Afdn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Afdn
Entrez Gene ID	26955
Full Name	afadin, adherens junction formation factor
Synonyms	Af6,Mllt4
General protein information	Preferred Names afadin, adherens junction formation factor Names afadin myeloid/lymphoid or mixed lineage-leukemia translocation to 4 homolog myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 protein Af-6 translocated to, 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12
Summary	may play a role in linking the actin cytoskeleton to the plasma membrane [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013217.2	NP_037349.1	afadin

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	afd-1	NP_001021660.1
Gallus gallus (chicken)	MLLT4	XP_004935593.1
Danio rerio (zebrafish)	mllt4	XP_686655.5
Homo sapiens (human)	MLLT4	NP_001193937.1
Pan troglodytes (chimpanzee)	MLLT4	XP_518857.4
Macaca mulatta (Rhesus monkey)	MLLT4	XP_001083153.1
Canis lupus familiaris (dog)	MLLT4	XP_005615632.1
Bos taurus (cattle)	MLLT4	XP_005211198.1
Mus musculus (house mouse)	Mllt4	NP_034936.1
Rattus norvegicus (Norway rat)	Mllt4	NP_037349.1
Xenopus tropicalis (tropical clawed frog)	mllt4	XP_002931548.2

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic spatiotemporal patterns of alternative splicing of an F-actin scaffold protein, afadin, during murine development.
Matsuda T, Namura A, Oinuma I
Gene68956-68(2019 Mar)

Cyto-nuclear shuttling of afadin is required for rapid estradiol-mediated modifications of histone H3.
Sellers KJ, Watson IA, Gresz RE, Raval P, Srivastava DP
Neuropharmacology143153-162(2018 12)

A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to Promote α-Toxin Cytotoxicity.
Shah J, Rouaud F, Guerrera D, Vasileva E, Popov LM, Kelley WL, Rubinstein E, Carette JE, Amieva MR, Citi S
Cell reports25(8)2132-2147.e7(2018 11)

EphA2 proteomics in human keratinocytes reveals a novel association with afadin and epidermal tight junctions.
Perez White BE, Ventrella R, Kaplan N, Cable CJ, Thomas PM, Getsios S
Journal of cell science130(1)111-118(2017 01)

The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation.
Yamana S, Tokiyama A, Mizutani K, Hirata K, Takai Y, Rikitake Y
PloS one10(4)e0124259(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Afdn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afdn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42772	NM_013217.2 Latest version!	Rattus norvegicus afadin, adherens junction formation factor (Afdn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Afdn ( NM_013217.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037349.1 Rattus norvegicus afadin, adherens junction formation factor (Afdn), mRNA.

