Capn5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Capn5
Entrez Gene ID	171495
Full Name	calpain 5
Synonyms	Htra3
General protein information	Preferred Names calpain 5 Names calpain-5 high-temperature requirement factor A3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q32
Summary	human and mouse homologs are members of a subset of the calpain family of proteases that lack a calmodulin-like domain [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229729.3	XP_006229791.3	calpain-5 isoform X1
XM_008759673.2	XP_008757895.1	calpain-5 isoform X2
NM_134461.2	NP_604456.2	calpain-5
XM_006229731.3	XP_006229793.1	calpain-5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAPN5	XP_003313296.2
Mus musculus (house mouse)	Capn5	NP_031628.1
Danio rerio (zebrafish)	capn5a	NP_001073476.1
Canis lupus familiaris (dog)	CAPN5	XP_005633518.1
Danio rerio (zebrafish)	capn5b	XP_001345114.2
Caenorhabditis elegans (roundworm)	tra-3	NP_502751.1
Xenopus tropicalis (tropical clawed frog)	capn5	NP_001006736.1
Homo sapiens (human)	CAPN5	NP_004046.2
Macaca mulatta (Rhesus monkey)	LOC698077	XP_002799769.1
Bos taurus (cattle)	CAPN5	NP_001179823.1
Rattus norvegicus (Norway rat)	Capn5	NP_604456.1
Gallus gallus (chicken)	CAPN5	XP_417278.2

Gene Ontology
Bibliography

Related articles in PubMed

Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies.
Singh R, Brewer MK, Mashburn CB, Lou D, Bondada V, Graham B, Geddes JW
The Journal of biological chemistry289(28)19383-94(2014 Jul)

Calpain-5 mutations cause autoimmune uveitis, retinal neovascularization, and photoreceptor degeneration.
Mahajan VB, Skeie JM, Bassuk AG, Fingert JH, Braun TA, Daggett HT, Folk JC, Sheffield VC, Stone EM
PLoS genetics8(10)e1003001(2012)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

Evolutionary conservation of mammalian HTRA3 and its developmental regulation in the rat ovary.
Bowden M, Drummond AE, Salamonsen LA, Findlay JK, Nie G
Journal of experimental zoology. Part B, Molecular and developmental evolution312(7)701-13(2009 Nov)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Capn5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capn5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa23112	NM_134461.2 Latest version!	Rattus norvegicus calpain 5 (Capn5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa23112	XM_006229729.3 Latest version!	Rattus norvegicus calpain 5 (Capn5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa23112	XM_006229731.3 Latest version!	Rattus norvegicus calpain 5 (Capn5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa44338	XM_008759673.2 Latest version!	Rattus norvegicus calpain 5 (Capn5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Capn5 ( NM_134461.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_604456.2 Rattus norvegicus calpain 5 (Capn5), mRNA.

Price & Availability↑

CloneID	ORa23112
Clone ID Related Accession (Same CDS sequence)	XM_006229729.3 , NM_134461.2 , XM_006229731.3
Accession Version	NM_134461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Rattus norvegicus(Norway rat)
Product	calpain-5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131619.4 and AABR07071944.1. On or before Sep 4, 2015 this sequence version replaced XM_006229730.2, NM_134461.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FQ223532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTCCT GCACAAAGGC CTATGAAGAC CAGAATTACT CAGCTCTGAA GCGGGCCTGT 
CTGCGCAGGA AGGTGCTGTT CGAGGACCCC CACTTCCCTG CCACCGACGA CTCACTCTAC 
TATAAGGGTA CCCCAGGGCC CACAGTCAGG TGGAAGCGGC CTAAGGATAT CTGCGATGAT 
CCCCGGCTCT TCGTCGATGG CATCAGCTCG CATGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCCGCCTG CTCATCACTC GCCTCCCGGG AGTCACTCTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGAAC CCCGAGAAGC CCGACAGCTA TGCTGGCATC 
TTCCATTTCA ACTTCTGGCG CTTTGGGGAG TGGGTGGACG TGGTCGTCGA TGACCGGCTG 
CCCACAGTCA ACAACCAACT CATTTACTGC CACTCCAACT CGAAAAATGA GTTCTGGTGT 
GCCCTGGTGG AGAAGGCCTA TGCCAAGCTG GCAGGCTGTT ACCAGGCCCT GGACGGAGGC 
AACACAGCCG ATGCACTGGT GGATTTCACA GGCGGTGTTT CGGAACCCAT CGACCTGACC 
GAGGGGGACT TGGCCACAGA TGAGGCTAAG AGAAGCCAGC TCTTTGAACG TGTGTTGAAG 
GTGCACAGCA GAGGTGGGCT CATCAGTGCC TCCATCAAGG CTATGACAGC AGCTGACATG 
GAGACCCGTC TGGCCTGTGG TCTGGTGAAG GGCCATGCAT ATGCTGTCAC CGATGTGCGC 
AAGGTGCGCC TGGGCCATGG CCTGCTGGCC TTCTTCAAGT CAGAGAAGCT GGACATGATC 
CGTCTGAGGA ACCCCTGGGG CGAGCGGGAG TGGACCGGGC CCTGGAGTGA CACGTCAGAG 
GAATGGCAGA AAGTGAGCAA GAGTGAGAGG GAGAAAATGG GCGTGACCGT ACAGGACGAT 
GGTGAATTCT GGATGACCTT TGAGGACATG TGCCGATACT TCACCGACAT CATTAAGTGC 
CGCCTGATTA ATACGTCCTA CCTGAGCATC CACAAGACAT GGGAGGAGGC CCGGCTGCGT 
GGTGCCTGGA CGAGACATGA GGACCCCCAG CAGAACCGCA GTGGAGGCTG CATCAACCAC 
AAGGACACTT TCTTCCAGAA CCCACAGTAC ATATTTGAAG TCAAGAAGCC AGAAGATGAA 
GTGCTGATCT GCATCCAGCA GCGGCCCAAA CGCTCGACTC GCCGAGAGGG CAAAGGGGAG 
AATCTGGCCA TCGGCTTCGA CATCTATAAG GTGGAAGAGA ACCGCCAGTA CCGGATGCAC 
AGCCTGCAGC ATAAGGCCGC CAGCTCCATC TACATCAATT CTCGGAGTGT TTTCCTGCGG 
ACAGAGCTGC CTGAGGGCCG CTACGTCATC ATCCCTACCA CCTTTGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGCGT CTTCACAGAC GTTCCCTCCA ACTGCCGGGA GCTACGCCTG 
GATGAGCCCC CTCGTACCTG CTGGAGCTCC CTGTGTGGCT ACCCTCAGCA GGTGACCCAG 
GTCCATGTCC TGGGAGCTGC TGGCCTCAAG GACTCTTCCA CAGGAGCAAA CTCATATGTG 
ATCATCAAGT GTGAGGGTGA AAAGGTTCGC TCAGCTGTGC AGAGAGGGAC CTCGACGCCA 
GAGTACAATG TGAAAGGCAT CTTCTATCGC AAGAAACTGT CTCAGCCCAT CACTGTGCAG 
GTCTGGAATC ACCGAGTCCT GAAGGATGAA TTCCTGGGCC AGGTGCACCT GAAGACTGCC 
CCGGATGACC TGCAGGACCT GCACAGCCTC CATCTCCAGG ACCGCAGTGG CCGGCAGCCC 
AGCGACTTGC CAGGCATCGT CGCTGTGCGT GTCCTCTGCA GTGCCTCTCT CACGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_604456.2
CDS	200..2122
Translation	MFSCTKAYEDQNYSALKRACLRRKVLFEDPHFPATDDSLYYKGTPGPTVRWKRPKDICDDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVVVDDRLPTVNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRSQLFERVLKVHSRGGLISASIKAMTAADMETRLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLINTSYLSIHKTWEEARLRGAWTRHEDPQQNRSGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPRTCWSSLCGYPQQVTQVHVLGAAGLKDSSTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKTAPDDLQDLHSLHLQDRSGRQPSDLPGIVAVRVLCSASLTAV

Target ORF information:

RefSeq Version	NM_134461.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 5 (Capn5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134461.2

ORF Insert Sequence:

ATGTTCTCCT GCACAAAGGC CTATGAAGAC CAGAATTACT CAGCTCTGAA GCGGGCCTGT 
CTGCGCAGGA AGGTGCTGTT CGAGGACCCC CACTTCCCTG CCACCGACGA CTCACTCTAC 
TATAAGGGTA CCCCAGGGCC CACAGTCAGG TGGAAGCGGC CTAAGGATAT CTGCGATGAT 
CCCCGGCTCT TCGTCGATGG CATCAGCTCG CATGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCCGCCTG CTCATCACTC GCCTCCCGGG AGTCACTCTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGAAC CCCGAGAAGC CCGACAGCTA TGCTGGCATC 
TTCCATTTCA ACTTCTGGCG CTTTGGGGAG TGGGTGGACG TGGTCGTCGA TGACCGGCTG 
CCCACAGTCA ACAACCAACT CATTTACTGC CACTCCAACT CGAAAAATGA GTTCTGGTGT 
GCCCTGGTGG AGAAGGCCTA TGCCAAGCTG GCAGGCTGTT ACCAGGCCCT GGACGGAGGC 
AACACAGCCG ATGCACTGGT GGATTTCACA GGCGGTGTTT CGGAACCCAT CGACCTGACC 
GAGGGGGACT TGGCCACAGA TGAGGCTAAG AGAAGCCAGC TCTTTGAACG TGTGTTGAAG 
GTGCACAGCA GAGGTGGGCT CATCAGTGCC TCCATCAAGG CTATGACAGC AGCTGACATG 
GAGACCCGTC TGGCCTGTGG TCTGGTGAAG GGCCATGCAT ATGCTGTCAC CGATGTGCGC 
AAGGTGCGCC TGGGCCATGG CCTGCTGGCC TTCTTCAAGT CAGAGAAGCT GGACATGATC 
CGTCTGAGGA ACCCCTGGGG CGAGCGGGAG TGGACCGGGC CCTGGAGTGA CACGTCAGAG 
GAATGGCAGA AAGTGAGCAA GAGTGAGAGG GAGAAAATGG GCGTGACCGT ACAGGACGAT 
GGTGAATTCT GGATGACCTT TGAGGACATG TGCCGATACT TCACCGACAT CATTAAGTGC 
CGCCTGATTA ATACGTCCTA CCTGAGCATC CACAAGACAT GGGAGGAGGC CCGGCTGCGT 
GGTGCCTGGA CGAGACATGA GGACCCCCAG CAGAACCGCA GTGGAGGCTG CATCAACCAC 
AAGGACACTT TCTTCCAGAA CCCACAGTAC ATATTTGAAG TCAAGAAGCC AGAAGATGAA 
GTGCTGATCT GCATCCAGCA GCGGCCCAAA CGCTCGACTC GCCGAGAGGG CAAAGGGGAG 
AATCTGGCCA TCGGCTTCGA CATCTATAAG GTGGAAGAGA ACCGCCAGTA CCGGATGCAC 
AGCCTGCAGC ATAAGGCCGC CAGCTCCATC TACATCAATT CTCGGAGTGT TTTCCTGCGG 
ACAGAGCTGC CTGAGGGCCG CTACGTCATC ATCCCTACCA CCTTTGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGCGT CTTCACAGAC GTTCCCTCCA ACTGCCGGGA GCTACGCCTG 
GATGAGCCCC CTCGTACCTG CTGGAGCTCC CTGTGTGGCT ACCCTCAGCA GGTGACCCAG 
GTCCATGTCC TGGGAGCTGC TGGCCTCAAG GACTCTTCCA CAGGAGCAAA CTCATATGTG 
ATCATCAAGT GTGAGGGTGA AAAGGTTCGC TCAGCTGTGC AGAGAGGGAC CTCGACGCCA 
GAGTACAATG TGAAAGGCAT CTTCTATCGC AAGAAACTGT CTCAGCCCAT CACTGTGCAG 
GTCTGGAATC ACCGAGTCCT GAAGGATGAA TTCCTGGGCC AGGTGCACCT GAAGACTGCC 
CCGGATGACC TGCAGGACCT GCACAGCCTC CATCTCCAGG ACCGCAGTGG CCGGCAGCCC 
AGCGACTTGC CAGGCATCGT CGCTGTGCGT GTCCTCTGCA GTGCCTCTCT CACGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn5 ( XM_006229729.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229791.3 Rattus norvegicus calpain 5 (Capn5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23112
Clone ID Related Accession (Same CDS sequence)	XM_006229729.3 , NM_134461.2 , XM_006229731.3
Accession Version	XM_006229729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	calpain-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCT GCACAAAGGC CTATGAAGAC CAGAATTACT CAGCTCTGAA GCGGGCCTGT 
CTGCGCAGGA AGGTGCTGTT CGAGGACCCC CACTTCCCTG CCACCGACGA CTCACTCTAC 
TATAAGGGTA CCCCAGGGCC CACAGTCAGG TGGAAGCGGC CTAAGGATAT CTGCGATGAT 
CCCCGGCTCT TCGTCGATGG CATCAGCTCG CATGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCCGCCTG CTCATCACTC GCCTCCCGGG AGTCACTCTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGAAC CCCGAGAAGC CCGACAGCTA TGCTGGCATC 
TTCCATTTCA ACTTCTGGCG CTTTGGGGAG TGGGTGGACG TGGTCGTCGA TGACCGGCTG 
CCCACAGTCA ACAACCAACT CATTTACTGC CACTCCAACT CGAAAAATGA GTTCTGGTGT 
GCCCTGGTGG AGAAGGCCTA TGCCAAGCTG GCAGGCTGTT ACCAGGCCCT GGACGGAGGC 
AACACAGCCG ATGCACTGGT GGATTTCACA GGCGGTGTTT CGGAACCCAT CGACCTGACC 
GAGGGGGACT TGGCCACAGA TGAGGCTAAG AGAAGCCAGC TCTTTGAACG TGTGTTGAAG 
GTGCACAGCA GAGGTGGGCT CATCAGTGCC TCCATCAAGG CTATGACAGC AGCTGACATG 
GAGACCCGTC TGGCCTGTGG TCTGGTGAAG GGCCATGCAT ATGCTGTCAC CGATGTGCGC 
AAGGTGCGCC TGGGCCATGG CCTGCTGGCC TTCTTCAAGT CAGAGAAGCT GGACATGATC 
CGTCTGAGGA ACCCCTGGGG CGAGCGGGAG TGGACCGGGC CCTGGAGTGA CACGTCAGAG 
GAATGGCAGA AAGTGAGCAA GAGTGAGAGG GAGAAAATGG GCGTGACCGT ACAGGACGAT 
GGTGAATTCT GGATGACCTT TGAGGACATG TGCCGATACT TCACCGACAT CATTAAGTGC 
CGCCTGATTA ATACGTCCTA CCTGAGCATC CACAAGACAT GGGAGGAGGC CCGGCTGCGT 
GGTGCCTGGA CGAGACATGA GGACCCCCAG CAGAACCGCA GTGGAGGCTG CATCAACCAC 
AAGGACACTT TCTTCCAGAA CCCACAGTAC ATATTTGAAG TCAAGAAGCC AGAAGATGAA 
GTGCTGATCT GCATCCAGCA GCGGCCCAAA CGCTCGACTC GCCGAGAGGG CAAAGGGGAG 
AATCTGGCCA TCGGCTTCGA CATCTATAAG GTGGAAGAGA ACCGCCAGTA CCGGATGCAC 
AGCCTGCAGC ATAAGGCCGC CAGCTCCATC TACATCAATT CTCGGAGTGT TTTCCTGCGG 
ACAGAGCTGC CTGAGGGCCG CTACGTCATC ATCCCTACCA CCTTTGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGCGT CTTCACAGAC GTTCCCTCCA ACTGCCGGGA GCTACGCCTG 
GATGAGCCCC CTCGTACCTG CTGGAGCTCC CTGTGTGGCT ACCCTCAGCA GGTGACCCAG 
GTCCATGTCC TGGGAGCTGC TGGCCTCAAG GACTCTTCCA CAGGAGCAAA CTCATATGTG 
ATCATCAAGT GTGAGGGTGA AAAGGTTCGC TCAGCTGTGC AGAGAGGGAC CTCGACGCCA 
GAGTACAATG TGAAAGGCAT CTTCTATCGC AAGAAACTGT CTCAGCCCAT CACTGTGCAG 
GTCTGGAATC ACCGAGTCCT GAAGGATGAA TTCCTGGGCC AGGTGCACCT GAAGACTGCC 
CCGGATGACC TGCAGGACCT GCACAGCCTC CATCTCCAGG ACCGCAGTGG CCGGCAGCCC 
AGCGACTTGC CAGGCATCGT CGCTGTGCGT GTCCTCTGCA GTGCCTCTCT CACGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229791.3
CDS	205..2127
Translation	MFSCTKAYEDQNYSALKRACLRRKVLFEDPHFPATDDSLYYKGTPGPTVRWKRPKDICDDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVVVDDRLPTVNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRSQLFERVLKVHSRGGLISASIKAMTAADMETRLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLINTSYLSIHKTWEEARLRGAWTRHEDPQQNRSGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPRTCWSSLCGYPQQVTQVHVLGAAGLKDSSTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKTAPDDLQDLHSLHLQDRSGRQPSDLPGIVAVRVLCSASLTAV

