PKH_120470 cDNA ORF clone, Plasmodium knowlesi strain H

Gene
Clones
gRNAs

Summary

Gene Symbol	PKH_120470
Entrez Gene ID	7322191
Full Name	glutamate--tRNA ligase, putative
Gene Type	protein-coding
Organism	Plasmodium knowlesi strain H

mRNA and Protein(s)

mRNA	Protein	Name
XM_002259821.1	XP_002259857.1	glutamate--tRNA ligase, putative

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the simian and human malaria parasite Plasmodium knowlesi.
Pain A, Böhme U, Berry AE, Mungall K, Finn RD, Jackson AP, Mourier T, Mistry J, Pasini EM, Aslett MA, Balasubrammaniam S, Borgwardt K, Brooks K, Carret C, Carver TJ, Cherevach I, Chillingworth T, Clark TG, Galinski MR, Hall N, Harper D, Harris D, Hauser H, Ivens A, Janssen CS, Keane T, Larke N, Lapp S, Marti M, Moule S, Meyer IM, Ormond D, Peters N, Sanders M, Sanders S, Sargeant TJ, Simmonds M, Smith F, Squares R, Thurston S, Tivey AR, Walker D, White B, Zuiderwijk E, Churcher C, Quail MA, Cowman AF, Turner CM, Rajandream MA, Kocken CH, Thomas AW, Newbold CI, Barrell BG, Berriman M
Nature455(7214)799-803(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PKH_120470 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKH_120470 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPs02827	XM_002259821.1 Latest version!	Plasmodium knowlesi strain H glutamate--tRNA ligase, putative partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PKH_120470 ( XM_002259821.1 ) cDNA ORF clone, Plasmodium knowlesi strain H -> XP_002259857.1 Plasmodium knowlesi strain H glutamate--tRNA ligase, putative partial mRNA.

Price & Availability↑

CloneID	OPs02827
Clone ID Related Accession (Same CDS sequence)	XM_002259821.1
Accession Version	XM_002259821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Plasmodium knowlesi strain H
Product	glutamate--tRNA ligase, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_011913). COMPLETENESS: incomplete on both ends.