Price & Availability↑

CloneID	ORa42772
Clone ID Related Accession (Same CDS sequence)	NM_013217.2
Accession Version	NM_013217.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	afadin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U83230.1, CA511204.1, CK476731.1, EV767501.1, CN542111.1 and AA963026.1. On Aug 6, 2008 this sequence version replaced NM_013217.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT 
GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GCACAGCAAC CACTCAAGAT GTCATTGAAA CACTGGCAGA GAAGTTCCGC 
CCTGATATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA 
GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT 
GATCGGGAGG GTAGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT 
CAGAGTAATG GACCTGAGAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT 
CTGTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAGA AGGAGGCTTT GAGACAAGCC 
TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAATTCTCG TCTGGCTGCT 
GAAGTTTACA AAGACATGCC TGAAACCAGC TTTACTCGAA CGATTTCTAA TCCTGAAGTT 
GTTATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA 
GATGGGCGGC CCGACTCGGG TGGAACACTG AGAATCTATG CAGACAGTTT AAAACCAAAT 
ATTCCATACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTAGCAGAG 
TCCTTAGAGA AGTATGGTCT GGAAAAAGAG AACCCCAAGG ACTACTGCAT TGCTCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAT 
GACGATGAAT GCCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATTCCAAAGA AAATGAAGAA ACATGTTGAA 
GGCAAGCCAT TGAAGGGGAA AGATAGAGCT GACGGGTCTG GCTACGGCTC TGCTCTTCCT 
CCTGAGAAGC TGCCCTACTT AGTAGAGCTC AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC 
CTTCAGTTAA GTGTGACTGA AGTTGGGACA GAGAAGTTCG ATGACAATTC TATTCAGCTG 
TTTGGTCCTG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT 
GTGACACCCA GAAGTATGGA TGCAGAGACC TATGTGGATG GTCAGCGCAT CTCAGAGACC 
ACAATGCTAC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG 
GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG 
GGCCCAAGAC ATAAACCTGG AGCCGTTCAA GAGACAACCT TTGAATTGGG AGGAGATATC 
CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GATTGGACAG TGACAGGGTG 
TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CAATGATCCG ACTGGACCAG 
GAGCAGGACT ACCGGCGAAG AGAAAGCAGG ACTCAGGATG CTGCTGGGCC TGAACTGATG 
CTGCCTGCCA GCATTGAATT CCGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA 
AACTATACCA ATAGCTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG 
GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGGACA 
CATAAGGCCA TTGCTGTTGT GAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT CTGGATGGCA 
AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATTACTCTG 
GATGCCCAGG ATGTTCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCACTGC 
CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCCGA GGAGAACAGT 
CTGCAGAGAC CAAAAATAGA TGATGTGCTG CATACGCTCA CAGGAGCCAT GTCCTTGTTG 
CGGCGCTGCA GAGTCAATGC CGCCTTGACC ATCCAGCTGT TCTCTCAGCT TTTCCACTTC 
ATCAATATGT GGCTATTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGTTCCCAC 
