Nanos3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nanos3
Entrez Gene ID	288909
Full Name	nanos C2HC-type zinc finger 3
General protein information	Preferred Names nanos C2HC-type zinc finger 3 Names nanos homolog 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105945.1	NP_001099415.1	nanos homolog 3
XM_006255235.3	XP_006255297.1	nanos homolog 3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NANOS3	NP_001092092.1
Pan troglodytes (chimpanzee)	NANOS3	XP_001171060.1
Mus musculus (house mouse)	Nanos3	NP_918948.1
Rattus norvegicus (Norway rat)	Nanos3	NP_001099415.1
Danio rerio (zebrafish)	nanos3	NP_571953.1
Macaca mulatta (Rhesus monkey)	NANOS3	XP_001111100.2
Canis lupus familiaris (dog)	NANOS3	XP_003432960.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression.
Bhandari D, Raisch T, Weichenrieder O, Jonas S, Izaurralde E
Genes & development28(8)888-901(2014 Apr)

Analysis of meiosis regulators in human gonads: a sexually dimorphic spatio-temporal expression pattern suggests involvement of DMRT1 in meiotic entry.
Jørgensen A, Nielsen JE, Blomberg Jensen M, Græm N, Rajpert-De Meyts E
Molecular human reproduction18(11)523-34(2012 Nov)

NANOS3 function in human germ cell development.
Julaton VT, Reijo Pera RA
Human molecular genetics20(11)2238-50(2011 Jun)

Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3.
Yamaji M, Tanaka T, Shigeta M, Chuma S, Saga Y, Saitou M
Reproduction (Cambridge, England)139(2)381-93(2010 Feb)

Nanos3 maintains the germ cell lineage in the mouse by suppressing both Bax-dependent and -independent apoptotic pathways.
Suzuki H, Tsuda M, Kiso M, Saga Y
Developmental biology318(1)133-42(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nanos3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nanos3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa41489	NM_001105945.1 Latest version!	Rattus norvegicus nanos C2HC-type zinc finger 3 (Nanos3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ORa38686	XM_006255235.3 Latest version!	Rattus norvegicus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nanos3 ( NM_001105945.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099415.1 Rattus norvegicus nanos C2HC-type zinc finger 3 (Nanos3), mRNA.

Price & Availability↑

CloneID	ORa41489
Clone ID Related Accession (Same CDS sequence)	NM_001105945.1
Accession Version	NM_001105945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	nanos homolog 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473972.1. On or before Oct 3, 2007 this sequence version replaced XM_001072052.1, XM_222459.3.

ATGGGAACTT TTAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATGAGGAAG AGGAGCCTGA TGTCAGGCTG GACCCCAAAC CAGAGCCCAA GCCAAGCTCA 
GCAAGCCAGC AAGCCAGCAA GGAATCCTCA GCAGCTCCAG AACGCCTATG TTCATTCTGC 
AAACACAATG GCGAGTCTCG TGCGATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATCTT GCGAGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT CTGCCCACTC ACCGGCCAGG GCTATACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
AATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTG TCTATGCGGG TTCCAAAAGT 
GGCAGGAAGC CCCCTGGACC TTCACCCTCG GCCTGCTGTC CCTCCACTAC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099415.1
CDS	25..561
Translation	MGTFNLWTDYLGLARLVGALHEEEEPDVRLDPKPEPKPSSASQQASKESSAAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATQEHAHTRRFCPLTGQGYTSVYCYTTRNSAGKKLTRPDKAKTQNAGHRLGGEAAAGVYAGSKSGRKPPGPSPSACCPSTTA

Target ORF information:

RefSeq Version	NM_001105945.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nanos C2HC-type zinc finger 3 (Nanos3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105945.1

ORF Insert Sequence:

ATGGGAACTT TTAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATGAGGAAG AGGAGCCTGA TGTCAGGCTG GACCCCAAAC CAGAGCCCAA GCCAAGCTCA 
GCAAGCCAGC AAGCCAGCAA GGAATCCTCA GCAGCTCCAG AACGCCTATG TTCATTCTGC 
AAACACAATG GCGAGTCTCG TGCGATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATCTT GCGAGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT CTGCCCACTC ACCGGCCAGG GCTATACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
AATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTG TCTATGCGGG TTCCAAAAGT 
GGCAGGAAGC CCCCTGGACC TTCACCCTCG GCCTGCTGTC CCTCCACTAC GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nanos3 ( XM_006255235.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255297.1 Rattus norvegicus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38686
Clone ID Related Accession (Same CDS sequence)	XM_006255235.3
Accession Version	XM_006255235.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	nanos homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255235.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACTT TTAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATGAGGAAG AGGAGCCTGA TGTCAGGCTG GACCCCAAAC CAGAGCCCAA GCCAAGCTCA 
GCAAGCCAGC AAGCCAGCAA GGAATCCTCA GCAGCTCCAG AACGCCTATG TTCATTCTGC 
AAACACAATG GCGAGTCTCG TGCGATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATCTT GCGAGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT CTGCCCACTC ACCGGCCAGG GCTATACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
AATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTT CCAAAAGTGG CAGGAAGCCC 
CCTGGACCTT CACCCTCGGC CTGCTGTCCC TCCACTACGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255297.1
CDS	370..894
Translation	MGTFNLWTDYLGLARLVGALHEEEEPDVRLDPKPEPKPSSASQQASKESSAAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATQEHAHTRRFCPLTGQGYTSVYCYTTRNSAGKKLTRPDKAKTQNAGHRLGGEAAAGSKSGRKPPGPSPSACCPSTTA

Target ORF information:

RefSeq Version	XM_006255235.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nanos C2HC-type zinc finger 3 (Nanos3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255235.3

ORF Insert Sequence:

ATGGGAACTT TTAATCTTTG GACAGATTAC CTAGGTCTGG CAAGACTGGT TGGGGCTCTG 
CATGAGGAAG AGGAGCCTGA TGTCAGGCTG GACCCCAAAC CAGAGCCCAA GCCAAGCTCA 
GCAAGCCAGC AAGCCAGCAA GGAATCCTCA GCAGCTCCAG AACGCCTATG TTCATTCTGC 
AAACACAATG GCGAGTCTCG TGCGATCTAT CAGTCCCATG TCCTGAAGGA TGAGGCAGGG 
CGGGTGCTGT GTCCCATCTT GCGAGATTAT GTGTGTCCCC AGTGTGGGGC CACTCAGGAG 
CATGCCCACA CTCGACGCTT CTGCCCACTC ACCGGCCAGG GCTATACTTC TGTCTACTGC 
TACACCACCC GGAATTCTGC AGGCAAAAAG CTGACCCGGC CTGACAAGGC AAAGACACAG 
AATGCTGGCC ACCGCCTTGG AGGAGAAGCA GCAGCAGGTT CCAAAAGTGG CAGGAAGCCC 
CCTGGACCTT CACCCTCGGC CTGCTGTCCC TCCACTACGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression.
Genes & development28(8)888-901(2014 Apr)
Bhandari D,Raisch T,Weichenrieder O,Jonas S,Izaurralde E

Analysis of meiosis regulators in human gonads: a sexually dimorphic spatio-temporal expression pattern suggests involvement of DMRT1 in meiotic entry.
Molecular human reproduction18(11)523-34(2012 Nov)
Jørgensen A,Nielsen JE,Blomberg Jensen M,Græm N,Rajpert-De Meyts E

NANOS3 function in human germ cell development.
Human molecular genetics20(11)2238-50(2011 Jun)
Julaton VT,Reijo Pera RA

Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3.
Reproduction (Cambridge, England)139(2)381-93(2010 Feb)
Yamaji M,Tanaka T,Shigeta M,Chuma S,Saga Y,Saitou M

Nanos3 maintains the germ cell lineage in the mouse by suppressing both Bax-dependent and -independent apoptotic pathways.
Developmental biology318(1)133-42(2008 Jun)
Suzuki H,Tsuda M,Kiso M,Saga Y