DOCK10 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	DOCK10
Entrez Gene ID	105294750
Full Name	dedicator of cytokinesis 10
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023532929.1	XP_023388697.1	dedicator of cytokinesis protein 10 isoform X1
XM_023532931.1	XP_023388699.1	dedicator of cytokinesis protein 10 isoform X3
XM_011364080.1	XP_011362382.1	dedicator of cytokinesis protein 10 isoform X2
XM_023532930.1	XP_023388698.1	dedicator of cytokinesis protein 10 isoform X2
XM_011364078.2	XP_011362380.1	dedicator of cytokinesis protein 10 isoform X4
XM_011364081.1	XP_011362383.1	dedicator of cytokinesis protein 10 isoform X3
XM_011364078.1	XP_011362380.1	dedicator of cytokinesis protein 10 isoform X1

Pathways from BioSystems
Gene Ontology

The following DOCK10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOCK10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt49583	XM_011364078.2 Latest version!	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt49583	XM_011364080.1 Latest version!	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt49584	XM_011364081.1 Latest version!	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt70924	XM_023532930.1 Latest version!	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt70924	XM_023532929.1 Latest version!	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPt70925	XM_023532931.1 Latest version!	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OPt49583	XM_011364078.1	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DOCK10 ( XM_011364080.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011362382.1 Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X2, mRNA.

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CloneID	OPt49583
Clone ID Related Accession (Same CDS sequence)	XM_011364080.1 , XM_011364078.2 , XM_011364078.1
Accession Version	XM_011364080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	dedicator of cytokinesis protein 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888874.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC GAGGAAAGGT TTTTAAACGA GAGCCCAGTG AATTTTGGAA GAAGAGACGA 
ACCGTGAGGA GAGTAAATTA TGAAGAAATC CACAGGCTTA GTTCTCAGGA AAAGCCCAGG 
CTCCTTGAAC CCTTGGATTA TGAAACTGTC ATTGAAGAAC TTGAAAAGAC CTATCAGAAT 
GACCCTCTTC AAGATCTCCT GTTCTTCCCC AGTGACGACT TTTCAACGGC CACAGTTCCT 
TGGGACATTC GAACACTGTA CTCTACGGTA CCTGAAGACG CAGAGCACAA AGCAGAAAAT 
TTACTGGTCA AAGAGGCTTG TAAATTTTAT AGTTCCCAGT GGTACGTGGT AAACTACAAA 
TATGAACAAT ATTCTGGAGA TATTCGCCAG TTACCCCGAG CAGAATACAA ACCAGAGAAA 
CTCCCTTCGC ATTCCTTTGA GGTTGACCAT GAAGATGCTG ATAAGGATGA AGATACCACC 
TCCCACTCAT CTTCCAAGGG CGGTGGGGGA GCAGGGGGAA CTGGAGTCTT CAAGTCAGGC 
TGGCTCTACA AGGGGAATTT CAACAGCACC GTGAACAATA CCATCACTGT TCGGTCATTC 
AAGAAGCGCT ACTTCCAGCT GACTCAGCTG CCAGATAACT CCTACATCAT GAATTTTTAC 
AAAGATGAAA AAATATCCAA AGAGCCCAAA GGCTGCATCT TTTTGGATTC CTGTACAGGT 
GTGGTGCAGA ATAACAGACT AAGAAAATAT GCCTTTGAAT TGAAAATGAA TGACCTGACC 
TATTTTGTGC TGGCGGCGGA AACAGAGTGC GACATGGAGG AATGGATCCA CACCCTGAAC 
CGCATCCTGC AAATCAGCCC TGAGGGGCCC CTGCAGGGTC GACGGAGCGC GGAGCTCACG 
GATCTGGGCC TGGATTCACT AGATAATTCT ATCACATGTG AATGCGCACC AGAGGAAACA 
GATTCTTCAG AGAACAGCCT ACATCCAGAC TTCGCAAAAT ACCTCACAGA AACTGAAGAG 
ACTGTCAAAG CAACTCGAAA CATGGAGAGA CTCAATCTGT TCTCGATGGA CCCAGATATA 
GATACCTTGA AACTTCAGAA AAAGGATCTT TTGGAATCCG AGTCTGTGAT CAAACCATTT 
GAAGAAAAAG CTGCCAAGAG AATCATGATC ATATGTAAAG CTCTCACCTT CAGTCTTCAG 
GGATGTGTTA CAGAGAATGA AAATGATCCC ATAACCAACA TTGAGCCTTT TTTTGTGAGT 
GTGGCACTTT ATGACCTCAG AAACAGCAGG AAGATTTCTG CCGACTTCCA CGTGGATCTA 
AACCACACTG CTGTCAGGCA GATGCTCTCA GGGGCTCCCG TGGCTTTGGA AAATGGAAAC 
AGCGACACGG TCACCCCAAG ACAATCAGAA GAACCTCACG TCAAGGGGTT TCCAGAGGAA 
TGGCTGAAAT TTCCAAAGCA GGCTATATTT TCTGTAAGCA ATCCACATTC GGAAATTGTC 
TTGGTGGCTA AAATTGAAAA AGTACTAATG GGAAACATTG CAAGTGGTGC TGAACCTTAC 
ATTAAGAACC CAGACTCCAA CAAGTATGCA CAAAAGATAC TCAAATCCAA CAGACAGTTC 
TGCAGCAAGT TGGGGAAATA CCGCATGCCG TTTGCTTGGG CGATGAGATC AGTTTTTAAG 
GACAACCAGG GAAATGTGGA CAGAGACTCA AGATTTTCAC CATTGTATAG ACACGAAAGT 
AGCAAGATTT CAACTGAGGA CCTACTTAAA CTAGTGTCAG ATTATAGGAG GGCCGACAGA 
ATAAGCAAAA TGCAGACCAT TCCAGGAAGC CTCGATATCA CTGTTGAGAA CATTCCTCTG 
GAGCATCCAA ACTGTGTAAC ATCGTCCTTT ATCCCCGTCA AGCCTTTCAA CGTGACGGCT 
CAACCGGAAC CCACAGTGGA GGTGGAAGAA TTTGTTTATG ACTCAACAAA GTACTGCCGT 
CCTTACAGAG TATACAAAAA TCAAATTTAC ATTTACCCCA AGCACCTCAA GTATGATAGC 
CAGAAATGTT TCAACAAGGC ACGAAATATA ACCGTGTGCA TTGAATTCAA AAATTCTGAC 
GACGAAGGTG CCAAGCCCAT GAAGTGTATT TATGGGAAGC CTGGAGGGCC CCTCTTCACC 
TCAGCCGCCT ACACAGCAGT TCTGCACCAT TCCCAGAATC CGGATTTCTC AGACGAGGTG 
AAAATTGAGC TACCAACACA ACTCCACGAG AAACACCATA TTCTGTTTTC TTTTTATCAT 
GTCACTTGCG ACATAAATGC AAAAGCTAAT GCCAAAAAGA AGGAAGTTCT GGAAACATCA 
GTTGGATATG CTTGGCTTCC TCTAATGAAA CATGATCAGA TAGCTTCTCA AGAGTACAAT 
ATCCCAATAG CTACAAGCCT GCCTCCTAAT TATTTAAGCT TTCAAGATTC TGCAAGTGGA 
AAGCATGGTG GGAGTGACAT TAAATGGGTT GATGGTGGCA AACCACTTTT CAAAGTATCA 
ACATTCGTTG TATCAACAGT GAATACTCAG GATCCGTATG TGAACGCGTT TTTCCGACAG 
TGCCAAAAAA GAGAGAAAGA TATGTCTCAG TCACCTACCT CGAATTTCAT CCGCTCTTGT 
AAGAACTTAT TGAATGTGGA AAAAATTCAC GCAATCATGA GTTTTCTGCC TATAATCTTG 
AATCAGCTCT TCAAGGTTTT GGTACAGAAT GAGGAAGATG AAATAAGTAC AACTGTGACC 
AGGGTTCTGA CTGACATTGT GGCCAAGTGC CACGAGGAGC AGCTGGACCA CTCCGTCCAG 
TCATACATTA AGTTTGTGTT CAAGACCAAG GCTTGCAAGG AGAGAACGAT ACACGAGGAA 
CTGACTAAGA GTGTGACTGG TCTTTTGAAA TCAAATGACT CCACAACAGT CAGGCACGTT 
CTGAAGCATT CCTGGTTCTT CTTCGCAATT ATTCTAAAAT CGATGGCACA ACACTTGATT 
GATACAAATA AAATTCAGCT TTCTCGGGCT CAAAGATTTC CTGAATCTTA CCAGAATGAA 
TTGGACAATC TTATAATGGT CCTATCTGAT CACGTGATCT GGAAATACAA AGATGCCCTT 
GAAGAAACAA GAAGGGCCAA CCACAGCGTT GCCAGATTTC TCAAGCGCTG CTTCACCTTC 
ATGGATCGGG GCTTTGTGTT TAAGATGGTT AACAATTACA TCAGCATGTT CTCCTCTGGT 
GAGCTCAAGA CCTTATGCCA GTATAAATTT GATTTTCTTC AAGAAGTATG TCAGCATGAA 
CATTTTATTC CTTTGTGTCT CCCCATAAGA TCAGCAAACA TTCCAGATCC TCTGACACCT 
TCGGAATCAA CTCAAGAGCT ACATGCATCA GATATGCCTG AATATTCGGT CACGAATGAA 
TTTTGCCGCA AGCACTTCTT AATCGGGATT CTGCTCCGAG AAGTTGGCTT TGCTCTGCAG 
GAAGACCAAG ATATCAGGCA CTTAGCTTTA GCTGTCCTGA AAAATCTAAT GGCTAAGCAT 
TCGTTTGATG ATCGATACAG AGAACCAAGA AAGCAGGCCC AGATAGCAAG CTTATACATG 
CCCCTCTATG GCATGCTTCT GGACAACATG CCCAGGATTT ACCTGAAGGA TCTATATCCT 
TTCACTATCA ATACATCAAA CCAGGGGTCT AGAGATGACC TCAGCACCAA CGGAGGATTT 
CACAGCCAGT CAGCTATGAA ACATGCAAAC TCTGTGGACA CATCATTTTC CAAAGATGTT 
TTAAATTCCA TAGCAGCATT TTCATCAATA GCTATTTCTA CAGTAAACCA TGCTGATTCC 
AGAGCATCCT TAGCAAGTCT GGACTCCAAT CCAAGTACCA ATGAGAAGAG CAGTGAGAAA 
ACTGACAACT GTGAAAAGAT CCCAAGACCC TTGTCTTTGA TAGGCTCAAC GCTTCGATTT 
GACAAGTTAG ATCAAGCAGA AACCAGGAGC CTTCTTATGT GCTTTCTTCA AATTATGAAG 
ACAATTTCAG AGGAGACTCT GATTGCCTAC TGGCAAAGAG CACCTAGTCC GGAAGTGTCT 
GACTTCTTCA GCATCTTGGA TGTTTGTCTT CAAAATTTCA GATACCTAGG AAAACGCAAT 
ATAATAAGGA AAATTGCTGC TGCATTTAAA TTTGTGCAGT CCACCCAGAA CAACGGAACT 
CTCAAAGGAT CCAATCCTTC TTGCCAAACA TCGGGTCTCT TGACACAATG GATGAACACC 
ACTTCTGGTC ACGAAGGACA TAAGCAGCAC AGATCACAAA CTTTACCTAT AATTCGAGGC 
AAAAATGCAC TCTCTAACCC CAAACTCTTA CAGATGCTAG ACAATACCAT GACCAGCAAC 
TCCAATGAAA TAGACATCGT GCACCACGTG GACACAGAGG CCAATGTAGC TACAGAGGTT 
TGCCTTACTA TTCTAGACCT GCTGTCCCTC TTCACTCAGA TTCACCATAG GCAACTCCAA 
CAATCTGAAT GTCAAAATTC ATTGATGAAA AGGGTCTTTG ATACCTACAT GCTCTTCTTC 
CAAGTCAACC AGTCAGCCAC AGCACTGAAG CATGTGTTTG CTTCCCTGAG ACTGTTTGTA 
TGCAAGTTTC CCGCAGCGTT CTTTCAAGGG CCTGCTGACC TGTGTGGATC ATTCTGTTAC 
GAAGTCCTAA AATGCTGTAA CCACAGGTCA CGGTCAACCC AGACAGAAGC ATCGGCACTT 
CTATACTTTT TCATGAGGAA GAATTTTGAA TTTAATAAGC AGAAGTCAAT TGTCCGATCC 
CACCTACAGC TCATCAAGGC TGTAAGCCAG TTAATAGCCG ATGCTGGGAT TGGAGGCTCT 
CGGTTTCAAC ATTCCCTTGC AATTACCAAT AATTTTGCTA ATGGAGACAA GCAAATGAAA 
AACAGCAATT TCCCAGCAGA GGTGAAAGAT CTGACTAAAC GTATAAGGAC TGTTCTGATG 
GCCACGGCTC AGATGAAGGA GCATGAGAAG GACCCCGAGA TGCTGGTGGA CCTCCAGTAT 
AGCCTGGCAA ACTCCTATGC GAGCACCCCT GAGCTACGGA GGACTTGGCT GGAAAGCATG 
GCCAAGATTC ACGCAAGAAA TGGAGACTTG TCCGAGGCTG CCATGTGCTA CATCCACATA 
GCAGCCCTCA TCGCAGAATA CCTGAAAAGA AAGGGTTACT GGAAAATGGA AAAGATTTGC 
ACGCCATCCC TGCTCCTGGA GGATATCCAT CCCTGTGATA GCATCCCATT ACTAACAACT 
CCCAGTGGAG GAAGCATGTT CTCCATGGGC TGGCCAGCTT TTCTGAGCAT TACACCAAAT 
ATTAAAGAAG AAGGAGCAAT GAAAGAAGAT TCGGGAATGC AAGACACACC ATACAATGAG 
AATATCCTGG TGGAACAGCT GTATATGTGT GTGGAGTTTC TTTGGAAGTC TGAGAGATAT 
GAACTCATCG CTGATGTCAA CAAACCCATC ATCGCTGTCT TTGAGAAGCA GAGAGACTTC 
AAAAAATTGT CAGATCTCTA CTATGACATT CACCGGTCAT ACCTGAAAGT CGCAGAGGTG 
GTGAATTCAG AGAAGCGACT GTTTGGTCGG TACTATCGCG TGGCGTTTTA CGGGCAGGGG 
TTTTTTGAAG AAGAAGAAGG TAAAGAGTAT ATTTATAAAG AGCCTAAGCT GACAGGTCTC 
TCCGAGATTT CCCAAAGACT CCTCAAGCTG TATGCAGATA AATTCGGAGC AGACAATGTG 
AAGATAATCC AGGATTCCAA CAAGGTAAAC CCGAAGGACT TGGACCCCAA ATATGCCTAC 
ATCCAGGTGA CCTACGTCAC TCCATTCTTT GAGGAAAAGG AAATAGAGGA CCGGAAGACG 
GATTTCGAAA TGCACCACAA CATCAAGCGC TTTGTCTTTG AGACGCCCTT CACGCTGTCG 
GGCAAGAAGC ATGGTGGCGT GGAGGAGCAG TGCAAGCGAC GGACGGTGCT GACCACAAGT 
CACCTGTTCC CCTACGTGAA GAAGAGAATA CAGGTCATAA GCCAGTCGAG CACAGAACTG 
AATCCTATTG AGGTGGCGAT TGATGAGATG TCCAAGAAAG TTTCTGAGCT TAATCAGCTC 
TGCACGATGG AGGAAGTGGA CATGATCAGA CTGCAGCTCA AACTCCAAGG AAGTGTCAGC 
GTGAAGGTTA ATGCTGGACC AATGGCCTAT GCGCGAGCTT TTCTTGAAGA AACCAACGCA 
AAGAAATACC CTGACAACCA AGTAAAGCTT TTGAAGGAAA TCTTCAGGCA ATTTGCAGAT 
GCGTGTGGGC AGGCCCTGGA CGTGAATGAG CGCCTCATCA AGGAGGACCA GCTGGAGTAC 
CAGGAAGAAC TGAGGTCTCA TTATAAGGAC ATGCTCGGCG AACTCTCTGC AATTATGAAT 
GAGCAGATTA CGTACAGGGA CGACCTGGCA AAGCGCGGAT TGGAACGAAC CTTCACTCGA 
GTAACTAGCA AGGCAACTGC AGCTCCGCCC ACGGTGTCCA TCTCACCTTG CGTGGAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011362382.1
CDS	136..6678
Translation	MSFRGKVFKREPSEFWKKRRTVRRVNYEEIHRLSSQEKPRLLEPLDYETVIEELEKTYQNDPLQDLLFFPSDDFSTATVPWDIRTLYSTVPEDAEHKAENLLVKEACKFYSSQWYVVNYKYEQYSGDIRQLPRAEYKPEKLPSHSFEVDHEDADKDEDTTSHSSSKGGGGAGGTGVFKSGWLYKGNFNSTVNNTITVRSFKKRYFQLTQLPDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKYAFELKMNDLTYFVLAAETECDMEEWIHTLNRILQISPEGPLQGRRSAELTDLGLDSLDNSITCECAPEETDSSENSLHPDFAKYLTETEETVKATRNMERLNLFSMDPDIDTLKLQKKDLLESESVIKPFEEKAAKRIMIICKALTFSLQGCVTENENDPITNIEPFFVSVALYDLRNSRKISADFHVDLNHTAVRQMLSGAPVALENGNSDTVTPRQSEEPHVKGFPEEWLKFPKQAIFSVSNPHSEIVLVAKIEKVLMGNIASGAEPYIKNPDSNKYAQKILKSNRQFCSKLGKYRMPFAWAMRSVFKDNQGNVDRDSRFSPLYRHESSKISTEDLLKLVSDYRRADRISKMQTIPGSLDITVENIPLEHPNCVTSSFIPVKPFNVTAQPEPTVEVEEFVYDSTKYCRPYRVYKNQIYIYPKHLKYDSQKCFNKARNITVCIEFKNSDDEGAKPMKCIYGKPGGPLFTSAAYTAVLHHSQNPDFSDEVKIELPTQLHEKHHILFSFYHVTCDINAKANAKKKEVLETSVGYAWLPLMKHDQIASQEYNIPIATSLPPNYLSFQDSASGKHGGSDIKWVDGGKPLFKVSTFVVSTVNTQDPYVNAFFRQCQKREKDMSQSPTSNFIRSCKNLLNVEKIHAIMSFLPIILNQLFKVLVQNEEDEISTTVTRVLTDIVAKCHEEQLDHSVQSYIKFVFKTKACKERTIHEELTKSVTGLLKSNDSTTVRHVLKHSWFFFAIILKSMAQHLIDTNKIQLSRAQRFPESYQNELDNLIMVLSDHVIWKYKDALEETRRANHSVARFLKRCFTFMDRGFVFKMVNNYISMFSSGELKTLCQYKFDFLQEVCQHEHFIPLCLPIRSANIPDPLTPSESTQELHASDMPEYSVTNEFCRKHFLIGILLREVGFALQEDQDIRHLALAVLKNLMAKHSFDDRYREPRKQAQIASLYMPLYGMLLDNMPRIYLKDLYPFTINTSNQGSRDDLSTNGGFHSQSAMKHANSVDTSFSKDVLNSIAAFSSIAISTVNHADSRASLASLDSNPSTNEKSSEKTDNCEKIPRPLSLIGSTLRFDKLDQAETRSLLMCFLQIMKTISEETLIAYWQRAPSPEVSDFFSILDVCLQNFRYLGKRNIIRKIAAAFKFVQSTQNNGTLKGSNPSCQTSGLLTQWMNTTSGHEGHKQHRSQTLPIIRGKNALSNPKLLQMLDNTMTSNSNEIDIVHHVDTEANVATEVCLTILDLLSLFTQIHHRQLQQSECQNSLMKRVFDTYMLFFQVNQSATALKHVFASLRLFVCKFPAAFFQGPADLCGSFCYEVLKCCNHRSRSTQTEASALLYFFMRKNFEFNKQKSIVRSHLQLIKAVSQLIADAGIGGSRFQHSLAITNNFANGDKQMKNSNFPAEVKDLTKRIRTVLMATAQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHARNGDLSEAAMCYIHIAALIAEYLKRKGYWKMEKICTPSLLLEDIHPCDSIPLLTTPSGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQDTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVNPKDLDPKYAYIQVTYVTPFFEEKEIEDRKTDFEMHHNIKRFVFETPFTLSGKKHGGVEEQCKRRTVLTTSHLFPYVKKRIQVISQSSTELNPIEVAIDEMSKKVSELNQLCTMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEETNAKKYPDNQVKLLKEIFRQFADACGQALDVNERLIKEDQLEYQEELRSHYKDMLGELSAIMNEQITYRDDLAKRGLERTFTRVTSKATAAPPTVSISPCVEI

Target ORF information:

RefSeq Version	XM_011364080.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011364080.1

ORF Insert Sequence:

ATGAGCTTCC GAGGAAAGGT TTTTAAACGA GAGCCCAGTG AATTTTGGAA GAAGAGACGA 
ACCGTGAGGA GAGTAAATTA TGAAGAAATC CACAGGCTTA GTTCTCAGGA AAAGCCCAGG 
CTCCTTGAAC CCTTGGATTA TGAAACTGTC ATTGAAGAAC TTGAAAAGAC CTATCAGAAT 
GACCCTCTTC AAGATCTCCT GTTCTTCCCC AGTGACGACT TTTCAACGGC CACAGTTCCT 
TGGGACATTC GAACACTGTA CTCTACGGTA CCTGAAGACG CAGAGCACAA AGCAGAAAAT 
TTACTGGTCA AAGAGGCTTG TAAATTTTAT AGTTCCCAGT GGTACGTGGT AAACTACAAA 
TATGAACAAT ATTCTGGAGA TATTCGCCAG TTACCCCGAG CAGAATACAA ACCAGAGAAA 
CTCCCTTCGC ATTCCTTTGA GGTTGACCAT GAAGATGCTG ATAAGGATGA AGATACCACC 
TCCCACTCAT CTTCCAAGGG CGGTGGGGGA GCAGGGGGAA CTGGAGTCTT CAAGTCAGGC 
TGGCTCTACA AGGGGAATTT CAACAGCACC GTGAACAATA CCATCACTGT TCGGTCATTC 
AAGAAGCGCT ACTTCCAGCT GACTCAGCTG CCAGATAACT CCTACATCAT GAATTTTTAC 
AAAGATGAAA AAATATCCAA AGAGCCCAAA GGCTGCATCT TTTTGGATTC CTGTACAGGT 
GTGGTGCAGA ATAACAGACT AAGAAAATAT GCCTTTGAAT TGAAAATGAA TGACCTGACC 
TATTTTGTGC TGGCGGCGGA AACAGAGTGC GACATGGAGG AATGGATCCA CACCCTGAAC 
CGCATCCTGC AAATCAGCCC TGAGGGGCCC CTGCAGGGTC GACGGAGCGC GGAGCTCACG 
GATCTGGGCC TGGATTCACT AGATAATTCT ATCACATGTG AATGCGCACC AGAGGAAACA 
GATTCTTCAG AGAACAGCCT ACATCCAGAC TTCGCAAAAT ACCTCACAGA AACTGAAGAG 
ACTGTCAAAG CAACTCGAAA CATGGAGAGA CTCAATCTGT TCTCGATGGA CCCAGATATA 
GATACCTTGA AACTTCAGAA AAAGGATCTT TTGGAATCCG AGTCTGTGAT CAAACCATTT 
GAAGAAAAAG CTGCCAAGAG AATCATGATC ATATGTAAAG CTCTCACCTT CAGTCTTCAG 
GGATGTGTTA CAGAGAATGA AAATGATCCC ATAACCAACA TTGAGCCTTT TTTTGTGAGT 
GTGGCACTTT ATGACCTCAG AAACAGCAGG AAGATTTCTG CCGACTTCCA CGTGGATCTA 
AACCACACTG CTGTCAGGCA GATGCTCTCA GGGGCTCCCG TGGCTTTGGA AAATGGAAAC 
AGCGACACGG TCACCCCAAG ACAATCAGAA GAACCTCACG TCAAGGGGTT TCCAGAGGAA 
TGGCTGAAAT TTCCAAAGCA GGCTATATTT TCTGTAAGCA ATCCACATTC GGAAATTGTC 
TTGGTGGCTA AAATTGAAAA AGTACTAATG GGAAACATTG CAAGTGGTGC TGAACCTTAC 
ATTAAGAACC CAGACTCCAA CAAGTATGCA CAAAAGATAC TCAAATCCAA CAGACAGTTC 
TGCAGCAAGT TGGGGAAATA CCGCATGCCG TTTGCTTGGG CGATGAGATC AGTTTTTAAG 
GACAACCAGG GAAATGTGGA CAGAGACTCA AGATTTTCAC CATTGTATAG ACACGAAAGT 
AGCAAGATTT CAACTGAGGA CCTACTTAAA CTAGTGTCAG ATTATAGGAG GGCCGACAGA 
ATAAGCAAAA TGCAGACCAT TCCAGGAAGC CTCGATATCA CTGTTGAGAA CATTCCTCTG 
GAGCATCCAA ACTGTGTAAC ATCGTCCTTT ATCCCCGTCA AGCCTTTCAA CGTGACGGCT 
CAACCGGAAC CCACAGTGGA GGTGGAAGAA TTTGTTTATG ACTCAACAAA GTACTGCCGT 
CCTTACAGAG TATACAAAAA TCAAATTTAC ATTTACCCCA AGCACCTCAA GTATGATAGC 
CAGAAATGTT TCAACAAGGC ACGAAATATA ACCGTGTGCA TTGAATTCAA AAATTCTGAC 
GACGAAGGTG CCAAGCCCAT GAAGTGTATT TATGGGAAGC CTGGAGGGCC CCTCTTCACC 
TCAGCCGCCT ACACAGCAGT TCTGCACCAT TCCCAGAATC CGGATTTCTC AGACGAGGTG 
AAAATTGAGC TACCAACACA ACTCCACGAG AAACACCATA TTCTGTTTTC TTTTTATCAT 
GTCACTTGCG ACATAAATGC AAAAGCTAAT GCCAAAAAGA AGGAAGTTCT GGAAACATCA 
GTTGGATATG CTTGGCTTCC TCTAATGAAA CATGATCAGA TAGCTTCTCA AGAGTACAAT 
ATCCCAATAG CTACAAGCCT GCCTCCTAAT TATTTAAGCT TTCAAGATTC TGCAAGTGGA 
AAGCATGGTG GGAGTGACAT TAAATGGGTT GATGGTGGCA AACCACTTTT CAAAGTATCA 
ACATTCGTTG TATCAACAGT GAATACTCAG GATCCGTATG TGAACGCGTT TTTCCGACAG 
TGCCAAAAAA GAGAGAAAGA TATGTCTCAG TCACCTACCT CGAATTTCAT CCGCTCTTGT 
AAGAACTTAT TGAATGTGGA AAAAATTCAC GCAATCATGA GTTTTCTGCC TATAATCTTG 
AATCAGCTCT TCAAGGTTTT GGTACAGAAT GAGGAAGATG AAATAAGTAC AACTGTGACC 
AGGGTTCTGA CTGACATTGT GGCCAAGTGC CACGAGGAGC AGCTGGACCA CTCCGTCCAG 
TCATACATTA AGTTTGTGTT CAAGACCAAG GCTTGCAAGG AGAGAACGAT ACACGAGGAA 
CTGACTAAGA GTGTGACTGG TCTTTTGAAA TCAAATGACT CCACAACAGT CAGGCACGTT 
CTGAAGCATT CCTGGTTCTT CTTCGCAATT ATTCTAAAAT CGATGGCACA ACACTTGATT 
GATACAAATA AAATTCAGCT TTCTCGGGCT CAAAGATTTC CTGAATCTTA CCAGAATGAA 
TTGGACAATC TTATAATGGT CCTATCTGAT CACGTGATCT GGAAATACAA AGATGCCCTT 
GAAGAAACAA GAAGGGCCAA CCACAGCGTT GCCAGATTTC TCAAGCGCTG CTTCACCTTC 
ATGGATCGGG GCTTTGTGTT TAAGATGGTT AACAATTACA TCAGCATGTT CTCCTCTGGT 
GAGCTCAAGA CCTTATGCCA GTATAAATTT GATTTTCTTC AAGAAGTATG TCAGCATGAA 
CATTTTATTC CTTTGTGTCT CCCCATAAGA TCAGCAAACA TTCCAGATCC TCTGACACCT 
TCGGAATCAA CTCAAGAGCT ACATGCATCA GATATGCCTG AATATTCGGT CACGAATGAA 
TTTTGCCGCA AGCACTTCTT AATCGGGATT CTGCTCCGAG AAGTTGGCTT TGCTCTGCAG 
GAAGACCAAG ATATCAGGCA CTTAGCTTTA GCTGTCCTGA AAAATCTAAT GGCTAAGCAT 
TCGTTTGATG ATCGATACAG AGAACCAAGA AAGCAGGCCC AGATAGCAAG CTTATACATG 
CCCCTCTATG GCATGCTTCT GGACAACATG CCCAGGATTT ACCTGAAGGA TCTATATCCT 
TTCACTATCA ATACATCAAA CCAGGGGTCT AGAGATGACC TCAGCACCAA CGGAGGATTT 
CACAGCCAGT CAGCTATGAA ACATGCAAAC TCTGTGGACA CATCATTTTC CAAAGATGTT 
TTAAATTCCA TAGCAGCATT TTCATCAATA GCTATTTCTA CAGTAAACCA TGCTGATTCC 
AGAGCATCCT TAGCAAGTCT GGACTCCAAT CCAAGTACCA ATGAGAAGAG CAGTGAGAAA 
ACTGACAACT GTGAAAAGAT CCCAAGACCC TTGTCTTTGA TAGGCTCAAC GCTTCGATTT 
GACAAGTTAG ATCAAGCAGA AACCAGGAGC CTTCTTATGT GCTTTCTTCA AATTATGAAG 
ACAATTTCAG AGGAGACTCT GATTGCCTAC TGGCAAAGAG CACCTAGTCC GGAAGTGTCT 
GACTTCTTCA GCATCTTGGA TGTTTGTCTT CAAAATTTCA GATACCTAGG AAAACGCAAT 
ATAATAAGGA AAATTGCTGC TGCATTTAAA TTTGTGCAGT CCACCCAGAA CAACGGAACT 
CTCAAAGGAT CCAATCCTTC TTGCCAAACA TCGGGTCTCT TGACACAATG GATGAACACC 
ACTTCTGGTC ACGAAGGACA TAAGCAGCAC AGATCACAAA CTTTACCTAT AATTCGAGGC 
AAAAATGCAC TCTCTAACCC CAAACTCTTA CAGATGCTAG ACAATACCAT GACCAGCAAC 
TCCAATGAAA TAGACATCGT GCACCACGTG GACACAGAGG CCAATGTAGC TACAGAGGTT 
TGCCTTACTA TTCTAGACCT GCTGTCCCTC TTCACTCAGA TTCACCATAG GCAACTCCAA 
CAATCTGAAT GTCAAAATTC ATTGATGAAA AGGGTCTTTG ATACCTACAT GCTCTTCTTC 
CAAGTCAACC AGTCAGCCAC AGCACTGAAG CATGTGTTTG CTTCCCTGAG ACTGTTTGTA 
TGCAAGTTTC CCGCAGCGTT CTTTCAAGGG CCTGCTGACC TGTGTGGATC ATTCTGTTAC 
GAAGTCCTAA AATGCTGTAA CCACAGGTCA CGGTCAACCC AGACAGAAGC ATCGGCACTT 
CTATACTTTT TCATGAGGAA GAATTTTGAA TTTAATAAGC AGAAGTCAAT TGTCCGATCC 
CACCTACAGC TCATCAAGGC TGTAAGCCAG TTAATAGCCG ATGCTGGGAT TGGAGGCTCT 
CGGTTTCAAC ATTCCCTTGC AATTACCAAT AATTTTGCTA ATGGAGACAA GCAAATGAAA 
AACAGCAATT TCCCAGCAGA GGTGAAAGAT CTGACTAAAC GTATAAGGAC TGTTCTGATG 
GCCACGGCTC AGATGAAGGA GCATGAGAAG GACCCCGAGA TGCTGGTGGA CCTCCAGTAT 
AGCCTGGCAA ACTCCTATGC GAGCACCCCT GAGCTACGGA GGACTTGGCT GGAAAGCATG 
GCCAAGATTC ACGCAAGAAA TGGAGACTTG TCCGAGGCTG CCATGTGCTA CATCCACATA 
GCAGCCCTCA TCGCAGAATA CCTGAAAAGA AAGGGTTACT GGAAAATGGA AAAGATTTGC 
ACGCCATCCC TGCTCCTGGA GGATATCCAT CCCTGTGATA GCATCCCATT ACTAACAACT 
CCCAGTGGAG GAAGCATGTT CTCCATGGGC TGGCCAGCTT TTCTGAGCAT TACACCAAAT 
ATTAAAGAAG AAGGAGCAAT GAAAGAAGAT TCGGGAATGC AAGACACACC ATACAATGAG 
AATATCCTGG TGGAACAGCT GTATATGTGT GTGGAGTTTC TTTGGAAGTC TGAGAGATAT 
GAACTCATCG CTGATGTCAA CAAACCCATC ATCGCTGTCT TTGAGAAGCA GAGAGACTTC 
AAAAAATTGT CAGATCTCTA CTATGACATT CACCGGTCAT ACCTGAAAGT CGCAGAGGTG 
GTGAATTCAG AGAAGCGACT GTTTGGTCGG TACTATCGCG TGGCGTTTTA CGGGCAGGGG 
TTTTTTGAAG AAGAAGAAGG TAAAGAGTAT ATTTATAAAG AGCCTAAGCT GACAGGTCTC 
TCCGAGATTT CCCAAAGACT CCTCAAGCTG TATGCAGATA AATTCGGAGC AGACAATGTG 
AAGATAATCC AGGATTCCAA CAAGGTAAAC CCGAAGGACT TGGACCCCAA ATATGCCTAC 
ATCCAGGTGA CCTACGTCAC TCCATTCTTT GAGGAAAAGG AAATAGAGGA CCGGAAGACG 
GATTTCGAAA TGCACCACAA CATCAAGCGC TTTGTCTTTG AGACGCCCTT CACGCTGTCG 
GGCAAGAAGC ATGGTGGCGT GGAGGAGCAG TGCAAGCGAC GGACGGTGCT GACCACAAGT 
CACCTGTTCC CCTACGTGAA GAAGAGAATA CAGGTCATAA GCCAGTCGAG CACAGAACTG 
AATCCTATTG AGGTGGCGAT TGATGAGATG TCCAAGAAAG TTTCTGAGCT TAATCAGCTC 
TGCACGATGG AGGAAGTGGA CATGATCAGA CTGCAGCTCA AACTCCAAGG AAGTGTCAGC 
GTGAAGGTTA ATGCTGGACC AATGGCCTAT GCGCGAGCTT TTCTTGAAGA AACCAACGCA 
AAGAAATACC CTGACAACCA AGTAAAGCTT TTGAAGGAAA TCTTCAGGCA ATTTGCAGAT 
GCGTGTGGGC AGGCCCTGGA CGTGAATGAG CGCCTCATCA AGGAGGACCA GCTGGAGTAC 
CAGGAAGAAC TGAGGTCTCA TTATAAGGAC ATGCTCGGCG AACTCTCTGC AATTATGAAT 
GAGCAGATTA CGTACAGGGA CGACCTGGCA AAGCGCGGAT TGGAACGAAC CTTCACTCGA 
GTAACTAGCA AGGCAACTGC AGCTCCGCCC ACGGTGTCCA TCTCACCTTG CGTGGAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_011364078.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011362380.1 Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X4, mRNA.

