Pdk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdk1
Entrez Gene ID	116551
Full Name	pyruvate dehydrogenase kinase 1
General protein information	Preferred Names pyruvate dehydrogenase kinase 1 Names pyruvate dehydrogenase kinase, isozyme 1 PDH kinase 1 PDK p48 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial pyruvate dehydrogenase kinase, isoenzyme 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q23
Summary	catalyzes the phosphorylation and inactivation of the pyruvate dehydrogenase complex [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053826.2	NP_446278.2	pyruvate dehydrogenase kinase, isozyme 1 precursor
XM_006234352.3	XP_006234414.1	pyruvate dehydrogenase kinase, isozyme 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDK1	NP_001265478.1
Bos taurus (cattle)	PDK1	NP_001192886.1
Mus musculus (house mouse)	Pdk1	NP_766253.2
Rattus norvegicus (Norway rat)	Pdk1	NP_446278.2
Gallus gallus (chicken)	PDK1	NP_001026523.2
Danio rerio (zebrafish)	pdk1	XP_683576.2
Canis lupus familiaris (dog)	PDK1	XP_534032.3
Xenopus tropicalis (tropical clawed frog)	pdk1	XP_002934664.2
Pan troglodytes (chimpanzee)	PDK1	XP_001147224.2

Gene Ontology
Bibliography

Related articles in PubMed

Activation of HIF-1α and its downstream targets in rat hippocampus after long-term simulated microgravity exposure.
Wang T, Chen H, Lv K, Ji G, Liang F, Zhang Y, Wang Y, Liu X, Cao H, Kan G, Xiong J, Li Y, Qu L
Biochemical and biophysical research communications485(3)591-597(2017 Apr)

Screening of differentially expressed protein kinases in bone marrow endothelial cells and the protective effects of the p38a inhibitor SB203580 on bone marrow in liver fibrosis.
Gao B, Sun W, Meng X, Xue D, Zhang W
Molecular medicine reports14(5)4629-4637(2016 Nov)

Elucidation of the interaction loci of the human pyruvate dehydrogenase complex E2·E3BP core with pyruvate dehydrogenase kinase 1 and kinase 2 by H/D exchange mass spectrometry and nuclear magnetic resonance.
Wang J, Kumaran S, Zhou J, Nemeria NS, Tao H, Kakalis L, Park YH, Birkaya B, Patel MS, Jordan F
Biochemistry54(1)69-82(2015 Jan)

Complex regulation of PKCβ2 and PDK-1/AKT by ROCK2 in diabetic heart.
Lin G, Brownsey RW, Macleod KM
PloS one9(1)e86520(2014)

Liver epithelial cells proliferate under hypoxia and protect the liver from ischemic injury via expression of HIF-1 alpha target genes.
Abe Y, Uchinami H, Kudoh K, Nakagawa Y, Ise N, Watanabe G, Sato T, Seki E, Yamamoto Y
Surgery152(5)869-78(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pdk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12425	NM_053826.2 Latest version!	Rattus norvegicus pyruvate dehydrogenase kinase 1 (Pdk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa25960	XM_006234352.3 Latest version!	Rattus norvegicus pyruvate dehydrogenase kinase 1 (Pdk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdk1 ( NM_053826.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446278.2 Rattus norvegicus pyruvate dehydrogenase kinase 1 (Pdk1), mRNA.

