGGGG AGACCCCCCA TAGCCCGGGT GTAGAAGATG CCCCCATCGC CAAGGTCGGC 
GTGCTGGCTG CGAGTATGGA AGCCAAGGCC TCCTCTCAGA GGGAGAGGGA AGAGAATAAG 
CCAGCGGAGA CCAAGAAGCT GAGGATCGCC TGGCCTCCCC CAGCCGAGCA GGGCAGTTCA 
GGAAGTGCCC CGGAGGAAGG GTTCAAAGTG TCAAAGCCCA AGTGGCCTCC CGAGGATGAA 
GTCTGCAAGA CAGAGGCGCC GGAGGATGTA GATCTGGACC TGAAGAAGCT GAGGCGCTCT 
TCGTCCCTGA AGGAGAGGAG CCGCCCGTTT ACTGTCGCGG CTTCCTTTCG AACCTCCTCG 
GTCAAGAGCC CCAAGCCCTT GTCGCCGTCC CTCAGGAAGG GTTGGAGCGA GCCGGAGCCC 
GAGCAGAGTG AGGAGTTCGG AGGAGGGACA GTGACACAAA CGGAAAGCCC CAGGCCCTCT 
CGGGAGAAGG AGAGTGTGGG AAAATCACGC TGGCAGAGCG AGGAAGCGGA AGCGGAAGCG 
GAGGAAGCGC CGAGGGGCAG GGACGGTCGT TCTTTTGAGC TGGAGAGTGA GAGTTTTATA 
GGAAATGGCG CAAGCATAGC TGAAGACGAC GTCGCCCCAG CACAGCGGTC TCCATTAGAA 
CCCGAGTCTC CGGGTTGGCC TGGCTTTGGA GACACCACGA CTGCTAAAGA ATTCAATCAA 
AAATCCCAAG ATGTCGGGTT CTGGGAGGGG GAAGTGGTCC GAGAGCTGTC CGTGGAGGAG 
CAGATAAAGA GAAACCGGTA CTATGACGAG GATGAGGATG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178544.1
CDS	1..2268
Translation	MESTPFNRRQWTSLSLRVTAKELSLVNKNKSSTIVEIFSKYQKAAEEANMERKKNNTESLPQHFRRGTLSVLKKKWENPVAGAESHTDSLPNSSSDGGHTADHPPAEVTAKAAPGARADREEHTQPRSRFGSRPEAVTQCRYPRSEDSHDFKAQATESQNMENCLGDSRHEAEKPEMSENTETSGKIEKYNVPLNRLKMMFEKGEHSQNKSPWTQGRNAGGRRLSENSCSLDDLEIGAGHLSSSAFNSEKNESKRNLELPRLSETSIKDRMAKYQAAVSKQSSPASYASELKPSESKTHKWEQKENVPPGPEACSIHQEGSKVSATENSLVAHPVPAEDDTCNSQGRSEAQQPIYTKPLSPDARTSSLPESSPSKTAKKFQAPARESCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHKQHKDLWASKGENEETLGRPAQPPSAGETPHSPGVEDAPIAKVGVLAASMEAKASSQREREENKPAETKKLRIAWPPPAEQGSSGSAPEEGFKVSKPKWPPEDEVCKTEAPEDVDLDLKKLRRSSSLKERSRPFTVAASFRTSSVKSPKPLSPSLRKGWSEPEPEQSEEFGGGTVTQTESPRPSREKESVGKSRWQSEEAEAEAEEAPRGRDGRSFELESESFIGNGASIAEDDVAPAQRSPLEPESPGWPGFGDTTTAKEFNQKSQDVGFWEGEVVRELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	NM_001191615.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain and actin binding 1 (Lima1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191615.2

ORF Insert Sequence:

ATGGAATCGA CTCCGTTTAA TAGACGGCAG TGGACTTCCC TGTCACTGAG AGTAACAGCC 
AAAGAACTTT CTCTCGTCAA CAAGAACAAG TCGTCCACAA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAGGAAGA AAAATAACAC TGAAAGTCTC 
CCGCAGCACT TTAGAAGAGG GACCCTGTCT GTGTTAAAGA AGAAGTGGGA GAACCCCGTG 
GCGGGAGCGG AATCCCACAC AGACTCACTG CCAAACAGCA GCAGCGACGG TGGGCACACG 
