PKH_122370 cDNA ORF clone, Plasmodium knowlesi strain H

Gene
Clones
gRNAs

Summary

Gene Symbol	PKH_122370
Entrez Gene ID	7322503
Full Name	DNA-directed RNA polymerase 3 largest subunit,putative
Gene Type	protein-coding
Organism	Plasmodium knowlesi strain H

mRNA and Protein(s)

mRNA	Protein	Name
XM_002260011.1	XP_002260047.1	DNA-directed RNA polymerase 3 largest subunit,putative

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the simian and human malaria parasite Plasmodium knowlesi.
Pain A, Böhme U, Berry AE, Mungall K, Finn RD, Jackson AP, Mourier T, Mistry J, Pasini EM, Aslett MA, Balasubrammaniam S, Borgwardt K, Brooks K, Carret C, Carver TJ, Cherevach I, Chillingworth T, Clark TG, Galinski MR, Hall N, Harper D, Harris D, Hauser H, Ivens A, Janssen CS, Keane T, Larke N, Lapp S, Marti M, Moule S, Meyer IM, Ormond D, Peters N, Sanders M, Sanders S, Sargeant TJ, Simmonds M, Smith F, Squares R, Thurston S, Tivey AR, Walker D, White B, Zuiderwijk E, Churcher C, Quail MA, Cowman AF, Turner CM, Rajandream MA, Kocken CH, Thomas AW, Newbold CI, Barrell BG, Berriman M
Nature455(7214)799-803(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PKH_122370 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKH_122370 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPs03093	XM_002260011.1 Latest version!	Plasmodium knowlesi strain H DNA-directed RNA polymerase 3 largest subunit,putative partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PKH_122370 ( XM_002260011.1 ) cDNA ORF clone, Plasmodium knowlesi strain H -> XP_002260047.1 Plasmodium knowlesi strain H DNA-directed RNA polymerase 3 largest subunit,putative partial mRNA.

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CloneID	OPs03093
Clone ID Related Accession (Same CDS sequence)	XM_002260011.1
Accession Version	XM_002260011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Plasmodium knowlesi strain H
Product	DNA-directed RNA polymerase 3 largest subunit,putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_011913). COMPLETENESS: incomplete on both ends.

ATGGATGAAT TGCGAGTAAA GTCCAACGAT GTGGAGGAGT TGCGGAGGAT AATTCAAAGG 
ACCAGCCTGC GGAAGGGATT CGTACAAGAT GTGAAGAGCA ACTGGGAAAT CAAAAGTCTC 
CAGTTCGGAA TTATGAACAA GGAAGAAATA ATAAAATGCT CAGAAGTGAA AATTTTAAAC 
AGAGAAATGT ACAAAAACAA CACAGGTATT CCCTACCCTT ATGGAGTCTT GGATCTCAAG 
