Mars cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mars
Entrez Gene ID	299851
Full Name	methionyl-tRNA synthetase
General protein information	Preferred Names methionyl-tRNA synthetase Names methionine--tRNA ligase, cytoplasmic methionine-tRNA synthetase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127659.1	NP_001121131.1	methionine--tRNA ligase, cytoplasmic
XM_006241451.3	XP_006241513.1	methionine--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A09647g	XP_451421.1
Schizosaccharomyces pombe (fission yeast)	rar1	NP_595586.1
Bos taurus (cattle)	MARS	NP_001033180.1
Mus musculus (house mouse)	Mars	NP_001165053.1
Rattus norvegicus (Norway rat)	Mars	NP_001121131.1
Eremothecium gossypii	AGOS_AGR332C	NP_986998.1
Homo sapiens (human)	MARS	NP_004981.2
Pan troglodytes (chimpanzee)	MARS	XP_509164.2
Macaca mulatta (Rhesus monkey)	MARS	XP_001116063.1
Canis lupus familiaris (dog)	MARS	XP_531646.2
Gallus gallus (chicken)	MARS	NP_001244301.1
Danio rerio (zebrafish)	mars	NP_956370.1
Xenopus tropicalis (tropical clawed frog)	mars	NP_001017000.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007891	XP_317601.4
Neurospora crassa	NCU07451	XP_964638.1
Drosophila melanogaster (fruit fly)	CG15100	NP_611382.1
Oryza sativa (rice)	Os10g0400100	NP_001064541.1
Magnaporthe oryzae (rice blast fungus)	MGG_01046	XP_003717896.1
Saccharomyces cerevisiae (baker's yeast)	MES1	NP_011780.3

Gene Ontology
Bibliography

Related articles in PubMed

The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.
Topalidou I, Cattin-Ortolá J, Pappas AL, Cooper K, Merrihew GE, MacCoss MJ, Ailion M
PLoS genetics12(5)e1006074(2016 05)

Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway.
Han JM, Jeong SJ, Park MC, Kim G, Kwon NH, Kim HK, Ha SH, Ryu SH, Kim S
Cell149(2)410-24(2012 Apr)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

LysRS serves as a key signaling molecule in the immune response by regulating gene expression.
Yannay-Cohen N, Carmi-Levy I, Kay G, Yang CM, Han JM, Kemeny DM, Kim S, Nechushtan H, Razin E
Molecular cell34(5)603-11(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02083	NM_001127659.1 Latest version!	Rattus norvegicus methionyl-tRNA synthetase 1 (Mars1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa30242	XM_006241451.3 Latest version!	Rattus norvegicus methionyl-tRNA synthetase (Mars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mars ( NM_001127659.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001121131.1 Rattus norvegicus methionyl-tRNA synthetase 1 (Mars1), mRNA.