TACTGGGGCG CAATTATCCG CCAGCAGCTG GGGCATATTG AAGCTTGGGC AGAGAAGCAG 
GGACTAGAGT TGGCAGCTGA CTGTCACTTG AGCAGGATCG TGCAGGCTAC TACGTTGCTT 
ACAATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA 
AATTCTCTGC AGCTTCAAGC CCTCTTACAG AATTACCACT GTGCACCTGA TGAGCCTTTT 
ATTCCTACGG ATCTCATAGA AAATGTCGTG GCTGTTGCTG AGAACACAGC TGATGAGCTT 
GCCCGCAGTG ATGGCAGAGA TGTGCAGTTA GAGGAGGACC CTGATCTACA ACTGCCCTTT 
CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TGGTTCCTCA CACACGTTCT 
CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT CATGCGTGAG 
AACACAGAAC TGACCCAGCC TCTGAGGAAG GAGCCTGAGG TAATCACTGT GACCCTGAAG 
AAGCAGAATG GAATGGGCCT CAGCATTGTT GCAGCAAAGG GTGCTGGCCA GGATAAACTG 
GGAATCTACG TTAAGTCCGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCT 
GCTGGTGACC AACTCCTTAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTAACAC TGGAAGTTGC AAAGCAAGGC 
GCTATCTATC ACGGACTGGC CACTCTCCTT AACCAGCCAT CACCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGCG AAGGTTTTGA GCTCTATAAT 
AATTCAGCTC AAAACGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG 
CCAAAGAAGC TGCCTGGTGA CGACAGGCTG ATGAAAAACA GAGCTGACCA CCGTTCCAGC 
CCCAATGTGG CAAATCAGCC CCCTAGTCCT GGAGGGAAGA GTCCATATAC CTCTGGAACA 
GCAGCTAAAA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCA 
CCTAGACCTG AAGCCTATCC TATCCCTACT CAGACATATA CCAGAGAGTA TTTTACCTTT 
CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTGTTCAAA ACCAGTGGCC AAATTATGAG 
GAAAAACCAC ACATGCACAC AGAAAGTGAT CATGCCAGTA TTGCAATCCA GCGTGTTACC 
CGTTCCCAGG AAGAGCTTCG GGAAGAGAAG GTTTACCAAC TTGAGCGTCA TCGAGTAGAG 
TCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA 
TCTAGCACGT CTAGCCAGGA GCACCTGAAC CATTCCTCCA AGTCAGTTAC CCCTGCTTCC 
ACTCTGACCA AGAGTGGTCC TGGTCGTTGG AAAACCCCAG CAGCTGTGCT CCCCACCCCT 
GTGGCTGTCT CCCAACCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCACCTGCC 
CACTACACCA GTGATTTTGA TGGTATTTCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC 
AACCAAGCAG CTCCCCAGTC TGCTCAGGTG GCTGCTGCAG AGAGGAAAAA GAGAGAGGAG 
CATCAGAGGT GGTATGAAAA AGAGAAGGCC CGCCTGGAGG AGGAACGAGA GAGAAAGCGC 
AGGGAGCAAG AGAGGAAGTT GGGGCAGATG CGCACTCAGT CCCTGAACCC TGCTTCCTTC 
TCTCCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGTA CTATTGAGCG CAGGGATCTG 
CAGTACATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGGG ATAGTCTGTC TCCAGACCCA 
TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTAGACATG 
CTAAGCAAGG AGATCCATGA GCTGCAAAAC AAGGGGGACC GTACTGCAGA GGAGAGTGAC 
CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAGCAG 
AAGGATGAGG ATGATGATGA AGAGGAAGAT GATGATGTTG ACACCATGCT GATCATGCAG 
CGACTGGAGG CGGAGCGGAG AGCCAGGTTG CAGGATGAAG AGCGCAGGCG CCAGCAACAG 
TTGGAAGAGA TGCGGAAACG AGAAGTTGAA GACAGAGTAA GACAAGAGGA AGATGGACGC 
CATCAGGAGG AGGAGCGAGT AAAGAGAGAT GCTGAAGAAA AGAGGCGACA GGAAGAAGGG 
TATTACAGCC GCCTAGAAGC TGAGAGGCGC AGACAGCATG AAGAGGCAGC ACGCAGGTTG 
CTGGAGCCCG AAGAACCCGG GCTGAGCCGA CCTCCACTTC CACAGGACTA CGAGCCCCCA 
TCCCAGTCCT CAGCACCCAG TGCCCCTCCT CCCCCACCTC AGCGAAATGC CTCCTACCTC 