TTAGGAGCTC ACAAAAGTAA CTCTCTGTGT GAAACATGTA ACAAAAATTT TATGAACTGT 
TCAGGTCATT TTGGATACAT TGAATTAAAC TACCCACTCT TTCATGTGGG GTACTACAAG 
TACATCATCT ATATCCTGTA CTGCATTTGC AAAGTCTGCT CACATATTTT ACTAACCAAT 
GAAAAGCTTG AATTTTATAC GAATCTGAAA AGACGTGCAT CGGAGGATAA GTTTTTCAAA 
AAACACATTT TTAAGAAAAT CTTAAACAGT TGTAAAAAGG TGACAAAATG CTACCGTTGT 
GGATATCCTC AAGGAGTGAT AAAAAAAATT ATAAAACCCA GTTTAGATCA ATTCATGAAA 
TTAAAACATG TAATTAAAAT CAAAGAAAAT GGTAAAATGG TAATAAAAGA GGATGATTTA 
AATAGTCTTT ACGTTTTAAA TCTCTTCAAA AATATGAATC CTTACCATGT GAAACTTTTA 
AATGTGGAAA ATCCAGAGAA GCTACTCATA ACGGCTTTGT TGGTACCTCC CAACACCATT 
AGACCATCAG TTATAATTGA TGAACATGGT ACAGCAGAGG ATGACTTGAC TTGTATTCTA 
TCAGAAATCA CACAACTTAA CAACACCATT AATAATCAGT GCAGTAATGG TTACCAGACT 
AATCAGTTCT TAGGTAATGT AGAATTTCTT CAACTTCAGA TTACAAGGTT CATCAATAGC 
GATTCCCCTG CTGTGTCTCA GTTATTAGCT ACTCAAAATA TTTCCAAACC AGGTAGAGGA 
ATCTGTCAGA GGCTGAAAGG AAAAGAAGGT AGATTCAGAT GTAACTTATC CGGTAAAAGG 
GTAGACTTTT CAAGCAGAAC TGTCATTTCA CCGGATCCTA ATATCTCCAT TGATGAGGTT 
GTTATTCCGG AAATTATCGC TATGAGGCTT ACGTACCCGG AGAAGGTTAA TCGGTACAAC 
ATTGAGAGGT TAAAGAAATT TATAAAGAAC GGAACAAACA CATGGCCTGG AGCAAATTAC 
ATTATCAAGA AAGCATGCAA CCAGAGGATT TCCTCAGGAG GTGTTGGTGC CCCCTCCCAA 
GGGGATTCCA TCATCGATGT GGTGAAGCGG TACGTCGGTA AAGGAGGGGG GGATTCCCTC 
ACCACTGGAC AGTATCCCTC TACAGGAGAA CTGGCAGATT CGCGTAGAGG TAACCCCAGC 
ATCAACAAAA TTAGCCTAAA ATATGCGAAC AAGAAGTTCA TCATTGAAAA CCTCCAAGTG 
GGGGATATCG TGGAAAGGCA CCTCGTGGAT GGAGATATCG TCTTGTTCAA TCGACAACCA 
TCTCTCCATC GAATGTCTAT CATGTGTCAC AAGGCGAGAG TGATGAAGTA CAAGACCTTT 
CGATTCAACG AATGTGTGTG CTCTCCGTAC AATGCAGATT TTGATGGAGA CGAAATGAAT 
TTACATGTGC CACAAACGGA GGAGGCAAGA GCAGAGGCTC TATACCTCAT GAATGTAAAG 
CACAATTTGA TAACTCCAAA AAATGGAGAA GTTATGATTG CCTTGACACA GGATTTCCTT 
TCAGCCTCCT ATGTCATCAC AAATAAGGAT ACCTTTCTCG ACAGAGACTC CTTCTGTTTA 
CTATGCTCCT ACTTCTCAGA TGCAAAGGTA GACATAGAAT TACCTGTGCC GGCTATATTA 
AAACCGAAGG AGCTTTGGAC GGGAAAGCAG TTGATAAGCG TCCTTATAAA GCCAAATAGG 
AAGGAAAACA CAATTATCAA TTTTGAAATG AAGGAGAGAG AATATTCAAC AAAAAATGGA 
GATCTCAAAT ATATGTGTCT GAATGATTCC TACGTATGTT TCTATAAATC TGAATTGATA 
TGTGGTTCGT TAGGAAAGAA GGTTTTGGGA TCATCCAAAT ATGGCTTATT CTACTACCTT 
ATCCACCACA ACTCTTCTCA CGTTGCGGTC AAAGTTATGA ACCGTTTTTC AAAACTGACC 
AGTAGATACT TCTCCAACAA AGGAATGACA ATAGGTATTG ACGATGTAAC TCCATCGGAG 