Price & Availability↑

CloneID	ORa12425
Clone ID Related Accession (Same CDS sequence)	NM_053826.2
Accession Version	NM_053826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564675200000
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate dehydrogenase kinase, isozyme 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089783.1. On or before Jun 25, 2006 this sequence version replaced XM_001059645.1, XM_001059583.1, NM_053826.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089783.1, L22294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGGCTGG CAAGGCTGCT GCGGGGCGGC ACGAGCGTCA GGCCGCTCTG TGCCGTCCCC 
TGCGCCAGCC GTAGTCTGGC CTCGGACTCG GCCTCGGGCT CCGGGCCGGC GTCGGAGAGC 
GGCGTTCCGG GCCAGGTGGA CTTCTATGCG CGCTTCTCGC CGTCGCCACT CTCCATGAAG 
CAGTTCCTGG ACTTCGGGTC AGTAAATGCT TGTGAAAAGA CCTCGTTCAT GTTTCTTCGA 
CAAGAGTTGC CTGTTAGATT GGCAAATATA ATGAAAGAAA TAAGTCTCCT TCCCGATAAT 
CTTCTCAGGA CCCCATCCGT ACAGCTGGTA CAAAGTTGGT ATATCCAAAG CCTTCAGGAG 
CTGCTTGACT TTAAGGACAA GAGTGCTGAG GATGCTAAAA CTATTTATGA ATTCACAGAC 
ACAGTGATAA GGATCAGAAA CCGGCACAAT GATGTCATCC CCACCATGGC CCAGGGTGTG 
ACTGAATACA AGGAGAGCTT CGGGGTGGAT CCTGTCACCA GCCAAAATGT TCAGTACTTT 
TTGGATCGGT TCTACATGAG TCGCATCTCA ATTAGAATGC TACTCAACCA GCACTCTTTA 
TTGTTTGGTG GAAAAGGAAG TCCATCTCAT CGAAAACACA TTGGAAGCAT AAATCCAAAC 
TGTGATGTAG TCGAGGTTAT TAAAGATGGC TATGAGAATG CGAGACGGCT TTGTGATTTG 
TATTACATTA ACTCTCCTGA ACTAGAACTT GAAGAACTAA ATGCGAAATC ACCAGGACAG 
CCAATACAAG TGGTTTATGT TCCGTCCCAT CTCTATCACA TGGTGTTTGA ACTGTTCAAG 
AACGCAATGA GGGCCACCAT GGAGCACCAT GCAGACAAAG GCGTTTATCC CCCGATTCAA 
GTTCACGTCA CACTGGGGGA GGAGGATTTG ACTGTGAAGA TGAGTGACCG GGGAGGTGGT 
GTTCCGCTGA GGAAGATCGA CAGACTCTTC AACTACATGT ACTCAACTGC ACCCCGGCCT 
CGTGTTGAGA CGTCCCGTGC GGTGCCCCTG GCTGGATTTG GTTACGGATT GCCCATATCA 
CGCCTCTATG CACAGTACTT CCAGGGAGAC CTAAAGCTGT ATTCCTTAGA GGGCTACGGG 
ACGGATGCTG TCATCTATAT TAAGGCTCTG TCAACGGAGT CCATCGAAAG ACTCCCTGTG 
TATAATAAAG CTGCCTGGAA GCATTACAGA ACCAACCACG AGGCTGACGA CTGGTGTGTG 
CCCAGCAGAG AGCCGAAAGA CATGACGACG TTCCGAAGCT CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446278.2
CDS	105..1409
Translation	MRLARLLRGGTSVRPLCAVPCASRSLASDSASGSGPASESGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESIERLPVYNKAAWKHYRTNHEADDWCVPSREPKDMTTFRSS

Target ORF information:

RefSeq Version	NM_053826.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate dehydrogenase kinase 1 (Pdk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053826.2

ORF Insert Sequence:

ATGAGGCTGG CAAGGCTGCT GCGGGGCGGC ACGAGCGTCA GGCCGCTCTG TGCCGTCCCC 
TGCGCCAGCC GTAGTCTGGC CTCGGACTCG GCCTCGGGCT CCGGGCCGGC GTCGGAGAGC 
GGCGTTCCGG GCCAGGTGGA CTTCTATGCG CGCTTCTCGC CGTCGCCACT CTCCATGAAG 
CAGTTCCTGG ACTTCGGGTC AGTAAATGCT TGTGAAAAGA CCTCGTTCAT GTTTCTTCGA 
CAAGAGTTGC CTGTTAGATT GGCAAATATA ATGAAAGAAA TAAGTCTCCT TCCCGATAAT 
CTTCTCAGGA CCCCATCCGT ACAGCTGGTA CAAAGTTGGT ATATCCAAAG CCTTCAGGAG 
CTGCTTGACT TTAAGGACAA GAGTGCTGAG GATGCTAAAA CTATTTATGA ATTCACAGAC 
ACAGTGATAA GGATCAGAAA CCGGCACAAT GATGTCATCC CCACCATGGC CCAGGGTGTG 
ACTGAATACA AGGAGAGCTT CGGGGTGGAT CCTGTCACCA GCCAAAATGT TCAGTACTTT 
TTGGATCGGT TCTACATGAG TCGCATCTCA ATTAGAATGC TACTCAACCA GCACTCTTTA 
TTGTTTGGTG GAAAAGGAAG TCCATCTCAT CGAAAACACA TTGGAAGCAT AAATCCAAAC 
TGTGATGTAG TCGAGGTTAT TAAAGATGGC TATGAGAATG CGAGACGGCT TTGTGATTTG 
TATTACATTA ACTCTCCTGA ACTAGAACTT GAAGAACTAA ATGCGAAATC ACCAGGACAG 
CCAATACAAG TGGTTTATGT TCCGTCCCAT CTCTATCACA TGGTGTTTGA ACTGTTCAAG 
AACGCAATGA GGGCCACCAT GGAGCACCAT GCAGACAAAG GCGTTTATCC CCCGATTCAA 
GTTCACGTCA CACTGGGGGA GGAGGATTTG ACTGTGAAGA TGAGTGACCG GGGAGGTGGT 
GTTCCGCTGA GGAAGATCGA CAGACTCTTC AACTACATGT ACTCAACTGC ACCCCGGCCT 
CGTGTTGAGA CGTCCCGTGC GGTGCCCCTG GCTGGATTTG GTTACGGATT GCCCATATCA 
CGCCTCTATG CACAGTACTT CCAGGGAGAC CTAAAGCTGT ATTCCTTAGA GGGCTACGGG 
ACGGATGCTG TCATCTATAT TAAGGCTCTG TCAACGGAGT CCATCGAAAG ACTCCCTGTG 
TATAATAAAG CTGCCTGGAA GCATTACAGA ACCAACCACG AGGCTGACGA CTGGTGTGTG 
CCCAGCAGAG AGCCGAAAGA CATGACGACG TTCCGAAGCT CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdk1 ( XM_006234352.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234414.1 Rattus norvegicus pyruvate dehydrogenase kinase 1 (Pdk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25960
Clone ID Related Accession (Same CDS sequence)	XM_006234352.3
Accession Version	XM_006234352.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate dehydrogenase kinase, isozyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTTC GACAAGAGTT GCCTGTTAGA TTGGCAAATA TAATGAAAGA AATAAGTCTC 
CTTCCCGATA ATCTTCTCAG GACCCCATCC GTACAGCTGG TACAAAGTTG GTATATCCAA 
AGCCTTCAGG AGCTGCTTGA CTTTAAGGAC AAGAGTGCTG AGGATGCTAA AACTATTTAT 
GAATTCACAG ACACAGTGAT AAGGATCAGA AACCGGCACA ATGATGTCAT CCCCACCATG 
GCCCAGGGTG TGACTGAATA CAAGGAGAGC TTCGGGGTGG ATCCTGTCAC CAGCCAAAAT 
GTTCAGTACT TTTTGGATCG GTTCTACATG AGTCGCATCT CAATTAGAAT GCTACTCAAC 
CAGCACTCTT TATTGTTTGG TGGAAAAGGA AGTCCATCTC ATCGAAAACA CATTGGAAGC 
ATAAATCCAA ACTGTGATGT AGTCGAGGTT ATTAAAGATG GCTATGAGAA TGCGAGACGG 
CTTTGTGATT TGTATTACAT TAACTCTCCT GAACTAGAAC TTGAAGAACT AAATGCGAAA 
TCACCAGGAC AGCCAATACA AGTGGTTTAT GTTCCGTCCC ATCTCTATCA CATGGTGTTT 
GAACTGTTCA AGAACGCAAT GAGGGCCACC ATGGAGCACC ATGCAGACAA AGGCGTTTAT 
CCCCCGATTC AAGTTCACGT CACACTGGGG GAGGAGGATT TGACTGTGAA GATGAGTGAC 
CGGGGAGGTG GTGTTCCGCT GAGGAAGATC GACAGACTCT TCAACTACAT GTACTCAACT 
GCACCCCGGC CTCGTGTTGA GACGTCCCGT GCGGTGCCCC TGGCTGGATT TGGTTACGGA 
TTGCCCATAT CACGCCTCTA TGCACAGTAC TTCCAGGGAG ACCTAAAGCT GTATTCCTTA 
GAGGGCTACG GGACGGATGC TGTCATCTAT ATTAAGGCTC TGTCAACGGA GTCCATCGAA 
AGACTCCCTG TGTATAATAA AGCTGCCTGG AAGCATTACA GAACCAACCA CGAGGCTGAC 
GACTGGTGTG TGCCCAGCAG AGAGCCGAAA GACATGACGA CGTTCCGAAG CTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234414.1
CDS	106..1182
Translation	MFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESIERLPVYNKAAWKHYRTNHEADDWCVPSREPKDMTTFRSS

