Hgf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hgf
Entrez Gene ID	24446
Full Name	hepatocyte growth factor
Synonyms	HPTA
General protein information	Preferred Names hepatocyte growth factor Names hepatocyte growth factor SF hepatopoeitin-A hepatopoietin-A scatter factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q12
Summary	plays a role in positive regulation of cell proliferation; may promote entry into the cell cycle [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006235979.3	XP_006236041.1	hepatocyte growth factor isoform X1
NM_017017.2	NP_058713.1	hepatocyte growth factor preproprotein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HGF	NP_001002964.1
Rattus norvegicus (Norway rat)	Hgf	NP_058713.1
Macaca mulatta (Rhesus monkey)	HGF	XP_001108226.2
Bos taurus (cattle)	HGF	NP_001026921.1
Homo sapiens (human)	HGF	NP_000592.3
Pan troglodytes (chimpanzee)	HGF	XP_519174.2
Mus musculus (house mouse)	Hgf	NP_034557.3
Xenopus tropicalis (tropical clawed frog)	hgf	XP_002933142.2
Gallus gallus (chicken)	HGF	NP_001025541.2
Danio rerio (zebrafish)	hgfa	NP_001013292.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of HGF and KGF gene silencing on vascular endothelial growth factor and its receptors in rat ultraviolet radiation‑induced corneal neovascularization.
He M, Han T, Wang Y, Wu YH, Qin WS, Du LZ, Zhao CQ
International journal of molecular medicine43(4)1888-1899(2019 Apr)

miR-26a modulates HGF and STAT3 effects on the kidney repair process in a glycerol-induced AKI model in rats.
Gattai PP, Maquigussa E, da Silva Novaes A, da Silva Ribeiro R, Varela VA, Ormanji MS, Boim MA
Journal of cellular biochemistry119(9)7757-7766(2018 09)

HGF Reduces Disease Severity and Inflammation by Attenuating the NF-κB Signaling in a Rat Model of Pulmonary Artery Hypertension.
Pang Y, Liang MT, Gong Y, Yang Y, Bu PL, Zhang M, Yao HC
Inflammation41(3)924-931(2018 Jun)

Endoplasmic reticulum stress restrains hepatocyte growth factor expression in hepatic stellate cells and rat acute liver failure model.
Liu Y, Pan X, Li S, Yu Y, Chen J, Yin J, Li G
Chemico-biological interactions27743-54(2017 Nov)

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Li J, Mao Q, He J, She H, Zhang Z, Yin C
Stem cell research & therapy8(1)55(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hgf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hgf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12609	NM_017017.2 Latest version!	Rattus norvegicus hepatocyte growth factor (Hgf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa26957	XM_006235979.3 Latest version!	Rattus norvegicus hepatocyte growth factor (Hgf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hgf ( NM_017017.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058713.1 Rattus norvegicus hepatocyte growth factor (Hgf), mRNA.

