Aspg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aspg
Entrez Gene ID	246266
Full Name	asparaginase
General protein information	Preferred Names asparaginase Names 60 kDa lysophospholipase asparaginase homolog lysophospholipase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32
Summary	displays lysophospholipase, asparaginase and platelet-activating factor acetylhydrolase activities [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_144750.2	NP_653351.1	60 kDa lysophospholipase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ASPG	XP_002696894.1
Homo sapiens (human)	ASPG	NP_001073933.2
Pan troglodytes (chimpanzee)	ASPG	XP_510194.2
Macaca mulatta (Rhesus monkey)	LOC701755	XP_002805268.1
Mus musculus (house mouse)	Aspg	NP_001074638.1
Canis lupus familiaris (dog)	ASPG	XP_005623920.1
Rattus norvegicus (Norway rat)	Aspg	NP_653351.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the transacylase activity of rat liver 60-kDa lysophospholipase-transacylase. Acyl transfer from the sn-2 to the sn-1 position.
Sugimoto H, Yamashita S
Biochimica et biophysica acta1438(2)264-72(1999 May)

Cloning and expression of cDNA encoding rat liver 60-kDa lysophospholipase containing an asparaginase-like region and ankyrin repeat.
Sugimoto H, Odani S, Yamashita S
The Journal of biological chemistry273(20)12536-42(1998 May)

Purification, characterization, and inhibition by phosphatidic acid of lysophospholipase transacylase from rat liver.
Sugimoto H, Yamashita S
The Journal of biological chemistry269(8)6252-8(1994 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Aspg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aspg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13681	NM_144750.2 Latest version!	Rattus norvegicus asparaginase (Aspg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aspg ( NM_144750.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_653351.1 Rattus norvegicus asparaginase (Aspg), mRNA.

