Brsk2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brsk2
Entrez Gene ID	75770
Full Name	BR serine/threonine kinase 2
Synonyms	4833424K13Rik,SAD-A,SADA
General protein information	Preferred Names BR serine/threonine kinase 2 Names serine/threonine-protein kinase BRSK2 BR serine/threonine-protein kinase 2 brain-specific serine/threonine-protein kinase 2 serine/threonine-protein kinase SAD-A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009930.3	NP_001009930.1	serine/threonine-protein kinase BRSK2 isoform gamma
NM_001276763.1	NP_001263692.1	serine/threonine-protein kinase BRSK2 isoform delta
NM_029426.2	NP_083702.1	serine/threonine-protein kinase BRSK2 isoform alpha
NM_001009929.3	NP_001009929.1	serine/threonine-protein kinase BRSK2 isoform beta

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012244	XP_320298.4
Canis lupus familiaris (dog)	BRSK2	XP_005631383.1
Homo sapiens (human)	BRSK2	NP_001243556.1
Caenorhabditis elegans (roundworm)	sad-1	NP_001076761.1
Macaca mulatta (Rhesus monkey)	BRSK2	XP_002799482.1
Mus musculus (house mouse)	Brsk2	NP_001009930.1
Rattus norvegicus (Norway rat)	Brsk2	XP_006230671.1
Gallus gallus (chicken)	BRSK2	NP_001186525.1
Drosophila melanogaster (fruit fly)	sff	NP_001261901.1
Danio rerio (zebrafish)	LOC100334759	XP_002666904.2
Danio rerio (zebrafish)	LOC100149571	XP_005166543.1
Bos taurus (cattle)	BRSK2	XP_002699458.3
Xenopus tropicalis (tropical clawed frog)	brsk2	XP_004913517.1

Gene Ontology
Bibliography

Related articles in PubMed

SAD-A, a downstream mediator of GLP-1 signaling, promotes the phosphorylation of Bad S155 to regulate in vitro β-cell functions.
Wang K, Liu D, Zhang Y, Chang X, Xu R, Pang J, Li K, Sun P, Zhu Y, Han X
Biochemical and biophysical research communications509(1)76-81(2019 01)

SAD-A Promotes Glucose-Stimulated Insulin Secretion Through Phosphorylation and Inhibition of GDIα in Male Islet β Cells.
Nie J, Sun C, Chang Z, Musi N, Shi Y
Endocrinology159(8)3036-3047(2018 08)

The loss of the kinases SadA and SadB results in early neuronal apoptosis and a reduced number of progenitors.
Dhumale P, Menon S, Chiang J, Püschel AW
PloS one13(4)e0196698(2018)

Structural insight into the mechanism of synergistic autoinhibition of SAD kinases.
Wu JX, Cheng YS, Wang J, Chen L, Ding M, Wu JW
Nature communications68953(2015 Dec)

