GAREM1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAREM1
Entrez Gene ID	468614
Full Name	GRB2 associated regulator of MAPK1 subtype 1
Synonyms	FAM59A,GAREM
General protein information	Preferred Names GRB2 associated regulator of MAPK1 subtype 1 Names GRB2-associated and regulator of MAPK protein 1 GRB2 associated, regulator of MAPK1 family with sequence similarity 59, member A
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016933497.1	XP_016788986.1	GRB2-associated and regulator of MAPK protein 1 isoform X2
XM_016933498.1	XP_016788987.1	GRB2-associated and regulator of MAPK protein 1 isoform X3
XM_009433843.2	XP_009432118.2	GRB2-associated and regulator of MAPK protein 1 isoform X1
XM_016933498.2	XP_016788987.1	GRB2-associated and regulator of MAPK protein 1 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Garem	NP_001028617.2
Xenopus tropicalis (tropical clawed frog)	garem	XP_004915258.1
Homo sapiens (human)	GAREM	NP_001229338.1
Pan troglodytes (chimpanzee)	FAM59A	XP_524002.3
Canis lupus familiaris (dog)	FAM59A	XP_005623042.1
Rattus norvegicus (Norway rat)	Garem	XP_006254547.1
Gallus gallus (chicken)	FAM59A	XP_419182.2
Danio rerio (zebrafish)	garem	NP_956463.1
Bos taurus (cattle)	GAREM	XP_005224117.1

Gene Ontology

The following GAREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc73878	XM_009433843.2 Latest version!	Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPc73879	XM_016933497.1 Latest version!	Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPc73880	XM_016933498.2 Latest version!	Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OPc73880	XM_016933498.1	Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAREM1 ( XM_009433843.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009432118.2 Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc73878
Clone ID Related Accession (Same CDS sequence)	XM_009433843.2
Accession Version	XM_009433843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036897.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009433843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG GCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTACCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTTATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGGG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACTGACTGG AATGAGGACT GCAAGAGCCC CAAGAAGGGC 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTTTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAAGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGTG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAA TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC 
ACTAAAACTG ACACAAGTCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA 
GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG 
TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT 
GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA 
CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCTT GGCCAGCCCC 
ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC 
TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG 
TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA 
ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA 
GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC 
TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG TGACGGTTCC 
CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA 
CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG 
AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAACTG 
CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009432118.2
CDS	91..2721
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDNGECVEGLRENDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAMKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNISVTKTDTSPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	XM_009433843.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009433843.2

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG GCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTACCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTTATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGGG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACTGACTGG AATGAGGACT GCAAGAGCCC CAAGAAGGGC 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTTTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAAGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGTG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAA TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCAGCGTC 
ACTAAAACTG ACACAAGTCC TTCTGAAAGC ACTCCTGTTT CCTGCTATCC ATGTAACCGA 
GTGAAAACTG ATTCTGTGGA CCTGAAATCC CCGTTTGGAA GTCCTTCTGC TGAAGCTGTG 
TCCTCTCGGC TCTCATGGCC TAACCATTAT TCAGGAGCAT CAGAAAGCCA GACCAGGAGT 
GACTTCCTGC TGGATCCAAG CAGGAGTTAT AGTTACCCTA GACAAAAGAC GCCAGGCACA 
CCAAAGAGAA ACTGCCCAGC ACCTTTTGAT TTTGATGGCT GTGAGCTCTT GGCCAGCCCC 
ACTAGCCCAG TCACTGCAGA ATTCAGTAGC AGCGTCTCTG GTTGTCCCAA GTCAGCCAGC 
TACTCTCTGG AGAGCACAGA TGTGAAATCT CTTGCAGCTG GTGTGACAAA GCAGAGTACG 
TCATGCCCTG CCTTACCCCC CAGGGCTCCA AAACTAGTGG AAGAGAAGGT CGCCTCCGAA 
ACATCTCCTT TGCCTCTGAA AATTGATGGT GCTGAGGAAG ACCCCAAGTC TGGGTCACCA 
GATCTCTCGG AGGACCAGTA TTTTGTTAAA AAGGGCATGC AGGACATCTT CTCTGCCTCC 
TACCCTTTCT CATCTCCGCT CCATCTCCAG CTGGCCCCCA GATCCTGTGG TGACGGTTCC 
CCATGGCAGC CACCTGCTGA CCTATCAGGA CTCTCTATAG AGGAAGTGTC CAAGTCACTA 
CGGTTCATTG GTTTGTCCGA AGATGTCATA TCATTCTTTG TTACTGAAAA GATTGATGGG 
AACCTGCTTG TTCAGCTAAC GGAAGAAATC CTCTCAGAGG ATTTCAAATT GAGCAAACTG 
CAGGTGAAGA AGATAATGCA ATTCATTAAT GGCTGGAGGC CCAAAATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_016933497.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016788986.1 Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc73879
Clone ID Related Accession (Same CDS sequence)	XM_016933497.1
Accession Version	XM_016933497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036897.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG GCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTACCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTTATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGGG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACTGACTGG AATGAGGACT GCAAGAGCCC CAAGAAGGGC 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTTTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAAGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGTG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAA TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT 
AAAACTGACA CAAGTCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG 
AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC 
TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC 
TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA 
AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCTTGGC CAGCCCCACT 
AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC 
TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA 
TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA 
TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT 
CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC 
CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGTGA CGGTTCCCCA 
TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG 
TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC 
CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAACTGCAG 
GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016788986.1
CDS	91..2718
Translation	MDPAPSLGCSLKDVKWSSVAVPLDLLVSTYRLPQIARLDNGECVEGLRENDYLLIHSCRQWTTITAHSLEEGHYVIGPKIEIPVHYAGQFKLLEQDRDIKEPVQYFNSVEEVAKAFPERVYVMEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAMKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNIVTKTDTSPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	XM_016933497.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016933497.1

ORF Insert Sequence:

ATGGACCCGG CGCCCTCGCT GGGCTGCAGC CTCAAGGATG TGAAGTGGAG CTCGGTGGCC 
GTGCCGCTCG ACCTCCTGGT CAGCACTTAC CGGCTGCCCC AGATCGCGCG GCTGGACAAC 
GGAGAGTGCG TAGAAGGGCT GCGGGAAAAT GACTACCTGC TGATTCATTC CTGCCGCCAG 
TGGACCACCA TCACTGCCCA CAGCTTGGAG GAGGGTCACT ATGTCATTGG GCCAAAGATA 
GAGATTCCGG TACATTATGC AGGGCAATTC AAGCTGCTGG AACAAGACCG AGATATAAAG 
GAGCCAGTGC AATATTTCAA CAGTGTGGAG GAGGTGGCTA AGGCATTTCC TGAACGCGTG 
TACGTCATGG AGGATATCAC ATTCAACGTG AAGGTTGCTT CAGGTGAATG CAATGAAGAC 
ACTGAAGTTT ACAACATCAC CCTGTGTACT GGGGATGAAC TCACTCTAAT GGGGCAGGCA 
GAAATCCTTT ATGCAAAGAC ATTCAAGGAA AAGTCACGAC TCAACACAAT CTTCAAAAAG 
ATTGGGAAGC TCAATTCCAT CAGCAAGCTG GGAAAAGGCA AAATGCCGTG CCTTATTTGT 
ATGAATCACC GGACCAACGA AAGCATTAGC CTTCCATTCC AGTGCAAGGG CAGATTTAGC 
ACCCGAAGTC CCCTGGAACT TCAGATGCAA GAGGGCGAAC ACACCATCCG CAACATTGTG 
GAGAAAACCA GGCTTCCTGT GAATGTGACT GTGCCAAGCC CTCCACCGAG AAACCCATAC 
GACCTCCACT TCATCCGTGA GGGGCACCGC TATAAGTTTG TGAACATCCA GACCAAGACG 
GTGGTGGTTT GCTGTGTGCT GCGGAACAAC AAGATCCTCC CCATGCACTT TCCTTTGCAC 
TTGACTGTCC CCAAGTTCAG CCTCCCAGAA CACCTGGTGA AGGGAGAGGG CTGGCCCGAA 
ACCCTGGTCC ATCACTGGCT AGGTATCTGC CAAGAACAGT TCGACATCGA TGAGTATTCA 
CGGGCTGTCC GTGATGTGAA AACTGACTGG AATGAGGACT GCAAGAGCCC CAAGAAGGGC 
CGGTGCTCTG GCCACAACCA CGTGCCCAAT TCGCTCAGCT ACGCCCGCGA TGAGCTCACC 
CAGTCCTTCC ACCGACTTTC GGTCTGTGTG TATGGCAACA ATCTCCATGG CAACAGTGAG 
GTGAACCTTC ATGGTTGCAG GGACCTGGGG GGAGATTGGG CTCCCTTTCC TCATGACATC 
CTGCCCTATC AGGACTCTGG AGATAGTGGG AGCGACTACC TTTTCCCAGA AGCTAGTGAA 
GAATCAGCAG GCATCCCGGG AAAGTCAGAA CTTCCCTACG AAGAGCTGTG GCTGGAAGAA 
GGCAAGCCCA GCCATCAGCC TCTCACTCGC TCTCTGAGTG AGAAGAACAG ATGTGATCAG 
TTTAGAGGTT CTGTCCGATC CAAATGTGCG ACTTCTCCTC TTCCCATCCC TGGGACTCTG 
GGAGCAGCAA TGAAGTCTTC AGATACTGCC CTACCTCCAC CTCCAGTGCC TCCCAAATCT 
GAAGCCGTCA GAGAAGAATG CCGGCTCCTG AACGCCCCAC CTGTTCCACC CCGAAGCGCA 
AAGCCTTTGT CCACCAGTCC CTCCATCCCT CCTCGCACAG TCAAGCCAGC GCGGCAACAG 
ACTCGCTCTC CCAGCCCCAC CTTGTCCTAC TATTCTTCAG GGCTACACAA CATCGTCACT 
AAAACTGACA CAAGTCCTTC TGAAAGCACT CCTGTTTCCT GCTATCCATG TAACCGAGTG 
AAAACTGATT CTGTGGACCT GAAATCCCCG TTTGGAAGTC CTTCTGCTGA AGCTGTGTCC 
TCTCGGCTCT CATGGCCTAA CCATTATTCA GGAGCATCAG AAAGCCAGAC CAGGAGTGAC 
TTCCTGCTGG ATCCAAGCAG GAGTTATAGT TACCCTAGAC AAAAGACGCC AGGCACACCA 
AAGAGAAACT GCCCAGCACC TTTTGATTTT GATGGCTGTG AGCTCTTGGC CAGCCCCACT 
AGCCCAGTCA CTGCAGAATT CAGTAGCAGC GTCTCTGGTT GTCCCAAGTC AGCCAGCTAC 
TCTCTGGAGA GCACAGATGT GAAATCTCTT GCAGCTGGTG TGACAAAGCA GAGTACGTCA 
TGCCCTGCCT TACCCCCCAG GGCTCCAAAA CTAGTGGAAG AGAAGGTCGC CTCCGAAACA 
TCTCCTTTGC CTCTGAAAAT TGATGGTGCT GAGGAAGACC CCAAGTCTGG GTCACCAGAT 
