Gusb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gusb
Entrez Gene ID	24434
Full Name	glucuronidase, beta
Synonyms	Ac2-223,Gus-s
General protein information	Preferred Names glucuronidase, beta Names beta-glucuronidase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12
Summary	catalyzes the conversion of a beta-D-glucuronoside and H2O to an alcohol and D-glucuronate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598254.1	XP_017453743.1	beta-glucuronidase isoform X1
XM_017598255.1	XP_017453744.1	beta-glucuronidase isoform X2
NM_017015.2	NP_058711.2	beta-glucuronidase precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gusb	NP_034498.1
Rattus norvegicus (Norway rat)	Gusb	NP_058711.2
Drosophila melanogaster (fruit fly)	CG15117	NP_725851.1
Caenorhabditis elegans (roundworm)	CELE_Y105E8B.9	NP_493548.1
Bos taurus (cattle)	GUSB	NP_001076905.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011859	XP_320660.4
Pan troglodytes (chimpanzee)	GUSB	XP_001138883.1
Homo sapiens (human)	GUSB	NP_000172.2
Canis lupus familiaris (dog)	GUSB	NP_001003191.1
Gallus gallus (chicken)	GUSB	NP_001034405.1
Danio rerio (zebrafish)	gusb	XP_700122.6
Xenopus tropicalis (tropical clawed frog)	gusb	XP_002935849.2
Danio rerio (zebrafish)	LOC100535236	XP_005165070.1

Gene Ontology
Bibliography

Related articles in PubMed

Human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum.
Thaysen-Andersen M, Venkatakrishnan V, Loke I, Laurini C, Diestel S, Parker BL, Packer NH
The Journal of biological chemistry290(14)8789-802(2015 Apr)

Residues essential for plasminogen binding by the cation-independent mannose 6-phosphate receptor.
Bohnsack RN, Patel M, Olson LJ, Twining SS, Dahms NM
Biochemistry49(3)635-44(2010 Jan)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Characterization of an MPS I-H knock-in mouse that carries a nonsense mutation analogous to the human IDUA-W402X mutation.
Wang D, Shukla C, Liu X, Schoeb TR, Clarke LA, Bedwell DM, Keeling KM
Molecular genetics and metabolism99(1)62-71(2010 Jan)