Price & Availability↑

CloneID	ORa02083
Clone ID Related Accession (Same CDS sequence)	NM_001127659.1
Accession Version	NM_001127659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565539200000
Organism	Rattus norvegicus(Norway rat)
Product	methionine--tRNA ligase, cytoplasmic
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114111.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGGCTGT TCGTGAGCGA AGGCTCCCCG GGTAGCCTGC CGGTGCTGGC TGCGGCCGCG 
AGGGCCCGGG GCCGGGCGGA GCTGCTCATC AGCACCGTAG GCCCCGAAGA GTGTGTGGTA 
CCATTTCTTA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGCGGCAA CTACCTCTTC 
TCTGCTAGTG CAATCTGCCG ATATTTTTTT CTGTTATGCG GCTGGGAACA AGATGATCTT 
ACCAACCAAT GGCTGGAATG GGAAGCAACA GAACTGCAGC CGGTTCTGTC TGCTGCCCTA 
TACTGTCTAG TGGTTCAAGG CAAAAAAGGG GAAGATGTAC TTGGCCCACT TCGGAGAGTC 
CTGACTCACA TTGATCACAG CTTGAGTCGG CAAAACTGTC CTTTCTTGGC AGGGGACACA 
GAATCTCTAG CTGACATTGT TTTGTGGGGA GCACTATATC CTTTACTGCA AGACCCAGCT 
TACCTCCCTG AGGAGTTGGG TGCCCTACAA AGCTGGTTCC AGACACTAAG TACCCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAACAGCAGG GTGTCCTGGC ACTGCGTCCC 
TACCTCCAAA AACAGCCCCA GCCCCAGCCC CTACCTCCTG AGGGGAGAGC TGTCAGCAAT 
GAACCTGAGG AGGAAGAGCT GGCTACCCTG TCTGAGGAGG ACATCATTGC TGCTGTTGCT 
GCTTGGGAGA AGGGTCTGGA AAACTTACCT CCACTACAGC CCCAGCAGCA TCCAGTGTTG 
CCTGTGCCTG GAGAAAGGAA TGTGCTCATC ACGAGTGCCC TCCCTTATGT CAACAATGTC 
CCCCACCTTG GAAACATCAT TGGCTGTGTG CTCAGTGCTG ATGTCTTTGC AAGGTACTCT 
CGCCTTCGCC AGTGGAACAC CCTCTATCTG TGTGGCACAG ATGAGTATGG TACTGCGACA 
GAGACCAAGG CCATGGAAGA GGGTCTAACC CCACAAGAAA TCTGTGACAA ATACCACGCC 
ATACATGTTG ACATCTACCG CTGGTTCAAT ATATCGTTTG ATACGTTCGG TCGCACAACC 
ACTCCTCTGC AGACCAAAAT CACACAGGAC ATCTTCCAGA GGTTGCTGAC ACGGGGTTTT 
GTGCTGCAAG ATACTGTAGA GCAGCTTCGC TGTGAGCAGT GTGCACGTTT CTTGGCTGAC 
CGCTTTGTGG AGGGAGTGTG TCCCTTTTGT GGCTATGAAG AGGCCCGGGG TGACCAGTGT 
GACAAGTGTG GCAAGCTCAT CAATGCCATT GAGCTCAAGA GACCTCAGTG CAAAGTCTGC 
CGCTCCCACC CTGTGGTGAA GTCCTCAAAG CACCTGTTTC TAGACTTGCC TAAGTTAGAG 
AAGCGCCTGG AGGACTGGCT GGGGAAGACG ATGCCTGGCA GTGACTGGAC ACCCAATGCC 
AGGTTCATTA TTCGATCCTG GCTTCGAGAT GGACTCAAGC CACGATGCAT CACCAGAGAC 
CTCAAATGGG GAACTCCTGT GCCCTTGGAA GGTTTTGAGG ACAAGGTATT TTATGTCTGG 
TTTGATGCTA CTATTGGCTA TCTGTCCATC ACAGCCAACT ACACAGACCA GTGGGAGAGA 