GCGGACCACC CTCCTGCTGA AGTGACAGCC AAGGCTGCTC CTGGAGCCAG AGCTGACCGG 
GAAGAGCACA CCCAGCCCAG ATCTAGATTT GGATCTCGTC CGGAAGCTGT TACTCAGTGT 
CGGTATCCCC GCTCAGAGGA CAGCCACGAT TTTAAAGCCC AGGCCACGGA AAGCCAAAAT 
ATGGAAAACT GTCTGGGAGA TTCCAGGCAT GAAGCAGAGA AGCCCGAAAT GAGCGAAAAC 
ACGGAAACTT CAGGCAAAAT AGAGAAATAC AACGTTCCAC TGAACAGACT AAAGATGATG 
TTTGAGAAAG GTGAACACAG CCAAAACAAG AGTCCCTGGA CCCAGGGCCG AAATGCAGGC 
GGAAGGAGGC TCTCTGAAAA CAGCTGTTCC CTGGACGATT TGGAAATAGG TGCAGGTCAT 
TTGTCTTCCT CTGCCTTCAA CTCGGAGAAA AATGAGAGTA AGCGGAATCT GGAGCTGCCA 
CGCCTCTCTG AGACCTCCAT AAAGGACCGC ATGGCCAAGT ACCAGGCTGC CGTGTCCAAG 
CAGAGCAGCC CAGCCAGCTA CGCAAGTGAG CTGAAACCCA GTGAAAGCAA AACTCACAAA 
TGGGAGCAGA AGGAGAACGT GCCCCCCGGC CCCGAGGCCT GCAGCATCCA TCAGGAAGGG 
AGCAAGGTTT CTGCAACTGA GAATAGCCTG GTGGCCCACC CTGTCCCTGC AGAAGATGAC 
ACCTGTAACT CCCAGGGGAG GAGTGAGGCC CAGCAGCCCA TATACACTAA GCCGCTGAGT 
CCCGACGCCA GGACCTCCAG TCTTCCGGAA AGTTCTCCTT CCAAAACAGC GAAGAAGTTT 
CAGGCACCCG CAAGAGAGAG CTGCGTGGAG TGTCAGAAGA CGGTGTATCC CATGGAACGG 
CTCCTGGCCA ACCAGCAGGT GTTTCACATC AGCTGTTTCC GATGCTCCTA CTGCAACAAC 
AAACTCAGTC TAGGAACATA TGCATCCTTA CACGGAAGAA TCTATTGTAA GCCTCACTTC 
AACCAACTCT TCAAGTCCAA AGGCAACTAT GACGAAGGCT TTGGGCACAA GCAGCATAAG 
GATCTGTGGG CAAGCAAAGG TGAAAATGAA GAGACTTTGG GGAGACCAGC CCAGCCTCCC 
AGTGCAGGGG AGACCCCCCA TAGCCCGGGT GTAGAAGATG CCCCCATCGC CAAGGTCGGC 
GTGCTGGCTG CGAGTATGGA AGCCAAGGCC TCCTCTCAGA GGGAGAGGGA AGAGAATAAG 
CCAGCGGAGA CCAAGAAGCT GAGGATCGCC TGGCCTCCCC CAGCCGAGCA GGGCAGTTCA 
GGAAGTGCCC CGGAGGAAGG GTTCAAAGTG TCAAAGCCCA AGTGGCCTCC CGAGGATGAA 
GTCTGCAAGA CAGAGGCGCC GGAGGATGTA GATCTGGACC TGAAGAAGCT GAGGCGCTCT 
TCGTCCCTGA AGGAGAGGAG CCGCCCGTTT ACTGTCGCGG CTTCCTTTCG AACCTCCTCG 
GTCAAGAGCC CCAAGCCCTT GTCGCCGTCC CTCAGGAAGG GTTGGAGCGA GCCGGAGCCC 
GAGCAGAGTG AGGAGTTCGG AGGAGGGACA GTGACACAAA CGGAAAGCCC CAGGCCCTCT 
CGGGAGAAGG AGAGTGTGGG AAAATCACGC TGGCAGAGCG AGGAAGCGGA AGCGGAAGCG 
GAGGAAGCGC CGAGGGGCAG GGACGGTCGT TCTTTTGAGC TGGAGAGTGA GAGTTTTATA 
GGAAATGGCG CAAGCATAGC TGAAGACGAC GTCGCCCCAG CACAGCGGTC TCCATTAGAA 
CCCGAGTCTC CGGGTTGGCC TGGCTTTGGA GACACCACGA CTGCTAAAGA ATTCAATCAA 
AAATCCCAAG ATGTCGGGTT CTGGGAGGGG GAAGTGGTCC GAGAGCTGTC CGTGGAGGAG 