ACCCTAATTC AGAAGAAGAA GGATCTCCTC CAAGAAGGTT ATGAAAAGGT TAATAAGGAA 
ATAGTTCTTT ATAATGAGAA GAAAATGCAG ATACAACCGG GATGTACTTT GGAAGAAACG 
CTGGAAATTA AAGTAAAGAA TATTTTAGAT GATCTTCGAA ACGATGCAGG AAAGACGTGT 
AATCAGTATT TGGATCATTT GAACAAACCA CTTATTATGT TTAACTCTGG AGCCAAGGGG 
GCTCTCATCA ACATCGCTCA AATGATTGCT TGTGTTGGAC AGCAAAACGT TACTGGCCAG 
AGAATTAAAA ACGGATTCAT TAATAGAACC CTTCCTCATT TTAACTTCCA CTGTAAGGAC 
TCCGAAAGTA GGGGGTTCGT TCAGAACTCC TTCTACTCGG GTCTTAATCC CACCGAGTTC 
TTCTTTCATA CTATGTCTGG AAGAGAAGGG CTTGTGGATA CAGCTGTTAA GACCGCGGAA 
ACAGGCTACA TGCAAAGGAG ACTTATGAAA GCCCTTGAAG ACTTGTCCAT CCATTATGAT 
TACTCAGTTC GATCTTGCGA TAAACAAATT GTTCAATTTA TTTACGGGGA TGATGCACTG 
AACCCATCTT ATGTGGACAA CAATAATTCC TATGTACATC AGTTTGATAA AATCTTCGAT 
CACATTACAT CCATTTCTTC CAGCAACATT CTACTGTCAT ATGAGAAGCT CTCCCCTGCT 
TCGACAACCT GTTCGACAAC CTGTTCGACA ACCTGTTCGA CAAACGCTTC GACAAACGCT 
TCGACAAGCG GTTCCCCTAT TTCGTGCATC ATCAACCGAG TCGTTTCCAA AATGAGCGAC 
CCGGACTCGG TGCCCTTCAT CCCCTTATCG CACGATGAGC ATTTTGTGAG AAGGATCATG 
GAGAAACCCG AACTCACCCC CAGGTGGGGA AATAAAGTAG TAGCACAAGT AGTGGCGGAA 
GAAGAGGAAG CGACCAAAGG GGGAGATCCC CCTGATGTGA AAACTCCTCA TGATCCACCC 
GCCACGATGG AGGGCAAAGT CAAAGTGGAA ATAAGAAACG ACCAATATGT CGGCACGCAG 
GAACAGAAAC AATCTAAACG GCAGAAACGG TACAAAGGGA TGAAGCAGAC AAAGCATGGA 
CACCCAGAGG ATGAAGCCGG TGAACAGGCA ACTACCCAAA AGAACTGCGT CCTGCCGCCA 
CAGAAACATC AGTCTATTGC ACGATTCGTA AATGATTATC GAAACGTAGT TGAGATAAAG 
AAGCTGATGG ATAAGAATCA GGTCTGCCTG AGGGAGTCGG AAAAAAAACT TGTACGAAGT 
ATGTACAACC AGATGAGCAA AAACGTGGTG AAGAAAATCG AAATTATTAA CACGGTGTAT 
CGATCAGAGA GGGAAAAGGC GCTTAGTACT AAGGAACAGT GTCAAGGAGA GGCGAACGTG 
ATGGAAGTGA GTTATGGTGT AGATGCAAAC ACCAGTAGTG CAGATGCACA AGGTGGTTAT 
ATCCACACTA GGAGGAAGAA AGGGCACGTA CAGCCGCACA GCGGGACGAA TGGAAACCGT 
GAAAGGGATG AAATGCCCCG ATTATACGAA AATAAGCTGT ACAGGCAAAT GATAAAAAAC 
GTAATCGCAT TTGTGAGTGT ATTCGAATAC ATCGAAAAGA AGAAACAACA CTATATACTT 
CTGCCATATG AAGTTATAGA GTGGACGAAT TTTCTTCTGA ACTATCTACC AGAGTTAATG 
CCAACAAACA TATATGCTCA CACGGAACTA TCTCAAAGAG AAAGTCCAAC CCATCAGGAA 
AGAACCAAGA CGATGAATAT CTACATGAAG GAAATTAAAA AGTGGTTAAT AGTGAAGGCT 
ATCAATATAT ATAAGTTCTT TAAATACAAG AAGGGGGTTG AGTTAATGAG ACGGAGTGAT 
TACCTTGACT TCGTCTGCAC ACAAACGTAT GAACCATCTT ATCGATACCT CTTTTACGAT 
TATCCATTTG TTAACCTAAA GCAGTTGTAT CTGTTCATTT TTTTTAATGT CTCAAAATAC 
TTTAAGTATA TCTCCTCACC GGGGGATGCC GTGGGGTCCA TTTCGGCACA GTCCATTGGG 
