Hsd3b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd3b2
Entrez Gene ID	29632
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Synonyms	Hsd3b1,Hsd3b2l1,Hsd3b6
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 Names 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 4 3 beta-hydroxysteroid dehydrogenase 6 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type IV 3-beta-HSD IV Hydroxy-delta-5-steroid dehydrogenase 3 beta- and steroid delta-isomerase hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6 hydroxysteroid dehydrogenase-6 delta<5>-3-beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	enzyme responsible for the production of progesterone as well as the precursors of androgens, estrogens, glucocorticoids and mineralcorticoids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017265.4	NP_058961.4	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hsd3b	NP_001036084.1
Danio rerio (zebrafish)	si:rp71-68n21.10	XP_694204.3
Danio rerio (zebrafish)	hsd3b1	NP_997962.1
Xenopus tropicalis (tropical clawed frog)	LOC100490622	XP_004920800.1
Gallus gallus (chicken)	HSD3B2	NP_990449.1
Rattus norvegicus (Norway rat)	LOC100911154	XP_006233083.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006654	XP_316685.4
Mus musculus (house mouse)	Hsd3b1	NP_032319.1
Rattus norvegicus (Norway rat)	Hsd3b6	NP_058961.4
Macaca mulatta (Rhesus monkey)	LOC100424395	XP_002801771.1
Homo sapiens (human)	HSD3B2	NP_001159592.1
Pan troglodytes (chimpanzee)	HSD3B2	XP_001142555.1
Canis lupus familiaris (dog)	HSD3B2	NP_001010954.1
Bos taurus (cattle)	HSD3B1	NP_776768.1
Drosophila melanogaster (fruit fly)	CG7724	NP_648957.1

Gene Ontology
Bibliography

Related articles in PubMed

Semicomprehensive analysis of the postnatal age-related changes in the mRNA expression of sex steroidogenic enzymes and sex steroid receptors in the male rat hippocampus.
Kimoto T, Ishii H, Higo S, Hojo Y, Kawato S
Endocrinology151(12)5795-806(2010 Dec)

Studies on the protective role of lycopene against polychlorinated biphenyls (Aroclor 1254)-induced changes in StAR protein and cytochrome P450 scc enzyme expression on Leydig cells of adult rats.
Elumalai P, Krishnamoorthy G, Selvakumar K, Arunkumar R, Venkataraman P, Arunakaran J
Reproductive toxicology (Elmsford, N.Y.)27(1)41-5(2009 Jan)

Biochemical effects of gestational coexposure to lead and cadmium on reproductive performance, placenta, and ovary.
Nampoothiri LP, Gupta S
Journal of biochemical and molecular toxicology22(5)337-44(2008 Sep-Oct)

Leydig cell re-generation and expression of cell signaling molecules in the germ cell-free testis.
O'Shaughnessy PJ, Morris ID, Baker PJ
Reproduction (Cambridge, England)135(6)851-8(2008 Jun)

Expression of androgen receptor and 3beta-hydroxysteroid dehydrogenase in corpora lutea during pregnancy in the rat.
Szołtys M, Słomczyńska M, Galas J, Duda M, Sakiewicz A
Reproduction, fertility, and development19(2)356-65(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsd3b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd3b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03460	NM_017265.4 Latest version!	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (Hsd3b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd3b2 ( NM_017265.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058961.4 Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (Hsd3b2), mRNA.