TGGTGGAAGA ATCCAGAACA AGTGGACCTT TACCAGTTCA TGGCCAAAGA CAATGTTCCC 
TTCCATGGCT TGGTCTTTCC TTGTTCAGCC CTAGGAGCTG AGGATAACTA CACCTTAGTC 
AAGAACCTCA TCGCTACAGA ATACCTGAAC TACGAGGACG GGAAGTTCTC TAAGAGCCGG 
GGGATAGGAG TGTTTGGAGA CATGGCCCAA GATACAGGGA TCCCTGCTGA CATCTGGCGA 
TTCTATCTGC TATACATTCG GCCTGAGGGC CAGGACAGTG CCTTCTCCTG GACAGACTTG 
CTGATTAAAA ACAATTCTGA GCTGCTTAAC AACCTGGGCA ACTTCATCAA TAGAGCTGGC 
ATGTTCGTGT CTAAGTTTTT TGGGGGTTGT GTGCCTGAGA TGGTGCTGAC CCACGATGAC 
AGACGCCTGG TGGCACATGT CTCCTGGGAA CTCCAGCGCT ATCACCAGCT GTTGGAAAAG 
GTTCGGATCC GGGATGCCTT GCGCAGTATC CTTACCATAT CTCGGCATGG CAACCAATAT 
ATTCAAGTGA ATGAGCCCTG GAAACGGATT AAAGGCGATG AGATGGACAG GCAGCGGGCA 
GGCACGGTGA CAGGAATGGC AGTGAACATA GCTGCCTTGC TGTCTGTCAT GCTCCAGCCG 
TACATGCCCA CAGTCAGCTC CACCATTCAG ACCCAGCTCC AGCTCCCAGA GGCAGCCTGC 
CGCATCCTTG CCACAAGCTT CATTTGTACC TTACCAGCAG GCCACCGAAT TGGCACAGTC 
AGTCCTTTGT TCCAGAAATT GGAAAATGAC CAGATTGAAA ATTTGAGGCA GCGCTTTGGA 
GGGGGCCAGG CTAAAGGGTC CCCCAAGCCA GCAGTTGTTG AGGCAGTTAC TACAGCAGGG 
TCGCAGGACA TACAAGTGCT GGTGGATGAG GTAACCAAAC AGGGAAACAT AGTTCGGGAA 
CTGAAAGCAC AGAAGGCAGA CAAGAACCAG GTTGCTGCAG AAGTGGCTAA ACTCTTGGAT 
CTAAAGAAAC AGTTGGCTTT GGCTGAGGGG AAGCCCATTG AAACTCCGAA AGGCAAGAAG 
AAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121131.1
CDS	11..2719
Translation	MRLFVSEGSPGSLPVLAAAARARGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSASAICRYFFLLCGWEQDDLTNQWLEWEATELQPVLSAALYCLVVQGKKGEDVLGPLRRVLTHIDHSLSRQNCPFLAGDTESLADIVLWGALYPLLQDPAYLPEELGALQSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPQPLPPEGRAVSNEPEEEELATLSEEDIIAAVAAWEKGLENLPPLQPQQHPVLPVPGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKAMEEGLTPQEICDKYHAIHVDIYRWFNISFDTFGRTTTPLQTKITQDIFQRLLTRGFVLQDTVEQLRCEQCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAIELKRPQCKVCRSHPVVKSSKHLFLDLPKLEKRLEDWLGKTMPGSDWTPNARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHGLVFPCSALGAEDNYTLVKNLIATEYLNYEDGKFSKSRGIGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSKFFGGCVPEMVLTHDDRRLVAHVSWELQRYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGDEMDRQRAGTVTGMAVNIAALLSVMLQPYMPTVSSTIQTQLQLPEAACRILATSFICTLPAGHRIGTVSPLFQKLENDQIENLRQRFGGGQAKGSPKPAVVEAVTTAGSQDIQVLVDEVTKQGNIVRELKAQKADKNQVAAEVAKLLDLKKQLALAEGKPIETPKGKKKK

