Agrn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agrn
Entrez Gene ID	25592
Full Name	agrin
Synonyms	AGR
General protein information	Preferred Names agrin Names agrin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	may play a role in synapse development and regeneration [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239541.3	XP_006239603.1	agrin isoform X5
XM_017593166.1	XP_017448655.1	agrin isoform X1
XM_017593167.1	XP_017448656.1	agrin isoform X2
NM_175754.1	NP_786930.1	agrin precursor
XM_017593169.1	XP_017448658.1	agrin isoform X4
XM_017593168.1	XP_017448657.1	agrin isoform X3
XM_006239542.3	XP_006239604.1	agrin isoform X6
XM_017593170.1	XP_017448659.1	agrin isoform X7

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AGRN	XP_005620416.1
Caenorhabditis elegans (roundworm)	agr-1	NP_001022152.3
Homo sapiens (human)	AGRN	NP_940978.2
Rattus norvegicus (Norway rat)	Agrn	NP_786930.1
Xenopus tropicalis (tropical clawed frog)	agrn	XP_002936430.2
Macaca mulatta (Rhesus monkey)	AGRN	XP_001088755.2
Mus musculus (house mouse)	Agrn	NP_067617.3
Gallus gallus (chicken)	AGRN	NP_990858.1
Bos taurus (cattle)	AGRN	XP_005217262.1
Danio rerio (zebrafish)	hm:gc12	NP_001170923.1
Pan troglodytes (chimpanzee)	LOC100609326	XP_003319002.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Agrin requires specific proteins to selectively activate γ-aminobutyric acid neurons for pain suppression.
Erasso D, Tender G, Levitt RC, Cui JG
Experimental neurology261646-53(2014 Nov)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
Soteriou D, Iskender B, Byron A, Humphries JD, Borg-Bartolo S, Haddock MC, Baxter MA, Knight D, Humphries MJ, Kimber SJ
The Journal of biological chemistry288(26)18716-31(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Agrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03921	NM_175754.1 Latest version!	Rattus norvegicus agrin (Agrn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa61112	XM_017593170.1 Latest version!	Rattus norvegicus agrin (Agrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa28987	XM_006239541.3 Latest version!	Rattus norvegicus agrin (Agrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa28988	XM_006239542.3 Latest version!	Rattus norvegicus agrin (Agrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa61108	XM_017593166.1 Latest version!	Rattus norvegicus agrin (Agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa61109	XM_017593167.1 Latest version!	Rattus norvegicus agrin (Agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa61110	XM_017593168.1 Latest version!	Rattus norvegicus agrin (Agrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa61111	XM_017593169.1 Latest version!	Rattus norvegicus agrin (Agrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agrn ( NM_175754.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_786930.1 Rattus norvegicus agrin (Agrn), mRNA.

Price & Availability↑

CloneID	ORa03921
Clone ID Related Accession (Same CDS sequence)	NM_175754.1
Accession Version	NM_175754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Rattus norvegicus(Norway rat)
Product	agrin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M64780.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M64780.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGGAGC CTGGTGCCTC CATGCTGGTT 
CGATACTTCA TGATCCCCTG CAACATCTGC TTGATCCTGT TGGCCACTTC CACATTGGGC 
TTTGCGGTTC TGCTTTTCCT CAGCAACTAC AAACCTGGAA TCCACTTCAC ACCAGCGCCT 
CCTACGCCTC CTGATGTATG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG TGCCTCCTGT GTGTGCAAGA AGAATGCTTG CCCTGCTACG 
GTGGCTCCTG TGTGTGGCTC AGATGCCTCC ACCTATAGCA ACGAGTGTGA GCTGCAGCGC 
GCGCAGTGCA ACCAGCAACG GCGCATCCGC CTGCTTCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAACGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC GGCTGACGGA 
CAGACTGCCT CATGTCTGTG TCCTACAACC TGCTTCGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGACG GTGTTGACTA CCCTAGTGAA TGCCAGCTGC TTAGTCATGC CTGTGCCAGC 
CAGGAGCACA TCTTCAAGAA GTTCAATGGT CCTTGTGATC CCTGCCAGGG CAGCATGTCA 
GACCTGAACC ACATTTGCCG TGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCTGCCCA GCACACGCCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA ACCCGTGGCC TGCTTCTCCA GAAAGTACGC 
TCTGGTCAAT GCCAAACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCCGTATGC 
CTGTCCCGCC GTGGCCGTCC CCACTGTTCC TGCGATCGTG TCACCTGTGA TGGGTCTTAC 
AGGCCTGTGT GTGCCCAAGA TGGGCACACA TACAACAATG ACTGTTGGCG CCAACAGGCT 
GAGTGTCGAC AACAGCGGGC CATTCCTCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCG 
TCCCCATGCC ATGGAGTGCA ATGTGCATTT GGGGCAGTAT GCACAGTGAA GAACGGGAAA 
GCCGAGTGCG AGTGCCAGCG GGTGTGCTCC GGCATCTACG ATCCTGTGTG CGGCAGTGAC 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCAAGTGG CCCGCAGAGG ACCCTGTGAC CCATGTGGGC AGTGCCGTTT TGGATCCCTG 
TGCGAGGTGG AAACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGCGGTT CTGATGGACA CACATACGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACGCACCAGA TCAGCCTATA CGTGGCCTCA GCTGGACACT GCCAGACCTG TGGAGAAAAA 
GTTTGTACTT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCTCCACCTG GCCCTGTGTG TGGCAGTGAT GGTGTCACCT ACCTCAGTGC CTGTGAGCTA 
CGCGAAGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCATGCAGG GCCATGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGT TCTGGGGAGG ACGACGAGTG TGAACAGGAG 
CTATGCCGGC AGCGTGGTGG TATCTGGGAC GAGGACTCAG AAGATGGGCC ATGTGTCTGT 
GACTTTAGCT GCCAGAGTGT CCCTAGAAGC CCAGTGTGTG GCTCGGATGG AGTCACCTAT 
GGCACCGAGT GTGATCTGAA GAAGGCCAGG TGCGAATCAC AGCAAGAACT GTACGTTGCT 
GCTCAGGGAG CCTGCCGTGG CCCTACCTTG GCTCCACTGC TACCTGTGGC CTTCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATTTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCCCATG GCTCCTACAG TGGCACTTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAACCTG GCTTCTGGAA CTTCCGTGGC ATCGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GCGACCCTCG GGGTGCCGTA AGGGATGACT GTGAACAGAT GACTGGATTG 
TGTTCCTGTA GGCCTGGTGT GGCTGGTCCC AAGTGTGGAC AGTGTCCAGA TGGTCAAGTC 
CTGGGCCATC TAGGCTGTGA AGCAGATCCC ATGACACCTG TGACTTGTGT GGAGATACAC 
TGCGAGTTCG GGGCCTCCTG CGTAGAGAAG GCTGGTTTTG CCCAATGTAT CTGCCCAACC 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGCGGAT CAGACGGTGT GACATATGGC 
AATGAATGCC AGCTGAAGGC CATTGCCTGC CGCCAGCGTC TGGACATCTC CACTCAGAGT 
CTTGGTCCCT GCCAGGAGAG TGTTACTCCT GGGGCTTCCC CAACATCTGC ATCTATGACT 
ACCCCAAGGC ATATCCTGAG CAAGACGCTG CCATTTCCCC ACAACAGCCT TCCTCTGTCT 
CCCGGCAGTA CTACCCATGA TTGGCCCACC CCATTACCCA TATCACCTCA CACCACAGTC 
AGCATCCCCA GAAGCACCGC GTGGCCTGTG CTGACCGTGC CCCCTACAGC AGCAGCCTCT 
GACGTAACCA GTCTCGCAAC ATCAATCTTC AGTGAATCCG GCAGCGCCAA TGGGAGTGGC 
GACGAGGAAC TGAGTGGAGA TGAGGAGGCC AGTGGGGGCG GGTCTGGGGG ACTTGAGCCC 
CCGGTGGGCA GCATTGTGGT GACCCATGGG CCACCCATCG AGAGGGCTTC CTGCTACAAC 
TCACCTTTGG GCTGCTGCTC AGATGGCAAG ACACCCTCAC TGGACTCCGA AGGCTCCAAC 
TGTCCTGCTA CCAAGGCATT CCAGGGCGTG CTGGAGCTTG AGGGGGTCGA GGGACAGGAA 
CTGTTCTACA CACCTGAGAT GGCTGACCCC AAGTCAGAGT TGTTTGGGGA GACCGCAAGG 
AGCATTGAAA GCACGCTGGA CGACCTGTTC CGGAATTCAG ACGTCAAGAA GGACTTCTGG 
AGTGTCCGCC TACGGGAACT GGGGCCTGGC AAGTTGGTCC GCGCCATTGT GGACGTTCAC 
TTTGACCCTA CCACAGCCTT CCAGGCATCA GATGTGGGTC AGGCCTTGCT CCGACAGATC 
CAGGTATCTA GGCCATGGGC CCTGGCAGTG AGGAGGCCTC TGCAGGAACA TGTGCGATTC 
TTAGACTTTG ACTGGTTTCC CACTTTCTTT ACGGGAGCTG CAACAGGAAC TACAGCTGCT 
ATGGCCACAG CCAGAGCCAC CACTGTGAGC CGACTGCCGG CCTCTTCCGT CACCCCACGA 
GTCTACCCCA GTCACACCAG CCGGCCTGTT GGCAGAACTA CAGCACCACC AACCACTCGC 
CGGCCACCAA CCACTGCCAC CAACATGGAC CGGCCTCGGA CCCCAGGCCA TCAACAGCCC 
TCAAAGTCCT GTGATTCCCA ACCTTGCCTC CATGGAGGTA CCTGCCAGGA CCAGGATTCT 
GGCAAGGGTT TCACCTGCAG CTGTACTGCA GGCAGGGGTG GCTCTGTCTG TGAGAAAGTA 
CAACCCCCCT CCATGCCAGC TTTTAAGGGC CACTCCTTCT TGGCCTTCCC CACCCTCCGA 
GCCTACCACA CACTGCGCCT AGCACTGGAA TTCCGGGCTT TGGAGACAGA GGGACTGCTA 
CTCTACAATG GCAATGCACG TGGCAAAGAT TTCCTGGCTC TGGCTCTGTT GGATGGGCGT 
GTGCAGTTCA GGTTTGACAC AGGCTCAGGG CCAGCAGTGC TAACGAGCTT AGTGCCAGTG 
GAACCAGGAC GGTGGCACCG CCTCGAGTTG TCACGGCATT GGCGTCAGGG CACACTTTCT 
GTGGATGGCG AGACTCCTGT TGTAGGTGAA AGTCCCAGTG GCACCGATGG CCTCAACTTG 
GACACGAACC TCTATGTGGG TGGTATCCCA GAAGAACAAG TTGCCATGGT GCTTGATCGG 
ACCTCTGTGG GCGTTGGCCT GAAAGGATGC ATTCGTATGC TGGATATCAA CAATCAGCAG 
CTGGAGCTTA GCGACTGGCA GAGGGCTGCG GTTCAAAGCT CTGGTGTGGG AGAATGTGGA 
GACCATCCGT GCTTACCTAA CCCCTGCCAT GGTGGGGCCC TATGCCAGGC ACTGGAGGCT 
GGCATGTTCC TCTGTCAGTG CCCGCCTGGC CGCTTTGGCC CAACTTGTGC AGATGAAAAG 
AGCCCCTGCC AGCCGAACCC CTGCCATGGG GCAGCCCCCT GTCGTGTGCT TTCCAGCGGT 
GGGGCCAAGT GCGAGTGCCC CCTGGGACGC AGTGGTACCT TCTGTCAGAC AGTCCTGGAG 
ACTGCTGGCT CCCGGCCCTT CCTGGCTGAC TTTAATGGCT TCTCCTACCT GGAACTGAAA 
GGCTTGCACA CCTTCGAGAG AGACCTAGGG GAGAAGATGG CGCTGGAGAT GGTGTTCTTG 
GCTCGTGGGC CCAGTGGCTT ACTCCTCTAC AATGGGCAGA AGACAGATGG CAAGGGGGAC 
TTTGTATCCC TGGCCCTGCA TAACCGGCAC CTAGAGTTCT GCTATGACCT TGGCAAGGGG 
GCTGCAGTCA TCAGGAGCAA AGAGCCCATA GCCCTGGGAA CCTGGGTCAG GGTATTCCTG 
GAACGAAATG GCCGCAAGGG TGCCCTTCAA GTGGGTGATG GTCCCCGTGT GCTAGGGGAA 
TCTCCGAAAT CCCGCAAGGT TCCGCACACC ATGCTCAACC TCAAGGAGCC TCTCTATATT 
GGGGGAGCTC CTGACTTCAG CAAGCTGGCC AGGGGGGCTG CAGTGTCCTC TGGCTTCAGT 
GGTGTCATCC AGCTGGTGTC TCTAAGAGGC CATCAACTGC TGACTCAGGA GCATGTGTTG 
CGGGCAGTAG ATGTCTCACC TTTTGCAGAC CACCCTTGTA CCCAGGCCTT GGGCAACCCC 
TGCCTTAATG GGGGCTCGTG TGTCCCGAGG GAAGCCACTT ATGAATGCCT GTGTCCTGGG 
GGCTTCTCTG GGCTGCACTG CGAAAAGGGG CTAGTTGAGA AGTCAGTGGG GGACCTAGAA 
ACACTGGCCT TTGATGGGCG GACCTACATC GAATACCTCA ATGCTGTGAT TGAGAGTGAG 
AAGGCACTGC AGAGCAACCA CTTTGAACTG AGCTTACGCA CTGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA TTGGCAAGGC TGCAGAACGT GCAGATTACA TGGCCCTGGC CATCGTGGAC 
GGGCACCTGC AACTGAGCTA TGACCTAGGT TCCCAGCCAG TTGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAACCGCTG GCTTCGAATC AGGGCTCACA GGGAGCACAG GGAAGGTTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGATCTTCCC CATTGGGTGC CACGCAACTG 
GACACAGATG GAGCCCTGTG GCTTGGAGGC CTGCAGAAGC TTCCTGTGGG GCAGGCTCTA 
CCCAAGGCCT ATGGCACAGG TTTTGTGGGC TGTCTGCGGG ACGTGGTAGT GGGCCATCGC 
CAGTTGCATC TGCTGGAGGA CGCTGTCACC AAACCAGAGC TAAGACCCTG CCCCACTCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_786930.1
CDS	202..6024
Translation	MPPLPLEHRPRQEPGASMLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	NM_175754.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agrin (Agrn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175754.1