LKB1 and AMPK regulate synaptic remodeling in old age.
Samuel MA, Voinescu PE, Lilley BN, de Cabo R, Foretz M, Viollet B, Pawlyk B, Sandberg MA, Vavvas DG, Sanes JR
Nature neuroscience17(9)1190-7(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Brsk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brsk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15777	NM_029426.2 Latest version!	Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu15811	NM_001009930.3 Latest version!	Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu15973	NM_001009929.3 Latest version!	Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu16581	NM_001276763.1 Latest version!	Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brsk2 ( NM_029426.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083702.1 Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15777
Clone ID Related Accession (Same CDS sequence)	NM_029426.2
Accession Version	NM_029426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK2 isoform alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772165.15 and AL603836.13. On Nov 17, 2006 this sequence version replaced NM_029426.1. Transcript Variant: This variant (1) lacks an alternate in-frame 3' exon and uses an alternate splice junction at the 5' end of the last exon compared to variant 3. The resulting isoform (alpha) has a shorter and distinct C-terminus compared to isoform gamma. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY660739.1, AY533672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 
CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 
GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 
CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 
AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 
GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 
AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 
AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 
TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 
CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 
TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 
AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 
CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 
ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 
GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 
CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 
CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 
CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 
CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 
CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 
GCCATTGAGA TGGCCCAGCA TGGCCAGAGA TCTCGATCCA TCAGTGGTGC GTCCTCAGGC 
CTTTCTACAA GTCCACTCAG CAGTCCTCGG GTGACCCCTC ACCCCTCACC AAGGGGTAGT 
CCCCTTCCTA CCCCCAAAGG GACGCCTGTC CACACGCCAA AGGAGAGCCC AGCTGGCACA 
CCCAACCCCA CACCACCATC CAGCCCTAGT GTTGGAGGAG TGCCCTGGCG GACACGACTG 
AACTCCATCA AGAACAGCTT CCTGGGCTCA CCTCGATTCC ACCGCCGGAA ACTCCAAGTT 
CCCACGCCAG AGGAGATGTC CAACCTGACC CCAGAATCCT CTCCAGAGCT GGCCAAGAAA 
TCGTGGTTCG GGAACTTCAT CAACCTGGAG AAGGAGGAGC AGATCTTTGT GGTGATCAAG 
GACAAGCCCC TGAGCTCCAT CAAGGCTGAC ATCGTTCATG CCTTCCTGTC GATCCCCAGC 
CTCAGCCACA GCGTTATTTC CCAGACAAGC TTCAGGGCTG AATACAAGGC CACAGGGGGC 
CCAGCAGTGT TCCAGAAGCC GGTCAAGTTC CAGGTGGACA TCACCTACAC TGAGGGCGGA 
GAGGCCCAGA AGGAGAATGG CATCTACTCA GTCACATTCA CTTTACTCTC AGGCCCCAGT 
CGCCGCTTCA AGAGGGTGGT GGAGACCATC CAGGCCCAGC TGTTAAGCAC CCATGACCAG 
CCATCAGCCC AGCACCTGTC AGGAATTATC CCGAAAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083702.1
CDS	336..2297
Translation	MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSHEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDQPSAQHLSGIIPKS

Target ORF information:

RefSeq Version	NM_029426.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029426.2

ORF Insert Sequence:

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 
CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 
GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 
CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 
AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 
GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 
AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 
AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 
TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 
CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 
TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 
AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 
CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 
ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 
GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 
CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 
CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 
CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 
CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 
CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 
GCCATTGAGA TGGCCCAGCA TGGCCAGAGA TCTCGATCCA TCAGTGGTGC GTCCTCAGGC 
CTTTCTACAA GTCCACTCAG CAGTCCTCGG GTGACCCCTC ACCCCTCACC AAGGGGTAGT 
CCCCTTCCTA CCCCCAAAGG GACGCCTGTC CACACGCCAA AGGAGAGCCC AGCTGGCACA 
CCCAACCCCA CACCACCATC CAGCCCTAGT GTTGGAGGAG TGCCCTGGCG GACACGACTG 
AACTCCATCA AGAACAGCTT CCTGGGCTCA CCTCGATTCC ACCGCCGGAA ACTCCAAGTT 
CCCACGCCAG AGGAGATGTC CAACCTGACC CCAGAATCCT CTCCAGAGCT GGCCAAGAAA 
TCGTGGTTCG GGAACTTCAT CAACCTGGAG AAGGAGGAGC AGATCTTTGT GGTGATCAAG 
GACAAGCCCC TGAGCTCCAT CAAGGCTGAC ATCGTTCATG CCTTCCTGTC GATCCCCAGC 
CTCAGCCACA GCGTTATTTC CCAGACAAGC TTCAGGGCTG AATACAAGGC CACAGGGGGC 
CCAGCAGTGT TCCAGAAGCC GGTCAAGTTC CAGGTGGACA TCACCTACAC TGAGGGCGGA 
GAGGCCCAGA AGGAGAATGG CATCTACTCA GTCACATTCA CTTTACTCTC AGGCCCCAGT 
CGCCGCTTCA AGAGGGTGGT GGAGACCATC CAGGCCCAGC TGTTAAGCAC CCATGACCAG 
CCATCAGCCC AGCACCTGTC AGGAATTATC CCGAAAAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk2 ( NM_001009930.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001009930.1 Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15811
Clone ID Related Accession (Same CDS sequence)	NM_001009930.3
Accession Version	NM_001009930.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570896000000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK2 isoform gamma
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772165.15, AK158574.1, AL603836.13 and BE986320.1. On Oct 13, 2012 this sequence version replaced NM_001009930.2. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (gamma). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY533674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 
CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 
GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 
CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 
AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 
GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 
AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 
AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 
TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 
CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 
TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 
AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 
CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 
ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 
GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 
CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 
CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 
CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 
CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 
CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 
GCCATTGAGA TGGCCCAGCA TGGCCAGAGA TCTCGATCCA TCAGTGGTGC GTCCTCAGGC 
CTTTCTACAA GTCCACTCAG CAGTCCTCGG GTGACCCCTC ACCCCTCACC AAGGGGTAGT 
CCCCTTCCTA CCCCCAAAGG GACGCCTGTC CACACGCCAA AGGAGAGCCC AGCTGGCACA 
CCCAACCCCA CACCACCATC CAGCCCTAGT GTTGGAGGAG TGCCCTGGCG GACACGACTG 
AACTCCATCA AGAACAGCTT CCTGGGCTCA CCTCGATTCC ACCGCCGGAA ACTCCAAGTT 
CCCACGCCAG AGGAGATGTC CAACCTGACC CCAGAATCCT CTCCAGAGCT GGCCAAGAAA 
TCGTGGTTCG GGAACTTCAT CAACCTGGAG AAGGAGGAGC AGATCTTTGT GGTGATCAAG 
GACAAGCCCC TGAGCTCCAT CAAGGCTGAC ATCGTTCATG CCTTCCTGTC GATCCCCAGC 
CTCAGCCACA GCGTTATTTC CCAGACAAGC TTCAGGGCTG AATACAAGGC CACAGGGGGC 
CCAGCAGTGT TCCAGAAGCC GGTCAAGTTC CAGGTGGACA TCACCTACAC TGAGGGCGGA 
GAGGCCCAGA AGGAGAATGG CATCTACTCA GTCACATTCA CTTTACTCTC AGGCCCCAGT 
CGCCGCTTCA AGAGGGTGGT GGAGACCATC CAGGCCCAGC TGTTAAGCAC CCATGACCAG 
CCATCAGCCC AGCACCTGTC AGACACCACT AACTGTATGG AAGTGATGAC GGGGCGGCTT 
TCCAAATGTG ACGAGAAGAA CGGGCAGGCG GCCCAGGCCC CCAGCACACC CGCCAAGCGG 
AGTGCCCACG GCCCCCTGGG TGACTCCGCG GCCGCTGGCC CTGGAGGGGA CACCGAGTAC 
CCGATGGGCA AGGACATGGC CAAGATGGGG CCGCCCGCCG CCCGCCGTGA GCAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009930.1
CDS	336..2495
Translation	MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSHEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDQPSAQHLSDTTNCMEVMTGRLSKCDEKNGQAAQAPSTPAKRSAHGPLGDSAAAGPGGDTEYPMGKDMAKMGPPAARREQP

Target ORF information:

RefSeq Version	NM_001009930.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009930.3

ORF Insert Sequence:

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 
CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 
GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 
CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 
AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 
GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 
AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 
AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 
TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 
CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 
TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 
AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 
CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 
ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 
GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 
CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 
CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 
CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 
CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 
CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 
GCCATTGAGA TGGCCCAGCA TGGCCAGAGA TCTCGATCCA TCAGTGGTGC GTCCTCAGGC 
CTTTCTACAA GTCCACTCAG CAGTCCTCGG GTGACCCCTC ACCCCTCACC AAGGGGTAGT 
CCCCTTCCTA CCCCCAAAGG GACGCCTGTC CACACGCCAA AGGAGAGCCC AGCTGGCACA 
CCCAACCCCA CACCACCATC CAGCCCTAGT GTTGGAGGAG TGCCCTGGCG GACACGACTG 
AACTCCATCA AGAACAGCTT CCTGGGCTCA CCTCGATTCC ACCGCCGGAA ACTCCAAGTT 
CCCACGCCAG AGGAGATGTC CAACCTGACC CCAGAATCCT CTCCAGAGCT GGCCAAGAAA 
TCGTGGTTCG GGAACTTCAT CAACCTGGAG AAGGAGGAGC AGATCTTTGT GGTGATCAAG 
GACAAGCCCC TGAGCTCCAT CAAGGCTGAC ATCGTTCATG CCTTCCTGTC GATCCCCAGC 
CTCAGCCACA GCGTTATTTC CCAGACAAGC TTCAGGGCTG AATACAAGGC CACAGGGGGC 
CCAGCAGTGT TCCAGAAGCC GGTCAAGTTC CAGGTGGACA TCACCTACAC TGAGGGCGGA 
GAGGCCCAGA AGGAGAATGG CATCTACTCA GTCACATTCA CTTTACTCTC AGGCCCCAGT 
CGCCGCTTCA AGAGGGTGGT GGAGACCATC CAGGCCCAGC TGTTAAGCAC CCATGACCAG 
CCATCAGCCC AGCACCTGTC AGACACCACT AACTGTATGG AAGTGATGAC GGGGCGGCTT 
TCCAAATGTG ACGAGAAGAA CGGGCAGGCG GCCCAGGCCC CCAGCACACC CGCCAAGCGG 
AGTGCCCACG GCCCCCTGGG TGACTCCGCG GCCGCTGGCC CTGGAGGGGA CACCGAGTAC 
CCGATGGGCA AGGACATGGC CAAGATGGGG CCGCCCGCCG CCCGCCGTGA GCAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk2 ( NM_001009929.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001009929.1 Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15973
Clone ID Related Accession (Same CDS sequence)	NM_001009929.3
Accession Version	NM_001009929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570896000000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK2 isoform beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772165.15, AK158574.1 and AL603836.13. On Oct 13, 2012 this sequence version replaced NM_001009929.2. Transcript Variant: This variant (2) lacks a 3' coding exon and contains an additional 3' UTR exon and uses an alternate splice junction at the 5' end of the last exon compared to isoform 3. The resulting isoform (beta) has a shorter and distinct C-terminus compared to isoform gamma. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY533673.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 
CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 
GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 
CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 
AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 
GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 
AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 
AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 
TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 
CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 
TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 
AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 
CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 
ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 
GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 
CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 
CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 
CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 
CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 
CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 
GCCATTGAGA TGGCCCAGCA TGGCCAGAGA TCTCGATCCA TCAGTGGTGC GTCCTCAGGC 
CTTTCTACAA GTCCACTCAG CAGTCCTCGG GTGACCCCTC ACCCCTCACC AAGGGGTAGT 
CCCCTTCCTA CCCCCAAAGG GACGCCTGTC CACACGCCAA AGGAGAGCCC AGCTGGCACA 
CCCAACCCCA CACCACCATC CAGCCCTAGT GTTGGAGGAG TGCCCTGGCG GACACGACTG 
AACTCCATCA AGAACAGCTT CCTGGGCTCA CCTCGATTCC ACCGCCGGAA ACTCCAAGTT 
CCCACGCCAG AGGAGATGTC CAACCTGACC CCAGAATCCT CTCCAGAGCT GGCCAAGAAA 
TCGTGGTTCG GGAACTTCAT CAACCTGGAG AAGGAGGAGC AGATCTTTGT GGTGATCAAG 
GACAAGCCCC TGAGCTCCAT CAAGGCTGAC ATCGTTCATG CCTTCCTGTC GATCCCCAGC 
CTCAGCCACA GCGTTATTTC CCAGACAAGC TTCAGGGCTG AATACAAGGC CACAGGGGGC 
CCAGCAGTGT TCCAGAAGCC GGTCAAGTTC CAGGTGGACA TCACCTACAC TGAGGGCGGA 
GAGGCCCAGA AGGAGAATGG CATCTACTCA GTCACATTCA CTTTACTCTC AGGCCCCAGT 
CGCCGCTTCA AGAGGGTGGT GGAGACCATC CAGGCCCAGC TGTTAAGCAC CCATGACCAG 
CCATCAGCCC AGCACCTGTC AGAACCACCC CCGCCAGCGC CAGGACTAAG CTGGGGTGCT 
GGGCTTAAGG GCCAGAAGGT GGCCACCAGC TACGAGAGTA GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009929.1
CDS	336..2363
Translation	MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSHEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDQPSAQHLSEPPPPAPGLSWGAGLKGQKVATSYESSL

Target ORF information:

RefSeq Version	NM_001009929.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009929.3

ORF Insert Sequence:

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 
CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 
GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 
CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 
AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 
GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 
AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 
AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 
TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 
CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 
TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 
AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 
CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 
ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 
GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 
CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 
CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 
CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 
CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 
CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 
GCCATTGAGA TGGCCCAGCA TGGCCAGAGA TCTCGATCCA TCAGTGGTGC GTCCTCAGGC 
CTTTCTACAA GTCCACTCAG CAGTCCTCGG GTGACCCCTC ACCCCTCACC AAGGGGTAGT 
CCCCTTCCTA CCCCCAAAGG GACGCCTGTC CACACGCCAA AGGAGAGCCC AGCTGGCACA 
CCCAACCCCA CACCACCATC CAGCCCTAGT GTTGGAGGAG TGCCCTGGCG GACACGACTG 
AACTCCATCA AGAACAGCTT CCTGGGCTCA CCTCGATTCC ACCGCCGGAA ACTCCAAGTT 
CCCACGCCAG AGGAGATGTC CAACCTGACC CCAGAATCCT CTCCAGAGCT GGCCAAGAAA 
TCGTGGTTCG GGAACTTCAT CAACCTGGAG AAGGAGGAGC AGATCTTTGT GGTGATCAAG 
GACAAGCCCC TGAGCTCCAT CAAGGCTGAC ATCGTTCATG CCTTCCTGTC GATCCCCAGC 
CTCAGCCACA GCGTTATTTC CCAGACAAGC TTCAGGGCTG AATACAAGGC CACAGGGGGC 
CCAGCAGTGT TCCAGAAGCC GGTCAAGTTC CAGGTGGACA TCACCTACAC TGAGGGCGGA 
GAGGCCCAGA AGGAGAATGG CATCTACTCA GTCACATTCA CTTTACTCTC AGGCCCCAGT 
CGCCGCTTCA AGAGGGTGGT GGAGACCATC CAGGCCCAGC TGTTAAGCAC CCATGACCAG 
CCATCAGCCC AGCACCTGTC AGAACCACCC CCGCCAGCGC CAGGACTAAG CTGGGGTGCT 
GGGCTTAAGG GCCAGAAGGT GGCCACCAGC TACGAGAGTA GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk2 ( NM_001276763.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263692.1 Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu16581
Clone ID Related Accession (Same CDS sequence)	NM_001276763.1
Accession Version	NM_001276763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570896000000
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase BRSK2 isoform delta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772165.15 and AL603836.13. Transcript Variant: This variant (4) has multiple differences in the coding region and 3' UTR, compared to variant 3. The resulting protein (isoform delta) is shorter than isoform gamma. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JF707772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 
CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 
GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 
CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 
AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 
GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 
AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 
AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 
TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 
CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 
TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 
AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 
CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 
ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 
GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 
CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 
CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 
CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 
CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 
CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 
GCCATTGAGA TGGCCCAGCA TGGCCAGAGT AAAGCAGTGT TCAGTAAAAG CCTGGATATC 
GCTGAAGCCC ATCCCCAATT CAGCAAAGAA GACAGATCTC GATCCATCAG TGGTGCGTCC 
TCAGGCCTTT CTACAAGTCC ACTCAGCAGT CCTCGGGTGA CCCCTCACCC CTCACCAAGG 
GGTAGTCCCC TTCCTACCCC CAAAGGGACG CCTGTCCACA CGCCAAAGGA GAGCCCAGCT 
GGCACACCCA ACCCCACACC ACCATCCAGC CCTAGTGTTG GAGGAGTGCC CTGGCGGACA 
CGACTGAACT CCATCAAGAA CAGCTTCCTG GGCTCACCTC GATTCCACCG CCGGAAACTC 
CAAGTTCCCA CGCCAGAGGA GATGTCCAAC CTGACCCCAG AATCCTCTCC AGAGCTGGCC 
AAGAAATCGT GGTTCGGGAA CTTCATCAAC CTGGAGAAGG AGGAGCAGAT CTTTGTGGTG 
ATCAAGGACA AGCCCCTGAG CTCCATCAAG GCTGACATCG TTCATGCCTT CCTGTCGATC 
CCCAGCCTCA GCCACAGCGT TATTTCCCAG ACAAGCTTCA GGGCTGAATA CAAGGCCACA 
GGGGGCCCAG CAGTGTTCCA GAAGCCGGTC AAGTTCCAGG TGGACATCAC CTACACTGAG 
GGCGGAGAGG CCCAGAAGGA GAATGGCATC TACTCAGTCA CATTCACTTT ACTCTCAGGC 
CCCAGTCGCC GCTTCAAGAG GGTGGTGGAG ACCATCCAGG CCCAGCTGTT AAGCACCCAT 
GACCAGCCAT CAGCCCAGCA CCTGTCAGGA ATTATCCCGA AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263692.1
CDS	336..2363
Translation	MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSHEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQSKAVFSKSLDIAEAHPQFSKEDRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRTRLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDQPSAQHLSGIIPKS

Target ORF information:

RefSeq Version	NM_001276763.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BR serine/threonine kinase 2 (Brsk2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276763.1

ORF Insert Sequence:

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 
CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 
GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 
CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 
AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 
GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 
AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 
AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 
TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 
CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 
TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 
AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 
CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 
ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 
GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 
CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 
CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 
CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 
CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 
CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 
GCCATTGAGA TGGCCCAGCA TGGCCAGAGT AAAGCAGTGT TCAGTAAAAG CCTGGATATC 
GCTGAAGCCC ATCCCCAATT CAGCAAAGAA GACAGATCTC GATCCATCAG TGGTGCGTCC 
TCAGGCCTTT CTACAAGTCC ACTCAGCAGT CCTCGGGTGA CCCCTCACCC CTCACCAAGG 
GGTAGTCCCC TTCCTACCCC CAAAGGGACG CCTGTCCACA CGCCAAAGGA GAGCCCAGCT 
GGCACACCCA ACCCCACACC ACCATCCAGC CCTAGTGTTG GAGGAGTGCC CTGGCGGACA 
CGACTGAACT CCATCAAGAA CAGCTTCCTG GGCTCACCTC GATTCCACCG CCGGAAACTC 
CAAGTTCCCA CGCCAGAGGA GATGTCCAAC CTGACCCCAG AATCCTCTCC AGAGCTGGCC 
AAGAAATCGT GGTTCGGGAA CTTCATCAAC CTGGAGAAGG AGGAGCAGAT CTTTGTGGTG 
ATCAAGGACA AGCCCCTGAG CTCCATCAAG GCTGACATCG TTCATGCCTT CCTGTCGATC 
CCCAGCCTCA GCCACAGCGT TATTTCCCAG ACAAGCTTCA GGGCTGAATA CAAGGCCACA 
GGGGGCCCAG CAGTGTTCCA GAAGCCGGTC AAGTTCCAGG TGGACATCAC CTACACTGAG 
GGCGGAGAGG CCCAGAAGGA GAATGGCATC TACTCAGTCA CATTCACTTT ACTCTCAGGC 
CCCAGTCGCC GCTTCAAGAG GGTGGTGGAG ACCATCCAGG CCCAGCTGTT AAGCACCCAT 
GACCAGCCAT CAGCCCAGCA CCTGTCAGGA ATTATCCCGA AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SAD-A, a downstream mediator of GLP-1 signaling, promotes the phosphorylation of Bad S155 to regulate in vitro β-cell functions.
Biochemical and biophysical research communications509(1)76-81(2019 01)
Wang K,Liu D,Zhang Y,Chang X,Xu R,Pang J,Li K,Sun P,Zhu Y,Han X

SAD-A Promotes Glucose-Stimulated Insulin Secretion Through Phosphorylation and Inhibition of GDIα in Male Islet β Cells.
Endocrinology159(8)3036-3047(2018 08)
Nie J,Sun C,Chang Z,Musi N,Shi Y

The loss of the kinases SadA and SadB results in early neuronal apoptosis and a reduced number of progenitors.
PloS one13(4)e0196698(2018)
Dhumale P,Menon S,Chiang J,Püschel AW

Structural insight into the mechanism of synergistic autoinhibition of SAD kinases.
Nature communications68953(2015 Dec)
Wu JX,Cheng YS,Wang J,Chen L,Ding M,Wu JW

LKB1 and AMPK regulate synaptic remodeling in old age.
Nature neuroscience17(9)1190-7(2014 Sep)
Samuel MA,Voinescu PE,Lilley BN,de Cabo R,Foretz M,Viollet B,Pawlyk B,Sandberg MA,Vavvas DG,Sanes JR

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Brsk2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM