Dgkq cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgkq
Entrez Gene ID	100361138
Full Name	diacylglycerol kinase, theta
General protein information	Preferred Names diacylglycerol kinase, theta Names diacylglycerol kinase theta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001198804.1	NP_001185733.1	diacylglycerol kinase theta

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DGKQ	NP_001193234.1
Gallus gallus (chicken)	DGKQ	XP_424953.3
Pan troglodytes (chimpanzee)	DGKQ	XP_517054.3
Mus musculus (house mouse)	Dgkq	NP_950176.1
Rattus norvegicus (Norway rat)	Dgkq	NP_001185733.1
Drosophila melanogaster (fruit fly)	CG31140	NP_996276.1
Caenorhabditis elegans (roundworm)	dgk-1	NP_508190.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004209	XP_313113.4
Homo sapiens (human)	DGKQ	NP_001338.2
Canis lupus familiaris (dog)	DGKQ	XP_005618812.1
Xenopus tropicalis (tropical clawed frog)	dgkq	XP_002935917.1

Gene Ontology
Bibliography

Related articles in PubMed

DGKθ Catalytic Activity Is Required for Efficient Recycling of Presynaptic Vesicles at Excitatory Synapses.
Goldschmidt HL, Tu-Sekine B, Volk L, Anggono V, Huganir RL, Raben DM
Cell reports14(2)200-7(2016 Jan)

Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells.
Cai K, Lucki NC, Sewer MB
Biochimica et biophysica acta1841(4)552-62(2014 Apr)

Diacylglycerol kinase θ couples farnesoid X receptor-dependent bile acid signalling to Akt activation and glucose homoeostasis in hepatocytes.
Cai K, Sewer MB
The Biochemical journal454(2)267-74(2013 Sep)

cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1.
Cai K, Sewer MB
Journal of lipid research54(8)2121-32(2013 Aug)

Dual regulation of diacylglycerol kinase (DGK)-θ: polybasic proteins promote activation by phospholipids and increase substrate affinity.
Tu-Sekine B, Raben DM
The Journal of biological chemistry287(50)41619-27(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dgkq gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgkq cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08597	NM_001198804.1 Latest version!	Rattus norvegicus diacylglycerol kinase, theta (Dgkq), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgkq ( NM_001198804.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001185733.1 Rattus norvegicus diacylglycerol kinase, theta (Dgkq), mRNA.

