Spns2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spns2
Entrez Gene ID	100270678
Full Name	sphingolipid transporter 2
General protein information	Preferred Names sphingolipid transporter 2 Names protein spinster homolog 2 spinster homolog 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144991.1	NP_001138463.1	protein spinster homolog 2
XM_006246563.3	XP_006246625.2	protein spinster homolog 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPNS2	XP_005620371.1
Danio rerio (zebrafish)	spns2	NP_001077316.1
Mus musculus (house mouse)	Spns2	NP_694700.2
Macaca mulatta (Rhesus monkey)	LOC721549	XP_001117725.2
Gallus gallus (chicken)	SPNS2	XP_004946501.1
Homo sapiens (human)	SPNS2	NP_001118230.1
Bos taurus (cattle)	SPNS2	NP_001193083.1
Xenopus tropicalis (tropical clawed frog)	spns2	NP_001120149.1
Rattus norvegicus (Norway rat)	Spns2	NP_001138463.1

Gene Ontology
Bibliography

Related articles in PubMed

Spns2, a transporter of phosphorylated sphingoid bases, regulates their blood and lymph levels, and the lymphatic network.
Nagahashi M, Kim EY, Yamada A, Ramachandran S, Allegood JC, Hait NC, Maceyka M, Milstien S, Takabe K, Spiegel S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(3)1001-11(2013 Mar)

The role of sphingosine-1-phosphate transporter Spns2 in immune system function.
Nijnik A, Clare S, Hale C, Chen J, Raisen C, Mottram L, Lucas M, Estabel J, Ryder E, Adissu H, , Adams NC, Ramirez-Solis R, White JK, Steel KP, Dougan G, Hancock RE
Journal of immunology (Baltimore, Md. : 1950)189(1)102-11(2012 Jul)

The sphingosine-1-phosphate transporter Spns2 expressed on endothelial cells regulates lymphocyte trafficking in mice.
Fukuhara S, Simmons S, Kawamura S, Inoue A, Orba Y, Tokudome T, Sunden Y, Arai Y, Moriwaki K, Ishida J, Uemura A, Kiyonari H, Abe T, Fukamizu A, Hirashima M, Sawa H, Aoki J, Ishii M, Mochizuki N
The Journal of clinical investigation122(4)1416-26(2012 Apr)

Rapid-throughput skeletal phenotyping of 100 knockout mice identifies 9 new genes that determine bone strength.
Bassett JH, Gogakos A, White JK, Evans H, Jacques RM, van der Spek AH, , Ramirez-Solis R, Ryder E, Sunter D, Boyde A, Campbell MJ, Croucher PI, Williams GR
PLoS genetics8(8)e1002858(2012)

The sphingosine 1-phosphate transporter, SPNS2, functions as a transporter of the phosphorylated form of the immunomodulating agent FTY720.
Hisano Y, Kobayashi N, Kawahara A, Yamaguchi A, Nishi T
The Journal of biological chemistry286(3)1758-66(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spns2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spns2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04460	NM_001144991.1 Latest version!	Rattus norvegicus sphingolipid transporter 2 (Spns2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa50674	XM_006246563.3 Latest version!	Rattus norvegicus spinster homolog 2 (Spns2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spns2 ( NM_001144991.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001138463.1 Rattus norvegicus sphingolipid transporter 2 (Spns2), mRNA.

