LOC100116996 cDNA ORF clone, Nasonia vitripennis(jewel wasp)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100116996
Entrez Gene ID	100116996
Full Name	spectrin alpha chain
Synonyms	alpha-Spec
Gene Type	protein-coding
Organism	Nasonia vitripennis(jewel wasp)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016981977.1	XP_016837466.1	spectrin alpha chain isoform X1
XM_001601302.4	XP_001601352.1	spectrin alpha chain isoform X2
XM_016981977.2	XP_016837466.1	spectrin alpha chain isoform X1
XM_031922932.1	XP_031778792.1	spectrin alpha chain isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100116996 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100116996 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONd04093	XM_001601302.4 Latest version!	Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ONd04093	XM_031922932.1 Latest version!	Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ONd22430	XM_016981977.2 Latest version!	Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ONd22430	XM_016981977.1	Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100116996 ( XM_001601302.4 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_001601352.1 Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X2, mRNA.

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CloneID	ONd04093
Clone ID Related Accession (Same CDS sequence)	XM_001601302.4 , XM_031922932.1
Accession Version	XM_001601302.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464105600000
Organism	Nasonia vitripennis(jewel wasp)
Product	spectrin alpha chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015868.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2016 this sequence version replaced XM_001601302.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGC AGATCACCCC CAAGGAGGTG AAAATCCTCG AGAACCCAGA GGACATCCAG 
GAGAGGAGAG AGCAGGTTCT GGGACGTTAT GCCAATTTTA AGTCGGAGGC GCGCAGCAAG 
CGCGACAAAC TCGAGGACTC TCGTCGTTTC CAGTACTTTA AACGAGATGC AGATGAACTC 
GAGGGTTGGA TTTATGAGAA GTTGCAAGCT GCTTCCGATG AAAGCTACAA AGATCCGACC 
AACCTACAGG CAAAGATTCA AAAGCACCAG GCTTTTGAAG CCGAAGTCGC TGCTCATTCG 
AATGCGATTG TTCTATTAGA CAATACTGGA TCGGAAATGA TTGCTCAGCA CCACTTTGCT 
AGTGACATCA TTCGTAAAAG GCTTGAGGAA TTGCATCGCT TATGGGAATT GCTTCTTAAT 
CGACTGGCTG ACAAGGGATT GAAGCTGCAG CAGGCTTTGG TTCTTGTACA ATTCATTCGC 
CATTGCGATG AAGTCATGTT TTGGATACAT GACAAGGAAG TCTTCGTCAC CACTGATGAG 
TTTGGACAAG ATTTGGAGCA CGTAGAAGTA CTCCAAAGGA AATTTGACGA ATTCCAGAAA 
GACATGACCA GCCAAGAATA TCGTGTAACT GAAGTTAACG AATTAGCCGA TAAATTACTT 
TTGGACGCTC ATCCTGAGCG CGATACTATT TTAAGACGCA AAGAAGAATT GAATGAATCA 
TGGCAGCGAT TGAAACAACT CGCTATTCTG CGTCAAGAAA AATTGTTCGG TGCTCACGAA 
ATTCAGAGAT TCAATCGTGA TGCCGACGAG ACAATGGCTT GGATTGGAGA GAAAGACGCA 
GTACTGTCTT CCGATGATTT TGGTCGCGAT TTGGCTAGTG TACAGCTGCT CCAGCGTACT 
CACGAGAACA TTGAACGCGA TTTGGCTGCG CTCGAAGACA AGGTGGCTAC GCTTAGCACT 
GAAGCTGACA GACTAGCCGG CATCCATCAA GCCGAGCACT CTCAGCAAAT TCAGTCCAAG 
AGAGCAGAGA TTCTACAGTC CTGGGAATCG CTTACTGCTA AGGCAAAAGA ACGCCGTTTC 
AAGCTCGACG AGTCTTACTA CTTACATCGC TTCCTTGCTG ACTACCGCGA CCTTGTTTCA 
TGGATGAACA ACATGCGAGC AATCATCTCA GCTGATGAGC TTGCAAAGGA CGTGGCTGGA 
GCTGAGGCGC TTCTTGAACG TCACCAAGAG CACAAGGGCG AGATCGATGC TCGTGCCGAC 
AGCTTTGATT CAACCGGCCT CGCCGGAAGT AAGCTTCTCG AACGTCAACA CTACGCTGCG 
TCGGAGGTAG CCATCAAGCT CGAATCTCTC GCTGCTGACA AGGCTTCGTT GCTTGAACTT 
TGGGAAAAGC GACGCATTCT TTACGAGCAG TGCATGGATC TGCAGCTCTT TTACCGTGAC 
ACTGAACAAG CTGATGCCTG GATGGCCAAG CAGGAAGCTT TCCTCGCCAA CGAGGATCTT 
GGAGATTCCC TTGACAGCGT CGAGGCTCTC ATCAAGAAGC ACGAGGACTT TGACAAGTCC 
CTAGCTGCCC AGGAGGAAAA GATCAAGGCG CTTGACGAGT TTGCCAGCAA GCTCATCGAA 
GGCGAACATT ATGCGGCCGA TGAAGTAGCC CAGCGCCGGC AGGCTCTTTT GCAACGTCGC 
TCAGCCCTCC TTGAAAAGTC GGCTTTGCGC CGTCGTGCCC TTGAGGACGC CTACAAGCTG 
CAGCAGTTCG AGCGTGACTG CGATGAGACC AAAGGCTGGG TCAACGAGAA GCTTAAGTTC 
GCCACCGATG ACAGCTACCT CGACCCTACC AATCTCAATG GTAAGCTGCA GAAACACCAG 
AATTTCGAGC AGGAGCTTCA AGCAAACAAA ACGCGCATGC AAGAAATGGT CGCTCAGGGC 
CAGGAACTCA TCGAAGCCGA GCATTACGCT CGCGAGCGCA TCCAGTCGCG CATCAAGGAG 
ATCGTCGAGC TCTGGGAAAG TCTGACCGAA GCCACTGAGA AGAAGGGCAA CAAGCTTCAG 
GAAGCTGCGC AACAACAGCA ATTCAACCGC ACTGTCGAGG ACGTCGAACT CTGGCTCTCA 
GAGGTCGAAG GTCAGCTCTT GTCCGAAGAC TATGGCAAGG ACCTTACCAG CGTGCAGAAC 
CTTCAGAAAA AGCACGCCCT GCTCGAGGCC GACGTCGCCT CCCATCAAGA GCGTATCGCC 
TCTATTGCCC AGGCAGCCGA GCAGTTCGTC GCTGGAGGTC ACTTTGACGC CGACAACATC 
CGTCAGAAGC AGCAACAACT GCAGGGACGC TATTCTGCTT TGGAGCAGCC TATGCAGATG 
CGCAAGCAGC GCTTGCTGGA CTCGCTGCAG GTACAGCAGC TGTTCCGCGA TGTCGAAGAC 
GAGGAGGCCT GGATCCGCGA GAAGGAGCCT GTGGCAGCCA GCACCAACCG TGGTCGCGAT 
CTCATTGGTG TGCAGAACCT GCAAAAGAAG CACCAGGCCG TCCTTGCTGA AATCAACAAT 
CATGAGCCCC GAGTTGCAGC CGTCTGCGCA GCTGGCTCAC GCATGTTGAG CGATGGTCAT 
TTCGCAGCTG AAGAGATCAG CCAGAGACTG GCTAGTCTCG ATGAACATTG GGGACAGTTG 
AAGGAGAAGG CTCGCCAGCG TAAGAACGAC CTTGATGACT CGCTCCAGGC TCACCAATAC 
TTCGCCGATG CCAATGAAGC CGAGTCTTGG ATGAAGGAGA AGCGCCCCAT CGTCATGAAC 
GCCGATTACG GCAAAGACGA GGATAGTAGC GAGGCTCTGC TCAAGAAACA CGAGGCTCTC 
GTGAGCGATC TCGAAGCTTT TGCTAGCACC ATTGCAGCGC TCAAGGAACA GGCAGCCGCC 
TGCCGTCAGC AAGAGACACC TGCTGTCGAC GTAGCTGGAA AGGAATGCGT TGTCGCACTC 
TACGATTACA CCGAGAAGTC TCCTCGCGAG GTCTCTATGA AGAAAGGAGA CACCCTTACA 
CTCCTCAACA GCAGCAACAA GGACTGGTGG AAGGTGGAGG TAAACGATCG TCAGGGCTTC 
GTGCCAGCCG CCTATGTCAA GCGCGTAGAA CCGGACGCGG GTCTTAGTGC TTCTCAGCAG 
AATCTGGCTC GCGAGCAGGG CTCCATTGCC GCTCGCCAGG CCCAGATTGA AGCCCAATAC 
GAAGACTTGC TGCGATTGGC CAGAGAAAGA CAAAACAAGC TTAATGAAAC CGCCAAGGCG 
TACGTGTTGG TTCGCGAGGC CGCTGAACTG GCCACCTGGA TCAAGGGCAA GGAGAACCAT 
GCTCAGGTAC AGGATGTAGG CGAGGATTTG GAACAAGTCG AGGTTATGCA GAAGAAGTTC 
GACGATTTCC GAGCTGATCT GAAGGCAAAC GAGGTGCGTC TCGCAGAGAT GAACGAGATC 
GCCGTGCAGC TGATGAGCGT CGGCCAGACC GAGGCAGCTC TTAAGATCCA GACGCAGATC 
CAAGAGCTTA ACGAAAAATG GACGAGTCTG CAGACTCTAA CAGCCGAGCG CGCCAGTCAG 
CTGGACTCTG CTCACGAAGT ACAGCGCTTC CATCGCGATG TCGACGACAC CAAAGACTGG 
ATTCGCGAGA AGGATGCTGC CCTGAACAAC GATGACCTGG GCAAGGACTT GCGCAGCGTG 
CAAACTCTGC TCCGCAAGCA CGAAGGTCTG GAGCGCGACC TCGCTGCCCT TGGGGACAAG 
ATTAAGCAGT TGGACGAAAC AGCAAACCGC TTGATGCAGT CGCATCCTGA TACGGCCGAG 
CAAACCTACA CCAAGCAGAA GGAGATCAAC GAGGAATGGA CTCAGCTTAC TGCCAAAGCT 
GATTCTAGAA AGAAGAAGCT GCTCGATTCT TATGACCTGC AACGCTATCT CAGCGACTAT 
CGGGATCTTA TGGCCTGGAT TAATTCGATG ATGGGTCTGG TAGCTTCCGA AGAATTGGCT 
AACGACGTCA CCGGTGCTGA GGCTCTGCTT GAACGTCACC AGGAACATCG CATGGAAATC 
GACGCCCGCG TTGGAACTTT CCAAGCTTTC GAGCTCTTCG GCCAGCAACT CTTGCAATCC 
AACCACTATG CTAGCGTTGA GATACAAGAG AAACTCGAAT CTATGAACGA GGCTCGTCAA 
GAACTTGAGA AGGCTTGGAT ACAGCGCCGT ATGCAGTTGG ACCAGAACCT CGAGCTGCAA 
CTCTTCTGCC GCGATTGCGA ACAGGCCGAG AACTGGATGT CCGCTCGTGA AGCTTTCCTT 
AGTAGCAGTA GCGCTGATGC CCTCGACAGC GGTGACAATG TAGAGGCCCT CATCAAGAAG 
CACGAGGACT TCGACAAGGC TATCAACGGT CACGAAGAGA AAATAGCCGC TCTTCAAACT 
CTGGCTGATC AGCTTATTGC TGCTGAGCAC TACGCTGCTC GACCTATCGA CGAACGTCGC 
TGCCAGGTAC TCGATCGCTG GCGTCACTTG AAAGATGCTC TCATTGAGAA ACGATCACGG 
CTTGGCGAGT CCCAAACTCT GCAGCAATTC TCTCGCGATG CTGATGAGAT GGAGAATTGG 
ATCGCTGAGA AGCTGCAGCT CGCCACTGAA GAGAACTACA AAGATCCGGC TAATATACAG 
TCCAAACATC AAAAACACCA AGCCTTTGAA GCTGAGCTTG CTGCAAATGC CGAACGTATC 
TCGTCGGTGC TGGCTATGGG TGGTAACCTG ATCGAAAAGC ATCAGTGCGC TGGCTCTGAA 
GATGCCGTGC AGAAGCGTCT TGCCTCTATT GCCGATCAGT GGGAGTACCT CACTCACAAA 
ACAACCGAAA AATCCATGAA ACTAAAGGAA GCCAATAAGC AACGCACCTA CATCGCTGCA 
GTCAAGGATT TAGACTTTTG GCTCGGTGAG GTCGAGAGTC TGCTGACAAG CGAAGATGCT 
GGCAAGGATC TCGCCTCAGT GCAGAACCTC ATGAAGAAGC ATCAACTGGT TGAGGCCGAC 
ATCAACGCCC ACGAGGAGCG TATTAAAGAC ATGAATGCTC AGGCTGACTC CCTCATCGAG 
AGCGGTCAAT TCGAGGCAGC GGGTATCCAA GAAAAGCGTC AAAGCATCAA CGAACGCTAT 
GAACGCGTAC GCAACCTCGC AGCTCACAGG CAGGCTAGAC TCAATGAGGC TAACACCTTG 
CATCAGTTCT TCCGCGACAT CGCCGACGAG GAGTCCTGGA TCAAGGAGAA GAAGCTTCTC 
GTTGGTTCAG ATGACTACGG TCGTGACCTT ACAGGCGTTC AGAATTTAAA GAAAAAGCAC 
AAGCGTCTCG AGGCTGAGCT TGGCAGCCAC GAGCCCGCTA TCCAAGCCGT ACAAGAAGCT 
GGTGAGAAAC TCATGGACGT CTCCAACCTA GGCGTACCAG AGATCGAGCA ACGACTCAAG 
CTTCTCGAAC AGGCGTGGTC TGAATTGAAA CAACTAGCTG CCACGCGTGG TCAGAAGCTT 
GACGAGTCGC TGGCTTACCA GCAATTTTTG GCAAAGGTTG AAGAGGAAGA AGCCTGGATC 
GCCGAGAAAC AGCAATTGCT CTCACTCGAG GATTACGGCG ATACAATGGC TGCCGTGCAA 
GGTTTGCTCA AGAAGCACGA CGCTTTCGAG ACAGACTTTG CTGCCCACGG TGAACGTTGC 
CGTGAAATCC GCGAAGCCGG TGAGGGACTC ATAGCTGCAG GTAACCATCG TGCTGAGGCA 
ATCGAACAGC GCTGCCAGCA GCTAGGAGCC AAGCTTGAAC AGCTTGGAGC TTTGGCTGCT 
AGACGCAAGA CTCGCCTCAA CGATAACTCA GCTTACCTGC AGTTCATGTG GAAGGCTGAC 
GTAGTCGAGT CCTGGATCGC TGACAAGGAA ACACACGTAC GATCCGAAGA GTTTGGCCGT 
GATTTGTCCA GTGTGCAGAC TTTGCTCACC AAACAGGAGA CCTTCGACGC TGGTCTGCAC 
GCTTTCGAAC ACGAGGGTAT TCAGAACATA ACTTCTCTCA AGGAGATGCT AATCGAGGCC 
GGTCATGAAC AGAGCCCCGC GATTCACAAA CGTCACGCTG ACGTTATCGC TCGCTGGCAG 
AAGCTGCTTG CCGATTCGCA AGCCCGTAAG CAGCGTCTCT TGCAGATGCA GGAGCAATTC 
CGTCAGATAG AAGAGCTTTA CCTGACTTTC GCCAAGAAGG CATCGGCCTT CAACTCGTGG 
TTCGAAAACG CTGAGGAGGA TTTGACCGAC CCTGTCCGCT GCAACAGCAT CGAAGAGATC 
CGTGCTTTGC GCGAAGCTCA TGCGCAGTTC CAAGCTAGTC TGTCATCGGC TGAGGCCGAC 
TTCCAGGCTC TTGGAGCTCT TGACCGCCAA ATCAAGAACT TCAATGTTGG CCCCAACCCT 
TACACCTGGT TCACCATGGA AGCGCTCGAG GACACCTGGC GCAACTTGCA GAAGATCATC 
AAGGAGCGCG ACGTCGAATT AGCTAAGGAA GCTCAGCGTC AAGAGGAAAA CGACAAGTTG 
CGAAAGGAGT TCGCCAAGCA CGCCAATGCC TTCCATCAGT GGCTCGCGGA CACCCGTACT 
TCTATGATGG AAGGATCGGG CTCACTTGAA CAACAGCTCG AAGCAACAAA GAGAAAGACA 
CAAGAAGTCC GAGCGCGGCG TCAAGATCTC AAAAAGATCG AGGATTTGGG CGCAATACTG 
GAAGAACATT TGATTCTCGA CAACAGGTAC ACAGAACACA GTACTGTAGG ACTGGCCCAA 
CAATGGGACC AGCTCGACCA GTTGGGTATG CGTATGCAGC ATAACCTGGA ACAACAGATT 
CAAGCTCGTA ACCAATCTGG AGTGTCGGAA GGAGCCCTCA AGGAGTTCTC GATGATGTTC 
AAGCATTACG ACAAGGACAA GAGCGGACGA CTAAATCACC AAGAGTTCAA GTCGTGCCTC 
AGAGCTTTGG GTTACGATCT ACCTATGGTG GAAGAAGGAC AACCAGATCC AGAATTCGAA 
AATATTCTAG ACATTGTCGA CCCGAACAGA GATGGCTTTG TTTCCTTACA AGATCACATG 
GCATTCATGA TCAGCAAGGA AACTGAGAAT GTCCAAAGTT CACAAGAAAT CGAAAACGCC 
TTCCGAGCTA TTACAGCTGC CGATCGTCCT TACGTCACGA AAGAGGAACT CTACGCTAAC 
CTCACGAAAG AAATGGCGGA CTATTGTGTG GCGAGGATGA AACCATACGT CGACCCGAAG 
ACGGAACGAC CGATCACCGG CGCACTAGAC TATATAGAAT TTACACACAC GTTATTCCAG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001601352.1
CDS	451..7716
Translation	MDQQITPKEVKILENPEDIQERREQVLGRYANFKSEARSKRDKLEDSRRFQYFKRDADELEGWIYEKLQAASDESYKDPTNLQAKIQKHQAFEAEVAAHSNAIVLLDNTGSEMIAQHHFASDIIRKRLEELHRLWELLLNRLADKGLKLQQALVLVQFIRHCDEVMFWIHDKEVFVTTDEFGQDLEHVEVLQRKFDEFQKDMTSQEYRVTEVNELADKLLLDAHPERDTILRRKEELNESWQRLKQLAILRQEKLFGAHEIQRFNRDADETMAWIGEKDAVLSSDDFGRDLASVQLLQRTHENIERDLAALEDKVATLSTEADRLAGIHQAEHSQQIQSKRAEILQSWESLTAKAKERRFKLDESYYLHRFLADYRDLVSWMNNMRAIISADELAKDVAGAEALLERHQEHKGEIDARADSFDSTGLAGSKLLERQHYAASEVAIKLESLAADKASLLELWEKRRILYEQCMDLQLFYRDTEQADAWMAKQEAFLANEDLGDSLDSVEALIKKHEDFDKSLAAQEEKIKALDEFASKLIEGEHYAADEVAQRRQALLQRRSALLEKSALRRRALEDAYKLQQFERDCDETKGWVNEKLKFATDDSYLDPTNLNGKLQKHQNFEQELQANKTRMQEMVAQGQELIEAEHYARERIQSRIKEIVELWESLTEATEKKGNKLQEAAQQQQFNRTVEDVELWLSEVEGQLLSEDYGKDLTSVQNLQKKHALLEADVASHQERIASIAQAAEQFVAGGHFDADNIRQKQQQLQGRYSALEQPMQMRKQRLLDSLQVQQLFRDVEDEEAWIREKEPVAASTNRGRDLIGVQNLQKKHQAVLAEINNHEPRVAAVCAAGSRMLSDGHFAAEEISQRLASLDEHWGQLKEKARQRKNDLDDSLQAHQYFADANEAESWMKEKRPIVMNADYGKDEDSSEALLKKHEALVSDLEAFASTIAALKEQAAACRQQETPAVDVAGKECVVALYDYTEKSPREVSMKKGDTLTLLNSSNKDWWKVEVNDRQGFVPAAYVKRVEPDAGLSASQQNLAREQGSIAARQAQIEAQYEDLLRLARERQNKLNETAKAYVLVREAAELATWIKGKENHAQVQDVGEDLEQVEVMQKKFDDFRADLKANEVRLAEMNEIAVQLMSVGQTEAALKIQTQIQELNEKWTSLQTLTAERASQLDSAHEVQRFHRDVDDTKDWIREKDAALNNDDLGKDLRSVQTLLRKHEGLERDLAALGDKIKQLDETANRLMQSHPDTAEQTYTKQKEINEEWTQLTAKADSRKKKLLDSYDLQRYLSDYRDLMAWINSMMGLVASEELANDVTGAEALLERHQEHRMEIDARVGTFQAFELFGQQLLQSNHYASVEIQEKLESMNEARQELEKAWIQRRMQLDQNLELQLFCRDCEQAENWMSAREAFLSSSSADALDSGDNVEALIKKHEDFDKAINGHEEKIAALQTLADQLIAAEHYAARPIDERRCQVLDRWRHLKDALIEKRSRLGESQTLQQFSRDADEMENWIAEKLQLATEENYKDPANIQSKHQKHQAFEAELAANAERISSVLAMGGNLIEKHQCAGSEDAVQKRLASIADQWEYLTHKTTEKSMKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADINAHEERIKDMNAQADSLIESGQFEAAGIQEKRQSINERYERVRNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKLLEQAWSELKQLAATRGQKLDESLAYQQFLAKVEEEEAWIAEKQQLLSLEDYGDTMAAVQGLLKKHDAFETDFAAHGERCREIREAGEGLIAAGNHRAEAIEQRCQQLGAKLEQLGALAARRKTRLNDNSAYLQFMWKADVVESWIADKETHVRSEEFGRDLSSVQTLLTKQETFDAGLHAFEHEGIQNITSLKEMLIEAGHEQSPAIHKRHADVIARWQKLLADSQARKQRLLQMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALREAHAQFQASLSSAEADFQALGALDRQIKNFNVGPNPYTWFTMEALEDTWRNLQKIIKERDVELAKEAQRQEENDKLRKEFAKHANAFHQWLADTRTSMMEGSGSLEQQLEATKRKTQEVRARRQDLKKIEDLGAILEEHLILDNRYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNQSGVSEGALKEFSMMFKHYDKDKSGRLNHQEFKSCLRALGYDLPMVEEGQPDPEFENILDIVDPNRDGFVSLQDHMAFMISKETENVQSSQEIENAFRAITAADRPYVTKEELYANLTKEMADYCVARMKPYVDPKTERPITGALDYIEFTHTLFQN

Target ORF information:

RefSeq Version	XM_001601302.4
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001601302.4

ORF Insert Sequence:

ATGGATCAGC AGATCACCCC CAAGGAGGTG AAAATCCTCG AGAACCCAGA GGACATCCAG 
GAGAGGAGAG AGCAGGTTCT GGGACGTTAT GCCAATTTTA AGTCGGAGGC GCGCAGCAAG 
CGCGACAAAC TCGAGGACTC TCGTCGTTTC CAGTACTTTA AACGAGATGC AGATGAACTC 
GAGGGTTGGA TTTATGAGAA GTTGCAAGCT GCTTCCGATG AAAGCTACAA AGATCCGACC 
AACCTACAGG CAAAGATTCA AAAGCACCAG GCTTTTGAAG CCGAAGTCGC TGCTCATTCG 
AATGCGATTG TTCTATTAGA CAATACTGGA TCGGAAATGA TTGCTCAGCA CCACTTTGCT 
AGTGACATCA TTCGTAAAAG GCTTGAGGAA TTGCATCGCT TATGGGAATT GCTTCTTAAT 
CGACTGGCTG ACAAGGGATT GAAGCTGCAG CAGGCTTTGG TTCTTGTACA ATTCATTCGC 
CATTGCGATG AAGTCATGTT TTGGATACAT GACAAGGAAG TCTTCGTCAC CACTGATGAG 
TTTGGACAAG ATTTGGAGCA CGTAGAAGTA CTCCAAAGGA AATTTGACGA ATTCCAGAAA 
GACATGACCA GCCAAGAATA TCGTGTAACT GAAGTTAACG AATTAGCCGA TAAATTACTT 
TTGGACGCTC ATCCTGAGCG CGATACTATT TTAAGACGCA AAGAAGAATT GAATGAATCA 
TGGCAGCGAT TGAAACAACT CGCTATTCTG CGTCAAGAAA AATTGTTCGG TGCTCACGAA 
ATTCAGAGAT TCAATCGTGA TGCCGACGAG ACAATGGCTT GGATTGGAGA GAAAGACGCA 
GTACTGTCTT CCGATGATTT TGGTCGCGAT TTGGCTAGTG TACAGCTGCT CCAGCGTACT 
CACGAGAACA TTGAACGCGA TTTGGCTGCG CTCGAAGACA AGGTGGCTAC GCTTAGCACT 
GAAGCTGACA GACTAGCCGG CATCCATCAA GCCGAGCACT CTCAGCAAAT TCAGTCCAAG 
AGAGCAGAGA TTCTACAGTC CTGGGAATCG CTTACTGCTA AGGCAAAAGA ACGCCGTTTC 
AAGCTCGACG AGTCTTACTA CTTACATCGC TTCCTTGCTG ACTACCGCGA CCTTGTTTCA 
TGGATGAACA ACATGCGAGC AATCATCTCA GCTGATGAGC TTGCAAAGGA CGTGGCTGGA 
GCTGAGGCGC TTCTTGAACG TCACCAAGAG CACAAGGGCG AGATCGATGC TCGTGCCGAC 
AGCTTTGATT CAACCGGCCT CGCCGGAAGT AAGCTTCTCG AACGTCAACA CTACGCTGCG 
TCGGAGGTAG CCATCAAGCT CGAATCTCTC GCTGCTGACA AGGCTTCGTT GCTTGAACTT 
TGGGAAAAGC GACGCATTCT TTACGAGCAG TGCATGGATC TGCAGCTCTT TTACCGTGAC 
ACTGAACAAG CTGATGCCTG GATGGCCAAG CAGGAAGCTT TCCTCGCCAA CGAGGATCTT 
GGAGATTCCC TTGACAGCGT CGAGGCTCTC ATCAAGAAGC ACGAGGACTT TGACAAGTCC 
CTAGCTGCCC AGGAGGAAAA GATCAAGGCG CTTGACGAGT TTGCCAGCAA GCTCATCGAA 
GGCGAACATT ATGCGGCCGA TGAAGTAGCC CAGCGCCGGC AGGCTCTTTT GCAACGTCGC 
TCAGCCCTCC TTGAAAAGTC GGCTTTGCGC CGTCGTGCCC TTGAGGACGC CTACAAGCTG 
CAGCAGTTCG AGCGTGACTG CGATGAGACC AAAGGCTGGG TCAACGAGAA GCTTAAGTTC 
GCCACCGATG ACAGCTACCT CGACCCTACC AATCTCAATG GTAAGCTGCA GAAACACCAG 
AATTTCGAGC AGGAGCTTCA AGCAAACAAA ACGCGCATGC AAGAAATGGT CGCTCAGGGC 
CAGGAACTCA TCGAAGCCGA GCATTACGCT CGCGAGCGCA TCCAGTCGCG CATCAAGGAG 
ATCGTCGAGC TCTGGGAAAG TCTGACCGAA GCCACTGAGA AGAAGGGCAA CAAGCTTCAG 
GAAGCTGCGC AACAACAGCA ATTCAACCGC ACTGTCGAGG ACGTCGAACT CTGGCTCTCA 
GAGGTCGAAG GTCAGCTCTT GTCCGAAGAC TATGGCAAGG ACCTTACCAG CGTGCAGAAC 
CTTCAGAAAA AGCACGCCCT GCTCGAGGCC GACGTCGCCT CCCATCAAGA GCGTATCGCC 
TCTATTGCCC AGGCAGCCGA GCAGTTCGTC GCTGGAGGTC ACTTTGACGC CGACAACATC 
CGTCAGAAGC AGCAACAACT GCAGGGACGC TATTCTGCTT TGGAGCAGCC TATGCAGATG 
CGCAAGCAGC GCTTGCTGGA CTCGCTGCAG GTACAGCAGC TGTTCCGCGA TGTCGAAGAC 
GAGGAGGCCT GGATCCGCGA GAAGGAGCCT GTGGCAGCCA GCACCAACCG TGGTCGCGAT 
CTCATTGGTG TGCAGAACCT GCAAAAGAAG CACCAGGCCG TCCTTGCTGA AATCAACAAT 
CATGAGCCCC GAGTTGCAGC CGTCTGCGCA GCTGGCTCAC GCATGTTGAG CGATGGTCAT 
TTCGCAGCTG AAGAGATCAG CCAGAGACTG GCTAGTCTCG ATGAACATTG GGGACAGTTG 
AAGGAGAAGG CTCGCCAGCG TAAGAACGAC CTTGATGACT CGCTCCAGGC TCACCAATAC 
TTCGCCGATG CCAATGAAGC CGAGTCTTGG ATGAAGGAGA AGCGCCCCAT CGTCATGAAC 
GCCGATTACG GCAAAGACGA GGATAGTAGC GAGGCTCTGC TCAAGAAACA CGAGGCTCTC 
GTGAGCGATC TCGAAGCTTT TGCTAGCACC ATTGCAGCGC TCAAGGAACA GGCAGCCGCC 
TGCCGTCAGC AAGAGACACC TGCTGTCGAC GTAGCTGGAA AGGAATGCGT TGTCGCACTC 
TACGATTACA CCGAGAAGTC TCCTCGCGAG GTCTCTATGA AGAAAGGAGA CACCCTTACA 
CTCCTCAACA GCAGCAACAA GGACTGGTGG AAGGTGGAGG TAAACGATCG TCAGGGCTTC 
GTGCCAGCCG CCTATGTCAA GCGCGTAGAA CCGGACGCGG GTCTTAGTGC TTCTCAGCAG 
AATCTGGCTC GCGAGCAGGG CTCCATTGCC GCTCGCCAGG CCCAGATTGA AGCCCAATAC 
GAAGACTTGC TGCGATTGGC CAGAGAAAGA CAAAACAAGC TTAATGAAAC CGCCAAGGCG 
TACGTGTTGG TTCGCGAGGC CGCTGAACTG GCCACCTGGA TCAAGGGCAA GGAGAACCAT 
GCTCAGGTAC AGGATGTAGG CGAGGATTTG GAACAAGTCG AGGTTATGCA GAAGAAGTTC 
GACGATTTCC GAGCTGATCT GAAGGCAAAC GAGGTGCGTC TCGCAGAGAT GAACGAGATC 
GCCGTGCAGC TGATGAGCGT CGGCCAGACC GAGGCAGCTC TTAAGATCCA GACGCAGATC 
CAAGAGCTTA ACGAAAAATG GACGAGTCTG CAGACTCTAA CAGCCGAGCG CGCCAGTCAG 
CTGGACTCTG CTCACGAAGT ACAGCGCTTC CATCGCGATG TCGACGACAC CAAAGACTGG 
ATTCGCGAGA AGGATGCTGC CCTGAACAAC GATGACCTGG GCAAGGACTT GCGCAGCGTG 
CAAACTCTGC TCCGCAAGCA CGAAGGTCTG GAGCGCGACC TCGCTGCCCT TGGGGACAAG 
ATTAAGCAGT TGGACGAAAC AGCAAACCGC TTGATGCAGT CGCATCCTGA TACGGCCGAG 
CAAACCTACA CCAAGCAGAA GGAGATCAAC GAGGAATGGA CTCAGCTTAC TGCCAAAGCT 
GATTCTAGAA AGAAGAAGCT GCTCGATTCT TATGACCTGC AACGCTATCT CAGCGACTAT 
CGGGATCTTA TGGCCTGGAT TAATTCGATG ATGGGTCTGG TAGCTTCCGA AGAATTGGCT 
AACGACGTCA CCGGTGCTGA GGCTCTGCTT GAACGTCACC AGGAACATCG CATGGAAATC 
GACGCCCGCG TTGGAACTTT CCAAGCTTTC GAGCTCTTCG GCCAGCAACT CTTGCAATCC 
AACCACTATG CTAGCGTTGA GATACAAGAG AAACTCGAAT CTATGAACGA GGCTCGTCAA 
GAACTTGAGA AGGCTTGGAT ACAGCGCCGT ATGCAGTTGG ACCAGAACCT CGAGCTGCAA 
CTCTTCTGCC GCGATTGCGA ACAGGCCGAG AACTGGATGT CCGCTCGTGA AGCTTTCCTT 
AGTAGCAGTA GCGCTGATGC CCTCGACAGC GGTGACAATG TAGAGGCCCT CATCAAGAAG 
CACGAGGACT TCGACAAGGC TATCAACGGT CACGAAGAGA AAATAGCCGC TCTTCAAACT 
CTGGCTGATC AGCTTATTGC TGCTGAGCAC TACGCTGCTC GACCTATCGA CGAACGTCGC 
TGCCAGGTAC TCGATCGCTG GCGTCACTTG AAAGATGCTC TCATTGAGAA ACGATCACGG 
CTTGGCGAGT CCCAAACTCT GCAGCAATTC TCTCGCGATG CTGATGAGAT GGAGAATTGG 
ATCGCTGAGA AGCTGCAGCT CGCCACTGAA GAGAACTACA AAGATCCGGC TAATATACAG 
TCCAAACATC AAAAACACCA AGCCTTTGAA GCTGAGCTTG CTGCAAATGC CGAACGTATC 
TCGTCGGTGC TGGCTATGGG TGGTAACCTG ATCGAAAAGC ATCAGTGCGC TGGCTCTGAA 
GATGCCGTGC AGAAGCGTCT TGCCTCTATT GCCGATCAGT GGGAGTACCT CACTCACAAA 
ACAACCGAAA AATCCATGAA ACTAAAGGAA GCCAATAAGC AACGCACCTA CATCGCTGCA 
GTCAAGGATT TAGACTTTTG GCTCGGTGAG GTCGAGAGTC TGCTGACAAG CGAAGATGCT 
GGCAAGGATC TCGCCTCAGT GCAGAACCTC ATGAAGAAGC ATCAACTGGT TGAGGCCGAC 
ATCAACGCCC ACGAGGAGCG TATTAAAGAC ATGAATGCTC AGGCTGACTC CCTCATCGAG 
AGCGGTCAAT TCGAGGCAGC GGGTATCCAA GAAAAGCGTC AAAGCATCAA CGAACGCTAT 
GAACGCGTAC GCAACCTCGC AGCTCACAGG CAGGCTAGAC TCAATGAGGC TAACACCTTG 
CATCAGTTCT TCCGCGACAT CGCCGACGAG GAGTCCTGGA TCAAGGAGAA GAAGCTTCTC 
GTTGGTTCAG ATGACTACGG TCGTGACCTT ACAGGCGTTC AGAATTTAAA GAAAAAGCAC 
AAGCGTCTCG AGGCTGAGCT TGGCAGCCAC GAGCCCGCTA TCCAAGCCGT ACAAGAAGCT 
GGTGAGAAAC TCATGGACGT CTCCAACCTA GGCGTACCAG AGATCGAGCA ACGACTCAAG 
CTTCTCGAAC AGGCGTGGTC TGAATTGAAA CAACTAGCTG CCACGCGTGG TCAGAAGCTT 
GACGAGTCGC TGGCTTACCA GCAATTTTTG GCAAAGGTTG AAGAGGAAGA AGCCTGGATC 
GCCGAGAAAC AGCAATTGCT CTCACTCGAG GATTACGGCG ATACAATGGC TGCCGTGCAA 
GGTTTGCTCA AGAAGCACGA CGCTTTCGAG ACAGACTTTG CTGCCCACGG TGAACGTTGC 
CGTGAAATCC GCGAAGCCGG TGAGGGACTC ATAGCTGCAG GTAACCATCG TGCTGAGGCA 
ATCGAACAGC GCTGCCAGCA GCTAGGAGCC AAGCTTGAAC AGCTTGGAGC TTTGGCTGCT 
AGACGCAAGA CTCGCCTCAA CGATAACTCA GCTTACCTGC AGTTCATGTG GAAGGCTGAC 
GTAGTCGAGT CCTGGATCGC TGACAAGGAA ACACACGTAC GATCCGAAGA GTTTGGCCGT 
GATTTGTCCA GTGTGCAGAC TTTGCTCACC AAACAGGAGA CCTTCGACGC TGGTCTGCAC 
GCTTTCGAAC ACGAGGGTAT TCAGAACATA ACTTCTCTCA AGGAGATGCT AATCGAGGCC 
GGTCATGAAC AGAGCCCCGC GATTCACAAA CGTCACGCTG ACGTTATCGC TCGCTGGCAG 
AAGCTGCTTG CCGATTCGCA AGCCCGTAAG CAGCGTCTCT TGCAGATGCA GGAGCAATTC 
CGTCAGATAG AAGAGCTTTA CCTGACTTTC GCCAAGAAGG CATCGGCCTT CAACTCGTGG 
TTCGAAAACG CTGAGGAGGA TTTGACCGAC CCTGTCCGCT GCAACAGCAT CGAAGAGATC 
CGTGCTTTGC GCGAAGCTCA TGCGCAGTTC CAAGCTAGTC TGTCATCGGC TGAGGCCGAC 
TTCCAGGCTC TTGGAGCTCT TGACCGCCAA ATCAAGAACT TCAATGTTGG CCCCAACCCT 
TACACCTGGT TCACCATGGA AGCGCTCGAG GACACCTGGC GCAACTTGCA GAAGATCATC 
AAGGAGCGCG ACGTCGAATT AGCTAAGGAA GCTCAGCGTC AAGAGGAAAA CGACAAGTTG 
CGAAAGGAGT TCGCCAAGCA CGCCAATGCC TTCCATCAGT GGCTCGCGGA CACCCGTACT 
TCTATGATGG AAGGATCGGG CTCACTTGAA CAACAGCTCG AAGCAACAAA GAGAAAGACA 
CAAGAAGTCC GAGCGCGGCG TCAAGATCTC AAAAAGATCG AGGATTTGGG CGCAATACTG 
GAAGAACATT TGATTCTCGA CAACAGGTAC ACAGAACACA GTACTGTAGG ACTGGCCCAA 
CAATGGGACC AGCTCGACCA GTTGGGTATG CGTATGCAGC ATAACCTGGA ACAACAGATT 
CAAGCTCGTA ACCAATCTGG AGTGTCGGAA GGAGCCCTCA AGGAGTTCTC GATGATGTTC 
AAGCATTACG ACAAGGACAA GAGCGGACGA CTAAATCACC AAGAGTTCAA GTCGTGCCTC 
AGAGCTTTGG GTTACGATCT ACCTATGGTG GAAGAAGGAC AACCAGATCC AGAATTCGAA 
AATATTCTAG ACATTGTCGA CCCGAACAGA GATGGCTTTG TTTCCTTACA AGATCACATG 
GCATTCATGA TCAGCAAGGA AACTGAGAAT GTCCAAAGTT CACAAGAAAT CGAAAACGCC 
TTCCGAGCTA TTACAGCTGC CGATCGTCCT TACGTCACGA AAGAGGAACT CTACGCTAAC 
CTCACGAAAG AAATGGCGGA CTATTGTGTG GCGAGGATGA AACCATACGT CGACCCGAAG 
ACGGAACGAC CGATCACCGG CGCACTAGAC TATATAGAAT TTACACACAC GTTATTCCAG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100116996 ( XM_031922932.1 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_031778792.1 Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X2, mRNA.