Price & Availability↑

CloneID	ORa13681
Clone ID Related Accession (Same CDS sequence)	NM_144750.2
Accession Version	NM_144750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565366400000
Organism	Rattus norvegicus(Norway rat)
Product	60 kDa lysophospholipase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB009372.1. On Aug 31, 2012 this sequence version replaced NM_144750.1. ##Evidence-Data-START## Transcript exon combination :: AB009372.1, BC098655.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689598 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCGTG CTACCGGACC CGAGCAGCGG CTGCTGGCCA TCTACACTGG CGGCACCATC 
GGCATGCGGA GTGAAGGCGG AGTGCTGGTA CCTGGGAGAG GCCTGGCTGC TGTCCTGAGG 
ACACTTCACA TGCTCCACGA TGAAGAGTAT GCCAGAGCCC ACAGCCTGCC CGAGGATACG 
CTGGTGTTGC CCCCGGCCAG TTCTGACCAG AGGATCATCT ACAAGGTGCT GGAGTGCCAG 
CCACTCTTCG ACTCCAGTGA CATGACCATC ACTGAATGGG TTCAGATTGC CCAAACGATA 
GAGAGACACT ACACACAGTA CCAGGGCTTT GTGGTCATCC ACGGCACGGA CACCATGGCC 
TTTGCTGCGT CAGTGCTCTC TTTCATGCTG GAAAACCTGC AGAAACCGGT CATCCTCACA 
GGTGCCCAGG TTCCTATCCA CGAACTGTGG AGTGATGGCC GTGAGAACCT GCTGGGGGCT 
CTGCTCATGG CTGGCCAGTA CGTCATCCCT GAGGTCTGCC TGTTCTTCCA GAATCAGCTC 
TTTCGGGGCA ATCGGACAAC CAAGGTGGAC GCTCGGAGAT TTGCGGCCTT CTGCTCCCCT 
AACCTGCCCC CTCTAGCCAC TGTGGGCGCA GACGTAACAA TCAACCGTGA GCTGGTACGG 
AAGGCCAGTT GGAAGAGCCA CCTGGTGGTC CACAGCAACA TGGAGCCTGA TGTGGGCCTA 
CTGCGCCTCT ACCCTGGGAT TCCCGCCTCC CTGGTCCGGA CCTTCCTCCA GCCCCCACTG 
AAGGGCGTGG TCATGGAGAC CTTTGGCTCC GGGAATGGGC CCACCAAGCC GGACCTAATA 
CAGGAGCTCC GGGCGGCGGC CGAAAGAGGC CTGATCATTG TCAACTGTAC CCATTGCCTT 
CAGGGGGCTG TGACTTCCGA CTATGCACCT GGGATGGCCA TGGCAGGAGC CGGCATAATC 
TCAGGCTTCG ACATGACATC AGAAGCTGCC CTGGCCAAAC TGTCCTATGT GCTGGGCCAG 
CCAGGGCTGA GCCTGAGTGA CAGGAAGAAG CTGCTGGCCA AGGATCTTCG CGGGGAGATG 
ACACTGCCTA CAACAGATGA CTTGCTGGGA GATGACATGC TGGGCTGCAG GGCCACATGG 
CTTCTCAGTA TGAATGGCAG CCAGGATGCC GATGCCATGA AGGATGTTCT GCTGCCCGGC 
CTGGCCCTGG CTGCAGCTCA TGCTGGTGAC CTGGACACTC TGCAGGCCTT TGTGGAGCTG 
GGCAGAGACC TCAACCTGAA GGACTACAGT GGCCAGACGC CACTGCATGT GGCTGCACGG 
AGAGGGCATG CTTCAGTGGT CGCCATGCTA CTGCAGAAGG GTGTGGATGT GGATGCCTGT 
AATGAGGACG GTCAGAGCCC ACTGCTGCTG GCCGTGAGGG GCAGGCATCA GAGTGTCATC 
AGGCTGCTGA GGGCCGCTGG GGCTCACCTG TCTCCCCAGG AGCTGGAGGA CGTGGGGACA 
GAGCTGTGCA GGCTGGCATC AAGGGCGGAC AGCGAAGGCC TGCGGGCGTG GTGGCAGGCT 
GGGGCTGACC TAGGACAGCC GGACTATGAT GGACACTGTG CCCTGCAAGT TGCTGAAGCA 
GCAGGGAATG CAGATGTGGT GGCTCTGCTG CAGAGTCTTG AGGACAGGGT CAGCGCCCAG 
CCCCAGCCCC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653351.1
CDS	79..1773
Translation	MARATGPEQRLLAIYTGGTIGMRSEGGVLVPGRGLAAVLRTLHMLHDEEYARAHSLPEDTLVLPPASSDQRIIYKVLECQPLFDSSDMTITEWVQIAQTIERHYTQYQGFVVIHGTDTMAFAASVLSFMLENLQKPVILTGAQVPIHELWSDGRENLLGALLMAGQYVIPEVCLFFQNQLFRGNRTTKVDARRFAAFCSPNLPPLATVGADVTINRELVRKASWKSHLVVHSNMEPDVGLLRLYPGIPASLVRTFLQPPLKGVVMETFGSGNGPTKPDLIQELRAAAERGLIIVNCTHCLQGAVTSDYAPGMAMAGAGIISGFDMTSEAALAKLSYVLGQPGLSLSDRKKLLAKDLRGEMTLPTTDDLLGDDMLGCRATWLLSMNGSQDADAMKDVLLPGLALAAAHAGDLDTLQAFVELGRDLNLKDYSGQTPLHVAARRGHASVVAMLLQKGVDVDACNEDGQSPLLLAVRGRHQSVIRLLRAAGAHLSPQELEDVGTELCRLASRADSEGLRAWWQAGADLGQPDYDGHCALQVAEAAGNADVVALLQSLEDRVSAQPQPH

Target ORF information:

RefSeq Version	NM_144750.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus asparaginase (Aspg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144750.2

ORF Insert Sequence:

ATGGCGCGTG CTACCGGACC CGAGCAGCGG CTGCTGGCCA TCTACACTGG CGGCACCATC 
GGCATGCGGA GTGAAGGCGG AGTGCTGGTA CCTGGGAGAG GCCTGGCTGC TGTCCTGAGG 
ACACTTCACA TGCTCCACGA TGAAGAGTAT GCCAGAGCCC ACAGCCTGCC CGAGGATACG 
CTGGTGTTGC CCCCGGCCAG TTCTGACCAG AGGATCATCT ACAAGGTGCT GGAGTGCCAG 
CCACTCTTCG ACTCCAGTGA CATGACCATC ACTGAATGGG TTCAGATTGC CCAAACGATA 
GAGAGACACT ACACACAGTA CCAGGGCTTT GTGGTCATCC ACGGCACGGA CACCATGGCC 
TTTGCTGCGT CAGTGCTCTC TTTCATGCTG GAAAACCTGC AGAAACCGGT CATCCTCACA 
GGTGCCCAGG TTCCTATCCA CGAACTGTGG AGTGATGGCC GTGAGAACCT GCTGGGGGCT 
CTGCTCATGG CTGGCCAGTA CGTCATCCCT GAGGTCTGCC TGTTCTTCCA GAATCAGCTC 
TTTCGGGGCA ATCGGACAAC CAAGGTGGAC GCTCGGAGAT TTGCGGCCTT CTGCTCCCCT 
AACCTGCCCC CTCTAGCCAC TGTGGGCGCA GACGTAACAA TCAACCGTGA GCTGGTACGG 
AAGGCCAGTT GGAAGAGCCA CCTGGTGGTC CACAGCAACA TGGAGCCTGA TGTGGGCCTA 
CTGCGCCTCT ACCCTGGGAT TCCCGCCTCC CTGGTCCGGA CCTTCCTCCA GCCCCCACTG 
AAGGGCGTGG TCATGGAGAC CTTTGGCTCC GGGAATGGGC CCACCAAGCC GGACCTAATA 
CAGGAGCTCC GGGCGGCGGC CGAAAGAGGC CTGATCATTG TCAACTGTAC CCATTGCCTT 
CAGGGGGCTG TGACTTCCGA CTATGCACCT GGGATGGCCA TGGCAGGAGC CGGCATAATC 
TCAGGCTTCG ACATGACATC AGAAGCTGCC CTGGCCAAAC TGTCCTATGT GCTGGGCCAG 
CCAGGGCTGA GCCTGAGTGA CAGGAAGAAG CTGCTGGCCA AGGATCTTCG CGGGGAGATG 
ACACTGCCTA CAACAGATGA CTTGCTGGGA GATGACATGC TGGGCTGCAG GGCCACATGG 
CTTCTCAGTA TGAATGGCAG CCAGGATGCC GATGCCATGA AGGATGTTCT GCTGCCCGGC 
CTGGCCCTGG CTGCAGCTCA TGCTGGTGAC CTGGACACTC TGCAGGCCTT TGTGGAGCTG 
GGCAGAGACC TCAACCTGAA GGACTACAGT GGCCAGACGC CACTGCATGT GGCTGCACGG 
AGAGGGCATG CTTCAGTGGT CGCCATGCTA CTGCAGAAGG GTGTGGATGT GGATGCCTGT 
AATGAGGACG GTCAGAGCCC ACTGCTGCTG GCCGTGAGGG GCAGGCATCA GAGTGTCATC 
AGGCTGCTGA GGGCCGCTGG GGCTCACCTG TCTCCCCAGG AGCTGGAGGA CGTGGGGACA 
GAGCTGTGCA GGCTGGCATC AAGGGCGGAC AGCGAAGGCC TGCGGGCGTG GTGGCAGGCT 
GGGGCTGACC TAGGACAGCC GGACTATGAT GGACACTGTG CCCTGCAAGT TGCTGAAGCA 
GCAGGGAATG CAGATGTGGT GGCTCTGCTG CAGAGTCTTG AGGACAGGGT CAGCGCCCAG 
CCCCAGCCCC ATTAG