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CloneID	OPt49583
Clone ID Related Accession (Same CDS sequence)	XM_011364080.1 , XM_011364078.2 , XM_011364078.1
Accession Version	XM_011364078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	dedicator of cytokinesis protein 10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888874.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011364078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC GAGGAAAGGT TTTTAAACGA GAGCCCAGTG AATTTTGGAA GAAGAGACGA 
ACCGTGAGGA GAGTAAATTA TGAAGAAATC CACAGGCTTA GTTCTCAGGA AAAGCCCAGG 
CTCCTTGAAC CCTTGGATTA TGAAACTGTC ATTGAAGAAC TTGAAAAGAC CTATCAGAAT 
GACCCTCTTC AAGATCTCCT GTTCTTCCCC AGTGACGACT TTTCAACGGC CACAGTTCCT 
TGGGACATTC GAACACTGTA CTCTACGGTA CCTGAAGACG CAGAGCACAA AGCAGAAAAT 
TTACTGGTCA AAGAGGCTTG TAAATTTTAT AGTTCCCAGT GGTACGTGGT AAACTACAAA 
TATGAACAAT ATTCTGGAGA TATTCGCCAG TTACCCCGAG CAGAATACAA ACCAGAGAAA 
CTCCCTTCGC ATTCCTTTGA GGTTGACCAT GAAGATGCTG ATAAGGATGA AGATACCACC 
TCCCACTCAT CTTCCAAGGG CGGTGGGGGA GCAGGGGGAA CTGGAGTCTT CAAGTCAGGC 
TGGCTCTACA AGGGGAATTT CAACAGCACC GTGAACAATA CCATCACTGT TCGGTCATTC 
AAGAAGCGCT ACTTCCAGCT GACTCAGCTG CCAGATAACT CCTACATCAT GAATTTTTAC 
AAAGATGAAA AAATATCCAA AGAGCCCAAA GGCTGCATCT TTTTGGATTC CTGTACAGGT 
GTGGTGCAGA ATAACAGACT AAGAAAATAT GCCTTTGAAT TGAAAATGAA TGACCTGACC 
TATTTTGTGC TGGCGGCGGA AACAGAGTGC GACATGGAGG AATGGATCCA CACCCTGAAC 
CGCATCCTGC AAATCAGCCC TGAGGGGCCC CTGCAGGGTC GACGGAGCGC GGAGCTCACG 
GATCTGGGCC TGGATTCACT AGATAATTCT ATCACATGTG AATGCGCACC AGAGGAAACA 
GATTCTTCAG AGAACAGCCT ACATCCAGAC TTCGCAAAAT ACCTCACAGA AACTGAAGAG 
ACTGTCAAAG CAACTCGAAA CATGGAGAGA CTCAATCTGT TCTCGATGGA CCCAGATATA 
GATACCTTGA AACTTCAGAA AAAGGATCTT TTGGAATCCG AGTCTGTGAT CAAACCATTT 
GAAGAAAAAG CTGCCAAGAG AATCATGATC ATATGTAAAG CTCTCACCTT CAGTCTTCAG 
GGATGTGTTA CAGAGAATGA AAATGATCCC ATAACCAACA TTGAGCCTTT TTTTGTGAGT 
GTGGCACTTT ATGACCTCAG AAACAGCAGG AAGATTTCTG CCGACTTCCA CGTGGATCTA 
AACCACACTG CTGTCAGGCA GATGCTCTCA GGGGCTCCCG TGGCTTTGGA AAATGGAAAC 
AGCGACACGG TCACCCCAAG ACAATCAGAA GAACCTCACG TCAAGGGGTT TCCAGAGGAA 
TGGCTGAAAT TTCCAAAGCA GGCTATATTT TCTGTAAGCA ATCCACATTC GGAAATTGTC 
TTGGTGGCTA AAATTGAAAA AGTACTAATG GGAAACATTG CAAGTGGTGC TGAACCTTAC 
ATTAAGAACC CAGACTCCAA CAAGTATGCA CAAAAGATAC TCAAATCCAA CAGACAGTTC 
TGCAGCAAGT TGGGGAAATA CCGCATGCCG TTTGCTTGGG CGATGAGATC AGTTTTTAAG 
GACAACCAGG GAAATGTGGA CAGAGACTCA AGATTTTCAC CATTGTATAG ACACGAAAGT 
AGCAAGATTT CAACTGAGGA CCTACTTAAA CTAGTGTCAG ATTATAGGAG GGCCGACAGA 
ATAAGCAAAA TGCAGACCAT TCCAGGAAGC CTCGATATCA CTGTTGAGAA CATTCCTCTG 
GAGCATCCAA ACTGTGTAAC ATCGTCCTTT ATCCCCGTCA AGCCTTTCAA CGTGACGGCT 
CAACCGGAAC CCACAGTGGA GGTGGAAGAA TTTGTTTATG ACTCAACAAA GTACTGCCGT 
CCTTACAGAG TATACAAAAA TCAAATTTAC ATTTACCCCA AGCACCTCAA GTATGATAGC 
CAGAAATGTT TCAACAAGGC ACGAAATATA ACCGTGTGCA TTGAATTCAA AAATTCTGAC 
GACGAAGGTG CCAAGCCCAT GAAGTGTATT TATGGGAAGC CTGGAGGGCC CCTCTTCACC 
TCAGCCGCCT ACACAGCAGT TCTGCACCAT TCCCAGAATC CGGATTTCTC AGACGAGGTG 
AAAATTGAGC TACCAACACA ACTCCACGAG AAACACCATA TTCTGTTTTC TTTTTATCAT 
GTCACTTGCG ACATAAATGC AAAAGCTAAT GCCAAAAAGA AGGAAGTTCT GGAAACATCA 
GTTGGATATG CTTGGCTTCC TCTAATGAAA CATGATCAGA TAGCTTCTCA AGAGTACAAT 
ATCCCAATAG CTACAAGCCT GCCTCCTAAT TATTTAAGCT TTCAAGATTC TGCAAGTGGA 
AAGCATGGTG GGAGTGACAT TAAATGGGTT GATGGTGGCA AACCACTTTT CAAAGTATCA 
ACATTCGTTG TATCAACAGT GAATACTCAG GATCCGTATG TGAACGCGTT TTTCCGACAG 
TGCCAAAAAA GAGAGAAAGA TATGTCTCAG TCACCTACCT CGAATTTCAT CCGCTCTTGT 
AAGAACTTAT TGAATGTGGA AAAAATTCAC GCAATCATGA GTTTTCTGCC TATAATCTTG 
AATCAGCTCT TCAAGGTTTT GGTACAGAAT GAGGAAGATG AAATAAGTAC AACTGTGACC 
AGGGTTCTGA CTGACATTGT GGCCAAGTGC CACGAGGAGC AGCTGGACCA CTCCGTCCAG 
TCATACATTA AGTTTGTGTT CAAGACCAAG GCTTGCAAGG AGAGAACGAT ACACGAGGAA 
CTGACTAAGA GTGTGACTGG TCTTTTGAAA TCAAATGACT CCACAACAGT CAGGCACGTT 
CTGAAGCATT CCTGGTTCTT CTTCGCAATT ATTCTAAAAT CGATGGCACA ACACTTGATT 
GATACAAATA AAATTCAGCT TTCTCGGGCT CAAAGATTTC CTGAATCTTA CCAGAATGAA 
TTGGACAATC TTATAATGGT CCTATCTGAT CACGTGATCT GGAAATACAA AGATGCCCTT 
GAAGAAACAA GAAGGGCCAA CCACAGCGTT GCCAGATTTC TCAAGCGCTG CTTCACCTTC 
ATGGATCGGG GCTTTGTGTT TAAGATGGTT AACAATTACA TCAGCATGTT CTCCTCTGGT 
GAGCTCAAGA CCTTATGCCA GTATAAATTT GATTTTCTTC AAGAAGTATG TCAGCATGAA 
CATTTTATTC CTTTGTGTCT CCCCATAAGA TCAGCAAACA TTCCAGATCC TCTGACACCT 
TCGGAATCAA CTCAAGAGCT ACATGCATCA GATATGCCTG AATATTCGGT CACGAATGAA 
TTTTGCCGCA AGCACTTCTT AATCGGGATT CTGCTCCGAG AAGTTGGCTT TGCTCTGCAG 
GAAGACCAAG ATATCAGGCA CTTAGCTTTA GCTGTCCTGA AAAATCTAAT GGCTAAGCAT 
TCGTTTGATG ATCGATACAG AGAACCAAGA AAGCAGGCCC AGATAGCAAG CTTATACATG 
CCCCTCTATG GCATGCTTCT GGACAACATG CCCAGGATTT ACCTGAAGGA TCTATATCCT 
TTCACTATCA ATACATCAAA CCAGGGGTCT AGAGATGACC TCAGCACCAA CGGAGGATTT 
CACAGCCAGT CAGCTATGAA ACATGCAAAC TCTGTGGACA CATCATTTTC CAAAGATGTT 
TTAAATTCCA TAGCAGCATT TTCATCAATA GCTATTTCTA CAGTAAACCA TGCTGATTCC 
AGAGCATCCT TAGCAAGTCT GGACTCCAAT CCAAGTACCA ATGAGAAGAG CAGTGAGAAA 
ACTGACAACT GTGAAAAGAT CCCAAGACCC TTGTCTTTGA TAGGCTCAAC GCTTCGATTT 
GACAAGTTAG ATCAAGCAGA AACCAGGAGC CTTCTTATGT GCTTTCTTCA AATTATGAAG 
ACAATTTCAG AGGAGACTCT GATTGCCTAC TGGCAAAGAG CACCTAGTCC GGAAGTGTCT 
GACTTCTTCA GCATCTTGGA TGTTTGTCTT CAAAATTTCA GATACCTAGG AAAACGCAAT 
ATAATAAGGA AAATTGCTGC TGCATTTAAA TTTGTGCAGT CCACCCAGAA CAACGGAACT 
CTCAAAGGAT CCAATCCTTC TTGCCAAACA TCGGGTCTCT TGACACAATG GATGAACACC 
ACTTCTGGTC ACGAAGGACA TAAGCAGCAC AGATCACAAA CTTTACCTAT AATTCGAGGC 
AAAAATGCAC TCTCTAACCC CAAACTCTTA CAGATGCTAG ACAATACCAT GACCAGCAAC 
TCCAATGAAA TAGACATCGT GCACCACGTG GACACAGAGG CCAATGTAGC TACAGAGGTT 
TGCCTTACTA TTCTAGACCT GCTGTCCCTC TTCACTCAGA TTCACCATAG GCAACTCCAA 
CAATCTGAAT GTCAAAATTC ATTGATGAAA AGGGTCTTTG ATACCTACAT GCTCTTCTTC 
CAAGTCAACC AGTCAGCCAC AGCACTGAAG CATGTGTTTG CTTCCCTGAG ACTGTTTGTA 
TGCAAGTTTC CCGCAGCGTT CTTTCAAGGG CCTGCTGACC TGTGTGGATC ATTCTGTTAC 
GAAGTCCTAA AATGCTGTAA CCACAGGTCA CGGTCAACCC AGACAGAAGC ATCGGCACTT 
CTATACTTTT TCATGAGGAA GAATTTTGAA TTTAATAAGC AGAAGTCAAT TGTCCGATCC 
CACCTACAGC TCATCAAGGC TGTAAGCCAG TTAATAGCCG ATGCTGGGAT TGGAGGCTCT 
CGGTTTCAAC ATTCCCTTGC AATTACCAAT AATTTTGCTA ATGGAGACAA GCAAATGAAA 
AACAGCAATT TCCCAGCAGA GGTGAAAGAT CTGACTAAAC GTATAAGGAC TGTTCTGATG 
GCCACGGCTC AGATGAAGGA GCATGAGAAG GACCCCGAGA TGCTGGTGGA CCTCCAGTAT 
AGCCTGGCAA ACTCCTATGC GAGCACCCCT GAGCTACGGA GGACTTGGCT GGAAAGCATG 
GCCAAGATTC ACGCAAGAAA TGGAGACTTG TCCGAGGCTG CCATGTGCTA CATCCACATA 
GCAGCCCTCA TCGCAGAATA CCTGAAAAGA AAGGGTTACT GGAAAATGGA AAAGATTTGC 
ACGCCATCCC TGCTCCTGGA GGATATCCAT CCCTGTGATA GCATCCCATT ACTAACAACT 
CCCAGTGGAG GAAGCATGTT CTCCATGGGC TGGCCAGCTT TTCTGAGCAT TACACCAAAT 
ATTAAAGAAG AAGGAGCAAT GAAAGAAGAT TCGGGAATGC AAGACACACC ATACAATGAG 
AATATCCTGG TGGAACAGCT GTATATGTGT GTGGAGTTTC TTTGGAAGTC TGAGAGATAT 
GAACTCATCG CTGATGTCAA CAAACCCATC ATCGCTGTCT TTGAGAAGCA GAGAGACTTC 
AAAAAATTGT CAGATCTCTA CTATGACATT CACCGGTCAT ACCTGAAAGT CGCAGAGGTG 
GTGAATTCAG AGAAGCGACT GTTTGGTCGG TACTATCGCG TGGCGTTTTA CGGGCAGGGG 
TTTTTTGAAG AAGAAGAAGG TAAAGAGTAT ATTTATAAAG AGCCTAAGCT GACAGGTCTC 
TCCGAGATTT CCCAAAGACT CCTCAAGCTG TATGCAGATA AATTCGGAGC AGACAATGTG 
AAGATAATCC AGGATTCCAA CAAGGTAAAC CCGAAGGACT TGGACCCCAA ATATGCCTAC 
ATCCAGGTGA CCTACGTCAC TCCATTCTTT GAGGAAAAGG AAATAGAGGA CCGGAAGACG 
GATTTCGAAA TGCACCACAA CATCAAGCGC TTTGTCTTTG AGACGCCCTT CACGCTGTCG 
GGCAAGAAGC ATGGTGGCGT GGAGGAGCAG TGCAAGCGAC GGACGGTGCT GACCACAAGT 
CACCTGTTCC CCTACGTGAA GAAGAGAATA CAGGTCATAA GCCAGTCGAG CACAGAACTG 
AATCCTATTG AGGTGGCGAT TGATGAGATG TCCAAGAAAG TTTCTGAGCT TAATCAGCTC 
TGCACGATGG AGGAAGTGGA CATGATCAGA CTGCAGCTCA AACTCCAAGG AAGTGTCAGC 
GTGAAGGTTA ATGCTGGACC AATGGCCTAT GCGCGAGCTT TTCTTGAAGA AACCAACGCA 
AAGAAATACC CTGACAACCA AGTAAAGCTT TTGAAGGAAA TCTTCAGGCA ATTTGCAGAT 
GCGTGTGGGC AGGCCCTGGA CGTGAATGAG CGCCTCATCA AGGAGGACCA GCTGGAGTAC 
CAGGAAGAAC TGAGGTCTCA TTATAAGGAC ATGCTCGGCG AACTCTCTGC AATTATGAAT 
GAGCAGATTA CGTACAGGGA CGACCTGGCA AAGCGCGGAT TGGAACGAAC CTTCACTCGA 
GTAACTAGCA AGGCAACTGC AGCTCCGCCC ACGGTGTCCA TCTCACCTTG CGTGGAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011362380.1
CDS	111..6653
Translation	MSFRGKVFKREPSEFWKKRRTVRRVNYEEIHRLSSQEKPRLLEPLDYETVIEELEKTYQNDPLQDLLFFPSDDFSTATVPWDIRTLYSTVPEDAEHKAENLLVKEACKFYSSQWYVVNYKYEQYSGDIRQLPRAEYKPEKLPSHSFEVDHEDADKDEDTTSHSSSKGGGGAGGTGVFKSGWLYKGNFNSTVNNTITVRSFKKRYFQLTQLPDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKYAFELKMNDLTYFVLAAETECDMEEWIHTLNRILQISPEGPLQGRRSAELTDLGLDSLDNSITCECAPEETDSSENSLHPDFAKYLTETEETVKATRNMERLNLFSMDPDIDTLKLQKKDLLESESVIKPFEEKAAKRIMIICKALTFSLQGCVTENENDPITNIEPFFVSVALYDLRNSRKISADFHVDLNHTAVRQMLSGAPVALENGNSDTVTPRQSEEPHVKGFPEEWLKFPKQAIFSVSNPHSEIVLVAKIEKVLMGNIASGAEPYIKNPDSNKYAQKILKSNRQFCSKLGKYRMPFAWAMRSVFKDNQGNVDRDSRFSPLYRHESSKISTEDLLKLVSDYRRADRISKMQTIPGSLDITVENIPLEHPNCVTSSFIPVKPFNVTAQPEPTVEVEEFVYDSTKYCRPYRVYKNQIYIYPKHLKYDSQKCFNKARNITVCIEFKNSDDEGAKPMKCIYGKPGGPLFTSAAYTAVLHHSQNPDFSDEVKIELPTQLHEKHHILFSFYHVTCDINAKANAKKKEVLETSVGYAWLPLMKHDQIASQEYNIPIATSLPPNYLSFQDSASGKHGGSDIKWVDGGKPLFKVSTFVVSTVNTQDPYVNAFFRQCQKREKDMSQSPTSNFIRSCKNLLNVEKIHAIMSFLPIILNQLFKVLVQNEEDEISTTVTRVLTDIVAKCHEEQLDHSVQSYIKFVFKTKACKERTIHEELTKSVTGLLKSNDSTTVRHVLKHSWFFFAIILKSMAQHLIDTNKIQLSRAQRFPESYQNELDNLIMVLSDHVIWKYKDALEETRRANHSVARFLKRCFTFMDRGFVFKMVNNYISMFSSGELKTLCQYKFDFLQEVCQHEHFIPLCLPIRSANIPDPLTPSESTQELHASDMPEYSVTNEFCRKHFLIGILLREVGFALQEDQDIRHLALAVLKNLMAKHSFDDRYREPRKQAQIASLYMPLYGMLLDNMPRIYLKDLYPFTINTSNQGSRDDLSTNGGFHSQSAMKHANSVDTSFSKDVLNSIAAFSSIAISTVNHADSRASLASLDSNPSTNEKSSEKTDNCEKIPRPLSLIGSTLRFDKLDQAETRSLLMCFLQIMKTISEETLIAYWQRAPSPEVSDFFSILDVCLQNFRYLGKRNIIRKIAAAFKFVQSTQNNGTLKGSNPSCQTSGLLTQWMNTTSGHEGHKQHRSQTLPIIRGKNALSNPKLLQMLDNTMTSNSNEIDIVHHVDTEANVATEVCLTILDLLSLFTQIHHRQLQQSECQNSLMKRVFDTYMLFFQVNQSATALKHVFASLRLFVCKFPAAFFQGPADLCGSFCYEVLKCCNHRSRSTQTEASALLYFFMRKNFEFNKQKSIVRSHLQLIKAVSQLIADAGIGGSRFQHSLAITNNFANGDKQMKNSNFPAEVKDLTKRIRTVLMATAQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHARNGDLSEAAMCYIHIAALIAEYLKRKGYWKMEKICTPSLLLEDIHPCDSIPLLTTPSGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQDTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVNPKDLDPKYAYIQVTYVTPFFEEKEIEDRKTDFEMHHNIKRFVFETPFTLSGKKHGGVEEQCKRRTVLTTSHLFPYVKKRIQVISQSSTELNPIEVAIDEMSKKVSELNQLCTMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEETNAKKYPDNQVKLLKEIFRQFADACGQALDVNERLIKEDQLEYQEELRSHYKDMLGELSAIMNEQITYRDDLAKRGLERTFTRVTSKATAAPPTVSISPCVEI

Target ORF information:

RefSeq Version	XM_011364078.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011364078.2

ORF Insert Sequence:

ATGAGCTTCC GAGGAAAGGT TTTTAAACGA GAGCCCAGTG AATTTTGGAA GAAGAGACGA 
ACCGTGAGGA GAGTAAATTA TGAAGAAATC CACAGGCTTA GTTCTCAGGA AAAGCCCAGG 
CTCCTTGAAC CCTTGGATTA TGAAACTGTC ATTGAAGAAC TTGAAAAGAC CTATCAGAAT 
GACCCTCTTC AAGATCTCCT GTTCTTCCCC AGTGACGACT TTTCAACGGC CACAGTTCCT 
TGGGACATTC GAACACTGTA CTCTACGGTA CCTGAAGACG CAGAGCACAA AGCAGAAAAT 
TTACTGGTCA AAGAGGCTTG TAAATTTTAT AGTTCCCAGT GGTACGTGGT AAACTACAAA 
TATGAACAAT ATTCTGGAGA TATTCGCCAG TTACCCCGAG CAGAATACAA ACCAGAGAAA 
CTCCCTTCGC ATTCCTTTGA GGTTGACCAT GAAGATGCTG ATAAGGATGA AGATACCACC 
TCCCACTCAT CTTCCAAGGG CGGTGGGGGA GCAGGGGGAA CTGGAGTCTT CAAGTCAGGC 
TGGCTCTACA AGGGGAATTT CAACAGCACC GTGAACAATA CCATCACTGT TCGGTCATTC 
AAGAAGCGCT ACTTCCAGCT GACTCAGCTG CCAGATAACT CCTACATCAT GAATTTTTAC 
AAAGATGAAA AAATATCCAA AGAGCCCAAA GGCTGCATCT TTTTGGATTC CTGTACAGGT 
GTGGTGCAGA ATAACAGACT AAGAAAATAT GCCTTTGAAT TGAAAATGAA TGACCTGACC 
TATTTTGTGC TGGCGGCGGA AACAGAGTGC GACATGGAGG AATGGATCCA CACCCTGAAC 
CGCATCCTGC AAATCAGCCC TGAGGGGCCC CTGCAGGGTC GACGGAGCGC GGAGCTCACG 
GATCTGGGCC TGGATTCACT AGATAATTCT ATCACATGTG AATGCGCACC AGAGGAAACA 
GATTCTTCAG AGAACAGCCT ACATCCAGAC TTCGCAAAAT ACCTCACAGA AACTGAAGAG 
ACTGTCAAAG CAACTCGAAA CATGGAGAGA CTCAATCTGT TCTCGATGGA CCCAGATATA 
GATACCTTGA AACTTCAGAA AAAGGATCTT TTGGAATCCG AGTCTGTGAT CAAACCATTT 
GAAGAAAAAG CTGCCAAGAG AATCATGATC ATATGTAAAG CTCTCACCTT CAGTCTTCAG 
GGATGTGTTA CAGAGAATGA AAATGATCCC ATAACCAACA TTGAGCCTTT TTTTGTGAGT 
GTGGCACTTT ATGACCTCAG AAACAGCAGG AAGATTTCTG CCGACTTCCA CGTGGATCTA 
AACCACACTG CTGTCAGGCA GATGCTCTCA GGGGCTCCCG TGGCTTTGGA AAATGGAAAC 
AGCGACACGG TCACCCCAAG ACAATCAGAA GAACCTCACG TCAAGGGGTT TCCAGAGGAA 
TGGCTGAAAT TTCCAAAGCA GGCTATATTT TCTGTAAGCA ATCCACATTC GGAAATTGTC 
TTGGTGGCTA AAATTGAAAA AGTACTAATG GGAAACATTG CAAGTGGTGC TGAACCTTAC 
ATTAAGAACC CAGACTCCAA CAAGTATGCA CAAAAGATAC TCAAATCCAA CAGACAGTTC 
TGCAGCAAGT TGGGGAAATA CCGCATGCCG TTTGCTTGGG CGATGAGATC AGTTTTTAAG 
GACAACCAGG GAAATGTGGA CAGAGACTCA AGATTTTCAC CATTGTATAG ACACGAAAGT 
AGCAAGATTT CAACTGAGGA CCTACTTAAA CTAGTGTCAG ATTATAGGAG GGCCGACAGA 
ATAAGCAAAA TGCAGACCAT TCCAGGAAGC CTCGATATCA CTGTTGAGAA CATTCCTCTG 
GAGCATCCAA ACTGTGTAAC ATCGTCCTTT ATCCCCGTCA AGCCTTTCAA CGTGACGGCT 
CAACCGGAAC CCACAGTGGA GGTGGAAGAA TTTGTTTATG ACTCAACAAA GTACTGCCGT 
CCTTACAGAG TATACAAAAA TCAAATTTAC ATTTACCCCA AGCACCTCAA GTATGATAGC 
CAGAAATGTT TCAACAAGGC ACGAAATATA ACCGTGTGCA TTGAATTCAA AAATTCTGAC 
GACGAAGGTG CCAAGCCCAT GAAGTGTATT TATGGGAAGC CTGGAGGGCC CCTCTTCACC 
TCAGCCGCCT ACACAGCAGT TCTGCACCAT TCCCAGAATC CGGATTTCTC AGACGAGGTG 
AAAATTGAGC TACCAACACA ACTCCACGAG AAACACCATA TTCTGTTTTC TTTTTATCAT 
GTCACTTGCG ACATAAATGC AAAAGCTAAT GCCAAAAAGA AGGAAGTTCT GGAAACATCA 
GTTGGATATG CTTGGCTTCC TCTAATGAAA CATGATCAGA TAGCTTCTCA AGAGTACAAT 
ATCCCAATAG CTACAAGCCT GCCTCCTAAT TATTTAAGCT TTCAAGATTC TGCAAGTGGA 
AAGCATGGTG GGAGTGACAT TAAATGGGTT GATGGTGGCA AACCACTTTT CAAAGTATCA 
ACATTCGTTG TATCAACAGT GAATACTCAG GATCCGTATG TGAACGCGTT TTTCCGACAG 
TGCCAAAAAA GAGAGAAAGA TATGTCTCAG TCACCTACCT CGAATTTCAT CCGCTCTTGT 
AAGAACTTAT TGAATGTGGA AAAAATTCAC GCAATCATGA GTTTTCTGCC TATAATCTTG 
AATCAGCTCT TCAAGGTTTT GGTACAGAAT GAGGAAGATG AAATAAGTAC AACTGTGACC 
AGGGTTCTGA CTGACATTGT GGCCAAGTGC CACGAGGAGC AGCTGGACCA CTCCGTCCAG 
TCATACATTA AGTTTGTGTT CAAGACCAAG GCTTGCAAGG AGAGAACGAT ACACGAGGAA 
CTGACTAAGA GTGTGACTGG TCTTTTGAAA TCAAATGACT CCACAACAGT CAGGCACGTT 
CTGAAGCATT CCTGGTTCTT CTTCGCAATT ATTCTAAAAT CGATGGCACA ACACTTGATT 
GATACAAATA AAATTCAGCT TTCTCGGGCT CAAAGATTTC CTGAATCTTA CCAGAATGAA 
TTGGACAATC TTATAATGGT CCTATCTGAT CACGTGATCT GGAAATACAA AGATGCCCTT 
GAAGAAACAA GAAGGGCCAA CCACAGCGTT GCCAGATTTC TCAAGCGCTG CTTCACCTTC 
ATGGATCGGG GCTTTGTGTT TAAGATGGTT AACAATTACA TCAGCATGTT CTCCTCTGGT 
GAGCTCAAGA CCTTATGCCA GTATAAATTT GATTTTCTTC AAGAAGTATG TCAGCATGAA 
CATTTTATTC CTTTGTGTCT CCCCATAAGA TCAGCAAACA TTCCAGATCC TCTGACACCT 
TCGGAATCAA CTCAAGAGCT ACATGCATCA GATATGCCTG AATATTCGGT CACGAATGAA 
TTTTGCCGCA AGCACTTCTT AATCGGGATT CTGCTCCGAG AAGTTGGCTT TGCTCTGCAG 
GAAGACCAAG ATATCAGGCA CTTAGCTTTA GCTGTCCTGA AAAATCTAAT GGCTAAGCAT 
TCGTTTGATG ATCGATACAG AGAACCAAGA AAGCAGGCCC AGATAGCAAG CTTATACATG 
CCCCTCTATG GCATGCTTCT GGACAACATG CCCAGGATTT ACCTGAAGGA TCTATATCCT 
TTCACTATCA ATACATCAAA CCAGGGGTCT AGAGATGACC TCAGCACCAA CGGAGGATTT 
CACAGCCAGT CAGCTATGAA ACATGCAAAC TCTGTGGACA CATCATTTTC CAAAGATGTT 
TTAAATTCCA TAGCAGCATT TTCATCAATA GCTATTTCTA CAGTAAACCA TGCTGATTCC 
AGAGCATCCT TAGCAAGTCT GGACTCCAAT CCAAGTACCA ATGAGAAGAG CAGTGAGAAA 
ACTGACAACT GTGAAAAGAT CCCAAGACCC TTGTCTTTGA TAGGCTCAAC GCTTCGATTT 
GACAAGTTAG ATCAAGCAGA AACCAGGAGC CTTCTTATGT GCTTTCTTCA AATTATGAAG 
ACAATTTCAG AGGAGACTCT GATTGCCTAC TGGCAAAGAG CACCTAGTCC GGAAGTGTCT 
GACTTCTTCA GCATCTTGGA TGTTTGTCTT CAAAATTTCA GATACCTAGG AAAACGCAAT 
ATAATAAGGA AAATTGCTGC TGCATTTAAA TTTGTGCAGT CCACCCAGAA CAACGGAACT 
CTCAAAGGAT CCAATCCTTC TTGCCAAACA TCGGGTCTCT TGACACAATG GATGAACACC 
ACTTCTGGTC ACGAAGGACA TAAGCAGCAC AGATCACAAA CTTTACCTAT AATTCGAGGC 
AAAAATGCAC TCTCTAACCC CAAACTCTTA CAGATGCTAG ACAATACCAT GACCAGCAAC 
TCCAATGAAA TAGACATCGT GCACCACGTG GACACAGAGG CCAATGTAGC TACAGAGGTT 
TGCCTTACTA TTCTAGACCT GCTGTCCCTC TTCACTCAGA TTCACCATAG GCAACTCCAA 
CAATCTGAAT GTCAAAATTC ATTGATGAAA AGGGTCTTTG ATACCTACAT GCTCTTCTTC 
CAAGTCAACC AGTCAGCCAC AGCACTGAAG CATGTGTTTG CTTCCCTGAG ACTGTTTGTA 
TGCAAGTTTC CCGCAGCGTT CTTTCAAGGG CCTGCTGACC TGTGTGGATC ATTCTGTTAC 
GAAGTCCTAA AATGCTGTAA CCACAGGTCA CGGTCAACCC AGACAGAAGC ATCGGCACTT 
CTATACTTTT TCATGAGGAA GAATTTTGAA TTTAATAAGC AGAAGTCAAT TGTCCGATCC 
CACCTACAGC TCATCAAGGC TGTAAGCCAG TTAATAGCCG ATGCTGGGAT TGGAGGCTCT 
CGGTTTCAAC ATTCCCTTGC AATTACCAAT AATTTTGCTA ATGGAGACAA GCAAATGAAA 
AACAGCAATT TCCCAGCAGA GGTGAAAGAT CTGACTAAAC GTATAAGGAC TGTTCTGATG 
GCCACGGCTC AGATGAAGGA GCATGAGAAG GACCCCGAGA TGCTGGTGGA CCTCCAGTAT 
AGCCTGGCAA ACTCCTATGC GAGCACCCCT GAGCTACGGA GGACTTGGCT GGAAAGCATG 
GCCAAGATTC ACGCAAGAAA TGGAGACTTG TCCGAGGCTG CCATGTGCTA CATCCACATA 
GCAGCCCTCA TCGCAGAATA CCTGAAAAGA AAGGGTTACT GGAAAATGGA AAAGATTTGC 
ACGCCATCCC TGCTCCTGGA GGATATCCAT CCCTGTGATA GCATCCCATT ACTAACAACT 
CCCAGTGGAG GAAGCATGTT CTCCATGGGC TGGCCAGCTT TTCTGAGCAT TACACCAAAT 
ATTAAAGAAG AAGGAGCAAT GAAAGAAGAT TCGGGAATGC AAGACACACC ATACAATGAG 
AATATCCTGG TGGAACAGCT GTATATGTGT GTGGAGTTTC TTTGGAAGTC TGAGAGATAT 
GAACTCATCG CTGATGTCAA CAAACCCATC ATCGCTGTCT TTGAGAAGCA GAGAGACTTC 
AAAAAATTGT CAGATCTCTA CTATGACATT CACCGGTCAT ACCTGAAAGT CGCAGAGGTG 
GTGAATTCAG AGAAGCGACT GTTTGGTCGG TACTATCGCG TGGCGTTTTA CGGGCAGGGG 
TTTTTTGAAG AAGAAGAAGG TAAAGAGTAT ATTTATAAAG AGCCTAAGCT GACAGGTCTC 
TCCGAGATTT CCCAAAGACT CCTCAAGCTG TATGCAGATA AATTCGGAGC AGACAATGTG 
AAGATAATCC AGGATTCCAA CAAGGTAAAC CCGAAGGACT TGGACCCCAA ATATGCCTAC 
ATCCAGGTGA CCTACGTCAC TCCATTCTTT GAGGAAAAGG AAATAGAGGA CCGGAAGACG 
GATTTCGAAA TGCACCACAA CATCAAGCGC TTTGTCTTTG AGACGCCCTT CACGCTGTCG 
GGCAAGAAGC ATGGTGGCGT GGAGGAGCAG TGCAAGCGAC GGACGGTGCT GACCACAAGT 
CACCTGTTCC CCTACGTGAA GAAGAGAATA CAGGTCATAA GCCAGTCGAG CACAGAACTG 
AATCCTATTG AGGTGGCGAT TGATGAGATG TCCAAGAAAG TTTCTGAGCT TAATCAGCTC 
TGCACGATGG AGGAAGTGGA CATGATCAGA CTGCAGCTCA AACTCCAAGG AAGTGTCAGC 
GTGAAGGTTA ATGCTGGACC AATGGCCTAT GCGCGAGCTT TTCTTGAAGA AACCAACGCA 
AAGAAATACC CTGACAACCA AGTAAAGCTT TTGAAGGAAA TCTTCAGGCA ATTTGCAGAT 
GCGTGTGGGC AGGCCCTGGA CGTGAATGAG CGCCTCATCA AGGAGGACCA GCTGGAGTAC 
CAGGAAGAAC TGAGGTCTCA TTATAAGGAC ATGCTCGGCG AACTCTCTGC AATTATGAAT 
GAGCAGATTA CGTACAGGGA CGACCTGGCA AAGCGCGGAT TGGAACGAAC CTTCACTCGA 
GTAACTAGCA AGGCAACTGC AGCTCCGCCC ACGGTGTCCA TCTCACCTTG CGTGGAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_011364078.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011362380.1 Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X1, mRNA.

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CloneID	OPt49583
Clone ID Related Accession (Same CDS sequence)	XM_011364080.1 , XM_011364078.2 , XM_011364078.1
Accession Version	XM_011364078.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	dedicator of cytokinesis protein 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888874.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC GAGGAAAGGT TTTTAAACGA GAGCCCAGTG AATTTTGGAA GAAGAGACGA 
ACCGTGAGGA GAGTAAATTA TGAAGAAATC CACAGGCTTA GTTCTCAGGA AAAGCCCAGG 
CTCCTTGAAC CCTTGGATTA TGAAACTGTC ATTGAAGAAC TTGAAAAGAC CTATCAGAAT 
GACCCTCTTC AAGATCTCCT GTTCTTCCCC AGTGACGACT TTTCAACGGC CACAGTTCCT 
TGGGACATTC GAACACTGTA CTCTACGGTA CCTGAAGACG CAGAGCACAA AGCAGAAAAT 
TTACTGGTCA AAGAGGCTTG TAAATTTTAT AGTTCCCAGT GGTACGTGGT AAACTACAAA 
TATGAACAAT ATTCTGGAGA TATTCGCCAG TTACCCCGAG CAGAATACAA ACCAGAGAAA 
CTCCCTTCGC ATTCCTTTGA GGTTGACCAT GAAGATGCTG ATAAGGATGA AGATACCACC 
TCCCACTCAT CTTCCAAGGG CGGTGGGGGA GCAGGGGGAA CTGGAGTCTT CAAGTCAGGC 
TGGCTCTACA AGGGGAATTT CAACAGCACC GTGAACAATA CCATCACTGT TCGGTCATTC 
AAGAAGCGCT ACTTCCAGCT GACTCAGCTG CCAGATAACT CCTACATCAT GAATTTTTAC 
AAAGATGAAA AAATATCCAA AGAGCCCAAA GGCTGCATCT TTTTGGATTC CTGTACAGGT 
GTGGTGCAGA ATAACAGACT AAGAAAATAT GCCTTTGAAT TGAAAATGAA TGACCTGACC 
TATTTTGTGC TGGCGGCGGA AACAGAGTGC GACATGGAGG AATGGATCCA CACCCTGAAC 
CGCATCCTGC AAATCAGCCC TGAGGGGCCC CTGCAGGGTC GACGGAGCGC GGAGCTCACG 
GATCTGGGCC TGGATTCACT AGATAATTCT ATCACATGTG AATGCGCACC AGAGGAAACA 
GATTCTTCAG AGAACAGCCT ACATCCAGAC TTCGCAAAAT ACCTCACAGA AACTGAAGAG 
ACTGTCAAAG CAACTCGAAA CATGGAGAGA CTCAATCTGT TCTCGATGGA CCCAGATATA 
GATACCTTGA AACTTCAGAA AAAGGATCTT TTGGAATCCG AGTCTGTGAT CAAACCATTT 
GAAGAAAAAG CTGCCAAGAG AATCATGATC ATATGTAAAG CTCTCACCTT CAGTCTTCAG 
GGATGTGTTA CAGAGAATGA AAATGATCCC ATAACCAACA TTGAGCCTTT TTTTGTGAGT 
GTGGCACTTT ATGACCTCAG AAACAGCAGG AAGATTTCTG CCGACTTCCA CGTGGATCTA 
AACCACACTG CTGTCAGGCA GATGCTCTCA GGGGCTCCCG TGGCTTTGGA AAATGGAAAC 
AGCGACACGG TCACCCCAAG ACAATCAGAA GAACCTCACG TCAAGGGGTT TCCAGAGGAA 
TGGCTGAAAT TTCCAAAGCA GGCTATATTT TCTGTAAGCA ATCCACATTC GGAAATTGTC 
TTGGTGGCTA AAATTGAAAA AGTACTAATG GGAAACATTG CAAGTGGTGC TGAACCTTAC 
ATTAAGAACC CAGACTCCAA CAAGTATGCA CAAAAGATAC TCAAATCCAA CAGACAGTTC 
TGCAGCAAGT TGGGGAAATA CCGCATGCCG TTTGCTTGGG CGATGAGATC AGTTTTTAAG 
GACAACCAGG GAAATGTGGA CAGAGACTCA AGATTTTCAC CATTGTATAG ACACGAAAGT 
AGCAAGATTT CAACTGAGGA CCTACTTAAA CTAGTGTCAG ATTATAGGAG GGCCGACAGA 
ATAAGCAAAA TGCAGACCAT TCCAGGAAGC CTCGATATCA CTGTTGAGAA CATTCCTCTG 
GAGCATCCAA ACTGTGTAAC ATCGTCCTTT ATCCCCGTCA AGCCTTTCAA CGTGACGGCT 
CAACCGGAAC CCACAGTGGA GGTGGAAGAA TTTGTTTATG ACTCAACAAA GTACTGCCGT 
CCTTACAGAG TATACAAAAA TCAAATTTAC ATTTACCCCA AGCACCTCAA GTATGATAGC 
CAGAAATGTT TCAACAAGGC ACGAAATATA ACCGTGTGCA TTGAATTCAA AAATTCTGAC 
GACGAAGGTG CCAAGCCCAT GAAGTGTATT TATGGGAAGC CTGGAGGGCC CCTCTTCACC 
TCAGCCGCCT ACACAGCAGT TCTGCACCAT TCCCAGAATC CGGATTTCTC AGACGAGGTG 
AAAATTGAGC TACCAACACA ACTCCACGAG AAACACCATA TTCTGTTTTC TTTTTATCAT 
GTCACTTGCG ACATAAATGC AAAAGCTAAT GCCAAAAAGA AGGAAGTTCT GGAAACATCA 
GTTGGATATG CTTGGCTTCC TCTAATGAAA CATGATCAGA TAGCTTCTCA AGAGTACAAT 
ATCCCAATAG CTACAAGCCT GCCTCCTAAT TATTTAAGCT TTCAAGATTC TGCAAGTGGA 
AAGCATGGTG GGAGTGACAT TAAATGGGTT GATGGTGGCA AACCACTTTT CAAAGTATCA 
ACATTCGTTG TATCAACAGT GAATACTCAG GATCCGTATG TGAACGCGTT TTTCCGACAG 
TGCCAAAAAA GAGAGAAAGA TATGTCTCAG TCACCTACCT CGAATTTCAT CCGCTCTTGT 
AAGAACTTAT TGAATGTGGA AAAAATTCAC GCAATCATGA GTTTTCTGCC TATAATCTTG 
AATCAGCTCT TCAAGGTTTT GGTACAGAAT GAGGAAGATG AAATAAGTAC AACTGTGACC 
AGGGTTCTGA CTGACATTGT GGCCAAGTGC CACGAGGAGC AGCTGGACCA CTCCGTCCAG 
TCATACATTA AGTTTGTGTT CAAGACCAAG GCTTGCAAGG AGAGAACGAT ACACGAGGAA 
CTGACTAAGA GTGTGACTGG TCTTTTGAAA TCAAATGACT CCACAACAGT CAGGCACGTT 
CTGAAGCATT CCTGGTTCTT CTTCGCAATT ATTCTAAAAT CGATGGCACA ACACTTGATT 
GATACAAATA AAATTCAGCT TTCTCGGGCT CAAAGATTTC CTGAATCTTA CCAGAATGAA 
TTGGACAATC TTATAATGGT CCTATCTGAT CACGTGATCT GGAAATACAA AGATGCCCTT 
GAAGAAACAA GAAGGGCCAA CCACAGCGTT GCCAGATTTC TCAAGCGCTG CTTCACCTTC 
ATGGATCGGG GCTTTGTGTT TAAGATGGTT AACAATTACA TCAGCATGTT CTCCTCTGGT 
GAGCTCAAGA CCTTATGCCA GTATAAATTT GATTTTCTTC AAGAAGTATG TCAGCATGAA 
CATTTTATTC CTTTGTGTCT CCCCATAAGA TCAGCAAACA TTCCAGATCC TCTGACACCT 
TCGGAATCAA CTCAAGAGCT ACATGCATCA GATATGCCTG AATATTCGGT CACGAATGAA 
TTTTGCCGCA AGCACTTCTT AATCGGGATT CTGCTCCGAG AAGTTGGCTT TGCTCTGCAG 
GAAGACCAAG ATATCAGGCA CTTAGCTTTA GCTGTCCTGA AAAATCTAAT GGCTAAGCAT 
TCGTTTGATG ATCGATACAG AGAACCAAGA AAGCAGGCCC AGATAGCAAG CTTATACATG 
CCCCTCTATG GCATGCTTCT GGACAACATG CCCAGGATTT ACCTGAAGGA TCTATATCCT 
TTCACTATCA ATACATCAAA CCAGGGGTCT AGAGATGACC TCAGCACCAA CGGAGGATTT 
CACAGCCAGT CAGCTATGAA ACATGCAAAC TCTGTGGACA CATCATTTTC CAAAGATGTT 
TTAAATTCCA TAGCAGCATT TTCATCAATA GCTATTTCTA CAGTAAACCA TGCTGATTCC 
AGAGCATCCT TAGCAAGTCT GGACTCCAAT CCAAGTACCA ATGAGAAGAG CAGTGAGAAA 
ACTGACAACT GTGAAAAGAT CCCAAGACCC TTGTCTTTGA TAGGCTCAAC GCTTCGATTT 
GACAAGTTAG ATCAAGCAGA AACCAGGAGC CTTCTTATGT GCTTTCTTCA AATTATGAAG 
ACAATTTCAG AGGAGACTCT GATTGCCTAC TGGCAAAGAG CACCTAGTCC GGAAGTGTCT 
GACTTCTTCA GCATCTTGGA TGTTTGTCTT CAAAATTTCA GATACCTAGG AAAACGCAAT 
ATAATAAGGA AAATTGCTGC TGCATTTAAA TTTGTGCAGT CCACCCAGAA CAACGGAACT 
CTCAAAGGAT CCAATCCTTC TTGCCAAACA TCGGGTCTCT TGACACAATG GATGAACACC 
ACTTCTGGTC ACGAAGGACA TAAGCAGCAC AGATCACAAA CTTTACCTAT AATTCGAGGC 
AAAAATGCAC TCTCTAACCC CAAACTCTTA CAGATGCTAG ACAATACCAT GACCAGCAAC 
TCCAATGAAA TAGACATCGT GCACCACGTG GACACAGAGG CCAATGTAGC TACAGAGGTT 
TGCCTTACTA TTCTAGACCT GCTGTCCCTC TTCACTCAGA TTCACCATAG GCAACTCCAA 
CAATCTGAAT GTCAAAATTC ATTGATGAAA AGGGTCTTTG ATACCTACAT GCTCTTCTTC 
CAAGTCAACC AGTCAGCCAC AGCACTGAAG CATGTGTTTG CTTCCCTGAG ACTGTTTGTA 
TGCAAGTTTC CCGCAGCGTT CTTTCAAGGG CCTGCTGACC TGTGTGGATC ATTCTGTTAC 
GAAGTCCTAA AATGCTGTAA CCACAGGTCA CGGTCAACCC AGACAGAAGC ATCGGCACTT 
CTATACTTTT TCATGAGGAA GAATTTTGAA TTTAATAAGC AGAAGTCAAT TGTCCGATCC 
CACCTACAGC TCATCAAGGC TGTAAGCCAG TTAATAGCCG ATGCTGGGAT TGGAGGCTCT 
CGGTTTCAAC ATTCCCTTGC AATTACCAAT AATTTTGCTA ATGGAGACAA GCAAATGAAA 
AACAGCAATT TCCCAGCAGA GGTGAAAGAT CTGACTAAAC GTATAAGGAC TGTTCTGATG 
GCCACGGCTC AGATGAAGGA GCATGAGAAG GACCCCGAGA TGCTGGTGGA CCTCCAGTAT 
AGCCTGGCAA ACTCCTATGC GAGCACCCCT GAGCTACGGA GGACTTGGCT GGAAAGCATG 
GCCAAGATTC ACGCAAGAAA TGGAGACTTG TCCGAGGCTG CCATGTGCTA CATCCACATA 
GCAGCCCTCA TCGCAGAATA CCTGAAAAGA AAGGGTTACT GGAAAATGGA AAAGATTTGC 
ACGCCATCCC TGCTCCTGGA GGATATCCAT CCCTGTGATA GCATCCCATT ACTAACAACT 
CCCAGTGGAG GAAGCATGTT CTCCATGGGC TGGCCAGCTT TTCTGAGCAT TACACCAAAT 
ATTAAAGAAG AAGGAGCAAT GAAAGAAGAT TCGGGAATGC AAGACACACC ATACAATGAG 
AATATCCTGG TGGAACAGCT GTATATGTGT GTGGAGTTTC TTTGGAAGTC TGAGAGATAT 
GAACTCATCG CTGATGTCAA CAAACCCATC ATCGCTGTCT TTGAGAAGCA GAGAGACTTC 
AAAAAATTGT CAGATCTCTA CTATGACATT CACCGGTCAT ACCTGAAAGT CGCAGAGGTG 
GTGAATTCAG AGAAGCGACT GTTTGGTCGG TACTATCGCG TGGCGTTTTA CGGGCAGGGG 
TTTTTTGAAG AAGAAGAAGG TAAAGAGTAT ATTTATAAAG AGCCTAAGCT GACAGGTCTC 
TCCGAGATTT CCCAAAGACT CCTCAAGCTG TATGCAGATA AATTCGGAGC AGACAATGTG 
AAGATAATCC AGGATTCCAA CAAGGTAAAC CCGAAGGACT TGGACCCCAA ATATGCCTAC 
ATCCAGGTGA CCTACGTCAC TCCATTCTTT GAGGAAAAGG AAATAGAGGA CCGGAAGACG 
GATTTCGAAA TGCACCACAA CATCAAGCGC TTTGTCTTTG AGACGCCCTT CACGCTGTCG 
GGCAAGAAGC ATGGTGGCGT GGAGGAGCAG TGCAAGCGAC GGACGGTGCT GACCACAAGT 
CACCTGTTCC CCTACGTGAA GAAGAGAATA CAGGTCATAA GCCAGTCGAG CACAGAACTG 
AATCCTATTG AGGTGGCGAT TGATGAGATG TCCAAGAAAG TTTCTGAGCT TAATCAGCTC 
TGCACGATGG AGGAAGTGGA CATGATCAGA CTGCAGCTCA AACTCCAAGG AAGTGTCAGC 
GTGAAGGTTA ATGCTGGACC AATGGCCTAT GCGCGAGCTT TTCTTGAAGA AACCAACGCA 
AAGAAATACC CTGACAACCA AGTAAAGCTT TTGAAGGAAA TCTTCAGGCA ATTTGCAGAT 
GCGTGTGGGC AGGCCCTGGA CGTGAATGAG CGCCTCATCA AGGAGGACCA GCTGGAGTAC 
CAGGAAGAAC TGAGGTCTCA TTATAAGGAC ATGCTCGGCG AACTCTCTGC AATTATGAAT 
GAGCAGATTA CGTACAGGGA CGACCTGGCA AAGCGCGGAT TGGAACGAAC CTTCACTCGA 
GTAACTAGCA AGGCAACTGC AGCTCCGCCC ACGGTGTCCA TCTCACCTTG CGTGGAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011362380.1
CDS	136..6678
Translation	MSFRGKVFKREPSEFWKKRRTVRRVNYEEIHRLSSQEKPRLLEPLDYETVIEELEKTYQNDPLQDLLFFPSDDFSTATVPWDIRTLYSTVPEDAEHKAENLLVKEACKFYSSQWYVVNYKYEQYSGDIRQLPRAEYKPEKLPSHSFEVDHEDADKDEDTTSHSSSKGGGGAGGTGVFKSGWLYKGNFNSTVNNTITVRSFKKRYFQLTQLPDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKYAFELKMNDLTYFVLAAETECDMEEWIHTLNRILQISPEGPLQGRRSAELTDLGLDSLDNSITCECAPEETDSSENSLHPDFAKYLTETEETVKATRNMERLNLFSMDPDIDTLKLQKKDLLESESVIKPFEEKAAKRIMIICKALTFSLQGCVTENENDPITNIEPFFVSVALYDLRNSRKISADFHVDLNHTAVRQMLSGAPVALENGNSDTVTPRQSEEPHVKGFPEEWLKFPKQAIFSVSNPHSEIVLVAKIEKVLMGNIASGAEPYIKNPDSNKYAQKILKSNRQFCSKLGKYRMPFAWAMRSVFKDNQGNVDRDSRFSPLYRHESSKISTEDLLKLVSDYRRADRISKMQTIPGSLDITVENIPLEHPNCVTSSFIPVKPFNVTAQPEPTVEVEEFVYDSTKYCRPYRVYKNQIYIYPKHLKYDSQKCFNKARNITVCIEFKNSDDEGAKPMKCIYGKPGGPLFTSAAYTAVLHHSQNPDFSDEVKIELPTQLHEKHHILFSFYHVTCDINAKANAKKKEVLETSVGYAWLPLMKHDQIASQEYNIPIATSLPPNYLSFQDSASGKHGGSDIKWVDGGKPLFKVSTFVVSTVNTQDPYVNAFFRQCQKREKDMSQSPTSNFIRSCKNLLNVEKIHAIMSFLPIILNQLFKVLVQNEEDEISTTVTRVLTDIVAKCHEEQLDHSVQSYIKFVFKTKACKERTIHEELTKSVTGLLKSNDSTTVRHVLKHSWFFFAIILKSMAQHLIDTNKIQLSRAQRFPESYQNELDNLIMVLSDHVIWKYKDALEETRRANHSVARFLKRCFTFMDRGFVFKMVNNYISMFSSGELKTLCQYKFDFLQEVCQHEHFIPLCLPIRSANIPDPLTPSESTQELHASDMPEYSVTNEFCRKHFLIGILLREVGFALQEDQDIRHLALAVLKNLMAKHSFDDRYREPRKQAQIASLYMPLYGMLLDNMPRIYLKDLYPFTINTSNQGSRDDLSTNGGFHSQSAMKHANSVDTSFSKDVLNSIAAFSSIAISTVNHADSRASLASLDSNPSTNEKSSEKTDNCEKIPRPLSLIGSTLRFDKLDQAETRSLLMCFLQIMKTISEETLIAYWQRAPSPEVSDFFSILDVCLQNFRYLGKRNIIRKIAAAFKFVQSTQNNGTLKGSNPSCQTSGLLTQWMNTTSGHEGHKQHRSQTLPIIRGKNALSNPKLLQMLDNTMTSNSNEIDIVHHVDTEANVATEVCLTILDLLSLFTQIHHRQLQQSECQNSLMKRVFDTYMLFFQVNQSATALKHVFASLRLFVCKFPAAFFQGPADLCGSFCYEVLKCCNHRSRSTQTEASALLYFFMRKNFEFNKQKSIVRSHLQLIKAVSQLIADAGIGGSRFQHSLAITNNFANGDKQMKNSNFPAEVKDLTKRIRTVLMATAQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHARNGDLSEAAMCYIHIAALIAEYLKRKGYWKMEKICTPSLLLEDIHPCDSIPLLTTPSGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQDTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVNPKDLDPKYAYIQVTYVTPFFEEKEIEDRKTDFEMHHNIKRFVFETPFTLSGKKHGGVEEQCKRRTVLTTSHLFPYVKKRIQVISQSSTELNPIEVAIDEMSKKVSELNQLCTMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEETNAKKYPDNQVKLLKEIFRQFADACGQALDVNERLIKEDQLEYQEELRSHYKDMLGELSAIMNEQITYRDDLAKRGLERTFTRVTSKATAAPPTVSISPCVEI

Target ORF information:

RefSeq Version	XM_011364078.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011364078.1

ORF Insert Sequence:

ATGAGCTTCC GAGGAAAGGT TTTTAAACGA GAGCCCAGTG AATTTTGGAA GAAGAGACGA 
ACCGTGAGGA GAGTAAATTA TGAAGAAATC CACAGGCTTA GTTCTCAGGA AAAGCCCAGG 
CTCCTTGAAC CCTTGGATTA TGAAACTGTC ATTGAAGAAC TTGAAAAGAC CTATCAGAAT 
GACCCTCTTC AAGATCTCCT GTTCTTCCCC AGTGACGACT TTTCAACGGC CACAGTTCCT 
TGGGACATTC GAACACTGTA CTCTACGGTA CCTGAAGACG CAGAGCACAA AGCAGAAAAT 
TTACTGGTCA AAGAGGCTTG TAAATTTTAT AGTTCCCAGT GGTACGTGGT AAACTACAAA 
TATGAACAAT ATTCTGGAGA TATTCGCCAG TTACCCCGAG CAGAATACAA ACCAGAGAAA 
CTCCCTTCGC ATTCCTTTGA GGTTGACCAT GAAGATGCTG ATAAGGATGA AGATACCACC 
TCCCACTCAT CTTCCAAGGG CGGTGGGGGA GCAGGGGGAA CTGGAGTCTT CAAGTCAGGC 
TGGCTCTACA AGGGGAATTT CAACAGCACC GTGAACAATA CCATCACTGT TCGGTCATTC 
AAGAAGCGCT ACTTCCAGCT GACTCAGCTG CCAGATAACT CCTACATCAT GAATTTTTAC 
AAAGATGAAA AAATATCCAA AGAGCCCAAA GGCTGCATCT TTTTGGATTC CTGTACAGGT 
GTGGTGCAGA ATAACAGACT AAGAAAATAT GCCTTTGAAT TGAAAATGAA TGACCTGACC 
TATTTTGTGC TGGCGGCGGA AACAGAGTGC GACATGGAGG AATGGATCCA CACCCTGAAC 
CGCATCCTGC AAATCAGCCC TGAGGGGCCC CTGCAGGGTC GACGGAGCGC GGAGCTCACG 
GATCTGGGCC TGGATTCACT AGATAATTCT ATCACATGTG AATGCGCACC AGAGGAAACA 
GATTCTTCAG AGAACAGCCT ACATCCAGAC TTCGCAAAAT ACCTCACAGA AACTGAAGAG 
ACTGTCAAAG CAACTCGAAA CATGGAGAGA CTCAATCTGT TCTCGATGGA CCCAGATATA 
GATACCTTGA AACTTCAGAA AAAGGATCTT TTGGAATCCG AGTCTGTGAT CAAACCATTT 
GAAGAAAAAG CTGCCAAGAG AATCATGATC ATATGTAAAG CTCTCACCTT CAGTCTTCAG 
GGATGTGTTA CAGAGAATGA AAATGATCCC ATAACCAACA TTGAGCCTTT TTTTGTGAGT 
GTGGCACTTT ATGACCTCAG AAACAGCAGG AAGATTTCTG CCGACTTCCA CGTGGATCTA 
AACCACACTG CTGTCAGGCA GATGCTCTCA GGGGCTCCCG TGGCTTTGGA AAATGGAAAC 
AGCGACACGG TCACCCCAAG ACAATCAGAA GAACCTCACG TCAAGGGGTT TCCAGAGGAA 
TGGCTGAAAT TTCCAAAGCA GGCTATATTT TCTGTAAGCA ATCCACATTC GGAAATTGTC 
TTGGTGGCTA AAATTGAAAA AGTACTAATG GGAAACATTG CAAGTGGTGC TGAACCTTAC 
ATTAAGAACC CAGACTCCAA CAAGTATGCA CAAAAGATAC TCAAATCCAA CAGACAGTTC 
TGCAGCAAGT TGGGGAAATA CCGCATGCCG TTTGCTTGGG CGATGAGATC AGTTTTTAAG 
GACAACCAGG GAAATGTGGA CAGAGACTCA AGATTTTCAC CATTGTATAG ACACGAAAGT 
AGCAAGATTT CAACTGAGGA CCTACTTAAA CTAGTGTCAG ATTATAGGAG GGCCGACAGA 
ATAAGCAAAA TGCAGACCAT TCCAGGAAGC CTCGATATCA CTGTTGAGAA CATTCCTCTG 
GAGCATCCAA ACTGTGTAAC ATCGTCCTTT ATCCCCGTCA AGCCTTTCAA CGTGACGGCT 
CAACCGGAAC CCACAGTGGA GGTGGAAGAA TTTGTTTATG ACTCAACAAA GTACTGCCGT 
CCTTACAGAG TATACAAAAA TCAAATTTAC ATTTACCCCA AGCACCTCAA GTATGATAGC 
CAGAAATGTT TCAACAAGGC ACGAAATATA ACCGTGTGCA TTGAATTCAA AAATTCTGAC 
GACGAAGGTG CCAAGCCCAT GAAGTGTATT TATGGGAAGC CTGGAGGGCC CCTCTTCACC 
TCAGCCGCCT ACACAGCAGT TCTGCACCAT TCCCAGAATC CGGATTTCTC AGACGAGGTG 
AAAATTGAGC TACCAACACA ACTCCACGAG AAACACCATA TTCTGTTTTC TTTTTATCAT 
GTCACTTGCG ACATAAATGC AAAAGCTAAT GCCAAAAAGA AGGAAGTTCT GGAAACATCA 
GTTGGATATG CTTGGCTTCC TCTAATGAAA CATGATCAGA TAGCTTCTCA AGAGTACAAT 
ATCCCAATAG CTACAAGCCT GCCTCCTAAT TATTTAAGCT TTCAAGATTC TGCAAGTGGA 
AAGCATGGTG GGAGTGACAT TAAATGGGTT GATGGTGGCA AACCACTTTT CAAAGTATCA 
ACATTCGTTG TATCAACAGT GAATACTCAG GATCCGTATG TGAACGCGTT TTTCCGACAG 
TGCCAAAAAA GAGAGAAAGA TATGTCTCAG TCACCTACCT CGAATTTCAT CCGCTCTTGT 
AAGAACTTAT TGAATGTGGA AAAAATTCAC GCAATCATGA GTTTTCTGCC TATAATCTTG 
AATCAGCTCT TCAAGGTTTT GGTACAGAAT GAGGAAGATG AAATAAGTAC AACTGTGACC 
AGGGTTCTGA CTGACATTGT GGCCAAGTGC CACGAGGAGC AGCTGGACCA CTCCGTCCAG 
TCATACATTA AGTTTGTGTT CAAGACCAAG GCTTGCAAGG AGAGAACGAT ACACGAGGAA 
CTGACTAAGA GTGTGACTGG TCTTTTGAAA TCAAATGACT CCACAACAGT CAGGCACGTT 
CTGAAGCATT CCTGGTTCTT CTTCGCAATT ATTCTAAAAT CGATGGCACA ACACTTGATT 
GATACAAATA AAATTCAGCT TTCTCGGGCT CAAAGATTTC CTGAATCTTA CCAGAATGAA 
TTGGACAATC TTATAATGGT CCTATCTGAT CACGTGATCT GGAAATACAA AGATGCCCTT 
GAAGAAACAA GAAGGGCCAA CCACAGCGTT GCCAGATTTC TCAAGCGCTG CTTCACCTTC 
ATGGATCGGG GCTTTGTGTT TAAGATGGTT AACAATTACA TCAGCATGTT CTCCTCTGGT 
GAGCTCAAGA CCTTATGCCA GTATAAATTT GATTTTCTTC AAGAAGTATG TCAGCATGAA 
CATTTTATTC CTTTGTGTCT CCCCATAAGA TCAGCAAACA TTCCAGATCC TCTGACACCT 
TCGGAATCAA CTCAAGAGCT ACATGCATCA GATATGCCTG AATATTCGGT CACGAATGAA 
TTTTGCCGCA AGCACTTCTT AATCGGGATT CTGCTCCGAG AAGTTGGCTT TGCTCTGCAG 
GAAGACCAAG ATATCAGGCA CTTAGCTTTA GCTGTCCTGA AAAATCTAAT GGCTAAGCAT 
TCGTTTGATG ATCGATACAG AGAACCAAGA AAGCAGGCCC AGATAGCAAG CTTATACATG 
CCCCTCTATG GCATGCTTCT GGACAACATG CCCAGGATTT ACCTGAAGGA TCTATATCCT 
TTCACTATCA ATACATCAAA CCAGGGGTCT AGAGATGACC TCAGCACCAA CGGAGGATTT 
CACAGCCAGT CAGCTATGAA ACATGCAAAC TCTGTGGACA CATCATTTTC CAAAGATGTT 
TTAAATTCCA TAGCAGCATT TTCATCAATA GCTATTTCTA CAGTAAACCA TGCTGATTCC 
AGAGCATCCT TAGCAAGTCT GGACTCCAAT CCAAGTACCA ATGAGAAGAG CAGTGAGAAA 
ACTGACAACT GTGAAAAGAT CCCAAGACCC TTGTCTTTGA TAGGCTCAAC GCTTCGATTT 
GACAAGTTAG ATCAAGCAGA AACCAGGAGC CTTCTTATGT GCTTTCTTCA AATTATGAAG 
ACAATTTCAG AGGAGACTCT GATTGCCTAC TGGCAAAGAG CACCTAGTCC GGAAGTGTCT 
GACTTCTTCA GCATCTTGGA TGTTTGTCTT CAAAATTTCA GATACCTAGG AAAACGCAAT 
ATAATAAGGA AAATTGCTGC TGCATTTAAA TTTGTGCAGT CCACCCAGAA CAACGGAACT 
CTCAAAGGAT CCAATCCTTC TTGCCAAACA TCGGGTCTCT TGACACAATG GATGAACACC 
ACTTCTGGTC ACGAAGGACA TAAGCAGCAC AGATCACAAA CTTTACCTAT AATTCGAGGC 
AAAAATGCAC TCTCTAACCC CAAACTCTTA CAGATGCTAG ACAATACCAT GACCAGCAAC 
TCCAATGAAA TAGACATCGT GCACCACGTG GACACAGAGG CCAATGTAGC TACAGAGGTT 
TGCCTTACTA TTCTAGACCT GCTGTCCCTC TTCACTCAGA TTCACCATAG GCAACTCCAA 
CAATCTGAAT GTCAAAATTC ATTGATGAAA AGGGTCTTTG ATACCTACAT GCTCTTCTTC 
CAAGTCAACC AGTCAGCCAC AGCACTGAAG CATGTGTTTG CTTCCCTGAG ACTGTTTGTA 
TGCAAGTTTC CCGCAGCGTT CTTTCAAGGG CCTGCTGACC TGTGTGGATC ATTCTGTTAC 
GAAGTCCTAA AATGCTGTAA CCACAGGTCA CGGTCAACCC AGACAGAAGC ATCGGCACTT 
CTATACTTTT TCATGAGGAA GAATTTTGAA TTTAATAAGC AGAAGTCAAT TGTCCGATCC 
CACCTACAGC TCATCAAGGC TGTAAGCCAG TTAATAGCCG ATGCTGGGAT TGGAGGCTCT 
CGGTTTCAAC ATTCCCTTGC AATTACCAAT AATTTTGCTA ATGGAGACAA GCAAATGAAA 
AACAGCAATT TCCCAGCAGA GGTGAAAGAT CTGACTAAAC GTATAAGGAC TGTTCTGATG 
GCCACGGCTC AGATGAAGGA GCATGAGAAG GACCCCGAGA TGCTGGTGGA CCTCCAGTAT 
AGCCTGGCAA ACTCCTATGC GAGCACCCCT GAGCTACGGA GGACTTGGCT GGAAAGCATG 
GCCAAGATTC ACGCAAGAAA TGGAGACTTG TCCGAGGCTG CCATGTGCTA CATCCACATA 
GCAGCCCTCA TCGCAGAATA CCTGAAAAGA AAGGGTTACT GGAAAATGGA AAAGATTTGC 
ACGCCATCCC TGCTCCTGGA GGATATCCAT CCCTGTGATA GCATCCCATT ACTAACAACT 
CCCAGTGGAG GAAGCATGTT CTCCATGGGC TGGCCAGCTT TTCTGAGCAT TACACCAAAT 
ATTAAAGAAG AAGGAGCAAT GAAAGAAGAT TCGGGAATGC AAGACACACC ATACAATGAG 
AATATCCTGG TGGAACAGCT GTATATGTGT GTGGAGTTTC TTTGGAAGTC TGAGAGATAT 
GAACTCATCG CTGATGTCAA CAAACCCATC ATCGCTGTCT TTGAGAAGCA GAGAGACTTC 
AAAAAATTGT CAGATCTCTA CTATGACATT CACCGGTCAT ACCTGAAAGT CGCAGAGGTG 
GTGAATTCAG AGAAGCGACT GTTTGGTCGG TACTATCGCG TGGCGTTTTA CGGGCAGGGG 
TTTTTTGAAG AAGAAGAAGG TAAAGAGTAT ATTTATAAAG AGCCTAAGCT GACAGGTCTC 
TCCGAGATTT CCCAAAGACT CCTCAAGCTG TATGCAGATA AATTCGGAGC AGACAATGTG 
AAGATAATCC AGGATTCCAA CAAGGTAAAC CCGAAGGACT TGGACCCCAA ATATGCCTAC 
ATCCAGGTGA CCTACGTCAC TCCATTCTTT GAGGAAAAGG AAATAGAGGA CCGGAAGACG 
GATTTCGAAA TGCACCACAA CATCAAGCGC TTTGTCTTTG AGACGCCCTT CACGCTGTCG 
GGCAAGAAGC ATGGTGGCGT GGAGGAGCAG TGCAAGCGAC GGACGGTGCT GACCACAAGT 
CACCTGTTCC CCTACGTGAA GAAGAGAATA CAGGTCATAA GCCAGTCGAG CACAGAACTG 
AATCCTATTG AGGTGGCGAT TGATGAGATG TCCAAGAAAG TTTCTGAGCT TAATCAGCTC 
TGCACGATGG AGGAAGTGGA CATGATCAGA CTGCAGCTCA AACTCCAAGG AAGTGTCAGC 
GTGAAGGTTA ATGCTGGACC AATGGCCTAT GCGCGAGCTT TTCTTGAAGA AACCAACGCA 
AAGAAATACC CTGACAACCA AGTAAAGCTT TTGAAGGAAA TCTTCAGGCA ATTTGCAGAT 
GCGTGTGGGC AGGCCCTGGA CGTGAATGAG CGCCTCATCA AGGAGGACCA GCTGGAGTAC 
CAGGAAGAAC TGAGGTCTCA TTATAAGGAC ATGCTCGGCG AACTCTCTGC AATTATGAAT 
GAGCAGATTA CGTACAGGGA CGACCTGGCA AAGCGCGGAT TGGAACGAAC CTTCACTCGA 
GTAACTAGCA AGGCAACTGC AGCTCCGCCC ACGGTGTCCA TCTCACCTTG CGTGGAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_011364081.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011362383.1 Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X3, mRNA.

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CloneID	OPt49584
Clone ID Related Accession (Same CDS sequence)	XM_011364081.1
Accession Version	XM_011364081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1424534400000
Organism	Pteropus vampyrus(large flying fox)
Product	dedicator of cytokinesis protein 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888874.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC GAGGAAAGGT TTTTAAACGA GAGCCCAGTG AATTTTGGAA GAAGAGACGA 
ACCGTGAGGA GAGTAAATTA TGAAGAAATC CACAGGCTTA GTTCTCAGGA AAAGCCCAGG 
CTCCTTGAAC CCTTGGATTA TGAAACTGTC ATTGAAGAAC TTGAAAAGAC CTATCAGAAT 
GACCCTCTTC AAGATCTCCT GTTCTTCCCC AGTGACGACT TTTCAACGGC CACAGTTCCT 
TGGGACATTC GAACACTGTA CTCTACGGTA CCTGAAGACG CAGAGCACAA AGCAGAAAAT 
TTACTGGTCA AAGAGGCTTG TAAATTTTAT AGTTCCCAGT GGTACGTGGT AAACTACAAA 
TATGAACAAT ATTCTGGAGA TATTCGCCAG TTACCCCGAG CAGAATACAA ACCAGAGAAA 
CTCCCTTCGC ATTCCTTTGA GGTTGACCAT GAAGATGCTG ATAAGGATGA AGATACCACC 
TCCCACTCAT CTTCCAAGGG CGGTGGGGGA GCAGGGGGAA CTGGAGTCTT CAAGTCAGGC 
TGGCTCTACA AGGGGAATTT CAACAGCACC GTGAACAATA CCATCACTGT TCGGTCATTC 
AAGAAGCGCT ACTTCCAGCT GACTCAGCTG CCAGATAACT CCTACATCAT GAATTTTTAC 
AAAGATGAAA AAATATCCAA AGAGCCCAAA GGCTGCATCT TTTTGGATTC CTGTACAGGT 
GTGGTGCAGA ATAACAGACT AAGAAAATAT GCCTTTGAAT TGAAAATGAA TGACCTGACC 
TATTTTGTGC TGGCGGCGGA AACAGAGTGC GACATGGAGG AATGGATCCA CACCCTGAAC 
CGCATCCTGC AAATCAGCCC TGAGGGGCCC CTGCAGGGTC GACGGAGCGC GGAGCTCACG 
GATCTGGGCC TGGATTCACT AGATAATTCT ATCACATGTG AATGCGCACC AGAGGAAACA 
GATTCTTCAG AGAACAGCCT ACATCCAGAC TTCGCAAAAT ACCTCACAGA AACTGAAGAG 
ACTGTCAAAG CAACTCGAAA CATGGAGAGA CTCAATCTGT TCTCGATGGA CCCAGATATA 
GATACCTTGA AACTTCAGAA AAAGGATCTT TTGGAATCCG AGTCTGTGAT CAAACCATTT 
GAAGAAAAAG CTGCCAAGAG AATCATGATC ATATGTAAAG CTCTCACCTT CAGTCTTCAG 
GGATGTGTTA CAGAGAATGA AAATGATCCC ATAACCAACA TTGAGCCTTT TTTTGTGAGT 
GTGGCACTTT ATGACCTCAG AAACAGCAGG AAGATTTCTG CCGACTTCCA CGTGGATCTA 
AACCACACTG CTGTCAGGCA GATGCTCTCA GGGGCTCCCG TGGCTTTGGA AAATGGAAAC 
AGCGACACGG TCACCCCAAG ACAATCAGAA GAACCTCACG TCAAGGGGTT TCCAGAGGAA 
TGGCTGAAAT TTCCAAAGCA GGCTATATTT TCTGTAAGCA ATCCACATTC GGAAATTGTC 
TTGGTGGCTA AAATTGAAAA AGTACTAATG GGAAACATTG CAAGTGGTGC TGAACCTTAC 
ATTAAGAACC CAGACTCCAA CAAGTATGCA CAAAAGATAC TCAAATCCAA CAGACAGTTC 
TGCAGCAAGT TGGGGAAATA CCGCATGCCG TTTGCTTGGG CGATGAGATC AGTTTTTAAG 
GACAACCAGG GAAATGTGGA CAGAGACTCA AGATTTTCAC CATTGTATAG ACACGAAAGT 
AGCAAGATTT CAACTGAGGA CCTACTTAAA CTAGTGTCAG ATTATAGGAG GGCCGACAGA 
ATAAGCAAAA TGCAGACCAT TCCAGGAAGC CTCGATATCA CTGTTGAGAA CATTCCTCTG 
GAGCATCCAA ACTGTGTAAC ATCGTCCTTT ATCCCCGTCA AGCCTTTCAA CGTGACGGCT 
CAACCGGAAC CCACAGTGGA GGTGGAAGAA TTTGTTTATG ACTCAACAAA GTACTGCCGT 
CCTTACAGAG TATACAAAAA TCAAATTTAC ATTTACCCCA AGCACCTCAA GTATGATAGC 
CAGAAATGTT TCAACAAGGC ACGAAATATA ACCGTGTGCA TTGAATTCAA AAATTCTGAC 
GACGAAGGTG CCAAGCCCAT GAAGTGTATT TATGGGAAGC CTGGAGGGCC CCTCTTCACC 
TCAGCCGCCT ACACAGCAGT TCTGCACCAT TCCCAGAATC CGGATTTCTC AGACGAGGTG 
AAAATTGAGC TACCAACACA ACTCCACGAG AAACACCATA TTCTGTTTTC TTTTTATCAT 
GTCACTTGCG ACATAAATGC AAAAGCTAAT GCCAAAAAGA AGGAAGTTCT GGAAACATCA 
GTTGGATATG CTTGGCTTCC TCTAATGAAA CATGATCAGA TAGCTTCTCA AGAGTACAAT 
ATCCCAATAG CTACAAGCCT GCCTCCTAAT TATTTAAGCT TTCAAGATTC TGCAAGTGGA 
AAGCATGGTG GGAGTGACAT TAAATGGGTT GATGGTGGCA AACCACTTTT CAAAGTATCA 
ACATTCGTTG TATCAACAGT GAATACTCAG GATCCGTATG TGAACGCGTT TTTCCGACAG 
TGCCAAAAAA GAGAGAAAGA TATGTCTCAG TCACCTACCT CGAATTTCAT CCGCTCTTGT 
AAGAACTTAT TGAATGTGGA AAAAATTCAC GCAATCATGA GTTTTCTGCC TATAATCTTG 
AATCAGCTCT TCAAGGTTTT GGTACAGAAT GAGGAAGATG AAATAAGTAC AACTGTGACC 
AGGGTTCTGA CTGACATTGT GGCCAAGTGC CACGAGGAGC AGCTGGACCA CTCCGTCCAG 
TCATACATTA AGTTTGTGTT CAAGACCAAG GCTTGCAAGG AGAGAACGAT ACACGAGGAA 
CTGACTAAGA GTGTGACTGG TCTTTTGAAA TCAAATGACT CCACAACAGT CAGGCACGTT 
CTGAAGCATT CCTGGTTCTT CTTCGCAATT ATTCTAAAAT CGATGGCACA ACACTTGATT 
GATACAAATA AAATTCAGCT TTCTCGGGCT CAAAGATTTC CTGAATCTTA CCAGAATGAA 
TTGGACAATC TTATAATGGT CCTATCTGAT CACGTGATCT GGAAATACAA AGATGCCCTT 
GAAGAAACAA GAAGGGCCAA CCACAGCGTT GCCAGATTTC TCAAGCGCTG CTTCACCTTC 
ATGGATCGGG GCTTTGTGTT TAAGATGGTT AACAATTACA TCAGCATGTT CTCCTCTGGT 
GAGCTCAAGA CCTTATGCCA GTATAAATTT GATTTTCTTC AAGAAGTATG TCAGCATGAA 
CATTTTATTC CTTTGTGTCT CCCCATAAGA TCAGCAAACA TTCCAGATCC TCTGACACCT 
TCGGAATCAA CTCAAGAGCT ACATGCATCA GATATGCCTG AATATTCGGT CACGAATGAA 
TTTTGCCGCA AGCACTTCTT AATCGGGATT CTGCTCCGAG AAGTTGGCTT TGCTCTGCAG 
GAAGACCAAG ATATCAGGCA CTTAGCTTTA GCTGTCCTGA AAAATCTAAT GGCTAAGCAT 
TCGTTTGATG ATCGATACAG AGAACCAAGA AAGCAGGCCC AGATAGCAAG CTTATACATG 
CCCCTCTATG GCATGCTTCT GGACAACATG CCCAGGATTT ACCTGAAGGA TCTATATCCT 
TTCACTATCA ATACATCAAA CCAGGGGTCT AGAGATGACC TCAGCACCAA CGGAGGATTT 
CACAGCCAGT CAGCTATGAA ACATGCAAAC TCTGTGGACA CATCATTTTC CAAAGATGTT 
TTAAATTCCA TAGCAGTAAA CCATGCTGAT TCCAGAGCAT CCTTAGCAAG TCTGGACTCC 
AATCCAAGTA CCAATGAGAA GAGCAGTGAG AAAACTGACA ACTGTGAAAA GATCCCAAGA 
CCCTTGTCTT TGATAGGCTC AACGCTTCGA TTTGACAAGT TAGATCAAGC AGAAACCAGG 
AGCCTTCTTA TGTGCTTTCT TCAAATTATG AAGACAATTT CAGAGGAGAC TCTGATTGCC 
TACTGGCAAA GAGCACCTAG TCCGGAAGTG TCTGACTTCT TCAGCATCTT GGATGTTTGT 
CTTCAAAATT TCAGATACCT AGGAAAACGC AATATAATAA GGAAAATTGC TGCTGCATTT 
AAATTTGTGC AGTCCACCCA GAACAACGGA ACTCTCAAAG GATCCAATCC TTCTTGCCAA 
ACATCGGGTC TCTTGACACA ATGGATGAAC ACCACTTCTG GTCACGAAGG ACATAAGCAG 
CACAGATCAC AAACTTTACC TATAATTCGA GGCAAAAATG CACTCTCTAA CCCCAAACTC 
TTACAGATGC TAGACAATAC CATGACCAGC AACTCCAATG AAATAGACAT CGTGCACCAC 
GTGGACACAG AGGCCAATGT AGCTACAGAG GTTTGCCTTA CTATTCTAGA CCTGCTGTCC 
CTCTTCACTC AGATTCACCA TAGGCAACTC CAACAATCTG AATGTCAAAA TTCATTGATG 
AAAAGGGTCT TTGATACCTA CATGCTCTTC TTCCAAGTCA ACCAGTCAGC CACAGCACTG 
AAGCATGTGT TTGCTTCCCT GAGACTGTTT GTATGCAAGT TTCCCGCAGC GTTCTTTCAA 
GGGCCTGCTG ACCTGTGTGG ATCATTCTGT TACGAAGTCC TAAAATGCTG TAACCACAGG 
TCACGGTCAA CCCAGACAGA AGCATCGGCA CTTCTATACT TTTTCATGAG GAAGAATTTT 
GAATTTAATA AGCAGAAGTC AATTGTCCGA TCCCACCTAC AGCTCATCAA GGCTGTAAGC 
CAGTTAATAG CCGATGCTGG GATTGGAGGC TCTCGGTTTC AACATTCCCT TGCAATTACC 
AATAATTTTG CTAATGGAGA CAAGCAAATG AAAAACAGCA ATTTCCCAGC AGAGGTGAAA 
GATCTGACTA AACGTATAAG GACTGTTCTG ATGGCCACGG CTCAGATGAA GGAGCATGAG 
AAGGACCCCG AGATGCTGGT GGACCTCCAG TATAGCCTGG CAAACTCCTA TGCGAGCACC 
CCTGAGCTAC GGAGGACTTG GCTGGAAAGC ATGGCCAAGA TTCACGCAAG AAATGGAGAC 
TTGTCCGAGG CTGCCATGTG CTACATCCAC ATAGCAGCCC TCATCGCAGA ATACCTGAAA 
AGAAAGGGTT ACTGGAAAAT GGAAAAGATT TGCACGCCAT CCCTGCTCCT GGAGGATATC 
CATCCCTGTG ATAGCATCCC ATTACTAACA ACTCCCAGTG GAGGAAGCAT GTTCTCCATG 
GGCTGGCCAG CTTTTCTGAG CATTACACCA AATATTAAAG AAGAAGGAGC AATGAAAGAA 
GATTCGGGAA TGCAAGACAC ACCATACAAT GAGAATATCC TGGTGGAACA GCTGTATATG 
TGTGTGGAGT TTCTTTGGAA GTCTGAGAGA TATGAACTCA TCGCTGATGT CAACAAACCC 
ATCATCGCTG TCTTTGAGAA GCAGAGAGAC TTCAAAAAAT TGTCAGATCT CTACTATGAC 
ATTCACCGGT CATACCTGAA AGTCGCAGAG GTGGTGAATT CAGAGAAGCG ACTGTTTGGT 
CGGTACTATC GCGTGGCGTT TTACGGGCAG GGGTTTTTTG AAGAAGAAGA AGGTAAAGAG 
TATATTTATA AAGAGCCTAA GCTGACAGGT CTCTCCGAGA TTTCCCAAAG ACTCCTCAAG 
CTGTATGCAG ATAAATTCGG AGCAGACAAT GTGAAGATAA TCCAGGATTC CAACAAGGTA 
AACCCGAAGG ACTTGGACCC CAAATATGCC TACATCCAGG TGACCTACGT CACTCCATTC 
TTTGAGGAAA AGGAAATAGA GGACCGGAAG ACGGATTTCG AAATGCACCA CAACATCAAG 
CGCTTTGTCT TTGAGACGCC CTTCACGCTG TCGGGCAAGA AGCATGGTGG CGTGGAGGAG 
CAGTGCAAGC GACGGACGGT GCTGACCACA AGTCACCTGT TCCCCTACGT GAAGAAGAGA 
ATACAGGTCA TAAGCCAGTC GAGCACAGAA CTGAATCCTA TTGAGGTGGC GATTGATGAG 
ATGTCCAAGA AAGTTTCTGA GCTTAATCAG CTCTGCACGA TGGAGGAAGT GGACATGATC 
AGACTGCAGC TCAAACTCCA AGGAAGTGTC AGCGTGAAGG TTAATGCTGG ACCAATGGCC 
TATGCGCGAG CTTTTCTTGA AGAAACCAAC GCAAAGAAAT ACCCTGACAA CCAAGTAAAG 
CTTTTGAAGG AAATCTTCAG GCAATTTGCA GATGCGTGTG GGCAGGCCCT GGACGTGAAT 
GAGCGCCTCA TCAAGGAGGA CCAGCTGGAG TACCAGGAAG AACTGAGGTC TCATTATAAG 
GACATGCTCG GCGAACTCTC TGCAATTATG AATGAGCAGA TTACGTACAG GGACGACCTG 
GCAAAGCGCG GATTGGAACG AACCTTCACT CGAGTAACTA GCAAGGCAAC TGCAGCTCCG 
CCCACGGTGT CCATCTCACC TTGCGTGGAG ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011362383.1
CDS	136..6651
Translation	MSFRGKVFKREPSEFWKKRRTVRRVNYEEIHRLSSQEKPRLLEPLDYETVIEELEKTYQNDPLQDLLFFPSDDFSTATVPWDIRTLYSTVPEDAEHKAENLLVKEACKFYSSQWYVVNYKYEQYSGDIRQLPRAEYKPEKLPSHSFEVDHEDADKDEDTTSHSSSKGGGGAGGTGVFKSGWLYKGNFNSTVNNTITVRSFKKRYFQLTQLPDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKYAFELKMNDLTYFVLAAETECDMEEWIHTLNRILQISPEGPLQGRRSAELTDLGLDSLDNSITCECAPEETDSSENSLHPDFAKYLTETEETVKATRNMERLNLFSMDPDIDTLKLQKKDLLESESVIKPFEEKAAKRIMIICKALTFSLQGCVTENENDPITNIEPFFVSVALYDLRNSRKISADFHVDLNHTAVRQMLSGAPVALENGNSDTVTPRQSEEPHVKGFPEEWLKFPKQAIFSVSNPHSEIVLVAKIEKVLMGNIASGAEPYIKNPDSNKYAQKILKSNRQFCSKLGKYRMPFAWAMRSVFKDNQGNVDRDSRFSPLYRHESSKISTEDLLKLVSDYRRADRISKMQTIPGSLDITVENIPLEHPNCVTSSFIPVKPFNVTAQPEPTVEVEEFVYDSTKYCRPYRVYKNQIYIYPKHLKYDSQKCFNKARNITVCIEFKNSDDEGAKPMKCIYGKPGGPLFTSAAYTAVLHHSQNPDFSDEVKIELPTQLHEKHHILFSFYHVTCDINAKANAKKKEVLETSVGYAWLPLMKHDQIASQEYNIPIATSLPPNYLSFQDSASGKHGGSDIKWVDGGKPLFKVSTFVVSTVNTQDPYVNAFFRQCQKREKDMSQSPTSNFIRSCKNLLNVEKIHAIMSFLPIILNQLFKVLVQNEEDEISTTVTRVLTDIVAKCHEEQLDHSVQSYIKFVFKTKACKERTIHEELTKSVTGLLKSNDSTTVRHVLKHSWFFFAIILKSMAQHLIDTNKIQLSRAQRFPESYQNELDNLIMVLSDHVIWKYKDALEETRRANHSVARFLKRCFTFMDRGFVFKMVNNYISMFSSGELKTLCQYKFDFLQEVCQHEHFIPLCLPIRSANIPDPLTPSESTQELHASDMPEYSVTNEFCRKHFLIGILLREVGFALQEDQDIRHLALAVLKNLMAKHSFDDRYREPRKQAQIASLYMPLYGMLLDNMPRIYLKDLYPFTINTSNQGSRDDLSTNGGFHSQSAMKHANSVDTSFSKDVLNSIAVNHADSRASLASLDSNPSTNEKSSEKTDNCEKIPRPLSLIGSTLRFDKLDQAETRSLLMCFLQIMKTISEETLIAYWQRAPSPEVSDFFSILDVCLQNFRYLGKRNIIRKIAAAFKFVQSTQNNGTLKGSNPSCQTSGLLTQWMNTTSGHEGHKQHRSQTLPIIRGKNALSNPKLLQMLDNTMTSNSNEIDIVHHVDTEANVATEVCLTILDLLSLFTQIHHRQLQQSECQNSLMKRVFDTYMLFFQVNQSATALKHVFASLRLFVCKFPAAFFQGPADLCGSFCYEVLKCCNHRSRSTQTEASALLYFFMRKNFEFNKQKSIVRSHLQLIKAVSQLIADAGIGGSRFQHSLAITNNFANGDKQMKNSNFPAEVKDLTKRIRTVLMATAQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHARNGDLSEAAMCYIHIAALIAEYLKRKGYWKMEKICTPSLLLEDIHPCDSIPLLTTPSGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQDTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVNPKDLDPKYAYIQVTYVTPFFEEKEIEDRKTDFEMHHNIKRFVFETPFTLSGKKHGGVEEQCKRRTVLTTSHLFPYVKKRIQVISQSSTELNPIEVAIDEMSKKVSELNQLCTMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEETNAKKYPDNQVKLLKEIFRQFADACGQALDVNERLIKEDQLEYQEELRSHYKDMLGELSAIMNEQITYRDDLAKRGLERTFTRVTSKATAAPPTVSISPCVEI

