Rnmt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnmt
Entrez Gene ID	291534
Full Name	RNA (guanine-7-) methyltransferase
General protein information	Preferred Names RNA (guanine-7-) methyltransferase Names mRNA cap guanine-N7 methyltransferase RG7MT1 mRNA (guanine-N(7)-)-methyltransferase mRNA cap methyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006254894.1	XP_006254956.1	mRNA cap guanine-N7 methyltransferase isoform X1
XM_006254895.3	XP_006254957.1	mRNA cap guanine-N7 methyltransferase isoform X1
NM_001008299.2	NP_001008300.1	mRNA cap guanine-N7 methyltransferase
XM_006254896.3	XP_006254958.1	mRNA cap guanine-N7 methyltransferase isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rnmt	NP_001008300.1
Xenopus tropicalis (tropical clawed frog)	rnmt	NP_001017053.1
Bos taurus (cattle)	RNMT	NP_001092412.1
Mus musculus (house mouse)	Rnmt	NP_080716.1
Gallus gallus (chicken)	RNMT	XP_419132.1
Drosophila melanogaster (fruit fly)	l(2)35Bd	NP_523568.2
Caenorhabditis elegans (roundworm)	tag-72	NP_492674.2
Homo sapiens (human)	RNMT	NP_003790.1
Pan troglodytes (chimpanzee)	RNMT	XP_001171239.1
Canis lupus familiaris (dog)	RNMT	XP_005615461.1
Danio rerio (zebrafish)	rnmt	NP_001038465.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003460	XP_311746.4
Macaca mulatta (Rhesus monkey)	LOC100430132	XP_002800903.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular basis of RNA guanine-7 methyltransferase (RNMT) activation by RAM.
Varshney D, Petit AP, Bueren-Calabuig JA, Jansen C, Fletcher DA, Peggie M, Weidlich S, Scullion P, Pisliakov AV, Cowling VH
Nucleic acids research44(21)10423-10436(2016 Dec)

Cap-binding complex (CBC).
Gonatopoulos-Pournatzis T, Cowling VH
The Biochemical journal457(2)231-42(2014 Jan)

Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.
Ghai R, Bugarcic A, Liu H, Norwood SJ, Skeldal S, Coulson EJ, Li SS, Teasdale RD, Collins BM
Proceedings of the National Academy of Sciences of the United States of America110(8)E643-52(2013 Feb)

RAM/Fam103a1 is required for mRNA cap methylation.
Gonatopoulos-Pournatzis T, Dunn S, Bounds R, Cowling VH
Molecular cell44(4)585-96(2011 Nov)

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
Tarantino C, Paolella G, Cozzuto L, Minopoli G, Pastore L, Parisi S, Russo T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rnmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11222	NM_001008299.2 Latest version!	Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa11222	XM_006254894.1 Latest version!	Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa11222	XM_006254895.3 Latest version!	Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa38478	XM_006254896.3 Latest version!	Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnmt ( NM_001008299.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001008300.1 Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), mRNA.