CAGATAAAGA GAAACCGGTA CTATGACGAG GATGAGGATG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lima1 ( XM_006257355.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257417.1 Rattus norvegicus LIM domain and actin binding 1 (Lima1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa07182
Clone ID Related Accession (Same CDS sequence)	XM_006257355.3 , NM_001191615.2
Accession Version	XM_006257355.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain and actin-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCGA CTCCGTTTAA TAGACGGCAG TGGACTTCCC TGTCACTGAG AGTAACAGCC 
AAAGAACTTT CTCTCGTCAA CAAGAACAAG TCGTCCACAA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAGGAAGA AAAATAACAC TGAAAGTCTC 
CCGCAGCACT TTAGAAGAGG GACCCTGTCT GTGTTAAAGA AGAAGTGGGA GAACCCCGTG 
GCGGGAGCGG AATCCCACAC AGACTCACTG CCAAACAGCA GCAGCGACGG TGGGCACACG 
GCGGACCACC CTCCTGCTGA AGTGACAGCC AAGGCTGCTC CTGGAGCCAG AGCTGACCGG 
GAAGAGCACA CCCAGCCCAG ATCTAGATTT GGATCTCGTC CGGAAGCTGT TACTCAGTGT 
CGGTATCCCC GCTCAGAGGA CAGCCACGAT TTTAAAGCCC AGGCCACGGA AAGCCAAAAT 
ATGGAAAACT GTCTGGGAGA TTCCAGGCAT GAAGCAGAGA AGCCCGAAAT GAGCGAAAAC 
ACGGAAACTT CAGGCAAAAT AGAGAAATAC AACGTTCCAC TGAACAGACT AAAGATGATG 
TTTGAGAAAG GTGAACACAG CCAAAACAAG AGTCCCTGGA CCCAGGGCCG AAATGCAGGC 
GGAAGGAGGC TCTCTGAAAA CAGCTGTTCC CTGGACGATT TGGAAATAGG TGCAGGTCAT 
TTGTCTTCCT CTGCCTTCAA CTCGGAGAAA AATGAGAGTA AGCGGAATCT GGAGCTGCCA 
CGCCTCTCTG AGACCTCCAT AAAGGACCGC ATGGCCAAGT ACCAGGCTGC CGTGTCCAAG 
CAGAGCAGCC CAGCCAGCTA CGCAAGTGAG CTGAAACCCA GTGAAAGCAA AACTCACAAA 
TGGGAGCAGA AGGAGAACGT GCCCCCCGGC CCCGAGGCCT GCAGCATCCA TCAGGAAGGG 
AGCAAGGTTT CTGCAACTGA GAATAGCCTG GTGGCCCACC CTGTCCCTGC AGAAGATGAC 
ACCTGTAACT CCCAGGGGAG GAGTGAGGCC CAGCAGCCCA TATACACTAA GCCGCTGAGT 
CCCGACGCCA GGACCTCCAG TCTTCCGGAA AGTTCTCCTT CCAAAACAGC GAAGAAGTTT 
CAGGCACCCG CAAGAGAGAG CTGCGTGGAG TGTCAGAAGA CGGTGTATCC CATGGAACGG 
CTCCTGGCCA ACCAGCAGGT GTTTCACATC AGCTGTTTCC GATGCTCCTA CTGCAACAAC 
AAACTCAGTC TAGGAACATA TGCATCCTTA CACGGAAGAA TCTATTGTAA GCCTCACTTC 
AACCAACTCT TCAAGTCCAA AGGCAACTAT GACGAAGGCT TTGGGCACAA GCAGCATAAG 
GATCTGTGGG CAAGCAAAGG TGAAAATGAA GAGACTTTGG GGAGACCAGC CCAGCCTCCC 
AGTGCAGGGG AGACCCCCCA TAGCCCGGGT GTAGAAGATG CCCCCATCGC CAAGGTCGGC 
GTGCTGGCTG CGAGTATGGA AGCCAAGGCC TCCTCTCAGA GGGAGAGGGA AGAGAATAAG 
CCAGCGGAGA CCAAGAAGCT GAGGATCGCC TGGCCTCCCC CAGCCGAGCA GGGCAGTTCA 
GGAAGTGCCC CGGAGGAAGG GTTCAAAGTG TCAAAGCCCA AGTGGCCTCC CGAGGATGAA 
GTCTGCAAGA CAGAGGCGCC GGAGGATGTA GATCTGGACC TGAAGAAGCT GAGGCGCTCT 
TCGTCCCTGA AGGAGAGGAG CCGCCCGTTT ACTGTCGCGG CTTCCTTTCG AACCTCCTCG 
GTCAAGAGCC CCAAGCCCTT GTCGCCGTCC CTCAGGAAGG GTTGGAGCGA GCCGGAGCCC 
GAGCAGAGTG AGGAGTTCGG AGGAGGGACA GTGACACAAA CGGAAAGCCC CAGGCCCTCT 
CGGGAGAAGG AGAGTGTGGG AAAATCACGC TGGCAGAGCG AGGAAGCGGA AGCGGAAGCG 
GAGGAAGCGC CGAGGGGCAG GGACGGTCGT TCTTTTGAGC TGGAGAGTGA GAGTTTTATA 
GGAAATGGCG CAAGCATAGC TGAAGACGAC GTCGCCCCAG CACAGCGGTC TCCATTAGAA 
CCCGAGTCTC CGGGTTGGCC TGGCTTTGGA GACACCACGA CTGCTAAAGA ATTCAATCAA 
AAATCCCAAG ATGTCGGGTT CTGGGAGGGG GAAGTGGTCC GAGAGCTGTC CGTGGAGGAG 
CAGATAAAGA GAAACCGGTA CTATGACGAG GATGAGGATG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257417.1
CDS	151..2418
Translation	MESTPFNRRQWTSLSLRVTAKELSLVNKNKSSTIVEIFSKYQKAAEEANMERKKNNTESLPQHFRRGTLSVLKKKWENPVAGAESHTDSLPNSSSDGGHTADHPPAEVTAKAAPGARADREEHTQPRSRFGSRPEAVTQCRYPRSEDSHDFKAQATESQNMENCLGDSRHEAEKPEMSENTETSGKIEKYNVPLNRLKMMFEKGEHSQNKSPWTQGRNAGGRRLSENSCSLDDLEIGAGHLSSSAFNSEKNESKRNLELPRLSETSIKDRMAKYQAAVSKQSSPASYASELKPSESKTHKWEQKENVPPGPEACSIHQEGSKVSATENSLVAHPVPAEDDTCNSQGRSEAQQPIYTKPLSPDARTSSLPESSPSKTAKKFQAPARESCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHKQHKDLWASKGENEETLGRPAQPPSAGETPHSPGVEDAPIAKVGVLAASMEAKASSQREREENKPAETKKLRIAWPPPAEQGSSGSAPEEGFKVSKPKWPPEDEVCKTEAPEDVDLDLKKLRRSSSLKERSRPFTVAASFRTSSVKSPKPLSPSLRKGWSEPEPEQSEEFGGGTVTQTESPRPSREKESVGKSRWQSEEAEAEAEEAPRGRDGRSFELESESFIGNGASIAEDDVAPAQRSPLEPESPGWPGFGDTTTAKEFNQKSQDVGFWEGEVVRELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	XM_006257355.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain and actin binding 1 (Lima1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257355.3

ORF Insert Sequence:

ATGGAATCGA CTCCGTTTAA TAGACGGCAG TGGACTTCCC TGTCACTGAG AGTAACAGCC 
AAAGAACTTT CTCTCGTCAA CAAGAACAAG TCGTCCACAA TTGTGGAAAT ATTCTCCAAG 
TACCAGAAAG CAGCTGAAGA AGCAAACATG GAAAGGAAGA AAAATAACAC TGAAAGTCTC 
CCGCAGCACT TTAGAAGAGG GACCCTGTCT GTGTTAAAGA AGAAGTGGGA GAACCCCGTG 
GCGGGAGCGG AATCCCACAC AGACTCACTG CCAAACAGCA GCAGCGACGG TGGGCACACG 
GCGGACCACC CTCCTGCTGA AGTGACAGCC AAGGCTGCTC CTGGAGCCAG AGCTGACCGG 
GAAGAGCACA CCCAGCCCAG ATCTAGATTT GGATCTCGTC CGGAAGCTGT TACTCAGTGT 
CGGTATCCCC GCTCAGAGGA CAGCCACGAT TTTAAAGCCC AGGCCACGGA AAGCCAAAAT 
ATGGAAAACT GTCTGGGAGA TTCCAGGCAT GAAGCAGAGA AGCCCGAAAT GAGCGAAAAC 
ACGGAAACTT CAGGCAAAAT AGAGAAATAC AACGTTCCAC TGAACAGACT AAAGATGATG 
TTTGAGAAAG GTGAACACAG CCAAAACAAG AGTCCCTGGA CCCAGGGCCG AAATGCAGGC 
GGAAGGAGGC TCTCTGAAAA CAGCTGTTCC CTGGACGATT TGGAAATAGG TGCAGGTCAT 
TTGTCTTCCT CTGCCTTCAA CTCGGAGAAA AATGAGAGTA AGCGGAATCT GGAGCTGCCA 
CGCCTCTCTG AGACCTCCAT AAAGGACCGC ATGGCCAAGT ACCAGGCTGC CGTGTCCAAG 
CAGAGCAGCC CAGCCAGCTA CGCAAGTGAG CTGAAACCCA GTGAAAGCAA AACTCACAAA 
TGGGAGCAGA AGGAGAACGT GCCCCCCGGC CCCGAGGCCT GCAGCATCCA TCAGGAAGGG 
AGCAAGGTTT CTGCAACTGA GAATAGCCTG GTGGCCCACC CTGTCCCTGC AGAAGATGAC 
ACCTGTAACT CCCAGGGGAG GAGTGAGGCC CAGCAGCCCA TATACACTAA GCCGCTGAGT 
CCCGACGCCA GGACCTCCAG TCTTCCGGAA AGTTCTCCTT CCAAAACAGC GAAGAAGTTT 
CAGGCACCCG CAAGAGAGAG CTGCGTGGAG TGTCAGAAGA CGGTGTATCC CATGGAACGG 
CTCCTGGCCA ACCAGCAGGT GTTTCACATC AGCTGTTTCC GATGCTCCTA CTGCAACAAC 
AAACTCAGTC TAGGAACATA TGCATCCTTA CACGGAAGAA TCTATTGTAA GCCTCACTTC 
AACCAACTCT TCAAGTCCAA AGGCAACTAT GACGAAGGCT TTGGGCACAA GCAGCATAAG 
GATCTGTGGG CAAGCAAAGG TGAAAATGAA GAGACTTTGG GGAGACCAGC CCAGCCTCCC 
AGTGCAGGGG AGACCCCCCA TAGCCCGGGT GTAGAAGATG CCCCCATCGC CAAGGTCGGC 
GTGCTGGCTG CGAGTATGGA AGCCAAGGCC TCCTCTCAGA GGGAGAGGGA AGAGAATAAG 
CCAGCGGAGA CCAAGAAGCT GAGGATCGCC TGGCCTCCCC CAGCCGAGCA GGGCAGTTCA 
GGAAGTGCCC CGGAGGAAGG GTTCAAAGTG TCAAAGCCCA AGTGGCCTCC CGAGGATGAA 
GTCTGCAAGA CAGAGGCGCC GGAGGATGTA GATCTGGACC TGAAGAAGCT GAGGCGCTCT 
TCGTCCCTGA AGGAGAGGAG CCGCCCGTTT ACTGTCGCGG CTTCCTTTCG AACCTCCTCG 
GTCAAGAGCC CCAAGCCCTT GTCGCCGTCC CTCAGGAAGG GTTGGAGCGA GCCGGAGCCC 
GAGCAGAGTG AGGAGTTCGG AGGAGGGACA GTGACACAAA CGGAAAGCCC CAGGCCCTCT 
CGGGAGAAGG AGAGTGTGGG AAAATCACGC TGGCAGAGCG AGGAAGCGGA AGCGGAAGCG 
GAGGAAGCGC CGAGGGGCAG GGACGGTCGT TCTTTTGAGC TGGAGAGTGA GAGTTTTATA 
GGAAATGGCG CAAGCATAGC TGAAGACGAC GTCGCCCCAG CACAGCGGTC TCCATTAGAA 
CCCGAGTCTC CGGGTTGGCC TGGCTTTGGA GACACCACGA CTGCTAAAGA ATTCAATCAA 
AAATCCCAAG ATGTCGGGTT CTGGGAGGGG GAAGTGGTCC GAGAGCTGTC CGTGGAGGAG 
CAGATAAAGA GAAACCGGTA CTATGACGAG GATGAGGATG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lima1 ( XM_006257354.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257416.1 Rattus norvegicus LIM domain and actin binding 1 (Lima1), transcript variant X1, mRNA.