Cloning, sequencing, and expression of cDNA for human beta-glucuronidase.
Oshima A, Kyle JW, Miller RD, Hoffmann JW, Powell PP, Grubb JH, Sly WS, Tropak M, Guise KS, Gravel RA
Proceedings of the National Academy of Sciences of the United States of America84(3)685-9(1987 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gusb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gusb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa57777	XM_017598254.1 Latest version!	Rattus norvegicus glucuronidase, beta (Gusb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa57778	XM_017598255.1 Latest version!	Rattus norvegicus glucuronidase, beta (Gusb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa02519	NM_017015.2 Latest version!	Rattus norvegicus glucuronidase, beta (Gusb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gusb ( XM_017598254.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453743.1 Rattus norvegicus glucuronidase, beta (Gusb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57777
Clone ID Related Accession (Same CDS sequence)	XM_017598254.1
Accession Version	XM_017598254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	beta-glucuronidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGT GGTATCCCAA GGGTTACTTC GTCCAGGACA TAAGCTTCGA CTTCTTCAAC 
TATGCGGGGC TGCACCGGTC TGTGGTCCTG TACACCACCC CTACCACCTA TATCGATGAT 
ATCACTGTGA CCACTGACGT GGACCGGGAC GTTGGGCTGG TGAACTACTG GATTTCTGTC 
CAGGGCAGTG ACCATTTCCA GCTAGAAGTG CGTCTTCTGG ATGAGGATGG CAAAATTGTG 
GCCCGTGGAA CAGGGAATGA GGGTCAACTT AAGGTGCCGC GTGCCCACCT CTGGTGGCCT 
TACCTGATGC ATGAGCATCC AGCCTACCTG TACTCCTTGG AGGTGACGAT GACAACACCT 
GAGTCTGTGT CTGACTTCTA TACCCTCCCT GTCGGGATTC GAACAGTGGC TGTCACAAAG 
AGCAAGTTCC TCATAAATGG GAAGCCTTTC TACTTCCAAG GCGTCAACAA GCATGAGGAT 
TCAGATATCC GAGGGAGAGG CTTCGACTGG CCTCTGCTGA TAAAGGATTT CAACCTCCTC 
CGTTGGCTCG GGGCAAATTC CTTTCGTACC AGCCACTATC CCTACTCGGA GGAGGTACTT 
CAGCTCTGTG ACCGATATGG AATTGTGGTC ATCGATGAGT GTCCCGGTGT GGGCATCGTG 
CTGCCCCAGA GTTTTGGCAA CGTGTCTCTT CGGCACCACC TAGAGGTGAT GGACGAGCTG 
GTGCGCAGGG ACAAAAATCA CCCTGCGGTC GTGATGTGGT CTGTGGCCAA TGAGCCTGTC 
TCTTCTCTGA AACCTGCCGG ATATTACTTC AAGACGCTGA TCGCCCACAC CAAAGCCCTG 
GACCCCACCC GTCCTGTGAC CTTTGTGAGC AATACCAGAT ATGACGCAGA CCTGGGGGCC 
CCGTACGTGG ACGTGATTTG TGTGAACAGT TACTTATCCT GGTATCATGA CTACGGGCAT 
CTGGAGGTGA TTCAGCTGCA GCTGACTAGC CAGTTTGAGA ACTGGTATAA GATGTACCAG 
AAGCCAATTA TCCAGAGCGA GTATGGAGCA GACGCCGTCT CGGGGCTTCA TGAGGATCCA 
CCTCGCATGT TCAGTGAGGA GTACCAGACA GCTCTCCTGG AGAATTATCA TTTGATCCTG 
GATGAGAAAC GAAAAGAATA TGTCATCGGA GAGCTCATCT GGAATTTTGC TGACTTCATG 
ACGAACCAGT CACCACTGAG AGTAACAGGA AACAAGAAGG GGATCTTCAC TCGACAGAGA 
AACCCCAAGA TGGCAGCCTT CATTTTGCGA GAGAGATACT GGAGGATTGC CAACGAAACC 
AGAGGTTACG GTTCAGTGCC GAGGACCCAG TGTATGGGAA GCAGACCGTT CACATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453743.1
CDS	84..1463
Translation	MQLWYPKGYFVQDISFDFFNYAGLHRSVVLYTTPTTYIDDITVTTDVDRDVGLVNYWISVQGSDHFQLEVRLLDEDGKIVARGTGNEGQLKVPRAHLWWPYLMHEHPAYLYSLEVTMTTPESVSDFYTLPVGIRTVAVTKSKFLINGKPFYFQGVNKHEDSDIRGRGFDWPLLIKDFNLLRWLGANSFRTSHYPYSEEVLQLCDRYGIVVIDECPGVGIVLPQSFGNVSLRHHLEVMDELVRRDKNHPAVVMWSVANEPVSSLKPAGYYFKTLIAHTKALDPTRPVTFVSNTRYDADLGAPYVDVICVNSYLSWYHDYGHLEVIQLQLTSQFENWYKMYQKPIIQSEYGADAVSGLHEDPPRMFSEEYQTALLENYHLILDEKRKEYVIGELIWNFADFMTNQSPLRVTGNKKGIFTRQRNPKMAAFILRERYWRIANETRGYGSVPRTQCMGSRPFTF

Target ORF information:

RefSeq Version	XM_017598254.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucuronidase, beta (Gusb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598254.1

ORF Insert Sequence:

ATGCAGTTGT GGTATCCCAA GGGTTACTTC GTCCAGGACA TAAGCTTCGA CTTCTTCAAC 
TATGCGGGGC TGCACCGGTC TGTGGTCCTG TACACCACCC CTACCACCTA TATCGATGAT 
ATCACTGTGA CCACTGACGT GGACCGGGAC GTTGGGCTGG TGAACTACTG GATTTCTGTC 
CAGGGCAGTG ACCATTTCCA GCTAGAAGTG CGTCTTCTGG ATGAGGATGG CAAAATTGTG 
GCCCGTGGAA CAGGGAATGA GGGTCAACTT AAGGTGCCGC GTGCCCACCT CTGGTGGCCT 
TACCTGATGC ATGAGCATCC AGCCTACCTG TACTCCTTGG AGGTGACGAT GACAACACCT 
GAGTCTGTGT CTGACTTCTA TACCCTCCCT GTCGGGATTC GAACAGTGGC TGTCACAAAG 
AGCAAGTTCC TCATAAATGG GAAGCCTTTC TACTTCCAAG GCGTCAACAA GCATGAGGAT 
TCAGATATCC GAGGGAGAGG CTTCGACTGG CCTCTGCTGA TAAAGGATTT CAACCTCCTC 
CGTTGGCTCG GGGCAAATTC CTTTCGTACC AGCCACTATC CCTACTCGGA GGAGGTACTT 
CAGCTCTGTG ACCGATATGG AATTGTGGTC ATCGATGAGT GTCCCGGTGT GGGCATCGTG 
CTGCCCCAGA GTTTTGGCAA CGTGTCTCTT CGGCACCACC TAGAGGTGAT GGACGAGCTG 
GTGCGCAGGG ACAAAAATCA CCCTGCGGTC GTGATGTGGT CTGTGGCCAA TGAGCCTGTC 
TCTTCTCTGA AACCTGCCGG ATATTACTTC AAGACGCTGA TCGCCCACAC CAAAGCCCTG 
GACCCCACCC GTCCTGTGAC CTTTGTGAGC AATACCAGAT ATGACGCAGA CCTGGGGGCC 
CCGTACGTGG ACGTGATTTG TGTGAACAGT TACTTATCCT GGTATCATGA CTACGGGCAT 
CTGGAGGTGA TTCAGCTGCA GCTGACTAGC CAGTTTGAGA ACTGGTATAA GATGTACCAG 
AAGCCAATTA TCCAGAGCGA GTATGGAGCA GACGCCGTCT CGGGGCTTCA TGAGGATCCA 
CCTCGCATGT TCAGTGAGGA GTACCAGACA GCTCTCCTGG AGAATTATCA TTTGATCCTG 
GATGAGAAAC GAAAAGAATA TGTCATCGGA GAGCTCATCT GGAATTTTGC TGACTTCATG 
ACGAACCAGT CACCACTGAG AGTAACAGGA AACAAGAAGG GGATCTTCAC TCGACAGAGA 
AACCCCAAGA TGGCAGCCTT CATTTTGCGA GAGAGATACT GGAGGATTGC CAACGAAACC 
AGAGGTTACG GTTCAGTGCC GAGGACCCAG TGTATGGGAA GCAGACCGTT CACATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gusb ( XM_017598255.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453744.1 Rattus norvegicus glucuronidase, beta (Gusb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57778
Clone ID Related Accession (Same CDS sequence)	XM_017598255.1
Accession Version	XM_017598255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	beta-glucuronidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG GACACCGACA GGAGAGTGGT GTTGAGAATC AACAGCGCCC ATTACTATGC 
