Pcyt1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcyt1a
Entrez Gene ID	140544
Full Name	phosphate cytidylyltransferase 1, choline, alpha
Synonyms	CCT-alpha,CTP
General protein information	Preferred Names phosphate cytidylyltransferase 1, choline, alpha Names choline-phosphate cytidylyltransferase A CCT A CT A CTP:phosphocholine cytidylyl transferase CTP:phosphocholine cytidylyltransferase A CTP:phosphocholine cytidylyltransferase alpha choline phosphate cytidylyltransferase 1 alpha phosphate cytidylyltransferase 1, choline, alpha isoform phosphorylcholine transferase A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q22
Summary	catalyzes rate-limiting synthesis of phosphotidylcholine; plays a key role in cell membrane synthesis and in supply of lipid second messenger [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008768694.2	XP_008766916.1	choline-phosphate cytidylyltransferase A isoform X1
NM_078622.2	NP_511177.2	choline-phosphate cytidylyltransferase A
XM_006248444.3	XP_006248506.1	choline-phosphate cytidylyltransferase A isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002263	XP_003436073.1
Bos taurus (cattle)	PCYT1A	NP_001098522.1
Xenopus tropicalis (tropical clawed frog)	pcyt1a	XP_002934091.2
Homo sapiens (human)	PCYT1A	NP_005008.2
Mus musculus (house mouse)	Pcyt1a	NP_001156631.1
Drosophila melanogaster (fruit fly)	Cct2	NP_647622.1
Drosophila melanogaster (fruit fly)	Cct1	NP_728628.1
Canis lupus familiaris (dog)	PCYT1A	XP_535776.1
Danio rerio (zebrafish)	pcyt1aa	NP_001017634.1
Caenorhabditis elegans (roundworm)	pcyt-1	NP_001033539.1
Pan troglodytes (chimpanzee)	PCYT1A	XP_526433.2
Rattus norvegicus (Norway rat)	Pcyt1a	NP_511177.2
Gallus gallus (chicken)	PCYT1A	XP_422725.3
Caenorhabditis elegans (roundworm)	Y18H1A.11	NP_871893.1

Gene Ontology
Bibliography

Related articles in PubMed

Participation of prostaglandin D2 in the mobilization of the nuclear-localized CTP:phosphocholine cytidylyltransferase alpha in renal epithelial cells.
Favale NO, Pescio LG, Santacreu BJ, Márquez MG, Sterin-Speziale NB
Biochimica et biophysica acta1861(6)513-23(2016 Jun)

Structural basis for autoinhibition of CTP:phosphocholine cytidylyltransferase (CCT), the regulatory enzyme in phosphatidylcholine synthesis, by its membrane-binding amphipathic helix.
Lee J, Taneva SG, Holland BW, Tieleman DP, Cornell RB
The Journal of biological chemistry289(3)1742-55(2014 Jan)

ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.
Arsenault DJ, Yoo BH, Rosen KV, Ridgway ND
The Journal of biological chemistry288(1)633-43(2013 Jan)

A 22-mer segment in the structurally pliable regulatory domain of metazoan CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions.
Ding Z, Taneva SG, Huang HK, Campbell SA, Semenec L, Chen N, Cornell RB
The Journal of biological chemistry287(46)38980-91(2012 Nov)

CTP:phosphocholine cytidylyltransferase α (CCTα) and lamins alter nuclear membrane structure without affecting phosphatidylcholine synthesis.
Gehrig K, Ridgway ND
Biochimica et biophysica acta1811(6)377-85(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pcyt1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcyt1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12536	NM_078622.2 Latest version!	Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa12536	XM_008768694.2 Latest version!	Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa12536	XM_006248444.3 Latest version!	Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcyt1a ( NM_078622.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_511177.2 Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), mRNA.

Price & Availability↑

CloneID	ORa12536
Clone ID Related Accession (Same CDS sequence)	XM_008768694.2 , NM_078622.2 , XM_006248444.3
Accession Version	NM_078622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Rattus norvegicus(Norway rat)
Product	choline-phosphate cytidylyltransferase A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW919077.1, L13245.1, BF285251.1 and BQ780519.1. On Oct 6, 2004 this sequence version replaced NM_078622.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M36071.1, BC085713.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCAC AGAGTTCAGC TAAAGTCAAT TCAAGGAAGA GGAGGAAAGA GGTACCTGGC 
CCTAATGGAG CAACAGAGGA AGATGGAATT CCTTCCAAAG TGCAGCGCTG TGCAGTTGGT 
TTACGGCAGC CAGCTCCTTT TTCTGATGAA ATTGAAGTTG ACTTTAGTAA GCCCTATGTC 
AGGGTGACTA TGGAAGAAGC CTGCAGGGGA ACTCCTTGTG AGCGGCCTGT GAGAGTTTAT 
GCGGATGGAA TATTTGACTT ATTTCACTCT GGTCACGCCC GGGCTCTGAT GCAAGCAAAG 
AACCTTTTCC CTAATACATA TCTCATCGTG GGAGTCTGCA GTGATGAGCT AACGCACAAC 
TTCAAGGGCT TCACTGTGAT GAACGAAAAT GAACGTTACG ACGCGGTGCA GCACTGCCGT 
TACGTAGACG AGGTGGTGAG GAACGCCCCC TGGACTCTGA CGCCCGAGTT CCTGGCGGAG 
CACCGGATTG ATTTCGTCGC CCATGACGAT ATCCCCTACT CTTCGGCAGG GAGCGACGAT 
GTGTATAAGC ACATCAAGGA AGCAGGCATG TTTGCTCCCA CACAGAGGAC AGAAGGCATC 
TCCACATCAG ACATCATCAC CCGCATTGTC CGTGACTATG ATGTGTATGC AAGACGGAAC 
CTGCAGAGAG GCTACACTGC CAAGGAGCTC AATGTCAGCT TTATCAACGA AAAGAAATAC 
CACTTGCAAG AACGAGTTGA TAAGGTAAAG AAGAAAGTGA AAGATGTGGA GGAAAAGTCA 
AAAGAATTTG TGCAGAAAGT GGAGGAGAAG AGCATCGACC TCATCCAGAA GTGGGAGGAG 
AAGTCCCGAG AGTTCATTGG AAGTTTCCTG GAAATGTTTG GTCCAGAAGG AGCGCTGAAG 
CACATGCTGA AAGAGGGAAA AGGTCGGATG CTGCAGGCCA TCAGTCCCAA GCAGAGTCCC 
AGCAGCAGCC CTACTCATGA GCGCTCCCCC TCCCCCTCCT TTCGGTGGCC CTTCTCTGGC 
AAGACTTCCC CATCTTCCTC CCCAGCAAGT CTCTCTAGGT GCAAGGCTGT GACCTGTGAC 
ATCAGCGAGG ATGAAGAGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511177.2
CDS	104..1207
Translation	MDAQSSAKVNSRKRRKEVPGPNGATEEDGIPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEACRGTPCERPVRVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCRYVDEVVRNAPWTLTPEFLAEHRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEEKSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTHERSPSPSFRWPFSGKTSPSSSPASLSRCKAVTCDISEDEED

Target ORF information:

RefSeq Version	NM_078622.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078622.2

ORF Insert Sequence:

ATGGATGCAC AGAGTTCAGC TAAAGTCAAT TCAAGGAAGA GGAGGAAAGA GGTACCTGGC 
CCTAATGGAG CAACAGAGGA AGATGGAATT CCTTCCAAAG TGCAGCGCTG TGCAGTTGGT 
TTACGGCAGC CAGCTCCTTT TTCTGATGAA ATTGAAGTTG ACTTTAGTAA GCCCTATGTC 
AGGGTGACTA TGGAAGAAGC CTGCAGGGGA ACTCCTTGTG AGCGGCCTGT GAGAGTTTAT 
GCGGATGGAA TATTTGACTT ATTTCACTCT GGTCACGCCC GGGCTCTGAT GCAAGCAAAG 
AACCTTTTCC CTAATACATA TCTCATCGTG GGAGTCTGCA GTGATGAGCT AACGCACAAC 
TTCAAGGGCT TCACTGTGAT GAACGAAAAT GAACGTTACG ACGCGGTGCA GCACTGCCGT 
TACGTAGACG AGGTGGTGAG GAACGCCCCC TGGACTCTGA CGCCCGAGTT CCTGGCGGAG 
CACCGGATTG ATTTCGTCGC CCATGACGAT ATCCCCTACT CTTCGGCAGG GAGCGACGAT 
GTGTATAAGC ACATCAAGGA AGCAGGCATG TTTGCTCCCA CACAGAGGAC AGAAGGCATC 
TCCACATCAG ACATCATCAC CCGCATTGTC CGTGACTATG ATGTGTATGC AAGACGGAAC 
CTGCAGAGAG GCTACACTGC CAAGGAGCTC AATGTCAGCT TTATCAACGA AAAGAAATAC 
CACTTGCAAG AACGAGTTGA TAAGGTAAAG AAGAAAGTGA AAGATGTGGA GGAAAAGTCA 
AAAGAATTTG TGCAGAAAGT GGAGGAGAAG AGCATCGACC TCATCCAGAA GTGGGAGGAG 
AAGTCCCGAG AGTTCATTGG AAGTTTCCTG GAAATGTTTG GTCCAGAAGG AGCGCTGAAG 
CACATGCTGA AAGAGGGAAA AGGTCGGATG CTGCAGGCCA TCAGTCCCAA GCAGAGTCCC 
AGCAGCAGCC CTACTCATGA GCGCTCCCCC TCCCCCTCCT TTCGGTGGCC CTTCTCTGGC 
AAGACTTCCC CATCTTCCTC CCCAGCAAGT CTCTCTAGGT GCAAGGCTGT GACCTGTGAC 
ATCAGCGAGG ATGAAGAGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcyt1a ( XM_008768694.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766916.1 Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12536
Clone ID Related Accession (Same CDS sequence)	XM_008768694.2 , NM_078622.2 , XM_006248444.3
Accession Version	XM_008768694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	choline-phosphate cytidylyltransferase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAC AGAGTTCAGC TAAAGTCAAT TCAAGGAAGA GGAGGAAAGA GGTACCTGGC 
CCTAATGGAG CAACAGAGGA AGATGGAATT CCTTCCAAAG TGCAGCGCTG TGCAGTTGGT 
TTACGGCAGC CAGCTCCTTT TTCTGATGAA ATTGAAGTTG ACTTTAGTAA GCCCTATGTC 
AGGGTGACTA TGGAAGAAGC CTGCAGGGGA ACTCCTTGTG AGCGGCCTGT GAGAGTTTAT 
GCGGATGGAA TATTTGACTT ATTTCACTCT GGTCACGCCC GGGCTCTGAT GCAAGCAAAG 
AACCTTTTCC CTAATACATA TCTCATCGTG GGAGTCTGCA GTGATGAGCT AACGCACAAC 
TTCAAGGGCT TCACTGTGAT GAACGAAAAT GAACGTTACG ACGCGGTGCA GCACTGCCGT 
TACGTAGACG AGGTGGTGAG GAACGCCCCC TGGACTCTGA CGCCCGAGTT CCTGGCGGAG 
CACCGGATTG ATTTCGTCGC CCATGACGAT ATCCCCTACT CTTCGGCAGG GAGCGACGAT 
GTGTATAAGC ACATCAAGGA AGCAGGCATG TTTGCTCCCA CACAGAGGAC AGAAGGCATC 
TCCACATCAG ACATCATCAC CCGCATTGTC CGTGACTATG ATGTGTATGC AAGACGGAAC 
CTGCAGAGAG GCTACACTGC CAAGGAGCTC AATGTCAGCT TTATCAACGA AAAGAAATAC 
CACTTGCAAG AACGAGTTGA TAAGGTAAAG AAGAAAGTGA AAGATGTGGA GGAAAAGTCA 
AAAGAATTTG TGCAGAAAGT GGAGGAGAAG AGCATCGACC TCATCCAGAA GTGGGAGGAG 
AAGTCCCGAG AGTTCATTGG AAGTTTCCTG GAAATGTTTG GTCCAGAAGG AGCGCTGAAG 
CACATGCTGA AAGAGGGAAA AGGTCGGATG CTGCAGGCCA TCAGTCCCAA GCAGAGTCCC 
AGCAGCAGCC CTACTCATGA GCGCTCCCCC TCCCCCTCCT TTCGGTGGCC CTTCTCTGGC 
AAGACTTCCC CATCTTCCTC CCCAGCAAGT CTCTCTAGGT GCAAGGCTGT GACCTGTGAC 
ATCAGCGAGG ATGAAGAGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766916.1
CDS	457..1560
Translation	MDAQSSAKVNSRKRRKEVPGPNGATEEDGIPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEACRGTPCERPVRVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCRYVDEVVRNAPWTLTPEFLAEHRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEEKSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTHERSPSPSFRWPFSGKTSPSSSPASLSRCKAVTCDISEDEED

Target ORF information:

RefSeq Version	XM_008768694.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768694.2

ORF Insert Sequence:

ATGGATGCAC AGAGTTCAGC TAAAGTCAAT TCAAGGAAGA GGAGGAAAGA GGTACCTGGC 
CCTAATGGAG CAACAGAGGA AGATGGAATT CCTTCCAAAG TGCAGCGCTG TGCAGTTGGT 
TTACGGCAGC CAGCTCCTTT TTCTGATGAA ATTGAAGTTG ACTTTAGTAA GCCCTATGTC 
AGGGTGACTA TGGAAGAAGC CTGCAGGGGA ACTCCTTGTG AGCGGCCTGT GAGAGTTTAT 
GCGGATGGAA TATTTGACTT ATTTCACTCT GGTCACGCCC GGGCTCTGAT GCAAGCAAAG 
AACCTTTTCC CTAATACATA TCTCATCGTG GGAGTCTGCA GTGATGAGCT AACGCACAAC 
TTCAAGGGCT TCACTGTGAT GAACGAAAAT GAACGTTACG ACGCGGTGCA GCACTGCCGT 
TACGTAGACG AGGTGGTGAG GAACGCCCCC TGGACTCTGA CGCCCGAGTT CCTGGCGGAG 
CACCGGATTG ATTTCGTCGC CCATGACGAT ATCCCCTACT CTTCGGCAGG GAGCGACGAT 
GTGTATAAGC ACATCAAGGA AGCAGGCATG TTTGCTCCCA CACAGAGGAC AGAAGGCATC 
TCCACATCAG ACATCATCAC CCGCATTGTC CGTGACTATG ATGTGTATGC AAGACGGAAC 
CTGCAGAGAG GCTACACTGC CAAGGAGCTC AATGTCAGCT TTATCAACGA AAAGAAATAC 
CACTTGCAAG AACGAGTTGA TAAGGTAAAG AAGAAAGTGA AAGATGTGGA GGAAAAGTCA 
AAAGAATTTG TGCAGAAAGT GGAGGAGAAG AGCATCGACC TCATCCAGAA GTGGGAGGAG 
AAGTCCCGAG AGTTCATTGG AAGTTTCCTG GAAATGTTTG GTCCAGAAGG AGCGCTGAAG 
CACATGCTGA AAGAGGGAAA AGGTCGGATG CTGCAGGCCA TCAGTCCCAA GCAGAGTCCC 
AGCAGCAGCC CTACTCATGA GCGCTCCCCC TCCCCCTCCT TTCGGTGGCC CTTCTCTGGC 
AAGACTTCCC CATCTTCCTC CCCAGCAAGT CTCTCTAGGT GCAAGGCTGT GACCTGTGAC 
ATCAGCGAGG ATGAAGAGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcyt1a ( XM_006248444.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248506.1 Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12536
Clone ID Related Accession (Same CDS sequence)	XM_008768694.2 , NM_078622.2 , XM_006248444.3
Accession Version	XM_006248444.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	choline-phosphate cytidylyltransferase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248444.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAC AGAGTTCAGC TAAAGTCAAT TCAAGGAAGA GGAGGAAAGA GGTACCTGGC 
CCTAATGGAG CAACAGAGGA AGATGGAATT CCTTCCAAAG TGCAGCGCTG TGCAGTTGGT 
TTACGGCAGC CAGCTCCTTT TTCTGATGAA ATTGAAGTTG ACTTTAGTAA GCCCTATGTC 
AGGGTGACTA TGGAAGAAGC CTGCAGGGGA ACTCCTTGTG AGCGGCCTGT GAGAGTTTAT 
GCGGATGGAA TATTTGACTT ATTTCACTCT GGTCACGCCC GGGCTCTGAT GCAAGCAAAG 
AACCTTTTCC CTAATACATA TCTCATCGTG GGAGTCTGCA GTGATGAGCT AACGCACAAC 
TTCAAGGGCT TCACTGTGAT GAACGAAAAT GAACGTTACG ACGCGGTGCA GCACTGCCGT 
TACGTAGACG AGGTGGTGAG GAACGCCCCC TGGACTCTGA CGCCCGAGTT CCTGGCGGAG 
CACCGGATTG ATTTCGTCGC CCATGACGAT ATCCCCTACT CTTCGGCAGG GAGCGACGAT 
GTGTATAAGC ACATCAAGGA AGCAGGCATG TTTGCTCCCA CACAGAGGAC AGAAGGCATC 
TCCACATCAG ACATCATCAC CCGCATTGTC CGTGACTATG ATGTGTATGC AAGACGGAAC 
CTGCAGAGAG GCTACACTGC CAAGGAGCTC AATGTCAGCT TTATCAACGA AAAGAAATAC 
CACTTGCAAG AACGAGTTGA TAAGGTAAAG AAGAAAGTGA AAGATGTGGA GGAAAAGTCA 
AAAGAATTTG TGCAGAAAGT GGAGGAGAAG AGCATCGACC TCATCCAGAA GTGGGAGGAG 
AAGTCCCGAG AGTTCATTGG AAGTTTCCTG GAAATGTTTG GTCCAGAAGG AGCGCTGAAG 
CACATGCTGA AAGAGGGAAA AGGTCGGATG CTGCAGGCCA TCAGTCCCAA GCAGAGTCCC 
AGCAGCAGCC CTACTCATGA GCGCTCCCCC TCCCCCTCCT TTCGGTGGCC CTTCTCTGGC 
AAGACTTCCC CATCTTCCTC CCCAGCAAGT CTCTCTAGGT GCAAGGCTGT GACCTGTGAC 
ATCAGCGAGG ATGAAGAGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248506.1
CDS	262..1365
Translation	MDAQSSAKVNSRKRRKEVPGPNGATEEDGIPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEACRGTPCERPVRVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCRYVDEVVRNAPWTLTPEFLAEHRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEEKSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTHERSPSPSFRWPFSGKTSPSSSPASLSRCKAVTCDISEDEED

Target ORF information:

RefSeq Version	XM_006248444.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphate cytidylyltransferase 1, choline, alpha (Pcyt1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248444.3

ORF Insert Sequence:

ATGGATGCAC AGAGTTCAGC TAAAGTCAAT TCAAGGAAGA GGAGGAAAGA GGTACCTGGC 
CCTAATGGAG CAACAGAGGA AGATGGAATT CCTTCCAAAG TGCAGCGCTG TGCAGTTGGT 
TTACGGCAGC CAGCTCCTTT TTCTGATGAA ATTGAAGTTG ACTTTAGTAA GCCCTATGTC 
AGGGTGACTA TGGAAGAAGC CTGCAGGGGA ACTCCTTGTG AGCGGCCTGT GAGAGTTTAT 
GCGGATGGAA TATTTGACTT ATTTCACTCT GGTCACGCCC GGGCTCTGAT GCAAGCAAAG 
AACCTTTTCC CTAATACATA TCTCATCGTG GGAGTCTGCA GTGATGAGCT AACGCACAAC 
TTCAAGGGCT TCACTGTGAT GAACGAAAAT GAACGTTACG ACGCGGTGCA GCACTGCCGT 
TACGTAGACG AGGTGGTGAG GAACGCCCCC TGGACTCTGA CGCCCGAGTT CCTGGCGGAG 
CACCGGATTG ATTTCGTCGC CCATGACGAT ATCCCCTACT CTTCGGCAGG GAGCGACGAT 
GTGTATAAGC ACATCAAGGA AGCAGGCATG TTTGCTCCCA CACAGAGGAC AGAAGGCATC 
TCCACATCAG ACATCATCAC CCGCATTGTC CGTGACTATG ATGTGTATGC AAGACGGAAC 
CTGCAGAGAG GCTACACTGC CAAGGAGCTC AATGTCAGCT TTATCAACGA AAAGAAATAC 
CACTTGCAAG AACGAGTTGA TAAGGTAAAG AAGAAAGTGA AAGATGTGGA GGAAAAGTCA 
AAAGAATTTG TGCAGAAAGT GGAGGAGAAG AGCATCGACC TCATCCAGAA GTGGGAGGAG 
AAGTCCCGAG AGTTCATTGG AAGTTTCCTG GAAATGTTTG GTCCAGAAGG AGCGCTGAAG 
CACATGCTGA AAGAGGGAAA AGGTCGGATG CTGCAGGCCA TCAGTCCCAA GCAGAGTCCC 
AGCAGCAGCC CTACTCATGA GCGCTCCCCC TCCCCCTCCT TTCGGTGGCC CTTCTCTGGC 
AAGACTTCCC CATCTTCCTC CCCAGCAAGT CTCTCTAGGT GCAAGGCTGT GACCTGTGAC 
ATCAGCGAGG ATGAAGAGGA CTAA