Target ORF information:

RefSeq Version	NM_001127659.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methionyl-tRNA synthetase 1 (Mars1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127659.1

ORF Insert Sequence:

ATGAGGCTGT TCGTGAGCGA AGGCTCCCCG GGTAGCCTGC CGGTGCTGGC TGCGGCCGCG 
AGGGCCCGGG GCCGGGCGGA GCTGCTCATC AGCACCGTAG GCCCCGAAGA GTGTGTGGTA 
CCATTTCTTA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGCGGCAA CTACCTCTTC 
TCTGCTAGTG CAATCTGCCG ATATTTTTTT CTGTTATGCG GCTGGGAACA AGATGATCTT 
ACCAACCAAT GGCTGGAATG GGAAGCAACA GAACTGCAGC CGGTTCTGTC TGCTGCCCTA 
TACTGTCTAG TGGTTCAAGG CAAAAAAGGG GAAGATGTAC TTGGCCCACT TCGGAGAGTC 
CTGACTCACA TTGATCACAG CTTGAGTCGG CAAAACTGTC CTTTCTTGGC AGGGGACACA 
GAATCTCTAG CTGACATTGT TTTGTGGGGA GCACTATATC CTTTACTGCA AGACCCAGCT 
TACCTCCCTG AGGAGTTGGG TGCCCTACAA AGCTGGTTCC AGACACTAAG TACCCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAACAGCAGG GTGTCCTGGC ACTGCGTCCC 
TACCTCCAAA AACAGCCCCA GCCCCAGCCC CTACCTCCTG AGGGGAGAGC TGTCAGCAAT 
GAACCTGAGG AGGAAGAGCT GGCTACCCTG TCTGAGGAGG ACATCATTGC TGCTGTTGCT 
GCTTGGGAGA AGGGTCTGGA AAACTTACCT CCACTACAGC CCCAGCAGCA TCCAGTGTTG 
CCTGTGCCTG GAGAAAGGAA TGTGCTCATC ACGAGTGCCC TCCCTTATGT CAACAATGTC 
CCCCACCTTG GAAACATCAT TGGCTGTGTG CTCAGTGCTG ATGTCTTTGC AAGGTACTCT 
CGCCTTCGCC AGTGGAACAC CCTCTATCTG TGTGGCACAG ATGAGTATGG TACTGCGACA 
GAGACCAAGG CCATGGAAGA GGGTCTAACC CCACAAGAAA TCTGTGACAA ATACCACGCC 
ATACATGTTG ACATCTACCG CTGGTTCAAT ATATCGTTTG ATACGTTCGG TCGCACAACC 
ACTCCTCTGC AGACCAAAAT CACACAGGAC ATCTTCCAGA GGTTGCTGAC ACGGGGTTTT 
GTGCTGCAAG ATACTGTAGA GCAGCTTCGC TGTGAGCAGT GTGCACGTTT CTTGGCTGAC 
CGCTTTGTGG AGGGAGTGTG TCCCTTTTGT GGCTATGAAG AGGCCCGGGG TGACCAGTGT 
GACAAGTGTG GCAAGCTCAT CAATGCCATT GAGCTCAAGA GACCTCAGTG CAAAGTCTGC 
CGCTCCCACC CTGTGGTGAA GTCCTCAAAG CACCTGTTTC TAGACTTGCC TAAGTTAGAG 
AAGCGCCTGG AGGACTGGCT GGGGAAGACG ATGCCTGGCA GTGACTGGAC ACCCAATGCC 
AGGTTCATTA TTCGATCCTG GCTTCGAGAT GGACTCAAGC CACGATGCAT CACCAGAGAC 
CTCAAATGGG GAACTCCTGT GCCCTTGGAA GGTTTTGAGG ACAAGGTATT TTATGTCTGG 
TTTGATGCTA CTATTGGCTA TCTGTCCATC ACAGCCAACT ACACAGACCA GTGGGAGAGA 
TGGTGGAAGA ATCCAGAACA AGTGGACCTT TACCAGTTCA TGGCCAAAGA CAATGTTCCC 
TTCCATGGCT TGGTCTTTCC TTGTTCAGCC CTAGGAGCTG AGGATAACTA CACCTTAGTC 
AAGAACCTCA TCGCTACAGA ATACCTGAAC TACGAGGACG GGAAGTTCTC TAAGAGCCGG 
GGGATAGGAG TGTTTGGAGA CATGGCCCAA GATACAGGGA TCCCTGCTGA CATCTGGCGA 
TTCTATCTGC TATACATTCG GCCTGAGGGC CAGGACAGTG CCTTCTCCTG GACAGACTTG 
CTGATTAAAA ACAATTCTGA GCTGCTTAAC AACCTGGGCA ACTTCATCAA TAGAGCTGGC 
ATGTTCGTGT CTAAGTTTTT TGGGGGTTGT GTGCCTGAGA TGGTGCTGAC CCACGATGAC 
AGACGCCTGG TGGCACATGT CTCCTGGGAA CTCCAGCGCT ATCACCAGCT GTTGGAAAAG 