Price & Availability↑

CloneID	ORa12609
Clone ID Related Accession (Same CDS sequence)	NM_017017.2
Accession Version	NM_017017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Rattus norvegicus(Norway rat)
Product	hepatocyte growth factor preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D90102.1 and X54400.1. On Mar 24, 2010 this sequence version replaced NM_017017.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D90102.1, X54400.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATGTGGG GGACCAAACT TCTGCCGGTC CTGTTGCTGC AGCATGTCCT GCTGCACCTC 
CTCCTGCTTC CTGTCACCAT CCCCTATGCA GAAGGACAGA AGAAGAGAAG AAATACTCTT 
CATGAATTCA AAAAGTCAGC AAAAACTACT CTTACCAAGG AAGACCCATT AGTGAAGATT 
AAAACCAAAA AAGTGAACTC TGCAGATGAG TGTGCCAACA GGTGCATCAG AAACAAGGGC 
TTTCCATTCA CTTGCAAGGC CTTTGTTTTT GATAAGTCGA GAAAACGATG CTACTGGTAT 
CCTTTCAATA GTATGTCAAG TGGAGTGAAA AAAGGGTTTG GCCATGAATT TGACCTCTAT 
GAAAACAAAG ACTATATTAG AAATTGCATC ATTGGTAAAG GAGGCAGCTA TAAGGGGACA 
GTATCCATCA CTAAGAGTGG CATCAAGTGC CAGCCTTGGA ATTCCATGAT CCCCCATGAA 
CACAGCTTTT TGCCTTCGAG CTATCGCGGT AAAGACCTAC AGGAAAACTA CTGTCGAAAT 
CCTCGAGGGG AAGAAGGGGG ACCCTGGTGT TTCACAAGCA ATCCAGAGGT ACGCTACGAA 
GTCTGTGACA TTCCTCAGTG TTCAGAAGTT GAATGCATGA CCTGCAACGG TGAAAGCTAC 
AGAGGTCCCA TGGATCACAC AGAATCAGGC AAGACATGTC AGCGCTGGGA TCAGCAGACA 
CCACACCGGC ACAAATTCTT GCCGGAAAGA TATCCCGACA AGGGCTTTGA TGATAATTAT 
TGCCGCAATC CCGATGGCAA GCCGAGGCCA TGGTGCTACA CTCTTGACCC TGACACCCCT 
TGGGAGTATT GTGCAATTAA AATGTGCGCT CACAGTGCTG TGAATGAGAC TGATGTTCCC 
ATGGAAACAA CTGAATGTAT AAAAGGCCAA GGAGAAGGTT ACAGGGGAAC CACCAATACC 
ATTTGGAATG GAATTCCGTG TCAGCGTTGG GATTCGCAGT ACCCCCACAA GCATGACATC 
ACTCCCGAGA ACTTCAAATG CAAGGACCTT AGAGAAAATT ATTGCCGCAA TCCGGATGGG 
GCTGAATCAC CATGGTGTTT TACCACTGAT CCAAACATCC GAGTTGGTTA CTGCTCTCAA 
ATTCCCAAAT GTGACGTGTC AAGTGGACAA GATTGTTATC GTGGCAATGG GAAAAACTAC 
ATGGGCAACT TATCCAAAAC AAGGTCTGGA CTCACATGTT CCATGTGGGA CAAGAATATG 
GAGGATTTAC ACCGTCATAT CTTCTGGGAG CCAGACGCTA GCAAGTTGAC TAAGAATTAC 
TGCCGGAACC CCGATGACGA CGCCCATGGA CCTTGGTGCT ACACAGGGAA TCCTCTCGTT 
CCTTGGGATT ATTGCCCTAT TTCCCGTTGT GAAGGAGATA CTACACCTAC AATTGTCAAT 
TTGGACCATC CTGTAATATC CTGTGCCAAA ACAAAACAAC TGCGAGTTGT AAATGGCATT 
CCAACACAAA CAACAGTAGG GTGGATGGTT AGTTTGAAAT ACAGGAATAA ACACATCTGT 
GGGGGATCAT TGATAAAGGA AAGTTGGGTT CTTACTGCAA GGCAATGTTT TCCAGCTAGA 
AACAAAGACT TGAAAGACTA TGAAGCTTGG CTTGGAATCC ATGATGTCCA TGAGAGAGGC 
GAGGAGAAAC GCAAACAGAT CTTAAACATT TCCCAGCTAG TCTATGGACC TGAAGGCTCA 
GATTTGGTTT TACTGAAGCT TGCTCGCCCT GCAATCCTGG ATAACTTTGT CAGTACAATT 
GATTTACCTA GTTATGGCTG TACAATCCCT GAAAAGACTA CTTGCAGTAT TTACGGCTGG 
GGCTACACTG GATTGATCAA CGCAGATGGT TTATTACGAG TAGCTCATCT GTATATTATG 
GGGAATGAGA AATGCAGTCA GCACCATCAA GGCAAGGTGA CTTTGAATGA GTCTGAATTA 
TGTGCTGGGG CTGAAAAGAT TGGATCAGGA CCTTGTGAGG GAGATTATGG TGGCCCACTC 
ATTTGTGAAC AACACAAAAT GAGAATGGTT CTTGGTGTCA TTGTTCCTGG TCGTGGATGT 
GCCATCCCAA ATCGTCCTGG TATTTTTGTT CGAGTAGCAT ATTATGCAAA ATGGATACAC 
AAAGTAATTT TGACATACAA GTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058713.1
CDS	143..2329
Translation	MMWGTKLLPVLLLQHVLLHLLLLPVTIPYAEGQKKRRNTLHEFKKSAKTTLTKEDPLVKIKTKKVNSADECANRCIRNKGFPFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSAVNETDVPMETTECIKGQGEGYRGTTNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLTKNYCRNPDDDAHGPWCYTGNPLVPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

Target ORF information:

RefSeq Version	NM_017017.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hepatocyte growth factor (Hgf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017017.2

ORF Insert Sequence:

ATGATGTGGG GGACCAAACT TCTGCCGGTC CTGTTGCTGC AGCATGTCCT GCTGCACCTC 
CTCCTGCTTC CTGTCACCAT CCCCTATGCA GAAGGACAGA AGAAGAGAAG AAATACTCTT 
CATGAATTCA AAAAGTCAGC AAAAACTACT CTTACCAAGG AAGACCCATT AGTGAAGATT 
AAAACCAAAA AAGTGAACTC TGCAGATGAG TGTGCCAACA GGTGCATCAG AAACAAGGGC 
TTTCCATTCA CTTGCAAGGC CTTTGTTTTT GATAAGTCGA GAAAACGATG CTACTGGTAT 
CCTTTCAATA GTATGTCAAG TGGAGTGAAA AAAGGGTTTG GCCATGAATT TGACCTCTAT 
GAAAACAAAG ACTATATTAG AAATTGCATC ATTGGTAAAG GAGGCAGCTA TAAGGGGACA 
GTATCCATCA CTAAGAGTGG CATCAAGTGC CAGCCTTGGA ATTCCATGAT CCCCCATGAA 
CACAGCTTTT TGCCTTCGAG CTATCGCGGT AAAGACCTAC AGGAAAACTA CTGTCGAAAT 
CCTCGAGGGG AAGAAGGGGG ACCCTGGTGT TTCACAAGCA ATCCAGAGGT ACGCTACGAA 
GTCTGTGACA TTCCTCAGTG TTCAGAAGTT GAATGCATGA CCTGCAACGG TGAAAGCTAC 
AGAGGTCCCA TGGATCACAC AGAATCAGGC AAGACATGTC AGCGCTGGGA TCAGCAGACA 
CCACACCGGC ACAAATTCTT GCCGGAAAGA TATCCCGACA AGGGCTTTGA TGATAATTAT 
TGCCGCAATC CCGATGGCAA GCCGAGGCCA TGGTGCTACA CTCTTGACCC TGACACCCCT 
TGGGAGTATT GTGCAATTAA AATGTGCGCT CACAGTGCTG TGAATGAGAC TGATGTTCCC 
ATGGAAACAA CTGAATGTAT AAAAGGCCAA GGAGAAGGTT ACAGGGGAAC CACCAATACC 
ATTTGGAATG GAATTCCGTG TCAGCGTTGG GATTCGCAGT ACCCCCACAA GCATGACATC 
ACTCCCGAGA ACTTCAAATG CAAGGACCTT AGAGAAAATT ATTGCCGCAA TCCGGATGGG 
GCTGAATCAC CATGGTGTTT TACCACTGAT CCAAACATCC GAGTTGGTTA CTGCTCTCAA 
ATTCCCAAAT GTGACGTGTC AAGTGGACAA GATTGTTATC GTGGCAATGG GAAAAACTAC 
ATGGGCAACT TATCCAAAAC AAGGTCTGGA CTCACATGTT CCATGTGGGA CAAGAATATG 
GAGGATTTAC ACCGTCATAT CTTCTGGGAG CCAGACGCTA GCAAGTTGAC TAAGAATTAC 
TGCCGGAACC CCGATGACGA CGCCCATGGA CCTTGGTGCT ACACAGGGAA TCCTCTCGTT 
CCTTGGGATT ATTGCCCTAT TTCCCGTTGT GAAGGAGATA CTACACCTAC AATTGTCAAT 
TTGGACCATC CTGTAATATC CTGTGCCAAA ACAAAACAAC TGCGAGTTGT AAATGGCATT 
CCAACACAAA CAACAGTAGG GTGGATGGTT AGTTTGAAAT ACAGGAATAA ACACATCTGT 