Target ORF information:

RefSeq Version	XM_006229729.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 5 (Capn5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229729.3

ORF Insert Sequence:

ATGTTCTCCT GCACAAAGGC CTATGAAGAC CAGAATTACT CAGCTCTGAA GCGGGCCTGT 
CTGCGCAGGA AGGTGCTGTT CGAGGACCCC CACTTCCCTG CCACCGACGA CTCACTCTAC 
TATAAGGGTA CCCCAGGGCC CACAGTCAGG TGGAAGCGGC CTAAGGATAT CTGCGATGAT 
CCCCGGCTCT TCGTCGATGG CATCAGCTCG CATGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCCGCCTG CTCATCACTC GCCTCCCGGG AGTCACTCTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGAAC CCCGAGAAGC CCGACAGCTA TGCTGGCATC 
TTCCATTTCA ACTTCTGGCG CTTTGGGGAG TGGGTGGACG TGGTCGTCGA TGACCGGCTG 
CCCACAGTCA ACAACCAACT CATTTACTGC CACTCCAACT CGAAAAATGA GTTCTGGTGT 
GCCCTGGTGG AGAAGGCCTA TGCCAAGCTG GCAGGCTGTT ACCAGGCCCT GGACGGAGGC 
AACACAGCCG ATGCACTGGT GGATTTCACA GGCGGTGTTT CGGAACCCAT CGACCTGACC 
GAGGGGGACT TGGCCACAGA TGAGGCTAAG AGAAGCCAGC TCTTTGAACG TGTGTTGAAG 
GTGCACAGCA GAGGTGGGCT CATCAGTGCC TCCATCAAGG CTATGACAGC AGCTGACATG 
GAGACCCGTC TGGCCTGTGG TCTGGTGAAG GGCCATGCAT ATGCTGTCAC CGATGTGCGC 
AAGGTGCGCC TGGGCCATGG CCTGCTGGCC TTCTTCAAGT CAGAGAAGCT GGACATGATC 
CGTCTGAGGA ACCCCTGGGG CGAGCGGGAG TGGACCGGGC CCTGGAGTGA CACGTCAGAG 
GAATGGCAGA AAGTGAGCAA GAGTGAGAGG GAGAAAATGG GCGTGACCGT ACAGGACGAT 
GGTGAATTCT GGATGACCTT TGAGGACATG TGCCGATACT TCACCGACAT CATTAAGTGC 
CGCCTGATTA ATACGTCCTA CCTGAGCATC CACAAGACAT GGGAGGAGGC CCGGCTGCGT 
GGTGCCTGGA CGAGACATGA GGACCCCCAG CAGAACCGCA GTGGAGGCTG CATCAACCAC 
AAGGACACTT TCTTCCAGAA CCCACAGTAC ATATTTGAAG TCAAGAAGCC AGAAGATGAA 
GTGCTGATCT GCATCCAGCA GCGGCCCAAA CGCTCGACTC GCCGAGAGGG CAAAGGGGAG 
AATCTGGCCA TCGGCTTCGA CATCTATAAG GTGGAAGAGA ACCGCCAGTA CCGGATGCAC 
AGCCTGCAGC ATAAGGCCGC CAGCTCCATC TACATCAATT CTCGGAGTGT TTTCCTGCGG 
ACAGAGCTGC CTGAGGGCCG CTACGTCATC ATCCCTACCA CCTTTGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGCGT CTTCACAGAC GTTCCCTCCA ACTGCCGGGA GCTACGCCTG 
GATGAGCCCC CTCGTACCTG CTGGAGCTCC CTGTGTGGCT ACCCTCAGCA GGTGACCCAG 
GTCCATGTCC TGGGAGCTGC TGGCCTCAAG GACTCTTCCA CAGGAGCAAA CTCATATGTG 
ATCATCAAGT GTGAGGGTGA AAAGGTTCGC TCAGCTGTGC AGAGAGGGAC CTCGACGCCA 
GAGTACAATG TGAAAGGCAT CTTCTATCGC AAGAAACTGT CTCAGCCCAT CACTGTGCAG 
GTCTGGAATC ACCGAGTCCT GAAGGATGAA TTCCTGGGCC AGGTGCACCT GAAGACTGCC 
CCGGATGACC TGCAGGACCT GCACAGCCTC CATCTCCAGG ACCGCAGTGG CCGGCAGCCC 
AGCGACTTGC CAGGCATCGT CGCTGTGCGT GTCCTCTGCA GTGCCTCTCT CACGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn5 ( XM_006229731.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229793.1 Rattus norvegicus calpain 5 (Capn5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa23112
Clone ID Related Accession (Same CDS sequence)	XM_006229729.3 , NM_134461.2 , XM_006229731.3
Accession Version	XM_006229731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	calpain-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCCT GCACAAAGGC CTATGAAGAC CAGAATTACT CAGCTCTGAA GCGGGCCTGT 