AGTTGTGGGC TGGTGAACTA CTGGATTTCT GTCCAGGGCA GTGACCATTT CCAGCTAGAA 
GTGCGTCTTC TGGATGAGGA TGGCAAAATT GTGGCCCGTG GAACAGGGAA TGAGGGTCAA 
CTTAAGGTGC CGCGTGCCCA CCTCTGGTGG CCTTACCTGA TGCATGAGCA TCCAGCCTAC 
CTGTACTCCT TGGAGGTGAC GATGACAACA CCTGAGTCTG TGTCTGACTT CTATACCCTC 
CCTGTCGGGA TTCGAACAGT GGCTGTCACA AAGAGCAAGT TCCTCATAAA TGGGAAGCCT 
TTCTACTTCC AAGGCGTCAA CAAGCATGAG GATTCAGATA TCCGAGGGAG AGGCTTCGAC 
TGGCCTCTGC TGATAAAGGA TTTCAACCTC CTCCGTTGGC TCGGGGCAAA TTCCTTTCGT 
ACCAGCCACT ATCCCTACTC GGAGGAGGTA CTTCAGCTCT GTGACCGATA TGGAATTGTG 
GTCATCGATG AGTGTCCCGG TGTGGGCATC GTGCTGCCCC AGAGTTTTGG CAACGTGTCT 
CTTCGGCACC ACCTAGAGGT GATGGACGAG CTGGTGCGCA GGGACAAAAA TCACCCTGCG 
GTCGTGATGT GGTCTGTGGC CAATGAGCCT GTCTCTTCTC TGAAACCTGC CGGATATTAC 
TTCAAGACGC TGATCGCCCA CACCAAAGCC CTGGACCCCA CCCGTCCTGT GACCTTTGTG 
AGCAATACCA GATATGACGC AGACCTGGGG GCCCCGTACG TGGACGTGAT TTGTGTGAAC 
AGTTACTTAT CCTGGTATCA TGACTACGGG CATCTGGAGG TGATTCAGCT GCAGCTGACT 
AGCCAGTTTG AGAACTGGTA TAAGATGTAC CAGAAGCCAA TTATCCAGAG CGAGTATGGA 
GCAGACGCCG TCTCGGGGCT TCATGAGGAT CCACCTCGCA TGTTCAGTGA GGAGTACCAG 
ACAGCTCTCC TGGAGAATTA TCATTTGATC CTGGATGAGA AACGAAAAGA ATATGTCATC 
GGAGAGCTCA TCTGGAATTT TGCTGACTTC ATGACGAACC AGTCACCACT GAGAGTAACA 
GGAAACAAGA AGGGGATCTT CACTCGACAG AGAAACCCCA AGATGGCAGC CTTCATTTTG 
CGAGAGAGAT ACTGGAGGAT TGCCAACGAA ACCAGAGGTT ACGGTTCAGT GCCGAGGACC 
CAGTGTATGG GAAGCAGACC GTTCACATTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453744.1
CDS	1..1293
Translation	MDPGHRQESGVENQQRPLLCSCGLVNYWISVQGSDHFQLEVRLLDEDGKIVARGTGNEGQLKVPRAHLWWPYLMHEHPAYLYSLEVTMTTPESVSDFYTLPVGIRTVAVTKSKFLINGKPFYFQGVNKHEDSDIRGRGFDWPLLIKDFNLLRWLGANSFRTSHYPYSEEVLQLCDRYGIVVIDECPGVGIVLPQSFGNVSLRHHLEVMDELVRRDKNHPAVVMWSVANEPVSSLKPAGYYFKTLIAHTKALDPTRPVTFVSNTRYDADLGAPYVDVICVNSYLSWYHDYGHLEVIQLQLTSQFENWYKMYQKPIIQSEYGADAVSGLHEDPPRMFSEEYQTALLENYHLILDEKRKEYVIGELIWNFADFMTNQSPLRVTGNKKGIFTRQRNPKMAAFILRERYWRIANETRGYGSVPRTQCMGSRPFTF

