Dgkg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgkg
Entrez Gene ID	25666
Full Name	diacylglycerol kinase, gamma
Synonyms	DGKIII,Dagk3
General protein information	Preferred Names diacylglycerol kinase, gamma Names diacylglycerol kinase gamma 88 kDa diacylglycerol kinase DAG kinase gamma DGK-gamma Diacylglycerol kinase 3 (gamma) diglyceride kinase gamma
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	putative diacylglycerol kinase associated with the cytoskeleton of purkinje cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006248540.3	XP_006248602.1	diacylglycerol kinase gamma isoform X3
XM_006248538.3	XP_006248600.1	diacylglycerol kinase gamma isoform X1
XM_006248539.3	XP_006248601.1	diacylglycerol kinase gamma isoform X2
NM_013126.1	NP_037258.1	diacylglycerol kinase gamma

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DGKG	XP_545239.2
Rattus norvegicus (Norway rat)	Dgkg	NP_037258.1
Pan troglodytes (chimpanzee)	DGKG	XP_001152821.1
Macaca mulatta (Rhesus monkey)	DGKG	XP_001092912.1
Danio rerio (zebrafish)	LOC567728	XP_696120.6
Homo sapiens (human)	DGKG	NP_001337.2
Bos taurus (cattle)	DGKG	XP_002684915.2
Mus musculus (house mouse)	Dgkg	NP_619591.1
Gallus gallus (chicken)	DGKG	XP_422650.4
Oryza sativa (rice)	Os03g0425300	NP_001173480.1

Gene Ontology
Bibliography

Related articles in PubMed

Diacylglycerol kinase γ regulates antigen-induced mast cell degranulation by mediating Ca(2+) influxes.
Sakuma M, Shirai Y, Ueyama T, Saito N
Biochemical and biophysical research communications445(2)340-5(2014 Mar)

Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.
Gellett AM, Kharel Y, Sunkara M, Morris AJ, Lynch KR
Biochemical and biophysical research communications422(4)758-63(2012 Jun)

Phosphorylation and up-regulation of diacylglycerol kinase gamma via its interaction with protein kinase C gamma.
Yamaguchi Y, Shirai Y, Matsubara T, Sanse K, Kuriyama M, Oshiro N, Yoshino K, Yonezawa K, Ono Y, Saito N
The Journal of biological chemistry281(42)31627-37(2006 Oct)

Immunocytochemical localization of a neuron-specific diacylglycerol kinase beta and gamma in the developing rat brain.
Adachi N, Oyasu M, Taniguchi T, Yamaguchi Y, Takenaka R, Shirai Y, Saito N
Brain research. Molecular brain research139(2)288-99(2005 Oct)

High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence.
Kwitek AE, Gullings-Handley J, Yu J, Carlos DC, Orlebeke K, Nie J, Eckert J, Lemke A, Andrae JW, Bromberg S, Pasko D, Chen D, Scheetz TE, Casavant TL, Soares MB, Sheffield VC, Tonellato PJ, Jacob HJ
Genome research14(4)750-7(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dgkg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgkg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02075	NM_013126.1 Latest version!	Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa34398	XM_006248538.3 Latest version!	Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa34399	XM_006248539.3 Latest version!	Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa34400	XM_006248540.3 Latest version!	Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgkg ( NM_013126.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037258.1 Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), mRNA.