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CloneID	ORa40128
Clone ID Related Accession (Same CDS sequence)	XM_006257354.3
Accession Version	XM_006257354.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain and actin-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257354.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGGC AGATGGGATG GCAGCCCACA GTTGTCTCCG AGTCCGTAGA CAAGATGGAA 
TCGACTCCGT TTAATAGACG GCAGTGGACT TCCCTGTCAC TGAGAGTAAC AGCCAAAGAA 
CTTTCTCTCG TCAACAAGAA CAAGTCGTCC ACAATTGTGG AAATATTCTC CAAGTACCAG 
AAAGCAGCTG AAGAAGCAAA CATGGAAAGG AAGAAAAATA ACACTGAAAG TCTCCCGCAG 
CACTTTAGAA GAGGGACCCT GTCTGTGTTA AAGAAGAAGT GGGAGAACCC CGTGGCGGGA 
GCGGAATCCC ACACAGACTC ACTGCCAAAC AGCAGCAGCG ACGGTGGGCA CACGGCGGAC 
CACCCTCCTG CTGAAGTGAC AGCCAAGGCT GCTCCTGGAG CCAGAGCTGA CCGGGAAGAG 
CACACCCAGC CCAGATCTAG ATTTGGATCT CGTCCGGAAG CTGTTACTCA GTGTCGGTAT 
CCCCGCTCAG AGGACAGCCA CGATTTTAAA GCCCAGGCCA CGGAAAGCCA AAATATGGAA 
AACTGTCTGG GAGATTCCAG GCATGAAGCA GAGAAGCCCG AAATGAGCGA AAACACGGAA 
ACTTCAGGCA AAATAGAGAA ATACAACGTT CCACTGAACA GACTAAAGAT GATGTTTGAG 
AAAGGTGAAC ACAGCCAAAA CAAGAGTCCC TGGACCCAGG GCCGAAATGC AGGCGGAAGG 
AGGCTCTCTG AAAACAGCTG TTCCCTGGAC GATTTGGAAA TAGGTGCAGG TCATTTGTCT 
TCCTCTGCCT TCAACTCGGA GAAAAATGAG AGTAAGCGGA ATCTGGAGCT GCCACGCCTC 
TCTGAGACCT CCATAAAGGA CCGCATGGCC AAGTACCAGG CTGCCGTGTC CAAGCAGAGC 
AGCCCAGCCA GCTACGCAAG TGAGCTGAAA CCCAGTGAAA GCAAAACTCA CAAATGGGAG 
CAGAAGGAGA ACGTGCCCCC CGGCCCCGAG GCCTGCAGCA TCCATCAGGA AGGGAGCAAG 
GTTTCTGCAA CTGAGAATAG CCTGGTGGCC CACCCTGTCC CTGCAGAAGA TGACACCTGT 
AACTCCCAGG GGAGGAGTGA GGCCCAGCAG CCCATATACA CTAAGCCGCT GAGTCCCGAC 
GCCAGGACCT CCAGTCTTCC GGAAAGTTCT CCTTCCAAAA CAGCGAAGAA GTTTCAGGCA 
CCCGCAAGAG AGAGCTGCGT GGAGTGTCAG AAGACGGTGT ATCCCATGGA ACGGCTCCTG 
GCCAACCAGC AGGTGTTTCA CATCAGCTGT TTCCGATGCT CCTACTGCAA CAACAAACTC 
AGTCTAGGAA CATATGCATC CTTACACGGA AGAATCTATT GTAAGCCTCA CTTCAACCAA 
CTCTTCAAGT CCAAAGGCAA CTATGACGAA GGCTTTGGGC ACAAGCAGCA TAAGGATCTG 
TGGGCAAGCA AAGGTGAAAA TGAAGAGACT TTGGGGAGAC CAGCCCAGCC TCCCAGTGCA 
GGGGAGACCC CCCATAGCCC GGGTGTAGAA GATGCCCCCA TCGCCAAGGT CGGCGTGCTG 
GCTGCGAGTA TGGAAGCCAA GGCCTCCTCT CAGAGGGAGA GGGAAGAGAA TAAGCCAGCG 
GAGACCAAGA AGCTGAGGAT CGCCTGGCCT CCCCCAGCCG AGCAGGGCAG TTCAGGAAGT 
GCCCCGGAGG AAGGGTTCAA AGTGTCAAAG CCCAAGTGGC CTCCCGAGGA TGAAGTCTGC 
AAGACAGAGG CGCCGGAGGA TGTAGATCTG GACCTGAAGA AGCTGAGGCG CTCTTCGTCC 
CTGAAGGAGA GGAGCCGCCC GTTTACTGTC GCGGCTTCCT TTCGAACCTC CTCGGTCAAG 
AGCCCCAAGC CCTTGTCGCC GTCCCTCAGG AAGGGTTGGA GCGAGCCGGA GCCCGAGCAG 
AGTGAGGAGT TCGGAGGAGG GACAGTGACA CAAACGGAAA GCCCCAGGCC CTCTCGGGAG 