Target ORF information:

RefSeq Version	XM_017598255.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucuronidase, beta (Gusb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598255.1

ORF Insert Sequence:

ATGGACCCAG GACACCGACA GGAGAGTGGT GTTGAGAATC AACAGCGCCC ATTACTATGC 
AGTTGTGGGC TGGTGAACTA CTGGATTTCT GTCCAGGGCA GTGACCATTT CCAGCTAGAA 
GTGCGTCTTC TGGATGAGGA TGGCAAAATT GTGGCCCGTG GAACAGGGAA TGAGGGTCAA 
CTTAAGGTGC CGCGTGCCCA CCTCTGGTGG CCTTACCTGA TGCATGAGCA TCCAGCCTAC 
CTGTACTCCT TGGAGGTGAC GATGACAACA CCTGAGTCTG TGTCTGACTT CTATACCCTC 
CCTGTCGGGA TTCGAACAGT GGCTGTCACA AAGAGCAAGT TCCTCATAAA TGGGAAGCCT 
TTCTACTTCC AAGGCGTCAA CAAGCATGAG GATTCAGATA TCCGAGGGAG AGGCTTCGAC 
TGGCCTCTGC TGATAAAGGA TTTCAACCTC CTCCGTTGGC TCGGGGCAAA TTCCTTTCGT 
ACCAGCCACT ATCCCTACTC GGAGGAGGTA CTTCAGCTCT GTGACCGATA TGGAATTGTG 
GTCATCGATG AGTGTCCCGG TGTGGGCATC GTGCTGCCCC AGAGTTTTGG CAACGTGTCT 
CTTCGGCACC ACCTAGAGGT GATGGACGAG CTGGTGCGCA GGGACAAAAA TCACCCTGCG 
GTCGTGATGT GGTCTGTGGC CAATGAGCCT GTCTCTTCTC TGAAACCTGC CGGATATTAC 
TTCAAGACGC TGATCGCCCA CACCAAAGCC CTGGACCCCA CCCGTCCTGT GACCTTTGTG 
AGCAATACCA GATATGACGC AGACCTGGGG GCCCCGTACG TGGACGTGAT TTGTGTGAAC 
AGTTACTTAT CCTGGTATCA TGACTACGGG CATCTGGAGG TGATTCAGCT GCAGCTGACT 
AGCCAGTTTG AGAACTGGTA TAAGATGTAC CAGAAGCCAA TTATCCAGAG CGAGTATGGA 
GCAGACGCCG TCTCGGGGCT TCATGAGGAT CCACCTCGCA TGTTCAGTGA GGAGTACCAG 
ACAGCTCTCC TGGAGAATTA TCATTTGATC CTGGATGAGA AACGAAAAGA ATATGTCATC 
GGAGAGCTCA TCTGGAATTT TGCTGACTTC ATGACGAACC AGTCACCACT GAGAGTAACA 
GGAAACAAGA AGGGGATCTT CACTCGACAG AGAAACCCCA AGATGGCAGC CTTCATTTTG 
CGAGAGAGAT ACTGGAGGAT TGCCAACGAA ACCAGAGGTT ACGGTTCAGT GCCGAGGACC 
CAGTGTATGG GAAGCAGACC GTTCACATTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gusb ( NM_017015.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058711.2 Rattus norvegicus glucuronidase, beta (Gusb), mRNA.