AAAACACAGG TCCTCTCCCC AGACTCGCTG TTCACTGCCA AGTTTGTTGC GTATGATGAT 
GATGATGAGG AGGAGAACTA CGTCCCAGCA GGACCAAACT CTTATTCGGG ATCTGCTGGC 
ACAACTGCTG GAACTTATGA TGCTCCTCGG GACACAAGAG AAAAACTCAG TAGAAGCCAA 
GATGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAACC TGACATTTAG AGAGCGTCAG 
CGCCTCTTTT CACAAGGTCA AGATGTGTCA GACAAAGTGA AAGCTTCTCG TAAATTAACA 
GAGCTTGAGA ATGAACTCAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037349.1
CDS	326..5815
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKPLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQFGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDIHSGTALPASRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQDYRRRESRTQDAAGPELMLPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENTELTQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSPYTSGTAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPVQNQWPNYEEKPHMHTESDHASIAIQRVTRSQEELREEKVYQLERHRVESGMDRKCDSDMWINQSSSVESSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPAHYTSDFDGISMDLPLPPPPANQAAPQSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKGDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREVEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPGLSRPPLPQDYEPPSQSSAPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDDDDEEENYVPAGPNSYSGSAGTTAGTYDAPRDTREKLSRSQDADLPGSSGAPENLTFRERQRLFSQGQDVSDKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	NM_013217.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus afadin, adherens junction formation factor (Afdn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013217.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAAGAGCGG CGGAAGCTGG CCGACATCAT TCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTTCAT 
GGAGTCATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GCACAGCAAC CACTCAAGAT GTCATTGAAA CACTGGCAGA GAAGTTCCGC 
CCTGATATGC GCATGCTCTC CTCTCCCAAG TACTCCCTCT ATGAAGTGCA TGTCAGTGGA 
GAAAGAAGAT TGGACATTGA TGAGAAACCT CTAGTTGTAC AGTTGAATTG GAACAAAGAT 
GATCGGGAGG GTAGATTCGT CCTTAAGAAC GAGAATGACG CCATTCCTGC CAAGAAGGCT 
CAGAGTAATG GACCTGAGAA GCAGGAGAAA GAAGGTGTTA TTCAGAACTT CAAAAGAACT 
CTGTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAGA AGGAGGCTTT GAGACAAGCC 
TCTGATAAGG AAGAAAGGCC TTCTCAAGGG GATGACAGTG AGAATTCTCG TCTGGCTGCT 
GAAGTTTACA AAGACATGCC TGAAACCAGC TTTACTCGAA CGATTTCTAA TCCTGAAGTT 
GTTATGAAAC GGCGGCGGCA GCAAAAACTG GAGAAGAGAA TGCAAGAGTT TCGGAGCTCA 
GATGGGCGGC CCGACTCGGG TGGAACACTG AGAATCTATG CAGACAGTTT AAAACCAAAT 
ATTCCATACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTAGCAGAG 
TCCTTAGAGA AGTATGGTCT GGAAAAAGAG AACCCCAAGG ACTACTGCAT TGCTCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAGAGAGGTG CTAAGGAAAT AATTCTTGAT 
GACGATGAAT GCCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAGTTGA AGAGGAGACC ACCGGACTAC ATTCCAAAGA AAATGAAGAA ACATGTTGAA 
GGCAAGCCAT TGAAGGGGAA AGATAGAGCT GACGGGTCTG GCTACGGCTC TGCTCTTCCT 
CCTGAGAAGC TGCCCTACTT AGTAGAGCTC AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGCTCT GACTCCAGAG ACAAGCCAAA GTTATACCGC 