Target ORF information:

RefSeq Version	XM_011364081.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011364081.1

ORF Insert Sequence:

ATGAGCTTCC GAGGAAAGGT TTTTAAACGA GAGCCCAGTG AATTTTGGAA GAAGAGACGA 
ACCGTGAGGA GAGTAAATTA TGAAGAAATC CACAGGCTTA GTTCTCAGGA AAAGCCCAGG 
CTCCTTGAAC CCTTGGATTA TGAAACTGTC ATTGAAGAAC TTGAAAAGAC CTATCAGAAT 
GACCCTCTTC AAGATCTCCT GTTCTTCCCC AGTGACGACT TTTCAACGGC CACAGTTCCT 
TGGGACATTC GAACACTGTA CTCTACGGTA CCTGAAGACG CAGAGCACAA AGCAGAAAAT 
TTACTGGTCA AAGAGGCTTG TAAATTTTAT AGTTCCCAGT GGTACGTGGT AAACTACAAA 
TATGAACAAT ATTCTGGAGA TATTCGCCAG TTACCCCGAG CAGAATACAA ACCAGAGAAA 
CTCCCTTCGC ATTCCTTTGA GGTTGACCAT GAAGATGCTG ATAAGGATGA AGATACCACC 
TCCCACTCAT CTTCCAAGGG CGGTGGGGGA GCAGGGGGAA CTGGAGTCTT CAAGTCAGGC 
TGGCTCTACA AGGGGAATTT CAACAGCACC GTGAACAATA CCATCACTGT TCGGTCATTC 
AAGAAGCGCT ACTTCCAGCT GACTCAGCTG CCAGATAACT CCTACATCAT GAATTTTTAC 
AAAGATGAAA AAATATCCAA AGAGCCCAAA GGCTGCATCT TTTTGGATTC CTGTACAGGT 
GTGGTGCAGA ATAACAGACT AAGAAAATAT GCCTTTGAAT TGAAAATGAA TGACCTGACC 
TATTTTGTGC TGGCGGCGGA AACAGAGTGC GACATGGAGG AATGGATCCA CACCCTGAAC 
CGCATCCTGC AAATCAGCCC TGAGGGGCCC CTGCAGGGTC GACGGAGCGC GGAGCTCACG 
GATCTGGGCC TGGATTCACT AGATAATTCT ATCACATGTG AATGCGCACC AGAGGAAACA 
GATTCTTCAG AGAACAGCCT ACATCCAGAC TTCGCAAAAT ACCTCACAGA AACTGAAGAG 
ACTGTCAAAG CAACTCGAAA CATGGAGAGA CTCAATCTGT TCTCGATGGA CCCAGATATA 
GATACCTTGA AACTTCAGAA AAAGGATCTT TTGGAATCCG AGTCTGTGAT CAAACCATTT 
GAAGAAAAAG CTGCCAAGAG AATCATGATC ATATGTAAAG CTCTCACCTT CAGTCTTCAG 
GGATGTGTTA CAGAGAATGA AAATGATCCC ATAACCAACA TTGAGCCTTT TTTTGTGAGT 
GTGGCACTTT ATGACCTCAG AAACAGCAGG AAGATTTCTG CCGACTTCCA CGTGGATCTA 
AACCACACTG CTGTCAGGCA GATGCTCTCA GGGGCTCCCG TGGCTTTGGA AAATGGAAAC 
AGCGACACGG TCACCCCAAG ACAATCAGAA GAACCTCACG TCAAGGGGTT TCCAGAGGAA 
TGGCTGAAAT TTCCAAAGCA GGCTATATTT TCTGTAAGCA ATCCACATTC GGAAATTGTC 
TTGGTGGCTA AAATTGAAAA AGTACTAATG GGAAACATTG CAAGTGGTGC TGAACCTTAC 
ATTAAGAACC CAGACTCCAA CAAGTATGCA CAAAAGATAC TCAAATCCAA CAGACAGTTC 
TGCAGCAAGT TGGGGAAATA CCGCATGCCG TTTGCTTGGG CGATGAGATC AGTTTTTAAG 
GACAACCAGG GAAATGTGGA CAGAGACTCA AGATTTTCAC CATTGTATAG ACACGAAAGT 
AGCAAGATTT CAACTGAGGA CCTACTTAAA CTAGTGTCAG ATTATAGGAG GGCCGACAGA 
ATAAGCAAAA TGCAGACCAT TCCAGGAAGC CTCGATATCA CTGTTGAGAA CATTCCTCTG 
GAGCATCCAA ACTGTGTAAC ATCGTCCTTT ATCCCCGTCA AGCCTTTCAA CGTGACGGCT 
CAACCGGAAC CCACAGTGGA GGTGGAAGAA TTTGTTTATG ACTCAACAAA GTACTGCCGT 
CCTTACAGAG TATACAAAAA TCAAATTTAC ATTTACCCCA AGCACCTCAA GTATGATAGC 
CAGAAATGTT TCAACAAGGC ACGAAATATA ACCGTGTGCA TTGAATTCAA AAATTCTGAC 
GACGAAGGTG CCAAGCCCAT GAAGTGTATT TATGGGAAGC CTGGAGGGCC CCTCTTCACC 
TCAGCCGCCT ACACAGCAGT TCTGCACCAT TCCCAGAATC CGGATTTCTC AGACGAGGTG 
AAAATTGAGC TACCAACACA ACTCCACGAG AAACACCATA TTCTGTTTTC TTTTTATCAT 
GTCACTTGCG ACATAAATGC AAAAGCTAAT GCCAAAAAGA AGGAAGTTCT GGAAACATCA 
GTTGGATATG CTTGGCTTCC TCTAATGAAA CATGATCAGA TAGCTTCTCA AGAGTACAAT 
ATCCCAATAG CTACAAGCCT GCCTCCTAAT TATTTAAGCT TTCAAGATTC TGCAAGTGGA 
AAGCATGGTG GGAGTGACAT TAAATGGGTT GATGGTGGCA AACCACTTTT CAAAGTATCA 
ACATTCGTTG TATCAACAGT GAATACTCAG GATCCGTATG TGAACGCGTT TTTCCGACAG 
TGCCAAAAAA GAGAGAAAGA TATGTCTCAG TCACCTACCT CGAATTTCAT CCGCTCTTGT 
AAGAACTTAT TGAATGTGGA AAAAATTCAC GCAATCATGA GTTTTCTGCC TATAATCTTG 
AATCAGCTCT TCAAGGTTTT GGTACAGAAT GAGGAAGATG AAATAAGTAC AACTGTGACC 
AGGGTTCTGA CTGACATTGT GGCCAAGTGC CACGAGGAGC AGCTGGACCA CTCCGTCCAG 
TCATACATTA AGTTTGTGTT CAAGACCAAG GCTTGCAAGG AGAGAACGAT ACACGAGGAA 
CTGACTAAGA GTGTGACTGG TCTTTTGAAA TCAAATGACT CCACAACAGT CAGGCACGTT 
CTGAAGCATT CCTGGTTCTT CTTCGCAATT ATTCTAAAAT CGATGGCACA ACACTTGATT 
GATACAAATA AAATTCAGCT TTCTCGGGCT CAAAGATTTC CTGAATCTTA CCAGAATGAA 
TTGGACAATC TTATAATGGT CCTATCTGAT CACGTGATCT GGAAATACAA AGATGCCCTT 
GAAGAAACAA GAAGGGCCAA CCACAGCGTT GCCAGATTTC TCAAGCGCTG CTTCACCTTC 
ATGGATCGGG GCTTTGTGTT TAAGATGGTT AACAATTACA TCAGCATGTT CTCCTCTGGT 
GAGCTCAAGA CCTTATGCCA GTATAAATTT GATTTTCTTC AAGAAGTATG TCAGCATGAA 
CATTTTATTC CTTTGTGTCT CCCCATAAGA TCAGCAAACA TTCCAGATCC TCTGACACCT 
TCGGAATCAA CTCAAGAGCT ACATGCATCA GATATGCCTG AATATTCGGT CACGAATGAA 
TTTTGCCGCA AGCACTTCTT AATCGGGATT CTGCTCCGAG AAGTTGGCTT TGCTCTGCAG 
GAAGACCAAG ATATCAGGCA CTTAGCTTTA GCTGTCCTGA AAAATCTAAT GGCTAAGCAT 
TCGTTTGATG ATCGATACAG AGAACCAAGA AAGCAGGCCC AGATAGCAAG CTTATACATG 
CCCCTCTATG GCATGCTTCT GGACAACATG CCCAGGATTT ACCTGAAGGA TCTATATCCT 
TTCACTATCA ATACATCAAA CCAGGGGTCT AGAGATGACC TCAGCACCAA CGGAGGATTT 
CACAGCCAGT CAGCTATGAA ACATGCAAAC TCTGTGGACA CATCATTTTC CAAAGATGTT 
TTAAATTCCA TAGCAGTAAA CCATGCTGAT TCCAGAGCAT CCTTAGCAAG TCTGGACTCC 
AATCCAAGTA CCAATGAGAA GAGCAGTGAG AAAACTGACA ACTGTGAAAA GATCCCAAGA 
CCCTTGTCTT TGATAGGCTC AACGCTTCGA TTTGACAAGT TAGATCAAGC AGAAACCAGG 
AGCCTTCTTA TGTGCTTTCT TCAAATTATG AAGACAATTT CAGAGGAGAC TCTGATTGCC 
TACTGGCAAA GAGCACCTAG TCCGGAAGTG TCTGACTTCT TCAGCATCTT GGATGTTTGT 
CTTCAAAATT TCAGATACCT AGGAAAACGC AATATAATAA GGAAAATTGC TGCTGCATTT 
AAATTTGTGC AGTCCACCCA GAACAACGGA ACTCTCAAAG GATCCAATCC TTCTTGCCAA 
ACATCGGGTC TCTTGACACA ATGGATGAAC ACCACTTCTG GTCACGAAGG ACATAAGCAG 
CACAGATCAC AAACTTTACC TATAATTCGA GGCAAAAATG CACTCTCTAA CCCCAAACTC 
TTACAGATGC TAGACAATAC CATGACCAGC AACTCCAATG AAATAGACAT CGTGCACCAC 
GTGGACACAG AGGCCAATGT AGCTACAGAG GTTTGCCTTA CTATTCTAGA CCTGCTGTCC 
CTCTTCACTC AGATTCACCA TAGGCAACTC CAACAATCTG AATGTCAAAA TTCATTGATG 
AAAAGGGTCT TTGATACCTA CATGCTCTTC TTCCAAGTCA ACCAGTCAGC CACAGCACTG 
AAGCATGTGT TTGCTTCCCT GAGACTGTTT GTATGCAAGT TTCCCGCAGC GTTCTTTCAA 
GGGCCTGCTG ACCTGTGTGG ATCATTCTGT TACGAAGTCC TAAAATGCTG TAACCACAGG 
TCACGGTCAA CCCAGACAGA AGCATCGGCA CTTCTATACT TTTTCATGAG GAAGAATTTT 
GAATTTAATA AGCAGAAGTC AATTGTCCGA TCCCACCTAC AGCTCATCAA GGCTGTAAGC 
CAGTTAATAG CCGATGCTGG GATTGGAGGC TCTCGGTTTC AACATTCCCT TGCAATTACC 
AATAATTTTG CTAATGGAGA CAAGCAAATG AAAAACAGCA ATTTCCCAGC AGAGGTGAAA 
GATCTGACTA AACGTATAAG GACTGTTCTG ATGGCCACGG CTCAGATGAA GGAGCATGAG 
AAGGACCCCG AGATGCTGGT GGACCTCCAG TATAGCCTGG CAAACTCCTA TGCGAGCACC 
CCTGAGCTAC GGAGGACTTG GCTGGAAAGC ATGGCCAAGA TTCACGCAAG AAATGGAGAC 
TTGTCCGAGG CTGCCATGTG CTACATCCAC ATAGCAGCCC TCATCGCAGA ATACCTGAAA 
AGAAAGGGTT ACTGGAAAAT GGAAAAGATT TGCACGCCAT CCCTGCTCCT GGAGGATATC 
CATCCCTGTG ATAGCATCCC ATTACTAACA ACTCCCAGTG GAGGAAGCAT GTTCTCCATG 
GGCTGGCCAG CTTTTCTGAG CATTACACCA AATATTAAAG AAGAAGGAGC AATGAAAGAA 
GATTCGGGAA TGCAAGACAC ACCATACAAT GAGAATATCC TGGTGGAACA GCTGTATATG 
TGTGTGGAGT TTCTTTGGAA GTCTGAGAGA TATGAACTCA TCGCTGATGT CAACAAACCC 
ATCATCGCTG TCTTTGAGAA GCAGAGAGAC TTCAAAAAAT TGTCAGATCT CTACTATGAC 
ATTCACCGGT CATACCTGAA AGTCGCAGAG GTGGTGAATT CAGAGAAGCG ACTGTTTGGT 
CGGTACTATC GCGTGGCGTT TTACGGGCAG GGGTTTTTTG AAGAAGAAGA AGGTAAAGAG 
TATATTTATA AAGAGCCTAA GCTGACAGGT CTCTCCGAGA TTTCCCAAAG ACTCCTCAAG 
CTGTATGCAG ATAAATTCGG AGCAGACAAT GTGAAGATAA TCCAGGATTC CAACAAGGTA 
AACCCGAAGG ACTTGGACCC CAAATATGCC TACATCCAGG TGACCTACGT CACTCCATTC 
TTTGAGGAAA AGGAAATAGA GGACCGGAAG ACGGATTTCG AAATGCACCA CAACATCAAG 
CGCTTTGTCT TTGAGACGCC CTTCACGCTG TCGGGCAAGA AGCATGGTGG CGTGGAGGAG 
CAGTGCAAGC GACGGACGGT GCTGACCACA AGTCACCTGT TCCCCTACGT GAAGAAGAGA 
ATACAGGTCA TAAGCCAGTC GAGCACAGAA CTGAATCCTA TTGAGGTGGC GATTGATGAG 
ATGTCCAAGA AAGTTTCTGA GCTTAATCAG CTCTGCACGA TGGAGGAAGT GGACATGATC 
AGACTGCAGC TCAAACTCCA AGGAAGTGTC AGCGTGAAGG TTAATGCTGG ACCAATGGCC 
TATGCGCGAG CTTTTCTTGA AGAAACCAAC GCAAAGAAAT ACCCTGACAA CCAAGTAAAG 
CTTTTGAAGG AAATCTTCAG GCAATTTGCA GATGCGTGTG GGCAGGCCCT GGACGTGAAT 
GAGCGCCTCA TCAAGGAGGA CCAGCTGGAG TACCAGGAAG AACTGAGGTC TCATTATAAG 
GACATGCTCG GCGAACTCTC TGCAATTATG AATGAGCAGA TTACGTACAG GGACGACCTG 
GCAAAGCGCG GATTGGAACG AACCTTCACT CGAGTAACTA GCAAGGCAAC TGCAGCTCCG 
CCCACGGTGT CCATCTCACC TTGCGTGGAG ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_023532929.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023388697.1 Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X1, mRNA.

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CloneID	OPt70924
Clone ID Related Accession (Same CDS sequence)	XM_023532929.1 , XM_023532930.1
Accession Version	XM_023532929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	dedicator of cytokinesis protein 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888874.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCA CCGCCAGCAT TGTTCTGCTA CAAGCTTTTC GTTCTGTGGT CCAGAGGAGC 
TGTCCACACA TTTGCAGCCG ACGCCAGGAG GAACCATCCC ATGAAATTTT GCCTCTAGAA 
AGTGACGATG AAATGAGCAG ACCCAGAACC CTCATCATAA TGCAGGAAAA GCCCAGGCTC 
CTTGAACCCT TGGATTATGA AACTGTCATT GAAGAACTTG AAAAGACCTA TCAGAATGAC 
CCTCTTCAAG ATCTCCTGTT CTTCCCCAGT GACGACTTTT CAACGGCCAC AGTTCCTTGG 
GACATTCGAA CACTGTACTC TACGGTACCT GAAGACGCAG AGCACAAAGC AGAAAATTTA 
CTGGTCAAAG AGGCTTGTAA ATTTTATAGT TCCCAGTGGT ACGTGGTAAA CTACAAATAT 
GAACAATATT CTGGAGATAT TCGCCAGTTA CCCCGAGCAG AATACAAACC AGAGAAACTC 
CCTTCGCATT CCTTTGAGGT TGACCATGAA GATGCTGATA AGGATGAAGA TACCACCTCC 
CACTCATCTT CCAAGGGCGG TGGGGGAGCA GGGGGAACTG GAGTCTTCAA GTCAGGCTGG 
CTCTACAAGG GGAATTTCAA CAGCACCGTG AACAATACCA TCACTGTTCG GTCATTCAAG 
AAGCGCTACT TCCAGCTGAC TCAGCTGCCA GATAACTCCT ACATCATGAA TTTTTACAAA 
GATGAAAAAA TATCCAAAGA GCCCAAAGGC TGCATCTTTT TGGATTCCTG TACAGGTGTG 
GTGCAGAATA ACAGACTAAG AAAATATGCC TTTGAATTGA AAATGAATGA CCTGACCTAT 
TTTGTGCTGG CGGCGGAAAC AGAGTGCGAC ATGGAGGAAT GGATCCACAC CCTGAACCGC 
ATCCTGCAAA TCAGCCCTGA GGGGCCCCTG CAGGGTCGAC GGAGCGCGGA GCTCACGGAT 
CTGGGCCTGG ATTCACTAGA TAATTCTATC ACATGTGAAT GCGCACCAGA GGAAACAGAT 
TCTTCAGAGA ACAGCCTACA TCCAGACTTC GCAAAATACC TCACAGAAAC TGAAGAGACT 
GTCAAAGCAA CTCGAAACAT GGAGAGACTC AATCTGTTCT CGATGGACCC AGATATAGAT 
ACCTTGAAAC TTCAGAAAAA GGATCTTTTG GAATCCGAGT CTGTGATCAA ACCATTTGAA 
GAAAAAGCTG CCAAGAGAAT CATGATCATA TGTAAAGCTC TCACCTTCAG TCTTCAGGGA 
TGTGTTACAG AGAATGAAAA TGATCCCATA ACCAACATTG AGCCTTTTTT TGTGAGTGTG 
GCACTTTATG ACCTCAGAAA CAGCAGGAAG ATTTCTGCCG ACTTCCACGT GGATCTAAAC 
CACACTGCTG TCAGGCAGAT GCTCTCAGGG GCTCCCGTGG CTTTGGAAAA TGGAAACAGC 
GACACGGTCA CCCCAAGACA ATCAGAAGAA CCTCACGTCA AGGGGTTTCC AGAGGAATGG 
CTGAAATTTC CAAAGCAGGC TATATTTTCT GTAAGCAATC CACATTCGGA AATTGTCTTG 
GTGGCTAAAA TTGAAAAAGT ACTAATGGGA AACATTGCAA GTGGTGCTGA ACCTTACATT 
AAGAACCCAG ACTCCAACAA GTATGCACAA AAGATACTCA AATCCAACAG ACAGTTCTGC 
AGCAAGTTGG GGAAATACCG CATGCCGTTT GCTTGGGCGA TGAGATCAGT TTTTAAGGAC 
AACCAGGGAA ATGTGGACAG AGACTCAAGA TTTTCACCAT TGTATAGACA CGAAAGTAGC 
AAGATTTCAA CTGAGGACCT ACTTAAACTA GTGTCAGATT ATAGGAGGGC CGACAGAATA 
AGCAAAATGC AGACCATTCC AGGAAGCCTC GATATCACTG TTGAGAACAT TCCTCTGGAG 
CATCCAAACT GTGTAACATC GTCCTTTATC CCCGTCAAGC CTTTCAACGT GACGGCTCAA 
CCGGAACCCA CAGTGGAGGT GGAAGAATTT GTTTATGACT CAACAAAGTA CTGCCGTCCT 
TACAGAGTAT ACAAAAATCA AATTTACATT TACCCCAAGC ACCTCAAGTA TGATAGCCAG 
AAATGTTTCA ACAAGGCACG AAATATAACC GTGTGCATTG AATTCAAAAA TTCTGACGAC 
GAAGGTGCCA AGCCCATGAA GTGTATTTAT GGGAAGCCTG GAGGGCCCCT CTTCACCTCA 
GCCGCCTACA CAGCAGTTCT GCACCATTCC CAGAATCCGG ATTTCTCAGA CGAGGTGAAA 
ATTGAGCTAC CAACACAACT CCACGAGAAA CACCATATTC TGTTTTCTTT TTATCATGTC 
ACTTGCGACA TAAATGCAAA AGCTAATGCC AAAAAGAAGG AAGTTCTGGA AACATCAGTT 
GGATATGCTT GGCTTCCTCT AATGAAACAT GATCAGATAG CTTCTCAAGA GTACAATATC 
CCAATAGCTA CAAGCCTGCC TCCTAATTAT TTAAGCTTTC AAGATTCTGC AAGTGGAAAG 
CATGGTGGGA GTGACATTAA ATGGGTTGAT GGTGGCAAAC CACTTTTCAA AGTATCAACA 
TTCGTTGTAT CAACAGTGAA TACTCAGGAT CCGTATGTGA ACGCGTTTTT CCGACAGTGC 
CAAAAAAGAG AGAAAGATAT GTCTCAGTCA CCTACCTCGA ATTTCATCCG CTCTTGTAAG 
AACTTATTGA ATGTGGAAAA AATTCACGCA ATCATGAGTT TTCTGCCTAT AATCTTGAAT 
CAGCTCTTCA AGGTTTTGGT ACAGAATGAG GAAGATGAAA TAAGTACAAC TGTGACCAGG 
GTTCTGACTG ACATTGTGGC CAAGTGCCAC GAGGAGCAGC TGGACCACTC CGTCCAGTCA 
TACATTAAGT TTGTGTTCAA GACCAAGGCT TGCAAGGAGA GAACGATACA CGAGGAACTG 
ACTAAGAGTG TGACTGGTCT TTTGAAATCA AATGACTCCA CAACAGTCAG GCACGTTCTG 
AAGCATTCCT GGTTCTTCTT CGCAATTATT CTAAAATCGA TGGCACAACA CTTGATTGAT 
ACAAATAAAA TTCAGCTTTC TCGGGCTCAA AGATTTCCTG AATCTTACCA GAATGAATTG 
GACAATCTTA TAATGGTCCT ATCTGATCAC GTGATCTGGA AATACAAAGA TGCCCTTGAA 
GAAACAAGAA GGGCCAACCA CAGCGTTGCC AGATTTCTCA AGCGCTGCTT CACCTTCATG 
GATCGGGGCT TTGTGTTTAA GATGGTTAAC AATTACATCA GCATGTTCTC CTCTGGTGAG 
CTCAAGACCT TATGCCAGTA TAAATTTGAT TTTCTTCAAG AAGTATGTCA GCATGAACAT 
TTTATTCCTT TGTGTCTCCC CATAAGATCA GCAAACATTC CAGATCCTCT GACACCTTCG 
GAATCAACTC AAGAGCTACA TGCATCAGAT ATGCCTGAAT ATTCGGTCAC GAATGAATTT 
TGCCGCAAGC ACTTCTTAAT CGGGATTCTG CTCCGAGAAG TTGGCTTTGC TCTGCAGGAA 
GACCAAGATA TCAGGCACTT AGCTTTAGCT GTCCTGAAAA ATCTAATGGC TAAGCATTCG 
TTTGATGATC GATACAGAGA ACCAAGAAAG CAGGCCCAGA TAGCAAGCTT ATACATGCCC 
CTCTATGGCA TGCTTCTGGA CAACATGCCC AGGATTTACC TGAAGGATCT ATATCCTTTC 
ACTATCAATA CATCAAACCA GGGGTCTAGA GATGACCTCA GCACCAACGG AGGATTTCAC 
AGCCAGTCAG CTATGAAACA TGCAAACTCT GTGGACACAT CATTTTCCAA AGATGTTTTA 
AATTCCATAG CAGCATTTTC ATCAATAGCT ATTTCTACAG TAAACCATGC TGATTCCAGA 
GCATCCTTAG CAAGTCTGGA CTCCAATCCA AGTACCAATG AGAAGAGCAG TGAGAAAACT 
GACAACTGTG AAAAGATCCC AAGACCCTTG TCTTTGATAG GCTCAACGCT TCGATTTGAC 
AAGTTAGATC AAGCAGAAAC CAGGAGCCTT CTTATGTGCT TTCTTCAAAT TATGAAGACA 
ATTTCAGAGG AGACTCTGAT TGCCTACTGG CAAAGAGCAC CTAGTCCGGA AGTGTCTGAC 
TTCTTCAGCA TCTTGGATGT TTGTCTTCAA AATTTCAGAT ACCTAGGAAA ACGCAATATA 
ATAAGGAAAA TTGCTGCTGC ATTTAAATTT GTGCAGTCCA CCCAGAACAA CGGAACTCTC 
AAAGGATCCA ATCCTTCTTG CCAAACATCG GGTCTCTTGA CACAATGGAT GAACACCACT 
TCTGGTCACG AAGGACATAA GCAGCACAGA TCACAAACTT TACCTATAAT TCGAGGCAAA 
AATGCACTCT CTAACCCCAA ACTCTTACAG ATGCTAGACA ATACCATGAC CAGCAACTCC 
AATGAAATAG ACATCGTGCA CCACGTGGAC ACAGAGGCCA ATGTAGCTAC AGAGGTTTGC 
CTTACTATTC TAGACCTGCT GTCCCTCTTC ACTCAGATTC ACCATAGGCA ACTCCAACAA 
TCTGAATGTC AAAATTCATT GATGAAAAGG GTCTTTGATA CCTACATGCT CTTCTTCCAA 
GTCAACCAGT CAGCCACAGC ACTGAAGCAT GTGTTTGCTT CCCTGAGACT GTTTGTATGC 
AAGTTTCCCG CAGCGTTCTT TCAAGGGCCT GCTGACCTGT GTGGATCATT CTGTTACGAA 
GTCCTAAAAT GCTGTAACCA CAGGTCACGG TCAACCCAGA CAGAAGCATC GGCACTTCTA 
TACTTTTTCA TGAGGAAGAA TTTTGAATTT AATAAGCAGA AGTCAATTGT CCGATCCCAC 
CTACAGCTCA TCAAGGCTGT AAGCCAGTTA ATAGCCGATG CTGGGATTGG AGGCTCTCGG 
TTTCAACATT CCCTTGCAAT TACCAATAAT TTTGCTAATG GAGACAAGCA AATGAAAAAC 
AGCAATTTCC CAGCAGAGGT GAAAGATCTG ACTAAACGTA TAAGGACTGT TCTGATGGCC 
ACGGCTCAGA TGAAGGAGCA TGAGAAGGAC CCCGAGATGC TGGTGGACCT CCAGTATAGC 
CTGGCAAACT CCTATGCGAG CACCCCTGAG CTACGGAGGA CTTGGCTGGA AAGCATGGCC 
AAGATTCACG CAAGAAATGG AGACTTGTCC GAGGCTGCCA TGTGCTACAT CCACATAGCA 
GCCCTCATCG CAGAATACCT GAAAAGAAAG GGTTACTGGA AAATGGAAAA GATTTGCACG 
CCATCCCTGC TCCTGGAGGA TATCCATCCC TGTGATAGCA TCCCATTACT AACAACTCCC 
AGTGGAGGAA GCATGTTCTC CATGGGCTGG CCAGCTTTTC TGAGCATTAC ACCAAATATT 
AAAGAAGAAG GAGCAATGAA AGAAGATTCG GGAATGCAAG ACACACCATA CAATGAGAAT 
ATCCTGGTGG AACAGCTGTA TATGTGTGTG GAGTTTCTTT GGAAGTCTGA GAGATATGAA 
CTCATCGCTG ATGTCAACAA ACCCATCATC GCTGTCTTTG AGAAGCAGAG AGACTTCAAA 
AAATTGTCAG ATCTCTACTA TGACATTCAC CGGTCATACC TGAAAGTCGC AGAGGTGGTG 
AATTCAGAGA AGCGACTGTT TGGTCGGTAC TATCGCGTGG CGTTTTACGG GCAGGGGTTT 
TTTGAAGAAG AAGAAGGTAA AGAGTATATT TATAAAGAGC CTAAGCTGAC AGGTCTCTCC 
GAGATTTCCC AAAGACTCCT CAAGCTGTAT GCAGATAAAT TCGGAGCAGA CAATGTGAAG 
ATAATCCAGG ATTCCAACAA GGTAAACCCG AAGGACTTGG ACCCCAAATA TGCCTACATC 
CAGGTGACCT ACGTCACTCC ATTCTTTGAG GAAAAGGAAA TAGAGGACCG GAAGACGGAT 
TTCGAAATGC ACCACAACAT CAAGCGCTTT GTCTTTGAGA CGCCCTTCAC GCTGTCGGGC 
AAGAAGCATG GTGGCGTGGA GGAGCAGTGC AAGCGACGGA CGGTGCTGAC CACAAGTCAC 
CTGTTCCCCT ACGTGAAGAA GAGAATACAG GTCATAAGCC AGTCGAGCAC AGAACTGAAT 
CCTATTGAGG TGGCGATTGA TGAGATGTCC AAGAAAGTTT CTGAGCTTAA TCAGCTCTGC 
ACGATGGAGG AAGTGGACAT GATCAGACTG CAGCTCAAAC TCCAAGGAAG TGTCAGCGTG 
AAGGTTAATG CTGGACCAAT GGCCTATGCG CGAGCTTTTC TTGAAGAAAC CAACGCAAAG 
AAATACCCTG ACAACCAAGT AAAGCTTTTG AAGGAAATCT TCAGGCAATT TGCAGATGCG 
TGTGGGCAGG CCCTGGACGT GAATGAGCGC CTCATCAAGG AGGACCAGCT GGAGTACCAG 
GAAGAACTGA GGTCTCATTA TAAGGACATG CTCGGCGAAC TCTCTGCAAT TATGAATGAG 
CAGATTACGT ACAGGGACGA CCTGGCAAAG CGCGGATTGG AACGAACCTT CACTCGAGTA 
ACTAGCAAGG CAACTGCAGC TCCGCCCACG GTGTCCATCT CACCTTGCGT GGAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023388697.1
CDS	16..6615
Translation	MGCTASIVLLQAFRSVVQRSCPHICSRRQEEPSHEILPLESDDEMSRPRTLIIMQEKPRLLEPLDYETVIEELEKTYQNDPLQDLLFFPSDDFSTATVPWDIRTLYSTVPEDAEHKAENLLVKEACKFYSSQWYVVNYKYEQYSGDIRQLPRAEYKPEKLPSHSFEVDHEDADKDEDTTSHSSSKGGGGAGGTGVFKSGWLYKGNFNSTVNNTITVRSFKKRYFQLTQLPDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKYAFELKMNDLTYFVLAAETECDMEEWIHTLNRILQISPEGPLQGRRSAELTDLGLDSLDNSITCECAPEETDSSENSLHPDFAKYLTETEETVKATRNMERLNLFSMDPDIDTLKLQKKDLLESESVIKPFEEKAAKRIMIICKALTFSLQGCVTENENDPITNIEPFFVSVALYDLRNSRKISADFHVDLNHTAVRQMLSGAPVALENGNSDTVTPRQSEEPHVKGFPEEWLKFPKQAIFSVSNPHSEIVLVAKIEKVLMGNIASGAEPYIKNPDSNKYAQKILKSNRQFCSKLGKYRMPFAWAMRSVFKDNQGNVDRDSRFSPLYRHESSKISTEDLLKLVSDYRRADRISKMQTIPGSLDITVENIPLEHPNCVTSSFIPVKPFNVTAQPEPTVEVEEFVYDSTKYCRPYRVYKNQIYIYPKHLKYDSQKCFNKARNITVCIEFKNSDDEGAKPMKCIYGKPGGPLFTSAAYTAVLHHSQNPDFSDEVKIELPTQLHEKHHILFSFYHVTCDINAKANAKKKEVLETSVGYAWLPLMKHDQIASQEYNIPIATSLPPNYLSFQDSASGKHGGSDIKWVDGGKPLFKVSTFVVSTVNTQDPYVNAFFRQCQKREKDMSQSPTSNFIRSCKNLLNVEKIHAIMSFLPIILNQLFKVLVQNEEDEISTTVTRVLTDIVAKCHEEQLDHSVQSYIKFVFKTKACKERTIHEELTKSVTGLLKSNDSTTVRHVLKHSWFFFAIILKSMAQHLIDTNKIQLSRAQRFPESYQNELDNLIMVLSDHVIWKYKDALEETRRANHSVARFLKRCFTFMDRGFVFKMVNNYISMFSSGELKTLCQYKFDFLQEVCQHEHFIPLCLPIRSANIPDPLTPSESTQELHASDMPEYSVTNEFCRKHFLIGILLREVGFALQEDQDIRHLALAVLKNLMAKHSFDDRYREPRKQAQIASLYMPLYGMLLDNMPRIYLKDLYPFTINTSNQGSRDDLSTNGGFHSQSAMKHANSVDTSFSKDVLNSIAAFSSIAISTVNHADSRASLASLDSNPSTNEKSSEKTDNCEKIPRPLSLIGSTLRFDKLDQAETRSLLMCFLQIMKTISEETLIAYWQRAPSPEVSDFFSILDVCLQNFRYLGKRNIIRKIAAAFKFVQSTQNNGTLKGSNPSCQTSGLLTQWMNTTSGHEGHKQHRSQTLPIIRGKNALSNPKLLQMLDNTMTSNSNEIDIVHHVDTEANVATEVCLTILDLLSLFTQIHHRQLQQSECQNSLMKRVFDTYMLFFQVNQSATALKHVFASLRLFVCKFPAAFFQGPADLCGSFCYEVLKCCNHRSRSTQTEASALLYFFMRKNFEFNKQKSIVRSHLQLIKAVSQLIADAGIGGSRFQHSLAITNNFANGDKQMKNSNFPAEVKDLTKRIRTVLMATAQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHARNGDLSEAAMCYIHIAALIAEYLKRKGYWKMEKICTPSLLLEDIHPCDSIPLLTTPSGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQDTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVNPKDLDPKYAYIQVTYVTPFFEEKEIEDRKTDFEMHHNIKRFVFETPFTLSGKKHGGVEEQCKRRTVLTTSHLFPYVKKRIQVISQSSTELNPIEVAIDEMSKKVSELNQLCTMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEETNAKKYPDNQVKLLKEIFRQFADACGQALDVNERLIKEDQLEYQEELRSHYKDMLGELSAIMNEQITYRDDLAKRGLERTFTRVTSKATAAPPTVSISPCVEI