GTTCGGATCC GGGATGCCTT GCGCAGTATC CTTACCATAT CTCGGCATGG CAACCAATAT 
ATTCAAGTGA ATGAGCCCTG GAAACGGATT AAAGGCGATG AGATGGACAG GCAGCGGGCA 
GGCACGGTGA CAGGAATGGC AGTGAACATA GCTGCCTTGC TGTCTGTCAT GCTCCAGCCG 
TACATGCCCA CAGTCAGCTC CACCATTCAG ACCCAGCTCC AGCTCCCAGA GGCAGCCTGC 
CGCATCCTTG CCACAAGCTT CATTTGTACC TTACCAGCAG GCCACCGAAT TGGCACAGTC 
AGTCCTTTGT TCCAGAAATT GGAAAATGAC CAGATTGAAA ATTTGAGGCA GCGCTTTGGA 
GGGGGCCAGG CTAAAGGGTC CCCCAAGCCA GCAGTTGTTG AGGCAGTTAC TACAGCAGGG 
TCGCAGGACA TACAAGTGCT GGTGGATGAG GTAACCAAAC AGGGAAACAT AGTTCGGGAA 
CTGAAAGCAC AGAAGGCAGA CAAGAACCAG GTTGCTGCAG AAGTGGCTAA ACTCTTGGAT 
CTAAAGAAAC AGTTGGCTTT GGCTGAGGGG AAGCCCATTG AAACTCCGAA AGGCAAGAAG 
AAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mars ( XM_006241451.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241513.1 Rattus norvegicus methionyl-tRNA synthetase (Mars), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30242
Clone ID Related Accession (Same CDS sequence)	XM_006241451.3
Accession Version	XM_006241451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	methionine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGT TCGTGAGCGA AGGCTCCCCG GGTAGCCTGC CGGTGCTGGC TGCGGCCGCG 
AGGGCCCGGG GCCGGGCGGA GCTGCTCATC AGCACCGTAG GCCCCGAAGA GTGTGTGGTA 
CCATTTCTTA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGCGGCAA CTACCTCTTC 
TCTGCTAGTG CAATCTGCCG ATATTTTTTT CTGTTATGCG GCTGGGAACA AGATGATCTT 
ACCAACCAAT GGCTGGAATG GGAAGCAACA GAACTGCAGC CGGTTCTGTC TGCTGCCCTA 
TACTGTCTAG TGGTTCAAGG CAAAAAAGGG GAAGATGTAC TTGGCCCACT TCGGAGAGTC 
CTGACTCACA TTGATCACAG CTTGAGTCGG CAAAACTGTC CTTTCTTGGC AGGGGACACA 
GAATCTCTAG CTGACATTGT TTTGTGGGGA GCACTATATC CTTTACTGCA AGACCCAGCT 
TACCTCCCTG AGGAGTTGGG TGCCCTACAA AGCTGGTTCC AGACACTAAG TACCCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAACAGCAGG GTGTCCTGGC ACTGCGTCCC 
TACCTCCAAA AACAGCCCCA GCCCCAGCCC CTACCTCCTG AGGGGAGAGC TGTCAGCAAT 
GAACCTGAGG AGGAAGAGCT GGCTACCCTG TCTGAGGAGG ACATCATTGC TGCTGTTGCT 
GCTTGGGAGA AGGGTCTGGA AAACTTACCT CCACTACAGC CCCAGCAGCA TCCAGTGTTG 
CCTGTGCCTG GAGAAAGGAA TGTGCTCATC ACGAGTGCCC TCCCTTATGT CAACAATGTC 
CCCCACCTTG GAAACATCAT TGGCTGTGTG CTCAGTGCTG ATGTCTTTGC AAGGTACTCT 
CGCCTTCGCC AGTGGAACAC CCTCTATCTG TGTGGCACAG ATGAGTATGG TACTGCGACA 
GAGACCAAGG CCATGGAAGA GGGTCTAACC CCACAAGAAA TCTGTGACAA ATACCACGCC 
ATACATGTTG ACATCTACCG CTGGTTCAAT ATATCGTTTG ATACGTTCGG TCGCACAACC 
ACTCCTCTGC AGACCAAAAT CACACAGGAC ATCTTCCAGA GGTTGCTGAC ACGGGGTTTT 
GTGCTGCAAG ATACTGTAGA GCAGCTTCGC TGTGAGCAGT GTGCACGTTT CTTGGCTGAC 
CGCTTTGTGG AGGGAGTGTG TCCCTTTTGT GGCTATGAAG AGGCCCGGGG TGACCAGTGT 
GACAAGTGTG GCAAGCTCAT CAATGCCATT GAGCTCAAGA GACCTCAGTG CAAAGTCTGC 