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CloneID	ONd04093
Clone ID Related Accession (Same CDS sequence)	XM_001601302.4 , XM_031922932.1
Accession Version	XM_031922932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577030400000
Organism	Nasonia vitripennis(jewel wasp)
Product	spectrin alpha chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022279606.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nasonia vitripennis Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGC AGATCACCCC CAAGGAGGTG AAAATCCTCG AGAACCCAGA GGACATCCAG 
GAGAGGAGAG AGCAGGTTCT GGGACGTTAT GCCAATTTTA AGTCGGAGGC GCGCAGCAAG 
CGCGACAAAC TCGAGGACTC TCGTCGTTTC CAGTACTTTA AACGAGATGC AGATGAACTC 
GAGGGTTGGA TTTATGAGAA GTTGCAAGCT GCTTCCGATG AAAGCTACAA AGATCCGACC 
AACCTACAGG CAAAGATTCA AAAGCACCAG GCTTTTGAAG CCGAAGTCGC TGCTCATTCG 
AATGCGATTG TTCTATTAGA CAATACTGGA TCGGAAATGA TTGCTCAGCA CCACTTTGCT 
AGTGACATCA TTCGTAAAAG GCTTGAGGAA TTGCATCGCT TATGGGAATT GCTTCTTAAT 
CGACTGGCTG ACAAGGGATT GAAGCTGCAG CAGGCTTTGG TTCTTGTACA ATTCATTCGC 
CATTGCGATG AAGTCATGTT TTGGATACAT GACAAGGAAG TCTTCGTCAC CACTGATGAG 
TTTGGACAAG ATTTGGAGCA CGTAGAAGTA CTCCAAAGGA AATTTGACGA ATTCCAGAAA 
GACATGACCA GCCAAGAATA TCGTGTAACT GAAGTTAACG AATTAGCCGA TAAATTACTT 
TTGGACGCTC ATCCTGAGCG CGATACTATT TTAAGACGCA AAGAAGAATT GAATGAATCA 
TGGCAGCGAT TGAAACAACT CGCTATTCTG CGTCAAGAAA AATTGTTCGG TGCTCACGAA 
ATTCAGAGAT TCAATCGTGA TGCCGACGAG ACAATGGCTT GGATTGGAGA GAAAGACGCA 
GTACTGTCTT CCGATGATTT TGGTCGCGAT TTGGCTAGTG TACAGCTGCT CCAGCGTACT 
CACGAGAACA TTGAACGCGA TTTGGCTGCG CTCGAAGACA AGGTGGCTAC GCTTAGCACT 
GAAGCTGACA GACTAGCCGG CATCCATCAA GCCGAGCACT CTCAGCAAAT TCAGTCCAAG 
AGAGCAGAGA TTCTACAGTC CTGGGAATCG CTTACTGCTA AGGCAAAAGA ACGCCGTTTC 
AAGCTCGACG AGTCTTACTA CTTACATCGC TTCCTTGCTG ACTACCGCGA CCTTGTTTCA 
TGGATGAACA ACATGCGAGC AATCATCTCA GCTGATGAGC TTGCAAAGGA CGTGGCTGGA 
GCTGAGGCGC TTCTTGAACG TCACCAAGAG CACAAGGGCG AGATCGATGC TCGTGCCGAC 
AGCTTTGATT CAACCGGCCT CGCCGGAAGT AAGCTTCTCG AACGTCAACA CTACGCTGCG 
TCGGAGGTAG CCATCAAGCT CGAATCTCTC GCTGCTGACA AGGCTTCGTT GCTTGAACTT 
TGGGAAAAGC GACGCATTCT TTACGAGCAG TGCATGGATC TGCAGCTCTT TTACCGTGAC 
ACTGAACAAG CTGATGCCTG GATGGCCAAG CAGGAAGCTT TCCTCGCCAA CGAGGATCTT 
GGAGATTCCC TTGACAGCGT CGAGGCTCTC ATCAAGAAGC ACGAGGACTT TGACAAGTCC 
CTAGCTGCCC AGGAGGAAAA GATCAAGGCG CTTGACGAGT TTGCCAGCAA GCTCATCGAA 
GGCGAACATT ATGCGGCCGA TGAAGTAGCC CAGCGCCGGC AGGCTCTTTT GCAACGTCGC 
TCAGCCCTCC TTGAAAAGTC GGCTTTGCGC CGTCGTGCCC TTGAGGACGC CTACAAGCTG 
CAGCAGTTCG AGCGTGACTG CGATGAGACC AAAGGCTGGG TCAACGAGAA GCTTAAGTTC 
GCCACCGATG ACAGCTACCT CGACCCTACC AATCTCAATG GTAAGCTGCA GAAACACCAG 
AATTTCGAGC AGGAGCTTCA AGCAAACAAA ACGCGCATGC AAGAAATGGT CGCTCAGGGC 
CAGGAACTCA TCGAAGCCGA GCATTACGCT CGCGAGCGCA TCCAGTCGCG CATCAAGGAG 
ATCGTCGAGC TCTGGGAAAG TCTGACCGAA GCCACTGAGA AGAAGGGCAA CAAGCTTCAG 
GAAGCTGCGC AACAACAGCA ATTCAACCGC ACTGTCGAGG ACGTCGAACT CTGGCTCTCA 
GAGGTCGAAG GTCAGCTCTT GTCCGAAGAC TATGGCAAGG ACCTTACCAG CGTGCAGAAC 
CTTCAGAAAA AGCACGCCCT GCTCGAGGCC GACGTCGCCT CCCATCAAGA GCGTATCGCC 
TCTATTGCCC AGGCAGCCGA GCAGTTCGTC GCTGGAGGTC ACTTTGACGC CGACAACATC 
CGTCAGAAGC AGCAACAACT GCAGGGACGC TATTCTGCTT TGGAGCAGCC TATGCAGATG 
CGCAAGCAGC GCTTGCTGGA CTCGCTGCAG GTACAGCAGC TGTTCCGCGA TGTCGAAGAC 
GAGGAGGCCT GGATCCGCGA GAAGGAGCCT GTGGCAGCCA GCACCAACCG TGGTCGCGAT 
CTCATTGGTG TGCAGAACCT GCAAAAGAAG CACCAGGCCG TCCTTGCTGA AATCAACAAT 
CATGAGCCCC GAGTTGCAGC CGTCTGCGCA GCTGGCTCAC GCATGTTGAG CGATGGTCAT 
TTCGCAGCTG AAGAGATCAG CCAGAGACTG GCTAGTCTCG ATGAACATTG GGGACAGTTG 
AAGGAGAAGG CTCGCCAGCG TAAGAACGAC CTTGATGACT CGCTCCAGGC TCACCAATAC 
TTCGCCGATG CCAATGAAGC CGAGTCTTGG ATGAAGGAGA AGCGCCCCAT CGTCATGAAC 
GCCGATTACG GCAAAGACGA GGATAGTAGC GAGGCTCTGC TCAAGAAACA CGAGGCTCTC 
GTGAGCGATC TCGAAGCTTT TGCTAGCACC ATTGCAGCGC TCAAGGAACA GGCAGCCGCC 
TGCCGTCAGC AAGAGACACC TGCTGTCGAC GTAGCTGGAA AGGAATGCGT TGTCGCACTC 
TACGATTACA CCGAGAAGTC TCCTCGCGAG GTCTCTATGA AGAAAGGAGA CACCCTTACA 
CTCCTCAACA GCAGCAACAA GGACTGGTGG AAGGTGGAGG TAAACGATCG TCAGGGCTTC 
GTGCCAGCCG CCTATGTCAA GCGCGTAGAA CCGGACGCGG GTCTTAGTGC TTCTCAGCAG 
AATCTGGCTC GCGAGCAGGG CTCCATTGCC GCTCGCCAGG CCCAGATTGA AGCCCAATAC 
GAAGACTTGC TGCGATTGGC CAGAGAAAGA CAAAACAAGC TTAATGAAAC CGCCAAGGCG 
TACGTGTTGG TTCGCGAGGC CGCTGAACTG GCCACCTGGA TCAAGGGCAA GGAGAACCAT 
GCTCAGGTAC AGGATGTAGG CGAGGATTTG GAACAAGTCG AGGTTATGCA GAAGAAGTTC 
GACGATTTCC GAGCTGATCT GAAGGCAAAC GAGGTGCGTC TCGCAGAGAT GAACGAGATC 
GCCGTGCAGC TGATGAGCGT CGGCCAGACC GAGGCAGCTC TTAAGATCCA GACGCAGATC 
CAAGAGCTTA ACGAAAAATG GACGAGTCTG CAGACTCTAA CAGCCGAGCG CGCCAGTCAG 
CTGGACTCTG CTCACGAAGT ACAGCGCTTC CATCGCGATG TCGACGACAC CAAAGACTGG 
ATTCGCGAGA AGGATGCTGC CCTGAACAAC GATGACCTGG GCAAGGACTT GCGCAGCGTG 
CAAACTCTGC TCCGCAAGCA CGAAGGTCTG GAGCGCGACC TCGCTGCCCT TGGGGACAAG 
ATTAAGCAGT TGGACGAAAC AGCAAACCGC TTGATGCAGT CGCATCCTGA TACGGCCGAG 
CAAACCTACA CCAAGCAGAA GGAGATCAAC GAGGAATGGA CTCAGCTTAC TGCCAAAGCT 
GATTCTAGAA AGAAGAAGCT GCTCGATTCT TATGACCTGC AACGCTATCT CAGCGACTAT 
CGGGATCTTA TGGCCTGGAT TAATTCGATG ATGGGTCTGG TAGCTTCCGA AGAATTGGCT 
AACGACGTCA CCGGTGCTGA GGCTCTGCTT GAACGTCACC AGGAACATCG CATGGAAATC 
GACGCCCGCG TTGGAACTTT CCAAGCTTTC GAGCTCTTCG GCCAGCAACT CTTGCAATCC 
AACCACTATG CTAGCGTTGA GATACAAGAG AAACTCGAAT CTATGAACGA GGCTCGTCAA 
GAACTTGAGA AGGCTTGGAT ACAGCGCCGT ATGCAGTTGG ACCAGAACCT CGAGCTGCAA 
CTCTTCTGCC GCGATTGCGA ACAGGCCGAG AACTGGATGT CCGCTCGTGA AGCTTTCCTT 
AGTAGCAGTA GCGCTGATGC CCTCGACAGC GGTGACAATG TAGAGGCCCT CATCAAGAAG 
CACGAGGACT TCGACAAGGC TATCAACGGT CACGAAGAGA AAATAGCCGC TCTTCAAACT 
CTGGCTGATC AGCTTATTGC TGCTGAGCAC TACGCTGCTC GACCTATCGA CGAACGTCGC 
TGCCAGGTAC TCGATCGCTG GCGTCACTTG AAAGATGCTC TCATTGAGAA ACGATCACGG 
CTTGGCGAGT CCCAAACTCT GCAGCAATTC TCTCGCGATG CTGATGAGAT GGAGAATTGG 
ATCGCTGAGA AGCTGCAGCT CGCCACTGAA GAGAACTACA AAGATCCGGC TAATATACAG 
TCCAAACATC AAAAACACCA AGCCTTTGAA GCTGAGCTTG CTGCAAATGC CGAACGTATC 
TCGTCGGTGC TGGCTATGGG TGGTAACCTG ATCGAAAAGC ATCAGTGCGC TGGCTCTGAA 
GATGCCGTGC AGAAGCGTCT TGCCTCTATT GCCGATCAGT GGGAGTACCT CACTCACAAA 
ACAACCGAAA AATCCATGAA ACTAAAGGAA GCCAATAAGC AACGCACCTA CATCGCTGCA 
GTCAAGGATT TAGACTTTTG GCTCGGTGAG GTCGAGAGTC TGCTGACAAG CGAAGATGCT 
GGCAAGGATC TCGCCTCAGT GCAGAACCTC ATGAAGAAGC ATCAACTGGT TGAGGCCGAC 
ATCAACGCCC ACGAGGAGCG TATTAAAGAC ATGAATGCTC AGGCTGACTC CCTCATCGAG 
AGCGGTCAAT TCGAGGCAGC GGGTATCCAA GAAAAGCGTC AAAGCATCAA CGAACGCTAT 
GAACGCGTAC GCAACCTCGC AGCTCACAGG CAGGCTAGAC TCAATGAGGC TAACACCTTG 
CATCAGTTCT TCCGCGACAT CGCCGACGAG GAGTCCTGGA TCAAGGAGAA GAAGCTTCTC 
GTTGGTTCAG ATGACTACGG TCGTGACCTT ACAGGCGTTC AGAATTTAAA GAAAAAGCAC 
AAGCGTCTCG AGGCTGAGCT TGGCAGCCAC GAGCCCGCTA TCCAAGCCGT ACAAGAAGCT 
GGTGAGAAAC TCATGGACGT CTCCAACCTA GGCGTACCAG AGATCGAGCA ACGACTCAAG 
CTTCTCGAAC AGGCGTGGTC TGAATTGAAA CAACTAGCTG CCACGCGTGG TCAGAAGCTT 
GACGAGTCGC TGGCTTACCA GCAATTTTTG GCAAAGGTTG AAGAGGAAGA AGCCTGGATC 
GCCGAGAAAC AGCAATTGCT CTCACTCGAG GATTACGGCG ATACAATGGC TGCCGTGCAA 
GGTTTGCTCA AGAAGCACGA CGCTTTCGAG ACAGACTTTG CTGCCCACGG TGAACGTTGC 
CGTGAAATCC GCGAAGCCGG TGAGGGACTC ATAGCTGCAG GTAACCATCG TGCTGAGGCA 
ATCGAACAGC GCTGCCAGCA GCTAGGAGCC AAGCTTGAAC AGCTTGGAGC TTTGGCTGCT 
AGACGCAAGA CTCGCCTCAA CGATAACTCA GCTTACCTGC AGTTCATGTG GAAGGCTGAC 
GTAGTCGAGT CCTGGATCGC TGACAAGGAA ACACACGTAC GATCCGAAGA GTTTGGCCGT 
GATTTGTCCA GTGTGCAGAC TTTGCTCACC AAACAGGAGA CCTTCGACGC TGGTCTGCAC 
GCTTTCGAAC ACGAGGGTAT TCAGAACATA ACTTCTCTCA AGGAGATGCT AATCGAGGCC 
GGTCATGAAC AGAGCCCCGC GATTCACAAA CGTCACGCTG ACGTTATCGC TCGCTGGCAG 
AAGCTGCTTG CCGATTCGCA AGCCCGTAAG CAGCGTCTCT TGCAGATGCA GGAGCAATTC 
CGTCAGATAG AAGAGCTTTA CCTGACTTTC GCCAAGAAGG CATCGGCCTT CAACTCGTGG 
TTCGAAAACG CTGAGGAGGA TTTGACCGAC CCTGTCCGCT GCAACAGCAT CGAAGAGATC 
CGTGCTTTGC GCGAAGCTCA TGCGCAGTTC CAAGCTAGTC TGTCATCGGC TGAGGCCGAC 
TTCCAGGCTC TTGGAGCTCT TGACCGCCAA ATCAAGAACT TCAATGTTGG CCCCAACCCT 
TACACCTGGT TCACCATGGA AGCGCTCGAG GACACCTGGC GCAACTTGCA GAAGATCATC 
AAGGAGCGCG ACGTCGAATT AGCTAAGGAA GCTCAGCGTC AAGAGGAAAA CGACAAGTTG 
CGAAAGGAGT TCGCCAAGCA CGCCAATGCC TTCCATCAGT GGCTCGCGGA CACCCGTACT 
TCTATGATGG AAGGATCGGG CTCACTTGAA CAACAGCTCG AAGCAACAAA GAGAAAGACA 
CAAGAAGTCC GAGCGCGGCG TCAAGATCTC AAAAAGATCG AGGATTTGGG CGCAATACTG 
GAAGAACATT TGATTCTCGA CAACAGGTAC ACAGAACACA GTACTGTAGG ACTGGCCCAA 
CAATGGGACC AGCTCGACCA GTTGGGTATG CGTATGCAGC ATAACCTGGA ACAACAGATT 
CAAGCTCGTA ACCAATCTGG AGTGTCGGAA GGAGCCCTCA AGGAGTTCTC GATGATGTTC 
AAGCATTACG ACAAGGACAA GAGCGGACGA CTAAATCACC AAGAGTTCAA GTCGTGCCTC 
AGAGCTTTGG GTTACGATCT ACCTATGGTG GAAGAAGGAC AACCAGATCC AGAATTCGAA 
AATATTCTAG ACATTGTCGA CCCGAACAGA GATGGCTTTG TTTCCTTACA AGATCACATG 
GCATTCATGA TCAGCAAGGA AACTGAGAAT GTCCAAAGTT CACAAGAAAT CGAAAACGCC 
TTCCGAGCTA TTACAGCTGC CGATCGTCCT TACGTCACGA AAGAGGAACT CTACGCTAAC 
CTCACGAAAG AAATGGCGGA CTATTGTGTG GCGAGGATGA AACCATACGT CGACCCGAAG 
ACGGAACGAC CGATCACCGG CGCACTAGAC TATATAGAAT TTACACACAC GTTATTCCAG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031778792.1
CDS	1..7266
Translation	MDQQITPKEVKILENPEDIQERREQVLGRYANFKSEARSKRDKLEDSRRFQYFKRDADELEGWIYEKLQAASDESYKDPTNLQAKIQKHQAFEAEVAAHSNAIVLLDNTGSEMIAQHHFASDIIRKRLEELHRLWELLLNRLADKGLKLQQALVLVQFIRHCDEVMFWIHDKEVFVTTDEFGQDLEHVEVLQRKFDEFQKDMTSQEYRVTEVNELADKLLLDAHPERDTILRRKEELNESWQRLKQLAILRQEKLFGAHEIQRFNRDADETMAWIGEKDAVLSSDDFGRDLASVQLLQRTHENIERDLAALEDKVATLSTEADRLAGIHQAEHSQQIQSKRAEILQSWESLTAKAKERRFKLDESYYLHRFLADYRDLVSWMNNMRAIISADELAKDVAGAEALLERHQEHKGEIDARADSFDSTGLAGSKLLERQHYAASEVAIKLESLAADKASLLELWEKRRILYEQCMDLQLFYRDTEQADAWMAKQEAFLANEDLGDSLDSVEALIKKHEDFDKSLAAQEEKIKALDEFASKLIEGEHYAADEVAQRRQALLQRRSALLEKSALRRRALEDAYKLQQFERDCDETKGWVNEKLKFATDDSYLDPTNLNGKLQKHQNFEQELQANKTRMQEMVAQGQELIEAEHYARERIQSRIKEIVELWESLTEATEKKGNKLQEAAQQQQFNRTVEDVELWLSEVEGQLLSEDYGKDLTSVQNLQKKHALLEADVASHQERIASIAQAAEQFVAGGHFDADNIRQKQQQLQGRYSALEQPMQMRKQRLLDSLQVQQLFRDVEDEEAWIREKEPVAASTNRGRDLIGVQNLQKKHQAVLAEINNHEPRVAAVCAAGSRMLSDGHFAAEEISQRLASLDEHWGQLKEKARQRKNDLDDSLQAHQYFADANEAESWMKEKRPIVMNADYGKDEDSSEALLKKHEALVSDLEAFASTIAALKEQAAACRQQETPAVDVAGKECVVALYDYTEKSPREVSMKKGDTLTLLNSSNKDWWKVEVNDRQGFVPAAYVKRVEPDAGLSASQQNLAREQGSIAARQAQIEAQYEDLLRLARERQNKLNETAKAYVLVREAAELATWIKGKENHAQVQDVGEDLEQVEVMQKKFDDFRADLKANEVRLAEMNEIAVQLMSVGQTEAALKIQTQIQELNEKWTSLQTLTAERASQLDSAHEVQRFHRDVDDTKDWIREKDAALNNDDLGKDLRSVQTLLRKHEGLERDLAALGDKIKQLDETANRLMQSHPDTAEQTYTKQKEINEEWTQLTAKADSRKKKLLDSYDLQRYLSDYRDLMAWINSMMGLVASEELANDVTGAEALLERHQEHRMEIDARVGTFQAFELFGQQLLQSNHYASVEIQEKLESMNEARQELEKAWIQRRMQLDQNLELQLFCRDCEQAENWMSAREAFLSSSSADALDSGDNVEALIKKHEDFDKAINGHEEKIAALQTLADQLIAAEHYAARPIDERRCQVLDRWRHLKDALIEKRSRLGESQTLQQFSRDADEMENWIAEKLQLATEENYKDPANIQSKHQKHQAFEAELAANAERISSVLAMGGNLIEKHQCAGSEDAVQKRLASIADQWEYLTHKTTEKSMKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADINAHEERIKDMNAQADSLIESGQFEAAGIQEKRQSINERYERVRNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKLLEQAWSELKQLAATRGQKLDESLAYQQFLAKVEEEEAWIAEKQQLLSLEDYGDTMAAVQGLLKKHDAFETDFAAHGERCREIREAGEGLIAAGNHRAEAIEQRCQQLGAKLEQLGALAARRKTRLNDNSAYLQFMWKADVVESWIADKETHVRSEEFGRDLSSVQTLLTKQETFDAGLHAFEHEGIQNITSLKEMLIEAGHEQSPAIHKRHADVIARWQKLLADSQARKQRLLQMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALREAHAQFQASLSSAEADFQALGALDRQIKNFNVGPNPYTWFTMEALEDTWRNLQKIIKERDVELAKEAQRQEENDKLRKEFAKHANAFHQWLADTRTSMMEGSGSLEQQLEATKRKTQEVRARRQDLKKIEDLGAILEEHLILDNRYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNQSGVSEGALKEFSMMFKHYDKDKSGRLNHQEFKSCLRALGYDLPMVEEGQPDPEFENILDIVDPNRDGFVSLQDHMAFMISKETENVQSSQEIENAFRAITAADRPYVTKEELYANLTKEMADYCVARMKPYVDPKTERPITGALDYIEFTHTLFQN