GAACCCGGCA CGCAAATGAC ACTCAAGACT TTTCACTTTG CAGGCGTGGC CAGCATGAAC 
ATAACCCTGG GTGTACCACG AATCAAGGAA ATTATAAACG CGTCGAGCAA TATACAGACG 
CCTATACTAA ATATCCCCCT GCAGGTGAAG GACAACTACA ACTTTGCCCT AATGATGAAG 
TCAAAGTTGG AGAAAACCAC AATCAGGGAT ATCTGTCTGT ATATAAAAGA AGATTACACC 
AGTCGAGGCA TCTTCCTCTC CGTGAAATTC AACGAACAAC TCATCCACAA CCTATTCTTA 
AACATAAATG CCTATACCAT TAAGGATATA ATAATGAGGC AAAGTCACAT TAATAAAATT 
AAGATAAACA AAATTGTTGT TGAAGTGGTG AATGCGTACA AGTTACACAT GTCGTTGAAA 
AATGATGAGT TCTTATTTTT CCAAATCGAG TCTCTGAAGA AGGGACTCCT CGACCTCCTC 
ATTCATGGAG ACAAGGATAT AAAGCGTTGT ATCATTAAGA AGGAAGAAGT GGAGGTCACC 
GATGATGAGA TGGAGATAAC AGACGAAAGG GTGGGCGAAC TGTCTACTGG AATGCCTATC 
CATTCGGAAG ACCACAATCC CCCTTCGAAT CACTCCCCTA TTGCCAATGG GCACGACGCT 
AAACATAATA AAGGAGAAAA GGTAACCGAG AAGGAATGCT CTGGAAAGAA AACCCCCAAT 
GAGGAGAAAT TGCGGAACAT CATGGAAGCC TTTAAAGTAC AAATAAAGGA ACAGGTGTTA 
AAAACGGATA ACCGAGGAAG GGAAGAAGAA CCTATTGTCA ACTTAGATAC AATCAATATG 
GACACGTTAA ATTTAGAAAA AGTAGACCTC AACCAAATAA GGCATGAGAA GATTGACTTT 
TACGATAAGC ATGGTAATCT CTACACTGGA GGAAATGATC CGGAATGTAA AGAAAAAAAA 
AAAAAAAAAA AAAAAATTAT TTACTCGATC CTTGTAGAGG GTAACTCCCT CAATTACGTC 
CTGGGGTTGG AAGGAGTAGA CTTCAAGCAT ATAATTTCGA ACCATGTGAT AAATGTTTTT 
CAAGTCCTTG GAATAGAAGC TGCACGAGTA ACCATCATCA ATGAGATAAA GAAGTGTGTG 
GAGGCGTATA GCATAGACAT TGATATCAGG CACATCATGC TCCTGGCGGA TATCATGGCC 
TTCACGGGAG ATATCCTCGG GATAAACCGC TTCGGCATTC AGAAGGCCAG GCACAGCACA 
CTCATGTTGG CATCCTTCGA AGAGACCAAC GAACACCTCT TCGTTTCCTC TTTCTTTAAA 
AATAAGGATG AAATAAACAA CATCAGCGAG AGCATCATTG TTGGCAAAAA TATCCCCATT 
GGCACGGGAT CCTTCCAGCT TCTCTACGAC TACAAATTCA ACCGAGAGGA AAGGAAACTC 
ACTCTTCTGG AAAGGGCGCA ACAGGAAATG GCATCAGGGA GAGATGTCGT GAAACTGGCA 
CTACACAATG GCAAGGGAAG TCAGGGCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002260047.1
CDS	1..5913
Translation	MDELRVKSNDVEELRRIIQRTSLRKGFVQDVKSNWEIKSLQFGIMNKEEIIKCSEVKILNREMYKNNTGIPYPYGVLDLKLGAHKSNSLCETCNKNFMNCSGHFGYIELNYPLFHVGYYKYIIYILYCICKVCSHILLTNEKLEFYTNLKRRASEDKFFKKHIFKKILNSCKKVTKCYRCGYPQGVIKKIIKPSLDQFMKLKHVIKIKENGKMVIKEDDLNSLYVLNLFKNMNPYHVKLLNVENPEKLLITALLVPPNTIRPSVIIDEHGTAEDDLTCILSEITQLNNTINNQCSNGYQTNQFLGNVEFLQLQITRFINSDSPAVSQLLATQNISKPGRGICQRLKGKEGRFRCNLSGKRVDFSSRTVISPDPNISIDEVVIPEIIAMRLTYPEKVNRYNIERLKKFIKNGTNTWPGANYIIKKACNQRISSGGVGAPSQGDSIIDVVKRYVGKGGGDSLTTGQYPSTGELADSRRGNPSINKISLKYANKKFIIENLQVGDIVERHLVDGDIVLFNRQPSLHRMSIMCHKARVMKYKTFRFNECVCSPYNADFDGDEMNLHVPQTEEARAEALYLMNVKHNLITPKNGEVMIALTQDFLSASYVITNKDTFLDRDSFCLLCSYFSDAKVDIELPVPAILKPKELWTGKQLISVLIKPNRKENTIINFEMKEREYSTKNGDLKYMCLNDSYVCFYKSELICGSLGKKVLGSSKYGLFYYLIHHNSSHVAVKVMNRFSKLTSRYFSNKGMTIGIDDVTPSETLIQKKKDLLQEGYEKVNKEIVLYNEKKMQIQPGCTLEETLEIKVKNILDDLRNDAGKTCNQYLDHLNKPLIMFNSGAKGALINIAQMIACVGQQNVTGQRIKNGFINRTLPHFNFHCKDSESRGFVQNSFYSGLNPTEFFFHTMSGREGLVDTAVKTAETGYMQRRLMKALEDLSIHYDYSVRSCDKQIVQFIYGDDALNPSYVDNNNSYVHQFDKIFDHITSISSSNILLSYEKLSPASTTCSTTCSTTCSTNASTNASTSGSPISCIINRVVSKMSDPDSVPFIPLSHDEHFVRRIMEKPELTPRWGNKVVAQVVAEEEEATKGGDPPDVKTPHDPPATMEGKVKVEIRNDQYVGTQEQKQSKRQKRYKGMKQTKHGHPEDEAGEQATTQKNCVLPPQKHQSIARFVNDYRNVVEIKKLMDKNQVCLRESEKKLVRSMYNQMSKNVVKKIEIINTVYRSEREKALSTKEQCQGEANVMEVSYGVDANTSSADAQGGYIHTRRKKGHVQPHSGTNGNRERDEMPRLYENKLYRQMIKNVIAFVSVFEYIEKKKQHYILLPYEVIEWTNFLLNYLPELMPTNIYAHTELSQRESPTHQERTKTMNIYMKEIKKWLIVKAINIYKFFKYKKGVELMRRSDYLDFVCTQTYEPSYRYLFYDYPFVNLKQLYLFIFFNVSKYFKYISSPGDAVGSISAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIINASSNIQTPILNIPLQVKDNYNFALMMKSKLEKTTIRDICLYIKEDYTSRGIFLSVKFNEQLIHNLFLNINAYTIKDIIMRQSHINKIKINKIVVEVVNAYKLHMSLKNDEFLFFQIESLKKGLLDLLIHGDKDIKRCIIKKEEVEVTDDEMEITDERVGELSTGMPIHSEDHNPPSNHSPIANGHDAKHNKGEKVTEKECSGKKTPNEEKLRNIMEAFKVQIKEQVLKTDNRGREEEPIVNLDTINMDTLNLEKVDLNQIRHEKIDFYDKHGNLYTGGNDPECKEKKKKKKKIIYSILVEGNSLNYVLGLEGVDFKHIISNHVINVFQVLGIEAARVTIINEIKKCVEAYSIDIDIRHIMLLADIMAFTGDILGINRFGIQKARHSTLMLASFEETNEHLFVSSFFKNKDEINNISESIIVGKNIPIGTGSFQLLYDYKFNREERKLTLLERAQQEMASGRDVVKLALHNGKGSQGN

Target ORF information:

RefSeq Version	XM_002260011.1
Organism	Plasmodium knowlesi strain H
Definition	Plasmodium knowlesi strain H DNA-directed RNA polymerase 3 largest subunit,putative partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002260011.1

ORF Insert Sequence:

ATGGATGAAT TGCGAGTAAA GTCCAACGAT GTGGAGGAGT TGCGGAGGAT AATTCAAAGG 
ACCAGCCTGC GGAAGGGATT CGTACAAGAT GTGAAGAGCA ACTGGGAAAT CAAAAGTCTC 
CAGTTCGGAA TTATGAACAA GGAAGAAATA ATAAAATGCT CAGAAGTGAA AATTTTAAAC 
AGAGAAATGT ACAAAAACAA CACAGGTATT CCCTACCCTT ATGGAGTCTT GGATCTCAAG 
TTAGGAGCTC ACAAAAGTAA CTCTCTGTGT GAAACATGTA ACAAAAATTT TATGAACTGT 
TCAGGTCATT TTGGATACAT TGAATTAAAC TACCCACTCT TTCATGTGGG GTACTACAAG 
TACATCATCT ATATCCTGTA CTGCATTTGC AAAGTCTGCT CACATATTTT ACTAACCAAT 
GAAAAGCTTG AATTTTATAC GAATCTGAAA AGACGTGCAT CGGAGGATAA GTTTTTCAAA 
AAACACATTT TTAAGAAAAT CTTAAACAGT TGTAAAAAGG TGACAAAATG CTACCGTTGT 
GGATATCCTC AAGGAGTGAT AAAAAAAATT ATAAAACCCA GTTTAGATCA ATTCATGAAA 
TTAAAACATG TAATTAAAAT CAAAGAAAAT GGTAAAATGG TAATAAAAGA GGATGATTTA 
AATAGTCTTT ACGTTTTAAA TCTCTTCAAA AATATGAATC CTTACCATGT GAAACTTTTA 
AATGTGGAAA ATCCAGAGAA GCTACTCATA ACGGCTTTGT TGGTACCTCC CAACACCATT 
AGACCATCAG TTATAATTGA TGAACATGGT ACAGCAGAGG ATGACTTGAC TTGTATTCTA 
TCAGAAATCA CACAACTTAA CAACACCATT AATAATCAGT GCAGTAATGG TTACCAGACT 
AATCAGTTCT TAGGTAATGT AGAATTTCTT CAACTTCAGA TTACAAGGTT CATCAATAGC 
GATTCCCCTG CTGTGTCTCA GTTATTAGCT ACTCAAAATA TTTCCAAACC AGGTAGAGGA 
ATCTGTCAGA GGCTGAAAGG AAAAGAAGGT AGATTCAGAT GTAACTTATC CGGTAAAAGG 
GTAGACTTTT CAAGCAGAAC TGTCATTTCA CCGGATCCTA ATATCTCCAT TGATGAGGTT 
GTTATTCCGG AAATTATCGC TATGAGGCTT ACGTACCCGG AGAAGGTTAA TCGGTACAAC 
ATTGAGAGGT TAAAGAAATT TATAAAGAAC GGAACAAACA CATGGCCTGG AGCAAATTAC 
ATTATCAAGA AAGCATGCAA CCAGAGGATT TCCTCAGGAG GTGTTGGTGC CCCCTCCCAA 
GGGGATTCCA TCATCGATGT GGTGAAGCGG TACGTCGGTA AAGGAGGGGG GGATTCCCTC 
ACCACTGGAC AGTATCCCTC TACAGGAGAA CTGGCAGATT CGCGTAGAGG TAACCCCAGC 
ATCAACAAAA TTAGCCTAAA ATATGCGAAC AAGAAGTTCA TCATTGAAAA CCTCCAAGTG 
GGGGATATCG TGGAAAGGCA CCTCGTGGAT GGAGATATCG TCTTGTTCAA TCGACAACCA 
TCTCTCCATC GAATGTCTAT CATGTGTCAC AAGGCGAGAG TGATGAAGTA CAAGACCTTT 
CGATTCAACG AATGTGTGTG CTCTCCGTAC AATGCAGATT TTGATGGAGA CGAAATGAAT 
TTACATGTGC CACAAACGGA GGAGGCAAGA GCAGAGGCTC TATACCTCAT GAATGTAAAG 
CACAATTTGA TAACTCCAAA AAATGGAGAA GTTATGATTG CCTTGACACA GGATTTCCTT 
TCAGCCTCCT ATGTCATCAC AAATAAGGAT ACCTTTCTCG ACAGAGACTC CTTCTGTTTA 
CTATGCTCCT ACTTCTCAGA TGCAAAGGTA GACATAGAAT TACCTGTGCC GGCTATATTA 
AAACCGAAGG AGCTTTGGAC GGGAAAGCAG TTGATAAGCG TCCTTATAAA GCCAAATAGG 
AAGGAAAACA CAATTATCAA TTTTGAAATG AAGGAGAGAG AATATTCAAC AAAAAATGGA 
GATCTCAAAT ATATGTGTCT GAATGATTCC TACGTATGTT TCTATAAATC TGAATTGATA 
TGTGGTTCGT TAGGAAAGAA GGTTTTGGGA TCATCCAAAT ATGGCTTATT CTACTACCTT 
ATCCACCACA ACTCTTCTCA CGTTGCGGTC AAAGTTATGA ACCGTTTTTC AAAACTGACC 
AGTAGATACT TCTCCAACAA AGGAATGACA ATAGGTATTG ACGATGTAAC TCCATCGGAG 
ACCCTAATTC AGAAGAAGAA GGATCTCCTC CAAGAAGGTT ATGAAAAGGT TAATAAGGAA 
ATAGTTCTTT ATAATGAGAA GAAAATGCAG ATACAACCGG GATGTACTTT GGAAGAAACG 
CTGGAAATTA AAGTAAAGAA TATTTTAGAT GATCTTCGAA ACGATGCAGG AAAGACGTGT 
AATCAGTATT TGGATCATTT GAACAAACCA CTTATTATGT TTAACTCTGG AGCCAAGGGG 
GCTCTCATCA ACATCGCTCA AATGATTGCT TGTGTTGGAC AGCAAAACGT TACTGGCCAG 
AGAATTAAAA ACGGATTCAT TAATAGAACC CTTCCTCATT TTAACTTCCA CTGTAAGGAC 
TCCGAAAGTA GGGGGTTCGT TCAGAACTCC TTCTACTCGG GTCTTAATCC CACCGAGTTC 
TTCTTTCATA CTATGTCTGG AAGAGAAGGG CTTGTGGATA CAGCTGTTAA GACCGCGGAA 
ACAGGCTACA TGCAAAGGAG ACTTATGAAA GCCCTTGAAG ACTTGTCCAT CCATTATGAT 
TACTCAGTTC GATCTTGCGA TAAACAAATT GTTCAATTTA TTTACGGGGA TGATGCACTG 
AACCCATCTT ATGTGGACAA CAATAATTCC TATGTACATC AGTTTGATAA AATCTTCGAT 
CACATTACAT CCATTTCTTC CAGCAACATT CTACTGTCAT ATGAGAAGCT CTCCCCTGCT 
TCGACAACCT GTTCGACAAC CTGTTCGACA ACCTGTTCGA CAAACGCTTC GACAAACGCT 
TCGACAAGCG GTTCCCCTAT TTCGTGCATC ATCAACCGAG TCGTTTCCAA AATGAGCGAC 
CCGGACTCGG TGCCCTTCAT CCCCTTATCG CACGATGAGC ATTTTGTGAG AAGGATCATG 
GAGAAACCCG AACTCACCCC CAGGTGGGGA AATAAAGTAG TAGCACAAGT AGTGGCGGAA 
GAAGAGGAAG CGACCAAAGG GGGAGATCCC CCTGATGTGA AAACTCCTCA TGATCCACCC 
GCCACGATGG AGGGCAAAGT CAAAGTGGAA ATAAGAAACG ACCAATATGT CGGCACGCAG 
GAACAGAAAC AATCTAAACG GCAGAAACGG TACAAAGGGA TGAAGCAGAC AAAGCATGGA 
CACCCAGAGG ATGAAGCCGG TGAACAGGCA ACTACCCAAA AGAACTGCGT CCTGCCGCCA 
CAGAAACATC AGTCTATTGC ACGATTCGTA AATGATTATC GAAACGTAGT TGAGATAAAG 
AAGCTGATGG ATAAGAATCA GGTCTGCCTG AGGGAGTCGG AAAAAAAACT TGTACGAAGT 
ATGTACAACC AGATGAGCAA AAACGTGGTG AAGAAAATCG AAATTATTAA CACGGTGTAT 
CGATCAGAGA GGGAAAAGGC GCTTAGTACT AAGGAACAGT GTCAAGGAGA GGCGAACGTG 
ATGGAAGTGA GTTATGGTGT AGATGCAAAC ACCAGTAGTG CAGATGCACA AGGTGGTTAT 
ATCCACACTA GGAGGAAGAA AGGGCACGTA CAGCCGCACA GCGGGACGAA TGGAAACCGT 
GAAAGGGATG AAATGCCCCG ATTATACGAA AATAAGCTGT ACAGGCAAAT GATAAAAAAC 
GTAATCGCAT TTGTGAGTGT ATTCGAATAC ATCGAAAAGA AGAAACAACA CTATATACTT 
CTGCCATATG AAGTTATAGA GTGGACGAAT TTTCTTCTGA ACTATCTACC AGAGTTAATG 
CCAACAAACA TATATGCTCA CACGGAACTA TCTCAAAGAG AAAGTCCAAC CCATCAGGAA 
AGAACCAAGA CGATGAATAT CTACATGAAG GAAATTAAAA AGTGGTTAAT AGTGAAGGCT 
ATCAATATAT ATAAGTTCTT TAAATACAAG AAGGGGGTTG AGTTAATGAG ACGGAGTGAT 
TACCTTGACT TCGTCTGCAC ACAAACGTAT GAACCATCTT ATCGATACCT CTTTTACGAT 
TATCCATTTG TTAACCTAAA GCAGTTGTAT CTGTTCATTT TTTTTAATGT CTCAAAATAC 
TTTAAGTATA TCTCCTCACC GGGGGATGCC GTGGGGTCCA TTTCGGCACA GTCCATTGGG 
GAACCCGGCA CGCAAATGAC ACTCAAGACT TTTCACTTTG CAGGCGTGGC CAGCATGAAC 
ATAACCCTGG GTGTACCACG AATCAAGGAA ATTATAAACG CGTCGAGCAA TATACAGACG 
CCTATACTAA ATATCCCCCT GCAGGTGAAG GACAACTACA ACTTTGCCCT AATGATGAAG 
TCAAAGTTGG AGAAAACCAC AATCAGGGAT ATCTGTCTGT ATATAAAAGA AGATTACACC 
