Nme1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nme1
Entrez Gene ID	191575
Full Name	NME/NM23 nucleoside diphosphate kinase 1
General protein information	Preferred Names NME/NM23 nucleoside diphosphate kinase 1 Names nucleoside diphosphate kinase A NDK A NDP kinase A NDP kinase beta expressed in non-metastatic cells 1, protein (NM23A) (nucleoside diphosphate kinase) metastasis inhibition factor NM23 non-metastatic cells 1, protein (NM23A) expressed in nucloside diphosphate kinase tumor metastatic process-associated protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26
Summary	the A isoform of the nucleoside diphosphate kinase; plays a role in nucleotide triphosphate metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596978.1	XP_017452467.1	nucleoside diphosphate kinase A isoform X1
XM_017596979.1	XP_017452468.1	nucleoside diphosphate kinase A isoform X1
NM_138548.2	NP_612557.1	nucleoside diphosphate kinase A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nme1	NP_612557.1
Canis lupus familiaris (dog)	NME1	NP_001019808.1
Arabidopsis thaliana (thale cress)	NDPK3	NP_192839.1
Pan troglodytes (chimpanzee)	NME1	NP_001191440.1
Homo sapiens (human)	NME1	NP_937818.1
Bos taurus (cattle)	NME1	NP_991387.2
Gallus gallus (chicken)	NME1	NP_001191690.1
Oryza sativa (rice)	Os05g0595400	NP_001056515.1
Arabidopsis thaliana (thale cress)	AT4G23895	NP_001190817.1
Danio rerio (zebrafish)	nme2b.1	NP_571001.1
Mus musculus (house mouse)	Nme1	NP_032730.1
Arabidopsis thaliana (thale cress)	AT4G23900	NP_567690.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kovács IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barabási AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Erythropoietin increases neuronal NDPKA expression, and NDPKA up-regulation as well as exogenous application protects cortical neurons from in vitro ischemia-related insults.
Teoh J, Boulos S, Chieng J, Knuckey NW, Meloni BP
Cellular and molecular neurobiology34(3)379-92(2014 Apr)

Nucleoside diphosphate kinase Nm23-M1 involves in oligodendroglial versus neuronal cell fate decision in vitro.
Owlanj H, Jie Yang H, Wei Feng Z
Differentiation; research in biological diversity84(4)281-93(2012 Nov)

Activity of nucleoside diphosphate kinase α (NDPK α) capable of binding to outer mitochondrial membrane accounts for less than 10% of total NDPK activity present in cytoplasm of liver cells.
Lipskaya TY, Voinova VV
Biochemistry. Biokhimiia77(6)593-602(2012 Jun)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nme1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nme1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01633	NM_138548.2 Latest version!	Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ORa01633	XM_017596979.1 Latest version!	Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ORa01633	XM_017596978.1 Latest version!	Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nme1 ( NM_138548.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612557.1 Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), mRNA.

Price & Availability↑

CloneID	ORa01633
Clone ID Related Accession (Same CDS sequence)	XM_017596978.1 , XM_017596979.1 , NM_138548.2
Accession Version	NM_138548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Rattus norvegicus(Norway rat)
Product	nucleoside diphosphate kinase A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D13374.1. On Aug 31, 2012 this sequence version replaced NM_138548.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D13374.1, CO570835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAACA GCGAGCGTAC CTTCATCGCC ATCAAGCCTG ATGGGGTCCA GCGGGGGCTT 
GTGGGAGAGA TCATCAAGCG ATTCGAGCAG AAGGGATTCC GCCTGGTTGG TTTGAAATTT 
ATACAGGCTT CAGAGGATCT TCTCAAGGAG CACTACATTG ACCTGAAGGA CCGCCCCTTC 
TTTTCTGGCC TGGTGAAGTA CATGCACTCA GGACCGGTGG TTGCTATGGT CTGGGAGGGA 
CTGAATGTTG TGAAGACAGG CCGGGTGATG CTTGGAGAGA CCAACCCCGC AGACTCTAAG 
CCTGGGACCA TACGAGGAGA CTTTTGCATT CAAGTTGGCA GGAACATCAT TCATGGCAGC 
GATTCTGTGG AGAGTGCGGA GAAGGAGATC AGTTTGTGGT TTCAGCCTGA GGAGCTGGTG 
GACTACAAGA GCTGTGCGCA GAACTGGATC TATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612557.1
CDS	111..569
Translation	MANSERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFIQASEDLLKEHYIDLKDRPFFSGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEISLWFQPEELVDYKSCAQNWIYE