ORF Insert Sequence:

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGGAGC CTGGTGCCTC CATGCTGGTT 
CGATACTTCA TGATCCCCTG CAACATCTGC TTGATCCTGT TGGCCACTTC CACATTGGGC 
TTTGCGGTTC TGCTTTTCCT CAGCAACTAC AAACCTGGAA TCCACTTCAC ACCAGCGCCT 
CCTACGCCTC CTGATGTATG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG TGCCTCCTGT GTGTGCAAGA AGAATGCTTG CCCTGCTACG 
GTGGCTCCTG TGTGTGGCTC AGATGCCTCC ACCTATAGCA ACGAGTGTGA GCTGCAGCGC 
GCGCAGTGCA ACCAGCAACG GCGCATCCGC CTGCTTCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAACGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC GGCTGACGGA 
CAGACTGCCT CATGTCTGTG TCCTACAACC TGCTTCGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGACG GTGTTGACTA CCCTAGTGAA TGCCAGCTGC TTAGTCATGC CTGTGCCAGC 
CAGGAGCACA TCTTCAAGAA GTTCAATGGT CCTTGTGATC CCTGCCAGGG CAGCATGTCA 
GACCTGAACC ACATTTGCCG TGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCTGCCCA GCACACGCCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA ACCCGTGGCC TGCTTCTCCA GAAAGTACGC 
TCTGGTCAAT GCCAAACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCCGTATGC 
CTGTCCCGCC GTGGCCGTCC CCACTGTTCC TGCGATCGTG TCACCTGTGA TGGGTCTTAC 
AGGCCTGTGT GTGCCCAAGA TGGGCACACA TACAACAATG ACTGTTGGCG CCAACAGGCT 
GAGTGTCGAC AACAGCGGGC CATTCCTCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCG 
TCCCCATGCC ATGGAGTGCA ATGTGCATTT GGGGCAGTAT GCACAGTGAA GAACGGGAAA 
GCCGAGTGCG AGTGCCAGCG GGTGTGCTCC GGCATCTACG ATCCTGTGTG CGGCAGTGAC 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCAAGTGG CCCGCAGAGG ACCCTGTGAC CCATGTGGGC AGTGCCGTTT TGGATCCCTG 
TGCGAGGTGG AAACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGCGGTT CTGATGGACA CACATACGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACGCACCAGA TCAGCCTATA CGTGGCCTCA GCTGGACACT GCCAGACCTG TGGAGAAAAA 
GTTTGTACTT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCTCCACCTG GCCCTGTGTG TGGCAGTGAT GGTGTCACCT ACCTCAGTGC CTGTGAGCTA 
CGCGAAGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCATGCAGG GCCATGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGT TCTGGGGAGG ACGACGAGTG TGAACAGGAG 
CTATGCCGGC AGCGTGGTGG TATCTGGGAC GAGGACTCAG AAGATGGGCC ATGTGTCTGT 
GACTTTAGCT GCCAGAGTGT CCCTAGAAGC CCAGTGTGTG GCTCGGATGG AGTCACCTAT 
GGCACCGAGT GTGATCTGAA GAAGGCCAGG TGCGAATCAC AGCAAGAACT GTACGTTGCT 
GCTCAGGGAG CCTGCCGTGG CCCTACCTTG GCTCCACTGC TACCTGTGGC CTTCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATTTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCCCATG GCTCCTACAG TGGCACTTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAACCTG GCTTCTGGAA CTTCCGTGGC ATCGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GCGACCCTCG GGGTGCCGTA AGGGATGACT GTGAACAGAT GACTGGATTG 
TGTTCCTGTA GGCCTGGTGT GGCTGGTCCC AAGTGTGGAC AGTGTCCAGA TGGTCAAGTC 
CTGGGCCATC TAGGCTGTGA AGCAGATCCC ATGACACCTG TGACTTGTGT GGAGATACAC 
TGCGAGTTCG GGGCCTCCTG CGTAGAGAAG GCTGGTTTTG CCCAATGTAT CTGCCCAACC 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGCGGAT CAGACGGTGT GACATATGGC 
AATGAATGCC AGCTGAAGGC CATTGCCTGC CGCCAGCGTC TGGACATCTC CACTCAGAGT 
CTTGGTCCCT GCCAGGAGAG TGTTACTCCT GGGGCTTCCC CAACATCTGC ATCTATGACT 
ACCCCAAGGC ATATCCTGAG CAAGACGCTG CCATTTCCCC ACAACAGCCT TCCTCTGTCT 
CCCGGCAGTA CTACCCATGA TTGGCCCACC CCATTACCCA TATCACCTCA CACCACAGTC 
AGCATCCCCA GAAGCACCGC GTGGCCTGTG CTGACCGTGC CCCCTACAGC AGCAGCCTCT 
GACGTAACCA GTCTCGCAAC ATCAATCTTC AGTGAATCCG GCAGCGCCAA TGGGAGTGGC 
GACGAGGAAC TGAGTGGAGA TGAGGAGGCC AGTGGGGGCG GGTCTGGGGG ACTTGAGCCC 
CCGGTGGGCA GCATTGTGGT GACCCATGGG CCACCCATCG AGAGGGCTTC CTGCTACAAC 
TCACCTTTGG GCTGCTGCTC AGATGGCAAG ACACCCTCAC TGGACTCCGA AGGCTCCAAC 
TGTCCTGCTA CCAAGGCATT CCAGGGCGTG CTGGAGCTTG AGGGGGTCGA GGGACAGGAA 
CTGTTCTACA CACCTGAGAT GGCTGACCCC AAGTCAGAGT TGTTTGGGGA GACCGCAAGG 
AGCATTGAAA GCACGCTGGA CGACCTGTTC CGGAATTCAG ACGTCAAGAA GGACTTCTGG 
AGTGTCCGCC TACGGGAACT GGGGCCTGGC AAGTTGGTCC GCGCCATTGT GGACGTTCAC 
TTTGACCCTA CCACAGCCTT CCAGGCATCA GATGTGGGTC AGGCCTTGCT CCGACAGATC 
CAGGTATCTA GGCCATGGGC CCTGGCAGTG AGGAGGCCTC TGCAGGAACA TGTGCGATTC 
TTAGACTTTG ACTGGTTTCC CACTTTCTTT ACGGGAGCTG CAACAGGAAC TACAGCTGCT 
ATGGCCACAG CCAGAGCCAC CACTGTGAGC CGACTGCCGG CCTCTTCCGT CACCCCACGA 
GTCTACCCCA GTCACACCAG CCGGCCTGTT GGCAGAACTA CAGCACCACC AACCACTCGC 
CGGCCACCAA CCACTGCCAC CAACATGGAC CGGCCTCGGA CCCCAGGCCA TCAACAGCCC 
TCAAAGTCCT GTGATTCCCA ACCTTGCCTC CATGGAGGTA CCTGCCAGGA CCAGGATTCT 
GGCAAGGGTT TCACCTGCAG CTGTACTGCA GGCAGGGGTG GCTCTGTCTG TGAGAAAGTA 
CAACCCCCCT CCATGCCAGC TTTTAAGGGC CACTCCTTCT TGGCCTTCCC CACCCTCCGA 
GCCTACCACA CACTGCGCCT AGCACTGGAA TTCCGGGCTT TGGAGACAGA GGGACTGCTA 
CTCTACAATG GCAATGCACG TGGCAAAGAT TTCCTGGCTC TGGCTCTGTT GGATGGGCGT 
GTGCAGTTCA GGTTTGACAC AGGCTCAGGG CCAGCAGTGC TAACGAGCTT AGTGCCAGTG 
GAACCAGGAC GGTGGCACCG CCTCGAGTTG TCACGGCATT GGCGTCAGGG CACACTTTCT 
GTGGATGGCG AGACTCCTGT TGTAGGTGAA AGTCCCAGTG GCACCGATGG CCTCAACTTG 
GACACGAACC TCTATGTGGG TGGTATCCCA GAAGAACAAG TTGCCATGGT GCTTGATCGG 
ACCTCTGTGG GCGTTGGCCT GAAAGGATGC ATTCGTATGC TGGATATCAA CAATCAGCAG 
CTGGAGCTTA GCGACTGGCA GAGGGCTGCG GTTCAAAGCT CTGGTGTGGG AGAATGTGGA 
GACCATCCGT GCTTACCTAA CCCCTGCCAT GGTGGGGCCC TATGCCAGGC ACTGGAGGCT 
GGCATGTTCC TCTGTCAGTG CCCGCCTGGC CGCTTTGGCC CAACTTGTGC AGATGAAAAG 
AGCCCCTGCC AGCCGAACCC CTGCCATGGG GCAGCCCCCT GTCGTGTGCT TTCCAGCGGT 
GGGGCCAAGT GCGAGTGCCC CCTGGGACGC AGTGGTACCT TCTGTCAGAC AGTCCTGGAG 
ACTGCTGGCT CCCGGCCCTT CCTGGCTGAC TTTAATGGCT TCTCCTACCT GGAACTGAAA 
GGCTTGCACA CCTTCGAGAG AGACCTAGGG GAGAAGATGG CGCTGGAGAT GGTGTTCTTG 
GCTCGTGGGC CCAGTGGCTT ACTCCTCTAC AATGGGCAGA AGACAGATGG CAAGGGGGAC 
TTTGTATCCC TGGCCCTGCA TAACCGGCAC CTAGAGTTCT GCTATGACCT TGGCAAGGGG 
GCTGCAGTCA TCAGGAGCAA AGAGCCCATA GCCCTGGGAA CCTGGGTCAG GGTATTCCTG 
GAACGAAATG GCCGCAAGGG TGCCCTTCAA GTGGGTGATG GTCCCCGTGT GCTAGGGGAA 
TCTCCGAAAT CCCGCAAGGT TCCGCACACC ATGCTCAACC TCAAGGAGCC TCTCTATATT 
GGGGGAGCTC CTGACTTCAG CAAGCTGGCC AGGGGGGCTG CAGTGTCCTC TGGCTTCAGT 
GGTGTCATCC AGCTGGTGTC TCTAAGAGGC CATCAACTGC TGACTCAGGA GCATGTGTTG 
CGGGCAGTAG ATGTCTCACC TTTTGCAGAC CACCCTTGTA CCCAGGCCTT GGGCAACCCC 
TGCCTTAATG GGGGCTCGTG TGTCCCGAGG GAAGCCACTT ATGAATGCCT GTGTCCTGGG 
GGCTTCTCTG GGCTGCACTG CGAAAAGGGG CTAGTTGAGA AGTCAGTGGG GGACCTAGAA 
ACACTGGCCT TTGATGGGCG GACCTACATC GAATACCTCA ATGCTGTGAT TGAGAGTGAG 
AAGGCACTGC AGAGCAACCA CTTTGAACTG AGCTTACGCA CTGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA TTGGCAAGGC TGCAGAACGT GCAGATTACA TGGCCCTGGC CATCGTGGAC 
GGGCACCTGC AACTGAGCTA TGACCTAGGT TCCCAGCCAG TTGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAACCGCTG GCTTCGAATC AGGGCTCACA GGGAGCACAG GGAAGGTTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGATCTTCCC CATTGGGTGC CACGCAACTG 
GACACAGATG GAGCCCTGTG GCTTGGAGGC CTGCAGAAGC TTCCTGTGGG GCAGGCTCTA 
CCCAAGGCCT ATGGCACAGG TTTTGTGGGC TGTCTGCGGG ACGTGGTAGT GGGCCATCGC 
CAGTTGCATC TGCTGGAGGA CGCTGTCACC AAACCAGAGC TAAGACCCTG CCCCACTCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_017593170.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448659.1 Rattus norvegicus agrin (Agrn), transcript variant X7, mRNA.