CGCTCCCACC CTGTGGTGAA GTCCTCAAAG CACCTGTTTC TAGACTTGCC TAAGTTAGAG 
AAGCGCCTGG AGGACTGGCT GGGGAAGACG ATGCCTGGCA GTGACTGGAC ACCCAATGCC 
AGGTTCATTA TTCGATCCTG GCTTCGAGAT GGACTCAAGC CACGATGCAT CACCAGAGAC 
CTCAAATGGG GAACTCCTGT GCCCTTGGAA GGTTTTGAGG ACAAGGTATT TTATGTCTGG 
TTTGATGCTA CTATTGGCTA TCTGTCCATC ACAGCCAACT ACACAGACCA GTGGGAGAGA 
TGGTGGAAGA ATCCAGAACA AGTGGACCTT TACCAGTTCA TGGCCAAAGA CAATGTTCCC 
TTCCATGGCT TGGTCTTTCC TTGTTCAGCC CTAGGAGCTG AGGATAACTA CACCTTAGTC 
AAGAACCTCA TCGCTACAGA ATACCTGAAC TACGAGGACG GGAAGTTCTC TAAGAGCCGG 
GGGATAGGAG TGTTTGGAGA CATGGCCCAA GATACAGGGA TCCCTGCTGA CATCTGGCGA 
TTCTATCTGC TATACATTCG GCCTGAGGGC CAGGACAGTG CCTTCTCCTG GACAGACTTG 
CTGATTAAAA ACAATTCTGA GCTGCTTAAC AACCTGGGCA ACTTCATCAA TAGAGCTGGC 
ATGTTCGTGT CTAAGTTTTT TGGGGGTTGT GTGCCTGAGA TGGTGCTGAC CCACGATGAC 
AGACGCCTGG TGGCACATGT CTCCTGGGAA CTCCAGCGCT ATCACCAGCT GTTGGAAAAG 
GTTCGGATCC GGGATGCCTT GCGCAGTATC CTTACCATAT CTCGGCATGG CAACCAATAT 
ATTCAAGTGA ATGAGCCCTG GAAACGGATT AAAGGCGATG AGATGGACAG GCAGCGGGCA 
GGCACGGTGA CAGGAATGGC AGTGAACATA GCTGCCTTGC TGTCTGTCAT GCTCCAGCCG 
TACATGCCCA CAGTCAGCTC CACCATTCAG ACCCAGCTCC AGCTCCCAGA GGCAGCCTGC 
CGCATCCTTG CCACAAGCTT CATTTGTACC TTACCAGCAG GCCACCGAAT TGGCACAGTC 
AGTCCTTTGT TCCAGAAATT GGAAAATGAC CAGATTGAAA ATTTGAGGCA GCGCTTTGGA 
GGGGGCCAGC TAGAAGAGTC TTTGGAGTTA CAGGCTAAAG GGTCCCCCAA GCCAGCAGTT 
GTTGAGGCAG TTACTACAGC AGGGTCGCAG GACATACAAG TGCTGGTGGA TGAGGTAACC 
AAACAGGGAA ACATAGTTCG GGAACTGAAA GCACAGAAGG CAGACAAGAA CCAGGTTGCT 
GCAGAAGTGG CTAAACTCTT GGATCTAAAG AAACAGTTGG CTTTGGCTGA GGGGAAGCCC 
ATTGAAACTC CGAAAGGCAA GAAGAAAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241513.1
CDS	126..2858
Translation	MRLFVSEGSPGSLPVLAAAARARGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSASAICRYFFLLCGWEQDDLTNQWLEWEATELQPVLSAALYCLVVQGKKGEDVLGPLRRVLTHIDHSLSRQNCPFLAGDTESLADIVLWGALYPLLQDPAYLPEELGALQSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPQPLPPEGRAVSNEPEEEELATLSEEDIIAAVAAWEKGLENLPPLQPQQHPVLPVPGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKAMEEGLTPQEICDKYHAIHVDIYRWFNISFDTFGRTTTPLQTKITQDIFQRLLTRGFVLQDTVEQLRCEQCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAIELKRPQCKVCRSHPVVKSSKHLFLDLPKLEKRLEDWLGKTMPGSDWTPNARFIIRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHGLVFPCSALGAEDNYTLVKNLIATEYLNYEDGKFSKSRGIGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSKFFGGCVPEMVLTHDDRRLVAHVSWELQRYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGDEMDRQRAGTVTGMAVNIAALLSVMLQPYMPTVSSTIQTQLQLPEAACRILATSFICTLPAGHRIGTVSPLFQKLENDQIENLRQRFGGGQLEESLELQAKGSPKPAVVEAVTTAGSQDIQVLVDEVTKQGNIVRELKAQKADKNQVAAEVAKLLDLKKQLALAEGKPIETPKGKKKK

