EDN1 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDN1
Entrez Gene ID	100009270
Full Name	endothelin 1
Synonyms	ET-1
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101696.1	NP_001095166.1	endothelin-1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Nuclear factor-κB is involved in oxyhemoglobin-induced endothelin-1 expression in cerebrovascular muscle cells of the rabbit basilar artery.
Cheng G, Yu WH, Yan C, Liu Y, Li WJ, Zhang DD, Liu N
Neuroreport27(12)875-82(2016 08)

Mechanisms Underlying Endothelin-1 Level Elevations Caused by Excessive Fluoride Exposure.
Sun L, Gao Y, Zhang W, Liu X, Li B, Cui X, Sun D
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(5)861-873(2016)

Variation of plasma levels of endothelin, calcitonin gene-related peptide, nitric oxide, and malondialdehyde in acute myocardial ischemia reperfusion injury in a rabbit model.
Zhao YB, Wang YZ, Yue YH, Zhao WC, Feng GX
Genetics and molecular research : GMR14(2)5577-84(2015 May)

Assessment of circulatory and pulmonary endothelin-1 levels in a lavage-induced surfactant-depleted lung injury rabbit model with repeated open endotracheal suctioning and hyperinflation.
Kamiyama J, Jesmin S, Sakuramoto H, Shimojo N, Islam MM, Khatun T, Oki M, Kawano S, Mizutani T
Life sciences118(2)370-8(2014 Nov)

Effects of endothelin-1 chronic stimulation on electrical restitution, beat-to-beat variability of repolarization, and ventricular arrhythmogenesis.
Liu T, Qin M, Shi SB, Chen Z, Wang T, Huang CX
Journal of cardiovascular pharmacology62(6)549-58(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EDN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb30123	NM_001101696.1 Latest version!	Oryctolagus cuniculus endothelin 1 (EDN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDN1 ( NM_001101696.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001095166.1 Oryctolagus cuniculus endothelin 1 (EDN1), mRNA.

Price & Availability↑

CloneID	OOb30123
Clone ID Related Accession (Same CDS sequence)	NM_001101696.1
Accession Version	NM_001101696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562256000000
Organism	Oryctolagus cuniculus(rabbit)
Product	endothelin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X59931.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: X59931.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1876818, SAMEA1876819 [ECO:0000348] ##Evidence-Data-END##

ATGGATTACT TCTCCATGAT GGTCTCTCTG CTGTTGGTGG CTTTCCATGG AGCTCCCGAG 
ACAGCGGCCT CGGGCACGGA GCTCAGCACA GGAGCGGAAA ACCCAGGGGA GAAGCCCCCG 
GCCAGCGCAC CTTGGCGGCC GCGCCGGTCC AAGCGGTGCT CCTGCTCCTC GCTGATGGAC 
AAGGAGTGTG TCTACTTCTG CCACCTGGAC ATCATCTGGG TCAACACCCC CGGGCACATC 
GTCCCGTATG GACTTGGAAG CCCGTCCAGG TCCAAGCGAT CCTTGAAGGA TTTATTTCCT 
ACCAGGGCAG CGTACCACAA GAACAGGTGC CAGTGCACGA GCCCACACGA CAAGAAGTGC 
TGGAATTTTT GCCAAGCAGG AACGGAACTC AGAGCCCAAG AGACTATGGA GAAAGGCAGG 
AACAACCTTA AGAAAGGCAA AGACTGCTCC AAGCTTGGAA AGAAGTGTAT TCTTCAAAAG 
CTGATGCAAG GGAGAAAAAT AAGAAGGTTG GAGGCCATCA GCAACAGCAT CAAGACATCT 
TTTCATGCCG CCCAGCTGAG AGCCCAGCTC CACCGAGAGC AGAAAGTGAC CCACAACCGG 
ACACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095166.1
CDS	114..722
Translation	MDYFSMMVSLLLVAFHGAPETAASGTELSTGAENPGEKPPASAPWRPRRSKRCSCSSLMDKECVYFCHLDIIWVNTPGHIVPYGLGSPSRSKRSLKDLFPTRAAYHKNRCQCTSPHDKKCWNFCQAGTELRAQETMEKGRNNLKKGKDCSKLGKKCILQKLMQGRKIRRLEAISNSIKTSFHAAQLRAQLHREQKVTHNRTH

Target ORF information:

RefSeq Version	NM_001101696.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus endothelin 1 (EDN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101696.1

ORF Insert Sequence:

ATGGATTACT TCTCCATGAT GGTCTCTCTG CTGTTGGTGG CTTTCCATGG AGCTCCCGAG 
ACAGCGGCCT CGGGCACGGA GCTCAGCACA GGAGCGGAAA ACCCAGGGGA GAAGCCCCCG 
GCCAGCGCAC CTTGGCGGCC GCGCCGGTCC AAGCGGTGCT CCTGCTCCTC GCTGATGGAC 
AAGGAGTGTG TCTACTTCTG CCACCTGGAC ATCATCTGGG TCAACACCCC CGGGCACATC 
GTCCCGTATG GACTTGGAAG CCCGTCCAGG TCCAAGCGAT CCTTGAAGGA TTTATTTCCT 
ACCAGGGCAG CGTACCACAA GAACAGGTGC CAGTGCACGA GCCCACACGA CAAGAAGTGC 
TGGAATTTTT GCCAAGCAGG AACGGAACTC AGAGCCCAAG AGACTATGGA GAAAGGCAGG 
AACAACCTTA AGAAAGGCAA AGACTGCTCC AAGCTTGGAA AGAAGTGTAT TCTTCAAAAG 
CTGATGCAAG GGAGAAAAAT AAGAAGGTTG GAGGCCATCA GCAACAGCAT CAAGACATCT 
TTTCATGCCG CCCAGCTGAG AGCCCAGCTC CACCGAGAGC AGAAAGTGAC CCACAACCGG 
ACACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear factor-κB is involved in oxyhemoglobin-induced endothelin-1 expression in cerebrovascular muscle cells of the rabbit basilar artery.
Neuroreport27(12)875-82(2016 08)
Cheng G,Yu WH,Yan C,Liu Y,Li WJ,Zhang DD,Liu N

Mechanisms Underlying Endothelin-1 Level Elevations Caused by Excessive Fluoride Exposure.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(5)861-873(2016)
Sun L,Gao Y,Zhang W,Liu X,Li B,Cui X,Sun D

Variation of plasma levels of endothelin, calcitonin gene-related peptide, nitric oxide, and malondialdehyde in acute myocardial ischemia reperfusion injury in a rabbit model.
Genetics and molecular research : GMR14(2)5577-84(2015 May)
Zhao YB,Wang YZ,Yue YH,Zhao WC,Feng GX

Assessment of circulatory and pulmonary endothelin-1 levels in a lavage-induced surfactant-depleted lung injury rabbit model with repeated open endotracheal suctioning and hyperinflation.
Life sciences118(2)370-8(2014 Nov)
Kamiyama J,Jesmin S,Sakuramoto H,Shimojo N,Islam MM,Khatun T,Oki M,Kawano S,Mizutani T

Effects of endothelin-1 chronic stimulation on electrical restitution, beat-to-beat variability of repolarization, and ventricular arrhythmogenesis.
Journal of cardiovascular pharmacology62(6)549-58(2013 Dec)
Liu T,Qin M,Shi SB,Chen Z,Wang T,Huang CX