GGGGGATCAT TGATAAAGGA AAGTTGGGTT CTTACTGCAA GGCAATGTTT TCCAGCTAGA 
AACAAAGACT TGAAAGACTA TGAAGCTTGG CTTGGAATCC ATGATGTCCA TGAGAGAGGC 
GAGGAGAAAC GCAAACAGAT CTTAAACATT TCCCAGCTAG TCTATGGACC TGAAGGCTCA 
GATTTGGTTT TACTGAAGCT TGCTCGCCCT GCAATCCTGG ATAACTTTGT CAGTACAATT 
GATTTACCTA GTTATGGCTG TACAATCCCT GAAAAGACTA CTTGCAGTAT TTACGGCTGG 
GGCTACACTG GATTGATCAA CGCAGATGGT TTATTACGAG TAGCTCATCT GTATATTATG 
GGGAATGAGA AATGCAGTCA GCACCATCAA GGCAAGGTGA CTTTGAATGA GTCTGAATTA 
TGTGCTGGGG CTGAAAAGAT TGGATCAGGA CCTTGTGAGG GAGATTATGG TGGCCCACTC 
ATTTGTGAAC AACACAAAAT GAGAATGGTT CTTGGTGTCA TTGTTCCTGG TCGTGGATGT 
GCCATCCCAA ATCGTCCTGG TATTTTTGTT CGAGTAGCAT ATTATGCAAA ATGGATACAC 
AAAGTAATTT TGACATACAA GTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hgf ( XM_006235979.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236041.1 Rattus norvegicus hepatocyte growth factor (Hgf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26957
Clone ID Related Accession (Same CDS sequence)	XM_006235979.3
Accession Version	XM_006235979.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	hepatocyte growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGGG GGACCAAACT TCTGCCGGTC CTGTTGCTGC AGCATGTCCT GCTGCACCTC 
CTCCTGCTTC CTGTCACCAT CCCCTATGCA GAAGGACAGA AGAAGAGAAG AAATACTCTT 
CATGAATTCA AAAAGTCAGC AAAAACTACT CTTACCAAGG AAGACCCATT AGTGAAGATT 
AAAACCAAAA AAGTGAACTC TGCAGATGAG TGTGCCAACA GGTGCATCAG AAACAAGGGC 
TTTCCATTCA CTTGCAAGGC CTTTGTTTTT GATAAGTCGA GAAAACGATG CTACTGGTAT 
CCTTTCAATA GTATGTCAAG TGGAGTGAAA AAAGGGTTTG GCCATGAATT TGACCTCTAT 
GAAAACAAAG ACTATATTAG AAATTGCATC ATTGGTAAAG GAGGCAGCTA TAAGGGGACA 
GTATCCATCA CTAAGAGTGG CATCAAGTGC CAGCCTTGGA ATTCCATGAT CCCCCATGAA 
CACAGCTATC GCGGTAAAGA CCTACAGGAA AACTACTGTC GAAATCCTCG AGGGGAAGAA 
GGGGGACCCT GGTGTTTCAC AAGCAATCCA GAGGTACGCT ACGAAGTCTG TGACATTCCT 
CAGTGTTCAG AAGTTGAATG CATGACCTGC AACGGTGAAA GCTACAGAGG TCCCATGGAT 
CACACAGAAT CAGGCAAGAC ATGTCAGCGC TGGGATCAGC AGACACCACA CCGGCACAAA 