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CloneID	ORa61112
Clone ID Related Accession (Same CDS sequence)	XM_017593170.1
Accession Version	XM_017593170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	agrin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGGAGC CTGGTGCCTC CATGCTGGTT 
CGATACTTCA TGATCCCCTG CAACATCTGC TTGATCCTGT TGGCCACTTC CACATTGGGC 
TTTGCGGTTC TGCTTTTCCT CAGCAACTAC AAACCTGGAA TCCACTTCAC ACCAGCGCCT 
CCTACGCCTC CTGATGTATG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG TGCCTCCTGT GTGTGCAAGA AGAATGCTTG CCCTGCTACG 
GTGGCTCCTG TGTGTGGCTC AGATGCCTCC ACCTATAGCA ACGAGTGCGA GCTGCAGCGC 
GCGCAGTGCA ACCAGCAACG GCGCATCCGC CTGCTTCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAACGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC GGCTGACGGA 
CAGACTGCCT CATGTCTGTG TCCTACAACC TGCTTCGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGACG GTGTTGACTA CCCTAGTGAA TGCCAGCTGC TTAGTCATGC CTGTGCCAGC 
CAGGAGCACA TCTTCAAGAA GTTCAATGGT CCTTGTGATC CCTGCCAGGG CAGCATGTCA 
GACCTGAACC ACATTTGCCG TGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCTGCCCA GCACACGCCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA ACCCGTGGCC TGCTTCTCCA GAAAGTACGC 
TCTGGTCAAT GCCAAACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCCGTATGC 
CTGTCCCGCC GTGGCCGTCC CCACTGTTCC TGCGATCGTG TCACCTGTGA TGGGTCTTAC 
AGGCCTGTGT GTGCCCAAGA TGGGCACACA TACAACAATG ACTGTTGGCG CCAACAGGCT 
GAGTGTCGAC AACAGCGGGC CATTCCTCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCG 
TCCCCATGCC ATGGAGTGCA ATGTGCATTT GGGGCAGTAT GCACAGTGAA GAACGGGAAA 
GCCGAGTGTG AGTGCCAGCG GGTGTGCTCC GGCATCTACG ATCCTGTGTG CGGCAGTGAC 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCAAGTGG CCCGCAGAGG ACCCTGTGAC CCATGTGGGC AGTGCCGTTT TGGATCCCTG 
TGCGAGGTGG AAACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGCGGTT CTGATGGACA CACATACGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACGCACCAGA TCAGCCTATA CGTGGCCTCA GCTGGACACT GCCAGACCTG TGGAGAAAAA 
GTTTGTACTT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCTCCACCTG GCCCTGTGTG TGGCAGTGAT GGTGTCACCT ACCTCAGTGC CTGTGAGCTA 
CGCGAAGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCATGCAGG GCCATGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGT TCTGGGGAGG ACGACGAGTG TGAACAGGAG 
CTATGCCGGC AGCGTGGTGG TATCTGGGAC GAGGACTCAG AAGATGGGCC ATGTGTCTGT 
GACTTTAGCT GCCAGAGTGT CCCTAGAAGC CCAGTGTGTG GCTCGGATGG AGTCACCTAT 
GGCACCGAGT GTGATCTGAA GAAGGCCAGG TGCGAATCAC AGCAAGAACT GTACGTTGCT 
GCTCAGGGAG CCTGCCGTGG CCCTACCTTG GCTCCACTGC TACCTGTGGC CTTCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATTTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCCCATG GCTCCTACAG TGGCACTTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAACCTG GCTTCTGGAA CTTCCGTGGC ATCGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GCGACCCTCG GGGTGCCGTA AGGGATGACT GTGAACAGAT GACTGGATTG 
TGTTCCTGTA GGCCTGGTGT GGCTGGTCCC AAGTGTGGAC AGTGTCCAGA TGGTCAAGTC 
CTGGGCCATC TAGGCTGTGA AGCAGATCCC ATGACACCTG TGACTTGTGT GGAGATACAC 
TGCGAGTTCG GGGCCTCCTG CGTAGAGAAG GCTGGTTTTG CCCAATGTAT CTGCCCAACC 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGCGGAT CAGACGGTGT GACATATGGC 
AATGAATGCC AGCTGAAGGC CATTGCCTGC CGCCAGCGTC TGGACATCTC CACTCAGAGT 
CTTGGTCCCT GCCAGGAGAG TGTTACTCCT GGGGCTTCCC CAACATCTGC ATCTATGACT 
ACCCCAAGGC ATATCCTGAG CAAGACGCTG CCATTTCCCC ACAACAGCCT TCCTCTGTCT 
CCCGGCAGTA CTACCCATGA TTGGCCCACC CCATTACCCA TATCACCTCA CACCACAGTC 
AGCATCCCCA GAAGCACCGC GTGGCCTGTG CTGACCGTGC CCCCTACAGC AGCAGCCTCT 
GACGTAACCA GTCTCGCAAC ATCAATCTTC AGTGAATCCG GCAGCGCCAA TGGGAGTGGC 
GACGAGGAAC TGAGTGGAGA TGAGGAGGCC AGTGGGGGCG GGTCTGGGGG ACTTGAGCCC 
CCGGTGGGCA GCATTGTGGT GACCCATGGG CCACCCATCG AGAGGGCTTC CTGCTACAAC 
TCACCTTTGG GCTGCTGCTC AGATGGCAAG ACACCCTCAC TGGACTCCGA AGGCTCCAAC 
TGTCCTGCTA CCAAGGCATT CCAGGGCGTG CTGGAGCTTG AGGGGGTCGA GGGACAGGAA 
CTGTTCTACA CACCTGAGAT GGCTGACCCC AAGTCAGAGT TGTTTGGGGA GACCGCAAGG 
AGCATTGAAA GCACGCTGGA CGACCTGTTC CGGAATTCAG ACGTCAAGAA GGACTTCTGG 
AGTGTCCGCC TACGGGAACT GGGGCCTGGC AAGTTGGTCC GCGCCATTGT GGACGTTCAC 
TTTGACCCTA CCACAGCCTT CCAGGCATCA GATGTGGGTC AGGCCTTGCT CCGACAGATC 
CAGGTATCTA GGCCATGGGC CCTGGCAGTG AGGAGGCCTC TGCAGGAACA TGTGCGATTC 
TTAGACTTTG ACTGGTTTCC CACTTTCTTT ACGGGAGCTG CAACAGGAAC TACAGCTGCT 
ATGGCCACAG CCAGAGCCAC CACTGTGAGC CGACTGCCGG CCTCTTCCGT CACCCCACGA 
GTCTACCCCA GTCACACCAG CCGGCCTGTT GGCAGAACTA CAGCACCACC AACCACTCGC 
CGGCCACCAA CCACTGCCAC CAACATGGAC CGGCCTCGGA CCCCAGGCCA TCAACAGCCC 
TCAAAGTCCT GTGATTCCCA ACCTTGCCTC CATGGAGGTA CCTGCCAGGA CCAGGATTCT 
GGCAAGGGTT TCACCTGCAG CTGTACTGCA GGCAGGGGTG GCTCTGTCTG TGAGAAAGTA 
CAACCCCCCT CCATGCCAGC TTTTAAGGGC CACTCCTTCT TGGCCTTCCC CACCCTCCGA 
GCCTACCACA CACTGCGCCT AGCACTGGAA TTCCGGGCTT TGGAGACAGA GGGACTGCTA 
CTCTACAATG GCAATGCACG TGGCAAAGAT TTCCTGGCTC TGGCTCTGTT GGATGGGCGT 
GTGCAGTTCA GGTTTGACAC AGGCTCAGGG CCAGCAGTGC TAACGAGCTT AGTGCCAGTG 
GAACCAGGAC GGTGGCACCG CCTCGAGTTG TCACGGCATT GGCGTCAGGG CACACTTTCT 
GTGGATGGCG AGACTCCTGT TGTAGGTGAA AGTCCCAGTG GCACCGATGG CCTCAACTTG 
GACACGAACC TCTATGTGGG TGGTATCCCA GAAGAACAAG TTGCCATGGT GCTTGATCGG 
ACCTCTGTGG GCGTTGGCCT GAAAGGATGC ATTCGTATGC TGGATATCAA CAATCAGCAG 
CTGGAGCTTA GCGACTGGCA GAGGGCTGCG GTTCAAAGCT CTGGTGTGGG AGAATGTGGA 
GACCATCCGT GCTTACCTAA CCCCTGCCAT GGTGGGGCCC TATGCCAGGC ACTGGAGGCT 
GGCATGTTCC TCTGTCAGTG CCCGCCTGGC CGCTTTGGCC CAACTTGTGC AGATGAAAAG 
AGCCCCTGCC AGCCGAACCC CTGCCATGGG GCAGCCCCCT GTCGTGTGCT TTCCAGCGGT 
GGGGCCAAGT GCGAGTGCCC CCTGGGACGC AGTGGTACCT TCTGTCAGAC AGTCCTGGAG 
ACTGCTGGCT CCCGGCCCTT CCTGGCTGAC TTTAATGGCT TCTCCTACCT GGAACTGAAA 
GGCTTGCACA CCTTCGAGAG AGACCTAGGG GAGAAGATGG CGCTGGAGAT GGTGTTCTTG 
GCTCGTGGGC CCAGTGGCTT ACTCCTCTAC AATGGGCAGA AGACAGATGG CAAGGGGGAC 
TTTGTATCCC TGGCCCTGCA TAACCGGCAC CTAGAGTTCT GCTATGACCT TGGCAAGGGG 
GCTGCAGTCA TCAGGAGCAA AGAGCCCATA GCCCTGGGAA CCTGGGTCAG GGTATTCCTG 
GAACGAAATG GCCGCAAGGG TGCCCTTCAA GTGGGTGATG GTCCCCGTGT GCTAGGGGAA 
TCTCCGAAAT CCCGCAAGGT TCCGCACACC ATGCTCAACC TCAAGGAGCC TCTCTATATT 
GGGGGAGCTC CTGACTTCAG CAAGCTGGCC AGGGGGGCTG CAGTGTCCTC TGGCTTCAAT 
GGTGTCATCC AGCTGGTGTC TCTAAGAGGC CATCAACTGC TGACTCAGGA GCATGTGTTG 
CGGGCAGTAG ATGTCTCACC TTTTGCAGAC CACCCTTGTA CCCAGGCCTT GGGCAACCCC 
TGCCTTAATG GGGGCTCGTG TGTCCCGAGG GAAGCCACTT ATGAATGCCT GTGTCCTGGG 
GGCTTCTCTG GGCTGCACTG CGAAAAGGGG CTAGTTGAGA AGTCAGTGGG GGACCTAGAA 
ACACTGGCCT TTGATGGGCG GACCTACATC GAATACCTCA ATGCTGTGAT TGAGAGCGAG 
CTGACCAATG AGATCCCAGC CCCTGAAACT CTGGATTCCC GGGCCCTTTT CAGTGAGAAG 
GCACTGCAGA GCAACCACTT TGAACTGAGC TTACGCACTG AGGCCACTCA GGGGCTGGTG 
CTGTGGATTG GCAAGGCTGC AGAACGTGCA GATTACATGG CCCTGGCCAT CGTGGACGGG 
CACCTGCAAC TGAGCTATGA CCTAGGTTCC CAGCCAGTTG TGCTGCGCTC CACTGTGAAG 
GTCAACACCA ACCGCTGGCT TCGAATCAGG GCTCACAGGG AGCACAGGGA AGGTTCCCTT 
CAGGTGGGCA ATGAAGCCCC TGTGACTGGA TCTTCCCCAT TGGGTGCCAC GCAACTGGAC 
ACAGATGGAG CCCTGTGGCT TGGAGGCCTG CAGAAGCTTC CTGTGGGGCA GGCTCTACCC 
AAGGCCTATG GCACAGGTTT TGTGGGCTGT CTGCGGGACG TGGTAGTGGG CCATCGCCAG 
TTGCATCTGC TGGAGGACGC TGTCACCAAA CCAGAGCTAA GACCCTGCCC CACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448659.1
CDS	1677..7556
Translation	MPPLPLEHRPRQEPGASMLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFNGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	XM_017593170.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agrin (Agrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593170.1