Target ORF information:

RefSeq Version	NM_138548.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138548.2

ORF Insert Sequence:

ATGGCCAACA GCGAGCGTAC CTTCATCGCC ATCAAGCCTG ATGGGGTCCA GCGGGGGCTT 
GTGGGAGAGA TCATCAAGCG ATTCGAGCAG AAGGGATTCC GCCTGGTTGG TTTGAAATTT 
ATACAGGCTT CAGAGGATCT TCTCAAGGAG CACTACATTG ACCTGAAGGA CCGCCCCTTC 
TTTTCTGGCC TGGTGAAGTA CATGCACTCA GGACCGGTGG TTGCTATGGT CTGGGAGGGA 
CTGAATGTTG TGAAGACAGG CCGGGTGATG CTTGGAGAGA CCAACCCCGC AGACTCTAAG 
CCTGGGACCA TACGAGGAGA CTTTTGCATT CAAGTTGGCA GGAACATCAT TCATGGCAGC 
GATTCTGTGG AGAGTGCGGA GAAGGAGATC AGTTTGTGGT TTCAGCCTGA GGAGCTGGTG 
GACTACAAGA GCTGTGCGCA GAACTGGATC TATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nme1 ( XM_017596978.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452467.1 Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01633
Clone ID Related Accession (Same CDS sequence)	XM_017596978.1 , XM_017596979.1 , NM_138548.2
Accession Version	XM_017596978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	nucleoside diphosphate kinase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACA GCGAGCGTAC CTTCATCGCC ATCAAGCCTG ATGGGGTCCA GCGGGGGCTT 
GTGGGAGAGA TCATCAAGCG ATTCGAGCAG AAGGGATTCC GCCTGGTTGG TTTGAAATTT 
ATACAGGCTT CAGAGGATCT TCTCAAGGAG CACTACATTG ACCTGAAGGA CCGCCCCTTC 
TTTTCTGGCC TGGTGAAGTA CATGCACTCA GGACCGGTGG TTGCTATGGT CTGGGAGGGA 
CTGAATGTTG TGAAGACAGG CCGGGTGATG CTTGGAGAGA CCAACCCCGC AGACTCTAAG 
CCTGGGACCA TACGAGGAGA CTTTTGCATT CAAGTTGGCA GGAACATCAT TCATGGCAGC 
GATTCTGTGG AGAGTGCGGA GAAGGAGATC AGTTTGTGGT TTCAGCCTGA GGAGCTGGTG 
GACTACAAGA GCTGTGCGCA GAACTGGATC TATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452467.1
CDS	181..639
Translation	MANSERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFIQASEDLLKEHYIDLKDRPFFSGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEISLWFQPEELVDYKSCAQNWIYE

Target ORF information:

RefSeq Version	XM_017596978.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596978.1

ORF Insert Sequence:

ATGGCCAACA GCGAGCGTAC CTTCATCGCC ATCAAGCCTG ATGGGGTCCA GCGGGGGCTT 
GTGGGAGAGA TCATCAAGCG ATTCGAGCAG AAGGGATTCC GCCTGGTTGG TTTGAAATTT 
ATACAGGCTT CAGAGGATCT TCTCAAGGAG CACTACATTG ACCTGAAGGA CCGCCCCTTC 
TTTTCTGGCC TGGTGAAGTA CATGCACTCA GGACCGGTGG TTGCTATGGT CTGGGAGGGA 
CTGAATGTTG TGAAGACAGG CCGGGTGATG CTTGGAGAGA CCAACCCCGC AGACTCTAAG 
CCTGGGACCA TACGAGGAGA CTTTTGCATT CAAGTTGGCA GGAACATCAT TCATGGCAGC 
GATTCTGTGG AGAGTGCGGA GAAGGAGATC AGTTTGTGGT TTCAGCCTGA GGAGCTGGTG 
GACTACAAGA GCTGTGCGCA GAACTGGATC TATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nme1 ( XM_017596979.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452468.1 Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa01633
Clone ID Related Accession (Same CDS sequence)	XM_017596978.1 , XM_017596979.1 , NM_138548.2
Accession Version	XM_017596979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	nucleoside diphosphate kinase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACA GCGAGCGTAC CTTCATCGCC ATCAAGCCTG ATGGGGTCCA GCGGGGGCTT 
GTGGGAGAGA TCATCAAGCG ATTCGAGCAG AAGGGATTCC GCCTGGTTGG TTTGAAATTT 
ATACAGGCTT CAGAGGATCT TCTCAAGGAG CACTACATTG ACCTGAAGGA CCGCCCCTTC 
TTTTCTGGCC TGGTGAAGTA CATGCACTCA GGACCGGTGG TTGCTATGGT CTGGGAGGGA 
CTGAATGTTG TGAAGACAGG CCGGGTGATG CTTGGAGAGA CCAACCCCGC AGACTCTAAG 
CCTGGGACCA TACGAGGAGA CTTTTGCATT CAAGTTGGCA GGAACATCAT TCATGGCAGC 
GATTCTGTGG AGAGTGCGGA GAAGGAGATC AGTTTGTGGT TTCAGCCTGA GGAGCTGGTG 
GACTACAAGA GCTGTGCGCA GAACTGGATC TATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452468.1
CDS	69..527
Translation	MANSERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFIQASEDLLKEHYIDLKDRPFFSGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEISLWFQPEELVDYKSCAQNWIYE

Target ORF information:

RefSeq Version	XM_017596979.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NME/NM23 nucleoside diphosphate kinase 1 (Nme1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596979.1

ORF Insert Sequence:

ATGGCCAACA GCGAGCGTAC CTTCATCGCC ATCAAGCCTG ATGGGGTCCA GCGGGGGCTT 
GTGGGAGAGA TCATCAAGCG ATTCGAGCAG AAGGGATTCC GCCTGGTTGG TTTGAAATTT 
ATACAGGCTT CAGAGGATCT TCTCAAGGAG CACTACATTG ACCTGAAGGA CCGCCCCTTC 
TTTTCTGGCC TGGTGAAGTA CATGCACTCA GGACCGGTGG TTGCTATGGT CTGGGAGGGA 
CTGAATGTTG TGAAGACAGG CCGGGTGATG CTTGGAGAGA CCAACCCCGC AGACTCTAAG 
CCTGGGACCA TACGAGGAGA CTTTTGCATT CAAGTTGGCA GGAACATCAT TCATGGCAGC 
GATTCTGTGG AGAGTGCGGA GAAGGAGATC AGTTTGTGGT TTCAGCCTGA GGAGCTGGTG 
GACTACAAGA GCTGTGCGCA GAACTGGATC TATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kovács IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barabási AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Erythropoietin increases neuronal NDPKA expression, and NDPKA up-regulation as well as exogenous application protects cortical neurons from in vitro ischemia-related insults.
Cellular and molecular neurobiology34(3)379-92(2014 Apr)
Teoh J,Boulos S,Chieng J,Knuckey NW,Meloni BP

Nucleoside diphosphate kinase Nm23-M1 involves in oligodendroglial versus neuronal cell fate decision in vitro.
Differentiation; research in biological diversity84(4)281-93(2012 Nov)
Owlanj H,Jie Yang H,Wei Feng Z

Activity of nucleoside diphosphate kinase α (NDPK α) capable of binding to outer mitochondrial membrane accounts for less than 10% of total NDPK activity present in cytoplasm of liver cells.
Biochemistry. Biokhimiia77(6)593-602(2012 Jun)
Lipskaya TY,Voinova VV

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

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Nme1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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