Target ORF information:

RefSeq Version	XM_031922932.1
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031922932.1

ORF Insert Sequence:

ATGGATCAGC AGATCACCCC CAAGGAGGTG AAAATCCTCG AGAACCCAGA GGACATCCAG 
GAGAGGAGAG AGCAGGTTCT GGGACGTTAT GCCAATTTTA AGTCGGAGGC GCGCAGCAAG 
CGCGACAAAC TCGAGGACTC TCGTCGTTTC CAGTACTTTA AACGAGATGC AGATGAACTC 
GAGGGTTGGA TTTATGAGAA GTTGCAAGCT GCTTCCGATG AAAGCTACAA AGATCCGACC 
AACCTACAGG CAAAGATTCA AAAGCACCAG GCTTTTGAAG CCGAAGTCGC TGCTCATTCG 
AATGCGATTG TTCTATTAGA CAATACTGGA TCGGAAATGA TTGCTCAGCA CCACTTTGCT 
AGTGACATCA TTCGTAAAAG GCTTGAGGAA TTGCATCGCT TATGGGAATT GCTTCTTAAT 
CGACTGGCTG ACAAGGGATT GAAGCTGCAG CAGGCTTTGG TTCTTGTACA ATTCATTCGC 
CATTGCGATG AAGTCATGTT TTGGATACAT GACAAGGAAG TCTTCGTCAC CACTGATGAG 
TTTGGACAAG ATTTGGAGCA CGTAGAAGTA CTCCAAAGGA AATTTGACGA ATTCCAGAAA 
GACATGACCA GCCAAGAATA TCGTGTAACT GAAGTTAACG AATTAGCCGA TAAATTACTT 
TTGGACGCTC ATCCTGAGCG CGATACTATT TTAAGACGCA AAGAAGAATT GAATGAATCA 
TGGCAGCGAT TGAAACAACT CGCTATTCTG CGTCAAGAAA AATTGTTCGG TGCTCACGAA 
ATTCAGAGAT TCAATCGTGA TGCCGACGAG ACAATGGCTT GGATTGGAGA GAAAGACGCA 
GTACTGTCTT CCGATGATTT TGGTCGCGAT TTGGCTAGTG TACAGCTGCT CCAGCGTACT 
CACGAGAACA TTGAACGCGA TTTGGCTGCG CTCGAAGACA AGGTGGCTAC GCTTAGCACT 
GAAGCTGACA GACTAGCCGG CATCCATCAA GCCGAGCACT CTCAGCAAAT TCAGTCCAAG 
AGAGCAGAGA TTCTACAGTC CTGGGAATCG CTTACTGCTA AGGCAAAAGA ACGCCGTTTC 
AAGCTCGACG AGTCTTACTA CTTACATCGC TTCCTTGCTG ACTACCGCGA CCTTGTTTCA 
TGGATGAACA ACATGCGAGC AATCATCTCA GCTGATGAGC TTGCAAAGGA CGTGGCTGGA 
GCTGAGGCGC TTCTTGAACG TCACCAAGAG CACAAGGGCG AGATCGATGC TCGTGCCGAC 
AGCTTTGATT CAACCGGCCT CGCCGGAAGT AAGCTTCTCG AACGTCAACA CTACGCTGCG 
TCGGAGGTAG CCATCAAGCT CGAATCTCTC GCTGCTGACA AGGCTTCGTT GCTTGAACTT 
TGGGAAAAGC GACGCATTCT TTACGAGCAG TGCATGGATC TGCAGCTCTT TTACCGTGAC 
ACTGAACAAG CTGATGCCTG GATGGCCAAG CAGGAAGCTT TCCTCGCCAA CGAGGATCTT 
GGAGATTCCC TTGACAGCGT CGAGGCTCTC ATCAAGAAGC ACGAGGACTT TGACAAGTCC 
CTAGCTGCCC AGGAGGAAAA GATCAAGGCG CTTGACGAGT TTGCCAGCAA GCTCATCGAA 
GGCGAACATT ATGCGGCCGA TGAAGTAGCC CAGCGCCGGC AGGCTCTTTT GCAACGTCGC 
TCAGCCCTCC TTGAAAAGTC GGCTTTGCGC CGTCGTGCCC TTGAGGACGC CTACAAGCTG 
CAGCAGTTCG AGCGTGACTG CGATGAGACC AAAGGCTGGG TCAACGAGAA GCTTAAGTTC 
GCCACCGATG ACAGCTACCT CGACCCTACC AATCTCAATG GTAAGCTGCA GAAACACCAG 
AATTTCGAGC AGGAGCTTCA AGCAAACAAA ACGCGCATGC AAGAAATGGT CGCTCAGGGC 
CAGGAACTCA TCGAAGCCGA GCATTACGCT CGCGAGCGCA TCCAGTCGCG CATCAAGGAG 
ATCGTCGAGC TCTGGGAAAG TCTGACCGAA GCCACTGAGA AGAAGGGCAA CAAGCTTCAG 
GAAGCTGCGC AACAACAGCA ATTCAACCGC ACTGTCGAGG ACGTCGAACT CTGGCTCTCA 
GAGGTCGAAG GTCAGCTCTT GTCCGAAGAC TATGGCAAGG ACCTTACCAG CGTGCAGAAC 
CTTCAGAAAA AGCACGCCCT GCTCGAGGCC GACGTCGCCT CCCATCAAGA GCGTATCGCC 
TCTATTGCCC AGGCAGCCGA GCAGTTCGTC GCTGGAGGTC ACTTTGACGC CGACAACATC 
CGTCAGAAGC AGCAACAACT GCAGGGACGC TATTCTGCTT TGGAGCAGCC TATGCAGATG 
CGCAAGCAGC GCTTGCTGGA CTCGCTGCAG GTACAGCAGC TGTTCCGCGA TGTCGAAGAC 
GAGGAGGCCT GGATCCGCGA GAAGGAGCCT GTGGCAGCCA GCACCAACCG TGGTCGCGAT 
CTCATTGGTG TGCAGAACCT GCAAAAGAAG CACCAGGCCG TCCTTGCTGA AATCAACAAT 
CATGAGCCCC GAGTTGCAGC CGTCTGCGCA GCTGGCTCAC GCATGTTGAG CGATGGTCAT 
TTCGCAGCTG AAGAGATCAG CCAGAGACTG GCTAGTCTCG ATGAACATTG GGGACAGTTG 
AAGGAGAAGG CTCGCCAGCG TAAGAACGAC CTTGATGACT CGCTCCAGGC TCACCAATAC 
TTCGCCGATG CCAATGAAGC CGAGTCTTGG ATGAAGGAGA AGCGCCCCAT CGTCATGAAC 
GCCGATTACG GCAAAGACGA GGATAGTAGC GAGGCTCTGC TCAAGAAACA CGAGGCTCTC 
GTGAGCGATC TCGAAGCTTT TGCTAGCACC ATTGCAGCGC TCAAGGAACA GGCAGCCGCC 
TGCCGTCAGC AAGAGACACC TGCTGTCGAC GTAGCTGGAA AGGAATGCGT TGTCGCACTC 
TACGATTACA CCGAGAAGTC TCCTCGCGAG GTCTCTATGA AGAAAGGAGA CACCCTTACA 
CTCCTCAACA GCAGCAACAA GGACTGGTGG AAGGTGGAGG TAAACGATCG TCAGGGCTTC 
GTGCCAGCCG CCTATGTCAA GCGCGTAGAA CCGGACGCGG GTCTTAGTGC TTCTCAGCAG 
AATCTGGCTC GCGAGCAGGG CTCCATTGCC GCTCGCCAGG CCCAGATTGA AGCCCAATAC 
GAAGACTTGC TGCGATTGGC CAGAGAAAGA CAAAACAAGC TTAATGAAAC CGCCAAGGCG 
TACGTGTTGG TTCGCGAGGC CGCTGAACTG GCCACCTGGA TCAAGGGCAA GGAGAACCAT 
GCTCAGGTAC AGGATGTAGG CGAGGATTTG GAACAAGTCG AGGTTATGCA GAAGAAGTTC 
GACGATTTCC GAGCTGATCT GAAGGCAAAC GAGGTGCGTC TCGCAGAGAT GAACGAGATC 
GCCGTGCAGC TGATGAGCGT CGGCCAGACC GAGGCAGCTC TTAAGATCCA GACGCAGATC 
CAAGAGCTTA ACGAAAAATG GACGAGTCTG CAGACTCTAA CAGCCGAGCG CGCCAGTCAG 
CTGGACTCTG CTCACGAAGT ACAGCGCTTC CATCGCGATG TCGACGACAC CAAAGACTGG 
ATTCGCGAGA AGGATGCTGC CCTGAACAAC GATGACCTGG GCAAGGACTT GCGCAGCGTG 
CAAACTCTGC TCCGCAAGCA CGAAGGTCTG GAGCGCGACC TCGCTGCCCT TGGGGACAAG 
ATTAAGCAGT TGGACGAAAC AGCAAACCGC TTGATGCAGT CGCATCCTGA TACGGCCGAG 
CAAACCTACA CCAAGCAGAA GGAGATCAAC GAGGAATGGA CTCAGCTTAC TGCCAAAGCT 
GATTCTAGAA AGAAGAAGCT GCTCGATTCT TATGACCTGC AACGCTATCT CAGCGACTAT 
CGGGATCTTA TGGCCTGGAT TAATTCGATG ATGGGTCTGG TAGCTTCCGA AGAATTGGCT 
AACGACGTCA CCGGTGCTGA GGCTCTGCTT GAACGTCACC AGGAACATCG CATGGAAATC 
GACGCCCGCG TTGGAACTTT CCAAGCTTTC GAGCTCTTCG GCCAGCAACT CTTGCAATCC 
AACCACTATG CTAGCGTTGA GATACAAGAG AAACTCGAAT CTATGAACGA GGCTCGTCAA 
GAACTTGAGA AGGCTTGGAT ACAGCGCCGT ATGCAGTTGG ACCAGAACCT CGAGCTGCAA 
CTCTTCTGCC GCGATTGCGA ACAGGCCGAG AACTGGATGT CCGCTCGTGA AGCTTTCCTT 
AGTAGCAGTA GCGCTGATGC CCTCGACAGC GGTGACAATG TAGAGGCCCT CATCAAGAAG 
CACGAGGACT TCGACAAGGC TATCAACGGT CACGAAGAGA AAATAGCCGC TCTTCAAACT 
CTGGCTGATC AGCTTATTGC TGCTGAGCAC TACGCTGCTC GACCTATCGA CGAACGTCGC 
TGCCAGGTAC TCGATCGCTG GCGTCACTTG AAAGATGCTC TCATTGAGAA ACGATCACGG 
CTTGGCGAGT CCCAAACTCT GCAGCAATTC TCTCGCGATG CTGATGAGAT GGAGAATTGG 
ATCGCTGAGA AGCTGCAGCT CGCCACTGAA GAGAACTACA AAGATCCGGC TAATATACAG 
TCCAAACATC AAAAACACCA AGCCTTTGAA GCTGAGCTTG CTGCAAATGC CGAACGTATC 
TCGTCGGTGC TGGCTATGGG TGGTAACCTG ATCGAAAAGC ATCAGTGCGC TGGCTCTGAA 
GATGCCGTGC AGAAGCGTCT TGCCTCTATT GCCGATCAGT GGGAGTACCT CACTCACAAA 
ACAACCGAAA AATCCATGAA ACTAAAGGAA GCCAATAAGC AACGCACCTA CATCGCTGCA 
GTCAAGGATT TAGACTTTTG GCTCGGTGAG GTCGAGAGTC TGCTGACAAG CGAAGATGCT 
GGCAAGGATC TCGCCTCAGT GCAGAACCTC ATGAAGAAGC ATCAACTGGT TGAGGCCGAC 
ATCAACGCCC ACGAGGAGCG TATTAAAGAC ATGAATGCTC AGGCTGACTC CCTCATCGAG 
AGCGGTCAAT TCGAGGCAGC GGGTATCCAA GAAAAGCGTC AAAGCATCAA CGAACGCTAT 
GAACGCGTAC GCAACCTCGC AGCTCACAGG CAGGCTAGAC TCAATGAGGC TAACACCTTG 
CATCAGTTCT TCCGCGACAT CGCCGACGAG GAGTCCTGGA TCAAGGAGAA GAAGCTTCTC 
GTTGGTTCAG ATGACTACGG TCGTGACCTT ACAGGCGTTC AGAATTTAAA GAAAAAGCAC 
AAGCGTCTCG AGGCTGAGCT TGGCAGCCAC GAGCCCGCTA TCCAAGCCGT ACAAGAAGCT 
GGTGAGAAAC TCATGGACGT CTCCAACCTA GGCGTACCAG AGATCGAGCA ACGACTCAAG 
CTTCTCGAAC AGGCGTGGTC TGAATTGAAA CAACTAGCTG CCACGCGTGG TCAGAAGCTT 
GACGAGTCGC TGGCTTACCA GCAATTTTTG GCAAAGGTTG AAGAGGAAGA AGCCTGGATC 
GCCGAGAAAC AGCAATTGCT CTCACTCGAG GATTACGGCG ATACAATGGC TGCCGTGCAA 
GGTTTGCTCA AGAAGCACGA CGCTTTCGAG ACAGACTTTG CTGCCCACGG TGAACGTTGC 
CGTGAAATCC GCGAAGCCGG TGAGGGACTC ATAGCTGCAG GTAACCATCG TGCTGAGGCA 
ATCGAACAGC GCTGCCAGCA GCTAGGAGCC AAGCTTGAAC AGCTTGGAGC TTTGGCTGCT 
AGACGCAAGA CTCGCCTCAA CGATAACTCA GCTTACCTGC AGTTCATGTG GAAGGCTGAC 
GTAGTCGAGT CCTGGATCGC TGACAAGGAA ACACACGTAC GATCCGAAGA GTTTGGCCGT 
GATTTGTCCA GTGTGCAGAC TTTGCTCACC AAACAGGAGA CCTTCGACGC TGGTCTGCAC 
GCTTTCGAAC ACGAGGGTAT TCAGAACATA ACTTCTCTCA AGGAGATGCT AATCGAGGCC 
GGTCATGAAC AGAGCCCCGC GATTCACAAA CGTCACGCTG ACGTTATCGC TCGCTGGCAG 
AAGCTGCTTG CCGATTCGCA AGCCCGTAAG CAGCGTCTCT TGCAGATGCA GGAGCAATTC 
CGTCAGATAG AAGAGCTTTA CCTGACTTTC GCCAAGAAGG CATCGGCCTT CAACTCGTGG 
TTCGAAAACG CTGAGGAGGA TTTGACCGAC CCTGTCCGCT GCAACAGCAT CGAAGAGATC 
CGTGCTTTGC GCGAAGCTCA TGCGCAGTTC CAAGCTAGTC TGTCATCGGC TGAGGCCGAC 
TTCCAGGCTC TTGGAGCTCT TGACCGCCAA ATCAAGAACT TCAATGTTGG CCCCAACCCT 
TACACCTGGT TCACCATGGA AGCGCTCGAG GACACCTGGC GCAACTTGCA GAAGATCATC 
AAGGAGCGCG ACGTCGAATT AGCTAAGGAA GCTCAGCGTC AAGAGGAAAA CGACAAGTTG 
CGAAAGGAGT TCGCCAAGCA CGCCAATGCC TTCCATCAGT GGCTCGCGGA CACCCGTACT 
TCTATGATGG AAGGATCGGG CTCACTTGAA CAACAGCTCG AAGCAACAAA GAGAAAGACA 
CAAGAAGTCC GAGCGCGGCG TCAAGATCTC AAAAAGATCG AGGATTTGGG CGCAATACTG 
GAAGAACATT TGATTCTCGA CAACAGGTAC ACAGAACACA GTACTGTAGG ACTGGCCCAA 
CAATGGGACC AGCTCGACCA GTTGGGTATG CGTATGCAGC ATAACCTGGA ACAACAGATT 
CAAGCTCGTA ACCAATCTGG AGTGTCGGAA GGAGCCCTCA AGGAGTTCTC GATGATGTTC 
AAGCATTACG ACAAGGACAA GAGCGGACGA CTAAATCACC AAGAGTTCAA GTCGTGCCTC 
AGAGCTTTGG GTTACGATCT ACCTATGGTG GAAGAAGGAC AACCAGATCC AGAATTCGAA 
AATATTCTAG ACATTGTCGA CCCGAACAGA GATGGCTTTG TTTCCTTACA AGATCACATG 
GCATTCATGA TCAGCAAGGA AACTGAGAAT GTCCAAAGTT CACAAGAAAT CGAAAACGCC 
TTCCGAGCTA TTACAGCTGC CGATCGTCCT TACGTCACGA AAGAGGAACT CTACGCTAAC 
CTCACGAAAG AAATGGCGGA CTATTGTGTG GCGAGGATGA AACCATACGT CGACCCGAAG 
ACGGAACGAC CGATCACCGG CGCACTAGAC TATATAGAAT TTACACACAC GTTATTCCAG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100116996 ( XM_016981977.1 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_016837466.1 Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X1, mRNA.

