Cyp2e1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp2e1
Entrez Gene ID	25086
Full Name	cytochrome P450, family 2, subfamily e, polypeptide 1
Synonyms	Cyp2e
General protein information	Preferred Names cytochrome P450, family 2, subfamily e, polypeptide 1 Names cytochrome P450 2E1 4-nitrophenol 2-hydroxylase CYPIIE1 P450-J P450RLM6 cytochrome P450 subfamily 2e1 (ethanol-inducible) cytochrome P450, subfamily 2E, polypeptide 1 cytochrome P450-J cytochrome P450RLM6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41
Summary	facilitates hepatocyte sensitization to tumor necrosis factor-alpha (TNF-alpha)-mediated cell death; may play a role in xenobiotic metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031543.1	NP_113731.1	cytochrome P450 2E1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CYP2E1	NP_000764.1
Pan troglodytes (chimpanzee)	LOC450857	XP_508139.3
Macaca mulatta (Rhesus monkey)	CYP2E1	NP_001035303.1
Bos taurus (cattle)	CYP2E1	NP_776955.2
Mus musculus (house mouse)	Cyp2e1	NP_067257.1
Canis lupus familiaris (dog)	CYP2E1	NP_001003339.1
Rattus norvegicus (Norway rat)	Cyp2e1	NP_113731.1

Gene Ontology
Bibliography

Related articles in PubMed

High CYP2E1 activity aggravates hepatofibrosis by limiting macrophage polarization towards the M2 phenotype.
Guo YY, Xu C, Fang Y, Wang CE, Gao N, Wen Q, Qiao HL
Molecular carcinogenesis58(8)1481-1491(2019 08)

Red meat intake, CYP2E1 and PPARγ polymorphisms, and colorectal cancer risk.
Kim NH, Seol JE, Kim J, Lee BH, Hwang DY, Jeong J, Lee HJ, Ahn YO, Kim DH, Lee JE
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)28(4)304-310(2019 Jul)

Chemopreventive efficacy zingerone (4-[4-hydroxy-3-methylphenyl] butan-2-one) in experimental colon carcinogenesis in Wistar rats.
Ganaie MA, Al Saeedan A, Madhkali H, Jan BL, Khatlani T, Sheikh IA, Rehman MU, Wani K
Environmental toxicology34(5)610-625(2019 May)

Aplysin Protects Against Alcohol-Induced Liver Injury Via Alleviating Oxidative Damage and Modulating Endogenous Apoptosis-Related Genes Expression in Rats.
Ge N, Liang H, Zhao YY, Liu Y, Gong AJ, Zhang WL
Journal of food science83(10)2612-2621(2018 Oct)

Protective effect of acidic polysaccharide from Schisandra chinensis on acute ethanol-induced liver injury through reducing CYP2E1-dependent oxidative stress.
Yuan R, Tao X, Liang S, Pan Y, He L, Sun J, Wenbo J, Li X, Chen J, Wang C
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie99537-542(2018 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cyp2e1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp2e1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12909	NM_031543.1 Latest version!	Rattus norvegicus cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp2e1 ( NM_031543.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113731.1 Rattus norvegicus cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1), mRNA.