ORF Insert Sequence:

ATGCCTCCTC TGCCACTGGA ACACAGACCC AGGCAGGAGC CTGGTGCCTC CATGCTGGTT 
CGATACTTCA TGATCCCCTG CAACATCTGC TTGATCCTGT TGGCCACTTC CACATTGGGC 
TTTGCGGTTC TGCTTTTCCT CAGCAACTAC AAACCTGGAA TCCACTTCAC ACCAGCGCCT 
CCTACGCCTC CTGATGTATG CAGGGGAATG TTATGTGGCT TTGGTGCTGT GTGTGAACCT 
AGTGTTGAGG ATCCAGGCCG TGCCTCCTGT GTGTGCAAGA AGAATGCTTG CCCTGCTACG 
GTGGCTCCTG TGTGTGGCTC AGATGCCTCC ACCTATAGCA ACGAGTGCGA GCTGCAGCGC 
GCGCAGTGCA ACCAGCAACG GCGCATCCGC CTGCTTCGCC AAGGGCCATG TGGGTCCCGG 
GACCCCTGTG CCAACGTGAC CTGCAGTTTC GGTAGTACCT GTGTACCTTC GGCTGACGGA 
CAGACTGCCT CATGTCTGTG TCCTACAACC TGCTTCGGGG CCCCTGATGG CACAGTGTGT 
GGCAGTGACG GTGTTGACTA CCCTAGTGAA TGCCAGCTGC TTAGTCATGC CTGTGCCAGC 
CAGGAGCACA TCTTCAAGAA GTTCAATGGT CCTTGTGATC CCTGCCAGGG CAGCATGTCA 
GACCTGAACC ACATTTGCCG TGTGAACCCA CGTACACGGC ACCCAGAAAT GCTTCTGCGG 
CCTGAGAACT GCCCTGCCCA GCACACGCCT ATCTGTGGAG ATGATGGGGT CACCTATGAA 
AACGACTGTG TCATGAGCCG TATAGGTGCA ACCCGTGGCC TGCTTCTCCA GAAAGTACGC 
TCTGGTCAAT GCCAAACTCG AGACCAGTGC CCGGAGACCT GCCAGTTTAA CTCCGTATGC 
CTGTCCCGCC GTGGCCGTCC CCACTGTTCC TGCGATCGTG TCACCTGTGA TGGGTCTTAC 
AGGCCTGTGT GTGCCCAAGA TGGGCACACA TACAACAATG ACTGTTGGCG CCAACAGGCT 
GAGTGTCGAC AACAGCGGGC CATTCCTCCC AAGCACCAGG GCCCGTGTGA CCAGACCCCG 
TCCCCATGCC ATGGAGTGCA ATGTGCATTT GGGGCAGTAT GCACAGTGAA GAACGGGAAA 
GCCGAGTGTG AGTGCCAGCG GGTGTGCTCC GGCATCTACG ATCCTGTGTG CGGCAGTGAC 
GGTGTCACTT ACGGCAGTGT GTGCGAGCTG GAATCCATGG CCTGTACCCT TGGGCGGGAA 
ATCCAAGTGG CCCGCAGAGG ACCCTGTGAC CCATGTGGGC AGTGCCGTTT TGGATCCCTG 
TGCGAGGTGG AAACTGGACG CTGTGTGTGC CCCTCTGAGT GTGTGGAGTC AGCCCAGCCC 
GTATGCGGTT CTGATGGACA CACATACGCT AGTGAATGTG AGCTGCATGT CCACGCCTGT 
ACGCACCAGA TCAGCCTATA CGTGGCCTCA GCTGGACACT GCCAGACCTG TGGAGAAAAA 
GTTTGTACTT TTGGGGCTGT GTGCTCAGCT GGACAGTGTG TATGTCCCCG TTGTGAGCAC 
CCTCCACCTG GCCCTGTGTG TGGCAGTGAT GGTGTCACCT ACCTCAGTGC CTGTGAGCTA 
CGCGAAGCTG CCTGTCAGCA GCAGGTACAA ATTGAGGAGG CCCATGCAGG GCCATGTGAG 
CCGGCTGAGT GTGGCTCAGG GGGCTCTGGT TCTGGGGAGG ACGACGAGTG TGAACAGGAG 
CTATGCCGGC AGCGTGGTGG TATCTGGGAC GAGGACTCAG AAGATGGGCC ATGTGTCTGT 
GACTTTAGCT GCCAGAGTGT CCCTAGAAGC CCAGTGTGTG GCTCGGATGG AGTCACCTAT 
GGCACCGAGT GTGATCTGAA GAAGGCCAGG TGCGAATCAC AGCAAGAACT GTACGTTGCT 
GCTCAGGGAG CCTGCCGTGG CCCTACCTTG GCTCCACTGC TACCTGTGGC CTTCCCACAC 
TGTGCCCAAA CCCCCTATGG CTGCTGCCAG GACAATTTCA CTGCTGCCCA GGGTGTGGGC 
TTGGCTGGCT GTCCCAGCAC CTGCCATTGC AACCCCCATG GCTCCTACAG TGGCACTTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGTGTAG GAGGCCTCAG GTGTGATCGC 
TGTGAACCTG GCTTCTGGAA CTTCCGTGGC ATCGTCACCG ATGGACATAG TGGTTGCACT 
CCCTGCAGCT GCGACCCTCG GGGTGCCGTA AGGGATGACT GTGAACAGAT GACTGGATTG 
TGTTCCTGTA GGCCTGGTGT GGCTGGTCCC AAGTGTGGAC AGTGTCCAGA TGGTCAAGTC 
CTGGGCCATC TAGGCTGTGA AGCAGATCCC ATGACACCTG TGACTTGTGT GGAGATACAC 
TGCGAGTTCG GGGCCTCCTG CGTAGAGAAG GCTGGTTTTG CCCAATGTAT CTGCCCAACC 
CTCACATGTC CAGAGGCTAA CTCTACCAAG GTCTGCGGAT CAGACGGTGT GACATATGGC 
AATGAATGCC AGCTGAAGGC CATTGCCTGC CGCCAGCGTC TGGACATCTC CACTCAGAGT 
CTTGGTCCCT GCCAGGAGAG TGTTACTCCT GGGGCTTCCC CAACATCTGC ATCTATGACT 
ACCCCAAGGC ATATCCTGAG CAAGACGCTG CCATTTCCCC ACAACAGCCT TCCTCTGTCT 
CCCGGCAGTA CTACCCATGA TTGGCCCACC CCATTACCCA TATCACCTCA CACCACAGTC 
AGCATCCCCA GAAGCACCGC GTGGCCTGTG CTGACCGTGC CCCCTACAGC AGCAGCCTCT 
GACGTAACCA GTCTCGCAAC ATCAATCTTC AGTGAATCCG GCAGCGCCAA TGGGAGTGGC 
GACGAGGAAC TGAGTGGAGA TGAGGAGGCC AGTGGGGGCG GGTCTGGGGG ACTTGAGCCC 
CCGGTGGGCA GCATTGTGGT GACCCATGGG CCACCCATCG AGAGGGCTTC CTGCTACAAC 
TCACCTTTGG GCTGCTGCTC AGATGGCAAG ACACCCTCAC TGGACTCCGA AGGCTCCAAC 
TGTCCTGCTA CCAAGGCATT CCAGGGCGTG CTGGAGCTTG AGGGGGTCGA GGGACAGGAA 
CTGTTCTACA CACCTGAGAT GGCTGACCCC AAGTCAGAGT TGTTTGGGGA GACCGCAAGG 
AGCATTGAAA GCACGCTGGA CGACCTGTTC CGGAATTCAG ACGTCAAGAA GGACTTCTGG 
AGTGTCCGCC TACGGGAACT GGGGCCTGGC AAGTTGGTCC GCGCCATTGT GGACGTTCAC 
TTTGACCCTA CCACAGCCTT CCAGGCATCA GATGTGGGTC AGGCCTTGCT CCGACAGATC 
CAGGTATCTA GGCCATGGGC CCTGGCAGTG AGGAGGCCTC TGCAGGAACA TGTGCGATTC 
TTAGACTTTG ACTGGTTTCC CACTTTCTTT ACGGGAGCTG CAACAGGAAC TACAGCTGCT 
ATGGCCACAG CCAGAGCCAC CACTGTGAGC CGACTGCCGG CCTCTTCCGT CACCCCACGA 
GTCTACCCCA GTCACACCAG CCGGCCTGTT GGCAGAACTA CAGCACCACC AACCACTCGC 
CGGCCACCAA CCACTGCCAC CAACATGGAC CGGCCTCGGA CCCCAGGCCA TCAACAGCCC 
TCAAAGTCCT GTGATTCCCA ACCTTGCCTC CATGGAGGTA CCTGCCAGGA CCAGGATTCT 
GGCAAGGGTT TCACCTGCAG CTGTACTGCA GGCAGGGGTG GCTCTGTCTG TGAGAAAGTA 
CAACCCCCCT CCATGCCAGC TTTTAAGGGC CACTCCTTCT TGGCCTTCCC CACCCTCCGA 
GCCTACCACA CACTGCGCCT AGCACTGGAA TTCCGGGCTT TGGAGACAGA GGGACTGCTA 
CTCTACAATG GCAATGCACG TGGCAAAGAT TTCCTGGCTC TGGCTCTGTT GGATGGGCGT 
GTGCAGTTCA GGTTTGACAC AGGCTCAGGG CCAGCAGTGC TAACGAGCTT AGTGCCAGTG 
GAACCAGGAC GGTGGCACCG CCTCGAGTTG TCACGGCATT GGCGTCAGGG CACACTTTCT 
GTGGATGGCG AGACTCCTGT TGTAGGTGAA AGTCCCAGTG GCACCGATGG CCTCAACTTG 
GACACGAACC TCTATGTGGG TGGTATCCCA GAAGAACAAG TTGCCATGGT GCTTGATCGG 
ACCTCTGTGG GCGTTGGCCT GAAAGGATGC ATTCGTATGC TGGATATCAA CAATCAGCAG 
CTGGAGCTTA GCGACTGGCA GAGGGCTGCG GTTCAAAGCT CTGGTGTGGG AGAATGTGGA 
GACCATCCGT GCTTACCTAA CCCCTGCCAT GGTGGGGCCC TATGCCAGGC ACTGGAGGCT 
GGCATGTTCC TCTGTCAGTG CCCGCCTGGC CGCTTTGGCC CAACTTGTGC AGATGAAAAG 
AGCCCCTGCC AGCCGAACCC CTGCCATGGG GCAGCCCCCT GTCGTGTGCT TTCCAGCGGT 
GGGGCCAAGT GCGAGTGCCC CCTGGGACGC AGTGGTACCT TCTGTCAGAC AGTCCTGGAG 
ACTGCTGGCT CCCGGCCCTT CCTGGCTGAC TTTAATGGCT TCTCCTACCT GGAACTGAAA 
GGCTTGCACA CCTTCGAGAG AGACCTAGGG GAGAAGATGG CGCTGGAGAT GGTGTTCTTG 
GCTCGTGGGC CCAGTGGCTT ACTCCTCTAC AATGGGCAGA AGACAGATGG CAAGGGGGAC 
TTTGTATCCC TGGCCCTGCA TAACCGGCAC CTAGAGTTCT GCTATGACCT TGGCAAGGGG 
GCTGCAGTCA TCAGGAGCAA AGAGCCCATA GCCCTGGGAA CCTGGGTCAG GGTATTCCTG 
GAACGAAATG GCCGCAAGGG TGCCCTTCAA GTGGGTGATG GTCCCCGTGT GCTAGGGGAA 
TCTCCGAAAT CCCGCAAGGT TCCGCACACC ATGCTCAACC TCAAGGAGCC TCTCTATATT 
GGGGGAGCTC CTGACTTCAG CAAGCTGGCC AGGGGGGCTG CAGTGTCCTC TGGCTTCAAT 
GGTGTCATCC AGCTGGTGTC TCTAAGAGGC CATCAACTGC TGACTCAGGA GCATGTGTTG 
CGGGCAGTAG ATGTCTCACC TTTTGCAGAC CACCCTTGTA CCCAGGCCTT GGGCAACCCC 
TGCCTTAATG GGGGCTCGTG TGTCCCGAGG GAAGCCACTT ATGAATGCCT GTGTCCTGGG 
GGCTTCTCTG GGCTGCACTG CGAAAAGGGG CTAGTTGAGA AGTCAGTGGG GGACCTAGAA 
ACACTGGCCT TTGATGGGCG GACCTACATC GAATACCTCA ATGCTGTGAT TGAGAGCGAG 
CTGACCAATG AGATCCCAGC CCCTGAAACT CTGGATTCCC GGGCCCTTTT CAGTGAGAAG 
GCACTGCAGA GCAACCACTT TGAACTGAGC TTACGCACTG AGGCCACTCA GGGGCTGGTG 
CTGTGGATTG GCAAGGCTGC AGAACGTGCA GATTACATGG CCCTGGCCAT CGTGGACGGG 
CACCTGCAAC TGAGCTATGA CCTAGGTTCC CAGCCAGTTG TGCTGCGCTC CACTGTGAAG 
GTCAACACCA ACCGCTGGCT TCGAATCAGG GCTCACAGGG AGCACAGGGA AGGTTCCCTT 
CAGGTGGGCA ATGAAGCCCC TGTGACTGGA TCTTCCCCAT TGGGTGCCAC GCAACTGGAC 
ACAGATGGAG CCCTGTGGCT TGGAGGCCTG CAGAAGCTTC CTGTGGGGCA GGCTCTACCC 
AAGGCCTATG GCACAGGTTT TGTGGGCTGT CTGCGGGACG TGGTAGTGGG CCATCGCCAG 
TTGCATCTGC TGGAGGACGC TGTCACCAAA CCAGAGCTAA GACCCTGCCC CACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006239541.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239603.1 Rattus norvegicus agrin (Agrn), transcript variant X5, mRNA.