Price & Availability↑

CloneID	ORa04460
Clone ID Related Accession (Same CDS sequence)	NM_001144991.1
Accession Version	NM_001144991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	protein spinster homolog 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATGTGCC TGGAATGCGC CTCGGCGGCG GCGGGCGGCG CGGAGGAGGA GGAGGCGGAC 
GCCGAACGGC GGCGCCGGCG CCGGGGGGCG CAGCCAGGGG CTGGAGGTAG CGCTTGCTGC 
GGGGCGCGGG GTGTTGGGGG CGCTGGAGTC GTGTCCGCGG ACGAAGAGGT GCAGACGTTG 
TCCGGCAGCG TGAGGAGGGT CCCGTCCGGA CTCCCCAGCA TCCCCAGTAC CCCGGGCTGC 
GCAGCCGCTG CCAAGGGCCC CGGCGCGCCG CAGCCCAAAC CCGCCAGCTT GGGCCGAGGG 
CGGGGGGCAG CCGCTGCCAT CCTGAGCTTG GGCAACGTGC TCAACTACCT GGACAGGTAC 
ACTGTGGCAG GTGTTCTTCT GGACATCCAG CAGCACTTTG GGGTCAAGGA CCGGGGCGCT 
GGCTTGCTGC AGTCAGTGTT CATCTGCAGC TTCATGGTGG CTGCCCCCAT CTTTGGCTAC 
CTGGGCGATC GCTTCAATAG AAAGGTGATC CTCAGCTGCG GCATCTTCTT CTGGTCGGCC 
GTCACCTTCT CTAGTTCTTT CATCCCTCAG CAGTACTTCT GGCTGCTGGT CCTGTCCCGG 
GGGCTAGTAG GTATTGGTGA AGCTAGCTAT TCCACCATAG CACCCACCAT CATCGGCGAC 
TTATTTACCA AGAACACACG CACACTGATG CTATCAGTCT TCTATTTTGC CATCCCTCTG 
GGCAGTGGCC TGGGCTACAT CACAGGTTCC AGCGTGAAGC AGGCAGCTGG AGACTGGCAT 
TGGGCCCTGC GGGTTTCCCC TGTCCTGGGC ATGATCACAG GAACACTCAT CCTCATCTTG 
GTCCCAGCCA CTAAGAGAGG CCATGCTGAT CAACTTGGGG GGCAGCTCAA AGCACGGACC 
TCCTGGCTCC GAGACATGAA GGCCCTGATC CGGAACCGCA GTTACGTCTT CTCCTCCCTG 
GCCACATCCG CTGTGTCCTT CGCCACAGGA GCCCTGGGCA TGTGGATTCC CCTCTATCTG 
CACCGTGCTC AAGTTGTACA AAAGACAGCA GAGACTTGCA ACAGCCCGCC CTGTGGAGCC 
AAAGACAGCC TCATCTTTGG GGCCATTACC TGCTTTACTG GCTTTCTGGG CGTAGTCACA 
GGCGCGGGAG CCACTCGCTG GTGCCGCTTG CGCACTCAGC GCGCTGACCC CTTGGTGTGC 
GCCGTGGGCA TGCTGGGATC TGCCATCTTC ATCTGCCTCA TCTTTGTGGC TGCCAAGACC 
AGCATCGTGG GCGCCTATAT CTGCATCTTT GTTGGCGAGA CACTGCTGTT TTCTAACTGG 
GCCATCACTG CAGACATCCT CATGTATGTG GTCATCCCCA CTCGACGAGC CACTGCTGTG 