Price & Availability↑

CloneID	ORa02075
Clone ID Related Accession (Same CDS sequence)	NM_013126.1
Accession Version	NM_013126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase gamma
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D38448.1. On Jan 12, 2005 this sequence version replaced XM_346420.1. ##Evidence-Data-START## Transcript exon combination :: D38448.1, FQ231209.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGACG GGCAATGGGT CTGTCTCTCA TCAGAAGAAT TTGATCAACT TCAGAAATAC 
TCTGAATATT CCTCCAAGAA GATAAAAGAT GTGTTGGCTG AGTTTAATGA AGGTGGAAGC 
CTCAAGCAAT ATGACCCACA CAAGCCCATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCATACCTGG AGGTGGATCT TCCTCAGCCA CTGAGCACAA ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACAAGAGAC ACCTGACCAC CCAAAGGAGG GGGCCAGCAG TAGTGAGCCG 
AATGTCTCAG ATTCTAATGC GGAGAGCACT GCCAAAGCGG ATGCTGCCTG CGCTCCAGAC 
ACTGAATCAA AACCGATCAA GACACAGGTA CCGTCTGAAG AACTAGAGGC TGCAGCCCCA 
TGGGGAGAAC CTAACGCTCC GGCTTCAAGC TCCGATGCCC CCATAGTGTA CCTGAAGGAT 
GTGGTGTGTT ACTTGTCCCT GATGGAGACC GGGAGGCCTC AGGATAAACT AGAATTCATG 
TTTCGTCTCT ATGACTCGGA TGAAAATGAA CTCCTGGACC AAGCGGAGCT GGATCAAATT 
GTGAGCCAAA TGCTACATGT TGCCCAGTAC CTGGAGTGGG ATCCCACAGA GCTCAGACCT 
ATACTGAAGG AGATGTTGCA AGGCATGGAC TACAACAAGG ATGGCTTCGT GTCTCTAGAG 
GAGTGGGTCA GTGGGGGGAT GACCACCATC CCGTTGCTGG TCCTCCTGGG AATGGATGAC 
TCCGCCTCCA AGGGGGATGG GAGGCACGCC TGGACCCTGA AGCACTTCAA GAAGCCCACT 
TACTGCAACT TCTGCCACAT CATGCTGATG GGCGTTCGGA AGCAAGGCCT GTGCTGCATC 
TACTGTAAAT ATGCTGTCCA CCAACGCTGT GTATCCAACA GCATTCCTGG ATGTGTGAAA 
ACATACTCCA AAGCCAAAAG GAGCGGTGAG GTGATGCAGC ACGCGTGGGT GGAAGGGAAC 
TCCTCCGTCA AGTGTGACCG GTGCCACAAA AGTATCAAGT GCTACCAGAG TGTCACTGCG 
CGGCACTGCG TGTGGTGCCG GATGACGTTT CACCGCAAAT GTGAATTGTC GACAGCGTGC 
GATGGTGGGG AACTCAAAGA CCACATTTTG CTGCCTACCT CCATATACCC TGTCACCCGA 
GACCGGCAAG CAGGGAAGTC CGATAGCGGT GCGGCGGCCA AGGGTGAACT TGTAATGCAG 
TATAAGATCA TCCCAAGTCC GGGGACGCAT CCCCTGCTGG TCCTGGTGAA CCCGAAGAGT 
GGAGGAAGAC AAGGAGAAAG AATTCTTCAG AAATTCCACT ACCTGCTCAA CCCCAAACAA 
GTTTTCAACC TGGACAAAGG GGGGCCTACG CCAGGATTGA ACTTCTTTCA GGATACCCCG 
GACTTCCGTG TTCTTGCCTG TGGCGGAGAT GGGACAGTTG GCTGGATTTT GGATTGCATT 
GACAAGGCCA ACTTCACAAA GCATCCACCG GTGGCTGTCC TGCCTCTTGG GACAGGGAAC 
GACCTCGCAC GATGTCTTCG CTGGGGAGGA GGTTATGAGG GGGGCAGCTT GACCAAAATC 
CTGAAGGAAA TTGAACAGAG CCCCTTGGTG ATGCTGGACC GTTGGTACCT AGAGGTCATG 
CCCAGGGAAG AGGTGGAGAA CGGAGACCAG GTCCCATACA ACATCATGAA CAACTATTTC 
TCCATTGGTG TGGATGCTTC AATTGCACAC AGATTCCATG TGATGAGAGA GAAACACCCT 
GAAAAATTCA ACAGCAGGAT GAAGAACAAG CTGTGGTATT TCGAATTTGG TACCTCTGAA 
ACCTTTGCAG CTACCTGCAA GAAACTCCAT GACCACATAG AGCTGGAGTG CGATGGTGTT 
GAGGTGGACC TGAGCAATAT CTTCCTGGAA GGCATTGCCA TTCTTAACAT TCCCAGCATG 
TACGGAGGCA CCAATCTCTG GGGAGAAACC AAGAAGAACC GAGCTGTGAT CCGGGAAAGC 
AGGAAGAGCG TCACAGACCC AAAGGAGCTG AAATGTTGTG TCCAAGACCT CAGTGACCAG 
CTTCTTGAAG TGGTGGGTCT AGAGGGAGCC ATGGAGATGG GACAGATCTA TACCGGCCTG 
AAGAGTGCAG GCAGGAGGCT AGCCCAGTGC TCCTCTGTCA CCATTAGGAC TAAGAAGCTT 
CTGCCGATGC AAGTGGATGG AGAACCCTGG ATGCAGCCGC CATGCATGAT TAAAATCACT 
CACAAAAACC AGGCACCTAT GATGATGGGG CCTCCCCAGA AGAGCAGCTT CTTTTCTCTG 
AGGAGGAAAA GCCGTTCAAA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037258.1
CDS	308..2674
Translation	MSDGQWVCLSSEEFDQLQKYSEYSSKKIKDVLAEFNEGGSLKQYDPHKPISYDVFKLFMRAYLEVDLPQPLSTNLFLAFSQKPRQETPDHPKEGASSSEPNVSDSNAESTAKADAACAPDTESKPIKTQVPSEELEAAAPWGEPNAPASSSDAPIVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENELLDQAELDQIVSQMLHVAQYLEWDPTELRPILKEMLQGMDYNKDGFVSLEEWVSGGMTTIPLLVLLGMDDSASKGDGRHAWTLKHFKKPTYCNFCHIMLMGVRKQGLCCIYCKYAVHQRCVSNSIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELSTACDGGELKDHILLPTSIYPVTRDRQAGKSDSGAAAKGELVMQYKIIPSPGTHPLLVLVNPKSGGRQGERILQKFHYLLNPKQVFNLDKGGPTPGLNFFQDTPDFRVLACGGDGTVGWILDCIDKANFTKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEVMPREEVENGDQVPYNIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLWGETKKNRAVIRESRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTKKLLPMQVDGEPWMQPPCMIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD

Target ORF information:

RefSeq Version	NM_013126.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013126.1

ORF Insert Sequence:

ATGAGTGACG GGCAATGGGT CTGTCTCTCA TCAGAAGAAT TTGATCAACT TCAGAAATAC 
TCTGAATATT CCTCCAAGAA GATAAAAGAT GTGTTGGCTG AGTTTAATGA AGGTGGAAGC 
CTCAAGCAAT ATGACCCACA CAAGCCCATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCATACCTGG AGGTGGATCT TCCTCAGCCA CTGAGCACAA ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACAAGAGAC ACCTGACCAC CCAAAGGAGG GGGCCAGCAG TAGTGAGCCG 
AATGTCTCAG ATTCTAATGC GGAGAGCACT GCCAAAGCGG ATGCTGCCTG CGCTCCAGAC 
ACTGAATCAA AACCGATCAA GACACAGGTA CCGTCTGAAG AACTAGAGGC TGCAGCCCCA 
TGGGGAGAAC CTAACGCTCC GGCTTCAAGC TCCGATGCCC CCATAGTGTA CCTGAAGGAT 
GTGGTGTGTT ACTTGTCCCT GATGGAGACC GGGAGGCCTC AGGATAAACT AGAATTCATG 
TTTCGTCTCT ATGACTCGGA TGAAAATGAA CTCCTGGACC AAGCGGAGCT GGATCAAATT 
GTGAGCCAAA TGCTACATGT TGCCCAGTAC CTGGAGTGGG ATCCCACAGA GCTCAGACCT 
ATACTGAAGG AGATGTTGCA AGGCATGGAC TACAACAAGG ATGGCTTCGT GTCTCTAGAG 
GAGTGGGTCA GTGGGGGGAT GACCACCATC CCGTTGCTGG TCCTCCTGGG AATGGATGAC 
TCCGCCTCCA AGGGGGATGG GAGGCACGCC TGGACCCTGA AGCACTTCAA GAAGCCCACT 
TACTGCAACT TCTGCCACAT CATGCTGATG GGCGTTCGGA AGCAAGGCCT GTGCTGCATC 
TACTGTAAAT ATGCTGTCCA CCAACGCTGT GTATCCAACA GCATTCCTGG ATGTGTGAAA 
ACATACTCCA AAGCCAAAAG GAGCGGTGAG GTGATGCAGC ACGCGTGGGT GGAAGGGAAC 
TCCTCCGTCA AGTGTGACCG GTGCCACAAA AGTATCAAGT GCTACCAGAG TGTCACTGCG 
CGGCACTGCG TGTGGTGCCG GATGACGTTT CACCGCAAAT GTGAATTGTC GACAGCGTGC 
GATGGTGGGG AACTCAAAGA CCACATTTTG CTGCCTACCT CCATATACCC TGTCACCCGA 
GACCGGCAAG CAGGGAAGTC CGATAGCGGT GCGGCGGCCA AGGGTGAACT TGTAATGCAG 
TATAAGATCA TCCCAAGTCC GGGGACGCAT CCCCTGCTGG TCCTGGTGAA CCCGAAGAGT 
GGAGGAAGAC AAGGAGAAAG AATTCTTCAG AAATTCCACT ACCTGCTCAA CCCCAAACAA 
GTTTTCAACC TGGACAAAGG GGGGCCTACG CCAGGATTGA ACTTCTTTCA GGATACCCCG 
GACTTCCGTG TTCTTGCCTG TGGCGGAGAT GGGACAGTTG GCTGGATTTT GGATTGCATT 
GACAAGGCCA ACTTCACAAA GCATCCACCG GTGGCTGTCC TGCCTCTTGG GACAGGGAAC 
GACCTCGCAC GATGTCTTCG CTGGGGAGGA GGTTATGAGG GGGGCAGCTT GACCAAAATC 
CTGAAGGAAA TTGAACAGAG CCCCTTGGTG ATGCTGGACC GTTGGTACCT AGAGGTCATG 
CCCAGGGAAG AGGTGGAGAA CGGAGACCAG GTCCCATACA ACATCATGAA CAACTATTTC 
TCCATTGGTG TGGATGCTTC AATTGCACAC AGATTCCATG TGATGAGAGA GAAACACCCT 
GAAAAATTCA ACAGCAGGAT GAAGAACAAG CTGTGGTATT TCGAATTTGG TACCTCTGAA 
ACCTTTGCAG CTACCTGCAA GAAACTCCAT GACCACATAG AGCTGGAGTG CGATGGTGTT 
GAGGTGGACC TGAGCAATAT CTTCCTGGAA GGCATTGCCA TTCTTAACAT TCCCAGCATG 
TACGGAGGCA CCAATCTCTG GGGAGAAACC AAGAAGAACC GAGCTGTGAT CCGGGAAAGC 
AGGAAGAGCG TCACAGACCC AAAGGAGCTG AAATGTTGTG TCCAAGACCT CAGTGACCAG 
CTTCTTGAAG TGGTGGGTCT AGAGGGAGCC ATGGAGATGG GACAGATCTA TACCGGCCTG 
AAGAGTGCAG GCAGGAGGCT AGCCCAGTGC TCCTCTGTCA CCATTAGGAC TAAGAAGCTT 
CTGCCGATGC AAGTGGATGG AGAACCCTGG ATGCAGCCGC CATGCATGAT TAAAATCACT 
CACAAAAACC AGGCACCTAT GATGATGGGG CCTCCCCAGA AGAGCAGCTT CTTTTCTCTG 
AGGAGGAAAA GCCGTTCAAA GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkg ( XM_006248538.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248600.1 Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34398
Clone ID Related Accession (Same CDS sequence)	XM_006248538.3
Accession Version	XM_006248538.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG GGCAATGGGT CTGTCTCTCA TCAGAAGAAT TTGATCAACT TCAGAAATAC 
TCTGAATATT CCTCCAAGAA GATAAAAGAT GTGTTGGCTG AGTTTAATGA AGGTGGAAGC 