CTGCGCAGGA AGGTGCTGTT CGAGGACCCC CACTTCCCTG CCACCGACGA CTCACTCTAC 
TATAAGGGTA CCCCAGGGCC CACAGTCAGG TGGAAGCGGC CTAAGGATAT CTGCGATGAT 
CCCCGGCTCT TCGTCGATGG CATCAGCTCG CATGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCCGCCTG CTCATCACTC GCCTCCCGGG AGTCACTCTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGAAC CCCGAGAAGC CCGACAGCTA TGCTGGCATC 
TTCCATTTCA ACTTCTGGCG CTTTGGGGAG TGGGTGGACG TGGTCGTCGA TGACCGGCTG 
CCCACAGTCA ACAACCAACT CATTTACTGC CACTCCAACT CGAAAAATGA GTTCTGGTGT 
GCCCTGGTGG AGAAGGCCTA TGCCAAGCTG GCAGGCTGTT ACCAGGCCCT GGACGGAGGC 
AACACAGCCG ATGCACTGGT GGATTTCACA GGCGGTGTTT CGGAACCCAT CGACCTGACC 
GAGGGGGACT TGGCCACAGA TGAGGCTAAG AGAAGCCAGC TCTTTGAACG TGTGTTGAAG 
GTGCACAGCA GAGGTGGGCT CATCAGTGCC TCCATCAAGG CTATGACAGC AGCTGACATG 
GAGACCCGTC TGGCCTGTGG TCTGGTGAAG GGCCATGCAT ATGCTGTCAC CGATGTGCGC 
AAGGTGCGCC TGGGCCATGG CCTGCTGGCC TTCTTCAAGT CAGAGAAGCT GGACATGATC 
CGTCTGAGGA ACCCCTGGGG CGAGCGGGAG TGGACCGGGC CCTGGAGTGA CACGTCAGAG 
GAATGGCAGA AAGTGAGCAA GAGTGAGAGG GAGAAAATGG GCGTGACCGT ACAGGACGAT 
GGTGAATTCT GGATGACCTT TGAGGACATG TGCCGATACT TCACCGACAT CATTAAGTGC 
CGCCTGATTA ATACGTCCTA CCTGAGCATC CACAAGACAT GGGAGGAGGC CCGGCTGCGT 
GGTGCCTGGA CGAGACATGA GGACCCCCAG CAGAACCGCA GTGGAGGCTG CATCAACCAC 
AAGGACACTT TCTTCCAGAA CCCACAGTAC ATATTTGAAG TCAAGAAGCC AGAAGATGAA 
GTGCTGATCT GCATCCAGCA GCGGCCCAAA CGCTCGACTC GCCGAGAGGG CAAAGGGGAG 
AATCTGGCCA TCGGCTTCGA CATCTATAAG GTGGAAGAGA ACCGCCAGTA CCGGATGCAC 
AGCCTGCAGC ATAAGGCCGC CAGCTCCATC TACATCAATT CTCGGAGTGT TTTCCTGCGG 
ACAGAGCTGC CTGAGGGCCG CTACGTCATC ATCCCTACCA CCTTTGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGCGT CTTCACAGAC GTTCCCTCCA ACTGCCGGGA GCTACGCCTG 
GATGAGCCCC CTCGTACCTG CTGGAGCTCC CTGTGTGGCT ACCCTCAGCA GGTGACCCAG 
GTCCATGTCC TGGGAGCTGC TGGCCTCAAG GACTCTTCCA CAGGAGCAAA CTCATATGTG 
ATCATCAAGT GTGAGGGTGA AAAGGTTCGC TCAGCTGTGC AGAGAGGGAC CTCGACGCCA 
GAGTACAATG TGAAAGGCAT CTTCTATCGC AAGAAACTGT CTCAGCCCAT CACTGTGCAG 
GTCTGGAATC ACCGAGTCCT GAAGGATGAA TTCCTGGGCC AGGTGCACCT GAAGACTGCC 
CCGGATGACC TGCAGGACCT GCACAGCCTC CATCTCCAGG ACCGCAGTGG CCGGCAGCCC 
AGCGACTTGC CAGGCATCGT CGCTGTGCGT GTCCTCTGCA GTGCCTCTCT CACGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229793.1
CDS	391..2313
Translation	MFSCTKAYEDQNYSALKRACLRRKVLFEDPHFPATDDSLYYKGTPGPTVRWKRPKDICDDPRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWNPEKPDSYAGIFHFNFWRFGEWVDVVVDDRLPTVNNQLIYCHSNSKNEFWCALVEKAYAKLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRSQLFERVLKVHSRGGLISASIKAMTAADMETRLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLINTSYLSIHKTWEEARLRGAWTRHEDPQQNRSGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPRTCWSSLCGYPQQVTQVHVLGAAGLKDSSTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKTAPDDLQDLHSLHLQDRSGRQPSDLPGIVAVRVLCSASLTAV