Target ORF information:

RefSeq Version	XM_006234352.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate dehydrogenase kinase 1 (Pdk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234352.3

ORF Insert Sequence:

ATGTTTCTTC GACAAGAGTT GCCTGTTAGA TTGGCAAATA TAATGAAAGA AATAAGTCTC 
CTTCCCGATA ATCTTCTCAG GACCCCATCC GTACAGCTGG TACAAAGTTG GTATATCCAA 
AGCCTTCAGG AGCTGCTTGA CTTTAAGGAC AAGAGTGCTG AGGATGCTAA AACTATTTAT 
GAATTCACAG ACACAGTGAT AAGGATCAGA AACCGGCACA ATGATGTCAT CCCCACCATG 
GCCCAGGGTG TGACTGAATA CAAGGAGAGC TTCGGGGTGG ATCCTGTCAC CAGCCAAAAT 
GTTCAGTACT TTTTGGATCG GTTCTACATG AGTCGCATCT CAATTAGAAT GCTACTCAAC 
CAGCACTCTT TATTGTTTGG TGGAAAAGGA AGTCCATCTC ATCGAAAACA CATTGGAAGC 
ATAAATCCAA ACTGTGATGT AGTCGAGGTT ATTAAAGATG GCTATGAGAA TGCGAGACGG 
CTTTGTGATT TGTATTACAT TAACTCTCCT GAACTAGAAC TTGAAGAACT AAATGCGAAA 
TCACCAGGAC AGCCAATACA AGTGGTTTAT GTTCCGTCCC ATCTCTATCA CATGGTGTTT 
GAACTGTTCA AGAACGCAAT GAGGGCCACC ATGGAGCACC ATGCAGACAA AGGCGTTTAT 
CCCCCGATTC AAGTTCACGT CACACTGGGG GAGGAGGATT TGACTGTGAA GATGAGTGAC 
CGGGGAGGTG GTGTTCCGCT GAGGAAGATC GACAGACTCT TCAACTACAT GTACTCAACT 
GCACCCCGGC CTCGTGTTGA GACGTCCCGT GCGGTGCCCC TGGCTGGATT TGGTTACGGA 
TTGCCCATAT CACGCCTCTA TGCACAGTAC TTCCAGGGAG ACCTAAAGCT GTATTCCTTA 
GAGGGCTACG GGACGGATGC TGTCATCTAT ATTAAGGCTC TGTCAACGGA GTCCATCGAA 
AGACTCCCTG TGTATAATAA AGCTGCCTGG AAGCATTACA GAACCAACCA CGAGGCTGAC 
GACTGGTGTG TGCCCAGCAG AGAGCCGAAA GACATGACGA CGTTCCGAAG CTCGTAA