AGTCGAGGCA TCTTCCTCTC CGTGAAATTC AACGAACAAC TCATCCACAA CCTATTCTTA 
AACATAAATG CCTATACCAT TAAGGATATA ATAATGAGGC AAAGTCACAT TAATAAAATT 
AAGATAAACA AAATTGTTGT TGAAGTGGTG AATGCGTACA AGTTACACAT GTCGTTGAAA 
AATGATGAGT TCTTATTTTT CCAAATCGAG TCTCTGAAGA AGGGACTCCT CGACCTCCTC 
ATTCATGGAG ACAAGGATAT AAAGCGTTGT ATCATTAAGA AGGAAGAAGT GGAGGTCACC 
GATGATGAGA TGGAGATAAC AGACGAAAGG GTGGGCGAAC TGTCTACTGG AATGCCTATC 
CATTCGGAAG ACCACAATCC CCCTTCGAAT CACTCCCCTA TTGCCAATGG GCACGACGCT 
AAACATAATA AAGGAGAAAA GGTAACCGAG AAGGAATGCT CTGGAAAGAA AACCCCCAAT 
GAGGAGAAAT TGCGGAACAT CATGGAAGCC TTTAAAGTAC AAATAAAGGA ACAGGTGTTA 
AAAACGGATA ACCGAGGAAG GGAAGAAGAA CCTATTGTCA ACTTAGATAC AATCAATATG 
GACACGTTAA ATTTAGAAAA AGTAGACCTC AACCAAATAA GGCATGAGAA GATTGACTTT 
TACGATAAGC ATGGTAATCT CTACACTGGA GGAAATGATC CGGAATGTAA AGAAAAAAAA 
AAAAAAAAAA AAAAAATTAT TTACTCGATC CTTGTAGAGG GTAACTCCCT CAATTACGTC 
CTGGGGTTGG AAGGAGTAGA CTTCAAGCAT ATAATTTCGA ACCATGTGAT AAATGTTTTT 
CAAGTCCTTG GAATAGAAGC TGCACGAGTA ACCATCATCA ATGAGATAAA GAAGTGTGTG 
GAGGCGTATA GCATAGACAT TGATATCAGG CACATCATGC TCCTGGCGGA TATCATGGCC 
TTCACGGGAG ATATCCTCGG GATAAACCGC TTCGGCATTC AGAAGGCCAG GCACAGCACA 
CTCATGTTGG CATCCTTCGA AGAGACCAAC GAACACCTCT TCGTTTCCTC TTTCTTTAAA 
AATAAGGATG AAATAAACAA CATCAGCGAG AGCATCATTG TTGGCAAAAA TATCCCCATT 
GGCACGGGAT CCTTCCAGCT TCTCTACGAC TACAAATTCA ACCGAGAGGA AAGGAAACTC 
ACTCTTCTGG AAAGGGCGCA ACAGGAAATG GCATCAGGGA GAGATGTCGT GAAACTGGCA 
CTACACAATG GCAAGGGAAG TCAGGGCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the simian and human malaria parasite Plasmodium knowlesi.
Nature455(7214)799-803(2008 Oct)
Pain A,Böhme U,Berry AE,Mungall K,Finn RD,Jackson AP,Mourier T,Mistry J,Pasini EM,Aslett MA,Balasubrammaniam S,Borgwardt K,Brooks K,Carret C,Carver TJ,Cherevach I,Chillingworth T,Clark TG,Galinski MR,Hall N,Harper D,Harris D,Hauser H,Ivens A,Janssen CS,Keane T,Larke N,Lapp S,Marti M,Moule S,Meyer IM,Ormond D,Peters N,Sanders M,Sanders S,Sargeant TJ,Simmonds M,Smith F,Squares R,Thurston S,Tivey AR,Walker D,White B,Zuiderwijk E,Churcher C,Quail MA,Cowman AF,Turner CM,Rajandream MA,Kocken CH,Thomas AW,Newbold CI,Barrell BG,Berriman M

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PKH_122370 cDNA ORF clone, Plasmodium knowlesi strain H

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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