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CloneID	ONd22430
Clone ID Related Accession (Same CDS sequence)	XM_016981977.1 , XM_016981977.2
Accession Version	XM_016981977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464105600000
Organism	Nasonia vitripennis(jewel wasp)
Product	spectrin alpha chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015868.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nasonia vitripennis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGC AGATCACCCC CAAGGAGGTG AAAATCCTCG AGAACCCAGA GGACATCCAG 
GAGAGGAGAG AGCAGGTTCT GGGACGTTAT GCCAATTTTA AGTCGGAGGC GCGCAGCAAG 
CGCGACAAAC TCGAGGACTC TCGTCGTTTC CAGTACTTTA AACGAGATGC AGATGAACTC 
GAGGGTTGGA TTTATGAGAA GTTGCAAGCT GCTTCCGATG AAAGCTACAA AGATCCGACC 
AACCTACAGG CAAAGATTCA AAAGCACCAG GCTTTTGAAG CCGAAGTCGC TGCTCATTCG 
AATGCGATTG TTCTATTAGA CAATACTGGA TCGGAAATGA TTGCTCAGCA CCACTTTGCT 
AGTGACATCA TTCGTAAAAG GCTTGAGGAA TTGCATCGCT TATGGGAATT GCTTCTTAAT 
CGACTGGCTG ACAAGGGATT GAAGCTGCAG CAGGCTTTGG TTCTTGTACA ATTCATTCGC 
CATTGCGATG AAGTCATGTT TTGGATACAT GACAAGGAAG TCTTCGTCAC CACTGATGAG 
TTTGGACAAG ATTTGGAGCA CGTAGAAGTA CTCCAAAGGA AATTTGACGA ATTCCAGAAA 
GACATGACCA GCCAAGAATA TCGTGTAACT GAAGTTAACG AATTAGCCGA TAAATTACTT 
TTGGACGCTC ATCCTGAGCG CGATACTATT TTAAGACGCA AAGAAGAATT GAATGAATCA 
TGGCAGCGAT TGAAACAACT CGCTATTCTG CGTCAAGAAA AATTGTTCGG TGCTCACGAA 
ATTCAGAGAT TCAATCGTGA TGCCGACGAG ACAATGGCTT GGATTGGAGA GAAAGACGCA 
GTACTGTCTT CCGATGATTT TGGTCGCGAT TTGGCTAGTG TACAGCTGCT CCAGCGTACT 
CACGAGAACA TTGAACGCGA TTTGGCTGCG CTCGAAGACA AGGTGGCTAC GCTTAGCACT 
GAAGCTGACA GACTAGCCGG CATCCATCAA GCCGAGCACT CTCAGCAAAT TCAGTCCAAG 
AGAGCAGAGA TTCTACAGTC CTGGGAATCG CTTACTGCTA AGGCAAAAGA ACGCCGTTTC 
AAGCTCGACG AGTCTTACTA CTTACATCGC TTCCTTGCTG ACTACCGCGA CCTTGTTTCA 
TGGATGAACA ACATGCGAGC AATCATCTCA GCTGATGAGC TTGCAAAGGA CGTGGCTGGA 
GCTGAGGCGC TTCTTGAACG TCACCAAGAG CACAAGGGCG AGATCGATGC TCGTGCCGAC 
AGCTTTGATT CAACCGGCCT CGCCGGAAGT AAGCTTCTCG AACGTCAACA CTACGCTGCG 
TCGGAGGTAG CCATCAAGCT CGAATCTCTC GCTGCTGACA AGGCTTCGTT GCTTGAACTT 
TGGGAAAAGC GACGCATTCT TTACGAGCAG TGCATGGATC TGCAGCTCTT TTACCGTGAC 
ACTGAACAAG CTGATGCCTG GATGGCCAAG CAGGAAGCTT TCCTCGCCAA CGAGGATCTT 
GGAGATTCCC TTGACAGCGT CGAGGCTCTC ATCAAGAAGC ACGAGGACTT TGACAAGTCC 
CTAGCTGCCC AGGAGGAAAA GATCAAGGCG CTTGACGAGT TTGCCAGCAA GCTCATCGAA 
GGCGAACATT ATGCGGCCGA TGAAGTAGCC CAGCGCCGGC AGGCTCTTTT GCAACGTCGC 
TCAGCCCTCC TTGAAAAGTC GGCTTTGCGC CGTCGTGCCC TTGAGGACGC CTACAAGCTG 
CAGCAGTTCG AGCGTGACTG CGATGAGACC AAAGGCTGGG TCAACGAGAA GCTTAAGTTC 
GCCACCGATG ACAGCTACCT CGACCCTACC AATCTCAATG GTAAGCTGCA GAAACACCAG 
AATTTCGAGC AGGAGCTTCA AGCAAACAAA ACGCGCATGC AAGAAATGGT CGCTCAGGGC 
CAGGAACTCA TCGAAGCCGA GCATTACGCT CGCGAGCGCA TCCAGTCGCG CATCAAGGAG 
ATCGTCGAGC TCTGGGAAAG TCTGACCGAA GCCACTGAGA AGAAGGGCAA CAAGCTTCAG 
GAAGCTGCGC AACAACAGCA ATTCAACCGC ACTGTCGAGG ACGTCGAACT CTGGCTCTCA 
GAGGTCGAAG GTCAGCTCTT GTCCGAAGAC TATGGCAAGG ACCTTACCAG CGTGCAGAAC 
CTTCAGAAAA AGCACGCCCT GCTCGAGGCC GACGTCGCCT CCCATCAAGA GCGTATCGCC 
TCTATTGCCC AGGCAGCCGA GCAGTTCGTC GCTGGAGGTC ACTTTGACGC CGACAACATC 
CGTCAGAAGC AGCAACAACT GCAGGGACGC TATTCTGCTT TGGAGCAGCC TATGCAGATG 
CGCAAGCAGC GCTTGCTGGA CTCGCTGCAG GTACAGCAGC TGTTCCGCGA TGTCGAAGAC 
GAGGAGGCCT GGATCCGCGA GAAGGAGCCT GTGGCAGCCA GCACCAACCG TGGTCGCGAT 
CTCATTGGTG TGCAGAACCT GCAAAAGAAG CACCAGGCCG TCCTTGCTGA AATCAACAAT 
CATGAGCCCC GAGTTGCAGC CGTCTGCGCA GCTGGCTCAC GCATGTTGAG CGATGGTCAT 
TTCGCAGCTG AAGAGATCAG CCAGAGACTG GCTAGTCTCG ATGAACATTG GGGACAGTTG 
AAGGAGAAGG CTCGCCAGCG TAAGAACGAC CTTGATGACT CGCTCCAGGC TCACCAATAC 
TTCGCCGATG CCAATGAAGC CGAGTCTTGG ATGAAGGAGA AGCGCCCCAT CGTCATGAAC 
GCCGATTACG GCAAAGACGA GGATAGTAGC GAGGCTCTGC TCAAGAAACA CGAGGCTCTC 
GTGAGCGATC TCGAAGCTTT TGCTAGCACC ATTGCAGCGC TCAAGGAACA GGCAGCCGCC 
TGCCGTCAGC AAGAGACACC TGCTGTCGAC GTAGCTGGAA AGGAATGCGT TGTCGCACTC 
TACGATTACA CCGAGAAGTC TCCTCGCGAG GTCTCTATGA AGAAAGGAGA CACCCTTACA 
CTCCTCAACA GCAGCAACAA GGACTGGTGG AAGGTGGAGG TAAACGATCG TCAGGGCTTC 
GTGCCAGCCG CCTATGTCAA GCGCGTAGAA CCGGACGCGG GTCTTAGTGC TTCTCAGCAG 
AATCTGGCTC GCGAGCAGGG CTCCATTGCC GCTCGCCAGG CCCAGATTGA AGCCCAATAC 
GAAGACTTGC TGCGATTGGC CAGAGAAAGA CAAAACAAGC TTAATGAAAC CGCCAAGGCG 
TACGTGTTGG TTCGCGAGGC CGCTGAACTG GCCACCTGGA TCAAGGGCAA GGAGAACCAT 
GCTCAGGTAC AGGATGTAGG CGAGGATTTG GAACAAGTCG AGGTTATGCA GAAGAAGTTC 
GACGATTTCC GAGCTGATCT GAAGGCAAAC GAGGTGCGTC TCGCAGAGAT GAACGAGATC 
GCCGTGCAGC TGATGAGCGT CGGCCAGACC GAGGCAGCTC TTAAGATCCA GACGCAGATC 
CAAGAGCTTA ACGAAAAATG GACGAGTCTG CAGACTCTAA CAGCCGAGCG CGCCAGTCAG 
CTGGACTCTG CTCACGAAGT ACAGCGCTTC CATCGCGATG TCGACGACAC CAAAGACTGG 
ATTCGCGAGA AGGATGCTGC CCTGAACAAC GATGACCTGG GCAAGGACTT GCGCAGCGTG 
CAAACTCTGC TCCGCAAGCA CGAAGGTCTG GAGCGCGACC TCGCTGCCCT TGGGGACAAG 
ATTAAGCAGT TGGACGAAAC AGCAAACCGC TTGATGCAGT CGCATCCTGA TACGGCCGAG 
CAAACCTACA CCAAGCAGAA GGAGATCAAC GAGGAATGGA CTCAGCTTAC TGCCAAAGCT 
GATTCTAGAA AGAAGAAGCT GCTCGATTCT TATGACCTGC AACGCTATCT CAGCGACTAT 
CGGGATCTTA TGGCCTGGAT TAATTCGATG ATGGGTCTGG TAGCTTCCGA AGAATTGGCT 
AACGACGTCA CCGGTGCTGA GGCTCTGCTT GAACGTCACC AGAATCACAG AACAGAAATC 
GATGCACGAT ATGGCATTGT TCCACAGGAA CATCGCATGG AAATCGACGC CCGCGTTGGA 
ACTTTCCAAG CTTTCGAGCT CTTCGGCCAG CAACTCTTGC AATCCAACCA CTATGCTAGC 
GTTGAGATAC AAGAGAAACT CGAATCTATG AACGAGGCTC GTCAAGAACT TGAGAAGGCT 
TGGATACAGC GCCGTATGCA GTTGGACCAG AACCTCGAGC TGCAACTCTT CTGCCGCGAT 
TGCGAACAGG CCGAGAACTG GATGTCCGCT CGTGAAGCTT TCCTTAGTAG CAGTAGCGCT 
GATGCCCTCG ACAGCGGTGA CAATGTAGAG GCCCTCATCA AGAAGCACGA GGACTTCGAC 
AAGGCTATCA ACGGTCACGA AGAGAAAATA GCCGCTCTTC AAACTCTGGC TGATCAGCTT 
ATTGCTGCTG AGCACTACGC TGCTCGACCT ATCGACGAAC GTCGCTGCCA GGTACTCGAT 
CGCTGGCGTC ACTTGAAAGA TGCTCTCATT GAGAAACGAT CACGGCTTGG CGAGTCCCAA 
ACTCTGCAGC AATTCTCTCG CGATGCTGAT GAGATGGAGA ATTGGATCGC TGAGAAGCTG 
CAGCTCGCCA CTGAAGAGAA CTACAAAGAT CCGGCTAATA TACAGTCCAA ACATCAAAAA 
CACCAAGCCT TTGAAGCTGA GCTTGCTGCA AATGCCGAAC GTATCTCGTC GGTGCTGGCT 
ATGGGTGGTA ACCTGATCGA AAAGCATCAG TGCGCTGGCT CTGAAGATGC CGTGCAGAAG 
CGTCTTGCCT CTATTGCCGA TCAGTGGGAG TACCTCACTC ACAAAACAAC CGAAAAATCC 
ATGAAACTAA AGGAAGCCAA TAAGCAACGC ACCTACATCG CTGCAGTCAA GGATTTAGAC 
TTTTGGCTCG GTGAGGTCGA GAGTCTGCTG ACAAGCGAAG ATGCTGGCAA GGATCTCGCC 
TCAGTGCAGA ACCTCATGAA GAAGCATCAA CTGGTTGAGG CCGACATCAA CGCCCACGAG 
GAGCGTATTA AAGACATGAA TGCTCAGGCT GACTCCCTCA TCGAGAGCGG TCAATTCGAG 
GCAGCGGGTA TCCAAGAAAA GCGTCAAAGC ATCAACGAAC GCTATGAACG CGTACGCAAC 
CTCGCAGCTC ACAGGCAGGC TAGACTCAAT GAGGCTAACA CCTTGCATCA GTTCTTCCGC 
GACATCGCCG ACGAGGAGTC CTGGATCAAG GAGAAGAAGC TTCTCGTTGG TTCAGATGAC 
TACGGTCGTG ACCTTACAGG CGTTCAGAAT TTAAAGAAAA AGCACAAGCG TCTCGAGGCT 
GAGCTTGGCA GCCACGAGCC CGCTATCCAA GCCGTACAAG AAGCTGGTGA GAAACTCATG 
GACGTCTCCA ACCTAGGCGT ACCAGAGATC GAGCAACGAC TCAAGCTTCT CGAACAGGCG 
TGGTCTGAAT TGAAACAACT AGCTGCCACG CGTGGTCAGA AGCTTGACGA GTCGCTGGCT 
TACCAGCAAT TTTTGGCAAA GGTTGAAGAG GAAGAAGCCT GGATCGCCGA GAAACAGCAA 
TTGCTCTCAC TCGAGGATTA CGGCGATACA ATGGCTGCCG TGCAAGGTTT GCTCAAGAAG 
CACGACGCTT TCGAGACAGA CTTTGCTGCC CACGGTGAAC GTTGCCGTGA AATCCGCGAA 
GCCGGTGAGG GACTCATAGC TGCAGGTAAC CATCGTGCTG AGGCAATCGA ACAGCGCTGC 
CAGCAGCTAG GAGCCAAGCT TGAACAGCTT GGAGCTTTGG CTGCTAGACG CAAGACTCGC 
CTCAACGATA ACTCAGCTTA CCTGCAGTTC ATGTGGAAGG CTGACGTAGT CGAGTCCTGG 
ATCGCTGACA AGGAAACACA CGTACGATCC GAAGAGTTTG GCCGTGATTT GTCCAGTGTG 
CAGACTTTGC TCACCAAACA GGAGACCTTC GACGCTGGTC TGCACGCTTT CGAACACGAG 
GGTATTCAGA ACATAACTTC TCTCAAGGAG ATGCTAATCG AGGCCGGTCA TGAACAGAGC 
CCCGCGATTC ACAAACGTCA CGCTGACGTT ATCGCTCGCT GGCAGAAGCT GCTTGCCGAT 
TCGCAAGCCC GTAAGCAGCG TCTCTTGCAG ATGCAGGAGC AATTCCGTCA GATAGAAGAG 
CTTTACCTGA CTTTCGCCAA GAAGGCATCG GCCTTCAACT CGTGGTTCGA AAACGCTGAG 
GAGGATTTGA CCGACCCTGT CCGCTGCAAC AGCATCGAAG AGATCCGTGC TTTGCGCGAA 
GCTCATGCGC AGTTCCAAGC TAGTCTGTCA TCGGCTGAGG CCGACTTCCA GGCTCTTGGA 
GCTCTTGACC GCCAAATCAA GAACTTCAAT GTTGGCCCCA ACCCTTACAC CTGGTTCACC 
ATGGAAGCGC TCGAGGACAC CTGGCGCAAC TTGCAGAAGA TCATCAAGGA GCGCGACGTC 
GAATTAGCTA AGGAAGCTCA GCGTCAAGAG GAAAACGACA AGTTGCGAAA GGAGTTCGCC 
AAGCACGCCA ATGCCTTCCA TCAGTGGCTC GCGGACACCC GTACTTCTAT GATGGAAGGA 
TCGGGCTCAC TTGAACAACA GCTCGAAGCA ACAAAGAGAA AGACACAAGA AGTCCGAGCG 
CGGCGTCAAG ATCTCAAAAA GATCGAGGAT TTGGGCGCAA TACTGGAAGA ACATTTGATT 
CTCGACAACA GGTACACAGA ACACAGTACT GTAGGACTGG CCCAACAATG GGACCAGCTC 
GACCAGTTGG GTATGCGTAT GCAGCATAAC CTGGAACAAC AGATTCAAGC TCGTAACCAA 
TCTGGAGTGT CGGAAGGAGC CCTCAAGGAG TTCTCGATGA TGTTCAAGCA TTACGACAAG 
GACAAGAGCG GACGACTAAA TCACCAAGAG TTCAAGTCGT GCCTCAGAGC TTTGGGTTAC 
GATCTACCTA TGGTGGAAGA AGGACAACCA GATCCAGAAT TCGAAAATAT TCTAGACATT 
GTCGACCCGA ACAGAGATGG CTTTGTTTCC TTACAAGATC ACATGGCATT CATGATCAGC 
AAGGAAACTG AGAATGTCCA AAGTTCACAA GAAATCGAAA ACGCCTTCCG AGCTATTACA 
GCTGCCGATC GTCCTTACGT CACGAAAGAG GAACTCTACG CTAACCTCAC GAAAGAAATG 
GCGGACTATT GTGTGGCGAG GATGAAACCA TACGTCGACC CGAAGACGGA ACGACCGATC 
ACCGGCGCAC TAGACTATAT AGAATTTACA CACACGTTAT TCCAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016837466.1
CDS	453..7763
Translation	MDQQITPKEVKILENPEDIQERREQVLGRYANFKSEARSKRDKLEDSRRFQYFKRDADELEGWIYEKLQAASDESYKDPTNLQAKIQKHQAFEAEVAAHSNAIVLLDNTGSEMIAQHHFASDIIRKRLEELHRLWELLLNRLADKGLKLQQALVLVQFIRHCDEVMFWIHDKEVFVTTDEFGQDLEHVEVLQRKFDEFQKDMTSQEYRVTEVNELADKLLLDAHPERDTILRRKEELNESWQRLKQLAILRQEKLFGAHEIQRFNRDADETMAWIGEKDAVLSSDDFGRDLASVQLLQRTHENIERDLAALEDKVATLSTEADRLAGIHQAEHSQQIQSKRAEILQSWESLTAKAKERRFKLDESYYLHRFLADYRDLVSWMNNMRAIISADELAKDVAGAEALLERHQEHKGEIDARADSFDSTGLAGSKLLERQHYAASEVAIKLESLAADKASLLELWEKRRILYEQCMDLQLFYRDTEQADAWMAKQEAFLANEDLGDSLDSVEALIKKHEDFDKSLAAQEEKIKALDEFASKLIEGEHYAADEVAQRRQALLQRRSALLEKSALRRRALEDAYKLQQFERDCDETKGWVNEKLKFATDDSYLDPTNLNGKLQKHQNFEQELQANKTRMQEMVAQGQELIEAEHYARERIQSRIKEIVELWESLTEATEKKGNKLQEAAQQQQFNRTVEDVELWLSEVEGQLLSEDYGKDLTSVQNLQKKHALLEADVASHQERIASIAQAAEQFVAGGHFDADNIRQKQQQLQGRYSALEQPMQMRKQRLLDSLQVQQLFRDVEDEEAWIREKEPVAASTNRGRDLIGVQNLQKKHQAVLAEINNHEPRVAAVCAAGSRMLSDGHFAAEEISQRLASLDEHWGQLKEKARQRKNDLDDSLQAHQYFADANEAESWMKEKRPIVMNADYGKDEDSSEALLKKHEALVSDLEAFASTIAALKEQAAACRQQETPAVDVAGKECVVALYDYTEKSPREVSMKKGDTLTLLNSSNKDWWKVEVNDRQGFVPAAYVKRVEPDAGLSASQQNLAREQGSIAARQAQIEAQYEDLLRLARERQNKLNETAKAYVLVREAAELATWIKGKENHAQVQDVGEDLEQVEVMQKKFDDFRADLKANEVRLAEMNEIAVQLMSVGQTEAALKIQTQIQELNEKWTSLQTLTAERASQLDSAHEVQRFHRDVDDTKDWIREKDAALNNDDLGKDLRSVQTLLRKHEGLERDLAALGDKIKQLDETANRLMQSHPDTAEQTYTKQKEINEEWTQLTAKADSRKKKLLDSYDLQRYLSDYRDLMAWINSMMGLVASEELANDVTGAEALLERHQNHRTEIDARYGIVPQEHRMEIDARVGTFQAFELFGQQLLQSNHYASVEIQEKLESMNEARQELEKAWIQRRMQLDQNLELQLFCRDCEQAENWMSAREAFLSSSSADALDSGDNVEALIKKHEDFDKAINGHEEKIAALQTLADQLIAAEHYAARPIDERRCQVLDRWRHLKDALIEKRSRLGESQTLQQFSRDADEMENWIAEKLQLATEENYKDPANIQSKHQKHQAFEAELAANAERISSVLAMGGNLIEKHQCAGSEDAVQKRLASIADQWEYLTHKTTEKSMKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADINAHEERIKDMNAQADSLIESGQFEAAGIQEKRQSINERYERVRNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKLLEQAWSELKQLAATRGQKLDESLAYQQFLAKVEEEEAWIAEKQQLLSLEDYGDTMAAVQGLLKKHDAFETDFAAHGERCREIREAGEGLIAAGNHRAEAIEQRCQQLGAKLEQLGALAARRKTRLNDNSAYLQFMWKADVVESWIADKETHVRSEEFGRDLSSVQTLLTKQETFDAGLHAFEHEGIQNITSLKEMLIEAGHEQSPAIHKRHADVIARWQKLLADSQARKQRLLQMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALREAHAQFQASLSSAEADFQALGALDRQIKNFNVGPNPYTWFTMEALEDTWRNLQKIIKERDVELAKEAQRQEENDKLRKEFAKHANAFHQWLADTRTSMMEGSGSLEQQLEATKRKTQEVRARRQDLKKIEDLGAILEEHLILDNRYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNQSGVSEGALKEFSMMFKHYDKDKSGRLNHQEFKSCLRALGYDLPMVEEGQPDPEFENILDIVDPNRDGFVSLQDHMAFMISKETENVQSSQEIENAFRAITAADRPYVTKEELYANLTKEMADYCVARMKPYVDPKTERPITGALDYIEFTHTLFQN