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CloneID	ORa28987
Clone ID Related Accession (Same CDS sequence)	XM_006239541.3
Accession Version	XM_006239541.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239541.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGAAA TCCCGCAAGG TTCCGCACAC CATGCTCAAC 
CTCAAGGAGC CTCTCTATAT TGGGGGAGCT CCTGACTTCA GCAAGCTGGC CAGGGGGGCT 
GCAGTGTCCT CTGGCTTCAA TGGTGTCATC CAGCTGGTGT CTCTAAGAGG CCATCAACTG 
CTGACTCAGG AGCATGTGTT GCGGGCAGTA GATGTCTCAC CTTTTGCAGA CCACCCTTGT 
ACCCAGGCCT TGGGCAACCC CTGCCTTAAT GGGGGCTCGT GTGTCCCGAG GGAAGCCACT 
TATGAATGCC TGTGTCCTGG GGGCTTCTCT GGGCTGCACT GCGAAAAGGG GCTAGTTGAG 
AAGTCAGTGG GGGACCTAGA AACACTGGCC TTTGATGGGC GGACCTACAT CGAATACCTC 
AATGCTGTGA TTGAGAGTGA GAAGGCACTG CAGAGCAACC ACTTTGAACT GAGCTTACGC 
ACTGAGGCCA CTCAGGGGCT GGTGCTGTGG ATTGGCAAGG CTGCAGAACG TGCAGATTAC 
ATGGCCCTGG CCATCGTGGA CGGGCACCTG CAACTGAGCT ATGACCTAGG TTCCCAGCCA 
GTTGTGCTGC GCTCCACTGT GAAGGTCAAC ACCAACCGCT GGCTTCGAAT CAGGGCTCAC 
AGGGAGCACA GGGAAGGTTC CCTTCAGGTG GGCAATGAAG CCCCTGTGAC TGGATCTTCC 
CCATTGGGTG CCACGCAACT GGACACAGAT GGAGCCCTGT GGCTTGGAGG CCTGCAGAAG 
CTTCCTGTGG GGCAGGCTCT ACCCAAGGCC TATGGCACAG GTTTTGTGGG CTGTCTGCGG 
GACGTGGTAG TGGGCCATCG CCAGTTGCAT CTGCTGGAGG ACGCTGTCAC CAAACCAGAG 
CTAAGACCCT GCCCCACTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239603.1
CDS	91..6234
Translation	MVSLRLCSRAPLLPPLLLLVVAARTLPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYMWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFNGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	XM_006239541.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agrin (Agrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239541.3

ORF Insert Sequence:

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGAAA TCCCGCAAGG TTCCGCACAC CATGCTCAAC 
CTCAAGGAGC CTCTCTATAT TGGGGGAGCT CCTGACTTCA GCAAGCTGGC CAGGGGGGCT 
GCAGTGTCCT CTGGCTTCAA TGGTGTCATC CAGCTGGTGT CTCTAAGAGG CCATCAACTG 
CTGACTCAGG AGCATGTGTT GCGGGCAGTA GATGTCTCAC CTTTTGCAGA CCACCCTTGT 
ACCCAGGCCT TGGGCAACCC CTGCCTTAAT GGGGGCTCGT GTGTCCCGAG GGAAGCCACT 
TATGAATGCC TGTGTCCTGG GGGCTTCTCT GGGCTGCACT GCGAAAAGGG GCTAGTTGAG 
AAGTCAGTGG GGGACCTAGA AACACTGGCC TTTGATGGGC GGACCTACAT CGAATACCTC 
AATGCTGTGA TTGAGAGTGA GAAGGCACTG CAGAGCAACC ACTTTGAACT GAGCTTACGC 
ACTGAGGCCA CTCAGGGGCT GGTGCTGTGG ATTGGCAAGG CTGCAGAACG TGCAGATTAC 
ATGGCCCTGG CCATCGTGGA CGGGCACCTG CAACTGAGCT ATGACCTAGG TTCCCAGCCA 
GTTGTGCTGC GCTCCACTGT GAAGGTCAAC ACCAACCGCT GGCTTCGAAT CAGGGCTCAC 
AGGGAGCACA GGGAAGGTTC CCTTCAGGTG GGCAATGAAG CCCCTGTGAC TGGATCTTCC 
CCATTGGGTG CCACGCAACT GGACACAGAT GGAGCCCTGT GGCTTGGAGG CCTGCAGAAG 
CTTCCTGTGG GGCAGGCTCT ACCCAAGGCC TATGGCACAG GTTTTGTGGG CTGTCTGCGG 
GACGTGGTAG TGGGCCATCG CCAGTTGCAT CTGCTGGAGG ACGCTGTCAC CAAACCAGAG 
CTAAGACCCT GCCCCACTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_006239542.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239604.1 Rattus norvegicus agrin (Agrn), transcript variant X6, mRNA.

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CloneID	ORa28988
Clone ID Related Accession (Same CDS sequence)	XM_006239542.3
Accession Version	XM_006239542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239542.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGGTT CCGCACACCA TGCTCAACCT CAAGGAGCCT 
CTCTATATTG GGGGAGCTCC TGACTTCAGC AAGCTGGCCA GGGGGGCTGC AGTGTCCTCT 
GGCTTCAATG GTGTCATCCA GCTGGTGTCT CTAAGAGGCC ATCAACTGCT GACTCAGGAG 
CATGTGTTGC GGGCAGTAGA TGTCTCACCT TTTGCAGACC ACCCTTGTAC CCAGGCCTTG 
GGCAACCCCT GCCTTAATGG GGGCTCGTGT GTCCCGAGGG AAGCCACTTA TGAATGCCTG 
TGTCCTGGGG GCTTCTCTGG GCTGCACTGC GAAAAGGGGC TAGTTGAGAA GTCAGTGGGG 
GACCTAGAAA CACTGGCCTT TGATGGGCGG ACCTACATCG AATACCTCAA TGCTGTGATT 
GAGAGTGAGA AGGCACTGCA GAGCAACCAC TTTGAACTGA GCTTACGCAC TGAGGCCACT 
CAGGGGCTGG TGCTGTGGAT TGGCAAGGCT GCAGAACGTG CAGATTACAT GGCCCTGGCC 
ATCGTGGACG GGCACCTGCA ACTGAGCTAT GACCTAGGTT CCCAGCCAGT TGTGCTGCGC 
TCCACTGTGA AGGTCAACAC CAACCGCTGG CTTCGAATCA GGGCTCACAG GGAGCACAGG 
GAAGGTTCCC TTCAGGTGGG CAATGAAGCC CCTGTGACTG GATCTTCCCC ATTGGGTGCC 
ACGCAACTGG ACACAGATGG AGCCCTGTGG CTTGGAGGCC TGCAGAAGCT TCCTGTGGGG 
CAGGCTCTAC CCAAGGCCTA TGGCACAGGT TTTGTGGGCT GTCTGCGGGA CGTGGTAGTG 
GGCCATCGCC AGTTGCATCT GCTGGAGGAC GCTGTCACCA AACCAGAGCT AAGACCCTGC 
CCCACTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239604.1
CDS	91..6222
Translation	MVSLRLCSRAPLLPPLLLLVVAARTLPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYMWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFNGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	XM_006239542.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agrin (Agrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239542.3

ORF Insert Sequence:

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGGTT CCGCACACCA TGCTCAACCT CAAGGAGCCT 
CTCTATATTG GGGGAGCTCC TGACTTCAGC AAGCTGGCCA GGGGGGCTGC AGTGTCCTCT 
GGCTTCAATG GTGTCATCCA GCTGGTGTCT CTAAGAGGCC ATCAACTGCT GACTCAGGAG 
CATGTGTTGC GGGCAGTAGA TGTCTCACCT TTTGCAGACC ACCCTTGTAC CCAGGCCTTG 
GGCAACCCCT GCCTTAATGG GGGCTCGTGT GTCCCGAGGG AAGCCACTTA TGAATGCCTG 
TGTCCTGGGG GCTTCTCTGG GCTGCACTGC GAAAAGGGGC TAGTTGAGAA GTCAGTGGGG 
GACCTAGAAA CACTGGCCTT TGATGGGCGG ACCTACATCG AATACCTCAA TGCTGTGATT 
GAGAGTGAGA AGGCACTGCA GAGCAACCAC TTTGAACTGA GCTTACGCAC TGAGGCCACT 
CAGGGGCTGG TGCTGTGGAT TGGCAAGGCT GCAGAACGTG CAGATTACAT GGCCCTGGCC 
ATCGTGGACG GGCACCTGCA ACTGAGCTAT GACCTAGGTT CCCAGCCAGT TGTGCTGCGC 
TCCACTGTGA AGGTCAACAC CAACCGCTGG CTTCGAATCA GGGCTCACAG GGAGCACAGG 
GAAGGTTCCC TTCAGGTGGG CAATGAAGCC CCTGTGACTG GATCTTCCCC ATTGGGTGCC 
ACGCAACTGG ACACAGATGG AGCCCTGTGG CTTGGAGGCC TGCAGAAGCT TCCTGTGGGG 
CAGGCTCTAC CCAAGGCCTA TGGCACAGGT TTTGTGGGCT GTCTGCGGGA CGTGGTAGTG 
GGCCATCGCC AGTTGCATCT GCTGGAGGAC GCTGTCACCA AACCAGAGCT AAGACCCTGC 
CCCACTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_017593166.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448655.1 Rattus norvegicus agrin (Agrn), transcript variant X1, mRNA.

