Hspe1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspe1
Entrez Gene ID	25462
Full Name	heat shock protein family E member 1
Synonyms	Cpn10
General protein information	Preferred Names heat shock protein family E member 1 Names 10 kDa heat shock protein, mitochondrial 10 kDa chaperonin Heat shock 10 kD protein 1 (chaperonin 10) chaperonin 10 heat shock 10 kDa protein 1 (chaperonin 10) heat shock protein 1 (chaperonin 10) hsp10
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q31
Summary	stress-induced mitochondrial matrix protein and molecular chaperone [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012966.1	NP_037098.1	10 kDa heat shock protein, mitochondrial

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC721815	XP_001118014.2
Rattus norvegicus (Norway rat)	Hspe1	NP_037098.1
Xenopus tropicalis (tropical clawed frog)	hspe1	NP_001006882.1
Kluyveromyces lactis	KLLA0E09307g	XP_454370.1
Oryza sativa (rice)	Os07g0641700	NP_001060431.1
Schizosaccharomyces pombe (fission yeast)	hsp10	NP_588098.1
Neurospora crassa	NCU04334	XP_956315.1
Macaca mulatta (Rhesus monkey)	LOC696316	XP_001084953.1
Macaca mulatta (Rhesus monkey)	LOC720015	XP_001114974.1
Canis lupus familiaris (dog)	HSPE1	XP_536017.2
Danio rerio (zebrafish)	hspe1	NP_571601.1
Bos taurus (cattle)	HSPE1	NP_776771.1
Mus musculus (house mouse)	Hspe1	NP_032329.1
Arabidopsis thaliana (thale cress)	CPN10	NP_563961.1
Arabidopsis thaliana (thale cress)	AT1G23100	NP_173723.1
Macaca mulatta (Rhesus monkey)	HSPE1	XP_002799059.1
Magnaporthe oryzae (rice blast fungus)	MGG_13221	XP_003711926.1
Canis lupus familiaris (dog)	LOC609748	XP_852207.1
Caenorhabditis elegans (roundworm)	Y22D7AL.10	NP_497428.1
Oryza sativa (rice)	Os03g0366000	NP_001050177.2
Eremothecium gossypii	AGOS_AEL235W	NP_984626.2
Homo sapiens (human)	HSPE1	NP_002148.1
Macaca mulatta (Rhesus monkey)	LOC711695	XP_001100531.2
Macaca mulatta (Rhesus monkey)	LOC710026	XP_001105898.1
Saccharomyces cerevisiae (baker's yeast)	HSP10	NP_014663.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Fibril formation of hsp10 homologue proteins and determination of fibril core regions: differences in fibril core regions dependent on subtle differences in amino acid sequence.
Yagi H, Sato A, Yoshida A, Hattori Y, Hara M, Shimamura J, Sakane I, Hongo K, Mizobata T, Kawata Y
Journal of molecular biology377(5)1593-606(2008 Apr)

Differential expression profiling of membrane proteins by quantitative proteomics in a human mesenchymal stem cell line undergoing osteoblast differentiation.
Foster LJ, Zeemann PA, Li C, Mann M, Jensen ON, Kassem M
Stem cells (Dayton, Ohio)23(9)1367-77(2005 Oct)

Structural stability of oligomeric chaperonin 10: the role of two beta-strands at the N and C termini in structural stabilization.
Sakane I, Ikeda M, Matsumoto C, Higurashi T, Inoue K, Hongo K, Mizobata T, Kawata Y
Journal of molecular biology344(4)1123-33(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hspe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12431	NM_012966.1 Latest version!	Rattus norvegicus heat shock protein family E (Hsp10) member 1 (Hspe1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspe1 ( NM_012966.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037098.1 Rattus norvegicus heat shock protein family E (Hsp10) member 1 (Hspe1), mRNA.

Price & Availability↑

CloneID	ORa12431
Clone ID Related Accession (Same CDS sequence)	NM_012966.1
Accession Version	NM_012966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	10 kDa heat shock protein, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U68562.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ220992.1, CF977228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTGGAC AAGCTTTTAG GAAGTTTCTT CCGCTATTTG ACAGAGTATT GGTTGAAAGG 
AGTGCCGCTG AAACTGTGAC CAAAGGTGGC ATTATGCTTC CAGAAAAGTC TCAAGGAAAA 
GTATTGCAAG CAACGGTCGT GGCTGTGGGA TCAGGCGGGA AAGGAAAGGG TGGAGAGATT 
CAGCCTGTCA GTGTGAAAGT TGGAGATAAA GTTCTTCTCC CAGAACACGG AGGCACCAAA 
GTAGTACTAG ACGACAAGGA TTACTTCTTA TTTAGAGATG GCGACATTCT TGGAAAGTAT 
GTCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037098.1
CDS	272..580
Translation	MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGGKGKGGEIQPVSVKVGDKVLLPEHGGTKVVLDDKDYFLFRDGDILGKYVD

Target ORF information:

RefSeq Version	NM_012966.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein family E (Hsp10) member 1 (Hspe1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012966.1

ORF Insert Sequence:

ATGGCTGGAC AAGCTTTTAG GAAGTTTCTT CCGCTATTTG ACAGAGTATT GGTTGAAAGG 
AGTGCCGCTG AAACTGTGAC CAAAGGTGGC ATTATGCTTC CAGAAAAGTC TCAAGGAAAA 
GTATTGCAAG CAACGGTCGT GGCTGTGGGA TCAGGCGGGA AAGGAAAGGG TGGAGAGATT 
CAGCCTGTCA GTGTGAAAGT TGGAGATAAA GTTCTTCTCC CAGAACACGG AGGCACCAAA 
GTAGTACTAG ACGACAAGGA TTACTTCTTA TTTAGAGATG GCGACATTCT TGGAAAGTAT 
GTCGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Fibril formation of hsp10 homologue proteins and determination of fibril core regions: differences in fibril core regions dependent on subtle differences in amino acid sequence.
Journal of molecular biology377(5)1593-606(2008 Apr)
Yagi H,Sato A,Yoshida A,Hattori Y,Hara M,Shimamura J,Sakane I,Hongo K,Mizobata T,Kawata Y

Differential expression profiling of membrane proteins by quantitative proteomics in a human mesenchymal stem cell line undergoing osteoblast differentiation.
Stem cells (Dayton, Ohio)23(9)1367-77(2005 Oct)
Foster LJ,Zeemann PA,Li C,Mann M,Jensen ON,Kassem M

Structural stability of oligomeric chaperonin 10: the role of two beta-strands at the N and C termini in structural stabilization.
Journal of molecular biology344(4)1123-33(2004 Dec)
Sakane I,Ikeda M,Matsumoto C,Higurashi T,Inoue K,Hongo K,Mizobata T,Kawata Y