GCCTTGCAGA GCTTCACCTC CCATCTGCTG GGGGACGCTG GAAGCCCCTA CCTCATTGGC 
TTTATCTCGG ACCTAATCCG CCAGAGCACC AAGGACTCCC CACTCTGGGA GTTTCTGAGC 
CTGGGCTATG CCCTCATGCT GTGCCCTTTT GTTGTGGTCC TGGGTGGCAT GTTCTTCCTT 
GCCACTGCTC TCTTCTTCCT CAGCGATCGT GCCAAGGCTG AGCAGCAGGC TGACGAGGTC 
CCTGCCCAGC GCCTCCGGTC TACCGGCCCC AGTGATTCGA TGTGCCAGAG CCGTGCCTGG 
GCCCAGTCCC CGCTGATGTG CCACCCTGAC CCCCGTCCGT CTCTCCCTCA GGGTGAACCA 
GCTGGTGATG CCGCCTGCAT CCGTGAAAAT CTGAGGTGGT GCCGCTGGGA CAGCAATGAA 
CTGCAGTTTA GATTGGGAGA GGTTCTCCAG CATCCTGGAC CTGCAGGACG GTCGCAAAGC 
TTCCTGTGTG ACTCACAGTT GGGTACACAC CCTCTTTGGC CTAGGACTGC CAGGACTGAG 
TGGCCCTATC TTCAAGAAGC TGCATCCTCA GTGAACCTAG AAGGCTGTGT GTGCTGGAGT 
CACTCAGTTG GACACAACTC CAGCCCTGGT TGGGGGCTGA AGGGCCAAGA TAGAAGCAGC 
CACCCTGCAG TGGGTATTTG GGGAGATTCT GGTCCACCCT TAGCTCATGT GATCTTGAGG 
GAGCCACTAT CCTCCCTAGA CAGTGGAACC AAGGAGCTGG AGCTTCCTCC ATTGTGTGCC 
ATGCATGGCA CCCTCCACAA CTTTGAAGAA ACAGGCCCGG GGCTGTACAC AGAAGACACC 
GCTCAGGTCT CAGGACCTCA GTTCCGGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138463.1
CDS	179..2431
Translation	MMCLECASAAAGGAEEEEADAERRRRRRGAQPGAGGSACCGARGVGGAGVVSADEEVQTLSGSVRRVPSGLPSIPSTPGCAAAAKGPGAPQPKPASLGRGRGAAAAILSLGNVLNYLDRYTVAGVLLDIQQHFGVKDRGAGLLQSVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSAVTFSSSFIPQQYFWLLVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQAAGDWHWALRVSPVLGMITGTLILILVPATKRGHADQLGGQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGMWIPLYLHRAQVVQKTAETCNSPPCGAKDSLIFGAITCFTGFLGVVTGAGATRWCRLRTQRADPLVCAVGMLGSAIFICLIFVAAKTSIVGAYICIFVGETLLFSNWAITADILMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIRQSTKDSPLWEFLSLGYALMLCPFVVVLGGMFFLATALFFLSDRAKAEQQADEVPAQRLRSTGPSDSMCQSRAWAQSPLMCHPDPRPSLPQGEPAGDAACIRENLRWCRWDSNELQFRLGEVLQHPGPAGRSQSFLCDSQLGTHPLWPRTARTEWPYLQEAASSVNLEGCVCWSHSVGHNSSPGWGLKGQDRSSHPAVGIWGDSGPPLAHVILREPLSSLDSGTKELELPPLCAMHGTLHNFEETGPGLYTEDTAQVSGPQFRF