CTTCAGTTAA GTGTGACTGA AGTTGGGACA GAGAAGTTCG ATGACAATTC TATTCAGCTG 
TTTGGTCCTG GAATTCAGCC CCATCACTGT GATCTCACCA ACATGGATGG AGTGGTCACT 
GTGACACCCA GAAGTATGGA TGCAGAGACC TATGTGGATG GTCAGCGCAT CTCAGAGACC 
ACAATGCTAC AGAGTGGCAT GAGACTGCAG TTTGGCACCT CGCATGTGTT TAAGTTTGTG 
GACCCCATCC AGGACCACGT TCTTTCCAAG AGATCTGTGG ATGGAGGCCT GATGGTCAAG 
GGCCCAAGAC ATAAACCTGG AGCCGTTCAA GAGACAACCT TTGAATTGGG AGGAGATATC 
CACAGTGGGA CAGCATTGCC GGCAAGCAGG AGCACTACTA GATTGGACAG TGACAGGGTG 
TCCTCTGCCT CTAGCACAGC TGAGCGAGGG ATGGTGAAGC CAATGATCCG ACTGGACCAG 
GAGCAGGACT ACCGGCGAAG AGAAAGCAGG ACTCAGGATG CTGCTGGGCC TGAACTGATG 
CTGCCTGCCA GCATTGAATT CCGGGAAAGC TCTGAAGACT CTTTTTTATC TGCCATTATA 
AACTATACCA ATAGCTCTAC AGTTCATTTT AAGTTGTCAC CTACATATGT GTTATACATG 
GCATGCCGGT ATGTATTGTC CAGCCAGCAC AGGCCCGACA TCAGTCCTAC AGAGCGGACA 
CATAAGGCCA TTGCTGTTGT GAACAAGATG GTGAGCATGA TGGAAGGGGT CATCCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAC ATTGCAGGAG CACTTGCTTT CTGGATGGCA 
AATGCATCTG AGCTTCTCAA CTTCATCAAG CAGGACCGAG ACCTTAGTCG GATTACTCTG 
GATGCCCAGG ATGTTCTAGC ACACTTGGTT CAGATGGCAT TTAAGTACTT GGTTCACTGC 
CTTCAGTCAG AACTTAATAA CTACATGCCA GCCTTTCTGG ATGATCCCGA GGAGAACAGT 
CTGCAGAGAC CAAAAATAGA TGATGTGCTG CATACGCTCA CAGGAGCCAT GTCCTTGTTG 
CGGCGCTGCA GAGTCAATGC CGCCTTGACC ATCCAGCTGT TCTCTCAGCT TTTCCACTTC 
ATCAATATGT GGCTATTCAA CAGACTGGTG ACTGACCCAG ATTCAGGCCT GTGTTCCCAC 
TACTGGGGCG CAATTATCCG CCAGCAGCTG GGGCATATTG AAGCTTGGGC AGAGAAGCAG 
GGACTAGAGT TGGCAGCTGA CTGTCACTTG AGCAGGATCG TGCAGGCTAC TACGTTGCTT 
ACAATGGATA AGTATGTACC TGATGACATT CCAAATATAA ACAGCACATG CTTTAAGTTA 
AATTCTCTGC AGCTTCAAGC CCTCTTACAG AATTACCACT GTGCACCTGA TGAGCCTTTT 
ATTCCTACGG ATCTCATAGA AAATGTCGTG GCTGTTGCTG AGAACACAGC TGATGAGCTT 
GCCCGCAGTG ATGGCAGAGA TGTGCAGTTA GAGGAGGACC CTGATCTACA ACTGCCCTTT 
CTTCTGCCAG AAGATGGCTA TTCCTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TGGTTCCTCA CACACGTTCT 
CCAGGTACCT GGACAATATA TTTTGAAGGA GCAGATTATG AAAGTCACCT CATGCGTGAG 
AACACAGAAC TGACCCAGCC TCTGAGGAAG GAGCCTGAGG TAATCACTGT GACCCTGAAG 
AAGCAGAATG GAATGGGCCT CAGCATTGTT GCAGCAAAGG GTGCTGGCCA GGATAAACTG 
GGAATCTACG TTAAGTCCGT TGTCAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCT 
GCTGGTGACC AACTCCTTAG TGTGGATGGA CGAAGTCTGG TTGGACTTTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACCAGTTCC GTGGTAACAC TGGAAGTTGC AAAGCAAGGC 
GCTATCTATC ACGGACTGGC CACTCTCCTT AACCAGCCAT CACCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCAGA CCAAAGAGCG AAGGTTTTGA GCTCTATAAT 
AATTCAGCTC AAAACGGCTC CCCAGAGAGT CCTCAGATGC CTTGGACAGA GTACAGTGAG 
CCAAAGAAGC TGCCTGGTGA CGACAGGCTG ATGAAAAACA GAGCTGACCA CCGTTCCAGC 
CCCAATGTGG CAAATCAGCC CCCTAGTCCT GGAGGGAAGA GTCCATATAC CTCTGGAACA 
GCAGCTAAAA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCA 
CCTAGACCTG AAGCCTATCC TATCCCTACT CAGACATATA CCAGAGAGTA TTTTACCTTT 
CCAGCTTCAA AGTCGCAGGA TCGGATGGCT CCTGTTCAAA ACCAGTGGCC AAATTATGAG 
GAAAAACCAC ACATGCACAC AGAAAGTGAT CATGCCAGTA TTGCAATCCA GCGTGTTACC 
CGTTCCCAGG AAGAGCTTCG GGAAGAGAAG GTTTACCAAC TTGAGCGTCA TCGAGTAGAG 