Price & Availability↑

CloneID	ORa11222
Clone ID Related Accession (Same CDS sequence)	XM_006254894.1 , XM_006254895.3 , NM_001008299.2
Accession Version	NM_001008299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	mRNA cap guanine-N7 methyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085858.1 and CN543861.1. On Oct 12, 2007 this sequence version replaced NM_001008299.1. ##Evidence-Data-START## CDS exon combination :: BC085858.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAGTT CTGTGAAAGC ATCTGTAGAT TCTGAGACAG AATCTTCTCC AGGTGTTAAT 
GAAACCGCTG CTGCGTCTGG GCAGAGGCTT TCTGAAAAGA CACGCCAGCA AGCAGATCAG 
CCGAAAACAC AAGATGATCT TGTAGAGCAA AATTCAAGTT ATGTGCAAGA TTCTCCATCC 
AAGAAAAGAA AACTAGATGT CGAAATCATC CTAGATGAAA AACACTCTGA GGATGATGGA 
GGTGCTTCAA AGAGAAGCAA ACTGGAGAGG GGTGGTGGGT CAGAAGATGA GCCTTCCCCT 
GGAGGGCTTA CCGAGAGAAA GCGAAAACTG CAGCCTCAGG ATGCCTTGGA AACTCAGACT 
CGGAAATTTC AGAAGCTGGA GGAAGGACAC AGCTCAGCCG TGGCCGCCCA CTATAACGAG 
CTTCAGGAAG TCGGCTTGGT GAAGCGGAGT CAAAGTCGCA TTTTCTACCT AAGAAACTTT 
AATAATTGGA TTAAAAGTAT TCTTATTGGG GAAATTTTAG AAAAGGTACG ACAAAGAAAA 
AACCGTGACA TCACTGTTTT GGACCTGGGA TGTGGAAAAG GTGGAGATTT GCTCAAGTGG 
AGGAAGGGGA GAATTAGCAG GCTAGTGTGC GCTGATATTG CCGATATTTC TATGAAGCAA 
TGTCAACAAC GCTACGAAGA CATGAAATGT CGTCGTGATA ATGAGTATAT TTTTAGTGCA 
GAATTTATAA CTGCTGACTG TTCAAAGGAA CTTTTGGTTG AAAAATTCCA TGATCCAGAA 
ATGTACTTTG ACATCTGCAG CTGTCAGTTT GCTTGCCATT ACTCCTTCGA GTCCTTGGAG 
CAGGCCGACA TGATGCTTAG AAACGCCTGT GGAAGGCTTA ACCCTGGCGG CTATTTTATT 
GGTACCACTC CCAATAGCTT CGAACTGATA AGACGCCTTG AAGCTTCAGA AACAGAATCA 
TTTGGAAATG AAATATACAC TGTGAAATTC CAGAAGAAAG GAAATTATCC TTTATTTGGC 
TGCAAATATG ACTTTAACTT GGAAGGTGTT GTGGATGTCC CTGAATTCTT GGTCTATTTT 
CCATTGCTAA CTGAGATGGC AAAGAAGTAT AATATGAAGC TAATCTATAA AAAAACATTT 
CTGGAGTTCT ATGAAGAAAA GATTAAGAAC AATGAAAACA AAATGCTCTT AAAACGGATG 
CAGGCCCTGG AGTCATACCC TGCGAACGAG AACTCTAAAC TGGCCTCTGA AAAGGCGGGC 
GACTACGCAC ATGCAGCCGA GTACATGAAG AACAGTCAAG TAAGGTTACC TCTGGGAACG 
TTAAGTAAGT CAGAATGGGA AGCTACAAGC ATTTACTTGG TTTTTGCCTT TGAGAAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008300.1
CDS	95..1480
Translation	MESSVKASVDSETESSPGVNETAAASGQRLSEKTRQQADQPKTQDDLVEQNSSYVQDSPSKKRKLDVEIILDEKHSEDDGGASKRSKLERGGGSEDEPSPGGLTERKRKLQPQDALETQTRKFQKLEEGHSSAVAAHYNELQEVGLVKRSQSRIFYLRNFNNWIKSILIGEILEKVRQRKNRDITVLDLGCGKGGDLLKWRKGRISRLVCADIADISMKQCQQRYEDMKCRRDNEYIFSAEFITADCSKELLVEKFHDPEMYFDICSCQFACHYSFESLEQADMMLRNACGRLNPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGNYPLFGCKYDFNLEGVVDVPEFLVYFPLLTEMAKKYNMKLIYKKTFLEFYEEKIKNNENKMLLKRMQALESYPANENSKLASEKAGDYAHAAEYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ

Target ORF information:

RefSeq Version	NM_001008299.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008299.2

ORF Insert Sequence:

ATGGAAAGTT CTGTGAAAGC ATCTGTAGAT TCTGAGACAG AATCTTCTCC AGGTGTTAAT 
GAAACCGCTG CTGCGTCTGG GCAGAGGCTT TCTGAAAAGA CACGCCAGCA AGCAGATCAG 
CCGAAAACAC AAGATGATCT TGTAGAGCAA AATTCAAGTT ATGTGCAAGA TTCTCCATCC 
AAGAAAAGAA AACTAGATGT CGAAATCATC CTAGATGAAA AACACTCTGA GGATGATGGA 
GGTGCTTCAA AGAGAAGCAA ACTGGAGAGG GGTGGTGGGT CAGAAGATGA GCCTTCCCCT 
GGAGGGCTTA CCGAGAGAAA GCGAAAACTG CAGCCTCAGG ATGCCTTGGA AACTCAGACT 
CGGAAATTTC AGAAGCTGGA GGAAGGACAC AGCTCAGCCG TGGCCGCCCA CTATAACGAG 
CTTCAGGAAG TCGGCTTGGT GAAGCGGAGT CAAAGTCGCA TTTTCTACCT AAGAAACTTT 
AATAATTGGA TTAAAAGTAT TCTTATTGGG GAAATTTTAG AAAAGGTACG ACAAAGAAAA 
AACCGTGACA TCACTGTTTT GGACCTGGGA TGTGGAAAAG GTGGAGATTT GCTCAAGTGG 
AGGAAGGGGA GAATTAGCAG GCTAGTGTGC GCTGATATTG CCGATATTTC TATGAAGCAA 
TGTCAACAAC GCTACGAAGA CATGAAATGT CGTCGTGATA ATGAGTATAT TTTTAGTGCA 
GAATTTATAA CTGCTGACTG TTCAAAGGAA CTTTTGGTTG AAAAATTCCA TGATCCAGAA 
ATGTACTTTG ACATCTGCAG CTGTCAGTTT GCTTGCCATT ACTCCTTCGA GTCCTTGGAG 
CAGGCCGACA TGATGCTTAG AAACGCCTGT GGAAGGCTTA ACCCTGGCGG CTATTTTATT 
GGTACCACTC CCAATAGCTT CGAACTGATA AGACGCCTTG AAGCTTCAGA AACAGAATCA 
TTTGGAAATG AAATATACAC TGTGAAATTC CAGAAGAAAG GAAATTATCC TTTATTTGGC 
TGCAAATATG ACTTTAACTT GGAAGGTGTT GTGGATGTCC CTGAATTCTT GGTCTATTTT 
CCATTGCTAA CTGAGATGGC AAAGAAGTAT AATATGAAGC TAATCTATAA AAAAACATTT 
CTGGAGTTCT ATGAAGAAAA GATTAAGAAC AATGAAAACA AAATGCTCTT AAAACGGATG 
CAGGCCCTGG AGTCATACCC TGCGAACGAG AACTCTAAAC TGGCCTCTGA AAAGGCGGGC 
GACTACGCAC ATGCAGCCGA GTACATGAAG AACAGTCAAG TAAGGTTACC TCTGGGAACG 
TTAAGTAAGT CAGAATGGGA AGCTACAAGC ATTTACTTGG TTTTTGCCTT TGAGAAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnmt ( XM_006254894.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254956.1 Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11222
Clone ID Related Accession (Same CDS sequence)	XM_006254894.1 , XM_006254895.3 , NM_001008299.2
Accession Version	XM_006254894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	mRNA cap guanine-N7 methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTT CTGTGAAAGC ATCTGTAGAT TCTGAGACAG AATCTTCTCC AGGTGTTAAT 
GAAACCGCTG CTGCGTCTGG GCAGAGGCTT TCTGAAAAGA CACGCCAGCA AGCAGATCAG 
CCGAAAACAC AAGATGATCT TGTAGAGCAA AATTCAAGTT ATGTGCAAGA TTCTCCATCC 
AAGAAAAGAA AACTAGATGT CGAAATCATC CTAGATGAAA AACACTCTGA GGATGATGGA 
GGTGCTTCAA AGAGAAGCAA ACTGGAGAGG GGTGGTGGGT CAGAAGATGA GCCTTCCCCT 
GGAGGGCTTA CCGAGAGAAA GCGAAAACTG CAGCCTCAGG ATGCCTTGGA AACTCAGACT 
CGGAAATTTC AGAAGCTGGA GGAAGGACAC AGCTCAGCCG TGGCCGCCCA CTATAACGAG 
CTTCAGGAAG TCGGCTTGGT GAAGCGGAGT CAAAGTCGCA TTTTCTACCT AAGAAACTTT 
AATAATTGGA TTAAAAGTAT TCTTATTGGG GAAATTTTAG AAAAGGTACG ACAAAGAAAA 
AACCGTGACA TCACTGTTTT GGACCTGGGA TGTGGAAAAG GTGGAGATTT GCTCAAGTGG 
AGGAAGGGGA GAATTAGCAG GCTAGTGTGC GCTGATATTG CCGATATTTC TATGAAGCAA 
TGTCAACAAC GCTACGAAGA CATGAAATGT CGTCGTGATA ATGAGTATAT TTTTAGTGCA 
GAATTTATAA CTGCTGACTG TTCAAAGGAA CTTTTGGTTG AAAAATTCCA TGATCCAGAA 
ATGTACTTTG ACATCTGCAG CTGTCAGTTT GCTTGCCATT ACTCCTTCGA GTCCTTGGAG 
CAGGCCGACA TGATGCTTAG AAACGCCTGT GGAAGGCTTA ACCCTGGCGG CTATTTTATT 
GGTACCACTC CCAATAGCTT CGAACTGATA AGACGCCTTG AAGCTTCAGA AACAGAATCA 
TTTGGAAATG AAATATACAC TGTGAAATTC CAGAAGAAAG GAAATTATCC TTTATTTGGC 
TGCAAATATG ACTTTAACTT GGAAGGTGTT GTGGATGTCC CTGAATTCTT GGTCTATTTT 
CCATTGCTAA CTGAGATGGC AAAGAAGTAT AATATGAAGC TAATCTATAA AAAAACATTT 
CTGGAGTTCT ATGAAGAAAA GATTAAGAAC AATGAAAACA AAATGCTCTT AAAACGGATG 
CAGGCCCTGG AGTCATACCC TGCGAACGAG AACTCTAAAC TGGCCTCTGA AAAGGCGGGC 
GACTACGCAC ATGCAGCCGA GTACATGAAG AACAGTCAAG TAAGGTTACC TCTGGGAACG 
TTAAGTAAGT CAGAATGGGA AGCTACAAGC ATTTACTTGG TTTTTGCCTT TGAGAAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254956.1
CDS	256..1641
Translation	MESSVKASVDSETESSPGVNETAAASGQRLSEKTRQQADQPKTQDDLVEQNSSYVQDSPSKKRKLDVEIILDEKHSEDDGGASKRSKLERGGGSEDEPSPGGLTERKRKLQPQDALETQTRKFQKLEEGHSSAVAAHYNELQEVGLVKRSQSRIFYLRNFNNWIKSILIGEILEKVRQRKNRDITVLDLGCGKGGDLLKWRKGRISRLVCADIADISMKQCQQRYEDMKCRRDNEYIFSAEFITADCSKELLVEKFHDPEMYFDICSCQFACHYSFESLEQADMMLRNACGRLNPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGNYPLFGCKYDFNLEGVVDVPEFLVYFPLLTEMAKKYNMKLIYKKTFLEFYEEKIKNNENKMLLKRMQALESYPANENSKLASEKAGDYAHAAEYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ

Target ORF information:

RefSeq Version	XM_006254894.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254894.1

ORF Insert Sequence:

ATGGAAAGTT CTGTGAAAGC ATCTGTAGAT TCTGAGACAG AATCTTCTCC AGGTGTTAAT 
GAAACCGCTG CTGCGTCTGG GCAGAGGCTT TCTGAAAAGA CACGCCAGCA AGCAGATCAG 
CCGAAAACAC AAGATGATCT TGTAGAGCAA AATTCAAGTT ATGTGCAAGA TTCTCCATCC 
AAGAAAAGAA AACTAGATGT CGAAATCATC CTAGATGAAA AACACTCTGA GGATGATGGA 
GGTGCTTCAA AGAGAAGCAA ACTGGAGAGG GGTGGTGGGT CAGAAGATGA GCCTTCCCCT 
GGAGGGCTTA CCGAGAGAAA GCGAAAACTG CAGCCTCAGG ATGCCTTGGA AACTCAGACT 
CGGAAATTTC AGAAGCTGGA GGAAGGACAC AGCTCAGCCG TGGCCGCCCA CTATAACGAG 
CTTCAGGAAG TCGGCTTGGT GAAGCGGAGT CAAAGTCGCA TTTTCTACCT AAGAAACTTT 
AATAATTGGA TTAAAAGTAT TCTTATTGGG GAAATTTTAG AAAAGGTACG ACAAAGAAAA 
AACCGTGACA TCACTGTTTT GGACCTGGGA TGTGGAAAAG GTGGAGATTT GCTCAAGTGG 
AGGAAGGGGA GAATTAGCAG GCTAGTGTGC GCTGATATTG CCGATATTTC TATGAAGCAA 
TGTCAACAAC GCTACGAAGA CATGAAATGT CGTCGTGATA ATGAGTATAT TTTTAGTGCA 
GAATTTATAA CTGCTGACTG TTCAAAGGAA CTTTTGGTTG AAAAATTCCA TGATCCAGAA 
ATGTACTTTG ACATCTGCAG CTGTCAGTTT GCTTGCCATT ACTCCTTCGA GTCCTTGGAG 
CAGGCCGACA TGATGCTTAG AAACGCCTGT GGAAGGCTTA ACCCTGGCGG CTATTTTATT 
GGTACCACTC CCAATAGCTT CGAACTGATA AGACGCCTTG AAGCTTCAGA AACAGAATCA 
TTTGGAAATG AAATATACAC TGTGAAATTC CAGAAGAAAG GAAATTATCC TTTATTTGGC 
TGCAAATATG ACTTTAACTT GGAAGGTGTT GTGGATGTCC CTGAATTCTT GGTCTATTTT 
CCATTGCTAA CTGAGATGGC AAAGAAGTAT AATATGAAGC TAATCTATAA AAAAACATTT 
CTGGAGTTCT ATGAAGAAAA GATTAAGAAC AATGAAAACA AAATGCTCTT AAAACGGATG 
CAGGCCCTGG AGTCATACCC TGCGAACGAG AACTCTAAAC TGGCCTCTGA AAAGGCGGGC 
GACTACGCAC ATGCAGCCGA GTACATGAAG AACAGTCAAG TAAGGTTACC TCTGGGAACG 
TTAAGTAAGT CAGAATGGGA AGCTACAAGC ATTTACTTGG TTTTTGCCTT TGAGAAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnmt ( XM_006254895.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254957.1 Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa11222
Clone ID Related Accession (Same CDS sequence)	XM_006254894.1 , XM_006254895.3 , NM_001008299.2
Accession Version	XM_006254895.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	mRNA cap guanine-N7 methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254895.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTT CTGTGAAAGC ATCTGTAGAT TCTGAGACAG AATCTTCTCC AGGTGTTAAT 