Target ORF information:

RefSeq Version	NM_001144991.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingolipid transporter 2 (Spns2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144991.1

ORF Insert Sequence:

ATGATGTGCC TGGAATGCGC CTCGGCGGCG GCGGGCGGCG CGGAGGAGGA GGAGGCGGAC 
GCCGAACGGC GGCGCCGGCG CCGGGGGGCG CAGCCAGGGG CTGGAGGTAG CGCTTGCTGC 
GGGGCGCGGG GTGTTGGGGG CGCTGGAGTC GTGTCCGCGG ACGAAGAGGT GCAGACGTTG 
TCCGGCAGCG TGAGGAGGGT CCCGTCCGGA CTCCCCAGCA TCCCCAGTAC CCCGGGCTGC 
GCAGCCGCTG CCAAGGGCCC CGGCGCGCCG CAGCCCAAAC CCGCCAGCTT GGGCCGAGGG 
CGGGGGGCAG CCGCTGCCAT CCTGAGCTTG GGCAACGTGC TCAACTACCT GGACAGGTAC 
ACTGTGGCAG GTGTTCTTCT GGACATCCAG CAGCACTTTG GGGTCAAGGA CCGGGGCGCT 
GGCTTGCTGC AGTCAGTGTT CATCTGCAGC TTCATGGTGG CTGCCCCCAT CTTTGGCTAC 
CTGGGCGATC GCTTCAATAG AAAGGTGATC CTCAGCTGCG GCATCTTCTT CTGGTCGGCC 
GTCACCTTCT CTAGTTCTTT CATCCCTCAG CAGTACTTCT GGCTGCTGGT CCTGTCCCGG 
GGGCTAGTAG GTATTGGTGA AGCTAGCTAT TCCACCATAG CACCCACCAT CATCGGCGAC 
TTATTTACCA AGAACACACG CACACTGATG CTATCAGTCT TCTATTTTGC CATCCCTCTG 
GGCAGTGGCC TGGGCTACAT CACAGGTTCC AGCGTGAAGC AGGCAGCTGG AGACTGGCAT 
TGGGCCCTGC GGGTTTCCCC TGTCCTGGGC ATGATCACAG GAACACTCAT CCTCATCTTG 
GTCCCAGCCA CTAAGAGAGG CCATGCTGAT CAACTTGGGG GGCAGCTCAA AGCACGGACC 
TCCTGGCTCC GAGACATGAA GGCCCTGATC CGGAACCGCA GTTACGTCTT CTCCTCCCTG 
GCCACATCCG CTGTGTCCTT CGCCACAGGA GCCCTGGGCA TGTGGATTCC CCTCTATCTG 
CACCGTGCTC AAGTTGTACA AAAGACAGCA GAGACTTGCA ACAGCCCGCC CTGTGGAGCC 
AAAGACAGCC TCATCTTTGG GGCCATTACC TGCTTTACTG GCTTTCTGGG CGTAGTCACA 
GGCGCGGGAG CCACTCGCTG GTGCCGCTTG CGCACTCAGC GCGCTGACCC CTTGGTGTGC 
GCCGTGGGCA TGCTGGGATC TGCCATCTTC ATCTGCCTCA TCTTTGTGGC TGCCAAGACC 
AGCATCGTGG GCGCCTATAT CTGCATCTTT GTTGGCGAGA CACTGCTGTT TTCTAACTGG 
GCCATCACTG CAGACATCCT CATGTATGTG GTCATCCCCA CTCGACGAGC CACTGCTGTG 
GCCTTGCAGA GCTTCACCTC CCATCTGCTG GGGGACGCTG GAAGCCCCTA CCTCATTGGC 
TTTATCTCGG ACCTAATCCG CCAGAGCACC AAGGACTCCC CACTCTGGGA GTTTCTGAGC 