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CloneID	ORa61108
Clone ID Related Accession (Same CDS sequence)	XM_017593166.1
Accession Version	XM_017593166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGAAA TCCCGCAAGG TTCCGCACAC CATGCTCAAC 
CTCAAGGAGC CTCTCTATAT TGGGGGAGCT CCTGACTTCA GCAAGCTGGC CAGGGGGGCT 
GCAGTGTCCT CTGGCTTCAA TGGTGTCATC CAGCTGGTGT CTCTAAGAGG CCATCAACTG 
CTGACTCAGG AGCATGTGTT GCGGGCAGTA GATGTCTCAC CTTTTGCAGA CCACCCTTGT 
ACCCAGGCCT TGGGCAACCC CTGCCTTAAT GGGGGCTCGT GTGTCCCGAG GGAAGCCACT 
TATGAATGCC TGTGTCCTGG GGGCTTCTCT GGGCTGCACT GCGAAAAGGG GCTAGTTGAG 
AAGTCAGTGG GGGACCTAGA AACACTGGCC TTTGATGGGC GGACCTACAT CGAATACCTC 
AATGCTGTGA TTGAGAGCGA GCTGACCAAT GAGATCCCAG CCCCTGAAAC TCTGGATTCC 
CGGGCCCTTT TCAGTGAGAA GGCACTGCAG AGCAACCACT TTGAACTGAG CTTACGCACT 
GAGGCCACTC AGGGGCTGGT GCTGTGGATT GGCAAGGCTG CAGAACGTGC AGATTACATG 
GCCCTGGCCA TCGTGGACGG GCACCTGCAA CTGAGCTATG ACCTAGGTTC CCAGCCAGTT 
GTGCTGCGCT CCACTGTGAA GGTCAACACC AACCGCTGGC TTCGAATCAG GGCTCACAGG 
GAGCACAGGG AAGGTTCCCT TCAGGTGGGC AATGAAGCCC CTGTGACTGG ATCTTCCCCA 
TTGGGTGCCA CGCAACTGGA CACAGATGGA GCCCTGTGGC TTGGAGGCCT GCAGAAGCTT 
CCTGTGGGGC AGGCTCTACC CAAGGCCTAT GGCACAGGTT TTGTGGGCTG TCTGCGGGAC 
GTGGTAGTGG GCCATCGCCA GTTGCATCTG CTGGAGGACG CTGTCACCAA ACCAGAGCTA 
AGACCCTGCC CCACTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448655.1
CDS	91..6291
Translation	MVSLRLCSRAPLLPPLLLLVVAARTLPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYMWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFNGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	XM_017593166.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agrin (Agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593166.1

ORF Insert Sequence:

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGAAA TCCCGCAAGG TTCCGCACAC CATGCTCAAC 
CTCAAGGAGC CTCTCTATAT TGGGGGAGCT CCTGACTTCA GCAAGCTGGC CAGGGGGGCT 
GCAGTGTCCT CTGGCTTCAA TGGTGTCATC CAGCTGGTGT CTCTAAGAGG CCATCAACTG 
CTGACTCAGG AGCATGTGTT GCGGGCAGTA GATGTCTCAC CTTTTGCAGA CCACCCTTGT 
ACCCAGGCCT TGGGCAACCC CTGCCTTAAT GGGGGCTCGT GTGTCCCGAG GGAAGCCACT 
TATGAATGCC TGTGTCCTGG GGGCTTCTCT GGGCTGCACT GCGAAAAGGG GCTAGTTGAG 
AAGTCAGTGG GGGACCTAGA AACACTGGCC TTTGATGGGC GGACCTACAT CGAATACCTC 
AATGCTGTGA TTGAGAGCGA GCTGACCAAT GAGATCCCAG CCCCTGAAAC TCTGGATTCC 
CGGGCCCTTT TCAGTGAGAA GGCACTGCAG AGCAACCACT TTGAACTGAG CTTACGCACT 
GAGGCCACTC AGGGGCTGGT GCTGTGGATT GGCAAGGCTG CAGAACGTGC AGATTACATG 
GCCCTGGCCA TCGTGGACGG GCACCTGCAA CTGAGCTATG ACCTAGGTTC CCAGCCAGTT 
GTGCTGCGCT CCACTGTGAA GGTCAACACC AACCGCTGGC TTCGAATCAG GGCTCACAGG 
GAGCACAGGG AAGGTTCCCT TCAGGTGGGC AATGAAGCCC CTGTGACTGG ATCTTCCCCA 
TTGGGTGCCA CGCAACTGGA CACAGATGGA GCCCTGTGGC TTGGAGGCCT GCAGAAGCTT 
CCTGTGGGGC AGGCTCTACC CAAGGCCTAT GGCACAGGTT TTGTGGGCTG TCTGCGGGAC 
GTGGTAGTGG GCCATCGCCA GTTGCATCTG CTGGAGGACG CTGTCACCAA ACCAGAGCTA 
AGACCCTGCC CCACTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_017593167.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448656.1 Rattus norvegicus agrin (Agrn), transcript variant X2, mRNA.

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CloneID	ORa61109
Clone ID Related Accession (Same CDS sequence)	XM_017593167.1
Accession Version	XM_017593167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGGTT CCGCACACCA TGCTCAACCT CAAGGAGCCT 
CTCTATATTG GGGGAGCTCC TGACTTCAGC AAGCTGGCCA GGGGGGCTGC AGTGTCCTCT 
GGCTTCAATG GTGTCATCCA GCTGGTGTCT CTAAGAGGCC ATCAACTGCT GACTCAGGAG 
CATGTGTTGC GGGCAGTAGA TGTCTCACCT TTTGCAGACC ACCCTTGTAC CCAGGCCTTG 
GGCAACCCCT GCCTTAATGG GGGCTCGTGT GTCCCGAGGG AAGCCACTTA TGAATGCCTG 
TGTCCTGGGG GCTTCTCTGG GCTGCACTGC GAAAAGGGGC TAGTTGAGAA GTCAGTGGGG 
GACCTAGAAA CACTGGCCTT TGATGGGCGG ACCTACATCG AATACCTCAA TGCTGTGATT 
GAGAGCGAGC TGACCAATGA GATCCCAGCC CCTGAAACTC TGGATTCCCG GGCCCTTTTC 
AGTGAGAAGG CACTGCAGAG CAACCACTTT GAACTGAGCT TACGCACTGA GGCCACTCAG 
GGGCTGGTGC TGTGGATTGG CAAGGCTGCA GAACGTGCAG ATTACATGGC CCTGGCCATC 
GTGGACGGGC ACCTGCAACT GAGCTATGAC CTAGGTTCCC AGCCAGTTGT GCTGCGCTCC 
ACTGTGAAGG TCAACACCAA CCGCTGGCTT CGAATCAGGG CTCACAGGGA GCACAGGGAA 
GGTTCCCTTC AGGTGGGCAA TGAAGCCCCT GTGACTGGAT CTTCCCCATT GGGTGCCACG 
CAACTGGACA CAGATGGAGC CCTGTGGCTT GGAGGCCTGC AGAAGCTTCC TGTGGGGCAG 
GCTCTACCCA AGGCCTATGG CACAGGTTTT GTGGGCTGTC TGCGGGACGT GGTAGTGGGC 
CATCGCCAGT TGCATCTGCT GGAGGACGCT GTCACCAAAC CAGAGCTAAG ACCCTGCCCC 
ACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448656.1
CDS	91..6279
Translation	MVSLRLCSRAPLLPPLLLLVVAARTLPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYMWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFNGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	XM_017593167.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agrin (Agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593167.1

ORF Insert Sequence:

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGGTT CCGCACACCA TGCTCAACCT CAAGGAGCCT 
CTCTATATTG GGGGAGCTCC TGACTTCAGC AAGCTGGCCA GGGGGGCTGC AGTGTCCTCT 
GGCTTCAATG GTGTCATCCA GCTGGTGTCT CTAAGAGGCC ATCAACTGCT GACTCAGGAG 
CATGTGTTGC GGGCAGTAGA TGTCTCACCT TTTGCAGACC ACCCTTGTAC CCAGGCCTTG 
GGCAACCCCT GCCTTAATGG GGGCTCGTGT GTCCCGAGGG AAGCCACTTA TGAATGCCTG 
TGTCCTGGGG GCTTCTCTGG GCTGCACTGC GAAAAGGGGC TAGTTGAGAA GTCAGTGGGG 
GACCTAGAAA CACTGGCCTT TGATGGGCGG ACCTACATCG AATACCTCAA TGCTGTGATT 
GAGAGCGAGC TGACCAATGA GATCCCAGCC CCTGAAACTC TGGATTCCCG GGCCCTTTTC 
AGTGAGAAGG CACTGCAGAG CAACCACTTT GAACTGAGCT TACGCACTGA GGCCACTCAG 
GGGCTGGTGC TGTGGATTGG CAAGGCTGCA GAACGTGCAG ATTACATGGC CCTGGCCATC 
GTGGACGGGC ACCTGCAACT GAGCTATGAC CTAGGTTCCC AGCCAGTTGT GCTGCGCTCC 
ACTGTGAAGG TCAACACCAA CCGCTGGCTT CGAATCAGGG CTCACAGGGA GCACAGGGAA 
GGTTCCCTTC AGGTGGGCAA TGAAGCCCCT GTGACTGGAT CTTCCCCATT GGGTGCCACG 
CAACTGGACA CAGATGGAGC CCTGTGGCTT GGAGGCCTGC AGAAGCTTCC TGTGGGGCAG 
GCTCTACCCA AGGCCTATGG CACAGGTTTT GTGGGCTGTC TGCGGGACGT GGTAGTGGGC 
CATCGCCAGT TGCATCTGCT GGAGGACGCT GTCACCAAAC CAGAGCTAAG ACCCTGCCCC 
ACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_017593168.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448657.1 Rattus norvegicus agrin (Agrn), transcript variant X3, mRNA.

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CloneID	ORa61110
Clone ID Related Accession (Same CDS sequence)	XM_017593168.1
Accession Version	XM_017593168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGAAA TCCCGCAAGG TTCCGCACAC CATGCTCAAC 
CTCAAGGAGC CTCTCTATAT TGGGGGAGCT CCTGACTTCA GCAAGCTGGC CAGGGGGGCT 
GCAGTGTCCT CTGGCTTCAA TGGTGTCATC CAGCTGGTGT CTCTAAGAGG CCATCAACTG 
CTGACTCAGG AGCATGTGTT GCGGGCAGTA GATGTCTCAC CTTTTGCAGA CCACCCTTGT 
ACCCAGGCCT TGGGCAACCC CTGCCTTAAT GGGGGCTCGT GTGTCCCGAG GGAAGCCACT 
TATGAATGCC TGTGTCCTGG GGGCTTCTCT GGGCTGCACT GCGAAAAGGG GCTAGTTGAG 
AAGTCAGTGG GGGACCTAGA AACACTGGCC TTTGATGGGC GGACCTACAT CGAATACCTC 
AATGCTGTGA TTGAGAGCGA GCTGACCAAT GAGATCCCAG CTGAGAAGGC ACTGCAGAGC 
AACCACTTTG AACTGAGCTT ACGCACTGAG GCCACTCAGG GGCTGGTGCT GTGGATTGGC 
AAGGCTGCAG AACGTGCAGA TTACATGGCC CTGGCCATCG TGGACGGGCA CCTGCAACTG 
AGCTATGACC TAGGTTCCCA GCCAGTTGTG CTGCGCTCCA CTGTGAAGGT CAACACCAAC 
CGCTGGCTTC GAATCAGGGC TCACAGGGAG CACAGGGAAG GTTCCCTTCA GGTGGGCAAT 
GAAGCCCCTG TGACTGGATC TTCCCCATTG GGTGCCACGC AACTGGACAC AGATGGAGCC 
CTGTGGCTTG GAGGCCTGCA GAAGCTTCCT GTGGGGCAGG CTCTACCCAA GGCCTATGGC 
ACAGGTTTTG TGGGCTGTCT GCGGGACGTG GTAGTGGGCC ATCGCCAGTT GCATCTGCTG 
GAGGACGCTG TCACCAAACC AGAGCTAAGA CCCTGCCCCA CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448657.1
CDS	91..6258
Translation	MVSLRLCSRAPLLPPLLLLVVAARTLPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYMWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFNGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	XM_017593168.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agrin (Agrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593168.1

ORF Insert Sequence:

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GACTGGTTTC CCACTTTCTT TACGGGAGCT 
GCAACAGGAA CTACAGCTGC TATGGCCACA GCCAGAGCCA CCACTGTGAG CCGACTGCCG 
GCCTCTTCCG TCACCCCACG AGTCTACCCC AGTCACACCA GCCGGCCTGT TGGCAGAACT 
ACAGCACCAC CAACCACTCG CCGGCCACCA ACCACTGCCA CCAACATGGA CCGGCCTCGG 
ACCCCAGGCC ATCAACAGCC CTCAAAGTCC TGTGATTCCC AACCTTGCCT CCATGGAGGT 
ACCTGCCAGG ACCAGGATTC TGGCAAGGGT TTCACCTGCA GCTGTACTGC AGGCAGGGGT 
GGCTCTGTCT GTGAGAAAGT ACAACCCCCC TCCATGCCAG CTTTTAAGGG CCACTCCTTC 
TTGGCCTTCC CCACCCTCCG AGCCTACCAC ACACTGCGCC TAGCACTGGA ATTCCGGGCT 
TTGGAGACAG AGGGACTGCT ACTCTACAAT GGCAATGCAC GTGGCAAAGA TTTCCTGGCT 
CTGGCTCTGT TGGATGGGCG TGTGCAGTTC AGGTTTGACA CAGGCTCAGG GCCAGCAGTG 
CTAACGAGCT TAGTGCCAGT GGAACCAGGA CGGTGGCACC GCCTCGAGTT GTCACGGCAT 
TGGCGTCAGG GCACACTTTC TGTGGATGGC GAGACTCCTG TTGTAGGTGA AAGTCCCAGT 
GGCACCGATG GCCTCAACTT GGACACGAAC CTCTATGTGG GTGGTATCCC AGAAGAACAA 
GTTGCCATGG TGCTTGATCG GACCTCTGTG GGCGTTGGCC TGAAAGGATG CATTCGTATG 
CTGGATATCA ACAATCAGCA GCTGGAGCTT AGCGACTGGC AGAGGGCTGC GGTTCAAAGC 
TCTGGTGTGG GAGAATGTGG AGACCATCCG TGCTTACCTA ACCCCTGCCA TGGTGGGGCC 
CTATGCCAGG CACTGGAGGC TGGCATGTTC CTCTGTCAGT GCCCGCCTGG CCGCTTTGGC 
CCAACTTGTG CAGATGAAAA GAGCCCCTGC CAGCCGAACC CCTGCCATGG GGCAGCCCCC 
TGTCGTGTGC TTTCCAGCGG TGGGGCCAAG TGCGAGTGCC CCCTGGGACG CAGTGGTACC 
TTCTGTCAGA CAGTCCTGGA GACTGCTGGC TCCCGGCCCT TCCTGGCTGA CTTTAATGGC 
TTCTCCTACC TGGAACTGAA AGGCTTGCAC ACCTTCGAGA GAGACCTAGG GGAGAAGATG 
GCGCTGGAGA TGGTGTTCTT GGCTCGTGGG CCCAGTGGCT TACTCCTCTA CAATGGGCAG 
AAGACAGATG GCAAGGGGGA CTTTGTATCC CTGGCCCTGC ATAACCGGCA CCTAGAGTTC 
TGCTATGACC TTGGCAAGGG GGCTGCAGTC ATCAGGAGCA AAGAGCCCAT AGCCCTGGGA 
ACCTGGGTCA GGGTATTCCT GGAACGAAAT GGCCGCAAGG GTGCCCTTCA AGTGGGTGAT 
GGTCCCCGTG TGCTAGGGGA ATCTCCGAAA TCCCGCAAGG TTCCGCACAC CATGCTCAAC 
CTCAAGGAGC CTCTCTATAT TGGGGGAGCT CCTGACTTCA GCAAGCTGGC CAGGGGGGCT 
GCAGTGTCCT CTGGCTTCAA TGGTGTCATC CAGCTGGTGT CTCTAAGAGG CCATCAACTG 
CTGACTCAGG AGCATGTGTT GCGGGCAGTA GATGTCTCAC CTTTTGCAGA CCACCCTTGT 
ACCCAGGCCT TGGGCAACCC CTGCCTTAAT GGGGGCTCGT GTGTCCCGAG GGAAGCCACT 
TATGAATGCC TGTGTCCTGG GGGCTTCTCT GGGCTGCACT GCGAAAAGGG GCTAGTTGAG 
AAGTCAGTGG GGGACCTAGA AACACTGGCC TTTGATGGGC GGACCTACAT CGAATACCTC 
AATGCTGTGA TTGAGAGCGA GCTGACCAAT GAGATCCCAG CTGAGAAGGC ACTGCAGAGC 
AACCACTTTG AACTGAGCTT ACGCACTGAG GCCACTCAGG GGCTGGTGCT GTGGATTGGC 
AAGGCTGCAG AACGTGCAGA TTACATGGCC CTGGCCATCG TGGACGGGCA CCTGCAACTG 
AGCTATGACC TAGGTTCCCA GCCAGTTGTG CTGCGCTCCA CTGTGAAGGT CAACACCAAC 
CGCTGGCTTC GAATCAGGGC TCACAGGGAG CACAGGGAAG GTTCCCTTCA GGTGGGCAAT 
GAAGCCCCTG TGACTGGATC TTCCCCATTG GGTGCCACGC AACTGGACAC AGATGGAGCC 
CTGTGGCTTG GAGGCCTGCA GAAGCTTCCT GTGGGGCAGG CTCTACCCAA GGCCTATGGC 
ACAGGTTTTG TGGGCTGTCT GCGGGACGTG GTAGTGGGCC ATCGCCAGTT GCATCTGCTG 
GAGGACGCTG TCACCAAACC AGAGCTAAGA CCCTGCCCCA CTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_017593169.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448658.1 Rattus norvegicus agrin (Agrn), transcript variant X4, mRNA.

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CloneID	ORa61111
Clone ID Related Accession (Same CDS sequence)	XM_017593169.1
Accession Version	XM_017593169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GGAACTACAG CTGCTATGGC CACAGCCAGA 
GCCACCACTG TGAGCCGACT GCCGGCCTCT TCCGTCACCC CACGAGTCTA CCCCAGTCAC 
ACCAGCCGGC CTGTTGGCAG AACTACAGCA CCACCAACCA CTCGCCGGCC ACCAACCACT 
GCCACCAACA TGGACCGGCC TCGGACCCCA GGCCATCAAC AGCCCTCAAA GTCCTGTGAT 
TCCCAACCTT GCCTCCATGG AGGTACCTGC CAGGACCAGG ATTCTGGCAA GGGTTTCACC 
TGCAGCTGTA CTGCAGGCAG GGGTGGCTCT GTCTGTGAGA AAGTACAACC CCCCTCCATG 
CCAGCTTTTA AGGGCCACTC CTTCTTGGCC TTCCCCACCC TCCGAGCCTA CCACACACTG 
CGCCTAGCAC TGGAATTCCG GGCTTTGGAG ACAGAGGGAC TGCTACTCTA CAATGGCAAT 
GCACGTGGCA AAGATTTCCT GGCTCTGGCT CTGTTGGATG GGCGTGTGCA GTTCAGGTTT 
GACACAGGCT CAGGGCCAGC AGTGCTAACG AGCTTAGTGC CAGTGGAACC AGGACGGTGG 
CACCGCCTCG AGTTGTCACG GCATTGGCGT CAGGGCACAC TTTCTGTGGA TGGCGAGACT 
CCTGTTGTAG GTGAAAGTCC CAGTGGCACC GATGGCCTCA ACTTGGACAC GAACCTCTAT 
GTGGGTGGTA TCCCAGAAGA ACAAGTTGCC ATGGTGCTTG ATCGGACCTC TGTGGGCGTT 
GGCCTGAAAG GATGCATTCG TATGCTGGAT ATCAACAATC AGCAGCTGGA GCTTAGCGAC 
TGGCAGAGGG CTGCGGTTCA AAGCTCTGGT GTGGGAGAAT GTGGAGACCA TCCGTGCTTA 
CCTAACCCCT GCCATGGTGG GGCCCTATGC CAGGCACTGG AGGCTGGCAT GTTCCTCTGT 
CAGTGCCCGC CTGGCCGCTT TGGCCCAACT TGTGCAGATG AAAAGAGCCC CTGCCAGCCG 
AACCCCTGCC ATGGGGCAGC CCCCTGTCGT GTGCTTTCCA GCGGTGGGGC CAAGTGCGAG 
TGCCCCCTGG GACGCAGTGG TACCTTCTGT CAGACAGTCC TGGAGACTGC TGGCTCCCGG 
CCCTTCCTGG CTGACTTTAA TGGCTTCTCC TACCTGGAAC TGAAAGGCTT GCACACCTTC 
GAGAGAGACC TAGGGGAGAA GATGGCGCTG GAGATGGTGT TCTTGGCTCG TGGGCCCAGT 
GGCTTACTCC TCTACAATGG GCAGAAGACA GATGGCAAGG GGGACTTTGT ATCCCTGGCC 
CTGCATAACC GGCACCTAGA GTTCTGCTAT GACCTTGGCA AGGGGGCTGC AGTCATCAGG 
AGCAAAGAGC CCATAGCCCT GGGAACCTGG GTCAGGGTAT TCCTGGAACG AAATGGCCGC 
AAGGGTGCCC TTCAAGTGGG TGATGGTCCC CGTGTGCTAG GGGAATCTCC GAAATCCCGC 
AAGGTTCCGC ACACCATGCT CAACCTCAAG GAGCCTCTCT ATATTGGGGG AGCTCCTGAC 
TTCAGCAAGC TGGCCAGGGG GGCTGCAGTG TCCTCTGGCT TCAATGGTGT CATCCAGCTG 
GTGTCTCTAA GAGGCCATCA ACTGCTGACT CAGGAGCATG TGTTGCGGGC AGTAGATGTC 
TCACCTTTTG CAGACCACCC TTGTACCCAG GCCTTGGGCA ACCCCTGCCT TAATGGGGGC 
TCGTGTGTCC CGAGGGAAGC CACTTATGAA TGCCTGTGTC CTGGGGGCTT CTCTGGGCTG 
CACTGCGAAA AGGGGCTAGT TGAGAAGTCA GTGGGGGACC TAGAAACACT GGCCTTTGAT 
GGGCGGACCT ACATCGAATA CCTCAATGCT GTGATTGAGA GCGAGCTGAC CAATGAGATC 
CCAGCCCCTG AAACTCTGGA TTCCCGGGCC CTTTTCAGTG AGAAGGCACT GCAGAGCAAC 
CACTTTGAAC TGAGCTTACG CACTGAGGCC ACTCAGGGGC TGGTGCTGTG GATTGGCAAG 
GCTGCAGAAC GTGCAGATTA CATGGCCCTG GCCATCGTGG ACGGGCACCT GCAACTGAGC 
TATGACCTAG GTTCCCAGCC AGTTGTGCTG CGCTCCACTG TGAAGGTCAA CACCAACCGC 
TGGCTTCGAA TCAGGGCTCA CAGGGAGCAC AGGGAAGGTT CCCTTCAGGT GGGCAATGAA 
GCCCCTGTGA CTGGATCTTC CCCATTGGGT GCCACGCAAC TGGACACAGA TGGAGCCCTG 
TGGCTTGGAG GCCTGCAGAA GCTTCCTGTG GGGCAGGCTC TACCCAAGGC CTATGGCACA 
GGTTTTGTGG GCTGTCTGCG GGACGTGGTA GTGGGCCATC GCCAGTTGCA TCTGCTGGAG 
GACGCTGTCA CCAAACCAGA GCTAAGACCC TGCCCCACTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448658.1
CDS	91..6255
Translation	MVSLRLCSRAPLLPPLLLLVVAARTLPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYMWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFNGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	XM_017593169.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus agrin (Agrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593169.1

ORF Insert Sequence:

ATGGTCAGCC TGCGGCTGTG TTCCCGGGCC CCGCTGCTGC CACCGCTGCT GCTCTTGGTG 
GTGGCCGCGC GAACCCTGCC GGGCGCCAGC GGGACGTGCC CGGAGCGCGC CCTGGAGCGG 
CGCGAGGAGG AGGCGAATGT GGTGCTCACC GGCACAGTGG AGGAGATCCT CAACGTGGAC 
CCGGTGCAGC ACACGTACTC ATGCAAGGTC CGAGTGTGGC GGTACTTGAA AGGCAAAGAT 
GTGGTGGCCC AGGAGAGTCT GCTGGATGGT GGCAACAAGG TGGTAATTGG TGGCTTTGGA 
GACCCCCTCA TCTGCGACAA CCAGGTGTCC ACTGGGGACA CTAGAATCTT TTTTGTGAAC 
CCGGCACCAC CCTACATGTG GCCAGCCCAC AAGAATGAGT TGATGCTTAA CTCCAGCCTT 
ATGCGGATCA CTTTGCGGAA CCTGGAAGAG GTGGAGTTCT GTGTAGAAGA CAAACCTGGA 
ATCCACTTCA CACCAGCGCC TCCTACGCCT CCTGATGTAT GCAGGGGAAT GTTATGTGGC 
TTTGGTGCTG TGTGTGAACC TAGTGTTGAG GATCCAGGCC GTGCCTCCTG TGTGTGCAAG 
AAGAATGCTT GCCCTGCTAC GGTGGCTCCT GTGTGTGGCT CAGATGCCTC CACCTATAGC 
AACGAGTGCG AGCTGCAGCG CGCGCAGTGC AACCAGCAAC GGCGCATCCG CCTGCTTCGC 
CAAGGGCCAT GTGGGTCCCG GGACCCCTGT GCCAACGTGA CCTGCAGTTT CGGTAGTACC 
TGTGTACCTT CGGCTGACGG ACAGACTGCC TCATGTCTGT GTCCTACAAC CTGCTTCGGG 
GCCCCTGATG GCACAGTGTG TGGCAGTGAC GGTGTTGACT ACCCTAGTGA ATGCCAGCTG 
CTTAGTCATG CCTGTGCCAG CCAGGAGCAC ATCTTCAAGA AGTTCAATGG TCCTTGTGAT 
CCCTGCCAGG GCAGCATGTC AGACCTGAAC CACATTTGCC GTGTGAACCC ACGTACACGG 
CACCCAGAAA TGCTTCTGCG GCCTGAGAAC TGCCCTGCCC AGCACACGCC TATCTGTGGA 
GATGATGGGG TCACCTATGA AAACGACTGT GTCATGAGCC GTATAGGTGC AACCCGTGGC 
CTGCTTCTCC AGAAAGTACG CTCTGGTCAA TGCCAAACTC GAGACCAGTG CCCGGAGACC 
TGCCAGTTTA ACTCCGTATG CCTGTCCCGC CGTGGCCGTC CCCACTGTTC CTGCGATCGT 
GTCACCTGTG ATGGGTCTTA CAGGCCTGTG TGTGCCCAAG ATGGGCACAC ATACAACAAT 
GACTGTTGGC GCCAACAGGC TGAGTGTCGA CAACAGCGGG CCATTCCTCC CAAGCACCAG 
GGCCCGTGTG ACCAGACCCC GTCCCCATGC CATGGAGTGC AATGTGCATT TGGGGCAGTA 
TGCACAGTGA AGAACGGGAA AGCCGAGTGT GAGTGCCAGC GGGTGTGCTC CGGCATCTAC 
GATCCTGTGT GCGGCAGTGA CGGTGTCACT TACGGCAGTG TGTGCGAGCT GGAATCCATG 
GCCTGTACCC TTGGGCGGGA AATCCAAGTG GCCCGCAGAG GACCCTGTGA CCCATGTGGG 
CAGTGCCGTT TTGGATCCCT GTGCGAGGTG GAAACTGGAC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGAGT CAGCCCAGCC CGTATGCGGT TCTGATGGAC ACACATACGC TAGTGAATGT 
GAGCTGCATG TCCACGCCTG TACGCACCAG ATCAGCCTAT ACGTGGCCTC AGCTGGACAC 
TGCCAGACCT GTGGAGAAAA AGTTTGTACT TTTGGGGCTG TGTGCTCAGC TGGACAGTGT 
GTATGTCCCC GTTGTGAGCA CCCTCCACCT GGCCCTGTGT GTGGCAGTGA TGGTGTCACC 
TACCTCAGTG CCTGTGAGCT ACGCGAAGCT GCCTGTCAGC AGCAGGTACA AATTGAGGAG 
GCCCATGCAG GGCCATGTGA GCCGGCTGAG TGTGGCTCAG GGGGCTCTGG TTCTGGGGAG 
GACGACGAGT GTGAACAGGA GCTATGCCGG CAGCGTGGTG GTATCTGGGA CGAGGACTCA 
GAAGATGGGC CATGTGTCTG TGACTTTAGC TGCCAGAGTG TCCCTAGAAG CCCAGTGTGT 
GGCTCGGATG GAGTCACCTA TGGCACCGAG TGTGATCTGA AGAAGGCCAG GTGCGAATCA 
CAGCAAGAAC TGTACGTTGC TGCTCAGGGA GCCTGCCGTG GCCCTACCTT GGCTCCACTG 
CTACCTGTGG CCTTCCCACA CTGTGCCCAA ACCCCCTATG GCTGCTGCCA GGACAATTTC 
ACTGCTGCCC AGGGTGTGGG CTTGGCTGGC TGTCCCAGCA CCTGCCATTG CAACCCCCAT 
GGCTCCTACA GTGGCACTTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGTGTA 
GGAGGCCTCA GGTGTGATCG CTGTGAACCT GGCTTCTGGA ACTTCCGTGG CATCGTCACC 
GATGGACATA GTGGTTGCAC TCCCTGCAGC TGCGACCCTC GGGGTGCCGT AAGGGATGAC 
TGTGAACAGA TGACTGGATT GTGTTCCTGT AGGCCTGGTG TGGCTGGTCC CAAGTGTGGA 
CAGTGTCCAG ATGGTCAAGT CCTGGGCCAT CTAGGCTGTG AAGCAGATCC CATGACACCT 
GTGACTTGTG TGGAGATACA CTGCGAGTTC GGGGCCTCCT GCGTAGAGAA GGCTGGTTTT 
GCCCAATGTA TCTGCCCAAC CCTCACATGT CCAGAGGCTA ACTCTACCAA GGTCTGCGGA 
TCAGACGGTG TGACATATGG CAATGAATGC CAGCTGAAGG CCATTGCCTG CCGCCAGCGT 
CTGGACATCT CCACTCAGAG TCTTGGTCCC TGCCAGGAGA GTGTTACTCC TGGGGCTTCC 
CCAACATCTG CATCTATGAC TACCCCAAGG CATATCCTGA GCAAGACGCT GCCATTTCCC 
CACAACAGCC TTCCTCTGTC TCCCGGCAGT ACTACCCATG ATTGGCCCAC CCCATTACCC 
ATATCACCTC ACACCACAGT CAGCATCCCC AGAAGCACCG CGTGGCCTGT GCTGACCGTG 
CCCCCTACAG CAGCAGCCTC TGACGTAACC AGTCTCGCAA CATCAATCTT CAGTGAATCC 
GGCAGCGCCA ATGGGAGTGG CGACGAGGAA CTGAGTGGAG ATGAGGAGGC CAGTGGGGGC 
GGGTCTGGGG GACTTGAGCC CCCGGTGGGC AGCATTGTGG TGACCCATGG GCCACCCATC 
GAGAGGGCTT CCTGCTACAA CTCACCTTTG GGCTGCTGCT CAGATGGCAA GACACCCTCA 
CTGGACTCCG AAGGCTCCAA CTGTCCTGCT ACCAAGGCAT TCCAGGGCGT GCTGGAGCTT 
GAGGGGGTCG AGGGACAGGA ACTGTTCTAC ACACCTGAGA TGGCTGACCC CAAGTCAGAG 
TTGTTTGGGG AGACCGCAAG GAGCATTGAA AGCACGCTGG ACGACCTGTT CCGGAATTCA 
GACGTCAAGA AGGACTTCTG GAGTGTCCGC CTACGGGAAC TGGGGCCTGG CAAGTTGGTC 
CGCGCCATTG TGGACGTTCA CTTTGACCCT ACCACAGCCT TCCAGGCATC AGATGTGGGT 
CAGGCCTTGC TCCGACAGAT CCAGGTATCT AGGCCATGGG CCCTGGCAGT GAGGAGGCCT 
CTGCAGGAAC ATGTGCGATT CTTAGACTTT GGAACTACAG CTGCTATGGC CACAGCCAGA 
GCCACCACTG TGAGCCGACT GCCGGCCTCT TCCGTCACCC CACGAGTCTA CCCCAGTCAC 
ACCAGCCGGC CTGTTGGCAG AACTACAGCA CCACCAACCA CTCGCCGGCC ACCAACCACT 
GCCACCAACA TGGACCGGCC TCGGACCCCA GGCCATCAAC AGCCCTCAAA GTCCTGTGAT 
TCCCAACCTT GCCTCCATGG AGGTACCTGC CAGGACCAGG ATTCTGGCAA GGGTTTCACC 
TGCAGCTGTA CTGCAGGCAG GGGTGGCTCT GTCTGTGAGA AAGTACAACC CCCCTCCATG 
CCAGCTTTTA AGGGCCACTC CTTCTTGGCC TTCCCCACCC TCCGAGCCTA CCACACACTG 
CGCCTAGCAC TGGAATTCCG GGCTTTGGAG ACAGAGGGAC TGCTACTCTA CAATGGCAAT 
GCACGTGGCA AAGATTTCCT GGCTCTGGCT CTGTTGGATG GGCGTGTGCA GTTCAGGTTT 
GACACAGGCT CAGGGCCAGC AGTGCTAACG AGCTTAGTGC CAGTGGAACC AGGACGGTGG 
CACCGCCTCG AGTTGTCACG GCATTGGCGT CAGGGCACAC TTTCTGTGGA TGGCGAGACT 
CCTGTTGTAG GTGAAAGTCC CAGTGGCACC GATGGCCTCA ACTTGGACAC GAACCTCTAT 
GTGGGTGGTA TCCCAGAAGA ACAAGTTGCC ATGGTGCTTG ATCGGACCTC TGTGGGCGTT 
GGCCTGAAAG GATGCATTCG TATGCTGGAT ATCAACAATC AGCAGCTGGA GCTTAGCGAC 
TGGCAGAGGG CTGCGGTTCA AAGCTCTGGT GTGGGAGAAT GTGGAGACCA TCCGTGCTTA 
CCTAACCCCT GCCATGGTGG GGCCCTATGC CAGGCACTGG AGGCTGGCAT GTTCCTCTGT 
CAGTGCCCGC CTGGCCGCTT TGGCCCAACT TGTGCAGATG AAAAGAGCCC CTGCCAGCCG 
AACCCCTGCC ATGGGGCAGC CCCCTGTCGT GTGCTTTCCA GCGGTGGGGC CAAGTGCGAG 
TGCCCCCTGG GACGCAGTGG TACCTTCTGT CAGACAGTCC TGGAGACTGC TGGCTCCCGG 
CCCTTCCTGG CTGACTTTAA TGGCTTCTCC TACCTGGAAC TGAAAGGCTT GCACACCTTC 
GAGAGAGACC TAGGGGAGAA GATGGCGCTG GAGATGGTGT TCTTGGCTCG TGGGCCCAGT 
GGCTTACTCC TCTACAATGG GCAGAAGACA GATGGCAAGG GGGACTTTGT ATCCCTGGCC 
CTGCATAACC GGCACCTAGA GTTCTGCTAT GACCTTGGCA AGGGGGCTGC AGTCATCAGG 
AGCAAAGAGC CCATAGCCCT GGGAACCTGG GTCAGGGTAT TCCTGGAACG AAATGGCCGC 
AAGGGTGCCC TTCAAGTGGG TGATGGTCCC CGTGTGCTAG GGGAATCTCC GAAATCCCGC 
AAGGTTCCGC ACACCATGCT CAACCTCAAG GAGCCTCTCT ATATTGGGGG AGCTCCTGAC 
TTCAGCAAGC TGGCCAGGGG GGCTGCAGTG TCCTCTGGCT TCAATGGTGT CATCCAGCTG 
GTGTCTCTAA GAGGCCATCA ACTGCTGACT CAGGAGCATG TGTTGCGGGC AGTAGATGTC 
TCACCTTTTG CAGACCACCC TTGTACCCAG GCCTTGGGCA ACCCCTGCCT TAATGGGGGC 
TCGTGTGTCC CGAGGGAAGC CACTTATGAA TGCCTGTGTC CTGGGGGCTT CTCTGGGCTG 
CACTGCGAAA AGGGGCTAGT TGAGAAGTCA GTGGGGGACC TAGAAACACT GGCCTTTGAT 
GGGCGGACCT ACATCGAATA CCTCAATGCT GTGATTGAGA GCGAGCTGAC CAATGAGATC 
CCAGCCCCTG AAACTCTGGA TTCCCGGGCC CTTTTCAGTG AGAAGGCACT GCAGAGCAAC 
CACTTTGAAC TGAGCTTACG CACTGAGGCC ACTCAGGGGC TGGTGCTGTG GATTGGCAAG 
GCTGCAGAAC GTGCAGATTA CATGGCCCTG GCCATCGTGG ACGGGCACCT GCAACTGAGC 
TATGACCTAG GTTCCCAGCC AGTTGTGCTG CGCTCCACTG TGAAGGTCAA CACCAACCGC 
TGGCTTCGAA TCAGGGCTCA CAGGGAGCAC AGGGAAGGTT CCCTTCAGGT GGGCAATGAA 
GCCCCTGTGA CTGGATCTTC CCCATTGGGT GCCACGCAAC TGGACACAGA TGGAGCCCTG 
TGGCTTGGAG GCCTGCAGAA GCTTCCTGTG GGGCAGGCTC TACCCAAGGC CTATGGCACA 
GGTTTTGTGG GCTGTCTGCG GGACGTGGTA GTGGGCCATC GCCAGTTGCA TCTGCTGGAG 
GACGCTGTCA CCAAACCAGA GCTAAGACCC TGCCCCACTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Agrin requires specific proteins to selectively activate γ-aminobutyric acid neurons for pain suppression.
Experimental neurology261646-53(2014 Nov)
Erasso D,Tender G,Levitt RC,Cui JG

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
The Journal of biological chemistry288(26)18716-31(2013 Jun)
Soteriou D,Iskender B,Byron A,Humphries JD,Borg-Bartolo S,Haddock MC,Baxter MA,Knight D,Humphries MJ,Kimber SJ

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Agrn cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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