Target ORF information:

RefSeq Version	XM_006229731.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 5 (Capn5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229731.3

ORF Insert Sequence:

ATGTTCTCCT GCACAAAGGC CTATGAAGAC CAGAATTACT CAGCTCTGAA GCGGGCCTGT 
CTGCGCAGGA AGGTGCTGTT CGAGGACCCC CACTTCCCTG CCACCGACGA CTCACTCTAC 
TATAAGGGTA CCCCAGGGCC CACAGTCAGG TGGAAGCGGC CTAAGGATAT CTGCGATGAT 
CCCCGGCTCT TCGTCGATGG CATCAGCTCG CATGACCTGC ACCAGGGCCA GGTGGGCAAC 
TGCTGGTTTG TGGCCGCCTG CTCATCACTC GCCTCCCGGG AGTCACTCTG GCAAAAGGTC 
ATCCCAGACT GGAAGGAGCA GGAATGGAAC CCCGAGAAGC CCGACAGCTA TGCTGGCATC 
TTCCATTTCA ACTTCTGGCG CTTTGGGGAG TGGGTGGACG TGGTCGTCGA TGACCGGCTG 
CCCACAGTCA ACAACCAACT CATTTACTGC CACTCCAACT CGAAAAATGA GTTCTGGTGT 
GCCCTGGTGG AGAAGGCCTA TGCCAAGCTG GCAGGCTGTT ACCAGGCCCT GGACGGAGGC 
AACACAGCCG ATGCACTGGT GGATTTCACA GGCGGTGTTT CGGAACCCAT CGACCTGACC 
GAGGGGGACT TGGCCACAGA TGAGGCTAAG AGAAGCCAGC TCTTTGAACG TGTGTTGAAG 
GTGCACAGCA GAGGTGGGCT CATCAGTGCC TCCATCAAGG CTATGACAGC AGCTGACATG 
GAGACCCGTC TGGCCTGTGG TCTGGTGAAG GGCCATGCAT ATGCTGTCAC CGATGTGCGC 
AAGGTGCGCC TGGGCCATGG CCTGCTGGCC TTCTTCAAGT CAGAGAAGCT GGACATGATC 
CGTCTGAGGA ACCCCTGGGG CGAGCGGGAG TGGACCGGGC CCTGGAGTGA CACGTCAGAG 
GAATGGCAGA AAGTGAGCAA GAGTGAGAGG GAGAAAATGG GCGTGACCGT ACAGGACGAT 
GGTGAATTCT GGATGACCTT TGAGGACATG TGCCGATACT TCACCGACAT CATTAAGTGC 
CGCCTGATTA ATACGTCCTA CCTGAGCATC CACAAGACAT GGGAGGAGGC CCGGCTGCGT 
GGTGCCTGGA CGAGACATGA GGACCCCCAG CAGAACCGCA GTGGAGGCTG CATCAACCAC 
AAGGACACTT TCTTCCAGAA CCCACAGTAC ATATTTGAAG TCAAGAAGCC AGAAGATGAA 
GTGCTGATCT GCATCCAGCA GCGGCCCAAA CGCTCGACTC GCCGAGAGGG CAAAGGGGAG 
AATCTGGCCA TCGGCTTCGA CATCTATAAG GTGGAAGAGA ACCGCCAGTA CCGGATGCAC 
AGCCTGCAGC ATAAGGCCGC CAGCTCCATC TACATCAATT CTCGGAGTGT TTTCCTGCGG 
ACAGAGCTGC CTGAGGGCCG CTACGTCATC ATCCCTACCA CCTTTGAGCC AGGCCACACT 
GGCGAGTTCC TGCTCCGCGT CTTCACAGAC GTTCCCTCCA ACTGCCGGGA GCTACGCCTG 
GATGAGCCCC CTCGTACCTG CTGGAGCTCC CTGTGTGGCT ACCCTCAGCA GGTGACCCAG 
GTCCATGTCC TGGGAGCTGC TGGCCTCAAG GACTCTTCCA CAGGAGCAAA CTCATATGTG 
ATCATCAAGT GTGAGGGTGA AAAGGTTCGC TCAGCTGTGC AGAGAGGGAC CTCGACGCCA 
GAGTACAATG TGAAAGGCAT CTTCTATCGC AAGAAACTGT CTCAGCCCAT CACTGTGCAG 
GTCTGGAATC ACCGAGTCCT GAAGGATGAA TTCCTGGGCC AGGTGCACCT GAAGACTGCC 
CCGGATGACC TGCAGGACCT GCACAGCCTC CATCTCCAGG ACCGCAGTGG CCGGCAGCCC 
AGCGACTTGC CAGGCATCGT CGCTGTGCGT GTCCTCTGCA GTGCCTCTCT CACGGCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn5 ( XM_008759673.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757895.1 Rattus norvegicus calpain 5 (Capn5), transcript variant X3, mRNA.