ATGATAGGAG GTGACAAAAT TATCATCTAC TACGGAAAGA AATACCCCTT CGTATGTAGA 
ACAATACTTA ACGTACATAA GATTCTCAGT CAAAAGGAGA ACGTAGAGGG AAGAAGTAAC 
CACTTTCCGA ATGACAAAGT AAAATTTGTC AAGGACGAAA ATGTAGAAGA AATAAAGGTA 
GAGTTTGTCA ACAAAGCAGA AGAAGAAAAC GGAGATAAGT GCATAAATAC CACGGACAAA 
ATATTTGCAA AGACTTTAGA CGAAATATTG CAAACAAAGT TATACCATTC ATTCTGTGAG 
AATAACAAAA AAGAAGACAA ACAGACAGAC GTCTATGTAC AAGCACAGTA CGACGAATGG 
GTAGACTATT TCAGGAACAA ACATATGCAA AAGGAAATAC TAATAATTTG TGAGTACGTA 
AATAAACACC TCCATTTAAA TACCTTTATA TGCGGCGAGT ATTTAACCCT ATCAGATTTA 
TATGTGTATT ACGAAATGTA CAGATATTTT AATGTGGCCA ATTGTTACAA TGTGAAGTAC 
ACAAAACAGT TTAGGAATAT CAATAGGTGG TTCAAGTTAA TCAACTGTTT AGTGAATTAT 
CCAGATGAAG AGTTAAGCAC AATGGTAAAG AAGGAACTAG AAAAGAACCA AGTAAATATT 
ACAAAAGATG CAAATAGCAA ACAATTGTTA AAACAGAAAA CTGTGTGCAC TTCCTATGGT 
GGTAAATTAG AAAATGCAGA AAAGGGAAAT GTAATTACGA GGTTTCCTCC AGAACCATCG 
GGATACTTAC ATATTGGCCA TGCCAAAGCA GCTTTCCTCA ACAGTTATTA TGCAAATATG 
TATGAGGGAA AAATGCTACT CCGTTTTGAT GATACAAACC CAGTTTTGGA AGACATAAAG 
TTTGAGACAT CCATCATGGA AGATCTAGAA AATTTAGGAT TAAAATATGA AAAGATTAGC 
TACACATCAG ACTATTTTGC AGATCTAGAG GATTACTGCA TTAAGTTAAT AAAAATGGGA 
AAGGCATATG CAGATGATAC GAATGTAGAA GAAATGAGGA ATCAGAGAGG GGAAGGAATT 
GAATCCCGAA ATAGAGAGAA CAGCGTAGAA CAGAATCTAC AATTGTTTGA AGAAATGCGT 
AAGGGAACCG AAATTGGACA GAAGAATTGT ATTCGTGCAA AAATTGATAT GCAGAGTAAG 
AACAAATGCA TGCGAGATCC CGTGTTATAC AGATGCATAG TAGATACACC TCATCACAGA 
CACGGGTTCA AGTTCAAGTG CTACCCCACG TACGATTTTG CATGCCCTAT AATTGATTCC 
ATTGAAGGAG TAACGCACGC ATTGAGAACG AACGAATACA GTGACCGCAT AGAACAATAC 
AACTGGATTA TTACAACCTT CCAGTTGAGA AAAGTATACA TTTACGAATT TAGTAGAATC 
TCTTTTGTCA GAACGGTTAT GTCAAAGAGG AAGTTAAAAT GGTTTGTTGA AAATAAGGTT 
GTGGATGGAT GGCTAGATCC ACGTATGCCC ACTATCAAAG GGATCCTCAG AAGAGGATTA 
ACGAAAGAAG CCTTGTTCCA ATTTATACTA GAACAAGGAC CCTCCAAATC AGGAAATCTC 