GAAACCGCTG CTGCGTCTGG GCAGAGGCTT TCTGAAAAGA CACGCCAGCA AGCAGATCAG 
CCGAAAACAC AAGATGATCT TGTAGAGCAA AATTCAAGTT ATGTGCAAGA TTCTCCATCC 
AAGAAAAGAA AACTAGATGT CGAAATCATC CTAGATGAAA AACACTCTGA GGATGATGGA 
GGTGCTTCAA AGAGAAGCAA ACTGGAGAGG GGTGGTGGGT CAGAAGATGA GCCTTCCCCT 
GGAGGGCTTA CCGAGAGAAA GCGAAAACTG CAGCCTCAGG ATGCCTTGGA AACTCAGACT 
CGGAAATTTC AGAAGCTGGA GGAAGGACAC AGCTCAGCCG TGGCCGCCCA CTATAACGAG 
CTTCAGGAAG TCGGCTTGGT GAAGCGGAGT CAAAGTCGCA TTTTCTACCT AAGAAACTTT 
AATAATTGGA TTAAAAGTAT TCTTATTGGG GAAATTTTAG AAAAGGTACG ACAAAGAAAA 
AACCGTGACA TCACTGTTTT GGACCTGGGA TGTGGAAAAG GTGGAGATTT GCTCAAGTGG 
AGGAAGGGGA GAATTAGCAG GCTAGTGTGC GCTGATATTG CCGATATTTC TATGAAGCAA 
TGTCAACAAC GCTACGAAGA CATGAAATGT CGTCGTGATA ATGAGTATAT TTTTAGTGCA 
GAATTTATAA CTGCTGACTG TTCAAAGGAA CTTTTGGTTG AAAAATTCCA TGATCCAGAA 
ATGTACTTTG ACATCTGCAG CTGTCAGTTT GCTTGCCATT ACTCCTTCGA GTCCTTGGAG 
CAGGCCGACA TGATGCTTAG AAACGCCTGT GGAAGGCTTA ACCCTGGCGG CTATTTTATT 
GGTACCACTC CCAATAGCTT CGAACTGATA AGACGCCTTG AAGCTTCAGA AACAGAATCA 
TTTGGAAATG AAATATACAC TGTGAAATTC CAGAAGAAAG GAAATTATCC TTTATTTGGC 
TGCAAATATG ACTTTAACTT GGAAGGTGTT GTGGATGTCC CTGAATTCTT GGTCTATTTT 
CCATTGCTAA CTGAGATGGC AAAGAAGTAT AATATGAAGC TAATCTATAA AAAAACATTT 
CTGGAGTTCT ATGAAGAAAA GATTAAGAAC AATGAAAACA AAATGCTCTT AAAACGGATG 
CAGGCCCTGG AGTCATACCC TGCGAACGAG AACTCTAAAC TGGCCTCTGA AAAGGCGGGC 
GACTACGCAC ATGCAGCCGA GTACATGAAG AACAGTCAAG TAAGGTTACC TCTGGGAACG 
TTAAGTAAGT CAGAATGGGA AGCTACAAGC ATTTACTTGG TTTTTGCCTT TGAGAAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254957.1
CDS	1529..2914
Translation	MESSVKASVDSETESSPGVNETAAASGQRLSEKTRQQADQPKTQDDLVEQNSSYVQDSPSKKRKLDVEIILDEKHSEDDGGASKRSKLERGGGSEDEPSPGGLTERKRKLQPQDALETQTRKFQKLEEGHSSAVAAHYNELQEVGLVKRSQSRIFYLRNFNNWIKSILIGEILEKVRQRKNRDITVLDLGCGKGGDLLKWRKGRISRLVCADIADISMKQCQQRYEDMKCRRDNEYIFSAEFITADCSKELLVEKFHDPEMYFDICSCQFACHYSFESLEQADMMLRNACGRLNPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGNYPLFGCKYDFNLEGVVDVPEFLVYFPLLTEMAKKYNMKLIYKKTFLEFYEEKIKNNENKMLLKRMQALESYPANENSKLASEKAGDYAHAAEYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ

Target ORF information:

RefSeq Version	XM_006254895.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254895.3

ORF Insert Sequence:

ATGGAAAGTT CTGTGAAAGC ATCTGTAGAT TCTGAGACAG AATCTTCTCC AGGTGTTAAT 
GAAACCGCTG CTGCGTCTGG GCAGAGGCTT TCTGAAAAGA CACGCCAGCA AGCAGATCAG 
CCGAAAACAC AAGATGATCT TGTAGAGCAA AATTCAAGTT ATGTGCAAGA TTCTCCATCC 
AAGAAAAGAA AACTAGATGT CGAAATCATC CTAGATGAAA AACACTCTGA GGATGATGGA 
GGTGCTTCAA AGAGAAGCAA ACTGGAGAGG GGTGGTGGGT CAGAAGATGA GCCTTCCCCT 
GGAGGGCTTA CCGAGAGAAA GCGAAAACTG CAGCCTCAGG ATGCCTTGGA AACTCAGACT 
CGGAAATTTC AGAAGCTGGA GGAAGGACAC AGCTCAGCCG TGGCCGCCCA CTATAACGAG 
CTTCAGGAAG TCGGCTTGGT GAAGCGGAGT CAAAGTCGCA TTTTCTACCT AAGAAACTTT 
AATAATTGGA TTAAAAGTAT TCTTATTGGG GAAATTTTAG AAAAGGTACG ACAAAGAAAA 
AACCGTGACA TCACTGTTTT GGACCTGGGA TGTGGAAAAG GTGGAGATTT GCTCAAGTGG 
AGGAAGGGGA GAATTAGCAG GCTAGTGTGC GCTGATATTG CCGATATTTC TATGAAGCAA 
TGTCAACAAC GCTACGAAGA CATGAAATGT CGTCGTGATA ATGAGTATAT TTTTAGTGCA 
GAATTTATAA CTGCTGACTG TTCAAAGGAA CTTTTGGTTG AAAAATTCCA TGATCCAGAA 
ATGTACTTTG ACATCTGCAG CTGTCAGTTT GCTTGCCATT ACTCCTTCGA GTCCTTGGAG 
CAGGCCGACA TGATGCTTAG AAACGCCTGT GGAAGGCTTA ACCCTGGCGG CTATTTTATT 
GGTACCACTC CCAATAGCTT CGAACTGATA AGACGCCTTG AAGCTTCAGA AACAGAATCA 
TTTGGAAATG AAATATACAC TGTGAAATTC CAGAAGAAAG GAAATTATCC TTTATTTGGC 
TGCAAATATG ACTTTAACTT GGAAGGTGTT GTGGATGTCC CTGAATTCTT GGTCTATTTT 
CCATTGCTAA CTGAGATGGC AAAGAAGTAT AATATGAAGC TAATCTATAA AAAAACATTT 
CTGGAGTTCT ATGAAGAAAA GATTAAGAAC AATGAAAACA AAATGCTCTT AAAACGGATG 
CAGGCCCTGG AGTCATACCC TGCGAACGAG AACTCTAAAC TGGCCTCTGA AAAGGCGGGC 
GACTACGCAC ATGCAGCCGA GTACATGAAG AACAGTCAAG TAAGGTTACC TCTGGGAACG 
TTAAGTAAGT CAGAATGGGA AGCTACAAGC ATTTACTTGG TTTTTGCCTT TGAGAAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnmt ( XM_006254896.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254958.1 Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X3, mRNA.