CTCAAGCAAT ATGACCCACA CAAGCCCATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCATACCTGG AGGTGGATCT TCCTCAGCCA CTGAGCACAA ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACAAGAGAC ACCTGACCAC CCAAAGGAGG GGGCCAGCAG TAGTGAGCCC 
AATGTCTCAG ATTCTAATGC GGAGAGCACT GCCAAAGCGG ATGCTGCCTG CGCTCCAGAC 
ACTGAATCAA AACCGATCAA GACACAAGTA CCGTCTGAAG AACTAGAGGC TGCAGCCCCA 
TGGGGAGAAC CTAACGCTCC GGCTTCAAGC TCCGATGCCC CCATAGTGTA CCTGAAGGAT 
GTGGTGTGTT ACTTGTCCCT GATGGAGACC GGGAGGCCTC AGGATAAACT AGAATTCATG 
TTTCGTCTCT ATGATTCGGA TGAAAATGAA CTCCTGGACC AAGCGGAGCT GGATCAAATT 
GTGAGCCAAA TGCTACATGT TGCCCAGTAC CTGGAGTGGG ATCCCACAGA GCTCAGACCT 
ATACTGAAGG AGATGTTGCA AGGCATGGAC TACAACAAGG ATGGCTTCGT GTCTCTAGAG 
GAGTGGGTCA GTGGGGGGAT GACCACCATC CCGTTGCTGG TCCTCCTGGG AATGGATGAC 
TCCGCCTCCA AGGGGGATGG GAGGCACGCC TGGACCCTGA AGCACTTCAA GAAGCCCACT 
TACTGCAACT TCTGCCACAT CATGCTGATG GGCGTTCGGA AGCAAGGCCT GTGCTGCATC 
TACTGTAAAT ATGCTGTCCA CCAACGCTGT GTATCCAACA GCATTCCTGG ATGTGTGAAA 
ACATACTCCA AAGCCAAAAG GAGCGGTGAG TTTCACCGCA AATGTGAATT GTCGACAGCG 
TGCGATGGTG GGGAACTCAA AGACCACATT TTGCTGCCTA CCTCCATATA CCCTGTCACC 
CGAGACCGGC AAGCAGGGAA GTCCGATAGC GGTGCGGCCG CCAAGGGTGA ACTTGTAATG 
CAGTATAAGA TCATCCCAAG TCCGGGGACG CATCCCCTGC TGGTCCTGGT GAACCCCAAG 
AGTGGAGGAA GACAAGGAGA AAGAATTCTT CAGAAATTCC ACTACCTGCT CAACCCCAAA 
CAAGTTTTCA ACCTGGACAA AGGGGGGCCT ACGCCAGGAT TGAACTTCTT TCAGGATACC 
CCGGACTTCC GTGTTCTTGC CTGTGGCGGA GATGGGACAG TTGGCTGGAT TTTGGATTGC 
ATTGACAAGG CCAACTTCAC AAAGCATCCA CCGGTGGCTG TCCTGCCTCT TGGGACAGGG 
AACGACCTCG CACGATGTCT TCGCTGGGGA GGAGGTTATG AGGGGGGCAG CTTGACCAAA 
ATCCTGAAGG AAATTGAACA GAGCCCCTTG GTGATGCTGG ACCGTTGGTA CCTAGAGGTC 
ATGCCCAGGG AAGAGGTGGA GAACGGAGAC CAGGTCCCAT ACAACATCAT GAACAACTAT 
TTCTCCATTG GTGTGGATGC TTCAATTGCA CACAGATTCC ATGTGATGAG AGAGAAACAC 
CCTGAAAAAT TCAACAGCAG GATGAAGAAC AAGCTGTGGT ATTTCGAATT TGGTACCTCT 
GAAACCTTTG CAGCTACCTG CAAGAAACTC CATGACCACA TAGAGCTGGA GTGCGATGGC 
GTTGAGGTGG ACCTGAGCAA TATCTTCCTG GAAGGCATTG CCATTCTTAA CATTCCCAGC 
ATGTACGGAG GCACCAATCT CTGGGGAGAA ACCAAGAAGA ACCGAGCTGT GATCCGGGAA 
AGCAGGAAGA GCGTCACAGA CCCAAAGGAG CTGAAATGTT GTGTCCAAGA CCTCAGTGAC 
CAGCTTCTTG AAGTGGTGGG TCTAGAGGGA GCCATGGAGA TGGGACAGAT CTATACCGGC 
CTGAAGAGTG CAGGCAGGAG GCTAGCCCAG TGCTCCTCTG TCACCATTAG GACTAAGAAG 
CTTCTGCCGA TGCAAGTGGA TGGAGAACCC TGGATGCAGC CGCCATGCAT GATTAAAATC 
ACTCACAAAA ACCAGGCACC TATGATGATG GGGCCTCCCC AGAAGAGCAG CTTCTTTTCT 
CTGAGGAGGA AAAGCCGTTC AAAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248600.1
CDS	604..2853
Translation	MSDGQWVCLSSEEFDQLQKYSEYSSKKIKDVLAEFNEGGSLKQYDPHKPISYDVFKLFMRAYLEVDLPQPLSTNLFLAFSQKPRQETPDHPKEGASSSEPNVSDSNAESTAKADAACAPDTESKPIKTQVPSEELEAAAPWGEPNAPASSSDAPIVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENELLDQAELDQIVSQMLHVAQYLEWDPTELRPILKEMLQGMDYNKDGFVSLEEWVSGGMTTIPLLVLLGMDDSASKGDGRHAWTLKHFKKPTYCNFCHIMLMGVRKQGLCCIYCKYAVHQRCVSNSIPGCVKTYSKAKRSGEFHRKCELSTACDGGELKDHILLPTSIYPVTRDRQAGKSDSGAAAKGELVMQYKIIPSPGTHPLLVLVNPKSGGRQGERILQKFHYLLNPKQVFNLDKGGPTPGLNFFQDTPDFRVLACGGDGTVGWILDCIDKANFTKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEVMPREEVENGDQVPYNIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLWGETKKNRAVIRESRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTKKLLPMQVDGEPWMQPPCMIKITHKNQAPMMMGPPQKSSFFSLRRKSRSKD