ATGCAATGGG ATAAACTATG GTCCATCAAC AAACAAATAA TCGACCCAAT CATCCCCAGG 
TTTGCAGCAG TAGATAAAAA AAAAGGAGTT ATTCTTAAAT TAACAGACTT GACCGAGGAC 
ATTGTCGAAA AGACGAGAGA TCTACACATG AAAAATAAAT CCCTTGGCAC ATGCTCCATG 
CTTTATACCA ACCGTTTCTT TATCGAATTG GAGGATGCAC AAACGTTAGG AGAGCAAGAA 
GAAATTACCC TCATCAAACT AGGAAATGTT ATCATCACCA GTATTGTAAA AGGGGACGAC 
AATGATAATG GAACAGATAC CCCAGTCATT AAGGAAGTCA TCGGAAAATC CAATTTCGAT 
GGAGACTTCA AAACGACAAA AAAAAAAATA CACTGGGTGC CATACATACC CGAAAAATTA 
ATCACCTGTA CGCTTTACGA ATATGACCAC TTAATTACCG TTGACAAATT TGAAAATGAT 
AATAAGGAAG ACTGGACTAA ATTTATCAAC TCCAATAGCA AGTACGAAAC GGTGGTGTAC 
TCCGAACCAG CCATCACCTC CCTTAAAGTG TCAGACAGAT TTCAGTTCGA AAGAAGGGGG 
TACTTCATTG TCGACAAGGT TGTGGACAAC CACCTTCATT TGATTAAAAT CCCCGACGGA 
AAATCCAAAA ACATGTCTAT TATCAGTTCC AAGGTAAACC CCCAAAATTT GGCCGGCACC 
AAAACTAAGG TCCCAGACGC TCTGGAGAAG TCTGCCAAGG GGGACCAAGC TGTCGAAGCG 
AAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002259857.1
CDS	1..2409
Translation	MIGGDKIIIYYGKKYPFVCRTILNVHKILSQKENVEGRSNHFPNDKVKFVKDENVEEIKVEFVNKAEEENGDKCINTTDKIFAKTLDEILQTKLYHSFCENNKKEDKQTDVYVQAQYDEWVDYFRNKHMQKEILIICEYVNKHLHLNTFICGEYLTLSDLYVYYEMYRYFNVANCYNVKYTKQFRNINRWFKLINCLVNYPDEELSTMVKKELEKNQVNITKDANSKQLLKQKTVCTSYGGKLENAEKGNVITRFPPEPSGYLHIGHAKAAFLNSYYANMYEGKMLLRFDDTNPVLEDIKFETSIMEDLENLGLKYEKISYTSDYFADLEDYCIKLIKMGKAYADDTNVEEMRNQRGEGIESRNRENSVEQNLQLFEEMRKGTEIGQKNCIRAKIDMQSKNKCMRDPVLYRCIVDTPHHRHGFKFKCYPTYDFACPIIDSIEGVTHALRTNEYSDRIEQYNWIITTFQLRKVYIYEFSRISFVRTVMSKRKLKWFVENKVVDGWLDPRMPTIKGILRRGLTKEALFQFILEQGPSKSGNLMQWDKLWSINKQIIDPIIPRFAAVDKKKGVILKLTDLTEDIVEKTRDLHMKNKSLGTCSMLYTNRFFIELEDAQTLGEQEEITLIKLGNVIITSIVKGDDNDNGTDTPVIKEVIGKSNFDGDFKTTKKKIHWVPYIPEKLITCTLYEYDHLITVDKFENDNKEDWTKFINSNSKYETVVYSEPAITSLKVSDRFQFERRGYFIVDKVVDNHLHLIKIPDGKSKNMSIISSKVNPQNLAGTKTKVPDALEKSAKGDQAVEAKK