TCAGGCATGG ATCGCAAGTG TGACAGTGAT ATGTGGATCA ATCAGAGCTC CTCAGTGGAA 
TCTAGCACGT CTAGCCAGGA GCACCTGAAC CATTCCTCCA AGTCAGTTAC CCCTGCTTCC 
ACTCTGACCA AGAGTGGTCC TGGTCGTTGG AAAACCCCAG CAGCTGTGCT CCCCACCCCT 
GTGGCTGTCT CCCAACCCAT TCGAACAGAC CTCCCTCCAC CACCACCTCC ACCACCTGCC 
CACTACACCA GTGATTTTGA TGGTATTTCA ATGGATTTGC CCCTCCCACC TCCTCCTGCC 
AACCAAGCAG CTCCCCAGTC TGCTCAGGTG GCTGCTGCAG AGAGGAAAAA GAGAGAGGAG 
CATCAGAGGT GGTATGAAAA AGAGAAGGCC CGCCTGGAGG AGGAACGAGA GAGAAAGCGC 
AGGGAGCAAG AGAGGAAGTT GGGGCAGATG CGCACTCAGT CCCTGAACCC TGCTTCCTTC 
TCTCCTTTGG CCACACAGGC CAAGCCAGAA AAGCCTTCCA CACTGCAGAG GCCCCAAGAA 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGTA CTATTGAGCG CAGGGATCTG 
CAGTACATAA CCATCAGTAA AGAGGAGCTG TCCTCTGGGG ATAGTCTGTC TCCAGACCCA 
TGGAAACGAG ATGCCAGGGA GAAGCTGGAG AAGCAACAGC AAATGCACAT TGTAGACATG 
CTAAGCAAGG AGATCCATGA GCTGCAAAAC AAGGGGGACC GTACTGCAGA GGAGAGTGAC 
CGCCTGCGGA AGCTTATGCT GGAGTGGCAG TTCCAGAAGA GACTACAGGA GTCCAAGCAG 
AAGGATGAGG ATGATGATGA AGAGGAAGAT GATGATGTTG ACACCATGCT GATCATGCAG 
CGACTGGAGG CGGAGCGGAG AGCCAGGTTG CAGGATGAAG AGCGCAGGCG CCAGCAACAG 
TTGGAAGAGA TGCGGAAACG AGAAGTTGAA GACAGAGTAA GACAAGAGGA AGATGGACGC 
CATCAGGAGG AGGAGCGAGT AAAGAGAGAT GCTGAAGAAA AGAGGCGACA GGAAGAAGGG 
TATTACAGCC GCCTAGAAGC TGAGAGGCGC AGACAGCATG AAGAGGCAGC ACGCAGGTTG 
CTGGAGCCCG AAGAACCCGG GCTGAGCCGA CCTCCACTTC CACAGGACTA CGAGCCCCCA 
TCCCAGTCCT CAGCACCCAG TGCCCCTCCT CCCCCACCTC AGCGAAATGC CTCCTACCTC 
AAAACACAGG TCCTCTCCCC AGACTCGCTG TTCACTGCCA AGTTTGTTGC GTATGATGAT 
GATGATGAGG AGGAGAACTA CGTCCCAGCA GGACCAAACT CTTATTCGGG ATCTGCTGGC 
ACAACTGCTG GAACTTATGA TGCTCCTCGG GACACAAGAG AAAAACTCAG TAGAAGCCAA 
GATGCAGACT TACCTGGCAG TTCTGGAGCC CCTGAAAACC TGACATTTAG AGAGCGTCAG 
CGCCTCTTTT CACAAGGTCA AGATGTGTCA GACAAAGTGA AAGCTTCTCG TAAATTAACA 
GAGCTTGAGA ATGAACTCAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic spatiotemporal patterns of alternative splicing of an F-actin scaffold protein, afadin, during murine development.
Gene68956-68(2019 Mar)
Matsuda T,Namura A,Oinuma I

Cyto-nuclear shuttling of afadin is required for rapid estradiol-mediated modifications of histone H3.
Neuropharmacology143153-162(2018 12)
Sellers KJ,Watson IA,Gresz RE,Raval P,Srivastava DP

A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to Promote α-Toxin Cytotoxicity.
Cell reports25(8)2132-2147.e7(2018 11)
Shah J,Rouaud F,Guerrera D,Vasileva E,Popov LM,Kelley WL,Rubinstein E,Carette JE,Amieva MR,Citi S

EphA2 proteomics in human keratinocytes reveals a novel association with afadin and epidermal tight junctions.
Journal of cell science130(1)111-118(2017 01)
Perez White BE,Ventrella R,Kaplan N,Cable CJ,Thomas PM,Getsios S

The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation.
PloS one10(4)e0124259(2015)
Yamana S,Tokiyama A,Mizutani K,Hirata K,Takai Y,Rikitake Y

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Afdn cDNA ORF clone, Rattus norvegicus(Norway rat)

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