TTCTTGCCGG AAAGATATCC CGACAAGGGC TTTGATGATA ATTATTGCCG CAATCCCGAT 
GGCAAGCCGA GGCCATGGTG CTACACTCTT GACCCTGACA CCCCTTGGGA GTATTGTGCA 
ATTAAAATGT GCGCTCACAG TGCTGTGAAT GAGACTGATG TTCCCATGGA AACAACTGAA 
TGTATAAAAG GCCAAGGAGA AGGTTACAGG GGAACCACCA ATACCATTTG GAATGGAATT 
CCGTGTCAGC GTTGGGATTC GCAGTACCCC CACAAGCATG ACATCACTCC CGAGAACTTC 
AAATGCAAGG ACCTTAGAGA AAATTATTGC CGCAATCCGG ATGGGGCTGA ATCACCATGG 
TGTTTTACCA CTGATCCAAA CATCCGAGTT GGTTACTGCT CTCAAATTCC CAAATGTGAC 
GTGTCAAGTG GACAAGATTG TTATCGTGGC AATGGGAAAA ACTACATGGG CAACTTATCC 
AAAACAAGGT CTGGACTCAC ATGTTCCATG TGGGACAAGA ATATGGAGGA TTTACACCGT 
CATATCTTCT GGGAGCCAGA CGCTAGCAAG TTGACTAAGA ATTACTGCCG GAACCCCGAT 
GACGACGCCC ATGGACCTTG GTGCTACACA GGGAATCCTC TCGTTCCTTG GGATTATTGC 
CCTATTTCCC GTTGTGAAGG AGATACTACA CCTACAATTG TCAATTTGGA CCATCCTGTA 
ATATCCTGTG CCAAAACAAA ACAACTGCGA GTTGTAAATG GCATTCCAAC ACAAACAACA 
GTAGGGTGGA TGGTTAGTTT GAAATACAGG AATAAACACA TCTGTGGGGG ATCATTGATA 
AAGGAAAGTT GGGTTCTTAC TGCAAGGCAA TGTTTTCCAG CTAGAAACAA AGACTTGAAA 
GACTATGAAG CTTGGCTTGG AATCCATGAT GTCCATGAGA GAGGCGAGGA GAAACGCAAA 
CAGATCTTAA ACATTTCCCA GCTAGTCTAT GGACCTGAAG GCTCAGATTT GGTTTTACTG 
AAGCTTGCTC GCCCTGCAAT CCTGGATAAC TTTGTCAGTA CAATTGATTT ACCTAGTTAT 
GGCTGTACAA TCCCTGAAAA GACTACTTGC AGTATTTACG GCTGGGGCTA CACTGGATTG 
ATCAACGCAG ATGGTTTATT ACGAGTAGCT CATCTGTATA TTATGGGGAA TGAGAAATGC 
AGTCAGCACC ATCAAGGCAA GGTGACTTTG AATGAGTCTG AATTATGTGC TGGGGCTGAA 
AAGATTGGAT CAGGACCTTG TGAGGGAGAT TATGGTGGCC CACTCATTTG TGAACAACAC 
AAAATGAGAA TGGTTCTTGG TGTCATTGTT CCTGGTCGTG GATGTGCCAT CCCAAATCGT 
CCTGGTATTT TTGTTCGAGT AGCATATTAT GCAAAATGGA TACACAAAGT AATTTTGACA 
TACAAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236041.1
CDS	237..2408
Translation	MMWGTKLLPVLLLQHVLLHLLLLPVTIPYAEGQKKRRNTLHEFKKSAKTTLTKEDPLVKIKTKKVNSADECANRCIRNKGFPFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSAVNETDVPMETTECIKGQGEGYRGTTNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLTKNYCRNPDDDAHGPWCYTGNPLVPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