Target ORF information:

RefSeq Version	XM_006248538.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248538.3

ORF Insert Sequence:

ATGAGTGACG GGCAATGGGT CTGTCTCTCA TCAGAAGAAT TTGATCAACT TCAGAAATAC 
TCTGAATATT CCTCCAAGAA GATAAAAGAT GTGTTGGCTG AGTTTAATGA AGGTGGAAGC 
CTCAAGCAAT ATGACCCACA CAAGCCCATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCATACCTGG AGGTGGATCT TCCTCAGCCA CTGAGCACAA ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACAAGAGAC ACCTGACCAC CCAAAGGAGG GGGCCAGCAG TAGTGAGCCC 
AATGTCTCAG ATTCTAATGC GGAGAGCACT GCCAAAGCGG ATGCTGCCTG CGCTCCAGAC 
ACTGAATCAA AACCGATCAA GACACAAGTA CCGTCTGAAG AACTAGAGGC TGCAGCCCCA 
TGGGGAGAAC CTAACGCTCC GGCTTCAAGC TCCGATGCCC CCATAGTGTA CCTGAAGGAT 
GTGGTGTGTT ACTTGTCCCT GATGGAGACC GGGAGGCCTC AGGATAAACT AGAATTCATG 
TTTCGTCTCT ATGATTCGGA TGAAAATGAA CTCCTGGACC AAGCGGAGCT GGATCAAATT 
GTGAGCCAAA TGCTACATGT TGCCCAGTAC CTGGAGTGGG ATCCCACAGA GCTCAGACCT 
ATACTGAAGG AGATGTTGCA AGGCATGGAC TACAACAAGG ATGGCTTCGT GTCTCTAGAG 
GAGTGGGTCA GTGGGGGGAT GACCACCATC CCGTTGCTGG TCCTCCTGGG AATGGATGAC 
TCCGCCTCCA AGGGGGATGG GAGGCACGCC TGGACCCTGA AGCACTTCAA GAAGCCCACT 
TACTGCAACT TCTGCCACAT CATGCTGATG GGCGTTCGGA AGCAAGGCCT GTGCTGCATC 
TACTGTAAAT ATGCTGTCCA CCAACGCTGT GTATCCAACA GCATTCCTGG ATGTGTGAAA 
ACATACTCCA AAGCCAAAAG GAGCGGTGAG TTTCACCGCA AATGTGAATT GTCGACAGCG 
TGCGATGGTG GGGAACTCAA AGACCACATT TTGCTGCCTA CCTCCATATA CCCTGTCACC 
CGAGACCGGC AAGCAGGGAA GTCCGATAGC GGTGCGGCCG CCAAGGGTGA ACTTGTAATG 
CAGTATAAGA TCATCCCAAG TCCGGGGACG CATCCCCTGC TGGTCCTGGT GAACCCCAAG 
AGTGGAGGAA GACAAGGAGA AAGAATTCTT CAGAAATTCC ACTACCTGCT CAACCCCAAA 
CAAGTTTTCA ACCTGGACAA AGGGGGGCCT ACGCCAGGAT TGAACTTCTT TCAGGATACC 
CCGGACTTCC GTGTTCTTGC CTGTGGCGGA GATGGGACAG TTGGCTGGAT TTTGGATTGC 
ATTGACAAGG CCAACTTCAC AAAGCATCCA CCGGTGGCTG TCCTGCCTCT TGGGACAGGG 
AACGACCTCG CACGATGTCT TCGCTGGGGA GGAGGTTATG AGGGGGGCAG CTTGACCAAA 
ATCCTGAAGG AAATTGAACA GAGCCCCTTG GTGATGCTGG ACCGTTGGTA CCTAGAGGTC 
ATGCCCAGGG AAGAGGTGGA GAACGGAGAC CAGGTCCCAT ACAACATCAT GAACAACTAT 
TTCTCCATTG GTGTGGATGC TTCAATTGCA CACAGATTCC ATGTGATGAG AGAGAAACAC 
CCTGAAAAAT TCAACAGCAG GATGAAGAAC AAGCTGTGGT ATTTCGAATT TGGTACCTCT 
GAAACCTTTG CAGCTACCTG CAAGAAACTC CATGACCACA TAGAGCTGGA GTGCGATGGC 
GTTGAGGTGG ACCTGAGCAA TATCTTCCTG GAAGGCATTG CCATTCTTAA CATTCCCAGC 
ATGTACGGAG GCACCAATCT CTGGGGAGAA ACCAAGAAGA ACCGAGCTGT GATCCGGGAA 
AGCAGGAAGA GCGTCACAGA CCCAAAGGAG CTGAAATGTT GTGTCCAAGA CCTCAGTGAC 
CAGCTTCTTG AAGTGGTGGG TCTAGAGGGA GCCATGGAGA TGGGACAGAT CTATACCGGC 
CTGAAGAGTG CAGGCAGGAG GCTAGCCCAG TGCTCCTCTG TCACCATTAG GACTAAGAAG 
CTTCTGCCGA TGCAAGTGGA TGGAGAACCC TGGATGCAGC CGCCATGCAT GATTAAAATC 
ACTCACAAAA ACCAGGCACC TATGATGATG GGGCCTCCCC AGAAGAGCAG CTTCTTTTCT 
CTGAGGAGGA AAAGCCGTTC AAAGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkg ( XM_006248539.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248601.1 Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa34399
Clone ID Related Accession (Same CDS sequence)	XM_006248539.3
Accession Version	XM_006248539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase gamma isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248539.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG GGCAATGGGT CTGTCTCTCA TCAGAAGAAT TTGATCAACT TCAGAAATAC 