Target ORF information:

RefSeq Version	XM_006241451.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methionyl-tRNA synthetase (Mars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241451.3

ORF Insert Sequence:

ATGAGGCTGT TCGTGAGCGA AGGCTCCCCG GGTAGCCTGC CGGTGCTGGC TGCGGCCGCG 
AGGGCCCGGG GCCGGGCGGA GCTGCTCATC AGCACCGTAG GCCCCGAAGA GTGTGTGGTA 
CCATTTCTTA CCCGGCCTAA GGTCCCTGTC TTGCAGCTGG ATAGCGGCAA CTACCTCTTC 
TCTGCTAGTG CAATCTGCCG ATATTTTTTT CTGTTATGCG GCTGGGAACA AGATGATCTT 
ACCAACCAAT GGCTGGAATG GGAAGCAACA GAACTGCAGC CGGTTCTGTC TGCTGCCCTA 
TACTGTCTAG TGGTTCAAGG CAAAAAAGGG GAAGATGTAC TTGGCCCACT TCGGAGAGTC 
CTGACTCACA TTGATCACAG CTTGAGTCGG CAAAACTGTC CTTTCTTGGC AGGGGACACA 
GAATCTCTAG CTGACATTGT TTTGTGGGGA GCACTATATC CTTTACTGCA AGACCCAGCT 
TACCTCCCTG AGGAGTTGGG TGCCCTACAA AGCTGGTTCC AGACACTAAG TACCCAGGAG 
CCATGTCAGC GAGCTGCAGA GACTGTGCTG AAACAGCAGG GTGTCCTGGC ACTGCGTCCC 
TACCTCCAAA AACAGCCCCA GCCCCAGCCC CTACCTCCTG AGGGGAGAGC TGTCAGCAAT 
GAACCTGAGG AGGAAGAGCT GGCTACCCTG TCTGAGGAGG ACATCATTGC TGCTGTTGCT 
GCTTGGGAGA AGGGTCTGGA AAACTTACCT CCACTACAGC CCCAGCAGCA TCCAGTGTTG 
CCTGTGCCTG GAGAAAGGAA TGTGCTCATC ACGAGTGCCC TCCCTTATGT CAACAATGTC 
CCCCACCTTG GAAACATCAT TGGCTGTGTG CTCAGTGCTG ATGTCTTTGC AAGGTACTCT 
CGCCTTCGCC AGTGGAACAC CCTCTATCTG TGTGGCACAG ATGAGTATGG TACTGCGACA 
GAGACCAAGG CCATGGAAGA GGGTCTAACC CCACAAGAAA TCTGTGACAA ATACCACGCC 
ATACATGTTG ACATCTACCG CTGGTTCAAT ATATCGTTTG ATACGTTCGG TCGCACAACC 
ACTCCTCTGC AGACCAAAAT CACACAGGAC ATCTTCCAGA GGTTGCTGAC ACGGGGTTTT 
GTGCTGCAAG ATACTGTAGA GCAGCTTCGC TGTGAGCAGT GTGCACGTTT CTTGGCTGAC 
CGCTTTGTGG AGGGAGTGTG TCCCTTTTGT GGCTATGAAG AGGCCCGGGG TGACCAGTGT 
GACAAGTGTG GCAAGCTCAT CAATGCCATT GAGCTCAAGA GACCTCAGTG CAAAGTCTGC 
CGCTCCCACC CTGTGGTGAA GTCCTCAAAG CACCTGTTTC TAGACTTGCC TAAGTTAGAG 
AAGCGCCTGG AGGACTGGCT GGGGAAGACG ATGCCTGGCA GTGACTGGAC ACCCAATGCC 
AGGTTCATTA TTCGATCCTG GCTTCGAGAT GGACTCAAGC CACGATGCAT CACCAGAGAC 
CTCAAATGGG GAACTCCTGT GCCCTTGGAA GGTTTTGAGG ACAAGGTATT TTATGTCTGG 
TTTGATGCTA CTATTGGCTA TCTGTCCATC ACAGCCAACT ACACAGACCA GTGGGAGAGA 
