Rasa1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasa1
Entrez Gene ID	25676
Full Name	RAS p21 protein activator 1
Synonyms	GAPX,Rasa
General protein information	Preferred Names RAS p21 protein activator 1 Names ras GTPase-activating protein 1 GAP GTPase-activating protein RAS p21 protein activator (GTPase activating protein) 1 p120GAP rasGAP
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q11
Summary	may play a role in Ras GTPase mediated signal transduction; may be involved in cell proliferation and cell migration [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013135.1	NP_037267.1	ras GTPase-activating protein 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	RASA1	XP_001084074.2
Xenopus tropicalis (tropical clawed frog)	LOC100135092	NP_001107303.1
Pan troglodytes (chimpanzee)	RASA1	XP_517663.3
Canis lupus familiaris (dog)	RASA1	XP_536302.3
Bos taurus (cattle)	RASA1	NP_776874.1
Mus musculus (house mouse)	Rasa1	NP_663427.2
Rattus norvegicus (Norway rat)	Rasa1	NP_037267.1
Gallus gallus (chicken)	RASA1	XP_424907.4
Danio rerio (zebrafish)	rasa1a	XP_001342009.2
Drosophila melanogaster (fruit fly)	vap	NP_727926.2
Homo sapiens (human)	RASA1	NP_002881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002729	XP_312197.3

Gene Ontology
Bibliography

Related articles in PubMed

Sex- and estrogen-dependent regulation of a miRNA network in the healthy and hypertrophied heart.
Queirós AM, Eschen C, Fliegner D, Kararigas G, Dworatzek E, Westphal C, Sanchez Ruderisch H, Regitz-Zagrosek V
International journal of cardiology169(5)331-8(2013 Nov)

A novel RASA1 mutation causing capillary malformation-arteriovenous malformation (CM-AVM) presenting during pregnancy.
Durrington HJ, Firth HV, Patient C, Belham M, Jayne D, Burrows N, Morrell NW, Chilvers ER
American journal of medical genetics. Part A161A(7)1690-4(2013 Jul)

miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.
Wang J, Ruan K
Biochemical and biophysical research communications402(2)222-7(2010 Nov)

Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1.
Mehlitz A, Banhart S, Mäurer AP, Kaushansky A, Gordus AG, Zielecki J, Macbeath G, Meyer TF
The Journal of cell biology190(1)143-57(2010 Jul)

Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, Walicki J, Abderrahmani A, Cornu M, Waeber G, Thorens B, Widmann C
The Journal of biological chemistry280(38)32835-42(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rasa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02104	NM_013135.1 Latest version!	Rattus norvegicus RAS p21 protein activator 1 (Rasa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasa1 ( NM_013135.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037267.1 Rattus norvegicus RAS p21 protein activator 1 (Rasa1), mRNA.