TCTGAATATT CCTCCAAGAA GATAAAAGAT GTGTTGGCTG AGTTTAATGA AGGTGGAAGC 
CTCAAGCAAT ATGACCCACA CAAGCCCATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCATACCTGG AGGTGGATCT TCCTCAGCCA CTGAGCACAA ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACAAGAGAC ACCTGACCAC CCAAAGGAGG GGGCCAGCAG TAGTGAGCCC 
AATGTCTCAG ATTCTAATGC GGAGAGCACT GCCAAAGCGG ATGCTGCCTG CGCTCCAGAC 
ACTGAATCAA AACCGATCAA GACACAAGTA CCGTCTGAAG AACTAGAGGC TGCAGCCCCA 
TGGGGAGAAC CTAACGCTCC GGCTTCAAGC TCCGATGCCC CCATAGTGTA CCTGAAGGAT 
GTGGTGTGTT ACTTGTCCCT GATGGAGACC GGGAGGCCTC AGGATAAACT AGAATTCATG 
TTTCGTCTCT ATGATTCGGA TGAAAATGAA CTCCTGGACC AAGCGGAGCT GGATCAAATT 
GTGAGCCAAA TGCTACATGT TGCCCAGTAC CTGGAGTGGG ATCCCACAGA GCTCAGACCT 
ATACTGAAGG AGATGTTGCA AGGCATGGAC TACAACAAGG ATGGCTTCGT GTCTCTAGAG 
GAGTGGGTCA GTGGGGGGAT GACCACCATC CCGTTGCTGG TCCTCCTGGG AATGGATGAC 
TCCGCCTCCA AGGGGGATGG GAGGCACGCC TGGACCCTGA AGCACTTCAA GAAGCCCACT 
TACTGCAACT TCTGCCACAT CATGCTGATG GGCGTTCGGA AGCAAGGCCT GTGCTGCATC 
TACTGTAAAT ATGCTGTCCA CCAACGCTGT GTATCCAACA GCATTCCTGG ATGTGTGAAA 
ACATACTCCA AAGCCAAAAG GAGCGGTGAG GTGATGCAGC ACGCGTGGGT GGAAGGGAAC 
TCCTCCGTCA AGTGTGACCG GTGCCACAAA AGTATCAAGT GCTACCAGAG TGTCACCGCG 
CGGCACTGCG TGTGGTGCCG GATGACGTTT CACCGCAAAT GTGAATTGTC GACAGCGTGC 
GATGGTGGGG AACTCAAAGA CCACATTTTG CTGCCTACCT CCATATACCC TGTCACCCGA 
GACCGGCAAG CAGGGAAGTC CGATAGCGGT GCGGCCGCCA AGGGTGAACT TGTAATGCAG 
TATAAGATCA TCCCAAGTCC GGGGACGCAT CCCCTGCTGG TCCTGGTGAA CCCCAAGAGT 
GGAGGAAGAC AAGGAGAAAG AATTCTTCAG AAATTCCACT ACCTGCTCAA CCCCAAACAA 
GTTTTCAACC TGGACAAAGG GGGGCCTACG CCAGGATTGA ACTTCTTTCA GGATACCCCG 
GACTTCCGTG TTCTTGCCTG TGGCGGAGAT GGGACAGTTG GCTGGATTTT GGATTGCATT 
GACAAGGCCA ACTTCACAAA GCATCCACCG GTGGCTGTCC TGCCTCTTGG GACAGGGAAC 
GACCTCGCAC GATGTCTTCG CTGGGGAGGA GGTTATGAGG GGGGCAGCTT GACCAAAATC 
CTGAAGGAAA TTGAACAGAG CCCCTTGGTG ATGCTGGACC GTTGGTACCT AGAGGTCATG 
CCCAGGGAAG AGGTGGAGAA CGGAGACCAG GTCCCATACA ACATCATGAA CAACTATTTC 
TCCATTGGTG TGGATGCTTC AATTGCACAC AGATTCCATG TGATGAGAGA GAAACACCCT 
GAAAAATTCA ACAGCAGGAT GAAGAACAAG CTGTGGTATT TCGAATTTGG TACCTCTGAA 
ACCTTTGCAG CTACCTGCAA GAAACTCCAT GACCACATAG AGCTGGAGTG CGATGGCGTT 
GAGGTGGACC TGAGCAATAT CTTCCTGGAA GGCATTGCCA TTCTTAACAT TCCCAGCATG 
TACGGAGGCA CCAATCTCTG GGGAGAAACC AAGAAGAACC GAGCTGTGAT CCGGGAAAGC 
AGGAAGAGCG TCACAGACCC AAAGGAGCTG AAATGTTGTG TCCAAGCTTC CTGTAGTGTT 
CCTGCCTTGG TCCATTTTCT TCAGCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248601.1
CDS	604..2733
Translation	MSDGQWVCLSSEEFDQLQKYSEYSSKKIKDVLAEFNEGGSLKQYDPHKPISYDVFKLFMRAYLEVDLPQPLSTNLFLAFSQKPRQETPDHPKEGASSSEPNVSDSNAESTAKADAACAPDTESKPIKTQVPSEELEAAAPWGEPNAPASSSDAPIVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENELLDQAELDQIVSQMLHVAQYLEWDPTELRPILKEMLQGMDYNKDGFVSLEEWVSGGMTTIPLLVLLGMDDSASKGDGRHAWTLKHFKKPTYCNFCHIMLMGVRKQGLCCIYCKYAVHQRCVSNSIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELSTACDGGELKDHILLPTSIYPVTRDRQAGKSDSGAAAKGELVMQYKIIPSPGTHPLLVLVNPKSGGRQGERILQKFHYLLNPKQVFNLDKGGPTPGLNFFQDTPDFRVLACGGDGTVGWILDCIDKANFTKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEVMPREEVENGDQVPYNIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLWGETKKNRAVIRESRKSVTDPKELKCCVQASCSVPALVHFLQL