Price & Availability↑

CloneID	ORa12909
Clone ID Related Accession (Same CDS sequence)	NM_031543.1
Accession Version	NM_031543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573315200000
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 2E1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J02627.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J02627.1, FQ211086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGTTC TTGGCATCAC CATTGCCTTG CTGGTGTGGG TGGCCACCCT CCTCGTCATA 
TCCATCTGGA AGAAGATCTA TAACAGTTGG AACCTGCCCC CAGGACCTTT CCCTCTTCCC 
ATCCTTGGGA ACATTTTTCA GCTGGATTTG AAGGATATCC CCAAGTCTTT CACCAAGTTG 
GCAAAGCGCT TCGGGCCAGT GTTCACACTG CACCTTGGCT CAAGGCGCAT CGTGGTCCTG 
CATGGCTACA AGGCTGTCAA GGAGGTGCTA CTGAACCACA AGAATGAGTT TTCTGGACGG 
GGGGACATTC CTGTGTTCCA GGAGTACAAG AACAAGGGGA TTATTTTCAA TAATGGGCCC 
ACATGGAAGG ATGTGCGGAG GTTTTCCCTA AGCATTCTCC GAGACTGGGG AATGGGGAAA 
CAGGGTAATG AGGCCCGCAT CCAAAGGGAG GCGCAATTCC TGGTGGAGGA GCTCAAAAAG 
ACCAAAGGCC AGCCTTTTGA CCCCACATTT CTGATTGGCT GCGCACCCTG CAATGTCATT 
GCGGATATCC TCTTCAACAA ACGTTTCGAC TACAATGACA AGAAGTGTCT GAGGCTCATG 
AGTTTGTTCA ATGAAAACTT CTACCTGCTG AGCACCCCCT GGATCCAGCT TTACAATAAC 
TTTGCGGATT ATCTACGATA CCTACCTGGA AGCCATAGAA AAATCATGAA AAATGTGTCT 
GAAATAAAAC AGTACACACT TGAAAAAGCC AAGGAACACC TTCAGTCACT GGACATCAAC 
TGCGCCCGGG ATGTGACTGA CTGTCTCCTC ATAGAGATGG AGAAGGAAAA ACACAGCCAA 
GAACCCATGT ACACAATGGA AAATGTTTCT GTGACTTTGG CCGACCTGTT CTTTGCAGGA 
ACTGAGACCA CCAGCACAAC TCTGAGATAT GGGCTCCTGA TCCTCATGAA ATACCCAGAA 
ATTGAAGAGA AACTTCATGA AGAAATTGAC AGGGTTATTG GGCCAAGCCG CGTCCCTGCT 
GTCAGAGACA GACTGGATAT GCCCTACATG GATGCTGTGG TGCATGAGAT CCAGAGATTC 
ATCAATCTTG TCCCTTCCAA CCTACCCCAT GAAGCAACCA GAGATACTGT GTTCCAAGGA 
TATGTCATCC CCAAGGGTAC AGTTGTGATT CCAACTCTGG ACTCCCTCTT ATATGACAGC 
CATGAGTTTC CAGATCCAGA GAAGTTTAAA CCTGAGCATT TCCTGAATGA AAATGGGAAG 
TTCAAGTACA GTGACTATTT CAAGGCATTT TCTGCAGGAA AGCGTGTGTG TGTTGGAGAA 
GGCCTGGCCC GCATGGAATT GTTTCTGCTC CTGTCTGCTA TTCTGCAGCA TTTTAACCTG 
AAGTCTCTGG TTGACCCTAA GGATATCGAC CTCAGTCCTG TCACAGTTGG CTTTGGCAGT 
ATCCCACCCC AATTTAAACT CTGTGTCATT CCCCGTTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113731.1
CDS	13..1494
Translation	MAVLGITIALLVWVATLLVISIWKKIYNSWNLPPGPFPLPILGNIFQLDLKDIPKSFTKLAKRFGPVFTLHLGSRRIVVLHGYKAVKEVLLNHKNEFSGRGDIPVFQEYKNKGIIFNNGPTWKDVRRFSLSILRDWGMGKQGNEARIQREAQFLVEELKKTKGQPFDPTFLIGCAPCNVIADILFNKRFDYNDKKCLRLMSLFNENFYLLSTPWIQLYNNFADYLRYLPGSHRKIMKNVSEIKQYTLEKAKEHLQSLDINCARDVTDCLLIEMEKEKHSQEPMYTMENVSVTLADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRVPAVRDRLDMPYMDAVVHEIQRFINLVPSNLPHEATRDTVFQGYVIPKGTVVIPTLDSLLYDSHEFPDPEKFKPEHFLNENGKFKYSDYFKAFSAGKRVCVGEGLARMELFLLLSAILQHFNLKSLVDPKDIDLSPVTVGFGSIPPQFKLCVIPRS

Target ORF information:

RefSeq Version	NM_031543.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031543.1

ORF Insert Sequence:

ATGGCGGTTC TTGGCATCAC CATTGCCTTG CTGGTGTGGG TGGCCACCCT CCTCGTCATA 
TCCATCTGGA AGAAGATCTA TAACAGTTGG AACCTGCCCC CAGGACCTTT CCCTCTTCCC 
ATCCTTGGGA ACATTTTTCA GCTGGATTTG AAGGATATCC CCAAGTCTTT CACCAAGTTG 
GCAAAGCGCT TCGGGCCAGT GTTCACACTG CACCTTGGCT CAAGGCGCAT CGTGGTCCTG 
CATGGCTACA AGGCTGTCAA GGAGGTGCTA CTGAACCACA AGAATGAGTT TTCTGGACGG 
GGGGACATTC CTGTGTTCCA GGAGTACAAG AACAAGGGGA TTATTTTCAA TAATGGGCCC 
ACATGGAAGG ATGTGCGGAG GTTTTCCCTA AGCATTCTCC GAGACTGGGG AATGGGGAAA 
CAGGGTAATG AGGCCCGCAT CCAAAGGGAG GCGCAATTCC TGGTGGAGGA GCTCAAAAAG 
ACCAAAGGCC AGCCTTTTGA CCCCACATTT CTGATTGGCT GCGCACCCTG CAATGTCATT 
GCGGATATCC TCTTCAACAA ACGTTTCGAC TACAATGACA AGAAGTGTCT GAGGCTCATG 
AGTTTGTTCA ATGAAAACTT CTACCTGCTG AGCACCCCCT GGATCCAGCT TTACAATAAC 
TTTGCGGATT ATCTACGATA CCTACCTGGA AGCCATAGAA AAATCATGAA AAATGTGTCT 
GAAATAAAAC AGTACACACT TGAAAAAGCC AAGGAACACC TTCAGTCACT GGACATCAAC 
TGCGCCCGGG ATGTGACTGA CTGTCTCCTC ATAGAGATGG AGAAGGAAAA ACACAGCCAA 
GAACCCATGT ACACAATGGA AAATGTTTCT GTGACTTTGG CCGACCTGTT CTTTGCAGGA 
ACTGAGACCA CCAGCACAAC TCTGAGATAT GGGCTCCTGA TCCTCATGAA ATACCCAGAA 
ATTGAAGAGA AACTTCATGA AGAAATTGAC AGGGTTATTG GGCCAAGCCG CGTCCCTGCT 
GTCAGAGACA GACTGGATAT GCCCTACATG GATGCTGTGG TGCATGAGAT CCAGAGATTC 
ATCAATCTTG TCCCTTCCAA CCTACCCCAT GAAGCAACCA GAGATACTGT GTTCCAAGGA 
TATGTCATCC CCAAGGGTAC AGTTGTGATT CCAACTCTGG ACTCCCTCTT ATATGACAGC 
CATGAGTTTC CAGATCCAGA GAAGTTTAAA CCTGAGCATT TCCTGAATGA AAATGGGAAG 
TTCAAGTACA GTGACTATTT CAAGGCATTT TCTGCAGGAA AGCGTGTGTG TGTTGGAGAA 
GGCCTGGCCC GCATGGAATT GTTTCTGCTC CTGTCTGCTA TTCTGCAGCA TTTTAACCTG 
AAGTCTCTGG TTGACCCTAA GGATATCGAC CTCAGTCCTG TCACAGTTGG CTTTGGCAGT 
ATCCCACCCC AATTTAAACT CTGTGTCATT CCCCGTTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High CYP2E1 activity aggravates hepatofibrosis by limiting macrophage polarization towards the M2 phenotype.
Molecular carcinogenesis58(8)1481-1491(2019 08)
Guo YY,Xu C,Fang Y,Wang CE,Gao N,Wen Q,Qiao HL

Red meat intake, CYP2E1 and PPARγ polymorphisms, and colorectal cancer risk.
European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)28(4)304-310(2019 Jul)
Kim NH,Seol JE,Kim J,Lee BH,Hwang DY,Jeong J,Lee HJ,Ahn YO,Kim DH,Lee JE

Chemopreventive efficacy zingerone (4-[4-hydroxy-3-methylphenyl] butan-2-one) in experimental colon carcinogenesis in Wistar rats.
Environmental toxicology34(5)610-625(2019 May)
Ganaie MA,Al Saeedan A,Madhkali H,Jan BL,Khatlani T,Sheikh IA,Rehman MU,Wani K

Aplysin Protects Against Alcohol-Induced Liver Injury Via Alleviating Oxidative Damage and Modulating Endogenous Apoptosis-Related Genes Expression in Rats.
Journal of food science83(10)2612-2621(2018 Oct)
Ge N,Liang H,Zhao YY,Liu Y,Gong AJ,Zhang WL

Protective effect of acidic polysaccharide from Schisandra chinensis on acute ethanol-induced liver injury through reducing CYP2E1-dependent oxidative stress.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie99537-542(2018 Mar)
Yuan R,Tao X,Liang S,Pan Y,He L,Sun J,Wenbo J,Li X,Chen J,Wang C