Price & Availability↑

CloneID	ORa08597
Clone ID Related Accession (Same CDS sequence)	NM_001198804.1
Accession Version	NM_001198804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase theta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117047.5. On Nov 11, 2010 this sequence version replaced XM_002728069.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGG CAGCCGAGTC CGGGGCCCGG ACCTGGCCGG GCAGTGGTTC CCCACGTCTC 
GGCAGCCCGG CCGGCAGCCC TGTGCTGGGC ATCTCGGGCC GCGCACGCCC GGGGTCAGGG 
CCGGAGCGGA CTGGCAGAGC TATCGGCTCC GTAGCACCCG GCCACAGCTT TCGCAAGGTG 
ACACTCACCA AGCCTACCTT CTGCCACCTC TGCTCGGACT TCATCTGGGG ATTGGCCGGC 
TTCCTGTGCG ATGTCTGCAA CTTCATGTCC CATGAGAAGT GCCTAAAGCA GGTGAAGACC 
CCGTGCACAA GCATTGCGCC AAGCCTCGTC CGGGTCCCTG TAGCCCACTG CTTTGGTTCC 
CTTGGTCTCT ACAAGCGCAA GTTCTGTGTG GTCTGCCGCA AGAGCCTGGA GGTACCTGCG 
TTCCGCTGTG AAGTGTGTGA GCTGCACGTT CACCCCGACT GTGTGCCCTT CGCCTGCAGC 
GACTGTCGTC AGTGTCACCA GGATGGACAG CACGATTATG ACACGTATCA CCACCACTGG 
AGGGAGGGGA ATCTGCCTTC TGGTGCTCGA TGTGAGGTCT GTAGGAAGAC TTGTGGCTCC 
TCGGATGTGC TGGCTGGTGT ACGCTGCGAG TGGTGTGGTG TACAGGCTCA CTCCGTGTGC 
TCCACAGCAC TCACCCCTGA GTGTACATTT GGACGTCTAC GCTCCATGGT ACTGCCTCCT 
TCGTGTGTGC GCCTGTTGTC CCGAAACTTC AGCAAGATGC ACTGCTTCCG AATCCCCGAG 
ACCATGGTCC TGGAGCTCGG TGATGGGGAT GATGGCCTAG ATGGGAGTGC TGCAGTCGGC 
ACAGGCAGAG AGGTATCGGC AGCTACAGAA TCTACCAAAC AGACCCTGAA GATCTTTGAT 
GGCAACGACT CCATGAGGAA AAATCAGTTT CGCCTGGTTA CAGTTTCCCG CCTAGCTCGG 
AATGAGGAAG TGATGGAGGC AGCACTCCGG GCCTACTATA TCAACGAGGA CCCTAAGGAC 
TTCCAGCTGC AGGCACTGCC CCTAACGTTA CTGTCTGGCA ATGCCCAGGC TCTGGGGAAG 
GCCGGGACCA CTGAGGAGGA GACAAGTAAA GACTCCGGGC CCGGGGATTC CGTGCCTGAG 
GCCTGGGTCA TCAGGTCTCT GCCTCGTACC CAAGAGATCC TGAAGATCTA CCCTGACTGG 
CTCAAGGTAG GTGTGGCCTA CGTGTCCATC CGTGTGAACT CCCAGAGTAC AGCACGGTCT 
GTGGTTCAAG AGGTTCTCCC ACTGTTTGGA CGACAGGTTG AGGATCAGGA GAGATTCCAG 
CTGATTGAGG TGCTTATGAG CAGCAGACAA GTCCAACGGA CGGTGCTGGT GGATGAAGAA 
CCTCTGCTAG ATCGACTCCG GGACATCCGA CAGACATCTG TGCGCCAGGC GAGCCAGACG 
CGGTTCTATG TGGCCGAGGC CAGGGCCGTG ACCCCACATG TCTCCCTGTT TGTGGGTGGC 
CTGCCACCTG GCTTGTCCCC TCAGGATTAC AGCAACCTGC TGCATGAGGC CATGGCCACC 
AAAGCTGCTG TGGTGTCTGT GAGTCACGTC TACTCCTTAC AAGGTGCGGT AGTTCTGGAT 
GTCACCTGCT TCGCGGAGGC TGAGCGGCTA TACATGCTGG CCAGGGACAC AGCAGTGCAT 
GGCCGGCCAC TGACTGCACT AGTGCTTCCA GATGTGCTGC ACACGAAGCT GCCTCCTGAC 