CTGGGCTATG CCCTCATGCT GTGCCCTTTT GTTGTGGTCC TGGGTGGCAT GTTCTTCCTT 
GCCACTGCTC TCTTCTTCCT CAGCGATCGT GCCAAGGCTG AGCAGCAGGC TGACGAGGTC 
CCTGCCCAGC GCCTCCGGTC TACCGGCCCC AGTGATTCGA TGTGCCAGAG CCGTGCCTGG 
GCCCAGTCCC CGCTGATGTG CCACCCTGAC CCCCGTCCGT CTCTCCCTCA GGGTGAACCA 
GCTGGTGATG CCGCCTGCAT CCGTGAAAAT CTGAGGTGGT GCCGCTGGGA CAGCAATGAA 
CTGCAGTTTA GATTGGGAGA GGTTCTCCAG CATCCTGGAC CTGCAGGACG GTCGCAAAGC 
TTCCTGTGTG ACTCACAGTT GGGTACACAC CCTCTTTGGC CTAGGACTGC CAGGACTGAG 
TGGCCCTATC TTCAAGAAGC TGCATCCTCA GTGAACCTAG AAGGCTGTGT GTGCTGGAGT 
CACTCAGTTG GACACAACTC CAGCCCTGGT TGGGGGCTGA AGGGCCAAGA TAGAAGCAGC 
CACCCTGCAG TGGGTATTTG GGGAGATTCT GGTCCACCCT TAGCTCATGT GATCTTGAGG 
GAGCCACTAT CCTCCCTAGA CAGTGGAACC AAGGAGCTGG AGCTTCCTCC ATTGTGTGCC 
ATGCATGGCA CCCTCCACAA CTTTGAAGAA ACAGGCCCGG GGCTGTACAC AGAAGACACC 
GCTCAGGTCT CAGGACCTCA GTTCCGGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spns2 ( XM_006246563.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246625.2 Rattus norvegicus spinster homolog 2 (Spns2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa50674
Clone ID Related Accession (Same CDS sequence)	XM_006246563.3
Accession Version	XM_006246563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	protein spinster homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCC TGGAATGCGC CTCGGCGGCG GCGGGCGGCG CGGAGGAGGA GGAGGCGGAC 
GCCGAACGGC GGCGCCGGCG CCGGGGGGCG CAGCCAGGGG CTGGAGGTAG CGCTTGCTGC 
GGGGCGCGGG GTGTTGGGGG CGCTGGAGTC GTGTCCGCGG ACGAAGAGGT GCAGACGTTG 
TCCGGCAGCG TGAGGAGGGT CCCGTCCGGA CTCCCCAGCA TCCCCAGTAC CCCGGGCTGC 
GCAGCCGCTG CCAAGGGCCC CGGCGCGCCG CAGCCCAAAC CCGCCAGCTT GGGCCGAGGG 
CGGGGGGCAG CCGCTGCCAT CCTGAGCTTG GGCAACGTGC TCAACTACCT GGACAGGTAC 
ACTGTGGCAG GTGTTCTTCT GGACATCCAG CAGCACTTTG GGGTCAAGGA CCGGGGCGCT 
GGCTTGCTGC AGTCAGTGTT CATCTGCAGC TTCATGGTGG CTGCCCCCAT CTTTGGCTAC 
CTGGGCGATC GCTTCAATAG AAAGGTGATC CTCAGCTGCG GCATCTTCTT CTGGTCGGCC 