Target ORF information:

RefSeq Version	XM_016981977.1
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016981977.1

ORF Insert Sequence:

ATGGATCAGC AGATCACCCC CAAGGAGGTG AAAATCCTCG AGAACCCAGA GGACATCCAG 
GAGAGGAGAG AGCAGGTTCT GGGACGTTAT GCCAATTTTA AGTCGGAGGC GCGCAGCAAG 
CGCGACAAAC TCGAGGACTC TCGTCGTTTC CAGTACTTTA AACGAGATGC AGATGAACTC 
GAGGGTTGGA TTTATGAGAA GTTGCAAGCT GCTTCCGATG AAAGCTACAA AGATCCGACC 
AACCTACAGG CAAAGATTCA AAAGCACCAG GCTTTTGAAG CCGAAGTCGC TGCTCATTCG 
AATGCGATTG TTCTATTAGA CAATACTGGA TCGGAAATGA TTGCTCAGCA CCACTTTGCT 
AGTGACATCA TTCGTAAAAG GCTTGAGGAA TTGCATCGCT TATGGGAATT GCTTCTTAAT 
CGACTGGCTG ACAAGGGATT GAAGCTGCAG CAGGCTTTGG TTCTTGTACA ATTCATTCGC 
CATTGCGATG AAGTCATGTT TTGGATACAT GACAAGGAAG TCTTCGTCAC CACTGATGAG 
TTTGGACAAG ATTTGGAGCA CGTAGAAGTA CTCCAAAGGA AATTTGACGA ATTCCAGAAA 
GACATGACCA GCCAAGAATA TCGTGTAACT GAAGTTAACG AATTAGCCGA TAAATTACTT 
TTGGACGCTC ATCCTGAGCG CGATACTATT TTAAGACGCA AAGAAGAATT GAATGAATCA 
TGGCAGCGAT TGAAACAACT CGCTATTCTG CGTCAAGAAA AATTGTTCGG TGCTCACGAA 
ATTCAGAGAT TCAATCGTGA TGCCGACGAG ACAATGGCTT GGATTGGAGA GAAAGACGCA 
GTACTGTCTT CCGATGATTT TGGTCGCGAT TTGGCTAGTG TACAGCTGCT CCAGCGTACT 
CACGAGAACA TTGAACGCGA TTTGGCTGCG CTCGAAGACA AGGTGGCTAC GCTTAGCACT 
GAAGCTGACA GACTAGCCGG CATCCATCAA GCCGAGCACT CTCAGCAAAT TCAGTCCAAG 
AGAGCAGAGA TTCTACAGTC CTGGGAATCG CTTACTGCTA AGGCAAAAGA ACGCCGTTTC 
AAGCTCGACG AGTCTTACTA CTTACATCGC TTCCTTGCTG ACTACCGCGA CCTTGTTTCA 
TGGATGAACA ACATGCGAGC AATCATCTCA GCTGATGAGC TTGCAAAGGA CGTGGCTGGA 
GCTGAGGCGC TTCTTGAACG TCACCAAGAG CACAAGGGCG AGATCGATGC TCGTGCCGAC 
AGCTTTGATT CAACCGGCCT CGCCGGAAGT AAGCTTCTCG AACGTCAACA CTACGCTGCG 
TCGGAGGTAG CCATCAAGCT CGAATCTCTC GCTGCTGACA AGGCTTCGTT GCTTGAACTT 
TGGGAAAAGC GACGCATTCT TTACGAGCAG TGCATGGATC TGCAGCTCTT TTACCGTGAC 
ACTGAACAAG CTGATGCCTG GATGGCCAAG CAGGAAGCTT TCCTCGCCAA CGAGGATCTT 
GGAGATTCCC TTGACAGCGT CGAGGCTCTC ATCAAGAAGC ACGAGGACTT TGACAAGTCC 
CTAGCTGCCC AGGAGGAAAA GATCAAGGCG CTTGACGAGT TTGCCAGCAA GCTCATCGAA 
GGCGAACATT ATGCGGCCGA TGAAGTAGCC CAGCGCCGGC AGGCTCTTTT GCAACGTCGC 
TCAGCCCTCC TTGAAAAGTC GGCTTTGCGC CGTCGTGCCC TTGAGGACGC CTACAAGCTG 
CAGCAGTTCG AGCGTGACTG CGATGAGACC AAAGGCTGGG TCAACGAGAA GCTTAAGTTC 
GCCACCGATG ACAGCTACCT CGACCCTACC AATCTCAATG GTAAGCTGCA GAAACACCAG 
AATTTCGAGC AGGAGCTTCA AGCAAACAAA ACGCGCATGC AAGAAATGGT CGCTCAGGGC 
CAGGAACTCA TCGAAGCCGA GCATTACGCT CGCGAGCGCA TCCAGTCGCG CATCAAGGAG 
ATCGTCGAGC TCTGGGAAAG TCTGACCGAA GCCACTGAGA AGAAGGGCAA CAAGCTTCAG 
GAAGCTGCGC AACAACAGCA ATTCAACCGC ACTGTCGAGG ACGTCGAACT CTGGCTCTCA 
GAGGTCGAAG GTCAGCTCTT GTCCGAAGAC TATGGCAAGG ACCTTACCAG CGTGCAGAAC 
CTTCAGAAAA AGCACGCCCT GCTCGAGGCC GACGTCGCCT CCCATCAAGA GCGTATCGCC 
TCTATTGCCC AGGCAGCCGA GCAGTTCGTC GCTGGAGGTC ACTTTGACGC CGACAACATC 
CGTCAGAAGC AGCAACAACT GCAGGGACGC TATTCTGCTT TGGAGCAGCC TATGCAGATG 
CGCAAGCAGC GCTTGCTGGA CTCGCTGCAG GTACAGCAGC TGTTCCGCGA TGTCGAAGAC 
GAGGAGGCCT GGATCCGCGA GAAGGAGCCT GTGGCAGCCA GCACCAACCG TGGTCGCGAT 
CTCATTGGTG TGCAGAACCT GCAAAAGAAG CACCAGGCCG TCCTTGCTGA AATCAACAAT 
CATGAGCCCC GAGTTGCAGC CGTCTGCGCA GCTGGCTCAC GCATGTTGAG CGATGGTCAT 
TTCGCAGCTG AAGAGATCAG CCAGAGACTG GCTAGTCTCG ATGAACATTG GGGACAGTTG 
AAGGAGAAGG CTCGCCAGCG TAAGAACGAC CTTGATGACT CGCTCCAGGC TCACCAATAC 
TTCGCCGATG CCAATGAAGC CGAGTCTTGG ATGAAGGAGA AGCGCCCCAT CGTCATGAAC 
GCCGATTACG GCAAAGACGA GGATAGTAGC GAGGCTCTGC TCAAGAAACA CGAGGCTCTC 
GTGAGCGATC TCGAAGCTTT TGCTAGCACC ATTGCAGCGC TCAAGGAACA GGCAGCCGCC 
TGCCGTCAGC AAGAGACACC TGCTGTCGAC GTAGCTGGAA AGGAATGCGT TGTCGCACTC 
TACGATTACA CCGAGAAGTC TCCTCGCGAG GTCTCTATGA AGAAAGGAGA CACCCTTACA 
CTCCTCAACA GCAGCAACAA GGACTGGTGG AAGGTGGAGG TAAACGATCG TCAGGGCTTC 
GTGCCAGCCG CCTATGTCAA GCGCGTAGAA CCGGACGCGG GTCTTAGTGC TTCTCAGCAG 
AATCTGGCTC GCGAGCAGGG CTCCATTGCC GCTCGCCAGG CCCAGATTGA AGCCCAATAC 
GAAGACTTGC TGCGATTGGC CAGAGAAAGA CAAAACAAGC TTAATGAAAC CGCCAAGGCG 
TACGTGTTGG TTCGCGAGGC CGCTGAACTG GCCACCTGGA TCAAGGGCAA GGAGAACCAT 
GCTCAGGTAC AGGATGTAGG CGAGGATTTG GAACAAGTCG AGGTTATGCA GAAGAAGTTC 
GACGATTTCC GAGCTGATCT GAAGGCAAAC GAGGTGCGTC TCGCAGAGAT GAACGAGATC 
GCCGTGCAGC TGATGAGCGT CGGCCAGACC GAGGCAGCTC TTAAGATCCA GACGCAGATC 
CAAGAGCTTA ACGAAAAATG GACGAGTCTG CAGACTCTAA CAGCCGAGCG CGCCAGTCAG 
CTGGACTCTG CTCACGAAGT ACAGCGCTTC CATCGCGATG TCGACGACAC CAAAGACTGG 
ATTCGCGAGA AGGATGCTGC CCTGAACAAC GATGACCTGG GCAAGGACTT GCGCAGCGTG 
CAAACTCTGC TCCGCAAGCA CGAAGGTCTG GAGCGCGACC TCGCTGCCCT TGGGGACAAG 
ATTAAGCAGT TGGACGAAAC AGCAAACCGC TTGATGCAGT CGCATCCTGA TACGGCCGAG 
CAAACCTACA CCAAGCAGAA GGAGATCAAC GAGGAATGGA CTCAGCTTAC TGCCAAAGCT 
GATTCTAGAA AGAAGAAGCT GCTCGATTCT TATGACCTGC AACGCTATCT CAGCGACTAT 
CGGGATCTTA TGGCCTGGAT TAATTCGATG ATGGGTCTGG TAGCTTCCGA AGAATTGGCT 
AACGACGTCA CCGGTGCTGA GGCTCTGCTT GAACGTCACC AGAATCACAG AACAGAAATC 
GATGCACGAT ATGGCATTGT TCCACAGGAA CATCGCATGG AAATCGACGC CCGCGTTGGA 
ACTTTCCAAG CTTTCGAGCT CTTCGGCCAG CAACTCTTGC AATCCAACCA CTATGCTAGC 
GTTGAGATAC AAGAGAAACT CGAATCTATG AACGAGGCTC GTCAAGAACT TGAGAAGGCT 
TGGATACAGC GCCGTATGCA GTTGGACCAG AACCTCGAGC TGCAACTCTT CTGCCGCGAT 
TGCGAACAGG CCGAGAACTG GATGTCCGCT CGTGAAGCTT TCCTTAGTAG CAGTAGCGCT 
GATGCCCTCG ACAGCGGTGA CAATGTAGAG GCCCTCATCA AGAAGCACGA GGACTTCGAC 
AAGGCTATCA ACGGTCACGA AGAGAAAATA GCCGCTCTTC AAACTCTGGC TGATCAGCTT 
ATTGCTGCTG AGCACTACGC TGCTCGACCT ATCGACGAAC GTCGCTGCCA GGTACTCGAT 
CGCTGGCGTC ACTTGAAAGA TGCTCTCATT GAGAAACGAT CACGGCTTGG CGAGTCCCAA 
ACTCTGCAGC AATTCTCTCG CGATGCTGAT GAGATGGAGA ATTGGATCGC TGAGAAGCTG 
CAGCTCGCCA CTGAAGAGAA CTACAAAGAT CCGGCTAATA TACAGTCCAA ACATCAAAAA 
CACCAAGCCT TTGAAGCTGA GCTTGCTGCA AATGCCGAAC GTATCTCGTC GGTGCTGGCT 
ATGGGTGGTA ACCTGATCGA AAAGCATCAG TGCGCTGGCT CTGAAGATGC CGTGCAGAAG 
CGTCTTGCCT CTATTGCCGA TCAGTGGGAG TACCTCACTC ACAAAACAAC CGAAAAATCC 
ATGAAACTAA AGGAAGCCAA TAAGCAACGC ACCTACATCG CTGCAGTCAA GGATTTAGAC 
TTTTGGCTCG GTGAGGTCGA GAGTCTGCTG ACAAGCGAAG ATGCTGGCAA GGATCTCGCC 
TCAGTGCAGA ACCTCATGAA GAAGCATCAA CTGGTTGAGG CCGACATCAA CGCCCACGAG 
GAGCGTATTA AAGACATGAA TGCTCAGGCT GACTCCCTCA TCGAGAGCGG TCAATTCGAG 
GCAGCGGGTA TCCAAGAAAA GCGTCAAAGC ATCAACGAAC GCTATGAACG CGTACGCAAC 
CTCGCAGCTC ACAGGCAGGC TAGACTCAAT GAGGCTAACA CCTTGCATCA GTTCTTCCGC 
GACATCGCCG ACGAGGAGTC CTGGATCAAG GAGAAGAAGC TTCTCGTTGG TTCAGATGAC 
TACGGTCGTG ACCTTACAGG CGTTCAGAAT TTAAAGAAAA AGCACAAGCG TCTCGAGGCT 
GAGCTTGGCA GCCACGAGCC CGCTATCCAA GCCGTACAAG AAGCTGGTGA GAAACTCATG 
GACGTCTCCA ACCTAGGCGT ACCAGAGATC GAGCAACGAC TCAAGCTTCT CGAACAGGCG 
TGGTCTGAAT TGAAACAACT AGCTGCCACG CGTGGTCAGA AGCTTGACGA GTCGCTGGCT 
TACCAGCAAT TTTTGGCAAA GGTTGAAGAG GAAGAAGCCT GGATCGCCGA GAAACAGCAA 
TTGCTCTCAC TCGAGGATTA CGGCGATACA ATGGCTGCCG TGCAAGGTTT GCTCAAGAAG 
CACGACGCTT TCGAGACAGA CTTTGCTGCC CACGGTGAAC GTTGCCGTGA AATCCGCGAA 
GCCGGTGAGG GACTCATAGC TGCAGGTAAC CATCGTGCTG AGGCAATCGA ACAGCGCTGC 
CAGCAGCTAG GAGCCAAGCT TGAACAGCTT GGAGCTTTGG CTGCTAGACG CAAGACTCGC 
CTCAACGATA ACTCAGCTTA CCTGCAGTTC ATGTGGAAGG CTGACGTAGT CGAGTCCTGG 
ATCGCTGACA AGGAAACACA CGTACGATCC GAAGAGTTTG GCCGTGATTT GTCCAGTGTG 
CAGACTTTGC TCACCAAACA GGAGACCTTC GACGCTGGTC TGCACGCTTT CGAACACGAG 
GGTATTCAGA ACATAACTTC TCTCAAGGAG ATGCTAATCG AGGCCGGTCA TGAACAGAGC 
CCCGCGATTC ACAAACGTCA CGCTGACGTT ATCGCTCGCT GGCAGAAGCT GCTTGCCGAT 
TCGCAAGCCC GTAAGCAGCG TCTCTTGCAG ATGCAGGAGC AATTCCGTCA GATAGAAGAG 
CTTTACCTGA CTTTCGCCAA GAAGGCATCG GCCTTCAACT CGTGGTTCGA AAACGCTGAG 
GAGGATTTGA CCGACCCTGT CCGCTGCAAC AGCATCGAAG AGATCCGTGC TTTGCGCGAA 
GCTCATGCGC AGTTCCAAGC TAGTCTGTCA TCGGCTGAGG CCGACTTCCA GGCTCTTGGA 
GCTCTTGACC GCCAAATCAA GAACTTCAAT GTTGGCCCCA ACCCTTACAC CTGGTTCACC 
ATGGAAGCGC TCGAGGACAC CTGGCGCAAC TTGCAGAAGA TCATCAAGGA GCGCGACGTC 
GAATTAGCTA AGGAAGCTCA GCGTCAAGAG GAAAACGACA AGTTGCGAAA GGAGTTCGCC 
AAGCACGCCA ATGCCTTCCA TCAGTGGCTC GCGGACACCC GTACTTCTAT GATGGAAGGA 
TCGGGCTCAC TTGAACAACA GCTCGAAGCA ACAAAGAGAA AGACACAAGA AGTCCGAGCG 
CGGCGTCAAG ATCTCAAAAA GATCGAGGAT TTGGGCGCAA TACTGGAAGA ACATTTGATT 
CTCGACAACA GGTACACAGA ACACAGTACT GTAGGACTGG CCCAACAATG GGACCAGCTC 
GACCAGTTGG GTATGCGTAT GCAGCATAAC CTGGAACAAC AGATTCAAGC TCGTAACCAA 
TCTGGAGTGT CGGAAGGAGC CCTCAAGGAG TTCTCGATGA TGTTCAAGCA TTACGACAAG 
GACAAGAGCG GACGACTAAA TCACCAAGAG TTCAAGTCGT GCCTCAGAGC TTTGGGTTAC 
GATCTACCTA TGGTGGAAGA AGGACAACCA GATCCAGAAT TCGAAAATAT TCTAGACATT 
GTCGACCCGA ACAGAGATGG CTTTGTTTCC TTACAAGATC ACATGGCATT CATGATCAGC 
AAGGAAACTG AGAATGTCCA AAGTTCACAA GAAATCGAAA ACGCCTTCCG AGCTATTACA 
GCTGCCGATC GTCCTTACGT CACGAAAGAG GAACTCTACG CTAACCTCAC GAAAGAAATG 
GCGGACTATT GTGTGGCGAG GATGAAACCA TACGTCGACC CGAAGACGGA ACGACCGATC 
ACCGGCGCAC TAGACTATAT AGAATTTACA CACACGTTAT TCCAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100116996 ( XM_016981977.2 ) cDNA ORF clone, Nasonia vitripennis(jewel wasp) -> XP_016837466.1 Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X1, mRNA.