TGCTGCCCTC TCCTCGTGTT TGTGAACCCC AAGAGTGGGG GCCTCAAGGG ACGAGAACTG 
CTCTGCAGTT TCCGGAAGCT GCTGAATCCA CACCAGGTCT TTGAGCTCAC CAACGGGGGC 
CCTCTTCCTG GGTTCCACCT TTTCTCCCAG GTGCCCTGCT TTCGGGTACT GGTCTGTGGT 
GGAGATGGCA CCGTGGGCTG GGTGCTCGCT GCCCTGGAGG AGACAAGGCG CCATCTGGCC 
TGCCCAGAGC CATCTGTGGC CATCCTACCC CTGGGTACAG GGAATGACCT TGGCCGGGTC 
CTCCGTTGGG GAGCAGGCTA TAGTGGTGAG GACCCATTTT CTGTGCTGGT GTCTGTGGAT 
GAGGCTGATG CTGTGCTCAT GGATCGATGG ACAATCCTGC TGGACGCTCA TGAAATTGAT 
AGTACAGAGA ACAATGTGGT AGAAACAGAG CCCCCCAAGA TTGTTCAGAT GAATAACTAC 
TGTGGCATTG GCATTGATGC GGAGCTCAGC CTAGACTTCC ACCAGGCACG TGAAGAGGAG 
CCTGGCAAAT TCACAAGCAG GTTCCACAAC AAGGGCGTGT ATGTGCGGGT TGGGCTGCAG 
AAGATCAGCC ACTCTCGAAG CCTGCACAAG GAGATCCGTC TGCAGGTGGA GCAGCAGGAA 
GTGGAGCTGC CCAGCATTGA GGGTCTTATC TTCATTAACA TCCCCAGCTG GGGCTCAGGG 
GCTGACCTGT GGGGCTCTGA CAGTGACTCA AGGTTTGAGA AGCCACGCAT AGACGACGGG 
CTGTTGGAGG TGGTGGGTGT GACAGGTGTC GTGCACATGG GCCAGGTACA AGGTGGGCTG 
CGCTCTGGAA TCCGAATCGC CCAGGGCTCC TACTTCCGTG TCACACTCCT CAAGGCTACT 
CCAGTGCAGG TGGACGGTGA GCCCTGGATT CAGGCCCCAG GTCACATGAT CATCTCTGCT 
ACTGCACCTA AGGTTCACAT GCTGAGGAAA GCTAAGCAGA AGCCCAGGAA GGCTGGCGCC 
ATCAGGGATA CCCGAGTGGA CACCTTGCCT GCTCCTGAGG GCAATCCTTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185733.1
CDS	88..2901
Translation	MATAAESGARTWPGSGSPRLGSPAGSPVLGISGRARPGSGPERTGRAIGSVAPGHSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKQVKTPCTSIAPSLVRVPVAHCFGSLGLYKRKFCVVCRKSLEVPAFRCEVCELHVHPDCVPFACSDCRQCHQDGQHDYDTYHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSVCSTALTPECTFGRLRSMVLPPSCVRLLSRNFSKMHCFRIPETMVLELGDGDDGLDGSAAVGTGREVSAATESTKQTLKIFDGNDSMRKNQFRLVTVSRLARNEEVMEAALRAYYINEDPKDFQLQALPLTLLSGNAQALGKAGTTEEETSKDSGPGDSVPEAWVIRSLPRTQEILKIYPDWLKVGVAYVSIRVNSQSTARSVVQEVLPLFGRQVEDQERFQLIEVLMSSRQVQRTVLVDEEPLLDRLRDIRQTSVRQASQTRFYVAEARAVTPHVSLFVGGLPPGLSPQDYSNLLHEAMATKAAVVSVSHVYSLQGAVVLDVTCFAEAERLYMLARDTAVHGRPLTALVLPDVLHTKLPPDCCPLLVFVNPKSGGLKGRELLCSFRKLLNPHQVFELTNGGPLPGFHLFSQVPCFRVLVCGGDGTVGWVLAALEETRRHLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLVSVDEADAVLMDRWTILLDAHEIDSTENNVVETEPPKIVQMNNYCGIGIDAELSLDFHQAREEEPGKFTSRFHNKGVYVRVGLQKISHSRSLHKEIRLQVEQQEVELPSIEGLIFINIPSWGSGADLWGSDSDSRFEKPRIDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWIQAPGHMIISATAPKVHMLRKAKQKPRKAGAIRDTRVDTLPAPEGNPL