Price & Availability↑

CloneID	ORa02519
Clone ID Related Accession (Same CDS sequence)	NM_017015.2
Accession Version	NM_017015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Rattus norvegicus(Norway rat)
Product	beta-glucuronidase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB792679.1, M13962.1 and CV795146.1. On Oct 16, 2007 this sequence version replaced NM_017015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M13962.1, Y00717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCCAA GACGGAGTGT GTGTTGGTTC GTACTGGGCC AGCTGCTGTG CAGCTGCGCG 
CTGGCGCTGC AGGGCGGGAT GCTGTTCCCG AAGGAGACCC CTTCGCGGGA ACTCAAGGTG 
CTGGACGGCC TGTGGAGCTT CCGTGCCGAT TACTCGAACA ATCGGTTGCA GGGCTTCGAG 
AAGCAGTGGT ACCGGCAGCC GCTACGGGAG TCGGGCCCAA CCTTGGACAT GCCGGTCCCT 
TCCAGCTTCA ATGACATCAC CCAAGAAGCA GAGCTTCGGA ACTTCATTGG CTGGGTGTGG 
TATGAACGGG AAGCAGTGCT TCCACAGCGA TGGACCCAGG ACACCGACAG GAGAGTGGTG 
TTGAGAATCA ACAGCGCCCA TTACTATGCA GTTGTGTGGG TGAATGGGAT TCATGTGGTG 
GAACATGAGG GAGGTCACCT CCCCTTTGAG GCTGACATCA CCAAGCTGGT CCAGAGTGGG 
CCCCTGACCA CCTTCCGGGT CACCATCGCC ATCAACAACA CACTGACCCC TTATACCCTT 
CCACCGGGGA CCATTGTCTA CAAGACTGAT CCTTCCATGT ATCCCAAGGG TTACTTCGTC 
CAGGACATAA GCTTCGACTT CTTCAACTAT GCGGGGCTGC ACCGGTCTGT GGTCCTGTAC 
ACCACCCCTA CCACCTATAT CGATGATATC ACTGTGACCA CTGACGTGGA CCGGGACGTT 
GGGCTGGTGA ACTACTGGAT TTCTGTCCAG GGCAGTGACC ATTTCCAGCT AGAAGTGCGT 
CTTCTGGATG AGGATGGCAA AATTGTGGCC CGTGGAACAG GGAATGAGGG TCAACTTAAG 
GTGCCGCGTG CCCACCTCTG GTGGCCTTAC CTGATGCATG AGCATCCAGC CTACCTGTAC 
TCCTTGGAGG TGACGATGAC AACACCTGAG TCTGTGTCTG ACTTCTATAC CCTCCCTGTC 
GGGATTCGAA CAGTGGCTGT CACAAAGAGC AAGTTCCTCA TAAATGGGAA GCCTTTCTAC 
TTCCAAGGCG TCAACAAGCA TGAGGATTCA GATATCCGAG GGAGAGGCTT CGACTGGCCT 