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CloneID	ORa44338
Clone ID Related Accession (Same CDS sequence)	XM_008759673.2
Accession Version	XM_008759673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	calpain-5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCCG GCTCTTCGTC GATGGCATCA GCTCGCATGA CCTGCACCAG GGCCAGGTGG 
GCAACTGCTG GTTTGTGGCC GCCTGCTCAT CACTCGCCTC CCGGGAGTCA CTCTGGCAAA 
AGGCTGGCAG GCTGTTACCA GGCCCTGGAC GGAGGCAACA CAGCCGATGC ACTGGTGGAT 
TTCACAGGCG GTGTTTCGGA ACCCATCGAC CTGACCGAGG GGGACTTGGC CACAGATGAG 
GCTAAGAGAA GCCAGCTCTT TGAACGTGTG TTGAAGGTGC ACAGCAGAGG TGGGCTCATC 
AGTGCCTCCA TCAAGGCTAT GACAGCAGCT GACATGGAGA CCCGTCTGGC CTGTGGTCTG 
GTGAAGGGCC ATGCATATGC TGTCACCGAT GTGCGCAAGG TGCGCCTGGG CCATGGCCTG 
CTGGCCTTCT TCAAGTCAGA GAAGCTGGAC ATGATCCGTC TGAGGAACCC CTGGGGCGAG 
CGGGAGTGGA CCGGGCCCTG GAGTGACACG TCAGAGGAAT GGCAGAAAGT GAGCAAGAGT 
GAGAGGGAGA AAATGGGCGT GACCGTACAG GACGATGGTG AATTCTGGAT GACCTTTGAG 
GACATGTGCC GATACTTCAC CGACATCATT AAGTGCCGCC TGATTAATAC GTCCTACCTG 
AGCATCCACA AGACATGGGA GGAGGCCCGG CTGCGTGGTG CCTGGACGAG ACATGAGGAC 
CCCCAGCAGA ACCGCAGTGG AGGCTGCATC AACCACAAGG ACACTTTCTT CCAGAACCCA 
CAGTACATAT TTGAAGTCAA GAAGCCAGAA GATGAAGTGC TGATCTGCAT CCAGCAGCGG 
CCCAAACGCT CGACTCGCCG AGAGGGCAAA GGGGAGAATC TGGCCATCGG CTTCGACATC 
TATAAGGTGG AAGAGAACCG CCAGTACCGG ATGCACAGCC TGCAGCATAA GGCCGCCAGC 
TCCATCTACA TCAATTCTCG GAGTGTTTTC CTGCGGACAG AGCTGCCTGA GGGCCGCTAC 
GTCATCATCC CTACCACCTT TGAGCCAGGC CACACTGGCG AGTTCCTGCT CCGCGTCTTC 
ACAGACGTTC CCTCCAACTG CCGGGAGCTA CGCCTGGATG AGCCCCCTCG TACCTGCTGG 
AGCTCCCTGT GTGGCTACCC TCAGCAGGTG ACCCAGGTCC ATGTCCTGGG AGCTGCTGGC 
CTCAAGGACT CTTCCACAGG AGCAAACTCA TATGTGATCA TCAAGTGTGA GGGTGAAAAG 
GTTCGCTCAG CTGTGCAGAG AGGGACCTCG ACGCCAGAGT ACAATGTGAA AGGCATCTTC 
TATCGCAAGA AACTGTCTCA GCCCATCACT GTGCAGGTCT GGAATCACCG AGTCCTGAAG 
GATGAATTCC TGGGCCAGGT GCACCTGAAG ACTGCCCCGG ATGACCTGCA GGACCTGCAC 
AGCCTCCATC TCCAGGACCG CAGTGGCCGG CAGCCCAGCG ACTTGCCAGG CATCGTCGCT 
GTGCGTGTCC TCTGCAGTGC CTCTCTCACG GCTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757895.1
CDS	566..2104
Translation	MIPGSSSMASARMTCTRARWATAGLWPPAHHSPPGSHSGKRLAGCYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRSQLFERVLKVHSRGGLISASIKAMTAADMETRLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWTGPWSDTSEEWQKVSKSEREKMGVTVQDDGEFWMTFEDMCRYFTDIIKCRLINTSYLSIHKTWEEARLRGAWTRHEDPQQNRSGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTELPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPRTCWSSLCGYPQQVTQVHVLGAAGLKDSSTGANSYVIIKCEGEKVRSAVQRGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKTAPDDLQDLHSLHLQDRSGRQPSDLPGIVAVRVLCSASLTAV