Target ORF information:

RefSeq Version	XM_006235979.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hepatocyte growth factor (Hgf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235979.3

ORF Insert Sequence:

ATGATGTGGG GGACCAAACT TCTGCCGGTC CTGTTGCTGC AGCATGTCCT GCTGCACCTC 
CTCCTGCTTC CTGTCACCAT CCCCTATGCA GAAGGACAGA AGAAGAGAAG AAATACTCTT 
CATGAATTCA AAAAGTCAGC AAAAACTACT CTTACCAAGG AAGACCCATT AGTGAAGATT 
AAAACCAAAA AAGTGAACTC TGCAGATGAG TGTGCCAACA GGTGCATCAG AAACAAGGGC 
TTTCCATTCA CTTGCAAGGC CTTTGTTTTT GATAAGTCGA GAAAACGATG CTACTGGTAT 
CCTTTCAATA GTATGTCAAG TGGAGTGAAA AAAGGGTTTG GCCATGAATT TGACCTCTAT 
GAAAACAAAG ACTATATTAG AAATTGCATC ATTGGTAAAG GAGGCAGCTA TAAGGGGACA 
GTATCCATCA CTAAGAGTGG CATCAAGTGC CAGCCTTGGA ATTCCATGAT CCCCCATGAA 
CACAGCTATC GCGGTAAAGA CCTACAGGAA AACTACTGTC GAAATCCTCG AGGGGAAGAA 
GGGGGACCCT GGTGTTTCAC AAGCAATCCA GAGGTACGCT ACGAAGTCTG TGACATTCCT 
CAGTGTTCAG AAGTTGAATG CATGACCTGC AACGGTGAAA GCTACAGAGG TCCCATGGAT 
CACACAGAAT CAGGCAAGAC ATGTCAGCGC TGGGATCAGC AGACACCACA CCGGCACAAA 
TTCTTGCCGG AAAGATATCC CGACAAGGGC TTTGATGATA ATTATTGCCG CAATCCCGAT 
GGCAAGCCGA GGCCATGGTG CTACACTCTT GACCCTGACA CCCCTTGGGA GTATTGTGCA 
ATTAAAATGT GCGCTCACAG TGCTGTGAAT GAGACTGATG TTCCCATGGA AACAACTGAA 
TGTATAAAAG GCCAAGGAGA AGGTTACAGG GGAACCACCA ATACCATTTG GAATGGAATT 
CCGTGTCAGC GTTGGGATTC GCAGTACCCC CACAAGCATG ACATCACTCC CGAGAACTTC 
AAATGCAAGG ACCTTAGAGA AAATTATTGC CGCAATCCGG ATGGGGCTGA ATCACCATGG 
TGTTTTACCA CTGATCCAAA CATCCGAGTT GGTTACTGCT CTCAAATTCC CAAATGTGAC 
GTGTCAAGTG GACAAGATTG TTATCGTGGC AATGGGAAAA ACTACATGGG CAACTTATCC 
AAAACAAGGT CTGGACTCAC ATGTTCCATG TGGGACAAGA ATATGGAGGA TTTACACCGT 
CATATCTTCT GGGAGCCAGA CGCTAGCAAG TTGACTAAGA ATTACTGCCG GAACCCCGAT 
GACGACGCCC ATGGACCTTG GTGCTACACA GGGAATCCTC TCGTTCCTTG GGATTATTGC 
CCTATTTCCC GTTGTGAAGG AGATACTACA CCTACAATTG TCAATTTGGA CCATCCTGTA 
ATATCCTGTG CCAAAACAAA ACAACTGCGA GTTGTAAATG GCATTCCAAC ACAAACAACA 
GTAGGGTGGA TGGTTAGTTT GAAATACAGG AATAAACACA TCTGTGGGGG ATCATTGATA 
AAGGAAAGTT GGGTTCTTAC TGCAAGGCAA TGTTTTCCAG CTAGAAACAA AGACTTGAAA 
GACTATGAAG CTTGGCTTGG AATCCATGAT GTCCATGAGA GAGGCGAGGA GAAACGCAAA 
CAGATCTTAA ACATTTCCCA GCTAGTCTAT GGACCTGAAG GCTCAGATTT GGTTTTACTG 
AAGCTTGCTC GCCCTGCAAT CCTGGATAAC TTTGTCAGTA CAATTGATTT ACCTAGTTAT 
GGCTGTACAA TCCCTGAAAA GACTACTTGC AGTATTTACG GCTGGGGCTA CACTGGATTG 
ATCAACGCAG ATGGTTTATT ACGAGTAGCT CATCTGTATA TTATGGGGAA TGAGAAATGC 
AGTCAGCACC ATCAAGGCAA GGTGACTTTG AATGAGTCTG AATTATGTGC TGGGGCTGAA 
AAGATTGGAT CAGGACCTTG TGAGGGAGAT TATGGTGGCC CACTCATTTG TGAACAACAC 
AAAATGAGAA TGGTTCTTGG TGTCATTGTT CCTGGTCGTG GATGTGCCAT CCCAAATCGT 
CCTGGTATTT TTGTTCGAGT AGCATATTAT GCAAAATGGA TACACAAAGT AATTTTGACA 
TACAAGTTGT AA