Target ORF information:

RefSeq Version	XM_023532929.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023532929.1

ORF Insert Sequence:

ATGGGCTGCA CCGCCAGCAT TGTTCTGCTA CAAGCTTTTC GTTCTGTGGT CCAGAGGAGC 
TGTCCACACA TTTGCAGCCG ACGCCAGGAG GAACCATCCC ATGAAATTTT GCCTCTAGAA 
AGTGACGATG AAATGAGCAG ACCCAGAACC CTCATCATAA TGCAGGAAAA GCCCAGGCTC 
CTTGAACCCT TGGATTATGA AACTGTCATT GAAGAACTTG AAAAGACCTA TCAGAATGAC 
CCTCTTCAAG ATCTCCTGTT CTTCCCCAGT GACGACTTTT CAACGGCCAC AGTTCCTTGG 
GACATTCGAA CACTGTACTC TACGGTACCT GAAGACGCAG AGCACAAAGC AGAAAATTTA 
CTGGTCAAAG AGGCTTGTAA ATTTTATAGT TCCCAGTGGT ACGTGGTAAA CTACAAATAT 
GAACAATATT CTGGAGATAT TCGCCAGTTA CCCCGAGCAG AATACAAACC AGAGAAACTC 
CCTTCGCATT CCTTTGAGGT TGACCATGAA GATGCTGATA AGGATGAAGA TACCACCTCC 
CACTCATCTT CCAAGGGCGG TGGGGGAGCA GGGGGAACTG GAGTCTTCAA GTCAGGCTGG 
CTCTACAAGG GGAATTTCAA CAGCACCGTG AACAATACCA TCACTGTTCG GTCATTCAAG 
AAGCGCTACT TCCAGCTGAC TCAGCTGCCA GATAACTCCT ACATCATGAA TTTTTACAAA 
GATGAAAAAA TATCCAAAGA GCCCAAAGGC TGCATCTTTT TGGATTCCTG TACAGGTGTG 
GTGCAGAATA ACAGACTAAG AAAATATGCC TTTGAATTGA AAATGAATGA CCTGACCTAT 
TTTGTGCTGG CGGCGGAAAC AGAGTGCGAC ATGGAGGAAT GGATCCACAC CCTGAACCGC 
ATCCTGCAAA TCAGCCCTGA GGGGCCCCTG CAGGGTCGAC GGAGCGCGGA GCTCACGGAT 
CTGGGCCTGG ATTCACTAGA TAATTCTATC ACATGTGAAT GCGCACCAGA GGAAACAGAT 
TCTTCAGAGA ACAGCCTACA TCCAGACTTC GCAAAATACC TCACAGAAAC TGAAGAGACT 
GTCAAAGCAA CTCGAAACAT GGAGAGACTC AATCTGTTCT CGATGGACCC AGATATAGAT 
ACCTTGAAAC TTCAGAAAAA GGATCTTTTG GAATCCGAGT CTGTGATCAA ACCATTTGAA 
GAAAAAGCTG CCAAGAGAAT CATGATCATA TGTAAAGCTC TCACCTTCAG TCTTCAGGGA 
TGTGTTACAG AGAATGAAAA TGATCCCATA ACCAACATTG AGCCTTTTTT TGTGAGTGTG 
GCACTTTATG ACCTCAGAAA CAGCAGGAAG ATTTCTGCCG ACTTCCACGT GGATCTAAAC 
CACACTGCTG TCAGGCAGAT GCTCTCAGGG GCTCCCGTGG CTTTGGAAAA TGGAAACAGC 
GACACGGTCA CCCCAAGACA ATCAGAAGAA CCTCACGTCA AGGGGTTTCC AGAGGAATGG 
CTGAAATTTC CAAAGCAGGC TATATTTTCT GTAAGCAATC CACATTCGGA AATTGTCTTG 
GTGGCTAAAA TTGAAAAAGT ACTAATGGGA AACATTGCAA GTGGTGCTGA ACCTTACATT 
AAGAACCCAG ACTCCAACAA GTATGCACAA AAGATACTCA AATCCAACAG ACAGTTCTGC 
AGCAAGTTGG GGAAATACCG CATGCCGTTT GCTTGGGCGA TGAGATCAGT TTTTAAGGAC 
AACCAGGGAA ATGTGGACAG AGACTCAAGA TTTTCACCAT TGTATAGACA CGAAAGTAGC 
AAGATTTCAA CTGAGGACCT ACTTAAACTA GTGTCAGATT ATAGGAGGGC CGACAGAATA 
AGCAAAATGC AGACCATTCC AGGAAGCCTC GATATCACTG TTGAGAACAT TCCTCTGGAG 
CATCCAAACT GTGTAACATC GTCCTTTATC CCCGTCAAGC CTTTCAACGT GACGGCTCAA 
CCGGAACCCA CAGTGGAGGT GGAAGAATTT GTTTATGACT CAACAAAGTA CTGCCGTCCT 
TACAGAGTAT ACAAAAATCA AATTTACATT TACCCCAAGC ACCTCAAGTA TGATAGCCAG 
AAATGTTTCA ACAAGGCACG AAATATAACC GTGTGCATTG AATTCAAAAA TTCTGACGAC 
GAAGGTGCCA AGCCCATGAA GTGTATTTAT GGGAAGCCTG GAGGGCCCCT CTTCACCTCA 
GCCGCCTACA CAGCAGTTCT GCACCATTCC CAGAATCCGG ATTTCTCAGA CGAGGTGAAA 
ATTGAGCTAC CAACACAACT CCACGAGAAA CACCATATTC TGTTTTCTTT TTATCATGTC 
ACTTGCGACA TAAATGCAAA AGCTAATGCC AAAAAGAAGG AAGTTCTGGA AACATCAGTT 
GGATATGCTT GGCTTCCTCT AATGAAACAT GATCAGATAG CTTCTCAAGA GTACAATATC 
CCAATAGCTA CAAGCCTGCC TCCTAATTAT TTAAGCTTTC AAGATTCTGC AAGTGGAAAG 
CATGGTGGGA GTGACATTAA ATGGGTTGAT GGTGGCAAAC CACTTTTCAA AGTATCAACA 
TTCGTTGTAT CAACAGTGAA TACTCAGGAT CCGTATGTGA ACGCGTTTTT CCGACAGTGC 
CAAAAAAGAG AGAAAGATAT GTCTCAGTCA CCTACCTCGA ATTTCATCCG CTCTTGTAAG 
AACTTATTGA ATGTGGAAAA AATTCACGCA ATCATGAGTT TTCTGCCTAT AATCTTGAAT 
CAGCTCTTCA AGGTTTTGGT ACAGAATGAG GAAGATGAAA TAAGTACAAC TGTGACCAGG 
GTTCTGACTG ACATTGTGGC CAAGTGCCAC GAGGAGCAGC TGGACCACTC CGTCCAGTCA 
TACATTAAGT TTGTGTTCAA GACCAAGGCT TGCAAGGAGA GAACGATACA CGAGGAACTG 
ACTAAGAGTG TGACTGGTCT TTTGAAATCA AATGACTCCA CAACAGTCAG GCACGTTCTG 
AAGCATTCCT GGTTCTTCTT CGCAATTATT CTAAAATCGA TGGCACAACA CTTGATTGAT 
ACAAATAAAA TTCAGCTTTC TCGGGCTCAA AGATTTCCTG AATCTTACCA GAATGAATTG 
GACAATCTTA TAATGGTCCT ATCTGATCAC GTGATCTGGA AATACAAAGA TGCCCTTGAA 
GAAACAAGAA GGGCCAACCA CAGCGTTGCC AGATTTCTCA AGCGCTGCTT CACCTTCATG 
GATCGGGGCT TTGTGTTTAA GATGGTTAAC AATTACATCA GCATGTTCTC CTCTGGTGAG 
CTCAAGACCT TATGCCAGTA TAAATTTGAT TTTCTTCAAG AAGTATGTCA GCATGAACAT 
TTTATTCCTT TGTGTCTCCC CATAAGATCA GCAAACATTC CAGATCCTCT GACACCTTCG 
GAATCAACTC AAGAGCTACA TGCATCAGAT ATGCCTGAAT ATTCGGTCAC GAATGAATTT 
TGCCGCAAGC ACTTCTTAAT CGGGATTCTG CTCCGAGAAG TTGGCTTTGC TCTGCAGGAA 
GACCAAGATA TCAGGCACTT AGCTTTAGCT GTCCTGAAAA ATCTAATGGC TAAGCATTCG 
TTTGATGATC GATACAGAGA ACCAAGAAAG CAGGCCCAGA TAGCAAGCTT ATACATGCCC 
CTCTATGGCA TGCTTCTGGA CAACATGCCC AGGATTTACC TGAAGGATCT ATATCCTTTC 
ACTATCAATA CATCAAACCA GGGGTCTAGA GATGACCTCA GCACCAACGG AGGATTTCAC 
AGCCAGTCAG CTATGAAACA TGCAAACTCT GTGGACACAT CATTTTCCAA AGATGTTTTA 
AATTCCATAG CAGCATTTTC ATCAATAGCT ATTTCTACAG TAAACCATGC TGATTCCAGA 
GCATCCTTAG CAAGTCTGGA CTCCAATCCA AGTACCAATG AGAAGAGCAG TGAGAAAACT 
GACAACTGTG AAAAGATCCC AAGACCCTTG TCTTTGATAG GCTCAACGCT TCGATTTGAC 
AAGTTAGATC AAGCAGAAAC CAGGAGCCTT CTTATGTGCT TTCTTCAAAT TATGAAGACA 
ATTTCAGAGG AGACTCTGAT TGCCTACTGG CAAAGAGCAC CTAGTCCGGA AGTGTCTGAC 
TTCTTCAGCA TCTTGGATGT TTGTCTTCAA AATTTCAGAT ACCTAGGAAA ACGCAATATA 
ATAAGGAAAA TTGCTGCTGC ATTTAAATTT GTGCAGTCCA CCCAGAACAA CGGAACTCTC 
AAAGGATCCA ATCCTTCTTG CCAAACATCG GGTCTCTTGA CACAATGGAT GAACACCACT 
TCTGGTCACG AAGGACATAA GCAGCACAGA TCACAAACTT TACCTATAAT TCGAGGCAAA 
AATGCACTCT CTAACCCCAA ACTCTTACAG ATGCTAGACA ATACCATGAC CAGCAACTCC 
AATGAAATAG ACATCGTGCA CCACGTGGAC ACAGAGGCCA ATGTAGCTAC AGAGGTTTGC 
CTTACTATTC TAGACCTGCT GTCCCTCTTC ACTCAGATTC ACCATAGGCA ACTCCAACAA 
TCTGAATGTC AAAATTCATT GATGAAAAGG GTCTTTGATA CCTACATGCT CTTCTTCCAA 
GTCAACCAGT CAGCCACAGC ACTGAAGCAT GTGTTTGCTT CCCTGAGACT GTTTGTATGC 
AAGTTTCCCG CAGCGTTCTT TCAAGGGCCT GCTGACCTGT GTGGATCATT CTGTTACGAA 
GTCCTAAAAT GCTGTAACCA CAGGTCACGG TCAACCCAGA CAGAAGCATC GGCACTTCTA 
TACTTTTTCA TGAGGAAGAA TTTTGAATTT AATAAGCAGA AGTCAATTGT CCGATCCCAC 
CTACAGCTCA TCAAGGCTGT AAGCCAGTTA ATAGCCGATG CTGGGATTGG AGGCTCTCGG 
TTTCAACATT CCCTTGCAAT TACCAATAAT TTTGCTAATG GAGACAAGCA AATGAAAAAC 
AGCAATTTCC CAGCAGAGGT GAAAGATCTG ACTAAACGTA TAAGGACTGT TCTGATGGCC 
ACGGCTCAGA TGAAGGAGCA TGAGAAGGAC CCCGAGATGC TGGTGGACCT CCAGTATAGC 
CTGGCAAACT CCTATGCGAG CACCCCTGAG CTACGGAGGA CTTGGCTGGA AAGCATGGCC 
AAGATTCACG CAAGAAATGG AGACTTGTCC GAGGCTGCCA TGTGCTACAT CCACATAGCA 
GCCCTCATCG CAGAATACCT GAAAAGAAAG GGTTACTGGA AAATGGAAAA GATTTGCACG 
CCATCCCTGC TCCTGGAGGA TATCCATCCC TGTGATAGCA TCCCATTACT AACAACTCCC 
AGTGGAGGAA GCATGTTCTC CATGGGCTGG CCAGCTTTTC TGAGCATTAC ACCAAATATT 
AAAGAAGAAG GAGCAATGAA AGAAGATTCG GGAATGCAAG ACACACCATA CAATGAGAAT 
ATCCTGGTGG AACAGCTGTA TATGTGTGTG GAGTTTCTTT GGAAGTCTGA GAGATATGAA 
CTCATCGCTG ATGTCAACAA ACCCATCATC GCTGTCTTTG AGAAGCAGAG AGACTTCAAA 
AAATTGTCAG ATCTCTACTA TGACATTCAC CGGTCATACC TGAAAGTCGC AGAGGTGGTG 
AATTCAGAGA AGCGACTGTT TGGTCGGTAC TATCGCGTGG CGTTTTACGG GCAGGGGTTT 
TTTGAAGAAG AAGAAGGTAA AGAGTATATT TATAAAGAGC CTAAGCTGAC AGGTCTCTCC 
GAGATTTCCC AAAGACTCCT CAAGCTGTAT GCAGATAAAT TCGGAGCAGA CAATGTGAAG 
ATAATCCAGG ATTCCAACAA GGTAAACCCG AAGGACTTGG ACCCCAAATA TGCCTACATC 
CAGGTGACCT ACGTCACTCC ATTCTTTGAG GAAAAGGAAA TAGAGGACCG GAAGACGGAT 
TTCGAAATGC ACCACAACAT CAAGCGCTTT GTCTTTGAGA CGCCCTTCAC GCTGTCGGGC 
AAGAAGCATG GTGGCGTGGA GGAGCAGTGC AAGCGACGGA CGGTGCTGAC CACAAGTCAC 
CTGTTCCCCT ACGTGAAGAA GAGAATACAG GTCATAAGCC AGTCGAGCAC AGAACTGAAT 
CCTATTGAGG TGGCGATTGA TGAGATGTCC AAGAAAGTTT CTGAGCTTAA TCAGCTCTGC 
ACGATGGAGG AAGTGGACAT GATCAGACTG CAGCTCAAAC TCCAAGGAAG TGTCAGCGTG 
AAGGTTAATG CTGGACCAAT GGCCTATGCG CGAGCTTTTC TTGAAGAAAC CAACGCAAAG 
AAATACCCTG ACAACCAAGT AAAGCTTTTG AAGGAAATCT TCAGGCAATT TGCAGATGCG 
TGTGGGCAGG CCCTGGACGT GAATGAGCGC CTCATCAAGG AGGACCAGCT GGAGTACCAG 
GAAGAACTGA GGTCTCATTA TAAGGACATG CTCGGCGAAC TCTCTGCAAT TATGAATGAG 
CAGATTACGT ACAGGGACGA CCTGGCAAAG CGCGGATTGG AACGAACCTT CACTCGAGTA 
ACTAGCAAGG CAACTGCAGC TCCGCCCACG GTGTCCATCT CACCTTGCGT GGAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_023532930.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023388698.1 Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X2, mRNA.

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CloneID	OPt70924
Clone ID Related Accession (Same CDS sequence)	XM_023532929.1 , XM_023532930.1
Accession Version	XM_023532930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	dedicator of cytokinesis protein 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888874.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCA CCGCCAGCAT TGTTCTGCTA CAAGCTTTTC GTTCTGTGGT CCAGAGGAGC 
TGTCCACACA TTTGCAGCCG ACGCCAGGAG GAACCATCCC ATGAAATTTT GCCTCTAGAA 
AGTGACGATG AAATGAGCAG ACCCAGAACC CTCATCATAA TGCAGGAAAA GCCCAGGCTC 
CTTGAACCCT TGGATTATGA AACTGTCATT GAAGAACTTG AAAAGACCTA TCAGAATGAC 
CCTCTTCAAG ATCTCCTGTT CTTCCCCAGT GACGACTTTT CAACGGCCAC AGTTCCTTGG 
GACATTCGAA CACTGTACTC TACGGTACCT GAAGACGCAG AGCACAAAGC AGAAAATTTA 
CTGGTCAAAG AGGCTTGTAA ATTTTATAGT TCCCAGTGGT ACGTGGTAAA CTACAAATAT 
GAACAATATT CTGGAGATAT TCGCCAGTTA CCCCGAGCAG AATACAAACC AGAGAAACTC 
CCTTCGCATT CCTTTGAGGT TGACCATGAA GATGCTGATA AGGATGAAGA TACCACCTCC 
CACTCATCTT CCAAGGGCGG TGGGGGAGCA GGGGGAACTG GAGTCTTCAA GTCAGGCTGG 
CTCTACAAGG GGAATTTCAA CAGCACCGTG AACAATACCA TCACTGTTCG GTCATTCAAG 
AAGCGCTACT TCCAGCTGAC TCAGCTGCCA GATAACTCCT ACATCATGAA TTTTTACAAA 
GATGAAAAAA TATCCAAAGA GCCCAAAGGC TGCATCTTTT TGGATTCCTG TACAGGTGTG 
GTGCAGAATA ACAGACTAAG AAAATATGCC TTTGAATTGA AAATGAATGA CCTGACCTAT 
TTTGTGCTGG CGGCGGAAAC AGAGTGCGAC ATGGAGGAAT GGATCCACAC CCTGAACCGC 
ATCCTGCAAA TCAGCCCTGA GGGGCCCCTG CAGGGTCGAC GGAGCGCGGA GCTCACGGAT 
CTGGGCCTGG ATTCACTAGA TAATTCTATC ACATGTGAAT GCGCACCAGA GGAAACAGAT 
TCTTCAGAGA ACAGCCTACA TCCAGACTTC GCAAAATACC TCACAGAAAC TGAAGAGACT 
GTCAAAGCAA CTCGAAACAT GGAGAGACTC AATCTGTTCT CGATGGACCC AGATATAGAT 
ACCTTGAAAC TTCAGAAAAA GGATCTTTTG GAATCCGAGT CTGTGATCAA ACCATTTGAA 
GAAAAAGCTG CCAAGAGAAT CATGATCATA TGTAAAGCTC TCACCTTCAG TCTTCAGGGA 
TGTGTTACAG AGAATGAAAA TGATCCCATA ACCAACATTG AGCCTTTTTT TGTGAGTGTG 
GCACTTTATG ACCTCAGAAA CAGCAGGAAG ATTTCTGCCG ACTTCCACGT GGATCTAAAC 
CACACTGCTG TCAGGCAGAT GCTCTCAGGG GCTCCCGTGG CTTTGGAAAA TGGAAACAGC 
GACACGGTCA CCCCAAGACA ATCAGAAGAA CCTCACGTCA AGGGGTTTCC AGAGGAATGG 
CTGAAATTTC CAAAGCAGGC TATATTTTCT GTAAGCAATC CACATTCGGA AATTGTCTTG 
GTGGCTAAAA TTGAAAAAGT ACTAATGGGA AACATTGCAA GTGGTGCTGA ACCTTACATT 
AAGAACCCAG ACTCCAACAA GTATGCACAA AAGATACTCA AATCCAACAG ACAGTTCTGC 
AGCAAGTTGG GGAAATACCG CATGCCGTTT GCTTGGGCGA TGAGATCAGT TTTTAAGGAC 
AACCAGGGAA ATGTGGACAG AGACTCAAGA TTTTCACCAT TGTATAGACA CGAAAGTAGC 
AAGATTTCAA CTGAGGACCT ACTTAAACTA GTGTCAGATT ATAGGAGGGC CGACAGAATA 
AGCAAAATGC AGACCATTCC AGGAAGCCTC GATATCACTG TTGAGAACAT TCCTCTGGAG 
CATCCAAACT GTGTAACATC GTCCTTTATC CCCGTCAAGC CTTTCAACGT GACGGCTCAA 
CCGGAACCCA CAGTGGAGGT GGAAGAATTT GTTTATGACT CAACAAAGTA CTGCCGTCCT 
TACAGAGTAT ACAAAAATCA AATTTACATT TACCCCAAGC ACCTCAAGTA TGATAGCCAG 
AAATGTTTCA ACAAGGCACG AAATATAACC GTGTGCATTG AATTCAAAAA TTCTGACGAC 
GAAGGTGCCA AGCCCATGAA GTGTATTTAT GGGAAGCCTG GAGGGCCCCT CTTCACCTCA 
GCCGCCTACA CAGCAGTTCT GCACCATTCC CAGAATCCGG ATTTCTCAGA CGAGGTGAAA 
ATTGAGCTAC CAACACAACT CCACGAGAAA CACCATATTC TGTTTTCTTT TTATCATGTC 
ACTTGCGACA TAAATGCAAA AGCTAATGCC AAAAAGAAGG AAGTTCTGGA AACATCAGTT 
GGATATGCTT GGCTTCCTCT AATGAAACAT GATCAGATAG CTTCTCAAGA GTACAATATC 
CCAATAGCTA CAAGCCTGCC TCCTAATTAT TTAAGCTTTC AAGATTCTGC AAGTGGAAAG 
CATGGTGGGA GTGACATTAA ATGGGTTGAT GGTGGCAAAC CACTTTTCAA AGTATCAACA 
TTCGTTGTAT CAACAGTGAA TACTCAGGAT CCGTATGTGA ACGCGTTTTT CCGACAGTGC 
CAAAAAAGAG AGAAAGATAT GTCTCAGTCA CCTACCTCGA ATTTCATCCG CTCTTGTAAG 
AACTTATTGA ATGTGGAAAA AATTCACGCA ATCATGAGTT TTCTGCCTAT AATCTTGAAT 
CAGCTCTTCA AGGTTTTGGT ACAGAATGAG GAAGATGAAA TAAGTACAAC TGTGACCAGG 
GTTCTGACTG ACATTGTGGC CAAGTGCCAC GAGGAGCAGC TGGACCACTC CGTCCAGTCA 
TACATTAAGT TTGTGTTCAA GACCAAGGCT TGCAAGGAGA GAACGATACA CGAGGAACTG 
ACTAAGAGTG TGACTGGTCT TTTGAAATCA AATGACTCCA CAACAGTCAG GCACGTTCTG 
AAGCATTCCT GGTTCTTCTT CGCAATTATT CTAAAATCGA TGGCACAACA CTTGATTGAT 
ACAAATAAAA TTCAGCTTTC TCGGGCTCAA AGATTTCCTG AATCTTACCA GAATGAATTG 
GACAATCTTA TAATGGTCCT ATCTGATCAC GTGATCTGGA AATACAAAGA TGCCCTTGAA 
GAAACAAGAA GGGCCAACCA CAGCGTTGCC AGATTTCTCA AGCGCTGCTT CACCTTCATG 
GATCGGGGCT TTGTGTTTAA GATGGTTAAC AATTACATCA GCATGTTCTC CTCTGGTGAG 
CTCAAGACCT TATGCCAGTA TAAATTTGAT TTTCTTCAAG AAGTATGTCA GCATGAACAT 
TTTATTCCTT TGTGTCTCCC CATAAGATCA GCAAACATTC CAGATCCTCT GACACCTTCG 
GAATCAACTC AAGAGCTACA TGCATCAGAT ATGCCTGAAT ATTCGGTCAC GAATGAATTT 
TGCCGCAAGC ACTTCTTAAT CGGGATTCTG CTCCGAGAAG TTGGCTTTGC TCTGCAGGAA 
GACCAAGATA TCAGGCACTT AGCTTTAGCT GTCCTGAAAA ATCTAATGGC TAAGCATTCG 
TTTGATGATC GATACAGAGA ACCAAGAAAG CAGGCCCAGA TAGCAAGCTT ATACATGCCC 
CTCTATGGCA TGCTTCTGGA CAACATGCCC AGGATTTACC TGAAGGATCT ATATCCTTTC 
ACTATCAATA CATCAAACCA GGGGTCTAGA GATGACCTCA GCACCAACGG AGGATTTCAC 
AGCCAGTCAG CTATGAAACA TGCAAACTCT GTGGACACAT CATTTTCCAA AGATGTTTTA 
AATTCCATAG CAGCATTTTC ATCAATAGCT ATTTCTACAG TAAACCATGC TGATTCCAGA 
GCATCCTTAG CAAGTCTGGA CTCCAATCCA AGTACCAATG AGAAGAGCAG TGAGAAAACT 
GACAACTGTG AAAAGATCCC AAGACCCTTG TCTTTGATAG GCTCAACGCT TCGATTTGAC 
AAGTTAGATC AAGCAGAAAC CAGGAGCCTT CTTATGTGCT TTCTTCAAAT TATGAAGACA 
ATTTCAGAGG AGACTCTGAT TGCCTACTGG CAAAGAGCAC CTAGTCCGGA AGTGTCTGAC 
TTCTTCAGCA TCTTGGATGT TTGTCTTCAA AATTTCAGAT ACCTAGGAAA ACGCAATATA 
ATAAGGAAAA TTGCTGCTGC ATTTAAATTT GTGCAGTCCA CCCAGAACAA CGGAACTCTC 
AAAGGATCCA ATCCTTCTTG CCAAACATCG GGTCTCTTGA CACAATGGAT GAACACCACT 
TCTGGTCACG AAGGACATAA GCAGCACAGA TCACAAACTT TACCTATAAT TCGAGGCAAA 
AATGCACTCT CTAACCCCAA ACTCTTACAG ATGCTAGACA ATACCATGAC CAGCAACTCC 
AATGAAATAG ACATCGTGCA CCACGTGGAC ACAGAGGCCA ATGTAGCTAC AGAGGTTTGC 
CTTACTATTC TAGACCTGCT GTCCCTCTTC ACTCAGATTC ACCATAGGCA ACTCCAACAA 
TCTGAATGTC AAAATTCATT GATGAAAAGG GTCTTTGATA CCTACATGCT CTTCTTCCAA 
GTCAACCAGT CAGCCACAGC ACTGAAGCAT GTGTTTGCTT CCCTGAGACT GTTTGTATGC 
AAGTTTCCCG CAGCGTTCTT TCAAGGGCCT GCTGACCTGT GTGGATCATT CTGTTACGAA 
GTCCTAAAAT GCTGTAACCA CAGGTCACGG TCAACCCAGA CAGAAGCATC GGCACTTCTA 
TACTTTTTCA TGAGGAAGAA TTTTGAATTT AATAAGCAGA AGTCAATTGT CCGATCCCAC 
CTACAGCTCA TCAAGGCTGT AAGCCAGTTA ATAGCCGATG CTGGGATTGG AGGCTCTCGG 
TTTCAACATT CCCTTGCAAT TACCAATAAT TTTGCTAATG GAGACAAGCA AATGAAAAAC 
AGCAATTTCC CAGCAGAGGT GAAAGATCTG ACTAAACGTA TAAGGACTGT TCTGATGGCC 
ACGGCTCAGA TGAAGGAGCA TGAGAAGGAC CCCGAGATGC TGGTGGACCT CCAGTATAGC 
CTGGCAAACT CCTATGCGAG CACCCCTGAG CTACGGAGGA CTTGGCTGGA AAGCATGGCC 
AAGATTCACG CAAGAAATGG AGACTTGTCC GAGGCTGCCA TGTGCTACAT CCACATAGCA 
GCCCTCATCG CAGAATACCT GAAAAGAAAG GGTTACTGGA AAATGGAAAA GATTTGCACG 
CCATCCCTGC TCCTGGAGGA TATCCATCCC TGTGATAGCA TCCCATTACT AACAACTCCC 
AGTGGAGGAA GCATGTTCTC CATGGGCTGG CCAGCTTTTC TGAGCATTAC ACCAAATATT 
AAAGAAGAAG GAGCAATGAA AGAAGATTCG GGAATGCAAG ACACACCATA CAATGAGAAT 
ATCCTGGTGG AACAGCTGTA TATGTGTGTG GAGTTTCTTT GGAAGTCTGA GAGATATGAA 
CTCATCGCTG ATGTCAACAA ACCCATCATC GCTGTCTTTG AGAAGCAGAG AGACTTCAAA 
AAATTGTCAG ATCTCTACTA TGACATTCAC CGGTCATACC TGAAAGTCGC AGAGGTGGTG 
AATTCAGAGA AGCGACTGTT TGGTCGGTAC TATCGCGTGG CGTTTTACGG GCAGGGGTTT 
TTTGAAGAAG AAGAAGGTAA AGAGTATATT TATAAAGAGC CTAAGCTGAC AGGTCTCTCC 
GAGATTTCCC AAAGACTCCT CAAGCTGTAT GCAGATAAAT TCGGAGCAGA CAATGTGAAG 
ATAATCCAGG ATTCCAACAA GGTAAACCCG AAGGACTTGG ACCCCAAATA TGCCTACATC 
CAGGTGACCT ACGTCACTCC ATTCTTTGAG GAAAAGGAAA TAGAGGACCG GAAGACGGAT 
TTCGAAATGC ACCACAACAT CAAGCGCTTT GTCTTTGAGA CGCCCTTCAC GCTGTCGGGC 
AAGAAGCATG GTGGCGTGGA GGAGCAGTGC AAGCGACGGA CGGTGCTGAC CACAAGTCAC 
CTGTTCCCCT ACGTGAAGAA GAGAATACAG GTCATAAGCC AGTCGAGCAC AGAACTGAAT 
CCTATTGAGG TGGCGATTGA TGAGATGTCC AAGAAAGTTT CTGAGCTTAA TCAGCTCTGC 
ACGATGGAGG AAGTGGACAT GATCAGACTG CAGCTCAAAC TCCAAGGAAG TGTCAGCGTG 
AAGGTTAATG CTGGACCAAT GGCCTATGCG CGAGCTTTTC TTGAAGAAAC CAACGCAAAG 
AAATACCCTG ACAACCAAGT AAAGCTTTTG AAGGAAATCT TCAGGCAATT TGCAGATGCG 
TGTGGGCAGG CCCTGGACGT GAATGAGCGC CTCATCAAGG AGGACCAGCT GGAGTACCAG 
GAAGAACTGA GGTCTCATTA TAAGGACATG CTCGGCGAAC TCTCTGCAAT TATGAATGAG 
CAGATTACGT ACAGGGACGA CCTGGCAAAG CGCGGATTGG AACGAACCTT CACTCGAGTA 
ACTAGCAAGG CAACTGCAGC TCCGCCCACG GTGTCCATCT CACCTTGCGT GGAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023388698.1
CDS	16..6615
Translation	MGCTASIVLLQAFRSVVQRSCPHICSRRQEEPSHEILPLESDDEMSRPRTLIIMQEKPRLLEPLDYETVIEELEKTYQNDPLQDLLFFPSDDFSTATVPWDIRTLYSTVPEDAEHKAENLLVKEACKFYSSQWYVVNYKYEQYSGDIRQLPRAEYKPEKLPSHSFEVDHEDADKDEDTTSHSSSKGGGGAGGTGVFKSGWLYKGNFNSTVNNTITVRSFKKRYFQLTQLPDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKYAFELKMNDLTYFVLAAETECDMEEWIHTLNRILQISPEGPLQGRRSAELTDLGLDSLDNSITCECAPEETDSSENSLHPDFAKYLTETEETVKATRNMERLNLFSMDPDIDTLKLQKKDLLESESVIKPFEEKAAKRIMIICKALTFSLQGCVTENENDPITNIEPFFVSVALYDLRNSRKISADFHVDLNHTAVRQMLSGAPVALENGNSDTVTPRQSEEPHVKGFPEEWLKFPKQAIFSVSNPHSEIVLVAKIEKVLMGNIASGAEPYIKNPDSNKYAQKILKSNRQFCSKLGKYRMPFAWAMRSVFKDNQGNVDRDSRFSPLYRHESSKISTEDLLKLVSDYRRADRISKMQTIPGSLDITVENIPLEHPNCVTSSFIPVKPFNVTAQPEPTVEVEEFVYDSTKYCRPYRVYKNQIYIYPKHLKYDSQKCFNKARNITVCIEFKNSDDEGAKPMKCIYGKPGGPLFTSAAYTAVLHHSQNPDFSDEVKIELPTQLHEKHHILFSFYHVTCDINAKANAKKKEVLETSVGYAWLPLMKHDQIASQEYNIPIATSLPPNYLSFQDSASGKHGGSDIKWVDGGKPLFKVSTFVVSTVNTQDPYVNAFFRQCQKREKDMSQSPTSNFIRSCKNLLNVEKIHAIMSFLPIILNQLFKVLVQNEEDEISTTVTRVLTDIVAKCHEEQLDHSVQSYIKFVFKTKACKERTIHEELTKSVTGLLKSNDSTTVRHVLKHSWFFFAIILKSMAQHLIDTNKIQLSRAQRFPESYQNELDNLIMVLSDHVIWKYKDALEETRRANHSVARFLKRCFTFMDRGFVFKMVNNYISMFSSGELKTLCQYKFDFLQEVCQHEHFIPLCLPIRSANIPDPLTPSESTQELHASDMPEYSVTNEFCRKHFLIGILLREVGFALQEDQDIRHLALAVLKNLMAKHSFDDRYREPRKQAQIASLYMPLYGMLLDNMPRIYLKDLYPFTINTSNQGSRDDLSTNGGFHSQSAMKHANSVDTSFSKDVLNSIAAFSSIAISTVNHADSRASLASLDSNPSTNEKSSEKTDNCEKIPRPLSLIGSTLRFDKLDQAETRSLLMCFLQIMKTISEETLIAYWQRAPSPEVSDFFSILDVCLQNFRYLGKRNIIRKIAAAFKFVQSTQNNGTLKGSNPSCQTSGLLTQWMNTTSGHEGHKQHRSQTLPIIRGKNALSNPKLLQMLDNTMTSNSNEIDIVHHVDTEANVATEVCLTILDLLSLFTQIHHRQLQQSECQNSLMKRVFDTYMLFFQVNQSATALKHVFASLRLFVCKFPAAFFQGPADLCGSFCYEVLKCCNHRSRSTQTEASALLYFFMRKNFEFNKQKSIVRSHLQLIKAVSQLIADAGIGGSRFQHSLAITNNFANGDKQMKNSNFPAEVKDLTKRIRTVLMATAQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHARNGDLSEAAMCYIHIAALIAEYLKRKGYWKMEKICTPSLLLEDIHPCDSIPLLTTPSGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQDTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVNPKDLDPKYAYIQVTYVTPFFEEKEIEDRKTDFEMHHNIKRFVFETPFTLSGKKHGGVEEQCKRRTVLTTSHLFPYVKKRIQVISQSSTELNPIEVAIDEMSKKVSELNQLCTMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEETNAKKYPDNQVKLLKEIFRQFADACGQALDVNERLIKEDQLEYQEELRSHYKDMLGELSAIMNEQITYRDDLAKRGLERTFTRVTSKATAAPPTVSISPCVEI

Target ORF information:

RefSeq Version	XM_023532930.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023532930.1

ORF Insert Sequence:

ATGGGCTGCA CCGCCAGCAT TGTTCTGCTA CAAGCTTTTC GTTCTGTGGT CCAGAGGAGC 
TGTCCACACA TTTGCAGCCG ACGCCAGGAG GAACCATCCC ATGAAATTTT GCCTCTAGAA 
AGTGACGATG AAATGAGCAG ACCCAGAACC CTCATCATAA TGCAGGAAAA GCCCAGGCTC 
CTTGAACCCT TGGATTATGA AACTGTCATT GAAGAACTTG AAAAGACCTA TCAGAATGAC 
CCTCTTCAAG ATCTCCTGTT CTTCCCCAGT GACGACTTTT CAACGGCCAC AGTTCCTTGG 
GACATTCGAA CACTGTACTC TACGGTACCT GAAGACGCAG AGCACAAAGC AGAAAATTTA 
CTGGTCAAAG AGGCTTGTAA ATTTTATAGT TCCCAGTGGT ACGTGGTAAA CTACAAATAT 
GAACAATATT CTGGAGATAT TCGCCAGTTA CCCCGAGCAG AATACAAACC AGAGAAACTC 
CCTTCGCATT CCTTTGAGGT TGACCATGAA GATGCTGATA AGGATGAAGA TACCACCTCC 
CACTCATCTT CCAAGGGCGG TGGGGGAGCA GGGGGAACTG GAGTCTTCAA GTCAGGCTGG 
CTCTACAAGG GGAATTTCAA CAGCACCGTG AACAATACCA TCACTGTTCG GTCATTCAAG 
AAGCGCTACT TCCAGCTGAC TCAGCTGCCA GATAACTCCT ACATCATGAA TTTTTACAAA 
GATGAAAAAA TATCCAAAGA GCCCAAAGGC TGCATCTTTT TGGATTCCTG TACAGGTGTG 
GTGCAGAATA ACAGACTAAG AAAATATGCC TTTGAATTGA AAATGAATGA CCTGACCTAT 
TTTGTGCTGG CGGCGGAAAC AGAGTGCGAC ATGGAGGAAT GGATCCACAC CCTGAACCGC 
ATCCTGCAAA TCAGCCCTGA GGGGCCCCTG CAGGGTCGAC GGAGCGCGGA GCTCACGGAT 
CTGGGCCTGG ATTCACTAGA TAATTCTATC ACATGTGAAT GCGCACCAGA GGAAACAGAT 
TCTTCAGAGA ACAGCCTACA TCCAGACTTC GCAAAATACC TCACAGAAAC TGAAGAGACT 
GTCAAAGCAA CTCGAAACAT GGAGAGACTC AATCTGTTCT CGATGGACCC AGATATAGAT 
ACCTTGAAAC TTCAGAAAAA GGATCTTTTG GAATCCGAGT CTGTGATCAA ACCATTTGAA 
GAAAAAGCTG CCAAGAGAAT CATGATCATA TGTAAAGCTC TCACCTTCAG TCTTCAGGGA 
TGTGTTACAG AGAATGAAAA TGATCCCATA ACCAACATTG AGCCTTTTTT TGTGAGTGTG 
GCACTTTATG ACCTCAGAAA CAGCAGGAAG ATTTCTGCCG ACTTCCACGT GGATCTAAAC 
CACACTGCTG TCAGGCAGAT GCTCTCAGGG GCTCCCGTGG CTTTGGAAAA TGGAAACAGC 
GACACGGTCA CCCCAAGACA ATCAGAAGAA CCTCACGTCA AGGGGTTTCC AGAGGAATGG 
CTGAAATTTC CAAAGCAGGC TATATTTTCT GTAAGCAATC CACATTCGGA AATTGTCTTG 
GTGGCTAAAA TTGAAAAAGT ACTAATGGGA AACATTGCAA GTGGTGCTGA ACCTTACATT 
AAGAACCCAG ACTCCAACAA GTATGCACAA AAGATACTCA AATCCAACAG ACAGTTCTGC 
AGCAAGTTGG GGAAATACCG CATGCCGTTT GCTTGGGCGA TGAGATCAGT TTTTAAGGAC 
AACCAGGGAA ATGTGGACAG AGACTCAAGA TTTTCACCAT TGTATAGACA CGAAAGTAGC 
AAGATTTCAA CTGAGGACCT ACTTAAACTA GTGTCAGATT ATAGGAGGGC CGACAGAATA 
AGCAAAATGC AGACCATTCC AGGAAGCCTC GATATCACTG TTGAGAACAT TCCTCTGGAG 
CATCCAAACT GTGTAACATC GTCCTTTATC CCCGTCAAGC CTTTCAACGT GACGGCTCAA 
CCGGAACCCA CAGTGGAGGT GGAAGAATTT GTTTATGACT CAACAAAGTA CTGCCGTCCT 
TACAGAGTAT ACAAAAATCA AATTTACATT TACCCCAAGC ACCTCAAGTA TGATAGCCAG 
AAATGTTTCA ACAAGGCACG AAATATAACC GTGTGCATTG AATTCAAAAA TTCTGACGAC 
GAAGGTGCCA AGCCCATGAA GTGTATTTAT GGGAAGCCTG GAGGGCCCCT CTTCACCTCA 
GCCGCCTACA CAGCAGTTCT GCACCATTCC CAGAATCCGG ATTTCTCAGA CGAGGTGAAA 
ATTGAGCTAC CAACACAACT CCACGAGAAA CACCATATTC TGTTTTCTTT TTATCATGTC 
ACTTGCGACA TAAATGCAAA AGCTAATGCC AAAAAGAAGG AAGTTCTGGA AACATCAGTT 
GGATATGCTT GGCTTCCTCT AATGAAACAT GATCAGATAG CTTCTCAAGA GTACAATATC 
CCAATAGCTA CAAGCCTGCC TCCTAATTAT TTAAGCTTTC AAGATTCTGC AAGTGGAAAG 
CATGGTGGGA GTGACATTAA ATGGGTTGAT GGTGGCAAAC CACTTTTCAA AGTATCAACA 
TTCGTTGTAT CAACAGTGAA TACTCAGGAT CCGTATGTGA ACGCGTTTTT CCGACAGTGC 
CAAAAAAGAG AGAAAGATAT GTCTCAGTCA CCTACCTCGA ATTTCATCCG CTCTTGTAAG 
AACTTATTGA ATGTGGAAAA AATTCACGCA ATCATGAGTT TTCTGCCTAT AATCTTGAAT 
CAGCTCTTCA AGGTTTTGGT ACAGAATGAG GAAGATGAAA TAAGTACAAC TGTGACCAGG 
GTTCTGACTG ACATTGTGGC CAAGTGCCAC GAGGAGCAGC TGGACCACTC CGTCCAGTCA 
TACATTAAGT TTGTGTTCAA GACCAAGGCT TGCAAGGAGA GAACGATACA CGAGGAACTG 
ACTAAGAGTG TGACTGGTCT TTTGAAATCA AATGACTCCA CAACAGTCAG GCACGTTCTG 
AAGCATTCCT GGTTCTTCTT CGCAATTATT CTAAAATCGA TGGCACAACA CTTGATTGAT 
ACAAATAAAA TTCAGCTTTC TCGGGCTCAA AGATTTCCTG AATCTTACCA GAATGAATTG 
GACAATCTTA TAATGGTCCT ATCTGATCAC GTGATCTGGA AATACAAAGA TGCCCTTGAA 
GAAACAAGAA GGGCCAACCA CAGCGTTGCC AGATTTCTCA AGCGCTGCTT CACCTTCATG 
GATCGGGGCT TTGTGTTTAA GATGGTTAAC AATTACATCA GCATGTTCTC CTCTGGTGAG 
CTCAAGACCT TATGCCAGTA TAAATTTGAT TTTCTTCAAG AAGTATGTCA GCATGAACAT 
TTTATTCCTT TGTGTCTCCC CATAAGATCA GCAAACATTC CAGATCCTCT GACACCTTCG 
GAATCAACTC AAGAGCTACA TGCATCAGAT ATGCCTGAAT ATTCGGTCAC GAATGAATTT 
TGCCGCAAGC ACTTCTTAAT CGGGATTCTG CTCCGAGAAG TTGGCTTTGC TCTGCAGGAA 
GACCAAGATA TCAGGCACTT AGCTTTAGCT GTCCTGAAAA ATCTAATGGC TAAGCATTCG 
TTTGATGATC GATACAGAGA ACCAAGAAAG CAGGCCCAGA TAGCAAGCTT ATACATGCCC 
CTCTATGGCA TGCTTCTGGA CAACATGCCC AGGATTTACC TGAAGGATCT ATATCCTTTC 
ACTATCAATA CATCAAACCA GGGGTCTAGA GATGACCTCA GCACCAACGG AGGATTTCAC 
AGCCAGTCAG CTATGAAACA TGCAAACTCT GTGGACACAT CATTTTCCAA AGATGTTTTA 
AATTCCATAG CAGCATTTTC ATCAATAGCT ATTTCTACAG TAAACCATGC TGATTCCAGA 
GCATCCTTAG CAAGTCTGGA CTCCAATCCA AGTACCAATG AGAAGAGCAG TGAGAAAACT 
GACAACTGTG AAAAGATCCC AAGACCCTTG TCTTTGATAG GCTCAACGCT TCGATTTGAC 
AAGTTAGATC AAGCAGAAAC CAGGAGCCTT CTTATGTGCT TTCTTCAAAT TATGAAGACA 
ATTTCAGAGG AGACTCTGAT TGCCTACTGG CAAAGAGCAC CTAGTCCGGA AGTGTCTGAC 
TTCTTCAGCA TCTTGGATGT TTGTCTTCAA AATTTCAGAT ACCTAGGAAA ACGCAATATA 
ATAAGGAAAA TTGCTGCTGC ATTTAAATTT GTGCAGTCCA CCCAGAACAA CGGAACTCTC 
AAAGGATCCA ATCCTTCTTG CCAAACATCG GGTCTCTTGA CACAATGGAT GAACACCACT 
TCTGGTCACG AAGGACATAA GCAGCACAGA TCACAAACTT TACCTATAAT TCGAGGCAAA 
AATGCACTCT CTAACCCCAA ACTCTTACAG ATGCTAGACA ATACCATGAC CAGCAACTCC 
AATGAAATAG ACATCGTGCA CCACGTGGAC ACAGAGGCCA ATGTAGCTAC AGAGGTTTGC 
CTTACTATTC TAGACCTGCT GTCCCTCTTC ACTCAGATTC ACCATAGGCA ACTCCAACAA 
TCTGAATGTC AAAATTCATT GATGAAAAGG GTCTTTGATA CCTACATGCT CTTCTTCCAA 
GTCAACCAGT CAGCCACAGC ACTGAAGCAT GTGTTTGCTT CCCTGAGACT GTTTGTATGC 
AAGTTTCCCG CAGCGTTCTT TCAAGGGCCT GCTGACCTGT GTGGATCATT CTGTTACGAA 
GTCCTAAAAT GCTGTAACCA CAGGTCACGG TCAACCCAGA CAGAAGCATC GGCACTTCTA 
TACTTTTTCA TGAGGAAGAA TTTTGAATTT AATAAGCAGA AGTCAATTGT CCGATCCCAC 
CTACAGCTCA TCAAGGCTGT AAGCCAGTTA ATAGCCGATG CTGGGATTGG AGGCTCTCGG 
TTTCAACATT CCCTTGCAAT TACCAATAAT TTTGCTAATG GAGACAAGCA AATGAAAAAC 
AGCAATTTCC CAGCAGAGGT GAAAGATCTG ACTAAACGTA TAAGGACTGT TCTGATGGCC 
ACGGCTCAGA TGAAGGAGCA TGAGAAGGAC CCCGAGATGC TGGTGGACCT CCAGTATAGC 
CTGGCAAACT CCTATGCGAG CACCCCTGAG CTACGGAGGA CTTGGCTGGA AAGCATGGCC 
AAGATTCACG CAAGAAATGG AGACTTGTCC GAGGCTGCCA TGTGCTACAT CCACATAGCA 
GCCCTCATCG CAGAATACCT GAAAAGAAAG GGTTACTGGA AAATGGAAAA GATTTGCACG 
CCATCCCTGC TCCTGGAGGA TATCCATCCC TGTGATAGCA TCCCATTACT AACAACTCCC 
AGTGGAGGAA GCATGTTCTC CATGGGCTGG CCAGCTTTTC TGAGCATTAC ACCAAATATT 
AAAGAAGAAG GAGCAATGAA AGAAGATTCG GGAATGCAAG ACACACCATA CAATGAGAAT 
ATCCTGGTGG AACAGCTGTA TATGTGTGTG GAGTTTCTTT GGAAGTCTGA GAGATATGAA 
CTCATCGCTG ATGTCAACAA ACCCATCATC GCTGTCTTTG AGAAGCAGAG AGACTTCAAA 
AAATTGTCAG ATCTCTACTA TGACATTCAC CGGTCATACC TGAAAGTCGC AGAGGTGGTG 
AATTCAGAGA AGCGACTGTT TGGTCGGTAC TATCGCGTGG CGTTTTACGG GCAGGGGTTT 
TTTGAAGAAG AAGAAGGTAA AGAGTATATT TATAAAGAGC CTAAGCTGAC AGGTCTCTCC 
GAGATTTCCC AAAGACTCCT CAAGCTGTAT GCAGATAAAT TCGGAGCAGA CAATGTGAAG 
ATAATCCAGG ATTCCAACAA GGTAAACCCG AAGGACTTGG ACCCCAAATA TGCCTACATC 
CAGGTGACCT ACGTCACTCC ATTCTTTGAG GAAAAGGAAA TAGAGGACCG GAAGACGGAT 
TTCGAAATGC ACCACAACAT CAAGCGCTTT GTCTTTGAGA CGCCCTTCAC GCTGTCGGGC 
AAGAAGCATG GTGGCGTGGA GGAGCAGTGC AAGCGACGGA CGGTGCTGAC CACAAGTCAC 
CTGTTCCCCT ACGTGAAGAA GAGAATACAG GTCATAAGCC AGTCGAGCAC AGAACTGAAT 
CCTATTGAGG TGGCGATTGA TGAGATGTCC AAGAAAGTTT CTGAGCTTAA TCAGCTCTGC 
ACGATGGAGG AAGTGGACAT GATCAGACTG CAGCTCAAAC TCCAAGGAAG TGTCAGCGTG 
AAGGTTAATG CTGGACCAAT GGCCTATGCG CGAGCTTTTC TTGAAGAAAC CAACGCAAAG 
AAATACCCTG ACAACCAAGT AAAGCTTTTG AAGGAAATCT TCAGGCAATT TGCAGATGCG 
TGTGGGCAGG CCCTGGACGT GAATGAGCGC CTCATCAAGG AGGACCAGCT GGAGTACCAG 
GAAGAACTGA GGTCTCATTA TAAGGACATG CTCGGCGAAC TCTCTGCAAT TATGAATGAG 
CAGATTACGT ACAGGGACGA CCTGGCAAAG CGCGGATTGG AACGAACCTT CACTCGAGTA 
ACTAGCAAGG CAACTGCAGC TCCGCCCACG GTGTCCATCT CACCTTGCGT GGAGATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_023532931.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_023388699.1 Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X3, mRNA.