Price & Availability↑

CloneID	ORa03460
Clone ID Related Accession (Same CDS sequence)	NM_017265.4
Accession Version	NM_017265.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089937.1. On Mar 27, 2005 this sequence version replaced NM_017265.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089937.1, FQ148380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGGGT GGAGCTGCCT GGTGACTGGA GCAGGAGGGT TTTTGGGCCA GAGGATCGTC 
CAGTTGTTGG TGCAGGAGAA AGATCTGAAG GAGGTCAGGG TCCTGGACAA GGTCTTCAGA 
CCAGAAACCA GAGAAGAATT CTTCAACCTA GGGACAAGCA TCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TTGACACCCA GTGCCTGAGG AGAGCGTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCCG CCCTCATTGA TGTCACAGGT GTCAATCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA TCTGTTGGAG GCCTGTGTCC AAGCTAGTGT GCCAGCCTTC 
ATCTACTGCA GCACAGTTGA CGTTGCAGGG CCCAACTCCT ACAAGAAGAT CATCCTGAAT 
GGCCATGAGG AAGAGCATCA TGAAAGCACA TGGTCAAATC CATACCCATA CAGCAAAAAG 
ATGGCCGAGA AGGCAGTGCT GGCAGCCAAT GGGAGCATCC TGAAAAATGG TGGCACATTG 
CATACTTGTG CCTTAAGGCC CATGTACATT TATGGGGAGA GAAGTCCATT CCTTTCTGTC 
ATGATACTTG CGGCCCTCAA AAGTAAGGGT ATTCTGAATG TTACTGGCAA ATTCTCCATA 
GCCAATCCAG TGTATGTAGG CAATGTGGCC TGGGCACATA TTCTGGCTGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCACA AAATGTCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAT ACCCTGAGCA AGGAATGGGG CCTCCACCTT 
GATTCCAGTT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAATT 
GTGAGCTTCT TTCTGCATCC AGTGTACAAC TATAGGCCAT CCTTTAACCG CCACTTGGTC 
ACACTGTCAA ATAGCAAGTT CACTTTCTCC TACAAGAAAG CTCAGAGAGA TCTGGGCTAT 
AAGCCACTTG TCAGCTGGGA GGAAGCCAAG CAGAAAACCT CGGAGTGGAT CGGGACACTA 
GTGGAGCAGC ACAGGGAGAC ATTGGACACA AAGTCTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058961.4
CDS	219..1340
Translation	MPGWSCLVTGAGGFLGQRIVQLLVQEKDLKEVRVLDKVFRPETREEFFNLGTSIKVTVLEGDILDTQCLRRACQGISVVIHTAALIDVTGVNPRQTILDVNLKGTQNLLEACVQASVPAFIYCSTVDVAGPNSYKKIILNGHEEEHHESTWSNPYPYSKKMAEKAVLAANGSILKNGGTLHTCALRPMYIYGERSPFLSVMILAALKSKGILNVTGKFSIANPVYVGNVAWAHILAARGLRDPKKSQNVQGQFYYISDDTPHQSYDDLNYTLSKEWGLHLDSSWSLPLPLLYWLAFLLEIVSFFLHPVYNYRPSFNRHLVTLSNSKFTFSYKKAQRDLGYKPLVSWEEAKQKTSEWIGTLVEQHRETLDTKSQ

Target ORF information:

RefSeq Version	NM_017265.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (Hsd3b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017265.4

ORF Insert Sequence:

ATGCCTGGGT GGAGCTGCCT GGTGACTGGA GCAGGAGGGT TTTTGGGCCA GAGGATCGTC 
CAGTTGTTGG TGCAGGAGAA AGATCTGAAG GAGGTCAGGG TCCTGGACAA GGTCTTCAGA 
CCAGAAACCA GAGAAGAATT CTTCAACCTA GGGACAAGCA TCAAGGTGAC AGTGTTGGAA 
GGAGACATTC TTGACACCCA GTGCCTGAGG AGAGCGTGCC AGGGCATCTC TGTTGTCATC 
CACACTGCCG CCCTCATTGA TGTCACAGGT GTCAATCCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA TCTGTTGGAG GCCTGTGTCC AAGCTAGTGT GCCAGCCTTC 
ATCTACTGCA GCACAGTTGA CGTTGCAGGG CCCAACTCCT ACAAGAAGAT CATCCTGAAT 
GGCCATGAGG AAGAGCATCA TGAAAGCACA TGGTCAAATC CATACCCATA CAGCAAAAAG 
ATGGCCGAGA AGGCAGTGCT GGCAGCCAAT GGGAGCATCC TGAAAAATGG TGGCACATTG 
CATACTTGTG CCTTAAGGCC CATGTACATT TATGGGGAGA GAAGTCCATT CCTTTCTGTC 
ATGATACTTG CGGCCCTCAA AAGTAAGGGT ATTCTGAATG TTACTGGCAA ATTCTCCATA 
GCCAATCCAG TGTATGTAGG CAATGTGGCC TGGGCACATA TTCTGGCTGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCACA AAATGTCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTAT ACCCTGAGCA AGGAATGGGG CCTCCACCTT 
GATTCCAGTT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAATT 
GTGAGCTTCT TTCTGCATCC AGTGTACAAC TATAGGCCAT CCTTTAACCG CCACTTGGTC 
ACACTGTCAA ATAGCAAGTT CACTTTCTCC TACAAGAAAG CTCAGAGAGA TCTGGGCTAT 
AAGCCACTTG TCAGCTGGGA GGAAGCCAAG CAGAAAACCT CGGAGTGGAT CGGGACACTA 
GTGGAGCAGC ACAGGGAGAC ATTGGACACA AAGTCTCAGT AA