Target ORF information:

RefSeq Version	XM_002259821.1
Organism	Plasmodium knowlesi strain H
Definition	Plasmodium knowlesi strain H glutamate--tRNA ligase, putative partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002259821.1

ORF Insert Sequence:

ATGATAGGAG GTGACAAAAT TATCATCTAC TACGGAAAGA AATACCCCTT CGTATGTAGA 
ACAATACTTA ACGTACATAA GATTCTCAGT CAAAAGGAGA ACGTAGAGGG AAGAAGTAAC 
CACTTTCCGA ATGACAAAGT AAAATTTGTC AAGGACGAAA ATGTAGAAGA AATAAAGGTA 
GAGTTTGTCA ACAAAGCAGA AGAAGAAAAC GGAGATAAGT GCATAAATAC CACGGACAAA 
ATATTTGCAA AGACTTTAGA CGAAATATTG CAAACAAAGT TATACCATTC ATTCTGTGAG 
AATAACAAAA AAGAAGACAA ACAGACAGAC GTCTATGTAC AAGCACAGTA CGACGAATGG 
GTAGACTATT TCAGGAACAA ACATATGCAA AAGGAAATAC TAATAATTTG TGAGTACGTA 
AATAAACACC TCCATTTAAA TACCTTTATA TGCGGCGAGT ATTTAACCCT ATCAGATTTA 
TATGTGTATT ACGAAATGTA CAGATATTTT AATGTGGCCA ATTGTTACAA TGTGAAGTAC 
ACAAAACAGT TTAGGAATAT CAATAGGTGG TTCAAGTTAA TCAACTGTTT AGTGAATTAT 
CCAGATGAAG AGTTAAGCAC AATGGTAAAG AAGGAACTAG AAAAGAACCA AGTAAATATT 
ACAAAAGATG CAAATAGCAA ACAATTGTTA AAACAGAAAA CTGTGTGCAC TTCCTATGGT 
GGTAAATTAG AAAATGCAGA AAAGGGAAAT GTAATTACGA GGTTTCCTCC AGAACCATCG 
GGATACTTAC ATATTGGCCA TGCCAAAGCA GCTTTCCTCA ACAGTTATTA TGCAAATATG 
TATGAGGGAA AAATGCTACT CCGTTTTGAT GATACAAACC CAGTTTTGGA AGACATAAAG 
TTTGAGACAT CCATCATGGA AGATCTAGAA AATTTAGGAT TAAAATATGA AAAGATTAGC 
TACACATCAG ACTATTTTGC AGATCTAGAG GATTACTGCA TTAAGTTAAT AAAAATGGGA 
AAGGCATATG CAGATGATAC GAATGTAGAA GAAATGAGGA ATCAGAGAGG GGAAGGAATT 
GAATCCCGAA ATAGAGAGAA CAGCGTAGAA CAGAATCTAC AATTGTTTGA AGAAATGCGT 
AAGGGAACCG AAATTGGACA GAAGAATTGT ATTCGTGCAA AAATTGATAT GCAGAGTAAG 
AACAAATGCA TGCGAGATCC CGTGTTATAC AGATGCATAG TAGATACACC TCATCACAGA 
CACGGGTTCA AGTTCAAGTG CTACCCCACG TACGATTTTG CATGCCCTAT AATTGATTCC 
ATTGAAGGAG TAACGCACGC ATTGAGAACG AACGAATACA GTGACCGCAT AGAACAATAC 
AACTGGATTA TTACAACCTT CCAGTTGAGA AAAGTATACA TTTACGAATT TAGTAGAATC 
TCTTTTGTCA GAACGGTTAT GTCAAAGAGG AAGTTAAAAT GGTTTGTTGA AAATAAGGTT 
GTGGATGGAT GGCTAGATCC ACGTATGCCC ACTATCAAAG GGATCCTCAG AAGAGGATTA 
ACGAAAGAAG CCTTGTTCCA ATTTATACTA GAACAAGGAC CCTCCAAATC AGGAAATCTC 
ATGCAATGGG ATAAACTATG GTCCATCAAC AAACAAATAA TCGACCCAAT CATCCCCAGG 
TTTGCAGCAG TAGATAAAAA AAAAGGAGTT ATTCTTAAAT TAACAGACTT GACCGAGGAC 
ATTGTCGAAA AGACGAGAGA TCTACACATG AAAAATAAAT CCCTTGGCAC ATGCTCCATG 
CTTTATACCA ACCGTTTCTT TATCGAATTG GAGGATGCAC AAACGTTAGG AGAGCAAGAA 
GAAATTACCC TCATCAAACT AGGAAATGTT ATCATCACCA GTATTGTAAA AGGGGACGAC 
AATGATAATG GAACAGATAC CCCAGTCATT AAGGAAGTCA TCGGAAAATC CAATTTCGAT 
GGAGACTTCA AAACGACAAA AAAAAAAATA CACTGGGTGC CATACATACC CGAAAAATTA 
ATCACCTGTA CGCTTTACGA ATATGACCAC TTAATTACCG TTGACAAATT TGAAAATGAT 
AATAAGGAAG ACTGGACTAA ATTTATCAAC TCCAATAGCA AGTACGAAAC GGTGGTGTAC 
TCCGAACCAG CCATCACCTC CCTTAAAGTG TCAGACAGAT TTCAGTTCGA AAGAAGGGGG 
TACTTCATTG TCGACAAGGT TGTGGACAAC CACCTTCATT TGATTAAAAT CCCCGACGGA 
AAATCCAAAA ACATGTCTAT TATCAGTTCC AAGGTAAACC CCCAAAATTT GGCCGGCACC 
AAAACTAAGG TCCCAGACGC TCTGGAGAAG TCTGCCAAGG GGGACCAAGC TGTCGAAGCG 
AAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the simian and human malaria parasite Plasmodium knowlesi.
Nature455(7214)799-803(2008 Oct)
Pain A,Böhme U,Berry AE,Mungall K,Finn RD,Jackson AP,Mourier T,Mistry J,Pasini EM,Aslett MA,Balasubrammaniam S,Borgwardt K,Brooks K,Carret C,Carver TJ,Cherevach I,Chillingworth T,Clark TG,Galinski MR,Hall N,Harper D,Harris D,Hauser H,Ivens A,Janssen CS,Keane T,Larke N,Lapp S,Marti M,Moule S,Meyer IM,Ormond D,Peters N,Sanders M,Sanders S,Sargeant TJ,Simmonds M,Smith F,Squares R,Thurston S,Tivey AR,Walker D,White B,Zuiderwijk E,Churcher C,Quail MA,Cowman AF,Turner CM,Rajandream MA,Kocken CH,Thomas AW,Newbold CI,Barrell BG,Berriman M

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PKH_120470 cDNA ORF clone, Plasmodium knowlesi strain H

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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