Target ORF information:

RefSeq Version	XM_008759673.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 5 (Capn5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759673.2

ORF Insert Sequence:

ATGATCCCCG GCTCTTCGTC GATGGCATCA GCTCGCATGA CCTGCACCAG GGCCAGGTGG 
GCAACTGCTG GTTTGTGGCC GCCTGCTCAT CACTCGCCTC CCGGGAGTCA CTCTGGCAAA 
AGGCTGGCAG GCTGTTACCA GGCCCTGGAC GGAGGCAACA CAGCCGATGC ACTGGTGGAT 
TTCACAGGCG GTGTTTCGGA ACCCATCGAC CTGACCGAGG GGGACTTGGC CACAGATGAG 
GCTAAGAGAA GCCAGCTCTT TGAACGTGTG TTGAAGGTGC ACAGCAGAGG TGGGCTCATC 
AGTGCCTCCA TCAAGGCTAT GACAGCAGCT GACATGGAGA CCCGTCTGGC CTGTGGTCTG 
GTGAAGGGCC ATGCATATGC TGTCACCGAT GTGCGCAAGG TGCGCCTGGG CCATGGCCTG 
CTGGCCTTCT TCAAGTCAGA GAAGCTGGAC ATGATCCGTC TGAGGAACCC CTGGGGCGAG 
CGGGAGTGGA CCGGGCCCTG GAGTGACACG TCAGAGGAAT GGCAGAAAGT GAGCAAGAGT 
GAGAGGGAGA AAATGGGCGT GACCGTACAG GACGATGGTG AATTCTGGAT GACCTTTGAG 
GACATGTGCC GATACTTCAC CGACATCATT AAGTGCCGCC TGATTAATAC GTCCTACCTG 
AGCATCCACA AGACATGGGA GGAGGCCCGG CTGCGTGGTG CCTGGACGAG ACATGAGGAC 
CCCCAGCAGA ACCGCAGTGG AGGCTGCATC AACCACAAGG ACACTTTCTT CCAGAACCCA 
CAGTACATAT TTGAAGTCAA GAAGCCAGAA GATGAAGTGC TGATCTGCAT CCAGCAGCGG 
CCCAAACGCT CGACTCGCCG AGAGGGCAAA GGGGAGAATC TGGCCATCGG CTTCGACATC 
TATAAGGTGG AAGAGAACCG CCAGTACCGG ATGCACAGCC TGCAGCATAA GGCCGCCAGC 
TCCATCTACA TCAATTCTCG GAGTGTTTTC CTGCGGACAG AGCTGCCTGA GGGCCGCTAC 
GTCATCATCC CTACCACCTT TGAGCCAGGC CACACTGGCG AGTTCCTGCT CCGCGTCTTC 
ACAGACGTTC CCTCCAACTG CCGGGAGCTA CGCCTGGATG AGCCCCCTCG TACCTGCTGG 
AGCTCCCTGT GTGGCTACCC TCAGCAGGTG ACCCAGGTCC ATGTCCTGGG AGCTGCTGGC 
CTCAAGGACT CTTCCACAGG AGCAAACTCA TATGTGATCA TCAAGTGTGA GGGTGAAAAG 
GTTCGCTCAG CTGTGCAGAG AGGGACCTCG ACGCCAGAGT ACAATGTGAA AGGCATCTTC 
TATCGCAAGA AACTGTCTCA GCCCATCACT GTGCAGGTCT GGAATCACCG AGTCCTGAAG 
GATGAATTCC TGGGCCAGGT GCACCTGAAG ACTGCCCCGG ATGACCTGCA GGACCTGCAC 
AGCCTCCATC TCCAGGACCG CAGTGGCCGG CAGCCCAGCG ACTTGCCAGG CATCGTCGCT 
GTGCGTGTCC TCTGCAGTGC CTCTCTCACG GCTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies.
The Journal of biological chemistry289(28)19383-94(2014 Jul)
Singh R,Brewer MK,Mashburn CB,Lou D,Bondada V,Graham B,Geddes JW

Calpain-5 mutations cause autoimmune uveitis, retinal neovascularization, and photoreceptor degeneration.
PLoS genetics8(10)e1003001(2012)
Mahajan VB,Skeie JM,Bassuk AG,Fingert JH,Braun TA,Daggett HT,Folk JC,Sheffield VC,Stone EM

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

Evolutionary conservation of mammalian HTRA3 and its developmental regulation in the rat ovary.
Journal of experimental zoology. Part B, Molecular and developmental evolution312(7)701-13(2009 Nov)
Bowden M,Drummond AE,Salamonsen LA,Findlay JK,Nie G

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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Capn5 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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