Target ORF information:

RefSeq Version	NM_001198804.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, theta (Dgkq), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198804.1

ORF Insert Sequence:

ATGGCGACGG CAGCCGAGTC CGGGGCCCGG ACCTGGCCGG GCAGTGGTTC CCCACGTCTC 
GGCAGCCCGG CCGGCAGCCC TGTGCTGGGC ATCTCGGGCC GCGCACGCCC GGGGTCAGGG 
CCGGAGCGGA CTGGCAGAGC TATCGGCTCC GTAGCACCCG GCCACAGCTT TCGCAAGGTG 
ACACTCACCA AGCCTACCTT CTGCCACCTC TGCTCGGACT TCATCTGGGG ATTGGCCGGC 
TTCCTGTGCG ATGTCTGCAA CTTCATGTCC CATGAGAAGT GCCTAAAGCA GGTGAAGACC 
CCGTGCACAA GCATTGCGCC AAGCCTCGTC CGGGTCCCTG TAGCCCACTG CTTTGGTTCC 
CTTGGTCTCT ACAAGCGCAA GTTCTGTGTG GTCTGCCGCA AGAGCCTGGA GGTACCTGCG 
TTCCGCTGTG AAGTGTGTGA GCTGCACGTT CACCCCGACT GTGTGCCCTT CGCCTGCAGC 
GACTGTCGTC AGTGTCACCA GGATGGACAG CACGATTATG ACACGTATCA CCACCACTGG 
AGGGAGGGGA ATCTGCCTTC TGGTGCTCGA TGTGAGGTCT GTAGGAAGAC TTGTGGCTCC 
TCGGATGTGC TGGCTGGTGT ACGCTGCGAG TGGTGTGGTG TACAGGCTCA CTCCGTGTGC 
TCCACAGCAC TCACCCCTGA GTGTACATTT GGACGTCTAC GCTCCATGGT ACTGCCTCCT 
TCGTGTGTGC GCCTGTTGTC CCGAAACTTC AGCAAGATGC ACTGCTTCCG AATCCCCGAG 
ACCATGGTCC TGGAGCTCGG TGATGGGGAT GATGGCCTAG ATGGGAGTGC TGCAGTCGGC 
ACAGGCAGAG AGGTATCGGC AGCTACAGAA TCTACCAAAC AGACCCTGAA GATCTTTGAT 
GGCAACGACT CCATGAGGAA AAATCAGTTT CGCCTGGTTA CAGTTTCCCG CCTAGCTCGG 
AATGAGGAAG TGATGGAGGC AGCACTCCGG GCCTACTATA TCAACGAGGA CCCTAAGGAC 
TTCCAGCTGC AGGCACTGCC CCTAACGTTA CTGTCTGGCA ATGCCCAGGC TCTGGGGAAG 
GCCGGGACCA CTGAGGAGGA GACAAGTAAA GACTCCGGGC CCGGGGATTC CGTGCCTGAG 
GCCTGGGTCA TCAGGTCTCT GCCTCGTACC CAAGAGATCC TGAAGATCTA CCCTGACTGG 
CTCAAGGTAG GTGTGGCCTA CGTGTCCATC CGTGTGAACT CCCAGAGTAC AGCACGGTCT 
GTGGTTCAAG AGGTTCTCCC ACTGTTTGGA CGACAGGTTG AGGATCAGGA GAGATTCCAG 
CTGATTGAGG TGCTTATGAG CAGCAGACAA GTCCAACGGA CGGTGCTGGT GGATGAAGAA 
CCTCTGCTAG ATCGACTCCG GGACATCCGA CAGACATCTG TGCGCCAGGC GAGCCAGACG 
CGGTTCTATG TGGCCGAGGC CAGGGCCGTG ACCCCACATG TCTCCCTGTT TGTGGGTGGC 
CTGCCACCTG GCTTGTCCCC TCAGGATTAC AGCAACCTGC TGCATGAGGC CATGGCCACC 
AAAGCTGCTG TGGTGTCTGT GAGTCACGTC TACTCCTTAC AAGGTGCGGT AGTTCTGGAT 
GTCACCTGCT TCGCGGAGGC TGAGCGGCTA TACATGCTGG CCAGGGACAC AGCAGTGCAT 
GGCCGGCCAC TGACTGCACT AGTGCTTCCA GATGTGCTGC ACACGAAGCT GCCTCCTGAC 
TGCTGCCCTC TCCTCGTGTT TGTGAACCCC AAGAGTGGGG GCCTCAAGGG ACGAGAACTG 
CTCTGCAGTT TCCGGAAGCT GCTGAATCCA CACCAGGTCT TTGAGCTCAC CAACGGGGGC 
CCTCTTCCTG GGTTCCACCT TTTCTCCCAG GTGCCCTGCT TTCGGGTACT GGTCTGTGGT 
GGAGATGGCA CCGTGGGCTG GGTGCTCGCT GCCCTGGAGG AGACAAGGCG CCATCTGGCC 
TGCCCAGAGC CATCTGTGGC CATCCTACCC CTGGGTACAG GGAATGACCT TGGCCGGGTC 
CTCCGTTGGG GAGCAGGCTA TAGTGGTGAG GACCCATTTT CTGTGCTGGT GTCTGTGGAT 
GAGGCTGATG CTGTGCTCAT GGATCGATGG ACAATCCTGC TGGACGCTCA TGAAATTGAT 
AGTACAGAGA ACAATGTGGT AGAAACAGAG CCCCCCAAGA TTGTTCAGAT GAATAACTAC 
TGTGGCATTG GCATTGATGC GGAGCTCAGC CTAGACTTCC ACCAGGCACG TGAAGAGGAG 
CCTGGCAAAT TCACAAGCAG GTTCCACAAC AAGGGCGTGT ATGTGCGGGT TGGGCTGCAG 
AAGATCAGCC ACTCTCGAAG CCTGCACAAG GAGATCCGTC TGCAGGTGGA GCAGCAGGAA 
GTGGAGCTGC CCAGCATTGA GGGTCTTATC TTCATTAACA TCCCCAGCTG GGGCTCAGGG 
GCTGACCTGT GGGGCTCTGA CAGTGACTCA AGGTTTGAGA AGCCACGCAT AGACGACGGG 
CTGTTGGAGG TGGTGGGTGT GACAGGTGTC GTGCACATGG GCCAGGTACA AGGTGGGCTG 
CGCTCTGGAA TCCGAATCGC CCAGGGCTCC TACTTCCGTG TCACACTCCT CAAGGCTACT 
CCAGTGCAGG TGGACGGTGA GCCCTGGATT CAGGCCCCAG GTCACATGAT CATCTCTGCT 
ACTGCACCTA AGGTTCACAT GCTGAGGAAA GCTAAGCAGA AGCCCAGGAA GGCTGGCGCC 
ATCAGGGATA CCCGAGTGGA CACCTTGCCT GCTCCTGAGG GCAATCCTTT ATAG