Target ORF information:

RefSeq Version	XM_006248539.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248539.3

ORF Insert Sequence:

ATGAGTGACG GGCAATGGGT CTGTCTCTCA TCAGAAGAAT TTGATCAACT TCAGAAATAC 
TCTGAATATT CCTCCAAGAA GATAAAAGAT GTGTTGGCTG AGTTTAATGA AGGTGGAAGC 
CTCAAGCAAT ATGACCCACA CAAGCCCATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCATACCTGG AGGTGGATCT TCCTCAGCCA CTGAGCACAA ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACAAGAGAC ACCTGACCAC CCAAAGGAGG GGGCCAGCAG TAGTGAGCCC 
AATGTCTCAG ATTCTAATGC GGAGAGCACT GCCAAAGCGG ATGCTGCCTG CGCTCCAGAC 
ACTGAATCAA AACCGATCAA GACACAAGTA CCGTCTGAAG AACTAGAGGC TGCAGCCCCA 
TGGGGAGAAC CTAACGCTCC GGCTTCAAGC TCCGATGCCC CCATAGTGTA CCTGAAGGAT 
GTGGTGTGTT ACTTGTCCCT GATGGAGACC GGGAGGCCTC AGGATAAACT AGAATTCATG 
TTTCGTCTCT ATGATTCGGA TGAAAATGAA CTCCTGGACC AAGCGGAGCT GGATCAAATT 
GTGAGCCAAA TGCTACATGT TGCCCAGTAC CTGGAGTGGG ATCCCACAGA GCTCAGACCT 
ATACTGAAGG AGATGTTGCA AGGCATGGAC TACAACAAGG ATGGCTTCGT GTCTCTAGAG 
GAGTGGGTCA GTGGGGGGAT GACCACCATC CCGTTGCTGG TCCTCCTGGG AATGGATGAC 
TCCGCCTCCA AGGGGGATGG GAGGCACGCC TGGACCCTGA AGCACTTCAA GAAGCCCACT 
TACTGCAACT TCTGCCACAT CATGCTGATG GGCGTTCGGA AGCAAGGCCT GTGCTGCATC 
TACTGTAAAT ATGCTGTCCA CCAACGCTGT GTATCCAACA GCATTCCTGG ATGTGTGAAA 
ACATACTCCA AAGCCAAAAG GAGCGGTGAG GTGATGCAGC ACGCGTGGGT GGAAGGGAAC 
TCCTCCGTCA AGTGTGACCG GTGCCACAAA AGTATCAAGT GCTACCAGAG TGTCACCGCG 
CGGCACTGCG TGTGGTGCCG GATGACGTTT CACCGCAAAT GTGAATTGTC GACAGCGTGC 
GATGGTGGGG AACTCAAAGA CCACATTTTG CTGCCTACCT CCATATACCC TGTCACCCGA 
GACCGGCAAG CAGGGAAGTC CGATAGCGGT GCGGCCGCCA AGGGTGAACT TGTAATGCAG 
TATAAGATCA TCCCAAGTCC GGGGACGCAT CCCCTGCTGG TCCTGGTGAA CCCCAAGAGT 
GGAGGAAGAC AAGGAGAAAG AATTCTTCAG AAATTCCACT ACCTGCTCAA CCCCAAACAA 
GTTTTCAACC TGGACAAAGG GGGGCCTACG CCAGGATTGA ACTTCTTTCA GGATACCCCG 
GACTTCCGTG TTCTTGCCTG TGGCGGAGAT GGGACAGTTG GCTGGATTTT GGATTGCATT 
GACAAGGCCA ACTTCACAAA GCATCCACCG GTGGCTGTCC TGCCTCTTGG GACAGGGAAC 
GACCTCGCAC GATGTCTTCG CTGGGGAGGA GGTTATGAGG GGGGCAGCTT GACCAAAATC 
CTGAAGGAAA TTGAACAGAG CCCCTTGGTG ATGCTGGACC GTTGGTACCT AGAGGTCATG 
CCCAGGGAAG AGGTGGAGAA CGGAGACCAG GTCCCATACA ACATCATGAA CAACTATTTC 
TCCATTGGTG TGGATGCTTC AATTGCACAC AGATTCCATG TGATGAGAGA GAAACACCCT 
GAAAAATTCA ACAGCAGGAT GAAGAACAAG CTGTGGTATT TCGAATTTGG TACCTCTGAA 
ACCTTTGCAG CTACCTGCAA GAAACTCCAT GACCACATAG AGCTGGAGTG CGATGGCGTT 
GAGGTGGACC TGAGCAATAT CTTCCTGGAA GGCATTGCCA TTCTTAACAT TCCCAGCATG 
TACGGAGGCA CCAATCTCTG GGGAGAAACC AAGAAGAACC GAGCTGTGAT CCGGGAAAGC 
AGGAAGAGCG TCACAGACCC AAAGGAGCTG AAATGTTGTG TCCAAGCTTC CTGTAGTGTT 
CCTGCCTTGG TCCATTTTCT TCAGCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkg ( XM_006248540.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248602.1 Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa34400
Clone ID Related Accession (Same CDS sequence)	XM_006248540.3
Accession Version	XM_006248540.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase gamma isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248540.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACG GGCAATGGGT CTGTCTCTCA TCAGAAGAAT TTGATCAACT TCAGAAATAC 
TCTGAATATT CCTCCAAGAA GATAAAAGAT GTGTTGGCTG AGTTTAATGA AGGTGGAAGC 
CTCAAGCAAT ATGACCCACA CAAGCCCATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCATACCTGG AGGTGGATCT TCCTCAGCCA CTGAGCACAA ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACAAGAGAC ACCTGACCAC CCAAAGGAGG GGGCCAGCAG TAGTGAGCCC 
AATGTCTCAG ATTCTAATGC GGAGAGCACT GCCAAAGCGG ATGCTGCCTG CGCTCCAGAC 
ACTGAATCAA AACCGATCAA GACACAAGTA CCGTCTGAAG AACTAGAGGC TGCAGCCCCA 
TGGGGAGAAC CTAACGCTCC GGCTTCAAGC TCCGATGCCC CCATAGTGTA CCTGAAGGAT 
GTGGTGTGTT ACTTGTCCCT GATGGAGACC GGGAGGCCTC AGGATAAACT AGAATTCATG 
TTTCGTCTCT ATGATTCGGA TGAAAATGAA CTCCTGGACC AAGCGGAGCT GGATCAAATT 
GTGAGCCAAA TGCTACATGT TGCCCAGTAC CTGGAGTGGG ATCCCACAGA GCTCAGACCT 
ATACTGAAGG AGATGTTGCA AGGCATGGAC TACAACAAGG ATGGCTTCGT GTCTCTAGAG 
GAGTGGGTCA GTGGGGGGAT GACCACCATC CCGTTGCTGG TCCTCCTGGG AATGGATGAC 
TCCGCCTCCA AGGGGGATGG GAGGCACGCC TGGACCCTGA AGCACTTCAA GAAGCCCACT 
TACTGCAACT TCTGCCACAT CATGCTGATG GGCGTTCGGA AGCAAGGCCT GTGCTGCATC 
TACTGTAAAT ATGCTGTCCA CCAACGCTGT GTATCCAACA GCATTCCTGG ATGTGTGAAA 
ACATACTCCA AAGCCAAAAG GAGCGGTGAG GTGATGCAGC ACGCGTGGGT GGAAGGGAAC 
TCCTCCGTCA AGTGTGACCG GTGCCACAAA AGTATCAAGT GCTACCAGAG TGTCACCGCG 
CGGCACTGCG TGTGGTGCCG GATGACGTTT CACCGCAAAT GTGAATTGTC GACAGCGTGC 
GATGGTGGGG AACTCAAAGA CCACATTTTG CTGCCTACCT CCATATACCC TGTCACCCGA 
GACCGGCAAG CAGGGAAGTC CGATAGCGGT GCGGCCGCCA AGGGTGAACT TGTAATGCAG 
TATAAGATCA TCCCAAGTCC GGGGACGCAT CCCCTGCTGG TCCTGGTGAA CCCCAAGAGT 
GGAGGAAGAC AAGGAGAAAG AATTCTTCAG AAATTCCACT ACCTGCTCAA CCCCAAACAA 
GTTTTCAACC TGGACAAAGG GGGGCCTACG CCAGGATTGA ACTTCTTTCA GGATACCCCG 
GACTTCCGTG TTCTTGCCTG TGGCGGAGAT GGGACAGTTG GCTGGATTTT GGATTGCATT 
GACAAGGCCA ACTTCACAAA GCATCCACCG GTGGCTGTCC TGCCTCTTGG GACAGGGAAC 
GACCTCGCAC GATGTCTTCG CTGGGGAGGA GGTTATGAGG GGGGCAGCTT GACCAAAATC 
CTGAAGGAAA TTGAACAGAG CCCCTTGGTG ATGCTGGACC GTTGGTACCT AGAGGTCATG 
CCCAGGGAAG AGGTGGAGAA CGGAGACCAG GTCCCATACA ACATCATGAA CAACTATTTC 
TCCATTGGTG TGGATGCTTC AATTGCACAC AGATTCCATG TGATGAGAGA GAAACACCCT 
GAAAAATTCA ACAGCAGGAT TCCGTGTGTC CACCTGCTCC CATCCTCCTC CCCATCTGGG 
GACTTGGCCT GGCATGTTGT GGTGTTACTA ATCTGTTTCA GAACCCCTGG CACTGTCCAG 
CTTGGTCCTC CTGGTGCCCA GACAGTCCTT CCCCATAGAA ACGATGCTCA TAGTGAACAG 
GCCCCTCTTG ATGATCCACG TGATGTGTGG GCGAGTGCCA TGGCCTCTTG GGAAGTGGTC 
CTGACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248602.1
CDS	944..2992
Translation	MSDGQWVCLSSEEFDQLQKYSEYSSKKIKDVLAEFNEGGSLKQYDPHKPISYDVFKLFMRAYLEVDLPQPLSTNLFLAFSQKPRQETPDHPKEGASSSEPNVSDSNAESTAKADAACAPDTESKPIKTQVPSEELEAAAPWGEPNAPASSSDAPIVYLKDVVCYLSLMETGRPQDKLEFMFRLYDSDENELLDQAELDQIVSQMLHVAQYLEWDPTELRPILKEMLQGMDYNKDGFVSLEEWVSGGMTTIPLLVLLGMDDSASKGDGRHAWTLKHFKKPTYCNFCHIMLMGVRKQGLCCIYCKYAVHQRCVSNSIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQSVTARHCVWCRMTFHRKCELSTACDGGELKDHILLPTSIYPVTRDRQAGKSDSGAAAKGELVMQYKIIPSPGTHPLLVLVNPKSGGRQGERILQKFHYLLNPKQVFNLDKGGPTPGLNFFQDTPDFRVLACGGDGTVGWILDCIDKANFTKHPPVAVLPLGTGNDLARCLRWGGGYEGGSLTKILKEIEQSPLVMLDRWYLEVMPREEVENGDQVPYNIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRIPCVHLLPSSSPSGDLAWHVVVLLICFRTPGTVQLGPPGAQTVLPHRNDAHSEQAPLDDPRDVWASAMASWEVVLT