GTCACCTTCT CTAGTTCTTT CATCCCTCAG CAGTACTTCT GGCTGCTGGT CCTGTCCCGG 
GGGCTAGTAG GTATTGGTGA AGCTAGCTAT TCCACCATAG CACCCACCAT CATCGGCGAC 
TTATTTACCA AGAACACACG CACACTGATG CTATCAGTCT TCTATTTTGC CATCCCTCTG 
GGCAGTGGCC TGGGCTACAT CACAGGTTCC AGCGTGAAGC AGGCAGCTGG AGACTGGCAT 
TGGGCCCTGC GGGTTTCCCC TGTCCTGGGC ATGATCACAG GAACACTCAT CCTCATCTTG 
GTCCCAGCCA CTAAGAGAGG CCATGCTGAT CAACTTGGGG GGCAGCTCAA AGCACGGACC 
TCCTGGCTCC GAGACATGAA GGCCCTGATC CGGAACCGCA GTTACGTCTT CTCCTCCCTG 
GCCACATCCG CTGTGTCCTT CGCCACAGGA GCCCTGGGCA TGTGGATTCC CCTCTATCTG 
CACCGTGCTC AAGTTGTACA AAAGACAGCA GAGACTTGCA ACAGCCCGCC CTGTGGAGCC 
AAAGACAGCC TCATCTTTGG GGCCATTACC TGCTTTACTG GCTTTCTGGG CGTAGTCACA 
GGCGCGGGAG CCACTCGCTG GTGCCGCTTG CGCACTCAGC GCGCTGACCC CTTGGTGTGC 
GCCGTGGGCA TGCTGGGATC TGCCATCTTC ATCTGCCTCA TCTTTGTGGC TGCCAAGACC 
AGCATCGTGG GCGCCTATAT CTGCATCTTT GTTGGCGAGA CACTGCTGTT TTCTAACTGG 
GCCATCACTG CAGACATCCT CATGTATGTG GTCATCCCCA CTCGACGAGC CACTGCTGTG 
GCCTTGCAGA GCTTCACCTC CCATCTGCTG GGGGACGCTG GAAGCCCCTA CCTCATTGGC 
TTTATCTCGG ACCTAATCCG CCAGAGCACC AAGGACTCCC CACTCTGGGA GTTTCTGAGC 
CTGGGCTATG CCCTCATGCT GTGCCCTTTT GTTGTGGTCC TGGGTGGCAT GTTCTTCCTT 
GCCACTGCTC TCTTCTTCCT CAGCGATCGT GCCAAGGCTG AGCAGCAGGT GAACCAGCTG 
GTGATGCCGC CTGCATCCGT GAAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246625.2
CDS	176..1825
Translation	MMCLECASAAAGGAEEEEADAERRRRRRGAQPGAGGSACCGARGVGGAGVVSADEEVQTLSGSVRRVPSGLPSIPSTPGCAAAAKGPGAPQPKPASLGRGRGAAAAILSLGNVLNYLDRYTVAGVLLDIQQHFGVKDRGAGLLQSVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSAVTFSSSFIPQQYFWLLVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQAAGDWHWALRVSPVLGMITGTLILILVPATKRGHADQLGGQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGMWIPLYLHRAQVVQKTAETCNSPPCGAKDSLIFGAITCFTGFLGVVTGAGATRWCRLRTQRADPLVCAVGMLGSAIFICLIFVAAKTSIVGAYICIFVGETLLFSNWAITADILMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIRQSTKDSPLWEFLSLGYALMLCPFVVVLGGMFFLATALFFLSDRAKAEQQVNQLVMPPASVKI

Target ORF information:

RefSeq Version	XM_006246563.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spinster homolog 2 (Spns2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246563.3

ORF Insert Sequence:

ATGATGTGCC TGGAATGCGC CTCGGCGGCG GCGGGCGGCG CGGAGGAGGA GGAGGCGGAC 
GCCGAACGGC GGCGCCGGCG CCGGGGGGCG CAGCCAGGGG CTGGAGGTAG CGCTTGCTGC 
GGGGCGCGGG GTGTTGGGGG CGCTGGAGTC GTGTCCGCGG ACGAAGAGGT GCAGACGTTG 
TCCGGCAGCG TGAGGAGGGT CCCGTCCGGA CTCCCCAGCA TCCCCAGTAC CCCGGGCTGC 
GCAGCCGCTG CCAAGGGCCC CGGCGCGCCG CAGCCCAAAC CCGCCAGCTT GGGCCGAGGG 
CGGGGGGCAG CCGCTGCCAT CCTGAGCTTG GGCAACGTGC TCAACTACCT GGACAGGTAC 
ACTGTGGCAG GTGTTCTTCT GGACATCCAG CAGCACTTTG GGGTCAAGGA CCGGGGCGCT 
GGCTTGCTGC AGTCAGTGTT CATCTGCAGC TTCATGGTGG CTGCCCCCAT CTTTGGCTAC 
CTGGGCGATC GCTTCAATAG AAAGGTGATC CTCAGCTGCG GCATCTTCTT CTGGTCGGCC 
GTCACCTTCT CTAGTTCTTT CATCCCTCAG CAGTACTTCT GGCTGCTGGT CCTGTCCCGG 
GGGCTAGTAG GTATTGGTGA AGCTAGCTAT TCCACCATAG CACCCACCAT CATCGGCGAC 
TTATTTACCA AGAACACACG CACACTGATG CTATCAGTCT TCTATTTTGC CATCCCTCTG 
GGCAGTGGCC TGGGCTACAT CACAGGTTCC AGCGTGAAGC AGGCAGCTGG AGACTGGCAT 
TGGGCCCTGC GGGTTTCCCC TGTCCTGGGC ATGATCACAG GAACACTCAT CCTCATCTTG 
GTCCCAGCCA CTAAGAGAGG CCATGCTGAT CAACTTGGGG GGCAGCTCAA AGCACGGACC 
TCCTGGCTCC GAGACATGAA GGCCCTGATC CGGAACCGCA GTTACGTCTT CTCCTCCCTG 
GCCACATCCG CTGTGTCCTT CGCCACAGGA GCCCTGGGCA TGTGGATTCC CCTCTATCTG 
CACCGTGCTC AAGTTGTACA AAAGACAGCA GAGACTTGCA ACAGCCCGCC CTGTGGAGCC 
AAAGACAGCC TCATCTTTGG GGCCATTACC TGCTTTACTG GCTTTCTGGG CGTAGTCACA 
GGCGCGGGAG CCACTCGCTG GTGCCGCTTG CGCACTCAGC GCGCTGACCC CTTGGTGTGC 
GCCGTGGGCA TGCTGGGATC TGCCATCTTC ATCTGCCTCA TCTTTGTGGC TGCCAAGACC 
AGCATCGTGG GCGCCTATAT CTGCATCTTT GTTGGCGAGA CACTGCTGTT TTCTAACTGG 
GCCATCACTG CAGACATCCT CATGTATGTG GTCATCCCCA CTCGACGAGC CACTGCTGTG 
GCCTTGCAGA GCTTCACCTC CCATCTGCTG GGGGACGCTG GAAGCCCCTA CCTCATTGGC 
TTTATCTCGG ACCTAATCCG CCAGAGCACC AAGGACTCCC CACTCTGGGA GTTTCTGAGC 
CTGGGCTATG CCCTCATGCT GTGCCCTTTT GTTGTGGTCC TGGGTGGCAT GTTCTTCCTT 
GCCACTGCTC TCTTCTTCCT CAGCGATCGT GCCAAGGCTG AGCAGCAGGT GAACCAGCTG 
GTGATGCCGC CTGCATCCGT GAAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spns2, a transporter of phosphorylated sphingoid bases, regulates their blood and lymph levels, and the lymphatic network.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(3)1001-11(2013 Mar)
Nagahashi M,Kim EY,Yamada A,Ramachandran S,Allegood JC,Hait NC,Maceyka M,Milstien S,Takabe K,Spiegel S

The role of sphingosine-1-phosphate transporter Spns2 in immune system function.
Journal of immunology (Baltimore, Md. : 1950)189(1)102-11(2012 Jul)
Nijnik A,Clare S,Hale C,Chen J,Raisen C,Mottram L,Lucas M,Estabel J,Ryder E,Adissu H,,Adams NC,Ramirez-Solis R,White JK,Steel KP,Dougan G,Hancock RE

The sphingosine-1-phosphate transporter Spns2 expressed on endothelial cells regulates lymphocyte trafficking in mice.
The Journal of clinical investigation122(4)1416-26(2012 Apr)
Fukuhara S,Simmons S,Kawamura S,Inoue A,Orba Y,Tokudome T,Sunden Y,Arai Y,Moriwaki K,Ishida J,Uemura A,Kiyonari H,Abe T,Fukamizu A,Hirashima M,Sawa H,Aoki J,Ishii M,Mochizuki N

Rapid-throughput skeletal phenotyping of 100 knockout mice identifies 9 new genes that determine bone strength.
PLoS genetics8(8)e1002858(2012)
Bassett JH,Gogakos A,White JK,Evans H,Jacques RM,van der Spek AH,,Ramirez-Solis R,Ryder E,Sunter D,Boyde A,Campbell MJ,Croucher PI,Williams GR

The sphingosine 1-phosphate transporter, SPNS2, functions as a transporter of the phosphorylated form of the immunomodulating agent FTY720.
The Journal of biological chemistry286(3)1758-66(2011 Jan)
Hisano Y,Kobayashi N,Kawahara A,Yamaguchi A,Nishi T

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Spns2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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