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CloneID	ONd22430
Clone ID Related Accession (Same CDS sequence)	XM_016981977.1 , XM_016981977.2
Accession Version	XM_016981977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577030400000
Organism	Nasonia vitripennis(jewel wasp)
Product	spectrin alpha chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022279606.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 24, 2019 this sequence version replaced XM_016981977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nasonia vitripennis Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGC AGATCACCCC CAAGGAGGTG AAAATCCTCG AGAACCCAGA GGACATCCAG 
GAGAGGAGAG AGCAGGTTCT GGGACGTTAT GCCAATTTTA AGTCGGAGGC GCGCAGCAAG 
CGCGACAAAC TCGAGGACTC TCGTCGTTTC CAGTACTTTA AACGAGATGC AGATGAACTC 
GAGGGTTGGA TTTATGAGAA GTTGCAAGCT GCTTCCGATG AAAGCTACAA AGATCCGACC 
AACCTACAGG CAAAGATTCA AAAGCACCAG GCTTTTGAAG CCGAAGTCGC TGCTCATTCG 
AATGCGATTG TTCTATTAGA CAATACTGGA TCGGAAATGA TTGCTCAGCA CCACTTTGCT 
AGTGACATCA TTCGTAAAAG GCTTGAGGAA TTGCATCGCT TATGGGAATT GCTTCTTAAT 
CGACTGGCTG ACAAGGGATT GAAGCTGCAG CAGGCTTTGG TTCTTGTACA ATTCATTCGC 
CATTGCGATG AAGTCATGTT TTGGATACAT GACAAGGAAG TCTTCGTCAC CACTGATGAG 
TTTGGACAAG ATTTGGAGCA CGTAGAAGTA CTCCAAAGGA AATTTGACGA ATTCCAGAAA 
GACATGACCA GCCAAGAATA TCGTGTAACT GAAGTTAACG AATTAGCCGA TAAATTACTT 
TTGGACGCTC ATCCTGAGCG CGATACTATT TTAAGACGCA AAGAAGAATT GAATGAATCA 
TGGCAGCGAT TGAAACAACT CGCTATTCTG CGTCAAGAAA AATTGTTCGG TGCTCACGAA 
ATTCAGAGAT TCAATCGTGA TGCCGACGAG ACAATGGCTT GGATTGGAGA GAAAGACGCA 
GTACTGTCTT CCGATGATTT TGGTCGCGAT TTGGCTAGTG TACAGCTGCT CCAGCGTACT 
CACGAGAACA TTGAACGCGA TTTGGCTGCG CTCGAAGACA AGGTGGCTAC GCTTAGCACT 
GAAGCTGACA GACTAGCCGG CATCCATCAA GCCGAGCACT CTCAGCAAAT TCAGTCCAAG 
AGAGCAGAGA TTCTACAGTC CTGGGAATCG CTTACTGCTA AGGCAAAAGA ACGCCGTTTC 
AAGCTCGACG AGTCTTACTA CTTACATCGC TTCCTTGCTG ACTACCGCGA CCTTGTTTCA 
TGGATGAACA ACATGCGAGC AATCATCTCA GCTGATGAGC TTGCAAAGGA CGTGGCTGGA 
GCTGAGGCGC TTCTTGAACG TCACCAAGAG CACAAGGGCG AGATCGATGC TCGTGCCGAC 
AGCTTTGATT CAACCGGCCT CGCCGGAAGT AAGCTTCTCG AACGTCAACA CTACGCTGCG 
TCGGAGGTAG CCATCAAGCT CGAATCTCTC GCTGCTGACA AGGCTTCGTT GCTTGAACTT 
TGGGAAAAGC GACGCATTCT TTACGAGCAG TGCATGGATC TGCAGCTCTT TTACCGTGAC 
ACTGAACAAG CTGATGCCTG GATGGCCAAG CAGGAAGCTT TCCTCGCCAA CGAGGATCTT 
GGAGATTCCC TTGACAGCGT CGAGGCTCTC ATCAAGAAGC ACGAGGACTT TGACAAGTCC 
CTAGCTGCCC AGGAGGAAAA GATCAAGGCG CTTGACGAGT TTGCCAGCAA GCTCATCGAA 
GGCGAACATT ATGCGGCCGA TGAAGTAGCC CAGCGCCGGC AGGCTCTTTT GCAACGTCGC 
TCAGCCCTCC TTGAAAAGTC GGCTTTGCGC CGTCGTGCCC TTGAGGACGC CTACAAGCTG 
CAGCAGTTCG AGCGTGACTG CGATGAGACC AAAGGCTGGG TCAACGAGAA GCTTAAGTTC 
GCCACCGATG ACAGCTACCT CGACCCTACC AATCTCAATG GTAAGCTGCA GAAACACCAG 
AATTTCGAGC AGGAGCTTCA AGCAAACAAA ACGCGCATGC AAGAAATGGT CGCTCAGGGC 
CAGGAACTCA TCGAAGCCGA GCATTACGCT CGCGAGCGCA TCCAGTCGCG CATCAAGGAG 
ATCGTCGAGC TCTGGGAAAG TCTGACCGAA GCCACTGAGA AGAAGGGCAA CAAGCTTCAG 
GAAGCTGCGC AACAACAGCA ATTCAACCGC ACTGTCGAGG ACGTCGAACT CTGGCTCTCA 
GAGGTCGAAG GTCAGCTCTT GTCCGAAGAC TATGGCAAGG ACCTTACCAG CGTGCAGAAC 
CTTCAGAAAA AGCACGCCCT GCTCGAGGCC GACGTCGCCT CCCATCAAGA GCGTATCGCC 
TCTATTGCCC AGGCAGCCGA GCAGTTCGTC GCTGGAGGTC ACTTTGACGC CGACAACATC 
CGTCAGAAGC AGCAACAACT GCAGGGACGC TATTCTGCTT TGGAGCAGCC TATGCAGATG 
CGCAAGCAGC GCTTGCTGGA CTCGCTGCAG GTACAGCAGC TGTTCCGCGA TGTCGAAGAC 
GAGGAGGCCT GGATCCGCGA GAAGGAGCCT GTGGCAGCCA GCACCAACCG TGGTCGCGAT 
CTCATTGGTG TGCAGAACCT GCAAAAGAAG CACCAGGCCG TCCTTGCTGA AATCAACAAT 
CATGAGCCCC GAGTTGCAGC CGTCTGCGCA GCTGGCTCAC GCATGTTGAG CGATGGTCAT 
TTCGCAGCTG AAGAGATCAG CCAGAGACTG GCTAGTCTCG ATGAACATTG GGGACAGTTG 
AAGGAGAAGG CTCGCCAGCG TAAGAACGAC CTTGATGACT CGCTCCAGGC TCACCAATAC 
TTCGCCGATG CCAATGAAGC CGAGTCTTGG ATGAAGGAGA AGCGCCCCAT CGTCATGAAC 
GCCGATTACG GCAAAGACGA GGATAGTAGC GAGGCTCTGC TCAAGAAACA CGAGGCTCTC 
GTGAGCGATC TCGAAGCTTT TGCTAGCACC ATTGCAGCGC TCAAGGAACA GGCAGCCGCC 
TGCCGTCAGC AAGAGACACC TGCTGTCGAC GTAGCTGGAA AGGAATGCGT TGTCGCACTC 
TACGATTACA CCGAGAAGTC TCCTCGCGAG GTCTCTATGA AGAAAGGAGA CACCCTTACA 
CTCCTCAACA GCAGCAACAA GGACTGGTGG AAGGTGGAGG TAAACGATCG TCAGGGCTTC 
GTGCCAGCCG CCTATGTCAA GCGCGTAGAA CCGGACGCGG GTCTTAGTGC TTCTCAGCAG 
AATCTGGCTC GCGAGCAGGG CTCCATTGCC GCTCGCCAGG CCCAGATTGA AGCCCAATAC 
GAAGACTTGC TGCGATTGGC CAGAGAAAGA CAAAACAAGC TTAATGAAAC CGCCAAGGCG 
TACGTGTTGG TTCGCGAGGC CGCTGAACTG GCCACCTGGA TCAAGGGCAA GGAGAACCAT 
GCTCAGGTAC AGGATGTAGG CGAGGATTTG GAACAAGTCG AGGTTATGCA GAAGAAGTTC 
GACGATTTCC GAGCTGATCT GAAGGCAAAC GAGGTGCGTC TCGCAGAGAT GAACGAGATC 
GCCGTGCAGC TGATGAGCGT CGGCCAGACC GAGGCAGCTC TTAAGATCCA GACGCAGATC 
CAAGAGCTTA ACGAAAAATG GACGAGTCTG CAGACTCTAA CAGCCGAGCG CGCCAGTCAG 
CTGGACTCTG CTCACGAAGT ACAGCGCTTC CATCGCGATG TCGACGACAC CAAAGACTGG 
ATTCGCGAGA AGGATGCTGC CCTGAACAAC GATGACCTGG GCAAGGACTT GCGCAGCGTG 
CAAACTCTGC TCCGCAAGCA CGAAGGTCTG GAGCGCGACC TCGCTGCCCT TGGGGACAAG 
ATTAAGCAGT TGGACGAAAC AGCAAACCGC TTGATGCAGT CGCATCCTGA TACGGCCGAG 
CAAACCTACA CCAAGCAGAA GGAGATCAAC GAGGAATGGA CTCAGCTTAC TGCCAAAGCT 
GATTCTAGAA AGAAGAAGCT GCTCGATTCT TATGACCTGC AACGCTATCT CAGCGACTAT 
CGGGATCTTA TGGCCTGGAT TAATTCGATG ATGGGTCTGG TAGCTTCCGA AGAATTGGCT 
AACGACGTCA CCGGTGCTGA GGCTCTGCTT GAACGTCACC AGAATCACAG AACAGAAATC 
GATGCACGAT ATGGCATTGT TCCACAGGAA CATCGCATGG AAATCGACGC CCGCGTTGGA 
ACTTTCCAAG CTTTCGAGCT CTTCGGCCAG CAACTCTTGC AATCCAACCA CTATGCTAGC 
GTTGAGATAC AAGAGAAACT CGAATCTATG AACGAGGCTC GTCAAGAACT TGAGAAGGCT 
TGGATACAGC GCCGTATGCA GTTGGACCAG AACCTCGAGC TGCAACTCTT CTGCCGCGAT 
TGCGAACAGG CCGAGAACTG GATGTCCGCT CGTGAAGCTT TCCTTAGTAG CAGTAGCGCT 
GATGCCCTCG ACAGCGGTGA CAATGTAGAG GCCCTCATCA AGAAGCACGA GGACTTCGAC 
AAGGCTATCA ACGGTCACGA AGAGAAAATA GCCGCTCTTC AAACTCTGGC TGATCAGCTT 
ATTGCTGCTG AGCACTACGC TGCTCGACCT ATCGACGAAC GTCGCTGCCA GGTACTCGAT 
CGCTGGCGTC ACTTGAAAGA TGCTCTCATT GAGAAACGAT CACGGCTTGG CGAGTCCCAA 
ACTCTGCAGC AATTCTCTCG CGATGCTGAT GAGATGGAGA ATTGGATCGC TGAGAAGCTG 
CAGCTCGCCA CTGAAGAGAA CTACAAAGAT CCGGCTAATA TACAGTCCAA ACATCAAAAA 
CACCAAGCCT TTGAAGCTGA GCTTGCTGCA AATGCCGAAC GTATCTCGTC GGTGCTGGCT 
ATGGGTGGTA ACCTGATCGA AAAGCATCAG TGCGCTGGCT CTGAAGATGC CGTGCAGAAG 
CGTCTTGCCT CTATTGCCGA TCAGTGGGAG TACCTCACTC ACAAAACAAC CGAAAAATCC 
ATGAAACTAA AGGAAGCCAA TAAGCAACGC ACCTACATCG CTGCAGTCAA GGATTTAGAC 
TTTTGGCTCG GTGAGGTCGA GAGTCTGCTG ACAAGCGAAG ATGCTGGCAA GGATCTCGCC 
TCAGTGCAGA ACCTCATGAA GAAGCATCAA CTGGTTGAGG CCGACATCAA CGCCCACGAG 
GAGCGTATTA AAGACATGAA TGCTCAGGCT GACTCCCTCA TCGAGAGCGG TCAATTCGAG 
GCAGCGGGTA TCCAAGAAAA GCGTCAAAGC ATCAACGAAC GCTATGAACG CGTACGCAAC 
CTCGCAGCTC ACAGGCAGGC TAGACTCAAT GAGGCTAACA CCTTGCATCA GTTCTTCCGC 
GACATCGCCG ACGAGGAGTC CTGGATCAAG GAGAAGAAGC TTCTCGTTGG TTCAGATGAC 
TACGGTCGTG ACCTTACAGG CGTTCAGAAT TTAAAGAAAA AGCACAAGCG TCTCGAGGCT 
GAGCTTGGCA GCCACGAGCC CGCTATCCAA GCCGTACAAG AAGCTGGTGA GAAACTCATG 
GACGTCTCCA ACCTAGGCGT ACCAGAGATC GAGCAACGAC TCAAGCTTCT CGAACAGGCG 
TGGTCTGAAT TGAAACAACT AGCTGCCACG CGTGGTCAGA AGCTTGACGA GTCGCTGGCT 
TACCAGCAAT TTTTGGCAAA GGTTGAAGAG GAAGAAGCCT GGATCGCCGA GAAACAGCAA 
TTGCTCTCAC TCGAGGATTA CGGCGATACA ATGGCTGCCG TGCAAGGTTT GCTCAAGAAG 
CACGACGCTT TCGAGACAGA CTTTGCTGCC CACGGTGAAC GTTGCCGTGA AATCCGCGAA 
GCCGGTGAGG GACTCATAGC TGCAGGTAAC CATCGTGCTG AGGCAATCGA ACAGCGCTGC 
CAGCAGCTAG GAGCCAAGCT TGAACAGCTT GGAGCTTTGG CTGCTAGACG CAAGACTCGC 
CTCAACGATA ACTCAGCTTA CCTGCAGTTC ATGTGGAAGG CTGACGTAGT CGAGTCCTGG 
ATCGCTGACA AGGAAACACA CGTACGATCC GAAGAGTTTG GCCGTGATTT GTCCAGTGTG 
CAGACTTTGC TCACCAAACA GGAGACCTTC GACGCTGGTC TGCACGCTTT CGAACACGAG 
GGTATTCAGA ACATAACTTC TCTCAAGGAG ATGCTAATCG AGGCCGGTCA TGAACAGAGC 
CCCGCGATTC ACAAACGTCA CGCTGACGTT ATCGCTCGCT GGCAGAAGCT GCTTGCCGAT 
TCGCAAGCCC GTAAGCAGCG TCTCTTGCAG ATGCAGGAGC AATTCCGTCA GATAGAAGAG 
CTTTACCTGA CTTTCGCCAA GAAGGCATCG GCCTTCAACT CGTGGTTCGA AAACGCTGAG 
GAGGATTTGA CCGACCCTGT CCGCTGCAAC AGCATCGAAG AGATCCGTGC TTTGCGCGAA 
GCTCATGCGC AGTTCCAAGC TAGTCTGTCA TCGGCTGAGG CCGACTTCCA GGCTCTTGGA 
GCTCTTGACC GCCAAATCAA GAACTTCAAT GTTGGCCCCA ACCCTTACAC CTGGTTCACC 
ATGGAAGCGC TCGAGGACAC CTGGCGCAAC TTGCAGAAGA TCATCAAGGA GCGCGACGTC 
GAATTAGCTA AGGAAGCTCA GCGTCAAGAG GAAAACGACA AGTTGCGAAA GGAGTTCGCC 
AAGCACGCCA ATGCCTTCCA TCAGTGGCTC GCGGACACCC GTACTTCTAT GATGGAAGGA 
TCGGGCTCAC TTGAACAACA GCTCGAAGCA ACAAAGAGAA AGACACAAGA AGTCCGAGCG 
CGGCGTCAAG ATCTCAAAAA GATCGAGGAT TTGGGCGCAA TACTGGAAGA ACATTTGATT 
CTCGACAACA GGTACACAGA ACACAGTACT GTAGGACTGG CCCAACAATG GGACCAGCTC 
GACCAGTTGG GTATGCGTAT GCAGCATAAC CTGGAACAAC AGATTCAAGC TCGTAACCAA 
TCTGGAGTGT CGGAAGGAGC CCTCAAGGAG TTCTCGATGA TGTTCAAGCA TTACGACAAG 
GACAAGAGCG GACGACTAAA TCACCAAGAG TTCAAGTCGT GCCTCAGAGC TTTGGGTTAC 
GATCTACCTA TGGTGGAAGA AGGACAACCA GATCCAGAAT TCGAAAATAT TCTAGACATT 
GTCGACCCGA ACAGAGATGG CTTTGTTTCC TTACAAGATC ACATGGCATT CATGATCAGC 
AAGGAAACTG AGAATGTCCA AAGTTCACAA GAAATCGAAA ACGCCTTCCG AGCTATTACA 
GCTGCCGATC GTCCTTACGT CACGAAAGAG GAACTCTACG CTAACCTCAC GAAAGAAATG 
GCGGACTATT GTGTGGCGAG GATGAAACCA TACGTCGACC CGAAGACGGA ACGACCGATC 
ACCGGCGCAC TAGACTATAT AGAATTTACA CACACGTTAT TCCAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016837466.1
CDS	401..7711
Translation	MDQQITPKEVKILENPEDIQERREQVLGRYANFKSEARSKRDKLEDSRRFQYFKRDADELEGWIYEKLQAASDESYKDPTNLQAKIQKHQAFEAEVAAHSNAIVLLDNTGSEMIAQHHFASDIIRKRLEELHRLWELLLNRLADKGLKLQQALVLVQFIRHCDEVMFWIHDKEVFVTTDEFGQDLEHVEVLQRKFDEFQKDMTSQEYRVTEVNELADKLLLDAHPERDTILRRKEELNESWQRLKQLAILRQEKLFGAHEIQRFNRDADETMAWIGEKDAVLSSDDFGRDLASVQLLQRTHENIERDLAALEDKVATLSTEADRLAGIHQAEHSQQIQSKRAEILQSWESLTAKAKERRFKLDESYYLHRFLADYRDLVSWMNNMRAIISADELAKDVAGAEALLERHQEHKGEIDARADSFDSTGLAGSKLLERQHYAASEVAIKLESLAADKASLLELWEKRRILYEQCMDLQLFYRDTEQADAWMAKQEAFLANEDLGDSLDSVEALIKKHEDFDKSLAAQEEKIKALDEFASKLIEGEHYAADEVAQRRQALLQRRSALLEKSALRRRALEDAYKLQQFERDCDETKGWVNEKLKFATDDSYLDPTNLNGKLQKHQNFEQELQANKTRMQEMVAQGQELIEAEHYARERIQSRIKEIVELWESLTEATEKKGNKLQEAAQQQQFNRTVEDVELWLSEVEGQLLSEDYGKDLTSVQNLQKKHALLEADVASHQERIASIAQAAEQFVAGGHFDADNIRQKQQQLQGRYSALEQPMQMRKQRLLDSLQVQQLFRDVEDEEAWIREKEPVAASTNRGRDLIGVQNLQKKHQAVLAEINNHEPRVAAVCAAGSRMLSDGHFAAEEISQRLASLDEHWGQLKEKARQRKNDLDDSLQAHQYFADANEAESWMKEKRPIVMNADYGKDEDSSEALLKKHEALVSDLEAFASTIAALKEQAAACRQQETPAVDVAGKECVVALYDYTEKSPREVSMKKGDTLTLLNSSNKDWWKVEVNDRQGFVPAAYVKRVEPDAGLSASQQNLAREQGSIAARQAQIEAQYEDLLRLARERQNKLNETAKAYVLVREAAELATWIKGKENHAQVQDVGEDLEQVEVMQKKFDDFRADLKANEVRLAEMNEIAVQLMSVGQTEAALKIQTQIQELNEKWTSLQTLTAERASQLDSAHEVQRFHRDVDDTKDWIREKDAALNNDDLGKDLRSVQTLLRKHEGLERDLAALGDKIKQLDETANRLMQSHPDTAEQTYTKQKEINEEWTQLTAKADSRKKKLLDSYDLQRYLSDYRDLMAWINSMMGLVASEELANDVTGAEALLERHQNHRTEIDARYGIVPQEHRMEIDARVGTFQAFELFGQQLLQSNHYASVEIQEKLESMNEARQELEKAWIQRRMQLDQNLELQLFCRDCEQAENWMSAREAFLSSSSADALDSGDNVEALIKKHEDFDKAINGHEEKIAALQTLADQLIAAEHYAARPIDERRCQVLDRWRHLKDALIEKRSRLGESQTLQQFSRDADEMENWIAEKLQLATEENYKDPANIQSKHQKHQAFEAELAANAERISSVLAMGGNLIEKHQCAGSEDAVQKRLASIADQWEYLTHKTTEKSMKLKEANKQRTYIAAVKDLDFWLGEVESLLTSEDAGKDLASVQNLMKKHQLVEADINAHEERIKDMNAQADSLIESGQFEAAGIQEKRQSINERYERVRNLAAHRQARLNEANTLHQFFRDIADEESWIKEKKLLVGSDDYGRDLTGVQNLKKKHKRLEAELGSHEPAIQAVQEAGEKLMDVSNLGVPEIEQRLKLLEQAWSELKQLAATRGQKLDESLAYQQFLAKVEEEEAWIAEKQQLLSLEDYGDTMAAVQGLLKKHDAFETDFAAHGERCREIREAGEGLIAAGNHRAEAIEQRCQQLGAKLEQLGALAARRKTRLNDNSAYLQFMWKADVVESWIADKETHVRSEEFGRDLSSVQTLLTKQETFDAGLHAFEHEGIQNITSLKEMLIEAGHEQSPAIHKRHADVIARWQKLLADSQARKQRLLQMQEQFRQIEELYLTFAKKASAFNSWFENAEEDLTDPVRCNSIEEIRALREAHAQFQASLSSAEADFQALGALDRQIKNFNVGPNPYTWFTMEALEDTWRNLQKIIKERDVELAKEAQRQEENDKLRKEFAKHANAFHQWLADTRTSMMEGSGSLEQQLEATKRKTQEVRARRQDLKKIEDLGAILEEHLILDNRYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNQSGVSEGALKEFSMMFKHYDKDKSGRLNHQEFKSCLRALGYDLPMVEEGQPDPEFENILDIVDPNRDGFVSLQDHMAFMISKETENVQSSQEIENAFRAITAADRPYVTKEELYANLTKEMADYCVARMKPYVDPKTERPITGALDYIEFTHTLFQN