Price & Availability↑

CloneID	ORa02104
Clone ID Related Accession (Same CDS sequence)	NM_013135.1
Accession Version	NM_013135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Rattus norvegicus(Norway rat)
Product	ras GTPase-activating protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L13151.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCGG CGACCGCGGG GACCGGCGGC 
GCCGCGGCCA CGGGCTCCAG CGCCTACCCA GCAGCGTGCC GGGTAAAGTT ACCCGCGGCC 
CCTCCGATGG CAGTCGCCCC GTGTCCTGGG CTGGCGGACA CCGACCTGGC TGCGGCCCTC 
GGTGGGGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GGCCGGTGTC TGGTGTCCTG 
GGGGGCGCCG CGCTGACCGG CGGTGCTGCA GCTGGCGTGG CGGGTGCTGC AGCCGCTGGA 
CCTGCCGGAG ACATCGCTCT CACCAAGGGG ACTCTCTCTC TGCCTGCTGA GACTCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCT CCTCCTCTGT TGCCTCCTTT GGGCTCCGGT 
CTGGGGACAG TGGATGAAGG TGACTCCTTG GATGGACCAG AATACGAGGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCAACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTACCTTAT AAGAGAGAGT 
GATCGGAGGC CAGGGTCCTT TGTACTTTCA TTTCTTAGCC AGACAAATGT TGTCAATCAT 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGTTT TTCTTCACTC 
TCAGACCTAA TAGGATATTA CAGTCACGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TACCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGAC GTGTACGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTCTTAA AGGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACAAACC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AGGACCTAGT GGAAGAGGTG GGCCGAGAAG AAGATCCACA TGAAGGCAAA 
ATATGGTTCC ATGGGAAGAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGC 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCCCTGTAT 
TTTCGGACCA ATGAAAATAT CCAGCGATTT AAAATTTGTC CAACACCTAA CAATCAGTTC 
ATGATGGGAG GCCGCTATTA TAACAGTATT GGGGACATCA TAGACCACTA TCGGAAAGAA 
CAGATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGACAAGTA 
CTCAATGACA CTGTGGATGG GAAGGAAATC TATAATACAA TTCGTCGTAA AACAAAGGAT 
GCATTTTATA AAAATATTGT TAAAAAAGGT TATCTTCTGA AGAAGGGCAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAGGGC AGTGATGCCC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGATCTCA GTGTATGTTC TGTCTACGTT 
GTCCATGATA GTCTCTTCGG CAGGCCAAAC TGTTTTCAGA TAGTCGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGCCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTATTACAT ATTGAAGAAG CTCATAAACT ACCAGTAAAA 
CACTTCACTA ATCCATATTG TAACATCTAC TTGAATAGTG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGTAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCCAC AGATGAATGG 
TTTCTGCTAA GTTCTCATAT ACCACTAAAA GGTATTGAAC CAGGATCTCT GCGTGTTCGA 
GCACGATACT CTATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAGA AGGAACTTCA CGTAGTCTAT GCTCTATCAC ACGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTGAAAATA TTTCTTCATG AAAAGCTTGA GTCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCGAGTAC CTTGATGGAG CAGTACATGA AAGCCACGGC CACGCAGTTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATAATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCAT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TTTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CACGAGTTGT TAGTGGTTTT GTTTTTCTTC GGCTTATCTG CCCAGCTATC 
CTGAATCCAC GGATGTTTAA TATCATCTCA GATTCTCCAT CTCCTATTGC TGCAAGAACA 
CTGACATTAG TGGCTAAATC TGTGCAGAAT TTAGCAAATC TTGTGGAATT TGGAGCTAAG 
GAGCCTTATA TGGAAGGTGT CAATCCATTC ATCAAAAGTA ATAAACATCG CATGATCATG 
TTTTTAGATG AACTTGGGAA TGTACCTGAG CTTCCGGACA CTACAGAACA TTCTAGAACT 
GACCTGTCTC GTGACTTAGC AGCATTGCAT GAGATTTGTG TAGCTCATTC AGATGAACTG 
AGAACACTCA GTAATGAGCG TGGTGTACAG CAGCATGTAC TGAAAAAGCT TCTGGCAATA 
ACAGAACTGC TTCAACAAAA GCAAAACCAG TATACAAAAA CCAATGATGT CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037267.1
CDS	1..3117
Translation	MMAAEAGSEEGGPATAGTGGAAATGSSAYPAACRVKLPAAPPMAVAPCPGLADTDLAAALGGGAASGSGFLGTGPVSGVLGGAALTGGAAAGVAGAAAAGPAGDIALTKGTLSLPAETLGPGGGFPPLPPPPLLPPLGSGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLLSILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGVQQHVLKKLLAITELLQQKQNQYTKTNDVR

Target ORF information:

RefSeq Version	NM_013135.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAS p21 protein activator 1 (Rasa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013135.1

ORF Insert Sequence:

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCGG CGACCGCGGG GACCGGCGGC 
GCCGCGGCCA CGGGCTCCAG CGCCTACCCA GCAGCGTGCC GGGTAAAGTT ACCCGCGGCC 
CCTCCGATGG CAGTCGCCCC GTGTCCTGGG CTGGCGGACA CCGACCTGGC TGCGGCCCTC 
GGTGGGGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GGCCGGTGTC TGGTGTCCTG 
GGGGGCGCCG CGCTGACCGG CGGTGCTGCA GCTGGCGTGG CGGGTGCTGC AGCCGCTGGA 
CCTGCCGGAG ACATCGCTCT CACCAAGGGG ACTCTCTCTC TGCCTGCTGA GACTCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCT CCTCCTCTGT TGCCTCCTTT GGGCTCCGGT 
CTGGGGACAG TGGATGAAGG TGACTCCTTG GATGGACCAG AATACGAGGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCAACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTACCTTAT AAGAGAGAGT 
GATCGGAGGC CAGGGTCCTT TGTACTTTCA TTTCTTAGCC AGACAAATGT TGTCAATCAT 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGTTT TTCTTCACTC 
TCAGACCTAA TAGGATATTA CAGTCACGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TACCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGAC GTGTACGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTCTTAA AGGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACAAACC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AGGACCTAGT GGAAGAGGTG GGCCGAGAAG AAGATCCACA TGAAGGCAAA 
ATATGGTTCC ATGGGAAGAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGC 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCCCTGTAT 
TTTCGGACCA ATGAAAATAT CCAGCGATTT AAAATTTGTC CAACACCTAA CAATCAGTTC 
ATGATGGGAG GCCGCTATTA TAACAGTATT GGGGACATCA TAGACCACTA TCGGAAAGAA 
CAGATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGACAAGTA 
CTCAATGACA CTGTGGATGG GAAGGAAATC TATAATACAA TTCGTCGTAA AACAAAGGAT 
GCATTTTATA AAAATATTGT TAAAAAAGGT TATCTTCTGA AGAAGGGCAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAGGGC AGTGATGCCC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGATCTCA GTGTATGTTC TGTCTACGTT 
GTCCATGATA GTCTCTTCGG CAGGCCAAAC TGTTTTCAGA TAGTCGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGCCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTATTACAT ATTGAAGAAG CTCATAAACT ACCAGTAAAA 
CACTTCACTA ATCCATATTG TAACATCTAC TTGAATAGTG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGTAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCCAC AGATGAATGG 
TTTCTGCTAA GTTCTCATAT ACCACTAAAA GGTATTGAAC CAGGATCTCT GCGTGTTCGA 
GCACGATACT CTATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAGA AGGAACTTCA CGTAGTCTAT GCTCTATCAC ACGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTGAAAATA TTTCTTCATG AAAAGCTTGA GTCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCGAGTAC CTTGATGGAG CAGTACATGA AAGCCACGGC CACGCAGTTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATAATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCAT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TTTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CACGAGTTGT TAGTGGTTTT GTTTTTCTTC GGCTTATCTG CCCAGCTATC 
CTGAATCCAC GGATGTTTAA TATCATCTCA GATTCTCCAT CTCCTATTGC TGCAAGAACA 
CTGACATTAG TGGCTAAATC TGTGCAGAAT TTAGCAAATC TTGTGGAATT TGGAGCTAAG 
GAGCCTTATA TGGAAGGTGT CAATCCATTC ATCAAAAGTA ATAAACATCG CATGATCATG 
TTTTTAGATG AACTTGGGAA TGTACCTGAG CTTCCGGACA CTACAGAACA TTCTAGAACT 
GACCTGTCTC GTGACTTAGC AGCATTGCAT GAGATTTGTG TAGCTCATTC AGATGAACTG 
AGAACACTCA GTAATGAGCG TGGTGTACAG CAGCATGTAC TGAAAAAGCT TCTGGCAATA 
ACAGAACTGC TTCAACAAAA GCAAAACCAG TATACAAAAA CCAATGATGT CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sex- and estrogen-dependent regulation of a miRNA network in the healthy and hypertrophied heart.
International journal of cardiology169(5)331-8(2013 Nov)
Queirós AM,Eschen C,Fliegner D,Kararigas G,Dworatzek E,Westphal C,Sanchez Ruderisch H,Regitz-Zagrosek V

A novel RASA1 mutation causing capillary malformation-arteriovenous malformation (CM-AVM) presenting during pregnancy.
American journal of medical genetics. Part A161A(7)1690-4(2013 Jul)
Durrington HJ,Firth HV,Patient C,Belham M,Jayne D,Burrows N,Morrell NW,Chilvers ER

miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.
Biochemical and biophysical research communications402(2)222-7(2010 Nov)
Wang J,Ruan K

Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1.
The Journal of cell biology190(1)143-57(2010 Jul)
Mehlitz A,Banhart S,Mäurer AP,Kaushansky A,Gordus AG,Zielecki J,Macbeath G,Meyer TF

Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
The Journal of biological chemistry280(38)32835-42(2005 Sep)
Yang JY,Walicki J,Abderrahmani A,Cornu M,Waeber G,Thorens B,Widmann C

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Rasa1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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