CTGCTGATAA AGGATTTCAA CCTCCTCCGT TGGCTCGGGG CAAATTCCTT TCGTACCAGC 
CACTATCCCT ACTCGGAGGA GGTACTTCAG CTCTGTGACC GATATGGAAT TGTGGTCATC 
GATGAGTGTC CCGGTGTGGG CATCGTGCTG CCCCAGAGTT TTGGCAACGT GTCTCTTCGG 
CACCACCTAG AGGTGATGGA CGAGCTGGTG CGCAGGGACA AAAATCACCC TGCGGTCGTG 
ATGTGGTCTG TGGCCAATGA GCCTGTCTCT TCTCTGAAAC CTGCCGGATA TTACTTCAAG 
ACGCTGATCG CCCACACCAA AGCCCTGGAC CCCACCCGTC CTGTGACCTT TGTGAGCAAT 
ACCAGATATG ACGCAGACAT GGGGGCCCCG TACGTGGACG TGATTTGTGT GAACAGTTAC 
TTATCCTGGT ATCATGACTA CGGGCATCTG GAGGTGATTC AGCTGCAGCT GACTAGCCAG 
TTTGAGAACT GGTATAAGAT GTACCAGAAG CCAATTATCC AGAGCGAGTA TGGAGCAGAC 
GCCGTCTCGG GGCTTCATGA GGATCCACCT CGCATGTTCA GTGAGGAGTA CCAGACAGCT 
CTCCTGGAGA ATTATCATTT GATCCTGGAT GAGAAACGAA AAGAATATGT CATCGGAGAG 
CTCATCTGGA ATTTTGCTGA CTTCATGACG AACCAGTCAC CACTGAGAGT AACAGGAAAC 
AAGAAGGGGA TCTTCACTCG ACAGAGAAAC CCCAAGATGG CAGCCTTCAT TTTGCGAGAG 
AGATACTGGA GGATTGCCAA CGAAACCAGA GGTTACGGTT CAGTGCCGAG GACCCAGTGT 
ATGGGAAGCA GACCGTTCAC ATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058711.2
CDS	37..1983
Translation	MSPRRSVCWFVLGQLLCSCALALQGGMLFPKETPSRELKVLDGLWSFRADYSNNRLQGFEKQWYRQPLRESGPTLDMPVPSSFNDITQEAELRNFIGWVWYEREAVLPQRWTQDTDRRVVLRINSAHYYAVVWVNGIHVVEHEGGHLPFEADITKLVQSGPLTTFRVTIAINNTLTPYTLPPGTIVYKTDPSMYPKGYFVQDISFDFFNYAGLHRSVVLYTTPTTYIDDITVTTDVDRDVGLVNYWISVQGSDHFQLEVRLLDEDGKIVARGTGNEGQLKVPRAHLWWPYLMHEHPAYLYSLEVTMTTPESVSDFYTLPVGIRTVAVTKSKFLINGKPFYFQGVNKHEDSDIRGRGFDWPLLIKDFNLLRWLGANSFRTSHYPYSEEVLQLCDRYGIVVIDECPGVGIVLPQSFGNVSLRHHLEVMDELVRRDKNHPAVVMWSVANEPVSSLKPAGYYFKTLIAHTKALDPTRPVTFVSNTRYDADMGAPYVDVICVNSYLSWYHDYGHLEVIQLQLTSQFENWYKMYQKPIIQSEYGADAVSGLHEDPPRMFSEEYQTALLENYHLILDEKRKEYVIGELIWNFADFMTNQSPLRVTGNKKGIFTRQRNPKMAAFILRERYWRIANETRGYGSVPRTQCMGSRPFTF