CTCTCGGAGG ACCAGTATTT TGTTAAAAAG GGCATGCAGG ACATCTTCTC TGCCTCCTAC 
CCTTTCTCAT CTCCGCTCCA TCTCCAGCTG GCCCCCAGAT CCTGTGGTGA CGGTTCCCCA 
TGGCAGCCAC CTGCTGACCT ATCAGGACTC TCTATAGAGG AAGTGTCCAA GTCACTACGG 
TTCATTGGTT TGTCCGAAGA TGTCATATCA TTCTTTGTTA CTGAAAAGAT TGATGGGAAC 
CTGCTTGTTC AGCTAACGGA AGAAATCCTC TCAGAGGATT TCAAATTGAG CAAACTGCAG 
GTGAAGAAGA TAATGCAATT CATTAATGGC TGGAGGCCCA AAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_016933498.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016788987.1 Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

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CloneID	OPc73880
Clone ID Related Accession (Same CDS sequence)	XM_016933498.1 , XM_016933498.2
Accession Version	XM_016933498.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006485.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTTAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGGGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACTGA CTGGAATGAG GACTGCAAGA GCCCCAAGAA GGGCCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TTTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA AGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGTGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAATGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA 
ACTGACACAA GTCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA 
ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT 
CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC 
CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG 
AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCTTGGCCAG CCCCACTAGC 
CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT 
CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC 
CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT 
CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC 
TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT 
TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGTGACGG TTCCCCATGG 
CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC 
ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG 
CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ACTGCAGGTG 
AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016788987.1
CDS	290..2554
Translation	MEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAMKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNISVTKTDTSPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	XM_016933498.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016933498.1

ORF Insert Sequence:

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTTAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGGGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACTGA CTGGAATGAG GACTGCAAGA GCCCCAAGAA GGGCCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TTTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA AGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGTGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAATGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA 
ACTGACACAA GTCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA 
ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT 
CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC 
CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG 
AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCTTGGCCAG CCCCACTAGC 
CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT 
CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC 
CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT 
CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC 
TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT 
TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGTGACGG TTCCCCATGG 
CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC 
ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG 
CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ACTGCAGGTG 
AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAREM1 ( XM_016933498.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016788987.1 Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

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CloneID	OPc73880
Clone ID Related Accession (Same CDS sequence)	XM_016933498.1 , XM_016933498.2
Accession Version	XM_016933498.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	GRB2-associated and regulator of MAPK protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036897.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016933498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTTAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGGGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACTGA CTGGAATGAG GACTGCAAGA GCCCCAAGAA GGGCCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TTTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA AGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGTGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAATGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA 
ACTGACACAA GTCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA 
ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT 
CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC 
CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG 
AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCTTGGCCAG CCCCACTAGC 
CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT 
CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC 
CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT 
CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC 
TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT 
TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGTGACGG TTCCCCATGG 
CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC 
ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG 
CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ACTGCAGGTG 
AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016788987.1
CDS	288..2552
Translation	MEDITFNVKVASGECNEDTEVYNITLCTGDELTLMGQAEILYAKTFKEKSRLNTIFKKIGKLNSISKLGKGKMPCLICMNHRTNESISLPFQCKGRFSTRSPLELQMQEGEHTIRNIVEKTRLPVNVTVPSPPPRNPYDLHFIREGHRYKFVNIQTKTVVVCCVLRNNKILPMHFPLHLTVPKFSLPEHLVKGEGWPETLVHHWLGICQEQFDIDEYSRAVRDVKTDWNEDCKSPKKGRCSGHNHVPNSLSYARDELTQSFHRLSVCVYGNNLHGNSEVNLHGCRDLGGDWAPFPHDILPYQDSGDSGSDYLFPEASEESAGIPGKSELPYEELWLEEGKPSHQPLTRSLSEKNRCDQFRGSVRSKCATSPLPIPGTLGAAMKSSDTALPPPPVPPKSEAVREECRLLNAPPVPPRSAKPLSTSPSIPPRTVKPARQQTRSPSPTLSYYSSGLHNISVTKTDTSPSESTPVSCYPCNRVKTDSVDLKSPFGSPSAEAVSSRLSWPNHYSGASESQTRSDFLLDPSRSYSYPRQKTPGTPKRNCPAPFDFDGCELLASPTSPVTAEFSSSVSGCPKSASYSLESTDVKSLAAGVTKQSTSCPALPPRAPKLVEEKVASETSPLPLKIDGAEEDPKSGSPDLSEDQYFVKKGMQDIFSASYPFSSPLHLQLAPRSCGDGSPWQPPADLSGLSIEEVSKSLRFIGLSEDVISFFVTEKIDGNLLVQLTEEILSEDFKLSKLQVKKIMQFINGWRPKI

Target ORF information:

RefSeq Version	XM_016933498.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GRB2 associated regulator of MAPK1 subtype 1 (GAREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016933498.2

ORF Insert Sequence:

ATGGAGGATA TCACATTCAA CGTGAAGGTT GCTTCAGGTG AATGCAATGA AGACACTGAA 
GTTTACAACA TCACCCTGTG TACTGGGGAT GAACTCACTC TAATGGGGCA GGCAGAAATC 
CTTTATGCAA AGACATTCAA GGAAAAGTCA CGACTCAACA CAATCTTCAA AAAGATTGGG 
AAGCTCAATT CCATCAGCAA GCTGGGAAAA GGCAAAATGC CGTGCCTTAT TTGTATGAAT 
CACCGGACCA ACGAAAGCAT TAGCCTTCCA TTCCAGTGCA AGGGCAGATT TAGCACCCGA 
AGTCCCCTGG AACTTCAGAT GCAAGAGGGC GAACACACCA TCCGCAACAT TGTGGAGAAA 
ACCAGGCTTC CTGTGAATGT GACTGTGCCA AGCCCTCCAC CGAGAAACCC ATACGACCTC 
CACTTCATCC GTGAGGGGCA CCGCTATAAG TTTGTGAACA TCCAGACCAA GACGGTGGTG 
GTTTGCTGTG TGCTGCGGAA CAACAAGATC CTCCCCATGC ACTTTCCTTT GCACTTGACT 
GTCCCCAAGT TCAGCCTCCC AGAACACCTG GTGAAGGGAG AGGGCTGGCC CGAAACCCTG 
GTCCATCACT GGCTAGGTAT CTGCCAAGAA CAGTTCGACA TCGATGAGTA TTCACGGGCT 
GTCCGTGATG TGAAAACTGA CTGGAATGAG GACTGCAAGA GCCCCAAGAA GGGCCGGTGC 
TCTGGCCACA ACCACGTGCC CAATTCGCTC AGCTACGCCC GCGATGAGCT CACCCAGTCC 
TTCCACCGAC TTTCGGTCTG TGTGTATGGC AACAATCTCC ATGGCAACAG TGAGGTGAAC 
CTTCATGGTT GCAGGGACCT GGGGGGAGAT TGGGCTCCCT TTCCTCATGA CATCCTGCCC 
TATCAGGACT CTGGAGATAG TGGGAGCGAC TACCTTTTCC CAGAAGCTAG TGAAGAATCA 
GCAGGCATCC CGGGAAAGTC AGAACTTCCC TACGAAGAGC TGTGGCTGGA AGAAGGCAAG 
CCCAGCCATC AGCCTCTCAC TCGCTCTCTG AGTGAGAAGA ACAGATGTGA TCAGTTTAGA 
GGTTCTGTCC GATCCAAATG TGCGACTTCT CCTCTTCCCA TCCCTGGGAC TCTGGGAGCA 
GCAATGAAGT CTTCAGATAC TGCCCTACCT CCACCTCCAG TGCCTCCCAA ATCTGAAGCC 
GTCAGAGAAG AATGCCGGCT CCTGAACGCC CCACCTGTTC CACCCCGAAG CGCAAAGCCT 
TTGTCCACCA GTCCCTCCAT CCCTCCTCGC ACAGTCAAGC CAGCGCGGCA ACAGACTCGC 
TCTCCCAGCC CCACCTTGTC CTACTATTCT TCAGGGCTAC ACAACATCAG CGTCACTAAA 
ACTGACACAA GTCCTTCTGA AAGCACTCCT GTTTCCTGCT ATCCATGTAA CCGAGTGAAA 
ACTGATTCTG TGGACCTGAA ATCCCCGTTT GGAAGTCCTT CTGCTGAAGC TGTGTCCTCT 
CGGCTCTCAT GGCCTAACCA TTATTCAGGA GCATCAGAAA GCCAGACCAG GAGTGACTTC 
CTGCTGGATC CAAGCAGGAG TTATAGTTAC CCTAGACAAA AGACGCCAGG CACACCAAAG 
AGAAACTGCC CAGCACCTTT TGATTTTGAT GGCTGTGAGC TCTTGGCCAG CCCCACTAGC 
CCAGTCACTG CAGAATTCAG TAGCAGCGTC TCTGGTTGTC CCAAGTCAGC CAGCTACTCT 
CTGGAGAGCA CAGATGTGAA ATCTCTTGCA GCTGGTGTGA CAAAGCAGAG TACGTCATGC 
CCTGCCTTAC CCCCCAGGGC TCCAAAACTA GTGGAAGAGA AGGTCGCCTC CGAAACATCT 
CCTTTGCCTC TGAAAATTGA TGGTGCTGAG GAAGACCCCA AGTCTGGGTC ACCAGATCTC 
TCGGAGGACC AGTATTTTGT TAAAAAGGGC ATGCAGGACA TCTTCTCTGC CTCCTACCCT 
TTCTCATCTC CGCTCCATCT CCAGCTGGCC CCCAGATCCT GTGGTGACGG TTCCCCATGG 
CAGCCACCTG CTGACCTATC AGGACTCTCT ATAGAGGAAG TGTCCAAGTC ACTACGGTTC 
ATTGGTTTGT CCGAAGATGT CATATCATTC TTTGTTACTG AAAAGATTGA TGGGAACCTG 
CTTGTTCAGC TAACGGAAGA AATCCTCTCA GAGGATTTCA AATTGAGCAA ACTGCAGGTG 
AAGAAGATAA TGCAATTCAT TAATGGCTGG AGGCCCAAAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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GAREM1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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