Target ORF information:

RefSeq Version	XM_016981977.2
Organism	Nasonia vitripennis(jewel wasp)
Definition	Nasonia vitripennis spectrin alpha chain (LOC100116996), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016981977.2

ORF Insert Sequence:

ATGGATCAGC AGATCACCCC CAAGGAGGTG AAAATCCTCG AGAACCCAGA GGACATCCAG 
GAGAGGAGAG AGCAGGTTCT GGGACGTTAT GCCAATTTTA AGTCGGAGGC GCGCAGCAAG 
CGCGACAAAC TCGAGGACTC TCGTCGTTTC CAGTACTTTA AACGAGATGC AGATGAACTC 
GAGGGTTGGA TTTATGAGAA GTTGCAAGCT GCTTCCGATG AAAGCTACAA AGATCCGACC 
AACCTACAGG CAAAGATTCA AAAGCACCAG GCTTTTGAAG CCGAAGTCGC TGCTCATTCG 
AATGCGATTG TTCTATTAGA CAATACTGGA TCGGAAATGA TTGCTCAGCA CCACTTTGCT 
AGTGACATCA TTCGTAAAAG GCTTGAGGAA TTGCATCGCT TATGGGAATT GCTTCTTAAT 
CGACTGGCTG ACAAGGGATT GAAGCTGCAG CAGGCTTTGG TTCTTGTACA ATTCATTCGC 
CATTGCGATG AAGTCATGTT TTGGATACAT GACAAGGAAG TCTTCGTCAC CACTGATGAG 
TTTGGACAAG ATTTGGAGCA CGTAGAAGTA CTCCAAAGGA AATTTGACGA ATTCCAGAAA 
GACATGACCA GCCAAGAATA TCGTGTAACT GAAGTTAACG AATTAGCCGA TAAATTACTT 
TTGGACGCTC ATCCTGAGCG CGATACTATT TTAAGACGCA AAGAAGAATT GAATGAATCA 
TGGCAGCGAT TGAAACAACT CGCTATTCTG CGTCAAGAAA AATTGTTCGG TGCTCACGAA 
ATTCAGAGAT TCAATCGTGA TGCCGACGAG ACAATGGCTT GGATTGGAGA GAAAGACGCA 
GTACTGTCTT CCGATGATTT TGGTCGCGAT TTGGCTAGTG TACAGCTGCT CCAGCGTACT 
CACGAGAACA TTGAACGCGA TTTGGCTGCG CTCGAAGACA AGGTGGCTAC GCTTAGCACT 
GAAGCTGACA GACTAGCCGG CATCCATCAA GCCGAGCACT CTCAGCAAAT TCAGTCCAAG 
AGAGCAGAGA TTCTACAGTC CTGGGAATCG CTTACTGCTA AGGCAAAAGA ACGCCGTTTC 
AAGCTCGACG AGTCTTACTA CTTACATCGC TTCCTTGCTG ACTACCGCGA CCTTGTTTCA 
TGGATGAACA ACATGCGAGC AATCATCTCA GCTGATGAGC TTGCAAAGGA CGTGGCTGGA 
GCTGAGGCGC TTCTTGAACG TCACCAAGAG CACAAGGGCG AGATCGATGC TCGTGCCGAC 
AGCTTTGATT CAACCGGCCT CGCCGGAAGT AAGCTTCTCG AACGTCAACA CTACGCTGCG 
TCGGAGGTAG CCATCAAGCT CGAATCTCTC GCTGCTGACA AGGCTTCGTT GCTTGAACTT 
TGGGAAAAGC GACGCATTCT TTACGAGCAG TGCATGGATC TGCAGCTCTT TTACCGTGAC 
ACTGAACAAG CTGATGCCTG GATGGCCAAG CAGGAAGCTT TCCTCGCCAA CGAGGATCTT 
GGAGATTCCC TTGACAGCGT CGAGGCTCTC ATCAAGAAGC ACGAGGACTT TGACAAGTCC 
CTAGCTGCCC AGGAGGAAAA GATCAAGGCG CTTGACGAGT TTGCCAGCAA GCTCATCGAA 
GGCGAACATT ATGCGGCCGA TGAAGTAGCC CAGCGCCGGC AGGCTCTTTT GCAACGTCGC 
TCAGCCCTCC TTGAAAAGTC GGCTTTGCGC CGTCGTGCCC TTGAGGACGC CTACAAGCTG 
CAGCAGTTCG AGCGTGACTG CGATGAGACC AAAGGCTGGG TCAACGAGAA GCTTAAGTTC 
GCCACCGATG ACAGCTACCT CGACCCTACC AATCTCAATG GTAAGCTGCA GAAACACCAG 
AATTTCGAGC AGGAGCTTCA AGCAAACAAA ACGCGCATGC AAGAAATGGT CGCTCAGGGC 
CAGGAACTCA TCGAAGCCGA GCATTACGCT CGCGAGCGCA TCCAGTCGCG CATCAAGGAG 
ATCGTCGAGC TCTGGGAAAG TCTGACCGAA GCCACTGAGA AGAAGGGCAA CAAGCTTCAG 
GAAGCTGCGC AACAACAGCA ATTCAACCGC ACTGTCGAGG ACGTCGAACT CTGGCTCTCA 
GAGGTCGAAG GTCAGCTCTT GTCCGAAGAC TATGGCAAGG ACCTTACCAG CGTGCAGAAC 
CTTCAGAAAA AGCACGCCCT GCTCGAGGCC GACGTCGCCT CCCATCAAGA GCGTATCGCC 
TCTATTGCCC AGGCAGCCGA GCAGTTCGTC GCTGGAGGTC ACTTTGACGC CGACAACATC 
CGTCAGAAGC AGCAACAACT GCAGGGACGC TATTCTGCTT TGGAGCAGCC TATGCAGATG 
CGCAAGCAGC GCTTGCTGGA CTCGCTGCAG GTACAGCAGC TGTTCCGCGA TGTCGAAGAC 
GAGGAGGCCT GGATCCGCGA GAAGGAGCCT GTGGCAGCCA GCACCAACCG TGGTCGCGAT 
CTCATTGGTG TGCAGAACCT GCAAAAGAAG CACCAGGCCG TCCTTGCTGA AATCAACAAT 
CATGAGCCCC GAGTTGCAGC CGTCTGCGCA GCTGGCTCAC GCATGTTGAG CGATGGTCAT 
TTCGCAGCTG AAGAGATCAG CCAGAGACTG GCTAGTCTCG ATGAACATTG GGGACAGTTG 
AAGGAGAAGG CTCGCCAGCG TAAGAACGAC CTTGATGACT CGCTCCAGGC TCACCAATAC 
TTCGCCGATG CCAATGAAGC CGAGTCTTGG ATGAAGGAGA AGCGCCCCAT CGTCATGAAC 
GCCGATTACG GCAAAGACGA GGATAGTAGC GAGGCTCTGC TCAAGAAACA CGAGGCTCTC 
GTGAGCGATC TCGAAGCTTT TGCTAGCACC ATTGCAGCGC TCAAGGAACA GGCAGCCGCC 
TGCCGTCAGC AAGAGACACC TGCTGTCGAC GTAGCTGGAA AGGAATGCGT TGTCGCACTC 
TACGATTACA CCGAGAAGTC TCCTCGCGAG GTCTCTATGA AGAAAGGAGA CACCCTTACA 
CTCCTCAACA GCAGCAACAA GGACTGGTGG AAGGTGGAGG TAAACGATCG TCAGGGCTTC 
GTGCCAGCCG CCTATGTCAA GCGCGTAGAA CCGGACGCGG GTCTTAGTGC TTCTCAGCAG 
AATCTGGCTC GCGAGCAGGG CTCCATTGCC GCTCGCCAGG CCCAGATTGA AGCCCAATAC 
GAAGACTTGC TGCGATTGGC CAGAGAAAGA CAAAACAAGC TTAATGAAAC CGCCAAGGCG 
TACGTGTTGG TTCGCGAGGC CGCTGAACTG GCCACCTGGA TCAAGGGCAA GGAGAACCAT 
GCTCAGGTAC AGGATGTAGG CGAGGATTTG GAACAAGTCG AGGTTATGCA GAAGAAGTTC 
GACGATTTCC GAGCTGATCT GAAGGCAAAC GAGGTGCGTC TCGCAGAGAT GAACGAGATC 
GCCGTGCAGC TGATGAGCGT CGGCCAGACC GAGGCAGCTC TTAAGATCCA GACGCAGATC 
CAAGAGCTTA ACGAAAAATG GACGAGTCTG CAGACTCTAA CAGCCGAGCG CGCCAGTCAG 
CTGGACTCTG CTCACGAAGT ACAGCGCTTC CATCGCGATG TCGACGACAC CAAAGACTGG 
ATTCGCGAGA AGGATGCTGC CCTGAACAAC GATGACCTGG GCAAGGACTT GCGCAGCGTG 
CAAACTCTGC TCCGCAAGCA CGAAGGTCTG GAGCGCGACC TCGCTGCCCT TGGGGACAAG 
ATTAAGCAGT TGGACGAAAC AGCAAACCGC TTGATGCAGT CGCATCCTGA TACGGCCGAG 
CAAACCTACA CCAAGCAGAA GGAGATCAAC GAGGAATGGA CTCAGCTTAC TGCCAAAGCT 
GATTCTAGAA AGAAGAAGCT GCTCGATTCT TATGACCTGC AACGCTATCT CAGCGACTAT 
CGGGATCTTA TGGCCTGGAT TAATTCGATG ATGGGTCTGG TAGCTTCCGA AGAATTGGCT 
AACGACGTCA CCGGTGCTGA GGCTCTGCTT GAACGTCACC AGAATCACAG AACAGAAATC 
GATGCACGAT ATGGCATTGT TCCACAGGAA CATCGCATGG AAATCGACGC CCGCGTTGGA 
ACTTTCCAAG CTTTCGAGCT CTTCGGCCAG CAACTCTTGC AATCCAACCA CTATGCTAGC 
GTTGAGATAC AAGAGAAACT CGAATCTATG AACGAGGCTC GTCAAGAACT TGAGAAGGCT 
TGGATACAGC GCCGTATGCA GTTGGACCAG AACCTCGAGC TGCAACTCTT CTGCCGCGAT 
TGCGAACAGG CCGAGAACTG GATGTCCGCT CGTGAAGCTT TCCTTAGTAG CAGTAGCGCT 
GATGCCCTCG ACAGCGGTGA CAATGTAGAG GCCCTCATCA AGAAGCACGA GGACTTCGAC 
AAGGCTATCA ACGGTCACGA AGAGAAAATA GCCGCTCTTC AAACTCTGGC TGATCAGCTT 
ATTGCTGCTG AGCACTACGC TGCTCGACCT ATCGACGAAC GTCGCTGCCA GGTACTCGAT 
CGCTGGCGTC ACTTGAAAGA TGCTCTCATT GAGAAACGAT CACGGCTTGG CGAGTCCCAA 
ACTCTGCAGC AATTCTCTCG CGATGCTGAT GAGATGGAGA ATTGGATCGC TGAGAAGCTG 
CAGCTCGCCA CTGAAGAGAA CTACAAAGAT CCGGCTAATA TACAGTCCAA ACATCAAAAA 
CACCAAGCCT TTGAAGCTGA GCTTGCTGCA AATGCCGAAC GTATCTCGTC GGTGCTGGCT 
ATGGGTGGTA ACCTGATCGA AAAGCATCAG TGCGCTGGCT CTGAAGATGC CGTGCAGAAG 
CGTCTTGCCT CTATTGCCGA TCAGTGGGAG TACCTCACTC ACAAAACAAC CGAAAAATCC 
ATGAAACTAA AGGAAGCCAA TAAGCAACGC ACCTACATCG CTGCAGTCAA GGATTTAGAC 
TTTTGGCTCG GTGAGGTCGA GAGTCTGCTG ACAAGCGAAG ATGCTGGCAA GGATCTCGCC 
TCAGTGCAGA ACCTCATGAA GAAGCATCAA CTGGTTGAGG CCGACATCAA CGCCCACGAG 
GAGCGTATTA AAGACATGAA TGCTCAGGCT GACTCCCTCA TCGAGAGCGG TCAATTCGAG 
GCAGCGGGTA TCCAAGAAAA GCGTCAAAGC ATCAACGAAC GCTATGAACG CGTACGCAAC 
CTCGCAGCTC ACAGGCAGGC TAGACTCAAT GAGGCTAACA CCTTGCATCA GTTCTTCCGC 
GACATCGCCG ACGAGGAGTC CTGGATCAAG GAGAAGAAGC TTCTCGTTGG TTCAGATGAC 
TACGGTCGTG ACCTTACAGG CGTTCAGAAT TTAAAGAAAA AGCACAAGCG TCTCGAGGCT 
GAGCTTGGCA GCCACGAGCC CGCTATCCAA GCCGTACAAG AAGCTGGTGA GAAACTCATG 
GACGTCTCCA ACCTAGGCGT ACCAGAGATC GAGCAACGAC TCAAGCTTCT CGAACAGGCG 
TGGTCTGAAT TGAAACAACT AGCTGCCACG CGTGGTCAGA AGCTTGACGA GTCGCTGGCT 
TACCAGCAAT TTTTGGCAAA GGTTGAAGAG GAAGAAGCCT GGATCGCCGA GAAACAGCAA 
TTGCTCTCAC TCGAGGATTA CGGCGATACA ATGGCTGCCG TGCAAGGTTT GCTCAAGAAG 
CACGACGCTT TCGAGACAGA CTTTGCTGCC CACGGTGAAC GTTGCCGTGA AATCCGCGAA 
GCCGGTGAGG GACTCATAGC TGCAGGTAAC CATCGTGCTG AGGCAATCGA ACAGCGCTGC 
CAGCAGCTAG GAGCCAAGCT TGAACAGCTT GGAGCTTTGG CTGCTAGACG CAAGACTCGC 
CTCAACGATA ACTCAGCTTA CCTGCAGTTC ATGTGGAAGG CTGACGTAGT CGAGTCCTGG 
ATCGCTGACA AGGAAACACA CGTACGATCC GAAGAGTTTG GCCGTGATTT GTCCAGTGTG 
CAGACTTTGC TCACCAAACA GGAGACCTTC GACGCTGGTC TGCACGCTTT CGAACACGAG 
GGTATTCAGA ACATAACTTC TCTCAAGGAG ATGCTAATCG AGGCCGGTCA TGAACAGAGC 
CCCGCGATTC ACAAACGTCA CGCTGACGTT ATCGCTCGCT GGCAGAAGCT GCTTGCCGAT 
TCGCAAGCCC GTAAGCAGCG TCTCTTGCAG ATGCAGGAGC AATTCCGTCA GATAGAAGAG 
CTTTACCTGA CTTTCGCCAA GAAGGCATCG GCCTTCAACT CGTGGTTCGA AAACGCTGAG 
GAGGATTTGA CCGACCCTGT CCGCTGCAAC AGCATCGAAG AGATCCGTGC TTTGCGCGAA 
GCTCATGCGC AGTTCCAAGC TAGTCTGTCA TCGGCTGAGG CCGACTTCCA GGCTCTTGGA 
GCTCTTGACC GCCAAATCAA GAACTTCAAT GTTGGCCCCA ACCCTTACAC CTGGTTCACC 
ATGGAAGCGC TCGAGGACAC CTGGCGCAAC TTGCAGAAGA TCATCAAGGA GCGCGACGTC 
GAATTAGCTA AGGAAGCTCA GCGTCAAGAG GAAAACGACA AGTTGCGAAA GGAGTTCGCC 
AAGCACGCCA ATGCCTTCCA TCAGTGGCTC GCGGACACCC GTACTTCTAT GATGGAAGGA 
TCGGGCTCAC TTGAACAACA GCTCGAAGCA ACAAAGAGAA AGACACAAGA AGTCCGAGCG 
CGGCGTCAAG ATCTCAAAAA GATCGAGGAT TTGGGCGCAA TACTGGAAGA ACATTTGATT 
CTCGACAACA GGTACACAGA ACACAGTACT GTAGGACTGG CCCAACAATG GGACCAGCTC 
GACCAGTTGG GTATGCGTAT GCAGCATAAC CTGGAACAAC AGATTCAAGC TCGTAACCAA 
TCTGGAGTGT CGGAAGGAGC CCTCAAGGAG TTCTCGATGA TGTTCAAGCA TTACGACAAG 
GACAAGAGCG GACGACTAAA TCACCAAGAG TTCAAGTCGT GCCTCAGAGC TTTGGGTTAC 
GATCTACCTA TGGTGGAAGA AGGACAACCA GATCCAGAAT TCGAAAATAT TCTAGACATT 
GTCGACCCGA ACAGAGATGG CTTTGTTTCC TTACAAGATC ACATGGCATT CATGATCAGC 
AAGGAAACTG AGAATGTCCA AAGTTCACAA GAAATCGAAA ACGCCTTCCG AGCTATTACA 
GCTGCCGATC GTCCTTACGT CACGAAAGAG GAACTCTACG CTAACCTCAC GAAAGAAATG 
GCGGACTATT GTGTGGCGAG GATGAAACCA TACGTCGACC CGAAGACGGA ACGACCGATC 
ACCGGCGCAC TAGACTATAT AGAATTTACA CACACGTTAT TCCAGAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gene@genscript.com

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1-877-436-7274

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LOC100116996 cDNA ORF clone, Nasonia vitripennis(jewel wasp)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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PHONE

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