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CloneID	ORa38478
Clone ID Related Accession (Same CDS sequence)	XM_006254896.3
Accession Version	XM_006254896.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	mRNA cap guanine-N7 methyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254896.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTT CTGTGAAAGC ATCTGTAGAT TCTGAGACAG AATCTTCTCC AGGTGTTAAT 
GAAACCGCTG CTGCGTCTGG GCAGAGGCTT TCTGAAAAGA CACGCCAGCA AGCAGATCAG 
CCGAAAACAC AAGATGATCT TGTAGAGCAA AATTCAAGTT ATGTGCAAGA TTCTCCATCC 
AAGAAAAGAA AACTAGATGT CGAAATCATC CTAGATGAAA AACACTCTGA GGATGATGGA 
GGTGCTTCAA AGAGAAGCAA ACTGGAGAGG GGTGGTGGGT CAGAAGATGA GCCTTCCCCT 
GGAGGGCTTA CCGAGAGAAA GCGAAAACTG CAGCCTCAGG ATGCCTTGGA AACTCAGACT 
CGGAAATTTC AGAAGCTGGA GGAAGGACAC AGCTCAGCCG TGGCCGCCCA CTATAACGAG 
CTTCAGGAAG TCGGCTTGGT GAAGCGGAGT CAAAGTCGCA TTTTCTACCT AAGAAACTTT 
AATAATTGGA TTAAAAGTAT TCTTATTGGG GAAATTTTAG AAAAGGTACG ACAAAGAAAA 
AACCGTGACA TCACTGTTTT GGACCTGGGA TGTGGAAAAG GTGGAGATTT GCTCAAGTGG 
AGGAAGGGGA GAATTAGCAG GCTAGTGTGC GCTGATATTG CCGATATTTC TATGAAGCAA 
TGTCAACAAC GCTACGAAGA CATGAAATGT CGTCGTGATA ATGAGTATAT TTTTAGTGCA 
GAATTTATAA CTGCTGACTG TTCAAAGGAA CTTTTGGTTG AAAAATTCCA TGATCCAGAA 
ATGTACTTTG ACATCTGCAG CTGTCAGTTT GCTTGCCATT ACTCCTTCGA GTCCTTGGAG 
CAGGCCGACA TGATGCTTAG AAACGCCTGT GGAAGGCTTA ACCCTGGCGG CTATTTTATT 
GGTACCACTC CCAATAGCTT CGAACTGATG ATGGCAAAGA AGTATAATAT GAAGCTAATC 
TATAAAAAAA CATTTCTGGA GTTCTATGAA GAAAAGATTA AGAACAATGA AAACAAAATG 
CTCTTAAAAC GGATGCAGGC CCTGGAGTCA TACCCTGCGA ACGAGAACTC TAAACTGGCC 
TCTGAAAAGG CGGGCGACTA CGCACATGCA GCCGAGTACA TGAAGAACAG TCAAGTAAGG 
TTACCTCTGG GAACGTTAAG TAAGTCAGAA TGGGAAGCTA CAAGCATTTA CTTGGTTTTT 
GCCTTTGAGA AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254958.1
CDS	144..1364
Translation	MESSVKASVDSETESSPGVNETAAASGQRLSEKTRQQADQPKTQDDLVEQNSSYVQDSPSKKRKLDVEIILDEKHSEDDGGASKRSKLERGGGSEDEPSPGGLTERKRKLQPQDALETQTRKFQKLEEGHSSAVAAHYNELQEVGLVKRSQSRIFYLRNFNNWIKSILIGEILEKVRQRKNRDITVLDLGCGKGGDLLKWRKGRISRLVCADIADISMKQCQQRYEDMKCRRDNEYIFSAEFITADCSKELLVEKFHDPEMYFDICSCQFACHYSFESLEQADMMLRNACGRLNPGGYFIGTTPNSFELMMAKKYNMKLIYKKTFLEFYEEKIKNNENKMLLKRMQALESYPANENSKLASEKAGDYAHAAEYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ

Target ORF information:

RefSeq Version	XM_006254896.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA (guanine-7-) methyltransferase (Rnmt), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254896.3

ORF Insert Sequence:

ATGGAAAGTT CTGTGAAAGC ATCTGTAGAT TCTGAGACAG AATCTTCTCC AGGTGTTAAT 
GAAACCGCTG CTGCGTCTGG GCAGAGGCTT TCTGAAAAGA CACGCCAGCA AGCAGATCAG 
CCGAAAACAC AAGATGATCT TGTAGAGCAA AATTCAAGTT ATGTGCAAGA TTCTCCATCC 
AAGAAAAGAA AACTAGATGT CGAAATCATC CTAGATGAAA AACACTCTGA GGATGATGGA 
GGTGCTTCAA AGAGAAGCAA ACTGGAGAGG GGTGGTGGGT CAGAAGATGA GCCTTCCCCT 
GGAGGGCTTA CCGAGAGAAA GCGAAAACTG CAGCCTCAGG ATGCCTTGGA AACTCAGACT 
CGGAAATTTC AGAAGCTGGA GGAAGGACAC AGCTCAGCCG TGGCCGCCCA CTATAACGAG 
CTTCAGGAAG TCGGCTTGGT GAAGCGGAGT CAAAGTCGCA TTTTCTACCT AAGAAACTTT 
AATAATTGGA TTAAAAGTAT TCTTATTGGG GAAATTTTAG AAAAGGTACG ACAAAGAAAA 
AACCGTGACA TCACTGTTTT GGACCTGGGA TGTGGAAAAG GTGGAGATTT GCTCAAGTGG 
AGGAAGGGGA GAATTAGCAG GCTAGTGTGC GCTGATATTG CCGATATTTC TATGAAGCAA 
TGTCAACAAC GCTACGAAGA CATGAAATGT CGTCGTGATA ATGAGTATAT TTTTAGTGCA 
GAATTTATAA CTGCTGACTG TTCAAAGGAA CTTTTGGTTG AAAAATTCCA TGATCCAGAA 
ATGTACTTTG ACATCTGCAG CTGTCAGTTT GCTTGCCATT ACTCCTTCGA GTCCTTGGAG 
CAGGCCGACA TGATGCTTAG AAACGCCTGT GGAAGGCTTA ACCCTGGCGG CTATTTTATT 
GGTACCACTC CCAATAGCTT CGAACTGATG ATGGCAAAGA AGTATAATAT GAAGCTAATC 
TATAAAAAAA CATTTCTGGA GTTCTATGAA GAAAAGATTA AGAACAATGA AAACAAAATG 
CTCTTAAAAC GGATGCAGGC CCTGGAGTCA TACCCTGCGA ACGAGAACTC TAAACTGGCC 
TCTGAAAAGG CGGGCGACTA CGCACATGCA GCCGAGTACA TGAAGAACAG TCAAGTAAGG 
TTACCTCTGG GAACGTTAAG TAAGTCAGAA TGGGAAGCTA CAAGCATTTA CTTGGTTTTT 
GCCTTTGAGA AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular basis of RNA guanine-7 methyltransferase (RNMT) activation by RAM.
Nucleic acids research44(21)10423-10436(2016 Dec)
Varshney D,Petit AP,Bueren-Calabuig JA,Jansen C,Fletcher DA,Peggie M,Weidlich S,Scullion P,Pisliakov AV,Cowling VH

Cap-binding complex (CBC).
The Biochemical journal457(2)231-42(2014 Jan)
Gonatopoulos-Pournatzis T,Cowling VH

Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.
Proceedings of the National Academy of Sciences of the United States of America110(8)E643-52(2013 Feb)
Ghai R,Bugarcic A,Liu H,Norwood SJ,Skeldal S,Coulson EJ,Li SS,Teasdale RD,Collins BM

RAM/Fam103a1 is required for mRNA cap methylation.
Molecular cell44(4)585-96(2011 Nov)
Gonatopoulos-Pournatzis T,Dunn S,Bounds R,Cowling VH

miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(9)3255-63(2010 Sep)
Tarantino C,Paolella G,Cozzuto L,Minopoli G,Pastore L,Parisi S,Russo T

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Rnmt cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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