Rhoj cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhoj
Entrez Gene ID	299145
Full Name	ras homolog family member J
General protein information	Preferred Names ras homolog family member J Names rho-related GTP-binding protein RhoJ TC10-like Rho GTPase ras homolog gene family, member J
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008320.1	NP_001008321.1	rho-related GTP-binding protein RhoJ

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RHOJ	NP_001264477.1
Pan troglodytes (chimpanzee)	RHOJ	XP_509996.2
Bos taurus (cattle)	RHOJ	NP_001076967.1
Rattus norvegicus (Norway rat)	Rhoj	NP_001008321.1
Danio rerio (zebrafish)	rhoj	NP_001038266.1
Xenopus tropicalis (tropical clawed frog)	rhoj	XP_002939520.1
Macaca mulatta (Rhesus monkey)	RHOJ	XP_001100323.1
Mus musculus (house mouse)	Rhoj	NP_075764.1
Homo sapiens (human)	RHOJ	NP_065714.1
Canis lupus familiaris (dog)	RHOJ	XP_854125.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of glutamine synthetase in angiogenesis beyond glutamine synthesis.
Eelen G, Dubois C, Cantelmo AR, Goveia J, Brüning U, DeRan M, Jarugumilli G, van Rijssel J, Saladino G, Comitani F, Zecchin A, Rocha S, Chen R, Huang H, Vandekeere S, Kalucka J, Lange C, Morales-Rodriguez F, Cruys B, Treps L, Ramer L, Vinckier S, Brepoels K, Wyns S, Souffreau J, Schoonjans L, Lamers WH, Wu Y, Haustraete J, Hofkens J, Liekens S, Cubbon R, Ghesquière B, Dewerchin M, Gervasio FL, Li X, van Buul JD, Wu X, Carmeliet P
Nature561(7721)63-69(2018 09)

Dysregulation of endothelial colony-forming cell function by a negative feedback loop of circulating miR-146a and -146b in cardiovascular disease patients.
Chang TY, Tsai WC, Huang TS, Su SH, Chang CY, Ma HY, Wu CH, Yang CY, Lin CH, Huang PH, Cheng CC, Cheng SM, Wang HW
PloS one12(7)e0181562(2017)

TCL/RhoJ Plasma Membrane Localization and Nucleotide Exchange Is Coordinately Regulated by Amino Acids within the N Terminus and a Distal Loop Region.
Ackermann KL, Florke RR, Reyes SS, Tader BR, Hamann MJ
The Journal of biological chemistry291(45)23604-23617(2016 Nov)

Genome-wide identification of endothelial cell-enriched genes in the mouse embryo.
Takase H, Matsumoto K, Yamadera R, Kubota Y, Otsu A, Suzuki R, Ishitobi H, Mochizuki H, Kojima T, Takano S, Uchida K, Takahashi S, Ema M
Blood120(4)914-23(2012 Jul)

RhoJ is an endothelial cell-restricted Rho GTPase that mediates vascular morphogenesis and is regulated by the transcription factor ERG.
Yuan L, Sacharidou A, Stratman AN, Le Bras A, Zwiers PJ, Spokes K, Bhasin M, Shih SC, Nagy JA, Molema G, Aird WC, Davis GE, Oettgen P
Blood118(4)1145-53(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rhoj gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhoj cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11401	NM_001008320.1 Latest version!	Rattus norvegicus ras homolog family member J (Rhoj), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhoj ( NM_001008320.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008321.1 Rattus norvegicus ras homolog family member J (Rhoj), mRNA.

Price & Availability↑

CloneID	ORa11401
Clone ID Related Accession (Same CDS sequence)	NM_001008320.1
Accession Version	NM_001008320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	rho-related GTP-binding protein RhoJ
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086525.1. On Dec 16, 2004 this sequence version replaced XM_216737.2. ##Evidence-Data-START## Transcript exon combination :: BC086525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTGCA GAGAGGGGAC TGACAGCAGC TGCGGCTGCG GTGGGCGCGA TGAAAACAGG 
ATCCTGAAGT GCGTGGTGGT CGGGGACGGC GCGGTGGGGA AGACTTGCCT GCTGATGAGC 
TACGCCAACG ACGCCTTCCC GGAGGAATAC GTGCCCACTG TGTTTGACCA CTACGCAGTT 
ACCGTGACAG TGGGAGGCAA GCAGCACCTG CTTGGACTGT ACGACACCGC GGGACAGGAG 
GACTACAACC AGCTGCGGCC CCTCTCTTAC CCCAACACCG ACGTGTTTCT CATCTGCTTC 
TCTGTCGTGA ACCCTGCCTC TTACCACAAC GTTCAGGAGG AGTGGGTCCC GGAGCTGAAG 
GACTGCATGC CTCACGTGCC TTACGTGCTC ATTGGGACCC AGATTGATCT CCGAGATGAC 
CCCAAAACCT TGGCGCGTCT GCTGTACATG AAGGAGAAGC CCCTCACCTA TGAGCACGGC 
GTGAAACTGG CGAAAGCGAT AGGAGCTCAG TGCTACTTGG AATGCTCTGC CCTGACTCAG 
AAGGGTCTCA AAGCTGTTTT TGATGAAGCC ATCCTCACCA TTTTCCACCC CAAGAAAAAA 
AAGAAAGGCT GCCACGGCTG CTGTGTAATC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008321.1
CDS	265..900
Translation	MSCREGTDSSCGCGGRDENRILKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKGCHGCCVII

Target ORF information:

RefSeq Version	NM_001008320.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ras homolog family member J (Rhoj), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008320.1

ORF Insert Sequence:

ATGAGCTGCA GAGAGGGGAC TGACAGCAGC TGCGGCTGCG GTGGGCGCGA TGAAAACAGG 
ATCCTGAAGT GCGTGGTGGT CGGGGACGGC GCGGTGGGGA AGACTTGCCT GCTGATGAGC 
TACGCCAACG ACGCCTTCCC GGAGGAATAC GTGCCCACTG TGTTTGACCA CTACGCAGTT 
ACCGTGACAG TGGGAGGCAA GCAGCACCTG CTTGGACTGT ACGACACCGC GGGACAGGAG 
GACTACAACC AGCTGCGGCC CCTCTCTTAC CCCAACACCG ACGTGTTTCT CATCTGCTTC 
TCTGTCGTGA ACCCTGCCTC TTACCACAAC GTTCAGGAGG AGTGGGTCCC GGAGCTGAAG 
GACTGCATGC CTCACGTGCC TTACGTGCTC ATTGGGACCC AGATTGATCT CCGAGATGAC 
CCCAAAACCT TGGCGCGTCT GCTGTACATG AAGGAGAAGC CCCTCACCTA TGAGCACGGC 
GTGAAACTGG CGAAAGCGAT AGGAGCTCAG TGCTACTTGG AATGCTCTGC CCTGACTCAG 
AAGGGTCTCA AAGCTGTTTT TGATGAAGCC ATCCTCACCA TTTTCCACCC CAAGAAAAAA 
AAGAAAGGCT GCCACGGCTG CTGTGTAATC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of glutamine synthetase in angiogenesis beyond glutamine synthesis.
Nature561(7721)63-69(2018 09)
Eelen G,Dubois C,Cantelmo AR,Goveia J,Brüning U,DeRan M,Jarugumilli G,van Rijssel J,Saladino G,Comitani F,Zecchin A,Rocha S,Chen R,Huang H,Vandekeere S,Kalucka J,Lange C,Morales-Rodriguez F,Cruys B,Treps L,Ramer L,Vinckier S,Brepoels K,Wyns S,Souffreau J,Schoonjans L,Lamers WH,Wu Y,Haustraete J,Hofkens J,Liekens S,Cubbon R,Ghesquière B,Dewerchin M,Gervasio FL,Li X,van Buul JD,Wu X,Carmeliet P

Dysregulation of endothelial colony-forming cell function by a negative feedback loop of circulating miR-146a and -146b in cardiovascular disease patients.
PloS one12(7)e0181562(2017)
Chang TY,Tsai WC,Huang TS,Su SH,Chang CY,Ma HY,Wu CH,Yang CY,Lin CH,Huang PH,Cheng CC,Cheng SM,Wang HW

TCL/RhoJ Plasma Membrane Localization and Nucleotide Exchange Is Coordinately Regulated by Amino Acids within the N Terminus and a Distal Loop Region.
The Journal of biological chemistry291(45)23604-23617(2016 Nov)
Ackermann KL,Florke RR,Reyes SS,Tader BR,Hamann MJ

Genome-wide identification of endothelial cell-enriched genes in the mouse embryo.
Blood120(4)914-23(2012 Jul)
Takase H,Matsumoto K,Yamadera R,Kubota Y,Otsu A,Suzuki R,Ishitobi H,Mochizuki H,Kojima T,Takano S,Uchida K,Takahashi S,Ema M

RhoJ is an endothelial cell-restricted Rho GTPase that mediates vascular morphogenesis and is regulated by the transcription factor ERG.
Blood118(4)1145-53(2011 Jul)
Yuan L,Sacharidou A,Stratman AN,Le Bras A,Zwiers PJ,Spokes K,Bhasin M,Shih SC,Nagy JA,Molema G,Aird WC,Davis GE,Oettgen P

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Rhoj cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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