Target ORF information:

RefSeq Version	NM_017015.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucuronidase, beta (Gusb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017015.2

ORF Insert Sequence:

ATGTCCCCAA GACGGAGTGT GTGTTGGTTC GTACTGGGCC AGCTGCTGTG CAGCTGCGCG 
CTGGCGCTGC AGGGCGGGAT GCTGTTCCCG AAGGAGACCC CTTCGCGGGA ACTCAAGGTG 
CTGGACGGCC TGTGGAGCTT CCGTGCCGAT TACTCGAACA ATCGGTTGCA GGGCTTCGAG 
AAGCAGTGGT ACCGGCAGCC GCTACGGGAG TCGGGCCCAA CCTTGGACAT GCCGGTCCCT 
TCCAGCTTCA ATGACATCAC CCAAGAAGCA GAGCTTCGGA ACTTCATTGG CTGGGTGTGG 
TATGAACGGG AAGCAGTGCT TCCACAGCGA TGGACCCAGG ACACCGACAG GAGAGTGGTG 
TTGAGAATCA ACAGCGCCCA TTACTATGCA GTTGTGTGGG TGAATGGGAT TCATGTGGTG 
GAACATGAGG GAGGTCACCT CCCCTTTGAG GCTGACATCA CCAAGCTGGT CCAGAGTGGG 
CCCCTGACCA CCTTCCGGGT CACCATCGCC ATCAACAACA CACTGACCCC TTATACCCTT 
CCACCGGGGA CCATTGTCTA CAAGACTGAT CCTTCCATGT ATCCCAAGGG TTACTTCGTC 
CAGGACATAA GCTTCGACTT CTTCAACTAT GCGGGGCTGC ACCGGTCTGT GGTCCTGTAC 
ACCACCCCTA CCACCTATAT CGATGATATC ACTGTGACCA CTGACGTGGA CCGGGACGTT 
GGGCTGGTGA ACTACTGGAT TTCTGTCCAG GGCAGTGACC ATTTCCAGCT AGAAGTGCGT 
CTTCTGGATG AGGATGGCAA AATTGTGGCC CGTGGAACAG GGAATGAGGG TCAACTTAAG 
GTGCCGCGTG CCCACCTCTG GTGGCCTTAC CTGATGCATG AGCATCCAGC CTACCTGTAC 
TCCTTGGAGG TGACGATGAC AACACCTGAG TCTGTGTCTG ACTTCTATAC CCTCCCTGTC 
GGGATTCGAA CAGTGGCTGT CACAAAGAGC AAGTTCCTCA TAAATGGGAA GCCTTTCTAC 
TTCCAAGGCG TCAACAAGCA TGAGGATTCA GATATCCGAG GGAGAGGCTT CGACTGGCCT 
CTGCTGATAA AGGATTTCAA CCTCCTCCGT TGGCTCGGGG CAAATTCCTT TCGTACCAGC 
CACTATCCCT ACTCGGAGGA GGTACTTCAG CTCTGTGACC GATATGGAAT TGTGGTCATC 
GATGAGTGTC CCGGTGTGGG CATCGTGCTG CCCCAGAGTT TTGGCAACGT GTCTCTTCGG 
CACCACCTAG AGGTGATGGA CGAGCTGGTG CGCAGGGACA AAAATCACCC TGCGGTCGTG 
ATGTGGTCTG TGGCCAATGA GCCTGTCTCT TCTCTGAAAC CTGCCGGATA TTACTTCAAG 
ACGCTGATCG CCCACACCAA AGCCCTGGAC CCCACCCGTC CTGTGACCTT TGTGAGCAAT 
ACCAGATATG ACGCAGACAT GGGGGCCCCG TACGTGGACG TGATTTGTGT GAACAGTTAC 
TTATCCTGGT ATCATGACTA CGGGCATCTG GAGGTGATTC AGCTGCAGCT GACTAGCCAG 
TTTGAGAACT GGTATAAGAT GTACCAGAAG CCAATTATCC AGAGCGAGTA TGGAGCAGAC 
GCCGTCTCGG GGCTTCATGA GGATCCACCT CGCATGTTCA GTGAGGAGTA CCAGACAGCT 
CTCCTGGAGA ATTATCATTT GATCCTGGAT GAGAAACGAA AAGAATATGT CATCGGAGAG 
CTCATCTGGA ATTTTGCTGA CTTCATGACG AACCAGTCAC CACTGAGAGT AACAGGAAAC 
AAGAAGGGGA TCTTCACTCG ACAGAGAAAC CCCAAGATGG CAGCCTTCAT TTTGCGAGAG 
AGATACTGGA GGATTGCCAA CGAAACCAGA GGTTACGGTT CAGTGCCGAG GACCCAGTGT 
ATGGGAAGCA GACCGTTCAC ATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum.
The Journal of biological chemistry290(14)8789-802(2015 Apr)
Thaysen-Andersen M,Venkatakrishnan V,Loke I,Laurini C,Diestel S,Parker BL,Packer NH

Residues essential for plasminogen binding by the cation-independent mannose 6-phosphate receptor.
Biochemistry49(3)635-44(2010 Jan)
Bohnsack RN,Patel M,Olson LJ,Twining SS,Dahms NM

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Characterization of an MPS I-H knock-in mouse that carries a nonsense mutation analogous to the human IDUA-W402X mutation.
Molecular genetics and metabolism99(1)62-71(2010 Jan)
Wang D,Shukla C,Liu X,Schoeb TR,Clarke LA,Bedwell DM,Keeling KM

Cloning, sequencing, and expression of cDNA for human beta-glucuronidase.
Proceedings of the National Academy of Sciences of the United States of America84(3)685-9(1987 Feb)
Oshima A,Kyle JW,Miller RD,Hoffmann JW,Powell PP,Grubb JH,Sly WS,Tropak M,Guise KS,Gravel RA

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Gusb cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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