AAGGAGAGTG TGGGAAAATC ACGCTGGCAG AGCGAGGAAG CGGAAGCGGA AGCGGAGGAA 
GCGCCGAGGG GCAGGGACGG TCGTTCTTTT GAGCTGGAGA GTGAGAGTTT TATAGGAAAT 
GGCGCAAGCA TAGCTGAAGA CGACGTCGCC CCAGCACAGC GGTCTCCATT AGAACCCGAG 
TCTCCGGGTT GGCCTGGCTT TGGAGACACC ACGACTGCTA AAGAATTCAA TCAAAAATCC 
CAAGATGTCG GGTTCTGGGA GGGGGAAGTG GTCCGAGAGC TGTCCGTGGA GGAGCAGATA 
AAGAGAAACC GGTACTATGA CGAGGATGAG GATGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257416.1
CDS	411..2732
Translation	MGWQMGWQPTVVSESVDKMESTPFNRRQWTSLSLRVTAKELSLVNKNKSSTIVEIFSKYQKAAEEANMERKKNNTESLPQHFRRGTLSVLKKKWENPVAGAESHTDSLPNSSSDGGHTADHPPAEVTAKAAPGARADREEHTQPRSRFGSRPEAVTQCRYPRSEDSHDFKAQATESQNMENCLGDSRHEAEKPEMSENTETSGKIEKYNVPLNRLKMMFEKGEHSQNKSPWTQGRNAGGRRLSENSCSLDDLEIGAGHLSSSAFNSEKNESKRNLELPRLSETSIKDRMAKYQAAVSKQSSPASYASELKPSESKTHKWEQKENVPPGPEACSIHQEGSKVSATENSLVAHPVPAEDDTCNSQGRSEAQQPIYTKPLSPDARTSSLPESSPSKTAKKFQAPARESCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHKQHKDLWASKGENEETLGRPAQPPSAGETPHSPGVEDAPIAKVGVLAASMEAKASSQREREENKPAETKKLRIAWPPPAEQGSSGSAPEEGFKVSKPKWPPEDEVCKTEAPEDVDLDLKKLRRSSSLKERSRPFTVAASFRTSSVKSPKPLSPSLRKGWSEPEPEQSEEFGGGTVTQTESPRPSREKESVGKSRWQSEEAEAEAEEAPRGRDGRSFELESESFIGNGASIAEDDVAPAQRSPLEPESPGWPGFGDTTTAKEFNQKSQDVGFWEGEVVRELSVEEQIKRNRYYDEDEDEE

Target ORF information:

RefSeq Version	XM_006257354.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM domain and actin binding 1 (Lima1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257354.3

ORF Insert Sequence:

ATGGGATGGC AGATGGGATG GCAGCCCACA GTTGTCTCCG AGTCCGTAGA CAAGATGGAA 
TCGACTCCGT TTAATAGACG GCAGTGGACT TCCCTGTCAC TGAGAGTAAC AGCCAAAGAA 
CTTTCTCTCG TCAACAAGAA CAAGTCGTCC ACAATTGTGG AAATATTCTC CAAGTACCAG 
AAAGCAGCTG AAGAAGCAAA CATGGAAAGG AAGAAAAATA ACACTGAAAG TCTCCCGCAG 
CACTTTAGAA GAGGGACCCT GTCTGTGTTA AAGAAGAAGT GGGAGAACCC CGTGGCGGGA 
GCGGAATCCC ACACAGACTC ACTGCCAAAC AGCAGCAGCG ACGGTGGGCA CACGGCGGAC 
CACCCTCCTG CTGAAGTGAC AGCCAAGGCT GCTCCTGGAG CCAGAGCTGA CCGGGAAGAG 
CACACCCAGC CCAGATCTAG ATTTGGATCT CGTCCGGAAG CTGTTACTCA GTGTCGGTAT 
CCCCGCTCAG AGGACAGCCA CGATTTTAAA GCCCAGGCCA CGGAAAGCCA AAATATGGAA 
AACTGTCTGG GAGATTCCAG GCATGAAGCA GAGAAGCCCG AAATGAGCGA AAACACGGAA 
ACTTCAGGCA AAATAGAGAA ATACAACGTT CCACTGAACA GACTAAAGAT GATGTTTGAG 
AAAGGTGAAC ACAGCCAAAA CAAGAGTCCC TGGACCCAGG GCCGAAATGC AGGCGGAAGG 
AGGCTCTCTG AAAACAGCTG TTCCCTGGAC GATTTGGAAA TAGGTGCAGG TCATTTGTCT 
TCCTCTGCCT TCAACTCGGA GAAAAATGAG AGTAAGCGGA ATCTGGAGCT GCCACGCCTC 
TCTGAGACCT CCATAAAGGA CCGCATGGCC AAGTACCAGG CTGCCGTGTC CAAGCAGAGC 
AGCCCAGCCA GCTACGCAAG TGAGCTGAAA CCCAGTGAAA GCAAAACTCA CAAATGGGAG 