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CloneID	OPt70925
Clone ID Related Accession (Same CDS sequence)	XM_023532931.1
Accession Version	XM_023532931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Pteropus vampyrus(large flying fox)
Product	dedicator of cytokinesis protein 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011888874.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCA CCGCCAGCAT TGTTCTGCTA CAAGCTTTTC GTTCTGTGGT CCAGAGGAGC 
TGTCCACACA TTTGCAGCCG ACGCCAGGAG GAACCATCCC ATGAAATTTT GCCTCTAGAA 
AGTGACGATG AAATGAGCAG ACCCAGAACC CTCATCATAA TGCAGGAAAA GCCCAGGCTC 
CTTGAACCCT TGGATTATGA AACTGTCATT GAAGAACTTG AAAAGACCTA TCAGAATGAC 
CCTCTTCAAG ATCTCCTGTT CTTCCCCAGT GACGACTTTT CAACGGCCAC AGTTCCTTGG 
GACATTCGAA CACTGTACTC TACGGTACCT GAAGACGCAG AGCACAAAGC AGAAAATTTA 
CTGGTCAAAG AGGCTTGTAA ATTTTATAGT TCCCAGTGGT ACGTGGTAAA CTACAAATAT 
GAACAATATT CTGGAGATAT TCGCCAGTTA CCCCGAGCAG AATACAAACC AGAGAAACTC 
CCTTCGCATT CCTTTGAGGT TGACCATGAA GATGCTGATA AGGATGAAGA TACCACCTCC 
CACTCATCTT CCAAGGGCGG TGGGGGAGCA GGGGGAACTG GAGTCTTCAA GTCAGGCTGG 
CTCTACAAGG GGAATTTCAA CAGCACCGTG AACAATACCA TCACTGTTCG GTCATTCAAG 
AAGCGCTACT TCCAGCTGAC TCAGCTGCCA GATAACTCCT ACATCATGAA TTTTTACAAA 
GATGAAAAAA TATCCAAAGA GCCCAAAGGC TGCATCTTTT TGGATTCCTG TACAGGTGTG 
GTGCAGAATA ACAGACTAAG AAAATATGCC TTTGAATTGA AAATGAATGA CCTGACCTAT 
TTTGTGCTGG CGGCGGAAAC AGAGTGCGAC ATGGAGGAAT GGATCCACAC CCTGAACCGC 
ATCCTGCAAA TCAGCCCTGA GGGGCCCCTG CAGGGTCGAC GGAGCGCGGA GCTCACGGAT 
CTGGGCCTGG ATTCACTAGA TAATTCTATC ACATGTGAAT GCGCACCAGA GGAAACAGAT 
TCTTCAGAGA ACAGCCTACA TCCAGACTTC GCAAAATACC TCACAGAAAC TGAAGAGACT 
GTCAAAGCAA CTCGAAACAT GGAGAGACTC AATCTGTTCT CGATGGACCC AGATATAGAT 
ACCTTGAAAC TTCAGAAAAA GGATCTTTTG GAATCCGAGT CTGTGATCAA ACCATTTGAA 
GAAAAAGCTG CCAAGAGAAT CATGATCATA TGTAAAGCTC TCACCTTCAG TCTTCAGGGA 
TGTGTTACAG AGAATGAAAA TGATCCCATA ACCAACATTG AGCCTTTTTT TGTGAGTGTG 
GCACTTTATG ACCTCAGAAA CAGCAGGAAG ATTTCTGCCG ACTTCCACGT GGATCTAAAC 
CACACTGCTG TCAGGCAGAT GCTCTCAGGG GCTCCCGTGG CTTTGGAAAA TGGAAACAGC 
GACACGGTCA CCCCAAGACA ATCAGAAGAA CCTCACGTCA AGGGGTTTCC AGAGGAATGG 
CTGAAATTTC CAAAGCAGGC TATATTTTCT GTAAGCAATC CACATTCGGA AATTGTCTTG 
GTGGCTAAAA TTGAAAAAGT ACTAATGGGA AACATTGCAA GTGGTGCTGA ACCTTACATT 
AAGAACCCAG ACTCCAACAA GTATGCACAA AAGATACTCA AATCCAACAG ACAGTTCTGC 
AGCAAGTTGG GGAAATACCG CATGCCGTTT GCTTGGGCGA TGAGATCAGT TTTTAAGGAC 
AACCAGGGAA ATGTGGACAG AGACTCAAGA TTTTCACCAT TGTATAGACA CGAAAGTAGC 
AAGATTTCAA CTGAGGACCT ACTTAAACTA GTGTCAGATT ATAGGAGGGC CGACAGAATA 
AGCAAAATGC AGACCATTCC AGGAAGCCTC GATATCACTG TTGAGAACAT TCCTCTGGAG 
CATCCAAACT GTGTAACATC GTCCTTTATC CCCGTCAAGC CTTTCAACGT GACGGCTCAA 
CCGGAACCCA CAGTGGAGGT GGAAGAATTT GTTTATGACT CAACAAAGTA CTGCCGTCCT 
TACAGAGTAT ACAAAAATCA AATTTACATT TACCCCAAGC ACCTCAAGTA TGATAGCCAG 
AAATGTTTCA ACAAGGCACG AAATATAACC GTGTGCATTG AATTCAAAAA TTCTGACGAC 
GAAGGTGCCA AGCCCATGAA GTGTATTTAT GGGAAGCCTG GAGGGCCCCT CTTCACCTCA 
GCCGCCTACA CAGCAGTTCT GCACCATTCC CAGAATCCGG ATTTCTCAGA CGAGGTGAAA 
ATTGAGCTAC CAACACAACT CCACGAGAAA CACCATATTC TGTTTTCTTT TTATCATGTC 
ACTTGCGACA TAAATGCAAA AGCTAATGCC AAAAAGAAGG AAGTTCTGGA AACATCAGTT 
GGATATGCTT GGCTTCCTCT AATGAAACAT GATCAGATAG CTTCTCAAGA GTACAATATC 
CCAATAGCTA CAAGCCTGCC TCCTAATTAT TTAAGCTTTC AAGATTCTGC AAGTGGAAAG 
CATGGTGGGA GTGACATTAA ATGGGTTGAT GGTGGCAAAC CACTTTTCAA AGTATCAACA 
TTCGTTGTAT CAACAGTGAA TACTCAGGAT CCGTATGTGA ACGCGTTTTT CCGACAGTGC 
CAAAAAAGAG AGAAAGATAT GTCTCAGTCA CCTACCTCGA ATTTCATCCG CTCTTGTAAG 
AACTTATTGA ATGTGGAAAA AATTCACGCA ATCATGAGTT TTCTGCCTAT AATCTTGAAT 
CAGCTCTTCA AGGTTTTGGT ACAGAATGAG GAAGATGAAA TAAGTACAAC TGTGACCAGG 
GTTCTGACTG ACATTGTGGC CAAGTGCCAC GAGGAGCAGC TGGACCACTC CGTCCAGTCA 
TACATTAAGT TTGTGTTCAA GACCAAGGCT TGCAAGGAGA GAACGATACA CGAGGAACTG 
ACTAAGAGTG TGACTGGTCT TTTGAAATCA AATGACTCCA CAACAGTCAG GCACGTTCTG 
AAGCATTCCT GGTTCTTCTT CGCAATTATT CTAAAATCGA TGGCACAACA CTTGATTGAT 
ACAAATAAAA TTCAGCTTTC TCGGGCTCAA AGATTTCCTG AATCTTACCA GAATGAATTG 
GACAATCTTA TAATGGTCCT ATCTGATCAC GTGATCTGGA AATACAAAGA TGCCCTTGAA 
GAAACAAGAA GGGCCAACCA CAGCGTTGCC AGATTTCTCA AGCGCTGCTT CACCTTCATG 
GATCGGGGCT TTGTGTTTAA GATGGTTAAC AATTACATCA GCATGTTCTC CTCTGGTGAG 
CTCAAGACCT TATGCCAGTA TAAATTTGAT TTTCTTCAAG AAGTATGTCA GCATGAACAT 
TTTATTCCTT TGTGTCTCCC CATAAGATCA GCAAACATTC CAGATCCTCT GACACCTTCG 
GAATCAACTC AAGAGCTACA TGCATCAGAT ATGCCTGAAT ATTCGGTCAC GAATGAATTT 
TGCCGCAAGC ACTTCTTAAT CGGGATTCTG CTCCGAGAAG TTGGCTTTGC TCTGCAGGAA 
GACCAAGATA TCAGGCACTT AGCTTTAGCT GTCCTGAAAA ATCTAATGGC TAAGCATTCG 
TTTGATGATC GATACAGAGA ACCAAGAAAG CAGGCCCAGA TAGCAAGCTT ATACATGCCC 
CTCTATGGCA TGCTTCTGGA CAACATGCCC AGGATTTACC TGAAGGATCT ATATCCTTTC 
ACTATCAATA CATCAAACCA GGGGTCTAGA GATGACCTCA GCACCAACGG AGGATTTCAC 
AGCCAGTCAG CTATGAAACA TGCAAACTCT GTGGACACAT CATTTTCCAA AGATGTTTTA 
AATTCCATAG CAGTAAACCA TGCTGATTCC AGAGCATCCT TAGCAAGTCT GGACTCCAAT 
CCAAGTACCA ATGAGAAGAG CAGTGAGAAA ACTGACAACT GTGAAAAGAT CCCAAGACCC 
TTGTCTTTGA TAGGCTCAAC GCTTCGATTT GACAAGTTAG ATCAAGCAGA AACCAGGAGC 
CTTCTTATGT GCTTTCTTCA AATTATGAAG ACAATTTCAG AGGAGACTCT GATTGCCTAC 
TGGCAAAGAG CACCTAGTCC GGAAGTGTCT GACTTCTTCA GCATCTTGGA TGTTTGTCTT 
CAAAATTTCA GATACCTAGG AAAACGCAAT ATAATAAGGA AAATTGCTGC TGCATTTAAA 
TTTGTGCAGT CCACCCAGAA CAACGGAACT CTCAAAGGAT CCAATCCTTC TTGCCAAACA 
TCGGGTCTCT TGACACAATG GATGAACACC ACTTCTGGTC ACGAAGGACA TAAGCAGCAC 
AGATCACAAA CTTTACCTAT AATTCGAGGC AAAAATGCAC TCTCTAACCC CAAACTCTTA 
CAGATGCTAG ACAATACCAT GACCAGCAAC TCCAATGAAA TAGACATCGT GCACCACGTG 
GACACAGAGG CCAATGTAGC TACAGAGGTT TGCCTTACTA TTCTAGACCT GCTGTCCCTC 
TTCACTCAGA TTCACCATAG GCAACTCCAA CAATCTGAAT GTCAAAATTC ATTGATGAAA 
AGGGTCTTTG ATACCTACAT GCTCTTCTTC CAAGTCAACC AGTCAGCCAC AGCACTGAAG 
CATGTGTTTG CTTCCCTGAG ACTGTTTGTA TGCAAGTTTC CCGCAGCGTT CTTTCAAGGG 
CCTGCTGACC TGTGTGGATC ATTCTGTTAC GAAGTCCTAA AATGCTGTAA CCACAGGTCA 
CGGTCAACCC AGACAGAAGC ATCGGCACTT CTATACTTTT TCATGAGGAA GAATTTTGAA 
TTTAATAAGC AGAAGTCAAT TGTCCGATCC CACCTACAGC TCATCAAGGC TGTAAGCCAG 
TTAATAGCCG ATGCTGGGAT TGGAGGCTCT CGGTTTCAAC ATTCCCTTGC AATTACCAAT 
AATTTTGCTA ATGGAGACAA GCAAATGAAA AACAGCAATT TCCCAGCAGA GGTGAAAGAT 
CTGACTAAAC GTATAAGGAC TGTTCTGATG GCCACGGCTC AGATGAAGGA GCATGAGAAG 
GACCCCGAGA TGCTGGTGGA CCTCCAGTAT AGCCTGGCAA ACTCCTATGC GAGCACCCCT 
GAGCTACGGA GGACTTGGCT GGAAAGCATG GCCAAGATTC ACGCAAGAAA TGGAGACTTG 
TCCGAGGCTG CCATGTGCTA CATCCACATA GCAGCCCTCA TCGCAGAATA CCTGAAAAGA 
AAGGGTTACT GGAAAATGGA AAAGATTTGC ACGCCATCCC TGCTCCTGGA GGATATCCAT 
CCCTGTGATA GCATCCCATT ACTAACAACT CCCAGTGGAG GAAGCATGTT CTCCATGGGC 
TGGCCAGCTT TTCTGAGCAT TACACCAAAT ATTAAAGAAG AAGGAGCAAT GAAAGAAGAT 
TCGGGAATGC AAGACACACC ATACAATGAG AATATCCTGG TGGAACAGCT GTATATGTGT 
GTGGAGTTTC TTTGGAAGTC TGAGAGATAT GAACTCATCG CTGATGTCAA CAAACCCATC 
ATCGCTGTCT TTGAGAAGCA GAGAGACTTC AAAAAATTGT CAGATCTCTA CTATGACATT 
CACCGGTCAT ACCTGAAAGT CGCAGAGGTG GTGAATTCAG AGAAGCGACT GTTTGGTCGG 
TACTATCGCG TGGCGTTTTA CGGGCAGGGG TTTTTTGAAG AAGAAGAAGG TAAAGAGTAT 
ATTTATAAAG AGCCTAAGCT GACAGGTCTC TCCGAGATTT CCCAAAGACT CCTCAAGCTG 
TATGCAGATA AATTCGGAGC AGACAATGTG AAGATAATCC AGGATTCCAA CAAGGTAAAC 
CCGAAGGACT TGGACCCCAA ATATGCCTAC ATCCAGGTGA CCTACGTCAC TCCATTCTTT 
GAGGAAAAGG AAATAGAGGA CCGGAAGACG GATTTCGAAA TGCACCACAA CATCAAGCGC 
TTTGTCTTTG AGACGCCCTT CACGCTGTCG GGCAAGAAGC ATGGTGGCGT GGAGGAGCAG 
TGCAAGCGAC GGACGGTGCT GACCACAAGT CACCTGTTCC CCTACGTGAA GAAGAGAATA 
CAGGTCATAA GCCAGTCGAG CACAGAACTG AATCCTATTG AGGTGGCGAT TGATGAGATG 
TCCAAGAAAG TTTCTGAGCT TAATCAGCTC TGCACGATGG AGGAAGTGGA CATGATCAGA 
CTGCAGCTCA AACTCCAAGG AAGTGTCAGC GTGAAGGTTA ATGCTGGACC AATGGCCTAT 
GCGCGAGCTT TTCTTGAAGA AACCAACGCA AAGAAATACC CTGACAACCA AGTAAAGCTT 
TTGAAGGAAA TCTTCAGGCA ATTTGCAGAT GCGTGTGGGC AGGCCCTGGA CGTGAATGAG 
CGCCTCATCA AGGAGGACCA GCTGGAGTAC CAGGAAGAAC TGAGGTCTCA TTATAAGGAC 
ATGCTCGGCG AACTCTCTGC AATTATGAAT GAGCAGATTA CGTACAGGGA CGACCTGGCA 
AAGCGCGGAT TGGAACGAAC CTTCACTCGA GTAACTAGCA AGGCAACTGC AGCTCCGCCC 
ACGGTGTCCA TCTCACCTTG CGTGGAGATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023388699.1
CDS	16..6588
Translation	MGCTASIVLLQAFRSVVQRSCPHICSRRQEEPSHEILPLESDDEMSRPRTLIIMQEKPRLLEPLDYETVIEELEKTYQNDPLQDLLFFPSDDFSTATVPWDIRTLYSTVPEDAEHKAENLLVKEACKFYSSQWYVVNYKYEQYSGDIRQLPRAEYKPEKLPSHSFEVDHEDADKDEDTTSHSSSKGGGGAGGTGVFKSGWLYKGNFNSTVNNTITVRSFKKRYFQLTQLPDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKYAFELKMNDLTYFVLAAETECDMEEWIHTLNRILQISPEGPLQGRRSAELTDLGLDSLDNSITCECAPEETDSSENSLHPDFAKYLTETEETVKATRNMERLNLFSMDPDIDTLKLQKKDLLESESVIKPFEEKAAKRIMIICKALTFSLQGCVTENENDPITNIEPFFVSVALYDLRNSRKISADFHVDLNHTAVRQMLSGAPVALENGNSDTVTPRQSEEPHVKGFPEEWLKFPKQAIFSVSNPHSEIVLVAKIEKVLMGNIASGAEPYIKNPDSNKYAQKILKSNRQFCSKLGKYRMPFAWAMRSVFKDNQGNVDRDSRFSPLYRHESSKISTEDLLKLVSDYRRADRISKMQTIPGSLDITVENIPLEHPNCVTSSFIPVKPFNVTAQPEPTVEVEEFVYDSTKYCRPYRVYKNQIYIYPKHLKYDSQKCFNKARNITVCIEFKNSDDEGAKPMKCIYGKPGGPLFTSAAYTAVLHHSQNPDFSDEVKIELPTQLHEKHHILFSFYHVTCDINAKANAKKKEVLETSVGYAWLPLMKHDQIASQEYNIPIATSLPPNYLSFQDSASGKHGGSDIKWVDGGKPLFKVSTFVVSTVNTQDPYVNAFFRQCQKREKDMSQSPTSNFIRSCKNLLNVEKIHAIMSFLPIILNQLFKVLVQNEEDEISTTVTRVLTDIVAKCHEEQLDHSVQSYIKFVFKTKACKERTIHEELTKSVTGLLKSNDSTTVRHVLKHSWFFFAIILKSMAQHLIDTNKIQLSRAQRFPESYQNELDNLIMVLSDHVIWKYKDALEETRRANHSVARFLKRCFTFMDRGFVFKMVNNYISMFSSGELKTLCQYKFDFLQEVCQHEHFIPLCLPIRSANIPDPLTPSESTQELHASDMPEYSVTNEFCRKHFLIGILLREVGFALQEDQDIRHLALAVLKNLMAKHSFDDRYREPRKQAQIASLYMPLYGMLLDNMPRIYLKDLYPFTINTSNQGSRDDLSTNGGFHSQSAMKHANSVDTSFSKDVLNSIAVNHADSRASLASLDSNPSTNEKSSEKTDNCEKIPRPLSLIGSTLRFDKLDQAETRSLLMCFLQIMKTISEETLIAYWQRAPSPEVSDFFSILDVCLQNFRYLGKRNIIRKIAAAFKFVQSTQNNGTLKGSNPSCQTSGLLTQWMNTTSGHEGHKQHRSQTLPIIRGKNALSNPKLLQMLDNTMTSNSNEIDIVHHVDTEANVATEVCLTILDLLSLFTQIHHRQLQQSECQNSLMKRVFDTYMLFFQVNQSATALKHVFASLRLFVCKFPAAFFQGPADLCGSFCYEVLKCCNHRSRSTQTEASALLYFFMRKNFEFNKQKSIVRSHLQLIKAVSQLIADAGIGGSRFQHSLAITNNFANGDKQMKNSNFPAEVKDLTKRIRTVLMATAQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHARNGDLSEAAMCYIHIAALIAEYLKRKGYWKMEKICTPSLLLEDIHPCDSIPLLTTPSGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQDTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNSEKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVNPKDLDPKYAYIQVTYVTPFFEEKEIEDRKTDFEMHHNIKRFVFETPFTLSGKKHGGVEEQCKRRTVLTTSHLFPYVKKRIQVISQSSTELNPIEVAIDEMSKKVSELNQLCTMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEETNAKKYPDNQVKLLKEIFRQFADACGQALDVNERLIKEDQLEYQEELRSHYKDMLGELSAIMNEQITYRDDLAKRGLERTFTRVTSKATAAPPTVSISPCVEI

Target ORF information:

RefSeq Version	XM_023532931.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus dedicator of cytokinesis 10 (DOCK10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023532931.1

ORF Insert Sequence:

ATGGGCTGCA CCGCCAGCAT TGTTCTGCTA CAAGCTTTTC GTTCTGTGGT CCAGAGGAGC 
TGTCCACACA TTTGCAGCCG ACGCCAGGAG GAACCATCCC ATGAAATTTT GCCTCTAGAA 
AGTGACGATG AAATGAGCAG ACCCAGAACC CTCATCATAA TGCAGGAAAA GCCCAGGCTC 
CTTGAACCCT TGGATTATGA AACTGTCATT GAAGAACTTG AAAAGACCTA TCAGAATGAC 
CCTCTTCAAG ATCTCCTGTT CTTCCCCAGT GACGACTTTT CAACGGCCAC AGTTCCTTGG 
GACATTCGAA CACTGTACTC TACGGTACCT GAAGACGCAG AGCACAAAGC AGAAAATTTA 
CTGGTCAAAG AGGCTTGTAA ATTTTATAGT TCCCAGTGGT ACGTGGTAAA CTACAAATAT 
GAACAATATT CTGGAGATAT TCGCCAGTTA CCCCGAGCAG AATACAAACC AGAGAAACTC 
CCTTCGCATT CCTTTGAGGT TGACCATGAA GATGCTGATA AGGATGAAGA TACCACCTCC 
CACTCATCTT CCAAGGGCGG TGGGGGAGCA GGGGGAACTG GAGTCTTCAA GTCAGGCTGG 
CTCTACAAGG GGAATTTCAA CAGCACCGTG AACAATACCA TCACTGTTCG GTCATTCAAG 
AAGCGCTACT TCCAGCTGAC TCAGCTGCCA GATAACTCCT ACATCATGAA TTTTTACAAA 
GATGAAAAAA TATCCAAAGA GCCCAAAGGC TGCATCTTTT TGGATTCCTG TACAGGTGTG 
GTGCAGAATA ACAGACTAAG AAAATATGCC TTTGAATTGA AAATGAATGA CCTGACCTAT 
TTTGTGCTGG CGGCGGAAAC AGAGTGCGAC ATGGAGGAAT GGATCCACAC CCTGAACCGC 
ATCCTGCAAA TCAGCCCTGA GGGGCCCCTG CAGGGTCGAC GGAGCGCGGA GCTCACGGAT 
CTGGGCCTGG ATTCACTAGA TAATTCTATC ACATGTGAAT GCGCACCAGA GGAAACAGAT 
TCTTCAGAGA ACAGCCTACA TCCAGACTTC GCAAAATACC TCACAGAAAC TGAAGAGACT 
GTCAAAGCAA CTCGAAACAT GGAGAGACTC AATCTGTTCT CGATGGACCC AGATATAGAT 
ACCTTGAAAC TTCAGAAAAA GGATCTTTTG GAATCCGAGT CTGTGATCAA ACCATTTGAA 
GAAAAAGCTG CCAAGAGAAT CATGATCATA TGTAAAGCTC TCACCTTCAG TCTTCAGGGA 
TGTGTTACAG AGAATGAAAA TGATCCCATA ACCAACATTG AGCCTTTTTT TGTGAGTGTG 
GCACTTTATG ACCTCAGAAA CAGCAGGAAG ATTTCTGCCG ACTTCCACGT GGATCTAAAC 
CACACTGCTG TCAGGCAGAT GCTCTCAGGG GCTCCCGTGG CTTTGGAAAA TGGAAACAGC 
GACACGGTCA CCCCAAGACA ATCAGAAGAA CCTCACGTCA AGGGGTTTCC AGAGGAATGG 
CTGAAATTTC CAAAGCAGGC TATATTTTCT GTAAGCAATC CACATTCGGA AATTGTCTTG 
GTGGCTAAAA TTGAAAAAGT ACTAATGGGA AACATTGCAA GTGGTGCTGA ACCTTACATT 
AAGAACCCAG ACTCCAACAA GTATGCACAA AAGATACTCA AATCCAACAG ACAGTTCTGC 
AGCAAGTTGG GGAAATACCG CATGCCGTTT GCTTGGGCGA TGAGATCAGT TTTTAAGGAC 
AACCAGGGAA ATGTGGACAG AGACTCAAGA TTTTCACCAT TGTATAGACA CGAAAGTAGC 
AAGATTTCAA CTGAGGACCT ACTTAAACTA GTGTCAGATT ATAGGAGGGC CGACAGAATA 
AGCAAAATGC AGACCATTCC AGGAAGCCTC GATATCACTG TTGAGAACAT TCCTCTGGAG 
CATCCAAACT GTGTAACATC GTCCTTTATC CCCGTCAAGC CTTTCAACGT GACGGCTCAA 
CCGGAACCCA CAGTGGAGGT GGAAGAATTT GTTTATGACT CAACAAAGTA CTGCCGTCCT 
TACAGAGTAT ACAAAAATCA AATTTACATT TACCCCAAGC ACCTCAAGTA TGATAGCCAG 
AAATGTTTCA ACAAGGCACG AAATATAACC GTGTGCATTG AATTCAAAAA TTCTGACGAC 
GAAGGTGCCA AGCCCATGAA GTGTATTTAT GGGAAGCCTG GAGGGCCCCT CTTCACCTCA 
GCCGCCTACA CAGCAGTTCT GCACCATTCC CAGAATCCGG ATTTCTCAGA CGAGGTGAAA 
ATTGAGCTAC CAACACAACT CCACGAGAAA CACCATATTC TGTTTTCTTT TTATCATGTC 
ACTTGCGACA TAAATGCAAA AGCTAATGCC AAAAAGAAGG AAGTTCTGGA AACATCAGTT 
GGATATGCTT GGCTTCCTCT AATGAAACAT GATCAGATAG CTTCTCAAGA GTACAATATC 
CCAATAGCTA CAAGCCTGCC TCCTAATTAT TTAAGCTTTC AAGATTCTGC AAGTGGAAAG 
CATGGTGGGA GTGACATTAA ATGGGTTGAT GGTGGCAAAC CACTTTTCAA AGTATCAACA 
TTCGTTGTAT CAACAGTGAA TACTCAGGAT CCGTATGTGA ACGCGTTTTT CCGACAGTGC 
CAAAAAAGAG AGAAAGATAT GTCTCAGTCA CCTACCTCGA ATTTCATCCG CTCTTGTAAG 
AACTTATTGA ATGTGGAAAA AATTCACGCA ATCATGAGTT TTCTGCCTAT AATCTTGAAT 
CAGCTCTTCA AGGTTTTGGT ACAGAATGAG GAAGATGAAA TAAGTACAAC TGTGACCAGG 
GTTCTGACTG ACATTGTGGC CAAGTGCCAC GAGGAGCAGC TGGACCACTC CGTCCAGTCA 
TACATTAAGT TTGTGTTCAA GACCAAGGCT TGCAAGGAGA GAACGATACA CGAGGAACTG 
ACTAAGAGTG TGACTGGTCT TTTGAAATCA AATGACTCCA CAACAGTCAG GCACGTTCTG 
AAGCATTCCT GGTTCTTCTT CGCAATTATT CTAAAATCGA TGGCACAACA CTTGATTGAT 
ACAAATAAAA TTCAGCTTTC TCGGGCTCAA AGATTTCCTG AATCTTACCA GAATGAATTG 
GACAATCTTA TAATGGTCCT ATCTGATCAC GTGATCTGGA AATACAAAGA TGCCCTTGAA 
GAAACAAGAA GGGCCAACCA CAGCGTTGCC AGATTTCTCA AGCGCTGCTT CACCTTCATG 
GATCGGGGCT TTGTGTTTAA GATGGTTAAC AATTACATCA GCATGTTCTC CTCTGGTGAG 
CTCAAGACCT TATGCCAGTA TAAATTTGAT TTTCTTCAAG AAGTATGTCA GCATGAACAT 
TTTATTCCTT TGTGTCTCCC CATAAGATCA GCAAACATTC CAGATCCTCT GACACCTTCG 
GAATCAACTC AAGAGCTACA TGCATCAGAT ATGCCTGAAT ATTCGGTCAC GAATGAATTT 
TGCCGCAAGC ACTTCTTAAT CGGGATTCTG CTCCGAGAAG TTGGCTTTGC TCTGCAGGAA 
GACCAAGATA TCAGGCACTT AGCTTTAGCT GTCCTGAAAA ATCTAATGGC TAAGCATTCG 
TTTGATGATC GATACAGAGA ACCAAGAAAG CAGGCCCAGA TAGCAAGCTT ATACATGCCC 
CTCTATGGCA TGCTTCTGGA CAACATGCCC AGGATTTACC TGAAGGATCT ATATCCTTTC 
ACTATCAATA CATCAAACCA GGGGTCTAGA GATGACCTCA GCACCAACGG AGGATTTCAC 
AGCCAGTCAG CTATGAAACA TGCAAACTCT GTGGACACAT CATTTTCCAA AGATGTTTTA 
AATTCCATAG CAGTAAACCA TGCTGATTCC AGAGCATCCT TAGCAAGTCT GGACTCCAAT 
CCAAGTACCA ATGAGAAGAG CAGTGAGAAA ACTGACAACT GTGAAAAGAT CCCAAGACCC 
TTGTCTTTGA TAGGCTCAAC GCTTCGATTT GACAAGTTAG ATCAAGCAGA AACCAGGAGC 
CTTCTTATGT GCTTTCTTCA AATTATGAAG ACAATTTCAG AGGAGACTCT GATTGCCTAC 
TGGCAAAGAG CACCTAGTCC GGAAGTGTCT GACTTCTTCA GCATCTTGGA TGTTTGTCTT 
CAAAATTTCA GATACCTAGG AAAACGCAAT ATAATAAGGA AAATTGCTGC TGCATTTAAA 
TTTGTGCAGT CCACCCAGAA CAACGGAACT CTCAAAGGAT CCAATCCTTC TTGCCAAACA 
TCGGGTCTCT TGACACAATG GATGAACACC ACTTCTGGTC ACGAAGGACA TAAGCAGCAC 
AGATCACAAA CTTTACCTAT AATTCGAGGC AAAAATGCAC TCTCTAACCC CAAACTCTTA 
CAGATGCTAG ACAATACCAT GACCAGCAAC TCCAATGAAA TAGACATCGT GCACCACGTG 
GACACAGAGG CCAATGTAGC TACAGAGGTT TGCCTTACTA TTCTAGACCT GCTGTCCCTC 
TTCACTCAGA TTCACCATAG GCAACTCCAA CAATCTGAAT GTCAAAATTC ATTGATGAAA 
AGGGTCTTTG ATACCTACAT GCTCTTCTTC CAAGTCAACC AGTCAGCCAC AGCACTGAAG 
CATGTGTTTG CTTCCCTGAG ACTGTTTGTA TGCAAGTTTC CCGCAGCGTT CTTTCAAGGG 
CCTGCTGACC TGTGTGGATC ATTCTGTTAC GAAGTCCTAA AATGCTGTAA CCACAGGTCA 
CGGTCAACCC AGACAGAAGC ATCGGCACTT CTATACTTTT TCATGAGGAA GAATTTTGAA 
TTTAATAAGC AGAAGTCAAT TGTCCGATCC CACCTACAGC TCATCAAGGC TGTAAGCCAG 
TTAATAGCCG ATGCTGGGAT TGGAGGCTCT CGGTTTCAAC ATTCCCTTGC AATTACCAAT 
AATTTTGCTA ATGGAGACAA GCAAATGAAA AACAGCAATT TCCCAGCAGA GGTGAAAGAT 
CTGACTAAAC GTATAAGGAC TGTTCTGATG GCCACGGCTC AGATGAAGGA GCATGAGAAG 
GACCCCGAGA TGCTGGTGGA CCTCCAGTAT AGCCTGGCAA ACTCCTATGC GAGCACCCCT 
GAGCTACGGA GGACTTGGCT GGAAAGCATG GCCAAGATTC ACGCAAGAAA TGGAGACTTG 
TCCGAGGCTG CCATGTGCTA CATCCACATA GCAGCCCTCA TCGCAGAATA CCTGAAAAGA 
AAGGGTTACT GGAAAATGGA AAAGATTTGC ACGCCATCCC TGCTCCTGGA GGATATCCAT 
CCCTGTGATA GCATCCCATT ACTAACAACT CCCAGTGGAG GAAGCATGTT CTCCATGGGC 
TGGCCAGCTT TTCTGAGCAT TACACCAAAT ATTAAAGAAG AAGGAGCAAT GAAAGAAGAT 
TCGGGAATGC AAGACACACC ATACAATGAG AATATCCTGG TGGAACAGCT GTATATGTGT 
GTGGAGTTTC TTTGGAAGTC TGAGAGATAT GAACTCATCG CTGATGTCAA CAAACCCATC 
ATCGCTGTCT TTGAGAAGCA GAGAGACTTC AAAAAATTGT CAGATCTCTA CTATGACATT 
CACCGGTCAT ACCTGAAAGT CGCAGAGGTG GTGAATTCAG AGAAGCGACT GTTTGGTCGG 
TACTATCGCG TGGCGTTTTA CGGGCAGGGG TTTTTTGAAG AAGAAGAAGG TAAAGAGTAT 
ATTTATAAAG AGCCTAAGCT GACAGGTCTC TCCGAGATTT CCCAAAGACT CCTCAAGCTG 
TATGCAGATA AATTCGGAGC AGACAATGTG AAGATAATCC AGGATTCCAA CAAGGTAAAC 
CCGAAGGACT TGGACCCCAA ATATGCCTAC ATCCAGGTGA CCTACGTCAC TCCATTCTTT 
GAGGAAAAGG AAATAGAGGA CCGGAAGACG GATTTCGAAA TGCACCACAA CATCAAGCGC 
TTTGTCTTTG AGACGCCCTT CACGCTGTCG GGCAAGAAGC ATGGTGGCGT GGAGGAGCAG 
TGCAAGCGAC GGACGGTGCT GACCACAAGT CACCTGTTCC CCTACGTGAA GAAGAGAATA 
CAGGTCATAA GCCAGTCGAG CACAGAACTG AATCCTATTG AGGTGGCGAT TGATGAGATG 
TCCAAGAAAG TTTCTGAGCT TAATCAGCTC TGCACGATGG AGGAAGTGGA CATGATCAGA 
CTGCAGCTCA AACTCCAAGG AAGTGTCAGC GTGAAGGTTA ATGCTGGACC AATGGCCTAT 
GCGCGAGCTT TTCTTGAAGA AACCAACGCA AAGAAATACC CTGACAACCA AGTAAAGCTT 
TTGAAGGAAA TCTTCAGGCA ATTTGCAGAT GCGTGTGGGC AGGCCCTGGA CGTGAATGAG 
CGCCTCATCA AGGAGGACCA GCTGGAGTAC CAGGAAGAAC TGAGGTCTCA TTATAAGGAC 
ATGCTCGGCG AACTCTCTGC AATTATGAAT GAGCAGATTA CGTACAGGGA CGACCTGGCA 
AAGCGCGGAT TGGAACGAAC CTTCACTCGA GTAACTAGCA AGGCAACTGC AGCTCCGCCC 
ACGGTGTCCA TCTCACCTTG CGTGGAGATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DOCK10 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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