TGGTGGAAGA ATCCAGAACA AGTGGACCTT TACCAGTTCA TGGCCAAAGA CAATGTTCCC 
TTCCATGGCT TGGTCTTTCC TTGTTCAGCC CTAGGAGCTG AGGATAACTA CACCTTAGTC 
AAGAACCTCA TCGCTACAGA ATACCTGAAC TACGAGGACG GGAAGTTCTC TAAGAGCCGG 
GGGATAGGAG TGTTTGGAGA CATGGCCCAA GATACAGGGA TCCCTGCTGA CATCTGGCGA 
TTCTATCTGC TATACATTCG GCCTGAGGGC CAGGACAGTG CCTTCTCCTG GACAGACTTG 
CTGATTAAAA ACAATTCTGA GCTGCTTAAC AACCTGGGCA ACTTCATCAA TAGAGCTGGC 
ATGTTCGTGT CTAAGTTTTT TGGGGGTTGT GTGCCTGAGA TGGTGCTGAC CCACGATGAC 
AGACGCCTGG TGGCACATGT CTCCTGGGAA CTCCAGCGCT ATCACCAGCT GTTGGAAAAG 
GTTCGGATCC GGGATGCCTT GCGCAGTATC CTTACCATAT CTCGGCATGG CAACCAATAT 
ATTCAAGTGA ATGAGCCCTG GAAACGGATT AAAGGCGATG AGATGGACAG GCAGCGGGCA 
GGCACGGTGA CAGGAATGGC AGTGAACATA GCTGCCTTGC TGTCTGTCAT GCTCCAGCCG 
TACATGCCCA CAGTCAGCTC CACCATTCAG ACCCAGCTCC AGCTCCCAGA GGCAGCCTGC 
CGCATCCTTG CCACAAGCTT CATTTGTACC TTACCAGCAG GCCACCGAAT TGGCACAGTC 
AGTCCTTTGT TCCAGAAATT GGAAAATGAC CAGATTGAAA ATTTGAGGCA GCGCTTTGGA 
GGGGGCCAGC TAGAAGAGTC TTTGGAGTTA CAGGCTAAAG GGTCCCCCAA GCCAGCAGTT 
GTTGAGGCAG TTACTACAGC AGGGTCGCAG GACATACAAG TGCTGGTGGA TGAGGTAACC 
AAACAGGGAA ACATAGTTCG GGAACTGAAA GCACAGAAGG CAGACAAGAA CCAGGTTGCT 
GCAGAAGTGG CTAAACTCTT GGATCTAAAG AAACAGTTGG CTTTGGCTGA GGGGAAGCCC 
ATTGAAACTC CGAAAGGCAA GAAGAAAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.
PLoS genetics12(5)e1006074(2016 05)
Topalidou I,Cattin-Ortolá J,Pappas AL,Cooper K,Merrihew GE,MacCoss MJ,Ailion M

Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway.
Cell149(2)410-24(2012 Apr)
Han JM,Jeong SJ,Park MC,Kim G,Kwon NH,Kim HK,Ha SH,Ryu SH,Kim S

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

LysRS serves as a key signaling molecule in the immune response by regulating gene expression.
Molecular cell34(5)603-11(2009 Jun)
Yannay-Cohen N,Carmi-Levy I,Kay G,Yang CM,Han JM,Kemeny DM,Kim S,Nechushtan H,Razin E

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Mars cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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