Target ORF information:

RefSeq Version	XM_006248540.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, gamma (Dgkg), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248540.3

ORF Insert Sequence:

ATGAGTGACG GGCAATGGGT CTGTCTCTCA TCAGAAGAAT TTGATCAACT TCAGAAATAC 
TCTGAATATT CCTCCAAGAA GATAAAAGAT GTGTTGGCTG AGTTTAATGA AGGTGGAAGC 
CTCAAGCAAT ATGACCCACA CAAGCCCATT AGCTATGATG TCTTCAAGCT GTTCATGAGG 
GCATACCTGG AGGTGGATCT TCCTCAGCCA CTGAGCACAA ACCTCTTCCT GGCCTTCAGC 
CAGAAGCCCA GACAAGAGAC ACCTGACCAC CCAAAGGAGG GGGCCAGCAG TAGTGAGCCC 
AATGTCTCAG ATTCTAATGC GGAGAGCACT GCCAAAGCGG ATGCTGCCTG CGCTCCAGAC 
ACTGAATCAA AACCGATCAA GACACAAGTA CCGTCTGAAG AACTAGAGGC TGCAGCCCCA 
TGGGGAGAAC CTAACGCTCC GGCTTCAAGC TCCGATGCCC CCATAGTGTA CCTGAAGGAT 
GTGGTGTGTT ACTTGTCCCT GATGGAGACC GGGAGGCCTC AGGATAAACT AGAATTCATG 
TTTCGTCTCT ATGATTCGGA TGAAAATGAA CTCCTGGACC AAGCGGAGCT GGATCAAATT 
GTGAGCCAAA TGCTACATGT TGCCCAGTAC CTGGAGTGGG ATCCCACAGA GCTCAGACCT 
ATACTGAAGG AGATGTTGCA AGGCATGGAC TACAACAAGG ATGGCTTCGT GTCTCTAGAG 
GAGTGGGTCA GTGGGGGGAT GACCACCATC CCGTTGCTGG TCCTCCTGGG AATGGATGAC 
TCCGCCTCCA AGGGGGATGG GAGGCACGCC TGGACCCTGA AGCACTTCAA GAAGCCCACT 
TACTGCAACT TCTGCCACAT CATGCTGATG GGCGTTCGGA AGCAAGGCCT GTGCTGCATC 
TACTGTAAAT ATGCTGTCCA CCAACGCTGT GTATCCAACA GCATTCCTGG ATGTGTGAAA 
ACATACTCCA AAGCCAAAAG GAGCGGTGAG GTGATGCAGC ACGCGTGGGT GGAAGGGAAC 
TCCTCCGTCA AGTGTGACCG GTGCCACAAA AGTATCAAGT GCTACCAGAG TGTCACCGCG 
CGGCACTGCG TGTGGTGCCG GATGACGTTT CACCGCAAAT GTGAATTGTC GACAGCGTGC 
GATGGTGGGG AACTCAAAGA CCACATTTTG CTGCCTACCT CCATATACCC TGTCACCCGA 
GACCGGCAAG CAGGGAAGTC CGATAGCGGT GCGGCCGCCA AGGGTGAACT TGTAATGCAG 
TATAAGATCA TCCCAAGTCC GGGGACGCAT CCCCTGCTGG TCCTGGTGAA CCCCAAGAGT 
GGAGGAAGAC AAGGAGAAAG AATTCTTCAG AAATTCCACT ACCTGCTCAA CCCCAAACAA 
GTTTTCAACC TGGACAAAGG GGGGCCTACG CCAGGATTGA ACTTCTTTCA GGATACCCCG 
GACTTCCGTG TTCTTGCCTG TGGCGGAGAT GGGACAGTTG GCTGGATTTT GGATTGCATT 
GACAAGGCCA ACTTCACAAA GCATCCACCG GTGGCTGTCC TGCCTCTTGG GACAGGGAAC 
GACCTCGCAC GATGTCTTCG CTGGGGAGGA GGTTATGAGG GGGGCAGCTT GACCAAAATC 
CTGAAGGAAA TTGAACAGAG CCCCTTGGTG ATGCTGGACC GTTGGTACCT AGAGGTCATG 
CCCAGGGAAG AGGTGGAGAA CGGAGACCAG GTCCCATACA ACATCATGAA CAACTATTTC 
TCCATTGGTG TGGATGCTTC AATTGCACAC AGATTCCATG TGATGAGAGA GAAACACCCT 
GAAAAATTCA ACAGCAGGAT TCCGTGTGTC CACCTGCTCC CATCCTCCTC CCCATCTGGG 
GACTTGGCCT GGCATGTTGT GGTGTTACTA ATCTGTTTCA GAACCCCTGG CACTGTCCAG 
CTTGGTCCTC CTGGTGCCCA GACAGTCCTT CCCCATAGAA ACGATGCTCA TAGTGAACAG 
GCCCCTCTTG ATGATCCACG TGATGTGTGG GCGAGTGCCA TGGCCTCTTG GGAAGTGGTC 
CTGACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diacylglycerol kinase γ regulates antigen-induced mast cell degranulation by mediating Ca(2+) influxes.
Biochemical and biophysical research communications445(2)340-5(2014 Mar)
Sakuma M,Shirai Y,Ueyama T,Saito N

Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.
Biochemical and biophysical research communications422(4)758-63(2012 Jun)
Gellett AM,Kharel Y,Sunkara M,Morris AJ,Lynch KR

Phosphorylation and up-regulation of diacylglycerol kinase gamma via its interaction with protein kinase C gamma.
The Journal of biological chemistry281(42)31627-37(2006 Oct)
Yamaguchi Y,Shirai Y,Matsubara T,Sanse K,Kuriyama M,Oshiro N,Yoshino K,Yonezawa K,Ono Y,Saito N

Immunocytochemical localization of a neuron-specific diacylglycerol kinase beta and gamma in the developing rat brain.
Brain research. Molecular brain research139(2)288-99(2005 Oct)
Adachi N,Oyasu M,Taniguchi T,Yamaguchi Y,Takenaka R,Shirai Y,Saito N

High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence.
Genome research14(4)750-7(2004 Apr)
Kwitek AE,Gullings-Handley J,Yu J,Carlos DC,Orlebeke K,Nie J,Eckert J,Lemke A,Andrae JW,Bromberg S,Pasko D,Chen D,Scheetz TE,Casavant TL,Soares MB,Sheffield VC,Tonellato PJ,Jacob HJ

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Dgkg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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