CAGAAGGAGA ACGTGCCCCC CGGCCCCGAG GCCTGCAGCA TCCATCAGGA AGGGAGCAAG 
GTTTCTGCAA CTGAGAATAG CCTGGTGGCC CACCCTGTCC CTGCAGAAGA TGACACCTGT 
AACTCCCAGG GGAGGAGTGA GGCCCAGCAG CCCATATACA CTAAGCCGCT GAGTCCCGAC 
GCCAGGACCT CCAGTCTTCC GGAAAGTTCT CCTTCCAAAA CAGCGAAGAA GTTTCAGGCA 
CCCGCAAGAG AGAGCTGCGT GGAGTGTCAG AAGACGGTGT ATCCCATGGA ACGGCTCCTG 
GCCAACCAGC AGGTGTTTCA CATCAGCTGT TTCCGATGCT CCTACTGCAA CAACAAACTC 
AGTCTAGGAA CATATGCATC CTTACACGGA AGAATCTATT GTAAGCCTCA CTTCAACCAA 
CTCTTCAAGT CCAAAGGCAA CTATGACGAA GGCTTTGGGC ACAAGCAGCA TAAGGATCTG 
TGGGCAAGCA AAGGTGAAAA TGAAGAGACT TTGGGGAGAC CAGCCCAGCC TCCCAGTGCA 
GGGGAGACCC CCCATAGCCC GGGTGTAGAA GATGCCCCCA TCGCCAAGGT CGGCGTGCTG 
GCTGCGAGTA TGGAAGCCAA GGCCTCCTCT CAGAGGGAGA GGGAAGAGAA TAAGCCAGCG 
GAGACCAAGA AGCTGAGGAT CGCCTGGCCT CCCCCAGCCG AGCAGGGCAG TTCAGGAAGT 
GCCCCGGAGG AAGGGTTCAA AGTGTCAAAG CCCAAGTGGC CTCCCGAGGA TGAAGTCTGC 
AAGACAGAGG CGCCGGAGGA TGTAGATCTG GACCTGAAGA AGCTGAGGCG CTCTTCGTCC 
CTGAAGGAGA GGAGCCGCCC GTTTACTGTC GCGGCTTCCT TTCGAACCTC CTCGGTCAAG 
AGCCCCAAGC CCTTGTCGCC GTCCCTCAGG AAGGGTTGGA GCGAGCCGGA GCCCGAGCAG 
AGTGAGGAGT TCGGAGGAGG GACAGTGACA CAAACGGAAA GCCCCAGGCC CTCTCGGGAG 
AAGGAGAGTG TGGGAAAATC ACGCTGGCAG AGCGAGGAAG CGGAAGCGGA AGCGGAGGAA 
GCGCCGAGGG GCAGGGACGG TCGTTCTTTT GAGCTGGAGA GTGAGAGTTT TATAGGAAAT 
GGCGCAAGCA TAGCTGAAGA CGACGTCGCC CCAGCACAGC GGTCTCCATT AGAACCCGAG 
TCTCCGGGTT GGCCTGGCTT TGGAGACACC ACGACTGCTA AAGAATTCAA TCAAAAATCC 
CAAGATGTCG GGTTCTGGGA GGGGGAAGTG GTCCGAGAGC TGTCCGTGGA GGAGCAGATA 
AAGAGAAACC GGTACTATGA CGAGGATGAG GATGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A
Science (New York, N.Y.)360(6393)1087-1092(2018 06)
Zhang YY,Fu ZY,Wei J,Qi W,Baituola G,Luo J,Meng YJ,Guo SY,Yin H,Jiang SY,Li YF,Miao HH,Liu Y,Wang Y,Li BL,Ma YT,Song BL

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Epithelial protein lost in neoplasm modulates platelet-derived growth factor-mediated adhesion and motility of mesangial cells.
Kidney international86(3)548-57(2014 Sep)
Tsurumi H,Harita Y,Kurihara H,Kosako H,Hayashi K,Matsunaga A,Kajiho Y,Kanda S,Miura K,Sekine T,Oka A,Ishizuka K,Horita S,Hattori M,Hattori S,Igarashi T

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Molecular biology of the cell23(2)324-36(2012 Jan)
Revenu C,Ubelmann F,Hurbain I,El-Marjou F,Dingli F,Loew D,Delacour D,Gilet J,Brot-Laroche E,Rivero F,Louvard D,Robine S

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

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Lima1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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