March5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	March5
Entrez Gene ID	294079
Full Name	membrane associated ring-CH-type finger 5
Synonyms	Rnf153
General protein information	Preferred Names membrane associated ring-CH-type finger 5 Names E3 ubiquitin-protein ligase MARCH5 membrane-associated ring finger (C3HC4) 5 ring finger protein 153
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106372.2	NP_001099842.1	E3 ubiquitin-protein ligase MARCH5
XM_008760367.2	XP_008758589.1	E3 ubiquitin-protein ligase MARCH5 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MARCH5	NP_060294.1
Pan troglodytes (chimpanzee)	MARCH5	XP_001145688.1
Macaca mulatta (Rhesus monkey)	MARCH5	XP_001089783.1
Danio rerio (zebrafish)	march5	NP_001076296.1
Xenopus tropicalis (tropical clawed frog)	march5	NP_001116950.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001041	XP_309240.5
Canis lupus familiaris (dog)	MARCH5	XP_849261.1
Drosophila melanogaster (fruit fly)	CG9855	NP_649463.2
Bos taurus (cattle)	MARCH5	NP_001029887.1
Rattus norvegicus (Norway rat)	March5	NP_001099842.1
Gallus gallus (chicken)	MARCH5	NP_001012924.1
Mus musculus (house mouse)	March5	NP_081590.3

Gene Ontology
Bibliography

Related articles in PubMed

The mitochondrial ubiquitin ligase plays an anti-apoptotic role in cardiomyocytes by regulating mitochondrial fission.
Wang J, Aung LH, Prabhakar BS, Li P
Journal of cellular and molecular medicine20(12)2278-2288(2016 12)

Loss of MARCH5 mitochondrial E3 ubiquitin ligase induces cellular senescence through dynamin-related protein 1 and mitofusin 1.
Park YY, Lee S, Karbowski M, Neutzner A, Youle RJ, Cho H
Journal of cell science123(Pt 4)619-26(2010 Feb)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

The mitochondrial E3 ubiquitin ligase MARCH5 is required for Drp1 dependent mitochondrial division.
Karbowski M, Neutzner A, Youle RJ
The Journal of cell biology178(1)71-84(2007 Jul)

MARCH-V is a novel mitofusin 2- and Drp1-binding protein able to change mitochondrial morphology.
Nakamura N, Kimura Y, Tokuda M, Honda S, Hirose S
EMBO reports7(10)1019-22(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following March5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the March5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04660	NM_001106372.2 Latest version!	Rattus norvegicus membrane associated ring-CH-type finger 5 (Marchf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa24053	XM_008760367.2 Latest version!	Rattus norvegicus membrane associated ring-CH-type finger 5 (March5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

March5 ( NM_001106372.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099842.1 Rattus norvegicus membrane associated ring-CH-type finger 5 (Marchf5), mRNA.

Price & Availability↑

CloneID	ORa04660
Clone ID Related Accession (Same CDS sequence)	NM_001106372.2
Accession Version	NM_001106372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565539200000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase MARCH5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC158801.1. On Jan 27, 2008 this sequence version replaced NM_001106372.1. ##Evidence-Data-START## Transcript exon combination :: BC158801.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGACC AAGCCCTTCA ACAGATGCTG GACAGAAGTT GCTGGGTGTG TTTTGCCACC 
GATGAAGACG ATAGAACAGC TGAATGGGTG AGACCATGCA GGTGCAGAGG ATCTACTAAG 
TGGGTTCACC AGGCTTGTCT ACAACGCTGG GTAGATGAGA AGCAACGAGG AAACAGCACA 
GCCAGAGTGG CCTGCCCTCA GTGCAATGCC GAGTACTTAA TAGTGTTTCC AAAGCTGGGT 
CCAGTTGTTT ATGTCTTGGA TCTTGCAGAT AGACTGATCT CAAAAGCTTG CCCCTTTGCT 
GCAGCAGGAA TAATGGTTGG ATCTATCTAC TGGACAGCTG TGACTTATGG AGCAGTGACA 
GTGATGCAGG TTGTAGGCCA TAAAGAAGGG CTGGATGTTA TGGAGCGAGC TGACCCTTTA 
TTTCTTTTGA TTGGACTTCC TACTATTCCT GTCATGCTGA TACTAGGCAA AATGATTCGC 
TGGGAGGACT ATGTGCTTAG ACTATGGCGC AAATACTCAA ATAAACTACA AATTTTGAAC 
AGTATATTTC CAGGGATTGG TTGTCCTGTT CCTCGAATTC CAGCTGAAGC TAATCCTTTA 
GCAGATCATG TCTCTGCTAC TAGAATTTTG TGTGGAGCCC TTGTCTTTCC TACTATTGCG 
ACAATAGTTG GTAAACTGAT GTTCAGTAGC GTTAACTCAA ATTTACAGAG GACAATCTTG 
GGCGGAATTG CTTTTGTTGC CATAAAAGGA GCATTTAAGG TTTACTTCAA ACAGCAGCAA 
TATTTACGTC AGGCACACCG CAAAATCCTA AATTATCCAG AGCAAGAAGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099842.1
CDS	287..1123
Translation	MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEEA

Target ORF information:

RefSeq Version	NM_001106372.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated ring-CH-type finger 5 (Marchf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106372.2

ORF Insert Sequence:

ATGCCGGACC AAGCCCTTCA ACAGATGCTG GACAGAAGTT GCTGGGTGTG TTTTGCCACC 
GATGAAGACG ATAGAACAGC TGAATGGGTG AGACCATGCA GGTGCAGAGG ATCTACTAAG 
TGGGTTCACC AGGCTTGTCT ACAACGCTGG GTAGATGAGA AGCAACGAGG AAACAGCACA 
GCCAGAGTGG CCTGCCCTCA GTGCAATGCC GAGTACTTAA TAGTGTTTCC AAAGCTGGGT 
CCAGTTGTTT ATGTCTTGGA TCTTGCAGAT AGACTGATCT CAAAAGCTTG CCCCTTTGCT 
GCAGCAGGAA TAATGGTTGG ATCTATCTAC TGGACAGCTG TGACTTATGG AGCAGTGACA 
GTGATGCAGG TTGTAGGCCA TAAAGAAGGG CTGGATGTTA TGGAGCGAGC TGACCCTTTA 
TTTCTTTTGA TTGGACTTCC TACTATTCCT GTCATGCTGA TACTAGGCAA AATGATTCGC 
TGGGAGGACT ATGTGCTTAG ACTATGGCGC AAATACTCAA ATAAACTACA AATTTTGAAC 
AGTATATTTC CAGGGATTGG TTGTCCTGTT CCTCGAATTC CAGCTGAAGC TAATCCTTTA 
GCAGATCATG TCTCTGCTAC TAGAATTTTG TGTGGAGCCC TTGTCTTTCC TACTATTGCG 
ACAATAGTTG GTAAACTGAT GTTCAGTAGC GTTAACTCAA ATTTACAGAG GACAATCTTG 
GGCGGAATTG CTTTTGTTGC CATAAAAGGA GCATTTAAGG TTTACTTCAA ACAGCAGCAA 
TATTTACGTC AGGCACACCG CAAAATCCTA AATTATCCAG AGCAAGAAGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

March5 ( XM_008760367.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758589.1 Rattus norvegicus membrane associated ring-CH-type finger 5 (March5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24053
Clone ID Related Accession (Same CDS sequence)	XM_008760367.2
Accession Version	XM_008760367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase MARCH5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760367.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGACC AAGCCCTTCA ACAGATGCTG GACAGAAGTT GCTGGGTGTG TTTTGCCACC 
GATGAAGACG ATAGAACAGC TGAATGGGTG AGACCATGCA GGTGCAGAGG ATCTACTAAG 
TGGGTTCACC AGGCTTGTCT ACAACGCTGG GTAGATGAGA AGCAACGAGG AAACAGCACA 
GCCAGAGTGG CCTGCCCTCA GTGCAATGCC GAGTACTTAA TAGTGTTTCC AAAGCTGGGT 
CCAGTTGTTT ATGTCTTGGA TCTTGCAGAT AGACTGATCT CAAAAGCTTG CCCCTTTGCT 
GCAGCAGGAA TAATGGTTGG ATCTATCTAC TGGACAGCTG TGACTTATGG AGCAGTGACA 
GTGATGCAGG TTCACCATTC TACTCTATTC AGTGACACTA CTTTTGATGT GAAGGTTGTA 
GGCCATAAAG AAGGGCTGGA TGTTATGGAG CGAGCTGACC CTTTATTTCT TTTGATTGGA 
CTTCCTACTA TTCCTGTCAT GCTGATACTA GGCAAAATGA TTCGCTGGGA GGACTATGTG 
CTTAGACTAT GGCGCAAATA CTCAAATAAA CTACAAATTT TGAACAGTAT ATTTCCAGGG 
ATTGGTTGTC CTGTTCCTCG AATTCCAGCT GAAGCTAATC CTTTAGCAGA TCATGTCTCT 
GCTACTAGAA TTTTGTGTGG AGCCCTTGTC TTTCCTACTA TTGCGACAAT AGTTGGTAAA 
CTGATGTTCA GTAGCGTTAA CTCAAATTTA CAGAGGACAA TCTTGGGCGG AATTGCTTTT 
GTTGCCATAA AAGGAGCATT TAAGGTTTAC TTCAAACAGC AGCAATATTT ACGTCAGGCA 
CACCGCAAAA TCCTAAATTA TCCAGAGCAA GAAGAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758589.1
CDS	290..1171
Translation	MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVHHSTLFSDTTFDVKVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEEA

Target ORF information:

RefSeq Version	XM_008760367.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated ring-CH-type finger 5 (March5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760367.2

ORF Insert Sequence:

ATGCCGGACC AAGCCCTTCA ACAGATGCTG GACAGAAGTT GCTGGGTGTG TTTTGCCACC 
GATGAAGACG ATAGAACAGC TGAATGGGTG AGACCATGCA GGTGCAGAGG ATCTACTAAG 
TGGGTTCACC AGGCTTGTCT ACAACGCTGG GTAGATGAGA AGCAACGAGG AAACAGCACA 
GCCAGAGTGG CCTGCCCTCA GTGCAATGCC GAGTACTTAA TAGTGTTTCC AAAGCTGGGT 
CCAGTTGTTT ATGTCTTGGA TCTTGCAGAT AGACTGATCT CAAAAGCTTG CCCCTTTGCT 
GCAGCAGGAA TAATGGTTGG ATCTATCTAC TGGACAGCTG TGACTTATGG AGCAGTGACA 
GTGATGCAGG TTCACCATTC TACTCTATTC AGTGACACTA CTTTTGATGT GAAGGTTGTA 
GGCCATAAAG AAGGGCTGGA TGTTATGGAG CGAGCTGACC CTTTATTTCT TTTGATTGGA 
CTTCCTACTA TTCCTGTCAT GCTGATACTA GGCAAAATGA TTCGCTGGGA GGACTATGTG 
CTTAGACTAT GGCGCAAATA CTCAAATAAA CTACAAATTT TGAACAGTAT ATTTCCAGGG 
ATTGGTTGTC CTGTTCCTCG AATTCCAGCT GAAGCTAATC CTTTAGCAGA TCATGTCTCT 
GCTACTAGAA TTTTGTGTGG AGCCCTTGTC TTTCCTACTA TTGCGACAAT AGTTGGTAAA 
CTGATGTTCA GTAGCGTTAA CTCAAATTTA CAGAGGACAA TCTTGGGCGG AATTGCTTTT 
GTTGCCATAA AAGGAGCATT TAAGGTTTAC TTCAAACAGC AGCAATATTT ACGTCAGGCA 
CACCGCAAAA TCCTAAATTA TCCAGAGCAA GAAGAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mitochondrial ubiquitin ligase plays an anti-apoptotic role in cardiomyocytes by regulating mitochondrial fission.
Journal of cellular and molecular medicine20(12)2278-2288(2016 12)
Wang J,Aung LH,Prabhakar BS,Li P

Loss of MARCH5 mitochondrial E3 ubiquitin ligase induces cellular senescence through dynamin-related protein 1 and mitofusin 1.
Journal of cell science123(Pt 4)619-26(2010 Feb)
Park YY,Lee S,Karbowski M,Neutzner A,Youle RJ,Cho H

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

The mitochondrial E3 ubiquitin ligase MARCH5 is required for Drp1 dependent mitochondrial division.
The Journal of cell biology178(1)71-84(2007 Jul)
Karbowski M,Neutzner A,Youle RJ

MARCH-V is a novel mitofusin 2- and Drp1-binding protein able to change mitochondrial morphology.
EMBO reports7(10)1019-22(2006 Oct)
Nakamura N,Kimura Y,Tokuda M,Honda S,Hirose S